Consistent Differences Research Articles

Associations of breastfeeding duration with serum lipid values from infancy until age 20 years - the STRIP study.

The effect of breastfeeding duration on childhood lipid levels has remained controversial. In this study, we aimed to establish the long-term associations of breastfeeding duration with future levels of total cholesterol, high-density lipoprotein (HDL) cholesterol, non-HDL cholesterol and low-density lipoprotein cholesterol. In addition, we report lipid levels at the age of seven months depending on the child receiving any breastmilk. The sample comprised 999 children participating in the prospective Special Turku Coronary Risk Factor Intervention Project (STRIP). Serum lipid profile was studied at the ages of seven months and 13 months, and annually thereafter until the age of 20 years. Duration of breastfeeding was inquired, and infants were divided into those who received or did not receive any breast milk at the age of seven months (n=533 and n=466, respectively). In addition, breastfeeding duration groups (any breastfeeding for 0-4 months, 4-6 months, 6-9 months, and >9 months) were formed. At the age of seven months infants who at that time received breast milk had higher serum HDL cholesterol (0.95±0.21mmol/l vs. 0.90±0.19 mmol/l; p=0.0018), non-HDL cholesterol (3.38±0.78 mmol/l vs. 3.01±0.67 mmol/l; p<0.001) and total cholesterol levels (4.33±0.80 mmol/l vs. 3.91±0.69 mmol/l; p<0.001) than their peers who did not receive breast milk. From two to 20 years of age serum lipid levels showed no consistent differences between the breastfeeding duration groups. Our long-term data showed that duration of breastfeeding has no consistent associations with serum lipid concentrations in healthy individuals aged two to 20 years. www.clinicaltrials.gov, unique identifier NCT00223600.

Automated analysis of snowmelt from Sentinel-2 imagery to determine variable rate irrigation zones in the American Mountain West

Variable rate irrigation (VRI) is used to save water whilst maintaining crop yields in semiarid regions. A key problem is to be able to inexpensively determine spatial patterns in soil moisture so that VRI zones can be defined. In Southern Idaho, USA, the annual precipitation is low and most fall as winter snow. This research investigates whether snow melt patterns measured using freely available time-series Sentinel 2 imagery from Google Earth Engine can define useful VRI zones for two arable fields (Grace and Rexburg). The normalized difference snow index (NDSI) was computed for each 10 m pixel with snow for all winter images of the fields for 2018–2022. NDSI values were ranked within each image and average ranks were calculated for each month and over several years. The patterns of March NDSI were most similar to patterns in yield and soil moisture observed in previous years. Zones were determined using K-means classification of the mean ranks of March NDSI. Kruskal Wallis H tests showed consistent and significant differences between zones for key soil, plant, and topographic variables. For the Grace site, differences between zones were more consistent in their order of magnitude than VRI zones which were calculated using a labor-intensive method. For the Rexburg site, zones were shown to be better when based on snowmelt data from March 2018 to 2022 rather than just March 2019. It is important to base zones on several years of data because in some years there was no snow observed in the Grace field in March. In locations where the majority of soil moisture comes from snowmelt, basing VRI zones on several years of snowmelt patterns in March is a useful and inexpensive tool for deriving meaningful VRI zones. The code used to automatically extract suitable sentinel images and calculate the NDSI is included so that practitioners can use this approach in other locations.

An overheating criterion for bedrooms in temperate climates: Derivation and application

Adequate sleep is crucial to human health and well-being and elevated night-time temperatures can degrade sleep quality. European countries with temperate climates use temperature thresholds between 25°C and 28°C to identify homes that are overheated. The current UK bedroom threshold of 26°C, is based on one small study, which is now over 45 years old. An extensive literature review indicated that with modern summertime bedding and bedwear, which enables body coverage to be easily adjusted, thermal comfort can be achieved for night-time bedroom temperatures up to approximately 29°C. Temperatures measured in 591 bedrooms during England’s hottest ever summer, 2018, are re-analysed. The prevalences of night-time overheating generated by alternative criteria are compared with the prevalences generated by the established adaptive overheating criterion. Comparisons are made for homes with different dwelling and household characteristics. Finally, a new overheating criterion is proposed based on the mean night-time bedroom temperature, with thresholds between 26°C and 29°C depending on the application of the criterion. The allowable exceedance of the chosen threshold is limited to seven nights between May and September. Adopting thresholds of 27°C for vulnerable households and 28°C for others, 23% (5.5 million) of the main bedrooms in English homes were deemed to be overheated in the hot summer of 2018, far fewer than the 69% obtained using the current UK bedroom criterion. Irrespective of the threshold chosen, there were clear, consistent and significant differences in overheating prevalence depending on dwelling and household characteristics. The proposed new overheating criterion is applicable to unconditioned bedrooms in temperate regions. It seeks to strike a balance between the risk that hot bedrooms will be air-conditioned and the risk of temperatures detrimental to a “good nights’ sleep”. Practical Application A new overheating criterion is proposed to identify overheated bedrooms. It adopts the familiar format of a temperature threshold and an allowable exceedance. It is applicable in temperate climates when people are asleep in unconditioned bedrooms. The criterion is intended to aid the interpretation of night-time temperatures predicted by dynamic thermal models and temperature measurements in existing bedrooms. It is applicable to individuals of different heat sensitivity, the design of new homes or the refurbishment of existing homes. With further testing and refinement, it offers a credible replacement to the existing UK bedroom criterion given in CIBSE Guide A, TM59 and in other guides. It can thus underpin the new overheating regulations, Part O, for the design of new dwellings in England.

Inconsistency in perspective-taking during comprehension.

In order to successfully comprehend referring expressions, a listener must often consider how the speaker's perspective differs from their own. Such consideration of others' perspective is effortful and not always employed. Previous studies disagree about whether executive function predicts perspective-taking use in language comprehension. Furthermore, it is unclear whether or not there are consistent individual differences of perspective-taking ability in comprehension. This study tested participants in three perspective-taking in comprehension tasks and two measures of executive function to determine whether participants show consistency in their perspective-taking ability and whether this ability is predicted by measures of executive function. We found that (1) some but not all perspective-taking in comprehension tasks correlate with one another, and (2) inhibition control and working memory are not linked with any of the three perspective-taking measures. Based on these findings, we conclude that perspective-taking in comprehension may not be a unitary ability.

Holocaust survivors: Health and longevity 70 years later.

Holocaust survivors (HS) alive today form a unique and disappearing population, whose exposure to systematic genocide occurred over 70 years ago. Negative health outcomes were widely documented prior to age 70. We examine the hypothesis that the experience of remote trauma continues to negatively affect health, functional status, and survival between the ages of 85-95. The Jerusalem Longitudinal Study (1990-2022) followed a representative sample of Jerusalem residents born 1920-1921, at ages 85, 90 and 95. Home assessment included medical, social, functional, and cognitive status, and mortality data. Subjects were classified: (1) HS-Camp (HS-C): survived slave-labor, concentration, or death camps; (2) HS-Exposed (HS-E): survived Nazi occupation of Europe; (3) Controls: European descent, outside Europe during WWII. We determined Hazards Ratios (HR), adjusting for gender, loneliness, financial difficulty, physical activity, ADL dependence, chronic ischemic heart disease, cancer, cognitive deficits, chronic joint pain, self-rated health. At ages 85 (n = 496), 90 (n = 524), and 95 (n = 383) the frequency of HS-C versus HS-E versus Controls was 28%/22%/50%, 19%/19%/62%, and 20%/22%/58%, respectively. No consistent significant morbidity differences were observed. Mortality between ages 85-90 and 90-95 years was 34.9% versus 38% versus 32.0%, and 43.4% versus 47.3% versus 43.7%, respectively, with no significant differences in survival rates (log rank p = 0.63, p = 0.81). Five-year mortality adjusted HRs were insignificant for HS-C and HS-E between ages 85-90 (HR 0.87, 95% CI 0.54-1.39; HR 1.14, 95% CI 0.73-1.78) and ages 90-95 (HR 0.72, 95% CI 0.39-1.32; HR 1.38, 95% CI 0.85-2.23). Seventy years following their trauma and suffering during the Holocaust, the significant impairments of health, function, morbidity, and mortality which have accompanied survivors throughout their entire adult life, were no longer observed. Indeed, it is likely that survivors living >85 years old represent a uniquely resilient population of people, whose adaptation to adversity has accompanied them throughout their lives.

Extrudability of cookie batters evaluated with three measurement methods.

For cookie batters, there is a strong interaction between ingredient formulation and processing. Changing the amounts and types of sugar and fat in a recipe to change taste or healthiness influences the type of product formation in a production plant. In this study, the applicability of three measurement methods, a rheometer, a forward extrusion cell, and the Foodtexture Puff Device (FPD), was evaluated to measure the extrudability of cookie batters. When looking at the sugar and fat content, both the rheometer and the FPD proved to be consistent and reliable methods to describe the impact of those ingredients on extrudability. A further optimization of the measurement settings of the FPD may improve the performance of this new method. Although the forward extrusion cell performed well for recipes with an increasing sugar content, it did not yield proper results for recipes with a higher fat content due to a separating fat layer. This also limited the applicability of the forward extrusion cell for different fat types, while the other two methods performed well in that case. The small differences in consistency due to different sugar grain sizes were properly described by the forward extrusion cell and the FPD. Overall, it can be concluded that the rheometer performed best to analyze the extrudability of cookie batters, closely followed by the FPD, of which the measurement settings can be optimized. These methods can also be used in the product development phase.

Could Coaching Improve Consumer Credit Use Behavior? Evidence From a State Program

Financial coaching, a hands-on financial wellness approach, has emerged as a go-to strategy to help clients establish and reach their personal financial goals. We analyzed the borrowing and repayment behavior of 1,790 clients who received financial coaching through a program sponsored by the state of Delaware. Relative to a matched comparison group, financial coaching clients cure 0.24 more delinquent accounts, reduce credit card utilization by 5 percentage points, reduce the number of debts in collections by an additional 0.37 accounts, and have $422 less in credit card debt. Findings also show a 7 percentage point increase in the share of clients with a credit card and a 6 percentage point increase in the share of clients with a student loan. We do not see consistent differences in personal installment loans or mortgage holding. These estimates provide evidence that financial coaching can provide benefits for clients while being provided on a state-wide scale, illustrating the potential of public–private programs to provide services.

Atomic Insight on Inhibition of Fibrillization of Dipeptides by Replacement of Phenylalanine with Tryptophan.

Tryptophan (Trp) conjugates destabilize amyloid fibrils responsible for amyloidoses. However, the mechanism of such destabilization is obscure. Herein the self-assembly of four synthesized Trp-containing dipeptides Boc-xxx-Trp-OMe (xxx: Val, Leu, Ile, and Phe) has been investigated and compared with the existing report on their Phe congeners. Two among them are the C-terminal tryptophan analogs of Boc-Val-Phe-OMe (VF, Aβ18-19) and Boc-Phe-Phe-OMe (FF, Aβ19-20), part of the central hydrophobic region of amyloid-β (Aβ1-42). While Boc-Val-Trp-OMe (VW), Boc-Leu-Trp-OMe (LW), Boc-Ile-Trp-OMe (IW), and Boc-Phe-Trp-OMe (FW) displayed a spherical morphology in FESEM and AFM images, the corresponding phenylalanine-containing dipeptides displayed various fibrous structures. Single-crystal X-ray diffraction (SC-XRD) indicated that peptides VW and IW exhibited structures containing parallel β-sheet, cross-β-structure, sheet-like layer structure, and helical arrangement in the solid state. Interestingly, peptide FW displayed inverse γ-turn conformation (similar to open-turn structure), antiparallel β-sheet structure, columnar structure, supramolecular nanozipper structure, sheet-like layer arrangement, and helical architecture in the solid state. The open-turn conformation and nanozipper structure formation by FW may be the first example of a dipeptide that forms such structures. The minute but consistent differences in molecular packing at the atomic level between Trp and Phe congeners may be responsible for their remarkably different supramolecular structure generation. This molecular-level structural analysis may be helpful for the de novo design of peptide nanostructures and therapeutics. Similar studies by the Debasish Haldar group are reported, but they investigated the inhibition of fibrillization of dipeptides by tyrosine and interactions are expectedly different.

Association between statin therapy dose intensity and radiation cardiotoxicity in non-small cell lung cancer: Results from the NI-HEART study

IntroductionRadiation cardiotoxicity is a dose-limiting toxicity and major survivorship issue for patients with non-small cell lung cancer (NSCLC) completing curative-intent radiotherapy, however patients’ cardiovascular baseline is not routinely optimised prior to treatment. In this study we examined the impact of statin therapy on overall survival and post-radiotherapy cardiac events. MethodsPatients treated between 2015–2020 at a regional center were identified. Clinical notes were interrogated for baseline patient, tumor and cardiac details, and both follow-up cancer control and cardiac events. Three cardiologists verified cardiac events. Radiotherapy planning scans were retrieved for application of validated deep learning-based autosegmentation. Pre-specified Cox regression analyses were generated with varying degrees of adjustment for overall survival. Fine and Gray regression for the risk of cardiac events, accounting for the competing risk of death and cardiac covariables was undertaken. ResultsStatin therapy was prescribed to 59% of the 478 included patients. The majority (88%) of patients not prescribed a statin had at least one indication for statin therapy according to cardiovascular guidelines. In total, 340 patients (71%) died and 79 patients (17%) experienced a cardiac event. High-intensity (HR 0.68, 95%CI 0.50–0.91, p = 0.012) and medium-intensity (HR 0.70, 95%CI 0.51–0.97, p = 0.033) statin therapy were associated with improved overall survival after adjustment for patient, cancer, treatment, response and cardiovascular clinical factors. There were no consistent differences in the rate or grade of cardiac events according to statin intensity. ConclusionsStatin therapy is associated with improved overall survival in patients receiving curative-intent radiotherapy for NSCLC, and there is evidence of a dose–response relationship. This study highlights the importance of a pre-treatment cardiovascular risk assessment in this cohort. Further studies are needed to examine if statin therapy is cardioprotective in patients undergoing treatment for NSCLC with considerable incidental cardiac radiation dose and a low baseline cardiac risk.

Sex differences in psychopathic traits during early adolescence: The role of heart rate

PurposePsychopathy – a condition associated with early, pervasive, and severe antisocial behaviors – has been found to be relatively stable over the life-course. While studies have documented a consistent sex difference in psychopathy, the explanatory mechanism is unclear. MethodsUsing a sample of 452 youth (Mage = 11.92 years, 49.6% female), this study empirically tests whether lower heart rate during a social stress task could serve as a causal mechanism to explain the sex difference in psychopathic traits as measured by the Antisocial Process Screening Device. ResultsResults support prior evidence that males exhibit lower heart rates than females and that lower heart rate is associated with higher levels of psychopathy. However, heart rate during a stressor does not sufficiently explain the sex difference in psychopathic traits overall, although it seems to partially explain the sex difference in impulsivity, a key behavioral component of psychopathy. DiscussionThe findings suggest that more research is needed to understand how sex differences in psychopathy emerge in early life and encourages further research on the age(s) or developmental stages at which low heart rate – a well-replicated biological correlate of antisociality – is relevant to understanding the etiology of psychopathy and other antisocial behaviors.

CONNECTING DESIGN ITERATIONS TO PERFORMANCE IN ENGINEERING DESIGN

AbstractNo matter a system's size, complexity, or domain, iterations are fundamental to its design process. However, there is a duality: iterations are both signs of usefully exploring the system's design space and failure to find an appropriate solution. This ambiguity means that we have not been able to connect teams’ iterating behavior to their design's performance, potentially obscuring a way to influence the design process.As such, our exploratory study unpacks the relationship between design iterations and performance. We observed 88 teams in the 2020 Robots to the Rescue Competition in rich detail. Using logs of 7,956 iterations on a Computer-Aided Design platform, we analyzed how high- and low-performing teams revised their submissions, searching for consistent differences in their behavior. We found significant differences in the iterations’ number, scale, and cadence between these groups of teams. These findings emphasized the correlation between certain iteration patterns and the success of a design: the best teams will likely revise differently than the worst ones. It also showed the importance of a fine-grained, time- dependent view of the design process to resolve open questions in the literature.

Preliminary Reporting Patterns of Suicide Ideation and Attempt Among Native American Adolescents in Two Samples

ABSTRACT Objective Native American adolescents are disproportionately burdened by suicidality. Here, we examine patterns of reporting of suicide ideation and suicide attempt among Native American youth compared to those from other ethnoracial backgrounds, as this data is important for grounding commonly subscribed to frameworks of suicide risk (e.g., ideation-to-action). Method Data are from the Youth Risk Behavior Surveillance Survey (N = 54,243; grades 9–12; 51.0% female) and Minnesota Student Survey (N = 335,151; grades 8, 9, 11; 50.7% female). Comparing Native American youth to peers from other ethnoracial backgrounds, we examined two suicide reporting patterns: 1) odds of reporting suicide attempt among those who reported ideation and 2) odds of reporting suicide ideation among those who reported an attempt. Results Across both samples, when reporting suicide ideation, youth from other ethnoracial backgrounds were 20–55% less likely than Native American youth to also report attempt. While few consistent differences were observed between Native American youth and those from other racial minority backgrounds in patterns of co-reporting suicide ideation and attempt across samples, White youth were between 37% and 63% less likely than Native American youth to report a suicide attempt without also reporting ideation. Conclusions The increased odds of engaging in a suicide attempt with or without reporting ideation question the generalizability of widely held frameworks of suicide risk to Native American youth and have important implications for suicide risk monitoring. Future research is needed to illuminate how these behaviors unfold over time and the potential mechanisms of risk for engaging in suicide attempts in this disproportionately burdened group. Abbreviations: YRBSS: Youth Risk Behavior Surveillance Survey; MSS: Minnesota Student Survey

Structure of cellulose in birch phloem fibres in tension wood: an X-ray nanodiffraction study

BackgroundTo gain a better understanding of bark layer structure and function, especially of the phloem fibres and their contribution to the posture control of trees, it is important to map the structural properties of these cells. The role of bark can also be linked to the reaction wood formation and properties which are essential when it comes to studying the questions related to tree growth. To offer new insights into the role of bark in the postural control of trees, we studied the micro- and nanoscale structures of the phloem and its nearest layers. This study is the first time, in which phloem fibres in trees have been extensively examined using X-ray diffraction (XRD).We determined the orientation of cellulose microfibrils in phloem fibres of Silver birch saplings by using scanning synchrotron nanodiffraction. The samples consisted of phloem fibres extracted from tension, opposite and normal wood (TW, OW, NW).ResultsUsing scanning XRD, we were able to obtain new information about the mean microfibril angle (MFA) in cellulose microfibrils in phloem fibres connected to reaction wood. A slight but consistent difference was detected in the average MFA values of phloem fibres between the TW and OW sides of the stem. Using scanning XRD, different contrast agents (intensity of the main cellulose reflection or calcium oxalate reflection, mean MFA value) were used to produce 2D images with 200 nm spatial resolution.ConclusionsBased on our results, the tension wood formation in the stem might be related to the structure and properties of phloem fibres. Thus, our results suggest that the nanostructure of phloem fibres is involved in the postural control of trees containing tension and opposite wood.

"A lot to manage and still have some kind of a life": How multiple myeloma impacts the function and quality-of-life of Black-White patient-caregiver dyads.

Multiple myeloma (MM) is an incurable debilitating blood cancer associated with the lowest health related quality of life (HRQoL) of all cancers. With nearly 88% of adults aged ≥55 years at diagnosis, age-associated physical losses, comorbidities, and social factors contribute to worsening HRQoL. This qualitative study assessed dyadic (patient-informal caregiver) perspectives on the factors contributing to HRQoL in MM survivors. We recruited 21 dyads from the UNC-Chapel Hill Lineberger Comprehensive Cancer between 11/2021 and 04/2022. Participants completed a single dyadic semistructured interview capturing broad perspectives on MM. We used ATLAS. ti v 9 for project management and to facilitate data analysis using the Sort and Sift, Think and Shift approach (ResearchTalk, Inc.). This iterative approach allowed the exploration and identification of themes within and across transcripts. The mean age at enrollment was 71 years (median: 71, range: 57-90) for patients and 68 years (median 67, range: 37-88) for caregivers. All dyads were racially concordant (11 Black/AA and 10 White). However, we aggregated the findings due to no consistent racial differences. Six themes related to (1) physical burden, (2) treatment challenges, (3) losses of independence, (4) caregiver burden, (5) patient and caregiver perseverance, and (6) adjustment to a new normal were identified. Dyads also experienced MM together, resulting in patients and caregivers experiencing changes in their ability to engage in physical and social activities, which further contributed to poor HRQoL. Patients' increased need for social support led to shifts in the caregiver roles, resulting in caregivers feeling burdened by their responsibilities. All dyads acknowledged the need for perseverance and adaptability to a new normal with MM. The functional, psychosocial, and HRQoL of older patients with MM and their caregivers remain impacted ≥6 months after a new diagnosis highlighting clinical and research opportunities to focus on preserving or improving the health of dyads living with MM.

Decline in positive future orientations among adolescents during covid-19: The role of socioeconomic status, parental support, and sense of control.

Before coronavirus disease (covid-19), adolescents from a lower socioeconomic status(SES) background tend to have less positive future orientations, receive less parental support, and have a weaker sense of control than adolescents from a higher SES background. The covid-19 pandemic has potentially increased the socioeconomic gaps in positive future orientations, parental support, and sense of control among adolescents who are currently in vocational education. As societies are aiming to return back to precovid norms, certain groups of adolescents might require more attention for ensuring a stable future than others. Two-wave questionnaire data of 689 Dutch adolescents (Mage = 17.8; 56% female) from the Youth Got Talent project was analyzed. Latent Change Score models are a relatively novel approach that allows two-wave data to estimate associations between precovid predictor variables and changes in outcome variables from before to during covid-19 (e.g., SES, positive future orientations, parental support, and sense of control). Analyses were preregistered. The precovid socioeconomic differences in adolescent's positive future orientations and sense of control remained stable during covid-19, whereas the socioeconomic difference in parental support decreased during covid-19. A decline in parental support, an increase in sense of control, and more covid-19 hardships were associated with an increase in future orientations. The covid-19 situation has not substantially increased socioeconomic differences in positive future orientations and sense of control, but did decrease socioeconomic differences in parental support among adolescents. Short-term policies should aim to facilitate parental support and positive future orientations to all adolescents who experienced a decline, while also long-term focusing on the more consistent socioeconomic difference in sense of control among adolescents.

Is flight initiation distance associated with longer-term survival in yellow-bellied marmots, Marmota flaviventer?

The distance at which animals move away from threats, flight initiation distance (FID), is often used to study antipredator behaviour and risk assessment. Variation in FID is explained by a variety of internal and external biotic and physical factors, including anthropogenic activities. Most prior studies focused on unidentified individuals, so our understanding of the fitness consequences of FID is relatively limited. We asked whether consistent individual differences in variation in flight initiation distance is associated with variation in summer survival and/or winter survival in an individually marked population of yellow-bellied marmots. We found no clear association between flight initiation distance and summer survival or winter survival. This suggests that FID decisions, while demonstrably optimizing current survival, may not have longer-term fitness consequences. Our results may be explained by the relatively modest repeatability of FID or it may have emerged from our attempt to explain longer-term measures of fitness. Future studies of the fitness consequences of personality traits should pay particular attention to the time interval between measuring the individuality of a trait and examining its fitness consequences.

Effect of cultivar on measurements of nitrate concentration in petiole sap and leaf N content in greenhouse soil-grown cucumber, melon, and sweet pepper crops

Excessive N fertilizer applications in intensive vegetable production in soil is commonly associated with appreciable N losses causing negative environmental impact. Measuring petiole sap [NO3−‒N] and leaf N content (%) are simple and practical monitoring methods to assess crop N status for improving N fertilizer management. The effect of cultivar on petiole sap [NO3−‒N] and leaf N content was evaluated. One cucumber, two melon, and two sweet pepper crops were grown in different cropping periods, with three cultivars in each crop. Three N treatments, deficient (N1), sufficient (N2) and excessive (N3) N supply, were applied by combined fertigation with drip irrigation. For a given N supply, there were often significant differences between cultivars in petiole sap [NO3−‒N] and leaf N content in cucumber, the two melon crops and one pepper crop. This was, particularly so with the sufficient (N2) and excessive (N3) N supply. In the cucumber and two melon crops, there were consistent differences in petiole sap [NO3−‒N] between cultivars in two or three of the different N treatments. In some crops, very little petiole sap [NO3−‒N] was measured with deficient (N1) N supply. In the two pepper crops, the differences between cultivars were less clear than with cucumber and melon. In general, for the three species examined, petiole sap [NO3−‒N] was subject to more consistent and larger effects between cultivars, than was leaf N. Average differences between cultivars in petiole sap [NO3−‒N] of 200‒450 mg NO3−‒N L−1 were observed during periods of 4‒6 weeks in cucumber and melon. The differences between different cultivars of the same species in petiole sap [NO3−‒N] and leaf N content, when receiving the same N supply, has implications for the practical applications of these methods for monitoring crop N status.

Development of Anatomic Risk Factors for ACL Injuries: A Comparison Between ACL-Injured Knees and Matched Controls

Background: Several tibiofemoral anatomic features have been repeatedly associated with increased anterior cruciate ligament (ACL) injury risk. Previous studies have highlighted age and sex differences among these anatomic risk factors, but little is known about the normal and pathologic development of these differences during skeletal maturation. Purpose: To investigate differences in anatomic risk factors at various stages of skeletal maturation between ACL-injured knees and matched controls. Study Design: Cross-sectional study; Level of evidence, 3. Methods: After institutional review board approval, magnetic resonance imaging scans from 213 unique ACL-injured knees (age, 7-18 years, 48% female) and 239 unique asymptomatic ACL-intact knees (age, 7-18 years, 50% female) were used to measure femoral notch width, posterior slope of the lateral and medial tibial plateau, medial and lateral tibial spinal height (MTSH, LTSH), medial tibial depth, and posterior lateral meniscus-bone angle. Linear regression was performed to assess change in quantified anatomic indices with age for male and female patients in the ACL-injured cohort. Two-way analysis of variance with Holm-Sidak post hoc testing was performed to compare anatomic indices between ACL-injured knees and ACL-intact controls in each age group. Results: In the ACL-injured cohort, notch width, notch width index and medial tibial depth increased with age (R2 > 0.1; P < .001) in both sexes. MTSH and LTSH increased with age only in boys (R2≥ 0.09; P≤ .001), whereas meniscus-bone angle decreased with age only in girls (R2 = 0.13; P < .001). There were no other age differences in quantified anatomic indices. Patients with ACL injury consistently had a significantly higher lateral tibial slope (P < .01) and smaller LTSH (P < .001) as compared with ACL-intact controls across all age groups and sexes. When compared with age- and sex-matched ACL-intact controls, ACL-injured knees had a smaller notch width (boys, 7-18 years; girls, 7-14 years; P < .05), larger medial tibial slope (boys and girls, 15-18 years; P < .01), smaller MTSH (boys, 7-14 years; girls, 11-14 years; P < .05), and larger meniscus-bone angle (girls, 7-10 years; P = .050). Conclusion: The consistent morphologic differences throughout skeletal growth and maturation suggest a developmental role in high-risk knee morphology. The observed high-risk knee morphology at an earlier age preliminarily suggests the potential of knee anatomy measurements in identifying those with a predisposition toward ACL injury.

Morphological variation of the Pan talus relative to that of Gorilla.

Differences in talar articular morphology relative to locomotion have recently been found within Pan and Gorilla. Whole-bone talar morphology within, and shared variation among, Pan and Gorilla (sub)species, however, has yet to be investigated. Here we separately analyze talar external shape within Pan (P. t. troglodytes, P. t. schweinfurthii, P. t. verus, P. paniscus) and Gorilla (G. g. gorilla, G. b. beringei, G. b. graueri) relative to degree of arboreality and body size. Pan and Gorilla are additionally analyzed together to determine if consistent shape differences exist within the genera. Talar external shape was quantified using a weighted spherical harmonic analysis. Shape variation both within and among Pan and Gorilla was described using principal component analyses. Root mean square distances were calculated between taxon averages, and resampling statistics conducted to test for pairwise differences. P. t. verus (most arboreal Pan) talar shape significantly differs from other Pan taxa (p < 0.05 for pairwise comparisons) driven by more asymmetrical trochlear rims and a medially-set talar head. P. t. troglodytes, P. t. schweinfurthii, and P. paniscus do not significantly differ (p > 0.05 for pairwise comparisons). All gorilla taxa exhibit significantly different talar morphologies (p < 0.007 for pairwise comparisons). The more terrestrial subspecies of G. beringei and P. troglodytes exhibit a superoinferiorly taller talar head/neck complex. P. t. verus exhibits talar morphologies that have been previously related to more frequent arboreality. The adaptations in the more terrestrial G. beringei and P. troglodytes subspecies may serve to facilitate load transmission.

Creation and characteristics of tetraploid and mixoploid centipedegrass

AbstractPloidy manipulation has been used in many crop improvement programs to develop plant species with wider adaptability and desirable traits. The objectives of this study were to create stable tetraploid centipedegrass (Eremochloa ophiuroides [Munro] Hack.) lines and evaluate them for beneficial traits. To generate polyploid lines, “TifBlair” (2n = 2x = 18) seeds were exposed to gamma radiation and callus was generated using tissue culture, exposed to glyphosate and regenerated into plantlets. After 5 years of continuous propagation, two lines, Hongliang Wang (HW)16 and HW123, were found to be tetraploid, and one line, HW61, was found to be a mixoploid using flow cytometry and chromosome counts. Nuclear DNA contents for diploid and tetraploid lines ranged from 1.97 to 2.10 and 4.14 to 4.30 pg 2C−1, respectively. Ten centipedegrass lines were genotyped using seven simple sequence repeat markers and clustered by origin (derived from TifBlair or the University of Georgia breeding program). Six lines were evaluated for morphological and physiological traits under greenhouse conditions. Trait evaluation showed that tetraploid lines had larger stomata and leaf width and reduced stomatal density. For all other traits evaluated under greenhouse conditions, although significant differences were observed between lines, no consistent differences separated diploid and tetraploid lines. Between the tetraploid lines, HW16 had higher evapotranspiration, percentage green cover, and relative water content than HW123, whereas HW123 had a higher average clipping yield and root width than HW16. In conclusion, irradiation and tissue culture are valuable techniques for the generation of stable polyploid lines, and an increased ploidy level led to larger vegetative structures in centipedegrass.