Consecutive Yr Research Articles

Vegetation and Animal Performance Responses to Stocking Density Grazing Systems in Nebraska Sandhills Meadows

Management-intensive grazing, which is proposed to increase forage and animal productivity and maintain soil integrity and biodiversity, is seen as an alternative to meet 21st century agricultural and environmental challenges. The purpose of this study was to test the hypothesis that high levels of trampling of standing vegetation associated with mob grazing (a.k.a., ultrahigh stocking density) leads to increased plant diversity and productivity. A long-term experiment was established on a subirrigated meadow in the Nebraska Sandhills as a complete block design comparing three grazing treatments applied annually during the growing season for 8 consecutive yr (2010−2017): 120-pasture rotation with one grazing cycle (mob; 225 000 kg live weight ha−1), four-pasture rotation with one grazing cycle (4PR1; 7 000 kg live weight ha−1), and four-pasture rotation with two grazing cycles (4PR2; 5 000 kg live weight ha−1). All treatments were set at a moderate stocking rate (7.4 animal unit months ha−1) using yearling steers. Percentage trampling, plant production, species composition, and steer weight gain were estimated annually. We applied linear mixed-effect models to account for year and treatment effect on the response variables. Percentage trampling on mob pastures ranged from 40% to 55% over the 8 yr of the study, nearly double that of the 4PR1 and 4PR2 pastures. We observed that mob grazing had no overall effect on plant species composition, aboveground production, and root growth relative to low stocking densities. Average daily gain of steers in the mob pastures was less than gain of steers in 4PR2 pastures in all years, with intermediate weight gains for the 4PR1 steers. Overall, stocking density did not appear to be a driver of plant composition and productivity in rotationally grazed pastures on subirrigated meadows in the Nebraska Sandhills.

Kepler Bonus: Aperture Photometry Light Curves of EXBA Sources

Abstract NASA’s Kepler mission observed background regions across its field of view for more than 3 consecutive yr using custom designed superapertures (EXBA masks). Since these apertures were designed to capture a region of the sky rather than single targets, the Kepler Science Data Processing Pipeline produced target pixel files but did not produce light curves for the sources within these background regions. In this work, we produce light curves for 9327 sources observed in the EXBA masks. These light curves are generated using aperture photometry estimated from the instrument’s pixel response function (PRF) profile computed from Kepler's full-frame images. The PRF models enable the creation of apertures that follow the characteristic shapes of the point-spread function in the image and the computation of flux completeness and contamination metrics. The light curves are available at MAST as a high-level science product (kbonus-apexba). Alongside this data set, we present kepler-apertures, a Python library to compute PRF models and use them to perform aperture photometry on Kepler-like data. Using light curves from the EXBA masks, we found an exoplanet candidate around Gaia EDR3 2077240046296834304 consistent with a large planet companion with a 0.81 R J radius. Additionally, we report a catalog of 69 eclipsing binaries. We encourage the community to exploit this new data set to perform in-depth time domain analysis, such as eclipsing binaries demographics and other types of studies.

Effective Management Practices for Increasing Native Plant Diversity on Mesquite Savanna-Texas Wintergrass-Dominated Rangelands

Throughout the Rolling Plains and Cross Timbers ecoregions of Texas, native grassland plant communities have been converted into low-diversity plant communities by long-term, intensive overgrazing by cattle and fire suppression. Much of the historical plant community has become dominated by annuals, Texas wintergrass (Nassella leucotricha), and honey mesquite (Prosopis glandulosa). This degradation has been so severe that many native bird species, including Northern bobwhite (Colinus virginianus), have experienced drastic population declines. We conducted a small-plot study to determine effective management actions for transforming mesquite savanna−Texas wintergrass communities to diverse native plant communities supportive of native wildlife species. We tested multiple management practices following mechanical mesquite brush removal, including seeding, timed treatments of herbicide, prescribed burns, and high-intensity, short-duration cattle grazing. Results indicated that plots receiving early spring treatments of herbicide followed by burning and grazing over 2 consecutive yr best reduced cool-season grasses and promoted overall restoration goals by increasing native warm-season grass and forb establishment.

Long-term winter-site fidelity in Song Sparrows (Melospiza melodia)

AbstractIn migratory animals, the degree to which individuals return to the same wintering sites across multiple years can affect fitness and population dynamics, and thus has important implications for conservation. Despite this, long-term evaluations of wintering-site fidelity are rare for migratory birds: many populations are intensively studied on their breeding grounds but tracking the migratory movements of small birds once they leave the breeding grounds is challenging. To evaluate patterns of overwintering location and fidelity, we collected winter-grown claw tissue from 301 Song Sparrows (Melospiza melodia; 449 samples) captured in spring at their breeding grounds over 6 consecutive yr and assessed stable hydrogen isotope (δ2Hc) values to determine within-individual repeatability and between-year variation in wintering latitudes. We also retrieved useable data from 8 geolocators over 2 consecutive winters. Geolocator-derived wintering positions correlated with origins based on δ2Hc values. Consistent with previous findings, male δ2Hc values reflected more northerly wintering areas than those of females, indicating shorter latitudinal migration distances for males, but the magnitude of the sex difference varied across years. The distribution of wintering latitudes was generally consistent among years, except for the 2015 舑2016 winter, which had unusually negative δ2Hc values. Values of δ2Hc were repeatable for males but not for females, suggesting that winter-site fidelity could differ between sexes. The data presented here emphasize the importance of tracking migratory populations across multiple years to uncover factors affecting population dynamics.

Beaver Habitat Selection for 24 Yr Since Reintroduction North of Yellowstone National Park

Beavers (Castor canadensis) disappeared from drainages north of Yellowstone National Park in the mid-1900s because of trapping, a potential tularemia outbreak, and willow (Salix spp.) stand degradation by ungulates. Beavers were reintroduced in 1986 after a 40–yr absence with inventories of active-beaver structures completed each fall after reintroduction for 24 consecutive yr. We used this inventory to evaluate the expansion of beaver populations in a riparian environment recovering from past overuse by ungulates. Specifically, we investigated the density of active-beaver colonies and dams, the change in willow cover, and habitats associated with beaver expansion since reintroduction. Successful establishment and expansion of beavers indicate that sufficient resources were available to the population despite the suboptimal condition of riparian vegetation. Carrying capacity on third-order streams was reached approximately 14 yr after reintroduction (2000) with an average annual density of 1.33 (95th percentile = 1.23−1.44 active colonies/stream km) between 2000 and 2010. The average annual density of beaver dams during this time was 2.37 (2.04−2.71 active dams/stream km). Despite the beaver population being at carrying capacity in meadows since 2000, willow cover increased by 16% between 1981 and 2011. We speculate that beaver activities, together with reduced ungulate browsing from predation and habitat loss, combined to increase willow cover. Willow cover and height were positively associated with colony longevity, but numerous other influencing variables included secondary channels, sinuosity, stream depth, and sandbar width. Our results provide evidence that beaver reintroduction can be successful in riparian areas where willow stand condition is less than optimal and that beavers might ultimately improve willow condition. We suggest that reducing ungulate use of overgrazed riparian environments will facilitate the reestablishment of beaver populations. We also provide managers with habitats that should be identified in an environment targeted for reintroduction.

572 Evaluation of Brassica carinata meal as a protein supplement for growing beef heifers

Brassica carinatais a new oilseed crop in Florida with the potential of producing high-quality biodiesel for use as jet biofuel. A high-protein meal (∼40% CP) is obtained as a byproduct of oil extraction, however, this meal has not been tested as a potential supplement for growing beef cattle. The objective of this experiment was to determine the effects of supplementation withBrassica carinatameal (BCM) on animal performance, attainment of puberty, and blood profile in growing beef heifers consuming bermudagrass hay (Cynodon dactylon). Sixty-four Angus crossbred heifers (240 ± 39 kg initial BW) were stratified and blocked (2 blocks: light and heavy) by initial BW and randomly allocated into 18 pens over 2 consecutive yr (10 pens in yr 1 and 8 pens in yr 2). Within block, pens were randomly assigned to one of two treatments: 0 (CTL) or 0.3% of BW/d (as fed) of BCM pellets. All heifers were provided ad libitum access to bermudagrass hay and water, and BCM pellets were supplemented daily in the pen. Body weight and blood samples were collected weekly for 70 d, before the daily supplementation. Plasma was analyzed for concentrations of progesterone, triiodothyronine (T3), and thyroxine (T4). Data were analyzed as a generalized randomized block design including the fixed effects of treatment, wk, treatment × wk, block, and block × treatment, and the random effect of year. Repeated measures, with pen within year as subject, were used to analyze T3 and T4 concentrations over time. A survival analysis was conducted to determine time to attainment of puberty. There was a difference (P< 0.01) in ADG between CTL (0.14 kg) and BCM (0.42 kg). There was no treatment or block (P>0.05) effect for concentrations of T3 and T4; however, there was a difference in wk (P<0.01). Time to attainment of puberty did not differ between treatments (P= 0.68); however, there was an effect of block (P<0.01) where the light block heifers attained puberty earlier compared to the heavy block heifers. FeedingB. carinatameal as a protein supplement at 0.3% of BW/d is a viable option for increasing ADG of growing beef heifers, without affecting the attainment of puberty.

Seed Bank Depletion: The Key to Long-Term Downy Brome (Bromus tectorum L.) Management

Invasive winter annual grasses such as downy brome (Bromus tectorum L.) are a threat to native ecosystems throughout the United States. Downy brome is able to exploit moisture and nutrients throughout the fall and early spring before native plants break dormancy. This results in decreased native species abundance and development of monotypic downy brome stands. Short-term downy brome management has been shown to be effective; however, the soil seed reserve has often been overlooked, although it’s the mechanism responsible for rapid reestablishment. This field study was conducted at two sites in Colorado to evaluate the longevity of the downy brome soil seed reserve and its implications on long-term downy brome control. Glyphosate plus adjuvant applications were made for 0, 1, 2, 3, 4, or 5 consecutive years. Downy brome and perennial grass biomass harvests were conducted yearly to determine changes in species composition. In addition, soil cores were collected to evaluate the yearly variation and depletion of the downy brome soil seed bank in response to consecutive glyphosate applications. We found that 1−3 yr of consecutive glyphosate treatments were insufficient to deplete the downy brome soil seed bank. Downy brome biomass and the soil seed bank recovered within 1−2 yr after glyphosate treatments were terminated; however, 4 and 5 consecutive yr of glyphosate applications were sufficient to control downy brome through depletion of the soil seed bank. Managing downy brome for 4−5 consecutive yr resulted in a 4- to 9-fold increase in perennial grass biomass. These data suggest that long-term management of downy brome is dependent on eliminating the soil seed bank using a multiyear approach.

Native-exotic richness relationships: a biogeographic approach using turnover in island plant populations.

Spatial variation in exotic species richness is often correlated with native species richness, for reasons that are poorly understood. To better understand the mechanisms underpinning native-exotic richness relationships, I quantified the colonization and extinction of 18 exotic and 16 native plant species on 39 small islands located off the coast of New Zealand for 8 consecutive yr. Results revealed a positive native-exotic richness relationship, which could be explained by similar demographic responses of native and exotic species to island area. However, native and exotic species showed subtle differences in their response to other island attributes. Turnover in native species declined with island isolation, whereas turnover in exotic species increased with the exposure of islands to ocean-borne disturbances. Overall results illustrate how long-term observations of species turnover can be used to better understand the mechanisms underpinning native-exotic richness relationships, and demonstrate that large, exposed islands can be especially susceptible to invasions by exotic species.

Burning Controls Barb Goatgrass (Aegilops triuncialis) in California Grasslands for at Least 7 Years

AbstractBarb goatgrass is an invasive annual grass from the Mediterranean region that negatively affects both native plant biodiversity and the forage quality of grasslands. Prescribed burning may be the best landscape-level tool available to manage invasive species like barb goatgrass while also enhancing biodiversity, but few studies have quantified the long-term effects of fire on goatgrass and the rest of the plant community. We assessed the effects of fire on an invading front of barb goatgrass on a private ranch in Sacramento County, CA. We established burned and unburned treatment plots within the goatgrass-infested area and used prescribed fire to burn the treatment plots in June 2005. We monitored plant-community composition before burning and for 7 consecutive yr following the burn. Additionally, we tested the viability of goatgrass seeds in both burned and unburned plots. One year after the burn, goatgrass cover in burned plots was 3% compared with 21% in unburned plots. This reduction in goatgrass cover was still strong 2 yr after the burn (burned, 6%; unburned, 27%) and weaker but still statistically significant for 4 of the next 5 yr. The burn also reduced germination of goatgrass seed by 99% as indicated by seed-viability tests conducted in the laboratory. The native plant community responded positively to the burn treatment in the first year following the burn with an increase in native diversity in burned plots vs. unburned plots, but the effect was not detectable in subsequent years. Nonnative annual forb species cover also increased in the first year following the burn. Our study shows that a single springtime burn can result in a short-term boost in native species diversity, reduced seed germination of barb goatgrass to near zero, and reduced cover of barb goatgrass for at least 7 yr after the burn.

Twelve Years of Stover Removal Increases Soil Erosion Potential without Impacting Yield

Corn (Zea mays L.) stover (non-grain aboveground biomass) in the US Corn Belt is used increasingly for livestock grazing and co-feed and for cellulosic bioenergy production. Continuous stover removal, however, could alter long-term agricultural productivity by affecting soil organic C (SOC) and soil physical properties, indicators of soil fertility and erosion potential. In this study, we showed that 12 consecutive yr of 55% stover removal did not affect mean grain yields at any N fertilizer rate (4.5, 6.3, and 6.0 Mg ha−1 for 60, 120, and 180 kg N ha−1 yr−1, respectively) in a marginally productive, rainfed continuous corn system under no-till (NT). Although SOC increased in the top 30 cm of all soils since 1998 (0.54–0.79 Mg C ha−1 yr−1), stover removal tended to limit SOC gains compared with no removal. Near-surface soils (0–5-cm depth) were more sensitive to stover removal and showed a 41% decrease in particulate organic matter stocks, smaller mean weight diameter of dry soil aggregates, and lower abundance of water-stable soil aggregates compared with soils with no stover removal. Increasing N fertilizer rate mitigated losses in total water-stable aggregates in near-surface soils related to stover removal. Collectively, however, our results indicated soil structure losses in surface soils due to lower C inputs. Despite no effect on crop yields and overall SOC gains with time using NT management, annually removing stover for 12 yr resulted in a higher risk of wind and water erosion at this NT continuous corn site in the western Corn Belt.

Nutritive Value of Field Bindweed (Convolvulus arvensis) Roots as a Potential Livestock Feed and the Effect of Aceria malherbae on Root Components

Crop producers might be able to better manage field bindweed, an aggressive perennial weed, by utilizing tillage to bring roots to the surface where they can be consumed by ruminant livestock. The objectives of this study were to provide first perspectives on forage nutritive value of field bindweed roots and to determine root chemistry responses to Aceria malherbae, an eriophyid mite that has been released for field bindweed biocontrol in the western United States and Canada. To accomplish these objectives, root systems were sampled from A. malherbae-infested and noninfested plants occurring in an agricultural field in eastern New Mexico. Sampling took place during autumn and spring of each year for 3 consecutive yr. Results indicated that A. malherbae reduced taproot diameter and increased root concentrations of Ca, P, and Mg. However, A. malherbae did not affect root concentrations of acid detergent fiber, nonfiber carbohydrates, neutral detergent fiber (NDF), crude protein (CP), and total digestible nutrients (TDN). Overall means for NDF (33.8%), CP (11.6%), and TDN (72.1%) were similar to those reported for forages commonly grown in the region, suggesting that field bindweed roots might positively contribute to nutritional programs of ruminant livestock. These results justify subsequent studies on livestock responses to field bindweed roots and field bindweed responses to targeted root grazing.

Yield Losses in Chickpea with Varying Densities of Dragon Spurge (Euphorbia dracunculoides)

Knowledge of economic threshold level and growth habits of weeds in chickpea is essential to implement timely, effective, and economical weed control treatments. The effect of weed density on growth and yield performance in chickpea using dragon spurge as a test weed was investigated for 2 consecutive yr. Dragon spurge density levels of 5, 10, 15, 20, 25, 30, 35, and 40 plants m−2 were compared with weed-free plots. Each increment in densities of dragon spurge from 5 to 40 plants m−2 reduced plant height, number of fruits, seeds, and dry weight per plant. Chickpea grain yield losses varied between 1 to 63% with dragon spurge density ranging from 5 to 40 plants m−2. The seed protein content of chickpea was found to be significantly reduced above a weed density of 25 plants m−2. Maximum N, P and K uptake by dragon spurge was 1,520, 1,020, and 7,350 mg m−2, respectively. Thus, dragon spurge should be controlled at densities above 5 plants m−2 to achieve optimum chickpea yield.

Long-term Trade-Offs Among Herbage Growth, Animal Production, and Supplementary Feeding in Heavily Grazed Mediterranean Grassland

A 17-yr grazing trial was conducted in the eastern Galilee of Israel to quantify trade-offs among the responses of pasture and livestock productivity, duration of grazing, amount of supplementary feed, and profitability to higher stocking density during the growing season of a Mediterranean grassland. Treatments included two stocking densities and two grazing protocols. The stocking densities throughout the grazing period were 0.55 animal unit (AU)∙ha-1, which is common in this region, and 1.1 AU∙ha-1, which is considered high. The grazing protocols were continuous grazing throughout the grazing season and split-paddock grazing in which the herd grazed one subpaddock from the onset of grazing until the pasture was depleted, after which the herd was moved to the second ungrazed subpaddock. Under both protocols, heavier stocking density reduced standing biomass of the whole paddock at the end of the growing season by 43% and grazing duration during the subsequent dry season by 38% but increased the daily consumption of supplementary feed and the weaned live-weight production per unit area. Under continuous grazing the high stocking density of 1.1 AU∙ha-1 was maintained throughout the grazing season for 17 consecutive yr with no detectable effect on productivity of the pasture, typical to the resilience of Mediterranean grasslands that have been grazed for thousands of years. The lower pasture biomass production was compensated by higher weaned calf production. At the current local prices, the heavier stocking density was close to the economically optimal stocking density for the pasture in the region. It is concluded that on Mediterranean grassland intensive use of the pasture with high stocking density during the growing season can be economically feasible in those cases where the feed requirement of the herd can be maintained throughout the growing season.

First attempt to monitor luteinizing hormone and reproductive steroids in urine samples of the Amazonian manatee (Trichechus inunguis).

The aims of this study were to validate an enzyme immunoassay (EIA) for the measurement of luteinizing hormone (LH) in urine samples of Amazonian manatees (Trichechus inunguis; Mammalia: Sirenia) and to monitor urinary LH and reproductive steroids during the ovarian cycle in this species. Urine samples were collected from two captive males following a hormonal challenge with a gonadotropin-releasing hormone (GnRH) analogue. The urinary LH results from hormonal challenge were compared with urinary androgens for the purpose of EIA validation. Furthermore, urine samples were collected daily, over a 12-wk period, from two captive adult females, for 2 consecutive yr. The urinary LH pattern from females was compared with the patterns of urinary progestagens and estrogen conjugates throughout the ovarian cycle. An LH peak was observed in both male Amazonian manatees after the hormonal challenge, occurring prior to or together with peak androgen levels. In the females, the ovarian cycle ranged from 40 to 48 days (mean of 43.7 days). Two distinct peaks of estrogen conjugates were observed across all cycles analyzed, and the urinary LH peaks observed were accompanied by peaks of urinary estrogen conjugates. The EIA was validated as a method for the quantification of urinary LH from Amazonian manatees, as it was able to detect variations in the levels of LH in urine samples. These results suggest that T. inunguis exhibits a peculiar hormonal pattern during the ovarian cycle. Therefore, further studies are desirable and necessary to clarify the relationship between this hormonal pattern and morphological changes, as well as mating behavior, in Amazonian manatee.

Pushing toward Cogongrass (Imperata cylindrica) Patch Eradication: The Influence of Herbicide Treatment and Application Timing on Cogongrass Rhizome Elimination

AbstractCogongrass, an invasive grass native to Asia, has infested thousands of hectares in the southeastern United States. Although numerous studies have examined cogongrass control, no published studies, to our knowledge, have tested strategies for cogongrass eradication. Cogongrass has a persistent, thick rhizome mat but an ephemeral seedbank; therefore, successful eradication methods must largely focus on the rhizomes. A field study to evaluate specific herbicide treatments and application timings for cogongrass patch eradication was conducted at two locations in southwestern Alabama. Herbicide treatments included glyphosate at 4.48 kg ai ha−1, imazapyr at 0.84 kg ai ha−1, and a tank mix of glyphosate and imazapyr at the same rates. Treatments were applied in May, August, or October for 3 consecutive yr, and the May glyphosate treatment included a second annual application each October. Cogongrass visual control, shoot biomass, rhizome biomass, rhizome depth, and total nonstructural carbohydrate (TNC) content were sampled during the course of the study. Cogongrass response to treatments varied by location but by 36 mo after initial treatment (MAIT), complete elimination of cogongrass shoot and rhizome biomass and 100% visual control was achieved in several herbicide treatment–timing combinations at both locations. These included glyphosate plus imazapyr at any application timing, imazapyr in August or October, and glyphosate applied in May and October each year. TNC levels of surviving healthy rhizomes were not affected by herbicide treatments, but a seasonal pattern was observed. The maximum live-rhizome depth was not influenced by any treatment, indicating that herbicides were not preferentially leaving deeper, surviving rhizomes. These results demonstrate, for the first time, that the entire rhizome layer of cogongrass can be eliminated within 3 yr with multiple treatment options and that cogongrass patch eradication is possible for many land managers.

Natural beef production and growth of Bonsmara steers stocked on rye and ryegrass pastures at humid and semiarid environments

ABSTRACT Natural, organic, and grass-fed beef provides alternative products for consumers. During 2 consecutive yr, Bonsmara steers (n = 130) grazed winter pasture at 2 environments [semiarid, Uvalde (UVL), and humid, Overton (OVT)] and received pasture only (PAS) or pasture plus 0.8% BW/steer per day corn (SUPL) to quantify growth and carcass traits. Each year, 8 steers each from UVL and OVT were slaughtered off pasture and after a 90-d feedlot (FED) period. From initiation to termination (mid-May) of stocking on Maton rye (Secale cereale L.) plus TAM-90 annual ryegrass (Lolium multiflorum Lam.) at OVT and irrigated TAM-90 at UVL, ADG was greater (P 0.05) FED ADG. Growth in BW was linear (P 0.05) by SUPL. Tenderness was enhanced (P

Effect of development system on growth and reproductive performance of beef heifers.

Reproductive performance was evaluated in beef heifers born over a 2-yr period to determine the effects of target breeding weight (TBW) and development system (SYS) on growth and subsequent reproductive efficiency. Spring-born Angus heifers (253 ± 0.7 kg) were randomly allocated over 2 consecutive yr (yr 1, n = 80; yr 2, n = 96) to be developed to either 55% (350 kg) of mature BW (moderate gain, MG) or 62% (395 kg) of mature BW (high gain, HG). Each MG and HG group was further assigned to 1 of 2 replicated systems: (1) bale graze bromegrass-alfalfa round bales in field paddocks (BG) or (2) fed bromegrass-alfalfa round bales in drylot pens (DL). Heifers were fed a diet of bromegrass-alfalfa hay (56.9% TDN; 9.8% CP) and barley grain supplement (85.1% TDN; 12.3% CP). After the 202-d development period, heifers were exposed to bulls for a 63-d breeding season. Target BW × SYS interactions were not detected for any measured parameters. During the winter development period, MG heifers had lower (P = 0.01) ADG than HG heifers and MG heifers had lighter (P = 0.01) BW at breeding. The proportion of heifers attaining puberty by 14.5 mo of age was less (P = 0.05) in MG (20 ± 4%) than HG heifers (52 ± 3%). From the end of the 202-d development period to pregnancy diagnosis, ADG was greater (P = 0.04) in MG heifers than HG heifers (0.83 vs. 0.71 kg/d). First-calf pregnancy rates were 86 and 88% for MG and HG heifers, respectively (P = 0.41). Second- and third-calf pregnancy rates of cows, developed in either a MG or HG system as heifers, were not different (P = 0.74; 94.7 vs. 95.9% and 93.8 vs. 93.9%, respectively). Economic analysis revealed a $58 reduced development cost for heifers developed to 55% compared with 62% of mature BW without a loss in reproductive performance.

The effect of bermudagrass hybrid on forage characteristics, animal performance, and grazing behavior of beef steers1,2

Bermudagrass (Cynodon dactylon [L.] Pers.) is a major feed source for ruminants across the southeastern United States. In 4 consecutive yr, 3 different bermudagrass hybrids, Alicia, Jiggs, and Tifton-85, were evaluated under a low stocking rate as forage and hay sources. The nutritive value, in situ DM digestibility, and performance and grazing behavior of beef steers under similar management were evaluated. Sampling day had an effect (P < 0.05) on all forage variables. Percentages of CP and TDN decreased while concentration of ADF, NDF, lignin, and nonfiber carbohydrates (NFC) increased as grazing season advanced. Alicia had lower nutritive value, showing greater lignin (5.3%) and indigestible fraction (44.9%) compared to Jiggs (4.9 and 35.6%, respectively) and Tifton-85 (4.5 and 40.1%, respectively). Tifton-85 contained the lowest concentration of NFC (11.8%). Steers grazing Jiggs and Tifton-85 had greater ADG (0.51 and 0.55 kg, respectively) and BW gain per hectare (258 and 279 kg, respectively) than those on Alicia (0.36 kg and 184 kg/ha, respectively); results that are probably explained by the lower nutritive value characteristics of the latter. Most grazing behavior variables were affected (P < 0.05) by time of the day (TOD) and grazing period. Two major grazing events were observed at dawn and dusk. Grazing time (32 min) was lowest (P = 0.003) while standing (140 min) and lying (98 min) time were greater (P = 0.001 and 0.04, respectively) from 1100 to 1559 h, probably as an effect of temperature and humidity at that time of the day. During summer, the temperature humidity index (THI) is above 72 (mild heat load) for the entire season and above 79 (severe heat load) during most of the daylight hours from June to August. Heat load likely affected animal performance and grazing behavior; however, some characteristics associated with these bermudagrass hybrids, especially with Alicia, such as its percentages of lignin and indigestible fraction may also partially explain the poor animal performance. In the conditions of the study, environmental variables (temperature and humidity), as well as the type of bermudagrass hybrid, affected animal performance and grazing behavior of recently weaned beef steers.

Comadia redtenbacheri(Lepidoptera: Cossidae) Pupal Development in the Laboratory

Abstract Wild populations of Comadia redtenbacheri Hammerschmidt (Lepidoptera: Cossidae), known in its larval stage as the agave redworm, are gathered intensively for sale and consumption as food. To define adequate conditions for their pupation in confinement, the effect of handling larvae during collection, their weight when induced to pupate, substrate type and the moisture content provided during this stage of development, were evaluated over 2 consecutive yr. These factors were analyzed by logistic regression (PROC GENMOD, α = 0.05), relative to the emergence of adults. The relationship between larval weight and adult sexual gender was analyzed with a contingency table. The larvae exhibited adaptation to different substrates used for pupation, and substrates could be reused. The heavy clay content soil from areas where agaves grow, mixed with vermiculite (50:50), was the most suitable substrate (estimated value 0.7304). Moisture had no significant effect on pupation. Emergence of adults was significa...

Persistent spatial patterns of recruitment in a guild of Hawaiian coral reef fishes

We herein describe a long-term (22 consecutive yr, 1990 to 2011) time series of recruitment surveys for a guild of coral-sheltering fishes at 7 to 12 sites along a 26 km section of the west coast of Hawaii Island. For arc-eye hawkfish, the numerically dominant species, recruit- ment (defined as the contribution of young-of-year to the benthic population within several months after settlement from the water column) varied 7-fold among years averaged over sites. Nonetheless, recruitment also differed spatially, with sites along the Kohala Peninsula comprising a high recruitment group. Spatial differences were persistent (some sites predictably low, others predictably high) and generally lacked demonstrable variation over time. Complementary em - pirical data suggested that post-settlement processes (predation, interspecific competition) also affected recruitment. A dispersal model combined with currents from an oceanic circulation model (Hybrid Coordinate Ocean Model, HYCOM) was used to simulate the spatial patterns of settlement by larvae released throughout the main Hawaiian Islands. These simulations generally agreed with observed spatial patterns of recruitment. Greater recruitment was also predicted near geomorphological features (headlands) on Hawaii Island most influenced by the flow of surface eddy currents generated by prevailing E-NE trade winds. Empirical data and simulations together suggest that the headlands of west Hawaii Island likely enhance recruitment by intercepting or concentrating larvae at greater rates, but that post-settlement processes dampen variations in settlement. We suggest that habitat geomorphology should be considered in the criteria used to select sites for no-take Marine Protected Areas at Hawaii Island.