Abstract The construction of the new metro line D in Praha-Pankrác provides a unique opportunity to study different aspects such as lithology, stratigraphy and fossil assemblages from the Upper Ordovician and Silurian of the Prague Basin. Results from the sections in tunnels mined so far allowed detailed information about the succession of fossil assemblages, facies variability and actual thicknesses of the upper part of the Bohdalec Formation, the Králův Dvůr and Kosov formations in this part of Prague Basin. The stratigraphic position of Michle Facies in the Bohdalec Formation was also indicated.