Conifer Pollen Research Articles

Modern Abies, Picea, and Tsuga pollen distribution along the elevation gradient in East Asia

Sub-alpine dark coniferous forests (SCFs) exhibit distinct latitudinal and longitudinal elevation distributions in East Asia. The SCF genera Abies, Picea, and Tsuga produce distinctive pollen and have specific elevation and climate requirements, allowing for the reconstruction of paleo-climatic and paleo-elevational changes from fossil pollen sequences. However, the relationship between SCF elevational distribution and the percentage of coniferous pollen has not been elucidated at the continental scale. In this study, we determined the relationship between Abies, Picea, and Tsuga pollen percentages and SCF elevation distributions in East Asia using an integrated pollen dataset comprising 4863 modern samples. The total percentage of these pollen types showed bimodal patterns with elevation and unimodal patterns with climate. Maximum pollen percentages occurred at elevations of 0–1000 and 2000–4600 m, mean annual temperatures of −5–10 ℃, and mean annual precipitation values of 100–1500 mm. The elevation with the highest proportion of Abies + Picea + Tsuga pollen increased with decreasing latitude: from 500 m (>43°N) to 2300 m (37–43°N), to 3500 m (30–37°N), and to 4000 m (18–30°N). Elevation also exhibited non-monotonic variations with longitude, first increasing from 2500 m (<93°E) to 3500 m (93–102°E), and then decreased to 2500 m (102–113°E) and 500 m (>113°E). The spatial patterns of Abies, Picea, and Tsuga pollen abundance were closely correlated with modern SCF distributions, which are largely modulated by climate and elevation. This study lays the foundation for reconstructing paleo-climate and paleo-elevation variations across East Asia.

Pollen evidence of enhanced Yellow River provenance into the South Yellow Sea after the Middle Pleistocene Transition

Quaternary sediment of the South Yellow Sea (SYS) is usually assumed to record climatic changes, tectonic activities and major river evolutions. However, as an important terrigenous indicator, a long sequence of pollen proxy in the SYS lacks for studies of river provenance and climate changes. In this study, we focused on a 150-m pollen record in the SYS that covered most of the Quaternary period over the past 1.8 Ma. The most significant changes of pollen assemblage were caused by river provenance, that high pollen percentages of broadleaved trees such as Castanea/Castanopsis reflect a dominant provenance of the Yangtze River since ca. 1.8 Ma, with a decline trend during the Mid-Pleistocene transition (MPT) mainly between 1.1 and 0.8 Ma. The sharp increase in coniferous pollen, particularly Picea, originated from the Yellow River drainage areas, suggesting a major alteration in terrigenous pollen source from the Yangtze River to the Yellow River at ∼0.8 Ma, which agrees well with the timing of the final integration of the modern Yellow River. The high pollen quantities of Artemisia and Ephedra also shows the enhanced Yellow River provenance where most landscape was covered by semi-arid open vegetation (e.g. steppe). The alternate appearances of marine foraminifera and dinoflagellate cysts, and freshwater wetland plants (Cyperaceae, Poaceae) through the interglacial intervals from MIS 19/21 demonstrate different estuary sedimentary environments and diverse salinity, associated with the sea-level fluctuations. The pollen concentration suggests an increase in terrigenous materials towards the interglacial MIS 11 and the preliminary delta formation of the Yellow River. We propose that the terrigenous input from the Yellow River accelerated after the MPT, and combined effects of tectonic movement drove the evolution of the river delta plains in eastern China. Our data also reveal some secondary periodic changes in evergreen broadleaved forest during the past 1.8 Ma and cold-tolerant coniferous forest since particularly the MPT, which are generally linked with the 400-ka long eccentricity cycle and the variability of monsoon climate.

Temperature dependence of pollen germination and tube growth in conifers relates to their distribution along an elevational gradient in Washington State, USA.

Pollen germination and tube growth are essential processes for successful fertilization. They are among the most temperature-vulnerable stages and subsequently affect seed production and determine population persistence and species distribution under climate change. Our study aims to investigate intra- and inter-specific variations in the temperature dependence of pollen germination and tube length growth and to explore how these variations differ for pollen from elevational gradients. We focused on three conifer species, Pinus contorta, Picea engelmannii, and Pinus ponderosa, with pollen collected from 350 to 2200m elevation in Washington State, USA. We conducted pollen viability tests at temperatures from 5 to 40°C in 5°C intervals. After testing for four days, we took images of these samples under a microscope to monitor pollen germination percentage (GP) and tube length (TL). We applied the Gamma function to describe the temperature dependence of GP and TL and estimated key parameters, including the optimal temperature for GP (Topt_GP) and TL (Topt_TL). Results showed that pollen from three species and different elevations within a species have different GP, TL, Topt_GP, and Topt_TL. The population with a higher Topt_GP would also have a higher Topt_TL, while Topt_TL was generally higher than Topt_GP, i.e., a positive but not one-to-one relationship. However, only Pinus contorta showed that populations from higher elevations have lower Topt_GP and Topt_TL and vice versa. The variability in GP increased at extreme temperatures, whereas the variability in TL was greatest near Topt_TL. Our study demonstrates the temperature dependences of three conifers across a wide range of temperatures. Pollen germination and tube growth are highly sensitive to temperature conditions and vary among species and elevations, affecting their reproduction success during warming. Our findings can provide valuable insights to advance our understanding of how conifer pollen responds to rising temperatures.

Pollen in Polar Ice Implies Eastern Canadian Forest Dynamics Diverged From Climate After European Settlement

AbstractRapid warming and human exploitation threaten boreal forests. Understanding links among vegetation, climate, and people in this vast biome requires highly resolved long‐term records that integrate regional inputs. We developed an 850‐year pollen‐based record of supraregional vegetation change using a southern Greenland ice core and atmospheric modeling that identified the boreal and mixed‐conifer forests of eastern Canada as the dominant pollen source regions. Conifer pollen increased ∼1400 CE at the onset of the cooler and drier Little Ice Age. A subsequent decline began ∼1650 CE and a statistically significant pollen change after 1760 CE suggests ecological consequences of the Little Ice Age cooling and initial human exploitation that persisted until recent decades. These supraregional changes are broadly consistent with local records and demonstrate intensification of human impacts on northern forests, suggesting a shift from a climate‐modulated to an increasingly human‐controlled system during recent centuries.

Late Quaternary Dynamics of Landscape and Climate in the North of the West Siberian Plain Revealed by Paleoecological Studies of Peat and Lake Sediments

West Siberian mires covering more than 50% of area in the subarctic are still poorly investigated despite their thick peat sediments suitable for paleogeographic research of past long-term landscape and climatic changes. In this research, a combination of paleoecological methods were used, including the analysis of pollen, spores, diatoms, NPPs, and macrofossils, the measurement of peat humification, and quantitative paleoclimate reconstruction. This multi-proxy approach was applied to study a palsa bog (frost peat heave mound) located in the north of western Siberia on the border of the northern taiga and forest–tundra (65°18′56″ N, 72°52′27″ E). Chronology is based on 21 radiocarbon dates, which were calibrated in CLAM. Studies have shown that sediments of palsa bog Nadym of a 1050 cm thickness were formed both in the Holocene and earlier periods of the Quaternary. Radiocarbon dating worked well for peat sequences (610 cm thick), but failed for underlying lacustrine and mineral sediments (440 cm thick). Numerous remains of salt-water diatoms and exotic Neogene pollen were found in the lacustrine sediments (650–850 cm). The oldest sediments (850–1050 cm) have signs of secondary epicryogenic diagenesis in the form of cryogenic iron-enriched granules. Both lacustrine and bottom sediments contain abundant coniferous pollen. At the same time, spore–pollen complexes dated to the Last Glacial Age were not found in low sediments because of failed dates. To explain this, the authors turn to the hypothesis of glyacioisostatic compensation, according to which the study area was uplifted during the Last Glacial Age and the ancient deposits underwent secondary diagenesis in subaerial conditions. Holocene lacustrine sedimentation began to form about 9800 cal. a BP. These lacustrine sediments turned out to be enriched in redeposited Neogene pollen and diatoms. It was interpreted as an influence of excess humid climate in combination with geological subsidence of landscape in the study area during the Early Holocene. This caused lake formation and introduction of exotic microfossils via surface run-off from higher-relief areas in the catchment. Syngenetic sedimentation in the Nadym section is associated only with peat-mire deposits covering the last 8400 cal. a BP. For this time, the dynamic of vegetation cover and quantitative changes in paleoclimate were reconstructed using spore–pollen, macrofossil, humus, and NPP data as well as the information–statistical method of V.A. Klimanov. The spore–pollen analysis revealed four main phases in the development of vegetation cover: 1. Spruce–birch forests with open meadows and lakes (8400–7600 cal. a BP); 2. Dominance of spruce forests and thawed eutrophic (minerotrophic) mires (7600 to 6500 cal. a BP); 3. Coniferous–birch forests and thawed mesotrophic mires (6500 to 4500 cal. a BP); 4. Birch–pine forests and oligotrophic (ombrotrophic) bogs with permafrost mounds—palsa bogs (approx. the last 4500 years). Quantitative reconstructions of paleoclimate based on pollen data show that in most cases the periods of a sharp decrease in mean January and mean July temperatures coincided with episodes of low solar activity. The assumption was made about the determining influence of solar activity on the formation of permafrost in the soils and mires of the study area. Sun minima caused permafrost formation in the mire periodically since 8400 cal. a BP in study peatland, but complete freezing of the peat mire and formation of the palsa bog occurred at c. 2800 cal. a BP.

Palynology of the Upper Gondwana deposits from the Chintalapudi Sub-basin, South India: Insights into age and palaeodepositional settings

Palynological data were analyzed to determine the age and depositional settings of the Jangareddygudem area in Godavari Graben, South India. The abundance of coniferous pollen grains (Araucariacites spp., Podocarpidites spp., and Callialasporites spp.) as well as pteridophytic and bryophytic trilete spore characterizes the palynofloral assemblage. The First Occurrences (FOs) of biostratigraphically significant palynomorphs taxa have been identified, which allowed us to assign the studied stratigraphic sequence to the upper Aptian–lower Albian. Recovered palynoassemblages are compared with other contemporaneous assemblages from different Gondwana continents. Palynofacies research supports hetero-lithic shelf (proximal shelf) rock formation with gradual and continuous sediment input under dysoxic to anoxic conditions. The recovered palynomorphs consistently suggest cool and humid climates during rock deposition, favoring the formation and development of a dense canopy of luxurious coniferous forests near the coast.

Rapid reorganization of the Pearl River network driven by spreading of the South China Sea at around 32 Ma

Provenance analyses on sediments from depositional sinks have provided valuable information about the birth of the Pearl River, a key event in the landscape history of South China. However, uncertainty remains due to the difficulty in clearly identifying the source regions by geochemical or geochronological approaches, giving rise to disputes on the driving mechanisms underlying river network evolution. Here we combined pollen and grain-size analyses of terrigenous sediments from International Ocean Discovery Program site U1501 to further constrain the timing and pattern of the Pearl River network evolution during the late Eocene and Oligocene. The results reveal a sharp, synchronous transition at ∼32 Ma in both proxies. The sudden occurrence of the rainforest coniferous pollen Dacrydium and increased abundances of the alpine coniferous pollen Abies/Picea indicate a new mountainous source region stretching across the tropical-subtropical ecotone after 31.5 Ma. The simultaneous input of a distinct coarse fraction of siliciclastic components implied that the terrigenous sediments were transported by a large, regional-scale river, in addition to the preceding small rivers draining local uplifts proximal to the site. The formation of a proto-Pearl River originating from the Cathaysian mountains and flowing through the rift basins of the continental margin is thus proposed. Moreover, when integrated with co-located calcareous nannofossil records indicative of a synchronous deepening process of the sea basin, our results link this early river network reorganization event to the spreading of the South China Sea rather than to the uplift of the Tibetan Plateau.

Palynological implications for the paleoclimate and paleoceanographic reconstruction of the East Sea since the early Pleistocene at IODP site U1430

Palynomorphs, including pollen and dinoflagellate cysts from marine sediments, can provide a history of paleoclimate and paleoceanography. We present new palynological profiles based on a core from the Integrated Ocean Drilling Program Site U1430, which was drilled in the Ulleung Plateau East Sea. This core covers the past 2 Ma and spans the Mid-Pleistocene Transition (MPT, 1.2–0.8 Ma) and Mid-Brunhes Event (MBE, ∼0.43 Ma). Terrestrial and marine palynomorph records indicate orbital-scale variations in paleoclimate and paleoceanography, reflecting changes in the interconnections between vegetation, climate, and the ocean. Before the MPT, the high pollen taxa ratio and abundant temperate pollen suggest a relatively warm temperature associated with astronomical forcing-induced insolation changes. At ca. 1.3 Ma, boreal coniferous pollen increased, suggesting intensification of the East Asian winter monsoon (EAWM). The long-term trend of boreal coniferous pollen concentration was similar to that of benthic δ18O. This orbital scale boreal conifer pollen assemblage variation indicates Quaternary EAWM evolution controlled by changes in global ice volume and climatic cooling. The marine palynological data reflect the ocean circulation history of the study area, where sea level fluctuations and tectonic activity played important roles around the marginal sea. The abundance of warm-water dinoflagellate species after ca. 1.9 Ma, including Tuberculodinium vancampoae, indicates Tsushima Warm Current intrusion related to the expansion of the Korea (Tsushima) Strait. Nematosphaeropsis labyrinthus and other cold-water species increased markedly during the MPT, suggesting a low sea surface temperature and weakened vertical mixing of the East Sea due to the cooled climate and sea ice during the MPT. The marked sea level fluctuation associated with the glacial–interglacial cycling after the MBE resulted in an assemblage composition shift from cold to warm water species. Tsuga and Betula thrived during mild winters, primarily influenced by winter insolation. These genera were particularly prosperous in the early Pleistocene, coinciding with a strong intrusion of the Tsushima Warm Current (TWC). Our findings should be considered in developing a comprehensive understanding of the paleoclimate and paleoceanographic history of northern East Asia across the major climate transitions of the Pleistocene.

Paleoenvironments and stratigraphy of the Valanginian–Hauterivian carbonates of the Arabian Platform: Implications for the recognition of the Weissert Event on the Southern Tethys

On the Arabian Platform, the Valanginian Weissert Event is associated with the sudden drowning of a major north-easterly prograding carbonate platform located on the southern margin of the Tethys Ocean. This was associated with a sharp back-stepping of the main facies belts to the south-west, bringing shallow marine carbonates into the previously restricted platform top. Integrated detailed facies analysis, biostratigraphy (including nannofossil and palynology) and carbon isotopes have been used to reconstruct the evolution of the carbonate shelf during the Valanginian–Hauterivian in Saudi Arabia. During the early Valanginian the study area was dominated by the deposition of dolomitized clay-prone microbial intertidal mudstones, which passed basinwards into low-energy inner ramp limestones. These facies encompass the initial stage of a 3rd order transgressive trend above the regional Lower Valanginian Unconformity. They are sharply overlain by Lithocodium/Bacinella-dominated floatstones, indicating the maximum flooding of the platform during the late Valanginian. These deposits systematically increase in thickness and abundance towards the platform margin and are associated with a broad positive excursion in δ13C (c.+2‰ V-PDB), corresponding to the Weissert Event. The presence of abundant Lithocodium/Bacinella in strata deposited during the major flooding of the platform is consistent with higher nutrient levels and increased upwelling during the late Valanginian. The Valanginian–Hauterivian boundary is marked by a regional unconformity identified through palynology and benthic foraminifera assemblages, and locally confirmed by carbon isotopes. The combination of carbon isotope data with detailed palynology and micropaleontology provided a stratigraphic resolution in line with ammonite biozones published in the western Tethys. The Valanginian–Hauterivian continental palynoflora, described for the first time on the Arabian Platform, is dominated by primitive conifer pollen such as Classopollis suggesting a rather arid climate in agreement with published reconstructions. In addition, the occurrence of various fern and bryophyte spores suggests an increase in humidity along coastline areas, associated with higher continental input into inner ramp settings, therefore affecting the carbonate factory.

Biodiversity of ecosystems in an arid setting: The late Albian plant communities and associated biota from eastern Iberia.

Deserts are stressful environments where the living beings must acquire different strategies to survive due to the water stress conditions. From the late Albian to the early Cenomanian, the northern and eastern parts of Iberia were the location of the desert system represented by deposits assigned to the Utrillas Group, which bear abundant amber with numerous bioinclusions, including diverse arthropods and vertebrate remains. In the Maestrazgo Basin (E Spain), the late Albian to early Cenomanian sedimentary succession represents the most distal part of the desert system (fore-erg) that was characterised by an alternation of aeolian and shallow marine sedimentary environments in the proximity of the Western Tethys palaeo-coast, with rare to frequent dinoflagellate cysts. The terrestrial ecosystems from this area were biodiverse, and comprised plant communities whose fossils are associated with sedimentological indicators of aridity. The palynoflora dominated by wind-transported conifer pollen is interpreted to reflect various types of xerophytic woodlands from the hinterlands and the coastal settings. Therefore, fern and angiosperm communities abundantly grew in wet interdunes and coastal wetlands (temporary to semi-permanent freshwater/salt marshes and water bodies). In addition, the occurrence of low-diversity megafloral assemblages reflects the existence of coastal salt-influenced settings. The palaeobotanical study carried out in this paper which is an integrative work on palynology and palaeobotany, does not only allow the reconstruction of the vegetation that developed in the mid-Cretaceous fore-erg from the eastern Iberia, in addition, provides new biostratigraphic and palaeogeographic data considering the context of angiosperm radiation as well as the biota inferred in the amber-bearing outcrops of San Just, Arroyo de la Pascueta and La Hoya (within Cortes de Arenoso succesion). Importantly, the studied assemblages include Afropollis, Dichastopollenites, Cretacaeiporites together with pollen produced by Ephedraceae (known for its tolerance to arid conditions). The presence of these pollen grains, typical for northern Gondwana, associates the Iberian ecosystems with those characterising the mentioned region.

Plant Megafossils, Palynomorphs, and Paleoenvironment from the Late Middle to Late Eocene Burnaby Mountain Flora, Huntingdon Formation, British Columbia, Canada

Premise of research. The Eocene fossil flora of the area around Vancouver, British Columbia, is poorly known despite work beginning in the 1890s to 1920s. The floristic character of the previously unstudied Burnaby Mountain flora from the Huntingdon Formation in British Columbia is reconstructed using plant megafossils and palynology. This site offers insight into the terrestrial vegetation and paleoclimate during the late middle to late Eocene of the Pacific Northwest of North America in a coastal setting during a global cooling trend.Methodology. Megaflora and microflora were identified, and the combined flora was compared with that of coeval floras from northwestern Washington. Paleoclimate was reconstructed from leaf morphology using the Climate Leaf Analysis Multivariate Program, leaf margin analysis, and leaf area analysis. A probabilistic nearest-living-relative approach was used to reconstruct paleoclimate independently of leaf morphology, using taxonomic identifications from both mega- and microfossils. These data were combined in an ensemble approach.Pivotal results. The Burnaby Mountain fossil flora is late middle Eocene to late Eocene in age and shares key plant taxa with the coeval Upper Ravenian flora of the Puget Group and the upper Chumstick Formation of northwestern Washington. The fossil flora contained a mix of subtropical and temperate forest elements, including rare palm and possible cycad leaf fragments, rare conifer pollen, and a diversity of broad-leaved trees.Conclusions. The reconstructed paleoclimate suggests humid warm-temperate to marginally subtropical conditions in coastal British Columbia during the late middle Eocene to late Eocene. An ensemble paleoclimate approach provided a most parsimonious mean annual temperature estimate of 16.2°C±3.1°C for the Burnaby Mountain fossils and mean annual precipitation of 134±56 cm. A modern climatic analog is present on the East Coast of the United States in North Carolina, where palms are part of the native flora.

Eocene hyperthermal events drove episodes of vegetation turnover in the Fushun Basin, northeast China: Evidence from a palaeoclimate analysis of palynological assemblages

The response of terrestrial ecosystems to Eocene hyperthermal events remains poorly understood. In this paper, we report palynological assemblages from an astronomically-calibrated Eocene succession in the Fushun Basin of northeast China, in which previous δ13C studies have identified the Eocene Thermal Maxima 2 and 3 (ETM2, ETM3), the Early Eocene Climatic Optimum (EECO), and the Middle Eocene Climatic Optimum (MECO). In the samples that are directly associated with hyperthermal events, palynoassemblages show significant increases in the abundance of conifer pollen, especially Abietineaepollenites and Taxodiaceaepollenites, and fern spores of Polypodiaceaesporites, while angiosperm taxa reduce in frequency. Quantitative palaeoclimate parameters derived through the application of the Coexistence Approach to palynological assemblages indicate marked changes in temperature and humidity during hyperthermal events. These changes were most significant during ETM2 when mean annual temperature increased by 3–5 °C and mean annual precipitation increased by approximately 600 mm above background levels, but similar perturbations are also associated with the other hyperthermal events. An increase in organic abundance in lacustrine oil shale facies during hyperthermal events may imply that elevated humidity drove enhanced runoff, nutrient supply, and eutrophication of the hydrologically-open lake basins at a time when terrestrial carbon export from humid forests was also enhanced. Findings demonstrate that short-term bursts of Eocene warming drove major turnovers in the mid-latitude vegetation of Asia, reworking landscapes, and transforming regional hydrology.

NEW APPROACH TO SEPARATE AND DATE SMALL SPORES AND POLLEN FROM LAKE SEDIMENTS IN SEMI-ARID CLIMATES

ABSTRACTThe dating of pollen grains is emerging as the method of choice for lacustrine climate archives that contain few datable macrofossils. Due to the need for high-purity pollen concentrates, new methods are constantly being developed to precisely separate pollen grains. Flow cytometry represents a promising alternative to conventional approaches, enabling the identification of pollen grains through fluorescence and rapid separation for radiocarbon analysis using accelerator mass spectrometry, which has so far been limited to sediments with a high proportion of conifer pollen. We present a revised method for processing large sediment samples, resulting in high-purity pollen and spore concentrates. Using this approach small- to medium-sized pollen and bryophyte spores were isolated from Lake Van sediment samples (Eastern Anatolia, Turkey) in sufficient purity for radiocarbon dating. However, a systematic age discrepancy between pollen and bryophyte spore concentrates was noted. By adapting the chemical and cytometric methods, pure pollen concentrates can be created for sediments with low organic content enabling age determination of climate archives with a low proportion of large pollen or low pollen concentration.

Palaeoecology and palaeoclimate of an Early Cretaceous peat mire in East Laurasia (Hailar Basin, Inner Mongolia, China)

In this contribution, we report the palynology, organic petrography and carbon isotopes for the #16 Seam in the Lower Cretaceous Yimin Formation of the Hailar Basin, Inner Mongolia, China. Eighteen palynological samples were collected from the coal seam as well as from intraseam partings and the immediate roof. Microfloras comprise a diverse assemblage of ferns, conifers, horsetails, lycopsids, bryophytes, ginkgoes and cycads. Conifer pollen is common, especially representative of Pinaceae (Pinuspollenites) as well as Cheirolepidiaceae (Classopollis) and Araucariaceae (Araucariacites, Chasmatosporites); however, this material is interpreted as mostly derived from an upland source rather than from the mire-forming vegetation. An overrepresentation of conifer pollen may explain the relatively poor abundance and diversity of ginkgo and cycad pollen, compared to what is found in the macrofossil record. The palynological assemblage found in Seam #16 indicates a Barremian to Aptian age. Maceral composition, ash yields, and δ13Corg signature of the coal suggest peat accumulation was controlled by a rising water table from flooding of the mire, coupled with significant ground (peat) fires and/or surface wildfires. These conditions resulted in high levels of detrital mineral matter and charcoal input, the latter represented by inertodetrinite in the coals. The top of the coal seam is characterised by a higher ash yield, lower δ13Corg values and increased sphagnacean spores, which suggest the gradual drowning of the palaeomire. Although angiosperms were present in the Hailar Basin during the time that Seam #16 was accumulating, they are absent from the pollen found in this study. This may indicate that they were not yet adapted to the extreme conditions of the mire (e.g., acidity, anoxia), and unable to compete with the established mire vegetation.

Palynostratigraphic, palaeoenvironmental and palaeogeographic significance of the Early Cretaceous palynoflora of Kachchh Basin, western India

Sediments of Bhuj Formation (Early Cretaceous) occur in the Kachchh Basin, western India containing abundant and diverse plant fossils. Sedimentary successions of the formation exposed along Pur River comprise variably thickened shale, carbonaceous shale, thin coal seam, siliceous clays, etc. and have yielded abundant megaspores, microspores, conifer pollen grains, dinoflagellate cysts and colonies of Botryococcus alga. Two palynozones are recognized in the recorded palynoassemblage, viz. Minerisporites cutchensis megaspore zone at the basal part, derived mostly from herbaceous vegetation growing along pro–deltaic swampy habitat. The succeeding Araucariacites australis zone in the upper part of the succession probably derived from conifer–dominated forest inhabited along the freshwater swamps, flourishing in a more humid and warm temperate climate. The palynofloras of both palynozones are biostratigraphically very significant indicating Late Aptian–Early Albian age of the succession. A majority of the palynotaxa recorded from the studied sedimentary succession show resemblance with the contemporaneous deposits of other Gondwana continents of the Southern Hemisphere.

Paleoenvironment of the Cerro Negro Formation (Aptian, Early Cretaceous) of Snow Island, Antarctic Peninsula.

A study of macro and microfacies, palynoflora and palynofacies of the non-marine Cerro Negro Formation at President Head Peninsula, Snow Island, northwest of the Antarctic Peninsula, was developed. Two assemblages were recognized: Palynofacies assemblage 1 (P1) at the base of the section with a dominance of fern spores and conifer pollen grains, and facies association consisting of a clastic layer, with the predominance of mudstones; and Palynofacies assemblage 2 (P2) at the top of the section, with remarkable abundance of AOM/Pseudoamorphous particles, associated with facies that includes tuffs. The complete section shows in some levels the presence of freshwater algae and translucent phytoclasts. The integrated data characterizes a fluvial-lacustrine environment, what is reinforced by the occurrence of freshwater algae (Botryococcus) in some levels of P1 and P2. We could verify an increase in volcanic activity towards the top of the section that apparently has played an important role in the collapse of the palynoflora. The occurrence of the spore species Muricingulisporis annulatus, Sotasporites elegans, S. triangularis, Foraminisporis wonthaggiensis, and F. asymmetricus in the Cerro Negro Formation allows the correlation with sections in South America and Australia, suggesting an Aptian age for these deposits.

Субфоссильные спорово-пыльцевые спектры как отражение высотной поясности Южного Сихотэ-Алиня

The correspondence of pollen spectra from surface soil samples from the Olkhovaya Mountain (height 1669 m) to vertical belt vegetation (coniferous-broadleaved forests, spruce-fir forests and golets belt) was analyzed. Attention is paid to the analysis of the pollen spectra formed under alpine vegetation, including spruce forests at an altitude of 1600 m and on a swampy area near the Alekseevskoye Lake, a unique alpine lake in the Southern Sikhote-Alin. Pollen of woody vegetation prevailed in subfossil spectra from all vegetation belts, the composition of pollen of herbaceous plants and spores was poor. 16 taxa of arboreal, 8 taxa of nonarboreal, 5 taxa of spores were identified. It was found that the ratio of the main taxa, in general, corresponds to the dominant plants of the vegetation cover. It is shown that the local vegetation is not fully reflected in the subfossil spectra in the alpine belt, especially there is little pollen from shrubs and grasses, and waterlogged habitats are poorly reflected. It was revealed which pollen from the leading plant families and genera of flora of the highlands is not reflected in the pollen spectra. It has been established how much allochthonous pollen and what taxa were carried by the wind from lower relief levels. Taxa with remote sources were found. In general, the pollen spectra from the Olkhovaya Mountain highlands reflect the widespread development of spruce forests near the peak. Presence of spruce forests and a small area of the alpine zone are the main reasons explaining why forest pollen spectra with a predominance of dark coniferous pollen were obtained above the forest boundary. The results obtained were compared with the data on subfossil spectra from soils and surface peat of the bogs of the Sergeevskoye and Shkotovskoye plateaus, as well as the Partizanskaya and the Kievka rivers basins. The data obtained are important for more correct paleogeographical reconstructions: biomization methods in the mountainous areas of the south Far East and the development of methodological techniques for assessing quantitative paleoclimatic parameters.

A Lower Cretaceous palynoflora from Carregueira (Lusitanian Basin, westernmost Iberia): taxonomic, stratigraphic and palaeoenvironmental implications

Here we describe a new Lower Cretaceous palynoflora from the Lusitanian Basin, located in the westernmost sector of the Iberian Peninsula. The spore-pollen assemblage was extracted from samples collected in the Carregueira clay pit complex, located near the village of Juncal, western Portugal, from sedimentary deposits belonging to the Figueira da Foz Formation. A rich and well-preserved palynoflora, typical of non-marine fluvial environments, was recovered. Fifty-eight species and morphological groups of palynomorphs in 43 genera were recognized. The palynoflora is dominated by fern spores and conifer pollen. Angiosperm pollen is scarce and mainly represented by Afropollis, Clavatipollenites, Stellatopollis and Senectotetradites. Comparisons with described marine sections in Portugal suggest that the fossil horizon is lower Albian, corresponding to a hiatus between Zone I and Zone II in the Potomac Group of the eastern USA. The same beds contain a mesofossil flora dominated by angiosperm seeds, fruits and flowers. A similar contrast in the relative abundance of angiosperm mesofossils and pollen is seen at other Portuguese localities but is not evident in the Potomac Group. The palynoflora and the sedimentological data suggest deposition in wet lowland environments in a moderately humid regional setting.

Design of peptides with high affinity binding to a monoclonal antibody as a basis for immunotherapy

About half of the US population is sensitized to one or more allergens, as found by a National Health and Nutrition Examination Survey (NHANES). The most common treatment for seasonal allergic responses is the daily use of oral antihistamines, which can control some of the symptoms, but are not effective for nasal congestion, and can be debilitating in many patients. Peptide immunotherapy is a promising new approach to treat allergic airway diseases. The small size of the immunogens cannot lead to an unwanted allergic reaction in sensitized patients, and the production of peptides with sufficient amounts for immunotherapy is time- and cost-effective. However, it is not known what peptides are the most effective for an immunotherapy of allergens. We previously produced a unique monoclonal antibody (mAb) E58, which can inhibit the binding of multiple groups of mAbs and human IgEs from patients affected by the major group 1 allergens of ragweed (Amb a 1) and conifer pollens (Jun a 1, Cup s 1, and Cry j 1). Here, we demonstrated that a combined approach, starting from two linear E58 epitopes of the tree pollen allergen Jun a 1 and the ragweed pollen allergen Amb a 1, and residue modifications suggested by molecular docking calculations and peptide design could identify a large number of high affinity binding peptides. We propose that this combined experimental and computational approach by structural analysis of linear IgE epitopes and peptide design, can lead to potential new candidates for peptide immunotherapy.

Late Jurassic-Early Cretaceous palynostratigraphy and palaeoclimate in the Andigama Basin, Sri Lanka

Sri Lanka consists of only ten percent of sedimentary rocks, and high-grade metamorphic rocks underlie the rest. Most of these sedimentary terrains are post-Gondwanic and such formations help to understand the geological history of the island. We report the first record of an age diagnostic (Late Jurassic-Early Cretaceous) palynological assemblage of the sedimentary rocks in the Andigama Basin (borehole AND BH01) Sri Lanka. The study was undertaken to assess the palaeoenvironmental settings in this basin based on palynological and palynofacies investigations. A palynofloral study suggests luxuriant gymnospermous forests proliferation in the Andigama Basin during this time. The palynological assemblage is characterized by the predominance of coniferous pollen grains of Araucariacites spp., Callialasporites spp. along with some stratigraphically significant taxa viz., Cicatricosisporites spp., Aequitriradites verrucosus, Triporoletes sp., Impardecispora indica, Concavissimisporites verrucatus, Distaltriangulisporites perplexus, Verrucosisporites verrucosus, and Contignisporites fornicates of Tithonian-Berriasian time. Palynofacies records suggest three distinct Palynofacies Assemblages (PF 1–3). PF 1 indicates the forest swamp; PF 2 represents the mixed assemblage of forest swamp and reed marshes, while PF 3 suggests reed marshes/lakeshore deposits under oxic-anoxic environments. These palynofacies assemblage along with variable lithological counterparts varying from carbonaceous shale laminae interlayered with calcareous sandstone to brown shale and carbonaceous shale strata reflects diverse hydrodynamic conditions. Palynofloral and palynofacies records of the present study denote the warm and humid climatic conditions, which directly corroborates with global oxygen isotopic studies of the Late Jurassic-Early Cretaceous sediments.