Environmental Constraints Research Articles

Continuous evolution of user-defined genes at 1 million times the genomic mutation rate

When nature evolves a gene over eons at scale, it produces a diversity of homologous sequences with patterns of conservation and change that contain rich structural, functional, and historical information about the gene. However, natural gene diversity accumulates slowly and likely excludes large regions of functional sequence space, limiting the information that is encoded and extractable. We introduce upgraded orthogonal DNA replication (OrthoRep) systems that radically accelerate the evolution of chosen genes under selection in yeast. When applied to a maladapted biosynthetic enzyme, we obtained collections of extensively diverged sequences with patterns that revealed structural and environmental constraints shaping the enzyme’s activity. Our upgraded OrthoRep systems should support the discovery of factors influencing gene evolution, uncover previously unknown regions of fitness landscapes, and find broad applications in biomolecular engineering.

A limiting integrative effort plastic waste in the sea of Indonesia and China: The Penta Helix model perspective

Indonesia ranks as the second-largest source of plastic garbage in marine areas, behind China. This is a critical problem that emphasises the need for synergistic endeavors to safeguard the long-term viability of marine ecosystems. The objective of this work is to examine the implementation of the Penta Helix model in the management of marine plastic trash. For this purpose, a Systematic Literature Review (SLR) was carried out, utilizing scholarly papers sourced from the Science Direct, Scopus, and Web of Science databases. The analysis centred on evaluating the Penta Helix model as a cooperative framework for tackling plastic waste management in the marine environments of Indonesia and China. The results suggest that the Penta Helix methodology successfully enables the amalgamation of many interests and resources, making a valuable contribution to the mitigation of plastic pollution in the waters of both nations. In order to advance a more comprehensive and sustainable approach to plastic waste management, this multidisciplinary plan brings together stakeholders from government, academia, business, civil society, and the media. Under this framework, the government is responsible for formulating laws, guidelines, and programs to decrease the use of disposable plastics and improve waste management infrastructure, all while guaranteeing adherence to environmental constraints. Simultaneously, the industrial and academic sectors are responsible for creating sustainable technology and pioneering business strategies, while civil society, in collaboration with the media, has a crucial role in increasing public consciousness regarding the destructive effects of plastic trash. This comprehensive strategy emphasizes the need of synergistic endeavors in tackling the intricate issues of marine plastic contamination.

A Study on Future Population Dynamics of Indigenous Earthworm Species in Golaghat District of Assam, India

This study presents a pioneering exploration into the population dynamics of indigenous earthworm species in the Golaghat district of Assam, India, through the lens of mathematical modeling. Recognizing the integral role of earthworms in enhancing soil productivity and ecosystem health, we embarked on a detailed longitudinal analysis spanning from 2018 to 2023 to assess their population trends across different seasons and subdivisions within the district. Utilizing the principles of Mathematical Biology, we employed the Malthus Growth model and the Logistic Growth model to estimate future population trajectories of earthworm species, integrating biological insights with mathematical rigor. Our methodology, combining extensive field data collection with sophisticated mathematical modeling, provides a replicable framework for similar ecological studies. This study contributes to the growing field of Mathematical Biology, offering a novel approach to understanding the complex interactions within ecosystems and the impact of environmental changes on key species. The outcomes offer valuable insights into sustainable agricultural practices and biodiversity conservation in tropical and subtropical regions, emphasizing the critical role of indigenous earthworm species in maintaining soil health and ecosystem services. Our findings reveal significant seasonal variations and highlight the resilience of these species in the face of ecological changes. The models suggest distinct population growth patterns, with the Logistic model providing a more realistic projection considering environmental constraints and resource availability. The bifurcation diagram is drawn for the Logistic map that presents the phenomena inside the chaotic region.

The Moderating Influence of School Achievement on Intelligence: A Cross-National Comparison.

Education-related variables are positively associated with intelligence in both causal directions, but little is known about the associations' underlying genetically and environmentally intertwined processes and many 'third variables' are probably involved too. In this study, we investigated how school achievement, measured by grade point average (GPA), moderated intelligence test score variation in young adulthood in broadly representative samples from the U.S. state of Minnesota, Denmark, and Germany, attempting to improve both understanding of the importance of environmental contexts and the limitations of currently available modelling techniques to help remedy them. School achievement was positively associated with intelligence test scores in all three contexts, but it moderated variances differently, even within the two cohorts comprising the Minnesota sample. One Minnesota cohort and the German sample suggested that shared environmental variance was larger among individuals with extreme GPAs, while the Danish sample suggested that this was only true among individuals with low GPAs. In contrast to these observations, the other Minnesota cohort suggested that genetic and non-shared environmental variances were greater among individuals with high GPAs. These observations indicated that underlying individual developmental processes and population-level impacts differed. However, our statistical models did not capture these differences clearly. The ways in which they failed all suggested the model limitations involve an inability to address degrees to which environmental constraints restrain social movements that are confounded with individual variations in capacities to move within society.

Regional digital infrastructure and carbon neutrality: A technology–structure–efficiency perspective

The synergistic development of digital transformation and carbon neutrality is a key path to global sustainable development. This study utilized the Broadband China pilot policy and a multi-period Difference-in-Differences (DID) design to comprehensively evaluate the impact of digital infrastructure on carbon neutrality using panel data from 291 prefecture-level cities in China from 2008 to 2021. The results indicate that regional digital infrastructure contributed to carbon neutralization innovation, carbon-intensive industry innovation, and carbon-emission efficiency in the pilot areas. This held true after analyzing the results with propensity score matching, coarsened exact matching, robust estimators, and instrumental variable regression. Specifically, the results of the mediation effect model indicate that digital infrastructure promotes the process of carbon neutrality through several mechanisms, including promoting the rationalization of industrial structure, promoting digital economic development, and inducing innovation in digital technology. In addition, cities with better economic development, lower resource dependence, and weaker environmental constraints have a greater carbon neutrality dividend effect from regional digital infrastructure. These findings indicate a need to optimize policies to unleash the dividends of digital infrastructure in carbon neutrality, which can drive technological, structural, and efficiency changes through digital empowerment. In addition, cities with heterogeneous endowments need to develop targeted measures.

Combining GWAS and RNA-Seq approaches identifies the FtADH1 gene for drought resistance in Tartary buckwheat

Drought is one of the major environmental constraints that significantly affects seedling emergence, yield, and quality of Tartary buckwheat, thereby hindering the development of its industry. However, the molecular mechanisms underlying drought tolerance genes in Tartary buckwheat remain largely unexplored. Alcohol dehydrogenase (ADH), one of the essential plant proteins, plays a crucial role in growth, development, and stress responses, but its specific role in drought resistance is still unclear. In this study, we identified an ADH gene FtADH1, using a membership function value of drought tolerance (MFVD) combined with a genome-wide association study (GWAS) and transcriptomic profiles that confers drought tolerance in Tartary buckwheat. Our findings demonstrated that the overexpression of FtADH1 in Arabidopsis and Tartary buckwheat hairy roots enhances drought tolerance by promoting root elongation and mitigating elevated levels of reactive oxygen species (ROS). Our findings demonstrated that FtADH1 can enhanced tolerance to drought stresses in both Tartary buckwheat and Arabidopsis. This study identifies the FtADH1 as a new player in affecting ROS level and the stress response of Tartary buckwheat by regulating protective enzyme activities at a high level to scavenge ROS and modulating root growth under drought stress. Further, we identified proteins interacting with FtADH1 through a prokaryotic expression pull-down assay combined with mass spectrometry, revealing that FtADH1 specifically interacts with the S-adenosyl-L-methionine (SAM) synthetase protein, FtSAMS1. Overexpression of FtSAMS1 was found to enhance ADH enzymatic activity, leading to increased SAM content in overexpressing Tartary buckwheat hairy roots under water-deficit conditions. Additionally, FtSAMS1 overexpression induced a drought-resistant phenotype in Arabidopsis and Tartary buckwheat hairy roots under drought stress, revealing the biological function of FtADH1. Evolutionary analysis indicates that ADH1 in Fagopyrum species has undergone significant evolutionary events, including duplication and purifying selection, which may contribute to functional diversification and adaptive advantages such as drought resistance in cultivated buckwheat. In summary, this study proposes that FtADH1 is a key contributor to drought tolerance, and its interaction with FtSAMS1 holds potential for the development of drought-resistant varieties in Tartary buckwheat and its relative species.

Plastic waste in dense asphalt mixes for road pavements

Recycling plastic waste in asphalt pavements records increasing statistic figures of application during the last decade worldwide. Due to environmental constraints, but also, to some beneficial properties of plastic waste, recycling in asphalt tends to become current practice, in several countries. In the Aristotle University of Thessaloniki (AUTH), the first attempt to produce asphalt mix using plastic waste consisted of mixing recycled plastic flakes with non-modified binder. Following an asphalt mix design in the laboratory, the whole mass of conventional aggregates was replaced by PET flakes. Recycled PET flakes were chosen to be introduced in the asphalt mix since they are hard, resilient and water repellent. Bitumen emulsion was first preferred as a binder to produce a cold asphalt mix. At a second stage of experimental research, plastic flakes replaced a part of the limestone sand while conventional asphalt 50/70 was used as a binder. At this stage, the experimental research provided more encouraging outcomes. Stability of asphalt mixes decreases as the plastics content in the mix increases, but all recorded values were still within limits of acceptance. At a third stage of research, LDPE recycled material of fine gradation was used to replace a part of the asphalt binder in dense mixes. The objective was to produce a modified binder and a more durable asphalt structure. Test results showed a significant improvement of the performance of asphalt mixes, in terms of strength and deformability of the asphalt mix structure. The research is completed by comparing the conventional techniques with the outcomes of the recycling process and by delineating potential fields of application of the recycled plastics in asphalt pavements.

Green Power Generation from the Tigris River Using Pressure Retarded Osmosis Process

Sustainability including renewable energy and green power, is one of the important feature in recent years due to environmental constraints and the emission of CO2 from fossil fuel. Pressure retarded osmosis (PRO) process is considered one of the effective technology for power generation. This study assessed the application of pressure retarded osmosis to produce power from Tigris River water in Baghdad City, Iraq. Spiral wound TFC membrane was tested in the PRO process with different variables. The effect of different types of draw solutions (MgCl2, NaCl, Sodium Formate, KCl, Sodium Acetate), applied pressure (0 – 7bar), and draw solution concentration (0.08 and 0.4M) were tested in this work. The flux, recovery, and power density for the five draw solution in the order MgCl2>NaCl>sodium formate>KCl>sodium acetate, while the reverse solute flux in the order MgCl2<sodium acetate<KCl<sodium formate<NaCl. Experimentally the membrane pure water permeability (A) was found to be 4.87L/m2.h.bar. The result revealed that the reverse solute flux and the solute permeability coefficient for MgCl2 was 0.55g/m2.h and 8.78×10-8m/s. The flux, recovery, and power density for MgCl2 with a concentration of 0.4M and applied pressure of 7bar was 1.69 LMH, 19%, and 0.33W/m2, respectively.

Network Bottlenecks and Task Structure Control the Evolution of Interpretable Learning Rules in a Foraging Agent.

Developing reliable mechanisms for continuous local learning is a central challenge faced by biological and artificial systems. Yet, how the environmental factors and structural constraints on the learning network influence the optimal plasticity mechanisms remains obscure even for simple settings. To elucidate these dependencies, we study meta-learning via evolutionary optimization of simple reward-modulated plasticity rules in embodied agents solving a foraging task. We show that unconstrained meta-learning leads to the emergence of diverse plasticity rules. However, regularization and bottlenecks in the model help reduce this variability, resulting in interpretable rules. Our findings indicate that the meta-learning of plasticity rules is very sensitive to various parameters, with this sensitivity possibly reflected in the learning rules found in biological networks. When included in models, these dependencies can be used to discover potential objective functions and details of biological learning via comparisons with experimental observations.

Evaluating a Polyhouse Pond-based Aquaculture Production System for Cultivating Neolissochilus hexagonolepis (Chocolate Mahseer) in the Agro-climatic Conditions of Meghalaya, Northeast India

This study evaluated a polyhouse pond aquaculture system (PPAS, 200m2) for cultivating chocolate mahseer (Neolissochilus hexagonolepis) under Meghalaya’s agro-climatic conditions. Over two years, chocolate mahseer growth, survival, and biomass production in PPAS were compared against a conventional open pond aquaculture system (OPAS, 200m2). PPAS demonstrated substantial advantages, with fish exhibiting 34.6% higher final weight (112.24 ± 7.10g vs 83.38 ± 9.91g), 34.8% greater daily weight gain (0.31 ± 0.02 g/day vs 0.23 ± 0.03 g/day), 7.8% higher specific growth rate (1.10 ± 0.04 %/day vs 1.02 ± 0.08 %/day), and 16.1% better survival rate (83.85 ± 5.02% vs 72.20 ± 6.85%) compared to OPAS. Consequently, PPAS total fish biomass (941.97 ± 91.20 kg/ha) was 56.5% greater than OPAS (601.84 ± 90.60 kg/ha). PPAS maintained an average 2.4°C higher water temperature, with a 3.2°C difference during winter. Despite higher initial investment (₹ 85,000 vs ₹ 50,000) PPAS net profit (₹ 6,872) was substantially higher than OPAS (₹ 2,432) due to increased biomass. This innovative system mitigates environmental constraints, meriting further research for enhancing economic viability and long-term sustainability in adverse climatic regions.

From Ignorance to Empowerment: Education’s Role in Sujatha Gidla’s Ants Among Elephants

This research paper mainly focuses on Sujatha Gidla’s Ants Among Elephants, which thoroughly explores the systematic oppression and tenacity prevalent in the Indian social structure, specifically focusing on the caste system. The comprehensive study analyses how personal decisions and environmental constraints determine identity in the context of extensive societal hierarchy. Gidla, from an academic perspective, explores the significant impact of education in promoting empowerment and upward social mobility among marginalised communities. Inspired by Indian scholars who take an interdisciplinary approach to Dalit feminism and by the lens through which Hooks interprets Black feminism. Using intersectionality, this study investigates how caste, gender, and socioeconomic issues affect Dalits in the caste system scenario to highlight their marginalization.

Enterprise green supply chain management under the dual-carbon background: A case study of Geely Automobile

With the severe challenge of global climate change and the increasing constraints of resources and environment, China has put forward the goals of “carbon peaking” and “carbon neutrality” (referred to as “dual carbon”), aiming to promote green economic transformation and high-quality development. Green supply chain management, as an important means to achieve the goal of “double carbon”, is gradually becoming a key path for the sustainable development of enterprises. this study takes Geely Automobile as an example, an in-depth analysis of its green supply chain management practice under the background of “dual carbon”, discusses its successful experience and existing problems, and puts forward suggestions for further optimization, to provide reference and reference for other Chinese enterprises, and promote the green transformation and sustainable development of the entire industry.

The constraints of sedimentary environment on the evolution of bauxite reservoir characteristics within the benxi formation in the Linxing area, Ordos Basin, China

AbstractThe breakthrough of bauxite natural gas in the Taiyuan Formation in the Longdong area of the Ordos Basin makes bauxite natural gas gradually become a new exploration direction. However, the complex sedimentary conditions and diverse developmental strata of the Linxing region present difficulties for the exploration of bauxite gas. In this research, samples of bauxite cores from significant drilling sections in the Linxing area were obtained for comprehensive geochemical, mineralogical, and pore structure analysis. This study investigates the impact of sedimentary environments on the physical properties of bauxite reservoirs within the Benxi Formation, focusing on lithofacies categorization, mineral composition, and pore structure. This study established a lithofacies categorization system that iron minerals, aluminum minerals + titanium minerals, clay minerals as three end‐member components. The study identified four lithofacies types: pure bauxite, clayey bauxite, mixed bauxite, and bauxite mudstone. According to geochemical analysis, the bauxite rock in the study area is deposited in a semi‐enclosed bay with terrestrial freshwater input. The physical properties of bauxite reservoir in the study area are relatively poor, among which the physical properties of bauxite reservoir are the best. Authigenic minerals like pyrite are often filled in dissolution pores. The constraint of sedimentary environment on bauxite reservoir is mainly manifested as the influence on the formation of diaspore mineral framework, and its acidity and alkalinity are very important for the formation of diaspore. Hence, diaspore commonly forms in the shallow lake environments characterized by pure bauxite rock, exhibiting favorable reservoir conditions. This research examines the impact of mineral composition on bauxite reservoirs through the lens of lithofacies and sedimentary environments. The variations in bauxite rock reservoirs across distinct sedimentary environments have been elucidated. It provides a new guidance for the exploration of natural gas in Benxi Formation bauxite in Ordos Basin.

Exploring the effects of waste plastic aggregate on styrene-butadiene-styrene-modified asphalt binders for sustainable rural pavements

In addressing the imperative need for sustainable and cost-effective solutions in rural pavement development, this study navigates the intricate balance of environmental and financial constraints to ensure the resilience of infrastructure in communities with limited resources. The focal point is the integration of waste plastic aggregate (WPA) into hot mix asphalt, augmented by the inclusion of styrene-butadiene-styrene (SBS) for an elevated level of performance. The findings underscore a gradual decrease in the tensile strength ratio, emphasizing a manageable impact, transitioning from 82.4% in the control to 73.7% at 6% WPA. Noteworthy is the observation of marginal reductions in indirect tensile strength and stiffness, particularly notable at higher WPA levels. Dynamic modulus testing highlights susceptibility to rutting at lower frequencies, while high-frequency results demonstrate stability up to 6% WPA. The Hamburg wheel tracking test signals heightened rutting at 3% and 6% WPA, indicating potential challenges in deformation resistance. Despite a slight dip in strength, the discernible magnitude of this reduction is not substantial. This affirms that the incorporation of WPA achieves a harmonious enhancement of sustainability without compromising critical mechanical properties.

Exploration of the Path of Industrial Structure Adjustment Forced by the Rigid Constraint of Water Ecology in the Yangtze River Economic Belt

Considering the two-way influence of water ecology and industrial structure, decompose and quantify the water ecological rigidity constraints into water environment constraints and water resources constraints, construct the evaluation system based on the theoretical framework of PSR, and analyze the spatio-temporal evolution characteristics of water ecological rigidity constraints of the Yangtze River Economic Belt from 2010 to 2021 by using the entropy weighting method, the method of catastrophe progression, and the kernel density estimation. Then, putting forward the types of zoning in the Yangtze River Economic Zone through K-means algorithm analysis to explore the rigid constraints of water ecology to force the path of industrial structure adjustment. The results indicate that: (1) the water ecological rigidity constraints show a decreasing trend, and the decreasing amplitude is upstream &gt; downstream &gt; midstream; (2) the water ecological rigidity constraints generally show the characteristic of “gradually decreasing from east to west”, and the degree of regional differences first decreases, then increases, and then decreases again; (3) it can be divided into six types, and the zoning results gradually change from the concentration in the medium-high type and high-high type to the concentration in the low-low type and medium-low type, and the rigidity constraint of water ecology is obviously improved. In light of the findings, the Yangtze River Economic Belt is divided into five types of development regions and further discusses that the industrial restructuring in different zones has different influencing effects on the regional socio-economic development, which provides suggestions for differentiated paths of water ecological protection and industrial restructuring.

Sustainability Assessment of Agricultural Waste Biogas Production System in China Based on Emergy and Carbon Evaluation Methods

Biogas production is widely recognized as an effective solution for addressing agricultural waste treatment in rural areas. However, its development is often hindered by economic and environmental constraints. This study combined emergy evaluation and carbon footprint analysis methods to establish a new environmental radius assessment model for evaluating the ecological performance and optimization direction of an agricultural waste biogas production system, using a biogas production company in China as a case study. Compared with the straw return model and straw power generation model, the results of emergy indicators and carbon accounting showed that the biogas production model had a lower environmental load and higher economic output and level of emergy sustainability. Additionally, the biogas production system was found to reduce 0.47 kg of carbon emissions per 1 kg of agricultural waste utilized. The application of the biogas production model in rural areas had high ecological sustainability and carbon emission reduction benefits. Environmental radius assessment results confirmed that the reasonable changes in resource collection distance could further enhance the ecological sustainability, carbon mitigation ability, and economic benefits of the biogas production system. The environmental radius assessment method offers a new approach to the location planning of agricultural waste biogas utilization companies in rural areas.

An Exploratory Study of Contextual Control Modes in Teamwork.

This study investigated the relationship between human team member contextual control and team performance under time constraints. Contextual control modes, which describe different strategies for action selection in dynamic environments, characterize how humans maintain performance under variable demands. Control modes have not yet been studied in teamwork settings. Modeling of the cross-level interaction between team members' control modes and emerging team behaviors can improve understanding of effective teamwork in dynamic environments. A human-subjects study explored the relationship between individual contextual control and team performance. Questionnaires about contextual control were used to elicit individual control modes. Analysis compared team members' control modes and investigated how control modes changed under varying time pressures. Participant's control modes differed in their look ahead horizon, the extensiveness of prior action evaluation, and their prior experience. Many team members shifted control modes during trials, resulting in both convergence and divergence of paired control modes. No effects on communication rate were found due to changes in team members' control modes, but partially significant findings may suggest that the control mode divergence affects performance. Teams can operate in multiple control mode configurations that change dynamically according to context. Further research with an increased sample size is warranted to analyze how time constraints influence team members' control modes and overall teaming processes and whether divergence of team control mode is favorable under time pressures. Further study of contextual control in teams may help improve team design to better support teams in coping with time constraints in dynamic environments.

Diversity, environments, and biogeography of Homoloida (Decapoda: Brachyura) from the Late Jurassic to the present

ABSTRACT The section Homoloida with the sole superfamily Homoloidea comprises both extinct and extant brachyuran crab taxa, with records ranging from the Tithonian (Jurassic) to the present. Examination of fossil and modern occurrence records for this clade provides insight into the biogeography, biodiversity, and environmental preference of the homoloid crabs over time. The fossil record of Homoloidea, comprised of the families Tithonohomolidae, Mithracitidae, Homolidae, Latreilliidae, and Poupiniidae, is global in distribution and peaks in diversity during the uppermost Cretaceous with the most recent fossils known from the Miocene. Modern homoloids are absent from only a handful of regions globally, with particularly high diversity and abundance in the tropical Indo-West Pacific. Comparison of interpreted depositional environments for fossil occurrences and depth records for modern occurrences sheds light on the environmental preferences and potential spatial sampling biases of this clade. By assessing both fossil and modern records of Homoloidea, patterns (e.g., absence from cold-water latitudes, Oligo-Miocene disappearance from the northeastern Pacific) emerge that may have important implications for future study of this clade, particularly in relation to dispersal/turnover events and environmental constraints.

An overview of symbiotic and pathogenic interactions at the fungi-plant interface under environmental constraints

The complex and dynamic interactions between fungi and plants constitute a critical arena in ecological science. In this comprehensive review paper, we explore the multifaceted relationships at the fungi-plant interface, encompassing both mutualistic and antagonistic interactions, and the environmental factors influencing these associations. Mutualistic associations, notably mycorrhizal relationships, play a pivotal role in enhancing plant health and ecological balance. On the contrary, fungal diseases pose a significant threat to plant health, agriculture, and natural ecosystems, such as rusts, smuts, powdery mildews, downy mildews, and wilts, which can cause extensive damage and lead to substantial economic losses. Environmental constraints encompassing abiotic and biotic factors are elucidated to understand their role in shaping the fungi-plant interface. Temperature, moisture, and soil conditions, along with the presence of other microbes, herbivores, and competing plants, significantly influence the outcome of these interactions. The interplay between mutualism and antagonism is emphasised as a key determinant of ecosystem health and stability. The implications of these interactions extend to overall ecosystem productivity, agriculture, and conservation efforts. The potential applications of this knowledge in bioremediation, biotechnology, and biocontrol strategies emphasise the importance of adapting to climate change. However, challenges and future directions in this field include the impacts of climate change, emerging fungal pathogens, genomic insights, and the role of the fungi-plant interface in restoration ecology. Hence, this review paper provides a comprehensive overview of fungi-plant interactions, their environmental influences, and their applications in agriculture, conservation, and ecological restoration.

The Elephant in the Room: Expert Experiences Designing, Developing and Evaluating Data Visualizations on Large Displays

Large displays can provide the necessary space and resolution for comprehensive explorations of data visualizations. However, designing and developing visualizations for such displays pose distinct challenges. Identifying these challenges is essential for data visualization designers and developers creating data visualizations on large displays. In this study, we aim to identify the challenges designers and developers encounter when creating data visualizations for large displays. We conducted semi-structured interviews with 13 experts experienced in creating data visualizations for large displays and, through affinity diagramming, categorized the challenges. We identified several challenges in designing, developing, and evaluating data visualizations on large displays, as well as building infrastructure for large displays. Design challenges included scaling visual encodings, limited design tools, and adopting design guidelines for large displays. In the development phase, developers faced difficulties working away from large displays and dealing with insufficient tools and resources. During the evaluation phase, researchers encountered issues with individuals' unfamiliarity with large display technology, interaction interruptions by technical limitations such as cursor visibility issues, and limitations in feedback gathering. Infrastructure challenges involved environmental constraints, technical issues, and difficulties in relocating large display setups. We share the lessons learned from our study and provide future directions along with research project examples to address these challenges.