Soil reflectance is a cumulative attribute determined by interactions between light (photons) and the physical, chemical, and biological properties of soil. Soil properties such as organic matter, moisture, mineral oxide contents, soil texture, and surface roughness all influence soil reflectance at unique wavelengths. Many standard sample preparation techniques are designed to alter soil properties, so as to homogenize samples to improve the consistency of reflectance data collected. This study aims to quantify the effects of one standard sample preparation activity, drying, and repeated wetting–drying cycles on visible–near-infrared soil reflectance, by collecting reflectance data from nine samples which were dried then wetted for a total of three times. This study demonstrates the major, permanent effects of a drying and wetting cycle on soil reflectance, and then presents a model which can be used to correct for these effects. These results have direct implications for remote sensing activities, soil libraries, and soil spectral libraries. These results show that without correction, data from soil spectral libraries, spectral data collected from stored samples, and spectral data transferred between studies, have limited utility for characterizing soils as they exist in the field. Soil spectral data collected in the lab requires correction before it may be used to predict soil properties in the field, as standard sample handling procedures change intrinsic soil properties and introduce systematic error into these data.
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