Consecutive Harvests Research Articles

Six seasons of wild pea harvest in Israel: bearing on Near Eastern plant domestication

Harvest experiments of wild crop relatives are essential for our understanding of the biology of wild plants embodied in the economy of ancient forager communities. Most of the previous experimental harvest reports spanned a single season and therefore are unable to address questions concerning long-term yield potential. Herein we report on six consecutive harvest seasons taking place at four sites in Israel that harbour wild pea populations: three typical Pisum fulvum sites and one typical Pisum elatius site. Three out of the four sites showed no indication of a decline in grain yield as a result of repeated harvest and the removal of a considerable portion of mature seeds. Site-specific factors seem to have a strong influence on the grain yield as no seasonal yield similarity was observed between adjacent and ecologically similar sites harbouring the same wild pea taxon. The erratic year-to-year wild pea yield we observed calls for a reassessment of the presumed role of grain legume species in Near Eastern hunter–gatherers' diet. Combined with published data on cultivation of wild pea, our results are inconsistent with models suggesting protracted domestication of Near Eastern grain legumes or with the preliminary attempts to apply Niche Construction Theory to the study of plant domestication in the Near East.

The role of green manure nitrogen use by corn and sugarcane crops in Brazil

Three assays were developed from April 3, 1995 to October 10, 2005. The work with corn was conducted in a greenhouse, using velvet bean (Mucuna aterrima) and sunn hemp (Crotalaria juncea) as green manure with 15N labeling of either shoots or roots, in two soils with contrasting textural classes. The mineralization of N from legume plants incorporated into the two soils was investigated too. This work included two green manures: velvet bean and sunn hemp, and the common bean (Phaseolus vulgaris) residues. Nitrogen from the velvet bean accounted for a greater proportion of the soil inorganic N; shoots were responsible for most of N accumulated. Common bean residues caused immobilization of inorganic N. The leguminous species added were intensively and promptly mineralized, preserving the soil native nitrogen. One hundred days after emergence of the corn, velvet bean provided higher accumulation of nitrogen in the soil, higher absorption by corn plants and accumulation in the aerial part. The green manure decomposition was more intense in the medium textured soil. In this soil, highest nitrogen losses were also observed. The sugarcane (Saccharum spp.) was cultivated for five years in the field and was harvested three times; 15N recovery was evaluated in the first two harvests. The combination of inorganic fertilizer and green manure resulted in higher sugarcane yields than either N source applied separately; however, in the second cutting the yields were higher where sunn hemp was used than in plots with ammonium sulfate. The recovery of N by the first two consecutive harvests accounted for 19% to 21% of the N applied as sunn hemp and 46% to 49% of the N applied as ammonium sulfate. Very little inorganic N was present in the 0-40 cm soil layer with both N sources.

Biotechnological potential of non-Saccharomyces yeasts isolated during spontaneous fermentations of Malvar (Vitis vinifera cv. L.)

Non-Saccharomyces yeast species assume an important role in wine flavor. Notwithstanding, the chemical basis for the flavor characteristics of wines from some grape varieties is not yet defined. The value of this work lies in the use of Malvar white grape, an autochthonous variety from Madrid (Spain) winegrowing region to conduct spontaneous fermentations. This is the first time that a comparative characterization of a wide range of non-Saccharomyces species and a comprehensive analysis of these yeast-derived volatiles has been carried out in this grape variety. β-glucosidase and pectinase (polygalacturonase) extracellular activities were tested on agar plates as primary selection criteria among the 504 non-Saccharomyces isolated from Malvar spontaneous fermentations during four consecutive harvests. Analysis of the wines obtained after fermentation using the selected yeast strains indicates that non-Saccharomyces yeasts isolated along the fermentative process seem that could have a positive impact, showing a high variability in the volatile compounds contributing to the organoleptic characteristics of Malvar wines. Torulaspora delbrueckii CLI 918 was defined as the yeast strain with potential interest for its contribution to the aromatic wine profile with flowery and fruity aromas and could be used in mixed starter cultures with Saccharomyces cerevisiae. However, Hanseniaspora guilliermondii increased the volatile acidity and ethyl acetate, but this species along with the genus Pichia and Candida seem to provide a high quantity of extracellular enzymes which may be beneficial in wine making.

Migrant labor and wear-out in manual sugarcane harvesting in São Paulo, Brazil

The scope of this paper is to describe the work of manual sugarcane harvesters, assessing the nutritional behavior and body composition between the beginning and the end of the harvest. A descriptive longitudinal study was made of harvesters in Piracicaba, São Paulo, Brazil, who answered a socio-demographic questionnaire and authorized measurement of Body Mass Index, Body Fat Percentage and Arm Muscle Circumference at three stages. Creatine kinase on the skeletal isoform, C-reactive protein and plasma urea were measured at the end of the harvest. Thirty male migrant harvesters with ages ranging from 18 to 44 from the Northeast (Ceará) were assessed over a nine-month period. The workers suffered significant body fat and weight loss in the first half of the harvest. Eighteen workers had abnormal levels of creatine kinase and four - out of 24 who had donated blood - had altered urea levels. Sugarcane harvesting work causes weight and body fat loss and gains in the lean body mass index, which suffers wear-out when working on consecutive harvests. It can also cause changes in biochemical markers of chronic systemic inflammation. Further studies will make it possible to comprehend the relationships between stress, wear-out, labor longevity and health in sugarcane harvesting.

Preparo do solo e doses de nitrogênio em cobertura em arroz de terras altas

As influências do sistema de preparo do solo e da adubação nitrogenada não estão bem definidas para o arroz de terras altas. Assim, com o objetivo de avaliar os componentes da produtividade e o rendimento industrial do arroz de terras altas irrigado por aspersão, em função do preparo do solo e de doses de nitrogênio em cobertura, conduziu-se o experimento em condições de campo, em Selvíria (MS), por duas safras consecutivas: 2003/2004 (cultivar BRS Talento) e 2004/2005 (cultivar IAC 202). Adotou-se o delineamento em blocos casualizados, constituído pela combinação de três manejos de solo (grade aradora + grade niveladora, escarificador + grade niveladora e plantio direto) e seis doses de N em cobertura (0 kg ha-1, 25 kg ha-1, 50 kg ha-1, 75 kg ha-1, 100 kg ha-1 e 125 kg ha-1), com quatro repetições. Avaliou-se a concentração de N na folha, altura de plantas, componentes de produção, produtividade e o rendimento industrial dos grãos. O preparo do solo com escarificador + grade niveladora propiciou a obtenção de maiores produtividades das duas cultivares, enquanto a adubação nitrogenada em cobertura interferiu apenas na produtividade da cultivar IAC 202, sem afetar o rendimento industrial de grãos.

Precision techniques for improving the management of the olive groves of southern Spain

Precision agriculture has progressed in integrating different techniques, but nowadays the environmental and economic benefits of implementing the concept remain unproven. This is the first study that analyzes the agronomic and economic impact of the application of precision agriculture to a specific crop. This paper presents a study applied to olive orchards at a high level of detail. The research is conducted in order to establish a methodology for integrating Geographic Information Systems (GIS) and Global Positioning System (GPS) to implement precision agriculture in a specific olive grove in southern Spain. In this study the variability of five consecutive harvests, the homogeneous areas within the farm and the composition of the crop nutrients are all analysed. Different georeferenced management areas, analysis of cost of tillage, fertilization, herbicide treatments, pruning and harvesting are integrated into a GIS. In this study it has been possible to select a zone in the exploitation knowing immediately, for example, if the leaf N content was less than 1.5% in any campaign. The results show that detailed analyses in collecting the harvest and in the use of agrochemicals help to produce reductions in their use, with the consequent savings to olive growers and, in general, environmental benefits for the whole of society.

Impact of organic and conventional carrots on intestinal and peripheral immunity

Studies on health effects of organic (ORG) products are still limited and often contradictory. We have investigated the impact of ORG and conventional (CV) carrots from two consecutive harvest years on mouse peripheral and intestinal immunity. Danish carrots (Bolero variety) were grown in three ORG (O1, O2 and O3) and one CV cropping system (D-CV). Italian carrots (Maestro and Excelso varieties) were grown in one ORG and one CV field for each variety. Immune phenotypes of blood, spleen and intestinal lymphocytes, and cytokine serum levels were analyzed in mice fed the different carrots for 30 days. Principal component analysis (PCA) was performed in mice fed the Danish carrots. The consumption of the 'more organic' O2 and O3 carrots induced some changes in lymphocyte populations, including an increase in regulatory T cells. In Italian carrots more differences between ORG and CV were observed in the first as compared to the second year. No relevant differences were observed in cytokine secretion. PCA showed a clear separation among mice fed the O1, O2, O3 and D-CV carrots. Although a great variability was observed between the two years, an immune stimulation was found after the ORG carrot consumption.

Metabolic Profiling of a Mapping Population Exposes New Insights in the Regulation of Seed Metabolism and Seed, Fruit, and Plant Relations

To investigate the regulation of seed metabolism and to estimate the degree of metabolic natural variability, metabolite profiling and network analysis were applied to a collection of 76 different homozygous tomato introgression lines (ILs) grown in the field in two consecutive harvest seasons. Factorial ANOVA confirmed the presence of 30 metabolite quantitative trait loci (mQTL). Amino acid contents displayed a high degree of variability across the population, with similar patterns across the two seasons, while sugars exhibited significant seasonal fluctuations. Upon integration of data for tomato pericarp metabolite profiling, factorial ANOVA identified the main factor for metabolic polymorphism to be the genotypic background rather than the environment or the tissue. Analysis of the coefficient of variance indicated greater phenotypic plasticity in the ILs than in the M82 tomato cultivar. Broad-sense estimate of heritability suggested that the mode of inheritance of metabolite traits in the seed differed from that in the fruit. Correlation-based metabolic network analysis comparing metabolite data for the seed with that for the pericarp showed that the seed network displayed tighter interdependence of metabolic processes than the fruit. Amino acids in the seed metabolic network were shown to play a central hub-like role in the topology of the network, maintaining high interactions with other metabolite categories, i.e., sugars and organic acids. Network analysis identified six exceptionally highly co-regulated amino acids, Gly, Ser, Thr, Ile, Val, and Pro. The strong interdependence of this group was confirmed by the mQTL mapping. Taken together these results (i) reflect the extensive redundancy of the regulation underlying seed metabolism, (ii) demonstrate the tight co-ordination of seed metabolism with respect to fruit metabolism, and (iii) emphasize the centrality of the amino acid module in the seed metabolic network. Finally, the study highlights the added value of integrating metabolic network analysis with mQTL mapping.

Combination of sugar analysis and stable isotope ratio mass spectrometry to detect the use of artificial sugars in royal jelly production

The effects of feeding bees artificial sugars and/or proteins on the sugar compositions and (13)C isotopic measurements of royal jellies (RJs) were evaluated. The sugars fed to the bees were two C4 sugars (cane sugar and maize hydrolysate), two C3 sugars (sugar beet, cereal starch hydrolysate), and honey. The proteins fed to them were pollen, soybean, and yeast powder proteins. To evaluate the influence of the sugar and/or protein feeding over time, samples were collected during six consecutive harvests. (13)C isotopic ratio measurements of natural RJs gave values of around -25 ‰, which were also seen for RJs obtained when the bees were fed honey or C3 sugars. However, the RJs obtained when the bees were fed cane sugar or corn hydrolysate (regardless of whether they were also fed proteins) gave values of up to -17 ‰. Sugar content analysis revealed that the composition of maltose, maltotriose, sucrose, and erlose varied significantly over time in accordance with the composition of the syrup fed to the bees. When corn and cereal starch hydrolysates were fed to the bees, the maltose and maltotriose contents of the RJs increased up to 5.0 and 1.3 %, respectively, compared to the levels seen in authentic samples (i.e., samples obtained when the bees were fed natural food: honey and pollen) that were inferior to 0.2% and not detected, respectively. The sucrose and erlose contents of natural RJs were around 0.2 %, whereas those in RJs obtained when the bees were fed cane or beet sugar were as much as 4.0 and 1.3 %, respectively. The combination of sugar analysis and (13)C isotopic ratio measurements represents a very efficient analytical methodology for detecting (from early harvests onward) the use of C4 and C3 artificial sugars in the production of RJ.

A comparative study on phenolic composition, antioxidant activity and essential oil content of wild and cultivated sage (Salvia fruticosa Miller) as influenced by storage

In the present study, the main quality parameters such as moisture content, extract yields, total essential oils, phenolic content and composition, and antioxidant activity of wild and cultivated Salvia fruticosa were elaborately investigated in six-month storage period for two consecutive years. The cultivated samples had higher total phenolic content than those of the wild samples. On the contrary, wild samples had higher extract yields, total flavonoids and total essential oils. Seventeen different phenolic compounds, composed of seven phenolic acids and ten flavonoids, were identified and quantified in both cultivated and wild S. fruticosa. Rosmarinic, p-coumaric and caffeic acids were determined to be the principal phenolic acids. The major flavonoids however were myricetin, morin and luteolin. The phenolic components of vanillic acid, gallic acid, chlorogenic acid, p-coumaric acid, myricetin and morin increased, whereas caffeic acid, catechin, epicatechin, luteolin and apigenin decreased by cultivation. The phenolic components were found to be either lower or not changed in consecutive harvesting year. They also did not significantly change during the storage period. The antioxidant activity of the samples was found to be higher for the second harvesting year whereas there were no significant differences between wild and cultivated S. fruticosa during storage period.

Heavy metal phytoextraction by Sedum alfredii is affected by continual clipping and phosphorus fertilization amendment

Improving the efficacy of phytoextraction is critical for its successful application in metal contaminated soils. Mineral nutrition affects plant growth and metal absorption and subsequently the accumulation of heavy metal through hyper-accumulator plants. This study assessed the effects of di-hydrogen phosphates (KH2PO4, Ca(H2PO4)2, NaH2PO4 and NH4H2PO4) application at three levels (22, 88 and 352 mg P/kg soil) on Sedum alfredii growth and metal uptake by three consecutive harvests on aged and Zn/Cd combined contaminated paddy soil. The addition of phosphates (P) significantly increased the amount of Zn taken up by S. alfredii due to increased shoot Zn concentration and dry matter yield (DMY) (P < 0.05). The highest phytoextraction of Zn and Cd was observed in KH2PO4 and NH4H2PO4 treatment at 352 mg P/kg soil. The amount of Zn removed by phytoextraction increased in the order of 1st clipping < 2nd clipping < 3rd clipping, and for Cd extraction the order was 2nd clipping < 1st clipping < 3rd clipping. These results indicate that the application of P fertilizers coupled with multiple cuttings can enhance the removal of Zn and Cd from contaminated soils by S. alfredii, thus shortening the time needed for accomplishing remediation goals.

A mixed model QTL analysis for sugarcane multiple-harvest-location trial data

Sugarcane-breeding programs take at least 12 years to develop new commercial cultivars. Molecular markers offer a possibility to study the genetic architecture of quantitative traits in sugarcane, and they may be used in marker-assisted selection to speed up artificial selection. Although the performance of sugarcane progenies in breeding programs are commonly evaluated across a range of locations and harvest years, many of the QTL detection methods ignore two- and three-way interactions between QTL, harvest, and location. In this work, a strategy for QTL detection in multi-harvest-location trial data, based on interval mapping and mixed models, is proposed and applied to map QTL effects on a segregating progeny from a biparental cross of pre-commercial Brazilian cultivars, evaluated at two locations and three consecutive harvest years for cane yield (tonnes per hectare), sugar yield (tonnes per hectare), fiber percent, and sucrose content. In the mixed model, we have included appropriate (co)variance structures for modeling heterogeneity and correlation of genetic effects and non-genetic residual effects. Forty-six QTLs were found: 13 QTLs for cane yield, 14 for sugar yield, 11 for fiber percent, and 8 for sucrose content. In addition, QTL by harvest, QTL by location, and QTL by harvest by location interaction effects were significant for all evaluated traits (30 QTLs showed some interaction, and 16 none). Our results contribute to a better understanding of the genetic architecture of complex traits related to biomass production and sucrose content in sugarcane.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-011-1748-8) contains supplementary material, which is available to authorized users.

Possibilities of increasing the residual water‐soluble carbohydrate concentration and aerobic stability of low dry‐matter perennial ryegrass silage through additive and cultivar use

AbstractIncreasing the residual water‐soluble carbohydrate (WSC) concentration in silage may improve the nutritional value but impair aerobic stability. Our aim was to determine whether the residual WSC concentration and aerobic stability of low dry‐matter (&lt;135 g kg−1) perennial ryegrass silage could be manipulated through the judicious use of additive and cultivar. Seven additive treatments, including three innovative treatments, were compared across four consecutive harvests of the cultivars AberDart (bred to accumulate high concentrations of herbage WSC) and Fennema (control). The standard of fermentation of silage ensiled without additive (untreated) ranged from very bad to excellent. Application of ammonium tetraformate, at 3 and 6 L t−1, or homofermentative lactic acid bacteria (LAB) alone had an inconsistent effect on the fermentation and aerobic stability, and negligible effect on residual WSC concentration. A mixture of Lactobacillus buchneri and homofermentative LAB was not an effective silage additive, producing generally poorly fermented silage. An antimicrobial mixture of sodium benzoate, sodium propionate, sodium nitrite and hexamethylenetetramine, applied at 2·5 and 5 L t−1, frequently improved the standard of fermentation, but the effects were subject to the application rate. The high application rate was the most effective additive evaluated at improving the fermentation and increasing residual WSC concentration and consistently produced silage of excellent standard of fermentation. However, the antimicrobial mixture was not effective at protecting against aerobic instability. The effects of additive treatment were largely inconsistent across cultivars. Overall, AberDart had a negligible effect on the silage fermentation, residual WSC concentration and aerobic stability compared with Fennema.

Nutrients and antinutrients composition of raw, cooked and sun-dried sweet potato leaves

Traditional indigenous vegetables are the most economically efficient source of micronutrients in terms of both land required and production costs per unit. Promotion of production and consumption of such micronutrient-rich foods will improve intakes, the overall diet, and health status. This study aimed to determine nutrient (iron, calcium, vitamin A and ascorbic acid) and anti-nutrient (oxalates and polyphenols) contents in raw, cooked and dried sweet potato leaves Two varieties of sweet potatoes, which were identified as commonly grown for leaves consumption were analyzed at Department of Food Technology, Sokoine University of Agriculture and at the Government Chief Chemist Laboratory Tanzania. The analysis included proximate, nutrient (ascorbic acid, carotenoids, iron and calcium) and anti-nutrient (oxalate and polyphenols) composition. The purple midrib sweet potato leaves were further analyzed for nutrient and anti-nutrient retention after cooking (with and without lemon) and open sun-drying (with and without salting). There was no significant difference (P>0.05) between the two varieties in crude protein, crude lipid and moisture content. The purple midrib sweet potato leaves had significantly (P<0.05) higher ash, crude fibre, carotenoids, calcium and iron contents while the green midrib sweet potato leaves had significantly (P<0.05) higher ascorbic acid content. The polyphenols were about 4 times higher in the purple midrib sweet potato leaves (22.16%) as compared to that of the green ones (5.28%), which had significantly higher oxalate levels (3730 mg/100g). Drying with salt and cooking with lemon reduced polyphenols significantly (p<0.05), with retention of 42% and 56% respectively; while cooking with lemon lowered significantly the oxalate levels. The traditional methods of cooking SPL with addition of lemon is advantageous because it reduces polyphenols while retaining higher levels of minerals, β carotene and vitamin C. Drying with salt results in a nutritionally and organoleptically good product, hence, drying with salt and cooking with addition of lemon is encouraged. Since the sweet potato leaves are harvested more than once before the plant is uprooted, further studies are recommended to assess whether there is variation in nutrient and anti-nutrient contents in consecutive harvests. Key words: Sweet potato leaves, nutrients, antinutrients

Bioavailable Phosphorus in Animal Waste Amended Soils: Using Actual Crop Uptake and P Mass Balance Approach

Animal manure amended soils often contain large amounts of bioavailable phosphorus (P) and constitute high risk for the deterioration of surface water quality through eutrophication. Current standards set for the safe disposal of animal manure through soil application are based on the assumption that phosphorus in all P sources would behave similarly. The primary objective of this study was to understand the influence of P from several manure and mineral fertilizer sources applied at 0, 50, and 150 mg P kg(-1) on two measures of bioavailable P to six soils of different initial soil test P levels using corn (Zea mays L.) P uptake and an iron oxide strip method for soil analysis (FeO-P). Total net bioavailable P (TNBP) was calculated by subtracting total P uptake by corn after seven consecutive harvests in control treatments that did not receive P from the P uptake from P-amended treatments. Net biovavailable P after the first harvest (NBP1) was calculated in a similar fashion but only using data from the first harvest. Significant differences in TNBP and NBP1 were found when comparing P sources. The hog (Sus scrofa) manure had the greatest P bioavailability while turkey (Meleagris gallopava) litter had the lowest among the animal P sources across all soils and levels of P application. Significant differences were also found between soils with the highest amounts of TNBP and NBP1 found in the Woodson soil and lowest detected in the Crete soil for most P sources. The FeO-P method was useful in predicting TNBP from most P sources.

The carbon implications of large‐scale afforestation of agriculturally marginal land with short‐rotation willow in Saskatchewan

AbstractAfforestation with short‐rotation coppice (SRC) willow plantations for the purpose of producing bioenergy feedstock was contemplated as one potential climate change mitigation option. The objectives of this study were to assess the magnitude of this mitigation potential by addressing: (i) the land area potentially available for SRC systems in the province of Saskatchewan, Canada; (ii) the potential biomass yields of SRC plantations; and (iii) the carbon implications from such a large‐scale afforestation program. Digital soils and land‐use data were used to identify, map, and group into clusters of similar polygons 2.12 million hectares (Mha) of agriculturally marginal land that was potentially suitable for willow in the Boreal Plains and Prairies ecozones in Saskatchewan. The Physiological Principles in Predicting Growth (3PG) model was calibrated with data from SRC experiments in Saskatchewan, to quantify potential willow biomass yields, and the Carbon Budget Model of the Canadian Forest Sector (CBM‐CFS3), was used to simulate stand and landscape‐level C fluxes and stocks. Short‐rotation willow plantations managed in 3 year rotations for seven consecutive harvests (21 years) after coppicing at Year 1 produced about 12 Mg ha−1 yr−1 biomass. The more significant contribution to the C cycle was the cumulative harvest. After 44 years, the potential average cumulative harvested biomass C in the Prairies was 244 Mg C ha−1 (5.5 Mg C ha−1 yr−1) about 20% higher than the average for the Boreal Plains, 203 Mg C ha−1 (4.6 Mg C ha−1 yr−1). This analysis did not consider afforestation costs, rate of establishment of willow plantations, and other constraints, such as drought and disease effects on biomass yield. The results must therefore be interpreted as a biophysical mitigation potential with the technical and economic potential being both lower than our estimates. Nevertheless, short‐rotation bioenergy plantations offer one potential mitigation option to reduce the rate of CO2 accumulation in the earth's atmosphere and further research is needed to operationalise such a mitigation effort.

15N-labeled nitrogen from green manure and ammonium sulfate utilization by the sugarcane ratoon

Legumes as green manure are alternative sources of nitrogen (N) for crops and can supplement or even replace mineral nitrogen fertilization due to their potential for biological nitrogen fixation (BNF). The utilization of nitrogen by sugarcane (Saccharum spp.) fertilized with sunn hemp (Crotalaria juncea L.) and ammonium sulfate (AS) was evaluated using the 15N tracer technique. N was added at the rate of 196 and 70 kg ha-1 as 15Nlabeled sunn hemp green manure (SH) and as ammonium sulfate (AS), respectively. Treatments were: (i) Control; (ii) AS15N; (iii) SH15N + AS; (iv) SH15N; and (v) AS15N + SH. Sugarcane was cultivated for five years and was harvested three times. 15N recovery was evaluated in the two first harvests. In the sum of the three harvests, the highest stalk yields were obtained with a combination of green manure and inorganic N fertilizer; however, in the second cutting the yields were higher where SH was used than in plots with AS. The recovery of N by the first two consecutive harvests accounted for 19 to 21% of the N applied as leguminous green manure and 46 to 49% of the N applied as AS. The amounts of inorganic N, derived from both N sources, present in the 0-0.4 m layer of soil in the first season after N application and were below 1 kg ha-1.

Efficiency of green waste compost and biochar soil amendments for reducing lead and copper mobility and uptake to ryegrass

Green waste compost and biochar amendments were assessed for their assistance in regulating the mobility of copper (Cu) and lead (Pb) and the resultant uptake of these metals into vegetation. The amendments were mixed with a heavily Cu and Pb contaminated soil (600 and 21,000 mg kg −1, respectively) from a former copper mine in Cheshire (UK), on a volume basis both singly and in combination in greenhouse pot trials. Ryegrass ( Lolium perenne L. var. Cadix) was grown for the following 4 months during which biomass, metals in soil pore water and plant uptake were measured in three consecutive harvests. Very high Pb concentrations in pore water from untreated soil (>80 mg l −1) were reduced furthest by compost amendment (<5 mg l −1) whereas biochar was the more effective treatment at reducing pore water Cu concentrations. Duly, ryegrass shoot Cu levels were reduced and large, significant reductions in shoot Pb levels were observed after biochar and compost amendments, respectively during successive harvests. However, because green waste compost singly and in combination with biochar vividly enhanced biomass yields, harvestable amounts of Pb were only significantly reduced by the compost amendment which had reduced shoot Pb levels furthest. The low biomass of ryegrass with biochar amendment meant that this was the only amendment which did not significantly increase harvestable amounts of Cu. Therefore the two amendments have opposing metal specific suitability for treating this contaminated soil regarding whether it is a maximum reduction in plant tissue metal concentration or a maximum reduction in harvestable amount of metal that is required.

The 1738–41 Harvest Crisis in Scotland

Between 1738 and 1741 Scotland experienced one of the harshest harvest crises and depressions in the eighteenth-century. After at least two consecutive harvest failures (in 1739 and 1740 and perhaps also in 1738) agrarian and industrial output contracted, the price level doubled, and average incomes fell below subsistence. Due to an increase in mortality, there was also a considerable contraction in aggregate demand. Data drawn from both the micro- as well as the macro-level shows the disastrous economic impact such deficient harvests – the depression's initial trigger – would have upon Scotland, a pre-industrial economy dominated by agriculture. Such shocks in agrarian supply tended to work out as general adverse shocks in aggregate supply, as the economy's business cycle was to a large extent determined by movements in the harvest cycle. The implicit task of the paper also is to point out the variety of available sources for, as well as one possible strategy of, writing a quantitative macro-economic history of eighteenth-century Scotland.

Phenolic Profile and Hydrophilic Antioxidant Capacity as Chemotaxonomic Markers of Tomato Varieties

Tomatoes (Solanum lycopersicum L.), the second most important vegetable crop worldwide, are a key component in the so-called "Mediterranean diet", which is strongly associated with a reduced risk of chronic degenerative diseases. In this work, we evaluate the differences in the total and individual polyphenol content and hydrophilic antioxidant capacity of seven varieties of tomato cultivated in Vegas Bajas del Guadiana, Badajoz (Spain), which were collected from two consecutive harvests (2008-2009). Hydrophilic antioxidant capacity was evaluated using the TEAC assay, while the Folin-Ciocalteau assay with a previous cleanup was used to establish total polyphenol content. The method was optimized and validated. Individual polyphenols were quantified using liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) on a triple quadrupole. All compounds were found to be significantly different when analysis of variance was performed. Results from the principal component analysis show that phenolic compounds and hydrophilic antioxidant capacity were responsible for the differences among tomato samples according to variety.