New multifunctional materials for aerospace industry with exceptional properties must be tested under various environmental conditions to find out possible scatter factors for evaluated properties. Delamination is a typical damage mode observed for laminated composites. Therefore, reliable information regarding the delamination growth behaviour is needed for all operational environments of an aircraft operated at cryogenic and elevated temperatures. In this paper, delamination crack growth monitoring in a climatic chamber on double-cantilever beam (DCB) specimens using optical devices and acoustic emission (AE) techniques is described. A relationship between cumulative AE energy, events localization, clusters, and crack growth in a plain-weave carbon fibre–reinforced epoxy is investigated under constant displacement rate loading at + 80 °C, and − 55 °C. Test results are evaluated for specimens with multi-walled carbon nanotubes (MWCNT) in the microstructure and for a reference material. The mechanical properties during delamination are represented by fracture toughness GIC, and they are also correlated with the AE data. The elevated test temperature caused a decreased rate of released AE energy. The crack growth in material with more significant fibre breakage caused increase of the AE release rate.