Abstract Malaysia, a culturally diverse nation, encompasses various ethnicities, including the Orang Asli, the region’s aboriginal people. Among the Orang Asli tribes in Peninsular Malaysia, the Senoi constitute the largest demographic, followed by the Proto-Malay and Negrito groups, each comprising six distinct subtribes. Traditional methods, such as surveys and interviews, have been instrumental in understanding wildlife consumption practices among indigenous communities like the Senoi. However, they may not always provide accurate and comprehensive data. The adoption of molecular techniques, such as DNA barcoding, presents a promising avenue for enhancing the accuracy and reliability of wildlife consumption studies. This study investigates wildlife consumption practices among the Senoi tribe, particularly the Semoq Beri subgroup in Maran, Pahang, using DNA barcoding that focuses on the Cytochrome C Oxidase Subunit I (COI) gene. Following the collection of biological samples with consent from Semoq Beri villagers, DNA extraction and PCR amplification were performed using established protocols. Subsequently, phylogenetic analysis was conducted to identify wildlife species consumed by the tribe. From the DNA barcoding analysis, two wildlife species were successfully detected: including the Malayan Tapir (Tapirus indicus) and the dusky leaf monkey (Trachypithecus obscurus), commonly consumed by the Semoq Beri tribe. Despite their status conservation, the findings suggest that the Semoq Beri tribe’s hunting practices are relatively sustainable, as they hunt only for subsistence purposes and in small quantities. The study emphasizes the significance of incorporating molecular techniques alongside conventional methods to accurately assess wildlife consumption patterns among indigenous communities. The findings highlight the necessity of collaborative approaches involving indigenous peoples, government agencies, and conservation organizations to preserve traditional knowledge, safeguard wildlife resources, and promote the sustainable coexistence of indigenous communities and biodiversity.
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