Water Availability Conditions Research Articles

Pinus halepensis somatic embryogenesis is affected by the physical and chemical conditions at the initial stages of the process

Pinus halepensis has been described as a drought-tolerant species with high plasticity to growth in different environments. Its eco-physiological characteristics could facilitate the use of this species in large afforestations in the future scenery of climate change. Somatic embryogenesis is a biotechnological tool with potential for large-scale clonal propagation. In order to establish an improved regeneration protocol for Pinus halepensis, the effects of different temperatures (18, 23, and 28 °C) and water availability conditions (2, 3, and 4 g L−1 Gelrite®), during initiation of embryonal masses on the rate of initiation, proliferation, maturation, and the number of embryos developed, were evaluated. It was found that environmental conditions during the initiation stage of Pinus halepensis somatic embryogenesis influence the success of initiation and proliferation. In contrast, there was no effect of these conditions on the maturation rates and the number of somatic embryos. Somatic embryos were obtained in all treatments tested, indicating that plants can be produced from extreme conditions of induction, such as high temperatures (28 °C) and low water availability conditions (4 g L−1).

Glyphosate Resistance in Perennial Ryegrass (Lolium perenneL.) is Associated with a Fitness Penalty

The net selection effect of herbicides on herbicide-resistance traits in weeds is conditioned by the fitness benefits and costs associated with resistance alleles. Fitness costs play an important evolutionary role preventing the fixation of adaptive alleles and contributing to the maintenance of genetic polymorphisms within populations. Glyphosate is widely used in world agriculture, which has led to the evolution of widespread glyphosate resistance in many weed species. The fitness of glyphosate-resistant and -susceptible perennial ryegrass plants selected from within a single population were studied in two field experiments conducted during 2011 and 2012 under different soil water availability. Glyphosate-resistant plants showed a reduction in height of 12 and 16%, leaf blade area of 16 and 33%, shoot biomass of 45 and 55%, seed number of 33 and 53%, and total seed mass of 16 and 5% compared to glyphosate-susceptible plants in 2011 and 2012, respectively. The reduction in seed number per plant resulted in a 40% fitness cost associated with the glyphosate-resistance trait in perennial ryegrass. Fitness costs of glyphosate-resistant plants were expressed under both conditions of water availability. These results could be useful for designing management strategies and exploiting the reduced glyphosate-resistant perennial ryegrass fitness in the absence of glyphosate selection.

A data fusion‐based drought index

AbstractDrought and water stress monitoring plays an important role in the management of water resources, especially during periods of extreme climate conditions. Here, a data fusion‐based drought index (DFDI) has been developed and analyzed for three different locations of varying land use and climate regimes in Australia. The proposed index comprehensively considers all types of drought through a selection of indices and proxies associated with each drought type. In deriving the proposed index, weekly data from three different data sources (OzFlux Network, Asia‐Pacific Water Monitor, and MODIS‐Terra satellite) were employed to first derive commonly used individual standardized drought indices (SDIs), which were then grouped using an advanced clustering method. Next, three different multivariate methods (principal component analysis, factor analysis, and independent component analysis) were utilized to aggregate the SDIs located within each group. For the two clusters in which the grouped SDIs best reflected the water availability and vegetation conditions, the variables were aggregated based on an averaging between the standardized first principal components of the different multivariate methods. Then, considering those two aggregated indices as well as the classifications of months (dry/wet months and active/non‐active months), the proposed DFDI was developed. Finally, the symbolic regression method was used to derive mathematical equations for the proposed DFDI. The results presented here show that the proposed index has revealed new aspects in water stress monitoring which previous indices were not able to, by simultaneously considering both hydrometeorological and ecological concepts to define the real water stress of the study areas.

Morpho-physiological response to drought of progenies of Pinus taeda L. contrasting in mean growth rate

Fil: Bulfe, Nardia Maria Lujan. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Misiones. Estacion Experimental Agropecuaria Montecarlo; Argentina

Drought tolerance of three olive cultivars alternatively selected for rain fed or intensive cultivation

Physiological drought response was evaluated for two olive cultivars commonly grown under rain fed conditions (‘Souri’ and ‘Picual’) and another selected for intensive, irrigated cultivation (‘Barnea’). ‘Souri’ is a traditional local Israeli cultivar, ‘Picual’ originated in Spain and ‘Barnea’ is a modern Israeli cultivar.Trees in pots were alternatively provided well irrigated conditions (100% FC, field capacity) or allowed to dry, first to 33% FC and then to 10% FC. Under conditions of greatest water availability, the ‘Barnea’ cultivar had the highest stomatal conductance and net photosynthesis, significantly higher than that found in ‘Souri’. Stomatal conductance and leaf water potential of ‘Souri’ and ‘Picual’ at 33% FC were not affected relative to the well irrigated treatment but decreased significantly at 10% FC. Photosynthetic parameters of ‘Souri' and ‘Picual’ were not affected by water stress. Stem growth was also not affected by drought in ‘Souri’ but was reduced at 10% FC in ‘Picual’. In contrast, the ‘Barnea’ showed higher sensitivity to low water availability with stomatal conductance and net photosynthesis reduced at 33% FC and sharp decreases in these and leaf water potential occurring at 10% FC. At 10% FC ‘Barnea’ trees showed stem shrinkage, a phenomenon not observed in the other cultivars. These results suggest a tradeoff between selection for suitability in intensively irrigated orchards and tolerance to drought.

Growth and photosynthetic CO2 responses of date palm plants to water availability

Date palm is known to survive and withstand harsh environment, especially under scarce water availability and extreme dry conditions. Drought adaptation in plant species includes evolutionary consequences detectable at a wide range of morphological, genetic as well as physiological multifunctional responses. Although, significant researches have been reported dealing with water relations of date palms to agronomic traits and yield performance, yet, revealing the mechanism of date palm adaptation to drought needs adequate attention. In this study, the aim was to examine the growth adjustments and photosynthetic CO2 (Carbon dioxide) responses of date palm plants to water availability. The experimental methodology included long-term irrigation treatments based on field capacity measures, evaluation of the biochemical gas exchange model and assessment of growth and photosynthetic performance. As results, a variety of morphological as well as physiological adjustments were observed to take place in response to water availability. Accelerated leaf senescence, reduced green area as well the balanced root: shoot ratio were among the main morphological adjustments observed. Stomatal conductance (gs) showed a slight decrease in response to increasing external supply of CO2 from ambient to 1,500 ppm (part per million), while intercellular CO2 (Ci) continued increasing up to the highest tested level. As consequences, the increasing CO2 significantly (P ≤ 0.001) increased the net photosynthetic rate of the intact leaves of date palm plants with significant (P ≤ 0.001) gas-water interaction effect. Reduction of water by 50% of field capacity did not generate significant effect on the photosynthetic ability of the tested plants; this result also confirmed by the slight changes in the biochemical reaction indices. However, at 25% and 10% water availability changes in responses become more pronounced. On the other hand, the reduction of gs by 30% at 10% water availability compared to 100% did not affect the increasing pattern of Ci/Ca ratio (intercellular CO2 concentration / gas external supply ratio) of elevated CO2 when compared to that of the ambient; an observation might indicate a non-absolute stomatal role among the determinants of photosynthetic capacity of date palms in response to water availability. The observed growth adjustments, the indexed changes in the photosynthetic biochemical reactions as well as the implications for date palm adaptation to drought are considered in the discussion.

Adaptive phenotypic plasticity and competitive ability deployed under a climate change scenario may promote the invasion of Poa annua in Antarctica

Antarctica is one of the less prone environments for plant invasions, nevertheless a growing number of non-native species have been registered in the last decades with negative effects on native flora. Here we assessed adaptive phenotypic plasticity in three photoprotective traits (non-photochemical quenching, total soluble sugars, and de-epoxidation state of xanthophylls cycle), and fitness-related traits (maximum quantum yield, photosynthetic rate and total biomass) in the invasive species Poa annua and Deschampsia antarctica under current conditions of water availability and those projected by climate change models. In addition, two manipulative experiments in controlled and field conditions were conducted to evaluate the competitive ability and survival of both species under current and climate change conditions. Moreover, we performed an experiment with different water availabilities to assess cell damage as a potential mechanism involved in the competitive ability deployed in both species. Finally, was assessed the plasticity and biomass of both species subject to factorial abiotic scenarios (water × temperature, and water × nutrients) ranging from current to climate change condition. Overall, results showed that P. annua had greater phenotypic plasticity in photoprotective strategies, higher performance, and greater competitive ability and survival than D. antarctica under current and climate change conditions. Also, cell damage, assessed by lipid peroxidation, was significantly greater in D. antarctica when grown in presence of P. annua compared when grown alone. Finally, P. annua showed a greater plasticity and biomass than D. antarctica under the factorial abiotic scenarios, being more evident under a climate change scenario (i.e., higher soil moisture). Our study suggests that the high adaptive plasticity and competitive ability deployed by P. annua under current and climate change conditions allows it to cope with harsh abiotic conditions and could help explain its successful invasion in the Antarctica.

Dynamics of production and accumulation of phytolith assemblages in the Restinga of Maricá, Rio De Janeiro, Brazil

Abstract The Marica “restinga”, located on the eastern part of Rio de Janeiro State (Brazil), corresponds to one of the few remaining preserved areas of the state's coastal plain. This coastline presents two sand barriers separated by a depression where there is a freshwater swamp and different plant communities distributed in areas of high ecological diversity accompanying the local topography and the range of marine spray. This paper is a study of the soil phytolith assemblages in the Marica restinga, with the intent of establishing reference collections for paleoenvironmental studies of this coastal area during the Quaternary. Phytoliths were chosen as proxies, but their use for reconstruction of this type of vegetation is pioneer research, so it was essential to characterize the phytolith assemblages for each plant community through the identification of the main morphotypes and whether or not they are preserved in coastal depositional environments under the effect of geomorphology and salinity. Eight plant communities, distributed almost parallel to the coastline over sandy barriers, lagoonal plain, lagoon margin and weathered basement were identified: Halophile-psamophile, near the beach; two Scrub communities, one over the Holocene barrier and the other over the Pleistocene barrier; two Herbaceous swamps, one between the two sand barriers, the other behind the Pleistocene barrier; Slack, in front of the Pleistocene barrier; Shrubby vegetation on the shore of Marica lagoon and Dry forest, over the weathered Pre-Cambrian basement. In general, it was found that this type of environment is not very favorable for the formation and accumulation of phytoliths. Restinga plants did not prove to be large producers of phytoliths, except those known as such, as in the case of Poaceae and Cyperaceae. It was also observed that the permanence of phytoliths in sediments is strongly influenced by its particle size and organic matter content. The largest stocks of phytoliths were found in swamp communities, due to the presence of organic matter and low energy dynamics, and in dry forests, where a process of pedogenesis already occurs. Despite the low phytolith stocks present in modern assemblages, they show a high degree of conservation, which enabled significant results for the study of this type of vegetation. The calculated phytolith indices (tree density and water stress) consistently expressed, in detail, the environment of each community analyzed from the point of view of the type of vegetation cover and the conditions of water availability to plants.

LEAF TRAITS AND PLASTIC POTENTIAL OF PLANT SPECIES IN A LIGHT-EDAPHIC GRADIENT FROM RESTINGA IN SOUTHERN BRAZIL

Restinga formations grow on sandy spits of coastal plains, an environment whose conditions limit the growth and development of vegetal species. Studies on restinga gradients are good examples of how plants acclimate to restrictive environments. This work compares three woody species co-occurring in four vegetations of a restinga from Southern Brazil. It pinpoints morpho-anatomical attributes that favor the survival of species faced with spatial variability of soil and light conditions. Results indicate that they respond differently to environmental variables on different scales. The plastic response of morphological attributes is more marked than that of anatomical ones. Varronia curassavica and Dodonaea viscosa showed more xeromorphic features on the more stressful restinga formations while Symphyopappus casarettoi varied between xerophyte to mesophyte forms along the gradient. Individual height, fresh and dry leaf masses, leaf area, specific leaf mass and area, leaf density, and water content are particularly noteworthy. These responses are strategies allowing the studied species to survive in restinga environment with highly variable soil nutrient, water availability, and light conditions. The environmental conditions are important features that modulate de plant morphology along the gradient. Keywords: coastal vegetation, leaf morphology, leaf anatomy, light and soil conditions.

Isolation of culturable mycobiota from agricultural soils and determination of tolerance to glyphosate of nontoxigenic Aspergillus section Flavi strains

ABSTRACTGlyphosate-based herbicides are extensively used in Argentina's agricultural system to control undesirable weeds. This study was conducted to evaluate the culturable mycobiota [colony forming units (CFU) g−1 and frequency of fungal genera or species] from an agricultural field exposed to pesticides. In addition, we evaluated the tolerance of A. oryzae and nontoxigenic A. flavus strains to high concentrations (100 to 500 mM – 17,000 to 84,500 ppm) of a glyphosate commercial formulation. The analysis of the mycobiota showed that the frequency of the main fungal genera varied according to the analyzed sampling period. Aspergillus spp. or Aspergillus section Flavi strains were isolated from 20 to 100% of the soil samples. Sterilia spp. were also observed throughout the sampling (50 to 100%). Aspergillus section Flavi tolerance assays showed that all of the tested strains were able to develop at the highest glyphosate concentration tested regardless of the water availability conditions. In general, significant reductions in growth rates were observed with increasing concentrations of the herbicide. However, a complete inhibition of fungal growth was not observed with the concentrations assayed. This study contributes to the knowledge of culturable mycobiota from agricultural soils exposed to pesticides and provides evidence on the effective growth ability of A. oryzae and nontoxigenic A. flavus strains exposed to high glyphosate concentrations in vitro.

Sensitivity of water scarcity events to ENSO-driven climate variability at the global scale

Abstract. Globally, freshwater shortage is one of the most dangerous risks for society. Changing hydro-climatic and socioeconomic conditions have aggravated water scarcity over the past decades. A wide range of studies show that water scarcity will intensify in the future, as a result of both increased consumptive water use and, in some regions, climate change. Although it is well-known that El Niño–Southern Oscillation (ENSO) affects patterns of precipitation and drought at global and regional scales, little attention has yet been paid to the impacts of climate variability on water scarcity conditions, despite its importance for adaptation planning. Therefore, we present the first global-scale sensitivity assessment of water scarcity to ENSO, the most dominant signal of climate variability. We show that over the time period 1961–2010, both water availability and water scarcity conditions are significantly correlated with ENSO-driven climate variability over a large proportion of the global land area (> 28.1 %); an area inhabited by more than 31.4 % of the global population. We also found, however, that climate variability alone is often not enough to trigger the actual incidence of water scarcity events. The sensitivity of a region to water scarcity events, expressed in terms of land area or population exposed, is determined by both hydro-climatic and socioeconomic conditions. Currently, the population actually impacted by water scarcity events consists of 39.6 % (CTA: consumption-to-availability ratio) and 41.1 % (WCI: water crowding index) of the global population, whilst only 11.4 % (CTA) and 15.9 % (WCI) of the global population is at the same time living in areas sensitive to ENSO-driven climate variability. These results are contrasted, however, by differences in growth rates found under changing socioeconomic conditions, which are relatively high in regions exposed to water scarcity events. Given the correlations found between ENSO and water availability and scarcity conditions, and the relative developments of water scarcity impacts under changing socioeconomic conditions, we suggest that there is potential for ENSO-based adaptation and risk reduction that could be facilitated by more research on this emerging topic.

Antioxidant system response in coffee seedlings under waterlogging.

We investigated the response from antioxidant system of Coffea arabica L. seedlings from Catuai and Mundo Novo cultivars to waterlogging. For this, acclimated seedlings with eight pairs of completely expanded leaves were submitted to three conditions of water availability: field capacity, intermittent waterlogging and continuous waterlogging. The evaluations were performed at the beginning of the experiment and five months after waterlogging. Activity of antioxidant enzymes and levels of ascorbate were evaluated. There was a response of enzymatic and non-enzymatic antioxidant system of coffee seedlings to stress condition, allowing the plants to survive under waterlogging for 150 days. The antioxidant system was more active in Catuai than in Mundo Novo cultivar.

The Effective Role of Mycorrhizal Symbiosis in Sinking CO<sub>2</sub> from Atmosphere of Mega Cities

An effort was made after detailed literature survey and few experiments, conducted at Laboratory conditions about the VAM fungus inoculated plants; they have large surface area and more photosynthetic rate, can assimilate more CO2, even can grow in drought condition including water deficiency and high temperature. For this purpose, a greenhouse pot experiment was conducted in which soil manifested with fungi was used and Conocarpus erectus L (common on green belt of Karachi Streets) was selected for testing the fungal engineering. Results demonstrated a well-developed strong roots system and branching pattern of shoots rather than larger surface area of leaves of the fungal engineered plant when compared with non-treated ones. The long root system indicates the stability of plant and water transport system in high temperature and low water conditions. While increased branching pattern of areal part may be directly related to an increase in net photosynthetic rates or increase CO2 absorption in the fungal inoculated plants. This investigation showed an interesting use of VAM services for technology development of root organ culture development in areas of low water availability and high temperature condition with elevated concentration of CO2. A mechanism of absorption of CO2 related with the alteration in plant bio-physical metabolism is discussed in relation with phosphorus uptake under VAM inoculation.

Living in biological soil crust communities of African deserts—Physiological traits of green algal Klebsormidium species (Streptophyta) to cope with desiccation, light and temperature gradients

Green algae of the genus Klebsormidium (Klebsormidiales, Streptophyta) are typical members of biological soil crusts (BSCs) worldwide. The phylogeny and ecophysiology of Klebsormidium has been intensively studied in recent years, and a new lineage called superclade G, which was isolated from BSCs in arid southern Africa and comprising undescribed species, was reported. Three different African strains, that have previously been isolated from hot-desert BSCs and molecular-taxonomically characterized, were comparatively investigated. In addition, Klebsormidium subtilissimum from a cold-desert habitat (Alaska, USA, superclade E) was included in the study as well. Photosynthetic performance was measured under different controlled abiotic conditions, including dehydration and rehydration, as well as under a light and temperature gradient.All Klebsormidium strains exhibited optimum photosynthetic oxygen production at low photon fluence rates, but with no indication of photoinhibition under high light conditions pointing to flexible acclimation mechanisms of the photosynthetic apparatus. Respiration under lower temperatures was generally much less effective than photosynthesis, while the opposite was true for higher temperatures. The Klebsormidium strains tested showed a decrease and inhibition of the effective quantum yield during desiccation, however with different kinetics. While the single celled and small filamentous strains exhibited relatively fast inhibition, the uniserate filament forming isolates desiccated slower. Except one, all other strains fully recovered effective quantum yield after rehydration. The presented data provide an explanation for the regular occurrence of Klebsormidium strains or species in hot and cold deserts, which are characterized by low water availability and other stressful conditions.

Estimating groundwater use patterns of perennial and seasonal crops in a Mediterranean irrigation scheme, using remote sensing

This work explores the use of satellite-based vegetation indices (VI) to study groundwater use in a semi-arid agricultural irrigated area. The objective is to obtain insight in spatial and temporal patterns and differences in groundwater usage of perennial (mainly fruit trees) and seasonal crops (mainly row vegetable crops) under varying climatic conditions. Cropping intensities of seasonal crops are derived for each sector and irrigation water applied (IWA) is calculated using VI-based (NDVI from MODIS) actual evapotranspiration estimates and local efficiency factors. Groundwater use is then derived as the residual of total IWA and surface water supplies for each sector and crop type. The results of IWA following this methodology were compared with survey-based results for two crop types. Results correlated well, but deviate most during drought period, likely due to salt leaching practices. Monthly groundwater use patterns and spatial and temporal differences during normal water availability and drought conditions are reported. On average, about 50% of irrigation water is extracted from aquifers, but during droughts this percentage increases considerably. Perennial crops show sharper increases in groundwater use under such conditions than seasonal crops. Overall, seasonal crops put more pressure on the groundwater resource than perennial crops. Our results and methodology will be useful for water resource managers, and policy makers concerned with the role of groundwater resources on the sustainability of semiarid agricultural regions.

Restrição fotossintética de plantas de soja sob variação de disponibilidade hídrica

O objetivo do trabalho foi caracterizar o metabolismo primário, com ênfase na atividade fotossintética, de plantas de soja sob diferente disponibilidade hídrica. O experimento foi conduzido em câmara de crescimento com plantas jovens de soja cv. BMX Apolo RR, cultivadas sem restrição hídrica até o estádio de seis folhas trifolioladas expandidas e a sétima folha aberta.Foram estudados cinco níveis de disponibilidade hídrica(-0,004, -0,006, -0,026, -0,042 e -0,164MPa) com oito vasos (repetições) por tratamento. Avaliou-se a assimilação líquida de carbono (AL), condutância estomática (gs) e transpiração (E) na sexta folha de uma planta de cada vaso. Foi determinado o potencial da água na folha, fluorescência da clorofila,conteúdo de clorofila (a, b e total), rendimento quântico e caracterizado o crescimento vegetativo. Potenciais da água no solo de -0,026 MPa comprometem a assimilação líquida de carbono de plantas de soja em função da redução da condutância estomática. Os potenciais aplicados não influenciam na quantidade de pigmentos fotossintéticos e no crescimento vegetativo em situações de breve exposição ao estresse.

Desempenho produtivo e retorno econômico da cultura do sorgo submetida à irrigação deficitária

RESUMO A irrigação deficitária é comumente utilizada em regiões de reduzida disponibilidade de água ou de energia para a irrigação e visa incrementar a eficiência de uso da água. Essa técnica pode ser adequada à cultura do sorgo, que apresenta produtividades satisfatórias, mesmo em condições limitadas de disponibilidade de água no solo. Os objetivos do trabalho foram: avaliar os efeitos de diferentes manejos de irrigação deficitária sobre o índice de área foliar, altura de plantas e rendimento de grãos do sorgo, e identificar o nível crítico de irrigação deficitária que proporciona maior produtividade da água irrigada e retorno econômico. O experimento foi desenvolvido no ano agrícola de 2012/2013, sob uma cobertura móvel, que impedia a ocorrência de chuva na área experimental. Foram testados quatro manejos de irrigação deficitária: 100; 75; 50 e 25% de reposição da evapotranspiração da cultura (ETc). A redução de 25% na reposição da ETc resultou na diminuição de 7% na altura das plantas, 5% no índice de área foliar e 17% no rendimento de grãos. A maior receita líquida da cultura foi alcançada utilizando o manejo de irrigação com reposição de 75% da ETc. A produtividade da água e o retorno econômico da aplicação de água apresentaram uma resposta linear positiva em relação à redução na reposição da ETc.

Negative effects of heterospecific pollen receipt vary with abiotic conditions: ecological and evolutionary implications

Studies that have evaluated the effects of heterospecific pollen (HP) receipt on plant reproductive success have generally overlooked the variability of the natural abiotic environment in which plants grow. Variability in abiotic conditions, such as light and water availability, has the potential to affect pollen-stigma interactions (i.e. conspecific pollen germination and performance), which will probably influence the effects of HP receipt. Thus, a more complete understanding of the extent, strength and consequences of plant-plant interactions via HP transfer requires better consideration of the range of abiotic conditions in which these interactions occur. This study addresses this issue by evaluating the effects of two HP donors (Tamonea curassavica and Angelonia angustifolia) on the reproductive success of Cuphea gaumeri, an endemic species of the Yucatan Peninsula. Mixed (conspecific pollen and HP) and pure (conspecific pollen only) hand-pollinations were conducted under varying conditions of water and light availability in a full factorial design. Reproductive success was measured as the number of pollen tubes that reached the bottom of the style. Only one of the two HP donors had a significant effect on C. gaumeri reproductive success, but this effect was dependent on water and light availability. Specifically, HP receipt caused a decrease in pollen tube growth, but only when the availability of water, light or both was low, and not when the availability of both resources was high. The results show that the outcome of interspecific post-pollination interactions via HP transfer can be context-dependent and vary with abiotic conditions, thus suggesting that abiotic effects in natural populations may be under-estimated. Such context-dependency could lead to spatial and temporal mosaics in the ecological and evolutionary consequences of post-pollination interactions.

Optimising Cropping Techniques for Nutrient and Environmental Management in Organic Agriculture

<p>Depth and architecture of root systems play a prominent role in crop productivity under conditions of low water and nutrient availability. The subsoil contains high amounts of nutrients that may potentially serve for nutrient uptake by crops including finite resources such as phosphorus that have to be used in moderation to delay their exhaustion. Biopores are tubular shaped continuous soil pores formed by plant roots and earthworms. Taproot systems especially those of perennial legumes can make soil nutrients plant available from the solid phase and increase the density of vertical biopores in the subsoil thus making subsoil layers more accessible for succeeding crops. Density of larger sized biopores is further enhanced by increased abundance and activity of anecic earthworms resulting from soil rest and amount of provided feed. Nutrient rich drilospheres can provide a favorable environment for roots and nutrient uptake of subsequent crops. Future efficient nutrient management and crop rotation design in organic agriculture should entail these strategies of soil fertility building and biopore services in subsoil layers site specifically. Elements of these concepts are suggested to be used also in mainstream agriculture headlands, e.g. as ‘Ecological Focus Areas’, in order to improve soil structure as well as to establish a web of biodiversity while avoiding constraints for agricultural production.</p>

Climatic origins predict variation in photoprotective leaf pigments in response to drought and low temperatures in live oaks (Quercus series Virentes)

Climate is a major selective force in nature. Exploring patterns of inter- and intraspecific genetic variation in functional traits may explain how species have evolved and may continue evolving under future climate change. Photoprotective pigments play an important role in short-term responses to climate stress in plants but knowledge of their long-term role in adaptive processes is lacking. In this study, our goal was to determine how photoprotective mechanisms, morphological traits and their plasticity have evolved in live oaks (Quercus series Virentes) in response to different climatic conditions. For this purpose, seedlings originating from 11 populations from four live oak species (Quercus virginiana, Q. geminata, Q. fusiformis and Q. oleoides) were grown under contrasting common environmental conditions of temperature (tropical vs temperate) and water availability (droughted vs well-watered). Xanthophyll cycle pigments, anthocyanin accumulation, chlorophyll fluorescence parameters and leaf anatomical traits were measured. Seedlings originating from more mesic source populations of Q. oleoides and Q. fusiformis increased the xanthophyll de-epoxidation state under water-limiting conditions and showed higher phenotypic plasticity for this trait, suggesting adaptation to local climate. Likewise, seedlings originating from warmer climates had higher anthocyanin concentration in leaves under cold winter conditions but not higher de-epoxidation state. Overall, our findings suggest that (i) climate has been a key factor in shaping species and population differences in stress tolerance for live oaks, (ii) anthocyanins are used under cold stress in species with limited freezing tolerance and (iii) xanthophyll cycle pigments are used when photoprotection under drought conditions is needed.