Practical Conditions Research Articles

Safety and Potential Efficacy of Expanded Umbilical Cord-Derived Mesenchymal Stromal Cells in Luminal Ulcerative Colitis Patients.

Inflammatory bowel disease (IBD) is characterized by periods of flare-ups and remission. It is likely to be an autoimmune in origin, presenting persistent therapeutic challenges despite current therapies. This study aims to investigate the potential of umbilical cord mesenchymal stromal cells (UCMSCs) in treating ulcerative colitis (UC). This study is a prospective phase 1 pilot, open-label, single-arm, and single-center study. UCMSCs were cultured under current Good Manufacturing Practice (cGMP) conditions and intravenously administered to six patients with UC. Safety and efficacy were evaluated using the Mayo Score/Disease Activity Index. Among the six enrolled adult patients, five completed long-term follow-ups. All exhibited at diagnosis active UC confirmed through comprehensive assessment methods. Each patient received three injections intravenously 2 weeks apart with a dose of 100 million UCMSC each. No significant short-term or intermediate-term adverse events were detected post-UCMSC administration. Long-term follow-up at 12 and 24 months showed sustained safety and no adverse events. Notably, three out of five patients achieved a Mayo score of 0 for UC, maintained at both 12 and 24 months, indicating a highly significant response (P < 0.001). This study demonstrates the safety and potential efficacy of UCMSCs in active UC. However, larger trials are warranted to validate these preliminary findings and to establish the role of UCMSC therapy as an option for managing UC.

Patients´ experiences of an exercise intervention in primary care following robot-assisted radical cystectomy due to bladder cancer: a qualitative study

BackgroundPhysical activity is thought to be a key component in reducing postoperative complications following major abdominal surgery. The available literature on exercise interventions following radical cystectomy in patients with bladder cancer is scarce but suggests that physical activity and exercise might improve physical function and health-related quality of life, thus calling for further investigation. The CanMoRe-trial is a single-blinded randomised controlled trial (Clinicals Trials NCT03998579 25/06/2019), aimed at evaluating the impact of an exercise intervention in primary care following robot-assisted radical cystectomy. This study seeks to explore patients’ experiences of the exercise intervention in the CanMoRe-trial to gain a better understanding of facilitating aspects and potential barriers.MethodsA qualitative study was conducted involving 20 patients from the intervention group of the CanMoRe-trial who were interviewed individually between October 2020 and March 2023 using a semi-structured interview guide. The interviews were recorded and transcribed verbatim and reflexive thematic analysis was used to analyse the data.ResultsFour main themes were identified: Having to adapt to new circumstances, describing the challenges regarding physical activity patients face after discharge. Optimising conditions for rehabilitation, describing how practical conditions affect patients’ ability to exercise. Motivated to get back to normal, describing patients´ desire to get back to normal life and factors influencing motivation. Importance of a supportive environment, describing the impact of social support, support from physiotherapists, and how the environment where exercise takes place impacts patients’ ability to exercise.ConclusionThis study found that patients participating in the CanMoRe-trial are positive towards physical exercise in PC following radical RARC. They are motivated to get back to normal life but face major challenges when arriving home following surgery, which affect their ability to perform physical activity and engage in exercise. Conditions need to be optimised to support patients’ ability to engage in exercise by providing an accessible PC location to perform exercise in. A supportive environment is also needed, including guidance from healthcare professionals regarding which type of exercise, intensity and amount of exercise that should be performed, enabling patients gradually to develop self-efficacy regarding exercise and focusing on goals related to patients’ normal lives before surgery.

Comorbid conditions in pediatric practice

The comorbidity is an urgent scientific and practical problem of modern pediatrics. The practical significance of combined pathology in children is determined by its widespread occurrence and universal impact on all aspects of a child’s health and treatment options. In patients with congenital heart defects, outcomes are influenced by numerous non-cardiac and genetic factors. In childhood and adolescent bronchial asthma, concomitant diseases are a common cause of adverse outcomes, such as poor disease control, frequent seizures, decreased quality of life and increased healthcare costs. A stable interdependence between the state of the gastrointestinal tract and the development of allergic or autoimmune diseases has been proven. The article describes a clinical case of late diagnosis of concomitant pathology in a child with chronic gastritis with clinical symptoms, diagnostic criteria and drug treatment options. The understanding the etiology and pathogenesis of concomitant conditions in children will help identify risk groups for the formation of chronic noncommunicable diseases and develop a plan for their prevention.

Multiscale simulation of liquid chromatography: Effective diffusion in macro–mesoporous beds and the B-term of the plate height equation

We performed multiscale simulations of analyte sorption and diffusion in hierarchical porosity models of monolithic silica columns for reversed-phase liquid chromatography to investigate how the mean mesopore size of the chromatographic bed and the analyte-specific interaction with the chromatographic interface influence the analyte diffusivity at various length scales. The reproduced experimental conditions comprised the retention of six analyte compounds of low to moderate solute polarity on a silica-based, endcapped, C18 stationary phase with water‒acetonitrile and water–methanol mobile phases whose elution strength was varied via the volumetric solvent ratio. Detailed information about the analyte-specific interfacial dynamics received from molecular dynamics simulations was incorporated through appropriate linker schemes into Brownian dynamics diffusion simulations in three hierarchical porosity models received from physical reconstructions of silica monoliths with a mean macropore size of 1.23 µm and mean mesopore sizes of 12.3, 21.3, or 25.7 nm. The mean mesopore size was found to have a similar influence on the effective mesopore diffusivity as the analyte polarity and the mobile-phase elution strength, which together determine the analyte residence time on a column. A smaller mesopore size attenuated the increase of the effective mesopore diffusivity with increasing mobile-phase elution strength significantly. The effective bed diffusivity was limited by the analyte residence time rather than by morphological details of the mesopore space. The stronger an analyte was retained by the chromatographic interface inside the mesopores, the slower became the mass transfer between the pore space hierarchies and the lower was the effective bed diffusivity. The B-terms of the plate height equation were finally generated with the bed diffusivities and phase-based retention factors derived from the hierarchical porosity models using additional information about the stationary-phase limit obtained from the analysis of analyte–bonded phase contacts. The B-terms highlight analyte- and mobile phase-specific behavior relevant to isocratic and gradient elution conditions in chromatographic practice. In particular, U-shaped B-term curves are observed due to the dominating contribution of the retention factor and the bed diffusivity to the B-term at low and high elution strength of the mobile phase, respectively.

The Predictors of Patient Safety Culture in Hospital Setting: A Systematic Review.

Patient safety (PS) is a global public health concern. It is estimated that 10% of patients experience preventable harm while hospitalized. Patient safety culture (PSC) has been recognized as essential to improving PS, drawing inspiration from other high-risk industries. In PS research, however, PSC poses conceptual challenges, with inconsistent terminology, a lack of definitions, and limited use of substantiating theory. Despite these challenges, PSC remains widely used in PS research and practice, as it is seen as a potential gateway to understanding sociotechnical complex aspects of the healthcare system and improving safe patient treatment and care. This review explores the concept of PSC in a hospital setting. How PSC is used as an outcome, thus exploring the theoretical position underpinning PSC, which predictors impact PSC, and how these predictors are related to PSC. Using a search of 3 electronic databases, 23 studies that met the inclusion criteria were selected for review. The review identified 81 predictors of PSC. Study population, unit of analysis and method varied widely. PSC as an outcome was assessed based on one of 4 surveys. Thus, the underpinning position of the PSC construct is dominated by an organizational/managerial approach. The large number of predictors explored and the range in outcome measures, units of analysis, and methods make it hard to establish any causal relationship. We argue that studies closer to actual practices in the messy conditions of clinical practice are needed.

Nonlinear finite element formulation for thin-walled conical shells

This study presents a novel finite element formulation to predict the geometrically nonlinear response of conical shells under a wide range of practical loading conditions. The formulation expresses the discretized equilibrium equations in terms of the first Piola-Kirchhoff stress tensor and its conjugate gradient of the virtual displacements, is based on the kinematics of Love-Kirchhoff thin shell theory and the Saint-Venant-Kirchhoff constitutive model, and captures the follower effect of pressure loading. The formulation takes advantage of the axisymmetric nature of the shell geometries by adopting a Fourier series to characterize the displacement distributions along the circumferential direction while using Hermitian interpolation along the meridional direction. Comparisons with general shell models show the accuracy of the formulation under various loading conditions with a minimal number of degrees of freedom, resulting in a significant computational efficiency compared to conventional general-purpose shell solutions.

Gold(III) Catalysis in Ionic Liquids: The Case Study of Coumarin Synthesis

AbstractWell‐defined (P,C)‐cyclometalated Au(III) complexes proved to be able to catalyze the synthesis of coumarins by intramolecular hydroarylation of a broad range of aryl propiolates under mild and practical conditions (0.1–2 mol% catalyst, 25–40 °C, 1–24 hours). The use of an ionic liquid as reaction solvent allowed to drastically decrease the amount of Brönsted acid used to unlock the catalyst regeneration step. The effect of the nature of the acid additive and of the ionic liquid anion have been assessed. Preliminary results on the extension of this methodology to the cyclization of aryl propargyl ethers are also presented.

ESTRATÉGIAS DE CONFORTO AMBIENTAL PARA QUADRAS FECHADAS VOLTADAS A ESPORTES DE ALTO RENDIMENTO

Sports architecture, with its particularities, is essential to optimize the efficiency of environments, considering the specific needs of each sport. This article addresses environmental comfort strategies applied to indoor courts intended for high-performance sports, highlighting the importance of an architecturally planned environment to ensure the well-being of athletes and optimize their performance. The study focuses on thermal, visual, acoustic and ergonomic aspects, and analyzes how each of these factors influences sports performance. In addition, it explores the interaction between the body and the physical environment, proposing solutions that favor the creation of functional and comfortable spaces for sports practice and competitive events. The research, of a bibliographic and exploratory nature, brings together concepts of environmental psychology and athletic performance, in addition to highlighting the construction strategies of ventilation, lighting and acoustic control that can be applied to indoor courts with the aim of promoting human well-being. The work proposes solutions so that sports centers offer adequate conditions for sports practice, supporting the creation of environments that favor both the satisfaction and the enhancement of the performance of athletes. From this study, it is possible to define the importance of the preparation and competition environment in the performance of high-performance athletes, and architecture, linked to environmental comfort, is shown to be a tool for the development of these spaces, essential for success in national and international sports competitions.

Typical tactics of treatment of patients with chronic non-specific back pain and tension-type headaches

Non-specific (musculoskeletal) back pain (NBP) and tension-type headache (THA) are among the most common conditions in neurological practice that significantly impair patients' ability to work. The typical practice of treating patients with NBP and THA has not been sufficiently investigated, which formed the basis for conducting this study.Objective: to evaluate the quality of diagnosis and treatment of chronic NBP and THA in real-life neurological practice.Material and methods. The study included 120 patients with chronic NBP and combined THA of different frequency (32 men and 88 women, mean age 38.4±9.6 years) who were treated in A.Ya. Kozhevnikov Clinic of Neurologic Diseases (CND) of Sechenov University with complaints of back pain and headache and who had previously been examined and treated by neurologists and therapists in other medical institutions on an outpatient and/or inpatient basis. All patients underwent a clinical interview to establish a diagnosis and analyze previous treatment, a neurological and neuro-orthopedic examination and a psychometric examination to assess anxiety and depression symptoms.Results. Prior to contacting CND, only 10% of patients had a diagnosis of chronic NBS, only 7% had a diagnosis of THA and the remaining patients had other misdiagnoses. All patients had previously undergone additional examinations, mainly neuroimaging of the spine and brain, ultrasound examination of the neck vessels, even if there were no "red flags" – signs of danger in back pain and headache. None of the patients had previously had their emotional state assessed, while 75% of patients had symptoms of depression of varying severity and all patients suffered from anxiety of varying severity. Neuro-orthopaedic examination was performed in only 10% of patients. All patients received nonsteroidal antiinflammatory drugs for back pain, and most patients received vascular and nootropic medication for headaches. Of the non-pharmacological methods, massage and passive physiotherapy were most frequently prescribed. Only 9% of patients were recommended kinesiotherapy, and only 5% of patients performed it.Conclusion. In real-life medical practice, chronic NBP and THA are inadequately diagnosed, excessive and unjustified additional examinations are prescribed, unsatisfactory level of treatment, and patients with chronic NBP and THA are rarely prescribed kinesiotherapy.

Clinical nurses’ legal roles, challenges, and responses to enabling legislation in China: a qualitative study

BackgroundWith the surge in China’s registered nurse population, defining their rights and responsibilities is crucial for ensuring the quality of medical services. However, challenges such as unresolved medical disputes and unclear duties persist due to the inadequacy and lack of transparency in current nursing laws and policies. This study evaluated nurses’ legal awareness, legislative needs, and views on laws and policies affecting their practice, providing insights for future improvements in nursing legislation.MethodsThis study employed a descriptive qualitative approach, utilizing purposive sampling to select 16 nurses for in-depth exploration of their experiences. Semi-structured interviews were conducted between December 2023 and March 2024. Data analysis was performed using directed content analysis.ResultsA total of 16 nurses with various positions and titles were interviewed. The analysis revealed three main themes and nine sub-themes, focusing on existing issues in the nursing environment, legal training needs, and attitudes towards legislative empowerment.ConclusionsIn China, there is a gap in legal knowledge and training among nurses. Enhancing their legal literacy is crucial for elevating their professional status and the quality of their work. Our findings underscore strong support for the enactment of the Nurse Act with a focus on practice conditions, rights protection, and career development. National authorities and experts must expedite this legislation to safeguard the rights of nurses, bolster their professional confidence.

Social workers’ support of the status quo: Results from a national survey

Summary Social work has an intimate and complex relationship with the systems that shape the social, political, economic, and environmental fabric of the United States. System justification theory helps explain why people are motivated to accept the status quo of systems and unequal social arrangements. This study examined the system-justifying beliefs of a national sample ( N = 516) of master’s-level social workers to better understand how beliefs about the status quo may shape job outcomes (e.g., burnout and satisfaction) and practice outcomes (e.g., goals, motivations, perceptions of clients, and endorsement of practice behaviors). Findings Ordinary least squares regression analyses adjusting for demographic characteristics indicated significant relationships with both practice outcomes and job outcomes. Higher system-justifying beliefs were associated with less social justice motivation, more practical motivation, less transformative practice goals, and less understanding of clients’ needs as symptoms of structural failures. However, higher system-justifying beliefs were also associated with more compassion satisfaction, less secondary trauma, and less emotional exhaustion. Applications Key interventions into the structural conditions of social work practice so that social workers rely less on system-justifying beliefs to cope with burnout and increase satisfaction are discussed. Examples include investments in support resources, workplace empowerment, increased discretion, and workload reduction. Cognitive and psychological interventions, specifically the development of critical consciousness and critical thinking, are also suggested.

Boosting self-powered wearable thermoelectric generator with solar absorber and radiative cooler

The electrical output of wearable thermoelectric generators (wTEGs) has traditionally been constrained by small temperature differentials when powering microelectronics. In this study, we innovatively combine photothermal and radiative cooling mechanisms within a single wTEGs system, enabling substantial, uninterrupted power generation. Specifically, we designed a multilayer selective solar absorber (m-SSA) composed of flexible dielectric-metal stacks. This absorber demonstrates exceptional solar absorption efficiency of 93 % and significantly low thermal emissivity of 10 %. In practical outdoor conditions, it achieves a temperature increase of up to 108 °C under solar irradiation. Concurrently, we developed a flexible hierarchically porous radiative cooler (HP-RC), which reflects 96 % of solar energy and emits 97 % of thermal energy, achieving a cooling differential of up to 10 °C, even at ambient temperatures of 42 °C. Integration of the m-SSA and HP-RC with wTEGs allows for the simultaneous harvesting of heat from solar, cold space, and earth (robots or human body). This novel energy capture mechanism yielded a notable power density of 198 mW/m² for human body and 52 mW/m2 for steel robots in outdoor wearable applications. This significant advancement promotes the field toward high-performance, integrated green power technologies and holds promise for next-generation wearable self-powered devices.

Inherent Water Competition Effect-Enabled Colloidal Electrode for Ultra-stable Aqueous Zn-I Batteries.

Electrode material stability is crucial for the development of next-generation ultralong-lifetime batteries. However, current solid- and liquid-state electrode materials face challenges such as rigid atomic structure collapse and uncontrolled species migration, respectively, which contradict the theoretical requirements for ultralong operation lifetimes. Herein, we present a design concept for a soft colloid polyvinylpyrrolidone iodine (PVP-I) electrode, leveraging the inherent water molecule competition effect between (SO4)2- from the electrolyte and PVP-I from the cathode in an aqueous Zn||PVP-I battery. Electrochemical demonstrations measured under various simulated and practical (integrated with photovoltaic solar panel) conditions highlight the potential for an ultralong battery lifetime. The PVP-I colloid exhibits a dynamic response to the electric field during battery operation. More importantly, the water competition effect between (SO4)2- from the electrolyte and water-soluble polymer cathode materials establishes a new electrolyte/cathode interfacial design platform for advancing ultralong-lifetime aqueous batteries.

On the influence of artificially distorted images in firearm detection performance using deep learning

Detecting people carrying firearms in outdoor or indoor scenes usually identifies (or avoids) potentially dangerous situations. Nevertheless, the automatic detection of these weapons can be greatly affected by the scene conditions. Commonly, in real scenes these firearms can be seen from different perspectives. They also may have different real and apparent sizes. Moreover, the images containing these targets are usually cluttered, and firearms can appear as partially occluded. It is also common that the images can be affected by several types of distortions such as impulse noise, image darkening or blurring. All these perceived variabilities could significantly degrade the accuracy of firearm detection. Current deep detection networks offer good classification accuracy, with high efficiency and under constrained computational resources. However, the influence of practical conditions in which the objects are to be detected has not sufficiently been analyzed. Our article describes an experimental study on how a set of selected image distortions quantitatively degrade the detection performance on test images when the detection networks have only been trained with images that do not present the alterations. The analyzed test image distortions include impulse noise, blurring (or defocus), image darkening, image shrinking and occlusions. In order to quantify the impact of each individual distortion on the firearm detection problem, we have used a standard YOLOv5 network. Our experimental results have shown that the increased addition of impulse salt-and-pepper noise is by far the distortion that affects the most the performance of the detection network.

Effective capture of As(V) from water by a facile one step hydrothermal synthesized of 2-D bismuthene quantum dots nanosorbent

Arsenic species have been known for their toxic impact on human. Therefore, removal of such pollutant requires efficient and effective removal methodology from polluted water. In this study, bismuthene quantum dots (Bi-ene-QDs) were fabricated by a green and facile one pot-hydrothermal conversion reaction of Bi(NO3)3·5H2O. Bi-ene-QDs exhibited semi-spherical crystalline providing 6.0 nm 157.78 m2/g. Consequently, As(V) capturing by Bi-ene-QDs revealed optimum practical conditions at pH 3, interaction duration time 40 min and 10 mg Bi-ene-QDs dosage. The interaction of As(V) ions with Bi-ene-QDs were confirmed by the appearance of As-O stretching vibration. Moreover, Bi-ene-QDs achieved excellent adsorptive capture percentages of Arsenic ions from sea, tap and wastewater providing 94.61, 95.21 and 94.38% from contaminated samples with 5 mg L−1 Arsenic ions. Therefore, Bi-ene-QDs can be categorized as an unprecedented and efficient nanosorbent for the successful removal of Arsenic ions pollution from various wastewater matrices with > 90.0% efficiency.

Bi-Functional Materials for Sulfur Cathode and Lithium Metal Anode of Lithium-Sulfur Batteries: Status and Challenges.

Over the past decade, the most fundamental challenges faced by the development of lithium-sulfur batteries (LSBs) and their effective solutions have been extensively studied. To further transfer LSBs from the research phase into the industrial phase, strategies to improve the performance of LSBs under practical conditions are comprehensively investigated. These strategies can simultaneously optimize the sulfur cathode and Li-metal anode to account for their interactions under practical conditions, without involving complex preparation or costly processes. Therefore, "two-in-one" strategies, which meet the above requirements because they can simultaneously improve the performance of both electrodes, are widely investigated. However, their development faces several challenges, such as confused design ideas for bi-functional sites and simplex evaluation methods (i. e. evaluating strategies based on their bi-functionality only). To date, as few reviews have focused on these challenges, the modification direction of these strategies is indistinct, hindering further developments in the field. In this review, the advances achieved in "two-in-one" strategies and categorizing them based on their design ideas are summarized. These strategies are then comprehensively evaluated in terms of bi-functionality, large-scale preparation, impact on energy density, and economy. Finally, the challenges still faced by these strategies and some research prospects are discussed.

A Scoping Review on the Utility of Ultrasound to Visualize Bursae in Anatomical Dissection Courses

Bursitis is a common condition in clinical practice, often causing pain in the shoulder and buttock areas due to inflamed bursae. Proper diagnosis and treatment depend on knowing the presence and exact location of these bursae. Anatomy classes typically provide limited instruction on bursae because they are difficult to demonstrate during dissection courses. High-resolution ultrasound is an essential and versatile technique for detecting bursitis, and it could also serve as a valuable tool for students to better understand bursae. Relevant studies were screened in the following databases: CENTRAL, MEDLINE, BIOSIS Previews, EMBASE, and Web of Science Core Collection. Grey literature was also considered. Literature was screened on January 3, 2023. Only ultrasound investigations in human cadaver bursae were included, specifically using B-Mode ultrasound. The general characteristics of the included studies and the ultrasound-guided approaches for labeling the bursae were analyzed and examined. T The search found 8,899 matches, but only 15 met the criteria. Fifteen different bursae were studied, and 12 studies were included in the analysis. Both the marking substrate and the injected volume varied. Despite a high overall accuracy of 99% achieved using ultrasound-guided labeling approaches in the included studies, caution is advised due to the small sample size (1 to 24). The current study serves as a review to examine ultrasound studies on bursae in human cadavers. Ultrasound-guided labeling techniques achieve high accuracy and could be a valuable teaching tool in dissection courses. These techniques help visualize difficult-to-dissect structures and provide students with an understanding of sonoanatomy.

Regeneration of sulfur-poisoned CeO2 catalysts via in situ treatment from NH3-SCR atmosphere

Ammonia selective catalytic reduction (NH3-SCR) is well utilized for NOx elimination, but the deactivation of SCR catalysts under practical conditions is a headache. Conventional methods for catalyst regeneration include heat treatment and solution leaching, which bear typical drawbacks of high energy cost, long time duration and requirement of off-site regeneration. In the present study, a novel regeneration strategy was proposed to alleviate SO2 poisoned SCR catalyst via in situ treatment under reaction atmosphere. The effects from operation atmosphere, temperature and duration of treatment time on regeneration efficiency were comprehensively investigated. It was confirmed that the ammonia contained in the reaction atmosphere greatly promoted sulfur removal from catalyst surface. Simultaneously, the crucial active sites with good redox property were replenished, which played an essential role in securing activity recovery. The result of present study is expected to provide useful guidance for regeneration of SCR catalysts poisoned by SO2.

Implementation of an app-based time-temperature-indicator system for the real-time shelf life prediction in a pork sausage supply chain

Time-temperature-indicators (TTIs) combined with predictive modeling are helpful tools for avoiding the increasing amount of food waste and the associated waste of resources along the food supply chain. Successful implementation of these systems in practice is still absent due to missing digital technologies for real-time shelf life prediction. This study aimed to validate a novel app system developed for the digital read-out of OnVu™ TTIs and the shelf life prediction of perishable products along the raw pork sausage supply chain. Therefore, a kinetic shelf life model of raw pork sausage was developed based on microbial parameters. A dynamic TTI model was developed based on app-measured TTI data and validated on a laboratory scale to prepare for the pilot study. In the pilot study, the shelf life prediction of TTIs based on app measurements was validated under practical conditions. Results showed that the spoilage kinetics of raw pork sausage could, in general, be reflected by the OnVu™ TTI kinetics based on the app's color measurements. The pilot study showed that predicted and measured TTI color values by the app were in good agreement with accuracy factors of 1.02–1.03; however, slight differences in shelf lives revealed that the prediction model must be further improved by integrating more data. Although variances in the hourly range could be seen between the predicted shelf life based on TTI app measurements and the real shelf life of raw pork sausage, the study serves as a proof of concept for the general useability of the app for shelf life prediction because it showed that TTI and product kinetics were highly comparable. Further technical adjustments to the app and the adaptation of the charging time may further improve the shelf life prediction by the app along the raw pork sausage supply chain.

Affordable and Reliable RP-HPLC Method for Verapamil Hydrochloride Quantification in Rabbit Plasma for Pharmacokinetics

Background: Existing bioanalytical methods for verapamil hydrochloride (VH) are often complex, requiring advanced instrumentation and specialized expertise, which limits their use in resource-constrained laboratories. Aim: The goal of this study is to fill this gap by developing a simplified, robust RP-HPLC-UV approach for the estimation of verapamil hydrochloride in rabbit plasma. Designed to enhance accuracy and precision while minimizing sample preparation challenges, this method addresses existing limitations by providing an affordable and reliable alternative for laboratories lacking sophisticated instrumentation. Methods: The bioanalytical method was implemented on C-18 stationary phase (5 μ, 250 × 4.6 mm) using acetonitrile/0.1% tetrahydrofuran (THF) in water (80:20, in volume) as the liquid system at a 1 mL/min flow speed, employing carvedilol as an internal standard. Results: The reported retention times of verapamil hydrochloride and carvedilol were ~7.64 and 4.69 min, respectively, at sufficiently high system suitability standards. The linearity of the bioanalytical approach can be seen between 0.025 and 5.0 µg/mL (r2 = 0.9991). The findings indicated that there was no matrix influence in terms of accuracy (≥98.96 ± 2.68%), intra- and inter-day precision (≤3.68%), recovery (101.98 ± 2.76%), and procedure efficiency (100.65 ± 1.82%). Benchtop, long-term, and short-term stability investigations all revealed that the verapamil hydrochloride in the bio-samples was stable. The pharmacokinetic parameters (Cmax—3.47 µg/mL; Tmax—1.59 h) were studied from time-dependent plasma concentrations of verapamil hydrochloride estimated after 40 mg oral dosing in New Zealand white rabbits. Conclusions: The developed bioanalytical method provided easier quantitative analysis of verapamil hydrochloride from rabbit plasma and was effectively used in a pharmacokinetic investigation of an oral bolus. The reliable performance of this method under practical conditions positions it as a crucial tool for advancing pharmacokinetic studies across various research environments.