Fish Condition Research Articles

Target miRNA identification for the LPL gene in large yellow croaker (Larimichthys crocea)

MicroRNA (miRNA), a conservatively evolved single-stranded non-coding RNA, exerts pivotal control over the appearance of target genes and several biological processes. This study conducted a comprehensive screening of candidate microRNAs (miRNAs) associated with Lipoprotein Lipase (LPL) in the large yellow croaker (Larimichthys crocea), utilizing sophisticated bioinformatics techniques across the species’ muscular and hepatic tissues. The bioinformatics analysis facilitated the compilation and examination of miRNA datasets specific to these tissues. The investigation culminated in the identification of miR-84a and miR-1231-5p as key miRNAs that modulate fat hydrolysis, highlighting their potential roles in lipid metabolism. Subsequent in-depth analysis further implicated these miRNAs, along with miR-891a, as prospective targets of LPL, suggesting their integral involvement in the regulation of this critical enzyme. Validation of these bioinformatics predictions was conducted through the construction of double luciferase reporters concealing the LPL 3′ untranslated region (3′UTR), substantiating that miR-84a and miR-1231-5p can modulate LPL expression via the LPL 3′UTR. Conversely, miR-891a was not concerned with this regulatory mechanism. Site-directed mutagenesis experiments elucidated the specificity of the interaction sequences. Quantitative PCR assays suggested that miR-84a and miR-1231-5p might influence LPL expression during the starvation phase, intimating the regulatory role of miRNA in fatty acid metabolism within hepatic and muscular tissue under starvation. These findings offer a nuanced understanding of LPL’s molecular functionality under stress conditions in fish, emphasizing the regulatory dynamics of miRNA during metabolic stress.

Analysis of Morphological Characteristics and Growth Patterns of Uceng Fish Nemacheilus fasciatus (Valenciennes, 1842) on the Setail River, Banyuwangi, East Java

Indonesia's. Setail River is one of the longest rivers in Banyuwangi Regency. Setail River has high potential for fishery biodiversity. One of the fish that is often found in Setail River is Barred loach or known locally as Uceng Fish (Nemacheilus fasciatus). The purpose of this study was to determine the morphological characteristics and determine the relationship between length, weight and condition of Uceng fish in Setail River. Observation of color patterns and special characteristics of sample fish morphologically Nemacheilus fasciatus with characteristics including Having a brown body color, a small elongated body (fusiform). On the body there is a longitudinal black spot pattern, a dorsal pattern resembling a saddle, a homocercal tail fin type, an emarginate tail morphology (slightly concave). The type of mouth is subterminal inferior and has 3 pairs of barbels. Meristic observations of uceng fish have pectoral fins, pelvic fins, dorsal fins, tail fins and anal fins. In this study, an analysis of the relationship between length and weight showed that the growth pattern of the uceng fish was negative allometric and the condition factor obtained the range of condition factor values for male uceng fish during the study at each station for male uceng fish 0.68-1.35 and female uceng fish 0.68-1.40.

How do extreme fluctuations in water level affect fish condition in Amazonian Floodplain Lakes?

The annual flood pulse is a defining feature of Amazonian floodplain lakes, creating a highly variable environment that influences resource availability, such as food and habitat. These cyclical changes necessitate a high degree of adaptability among fish species, many of which have evolved specialized strategies to cope with the fluctuating conditions. In 2023, the Amazon basin experienced a record-breaking drought event, leading to mass mortality of Amazonian fish and other wildlife. This study examines the effect of this extreme event on fish condition in white-water (Rio Solimões basin) and black-water (Rio Negro basin) floodplain lakes. These contrasting environments provide a unique opportunity to study how different water qualities and extreme water-level fluctuations impact fish condition. Research was conducted during the normal low-water period in November 2019 and the drastically decreased water levels in November 2023. The main objective was to understand how extreme water-level fluctuations affect fish health and nutritional status. A total of 585 fishes were analyzed, with 294 from white-water and 291 from black-water, representing different feeding types to provide a comprehensive picture of changes in fish condition. Water-level changes had a statistically significant impact on fish condition in both areas. Comparing low-water and extreme low-water levels, fish condition was consistently higher during the normal low-water period. The linear mixed-effects model revealed that the intensity of the low-water season had a significant effect on fish length-adjusted mass, suggesting that the decrease in water level is associated with an overall decrease in fish length-adjusted mass. When comparing the mean water-level effect (Glass's Δ) between low-water and extreme low-water levels, we found a bigger effect in the black-water system than in the white-water system. This difference may be attributed to the lower nutrient content and higher levels of humic acids and refractory dissolved organic matter in black-water, which can further limit primary productivity and food availability for fishes.

The effect of the genotype of holstein bulls on the phosphorus content in the liver of descendants

Russian and foreign scientists continue to systematize knowledge about the accumulation of macroand microelements in the body of animals, as their study remains an urgent topic for many studies. Questions about the hereditary determination of the level of macroand microelements in animal organs and tissues are also being studied. Currently, articles by Russian and foreign authors contain separate, disparate information about the macroand microelement status of farm animals and fish of different species. Our article describes data on the phosphorus content in the liver of offspring obtained from producing bulls of the Holstein breed. The research was conducted in Kuzbass. The studied animals were in the same conditions of maintenance and feeding. The groups were formed according to the principle of analogues. The same feeding and maintenance conditions were created for the experimental animals. In the animal breeding area, the content of macroand microelements in soil, water and feed was determined, the level of heavy metals was within acceptable concentrations. The phosphorus content in the liver was determined by atomic emission spectrometry on the ICP AES IRIS device. The phosphorus level in the offspring of the sons of different Holstein bulls was in the range of 2964–3417 mg/kg. In the liver of the descendants of some bulls, the phosphorus content was 1.15 times higher than in others. The index of the strength of the influence (RW) of fathers on the phosphorus content in the liver of sons was 0,49 (p < 0.05). This indicates the influence of the genotype of fathers on the phosphorus content in the liver of descendants. The level of phenotypic variability of phosphorus concentration in the liver of offspring of different bulls was in the range of 5.6–11.6 %. The obtained average values of phosphorus levels can be taken as the physiological norm for animals of the Holstein breed in Western Siberia. The study of the level of phosphorus and other elements in organs and tissues expands the understanding of the chemical status of animals and is a characteristic of the interior.

Effect of Food Deprivation on Plasma Cortisol, Carbohydrate Metabolism, and Histomorphology in Clarias batrachus.

The nutritional status of fish is essential for its health, experimental studies, and aquaculture practices. The current study investigated the impact of food deprivation on biochemical parameters, histology of skin, gill, and kidney tissues, and ultrastructure of gills in Clarias batrachus. Fish were subjected to food deprivation for 2, 7, and 15 days resulting in (a) significant increase in plasma cortisol levels, (b) no significant changes in plasma osmolality and plasma glucose content, and (c) significant decrease in liver and muscle glycogen contents. A substantial damage was detected in skin, gill, and kidney tissues with histological alterations in a time-dependent manner. Skin tissue displayed melanomacrophage aggregation, excoriated epidermis and dermis. In gill tissue, epithelial lifting, edema, desquamation, deformed secondary lamellae, and lamellar hyperplasia were observed. Kidney tissue exhibited degenerated tubules, melanomacrophage aggregations, and shrunken renal tubule. Scanning electron microscopy revealed that food deprivation-induced marked presence of mucus, chloride cells, and pavement cells with well-defined microridges and microbridges following 2 days, opening of chloride cells was more prominent after 7 days, while more mucus secretion was observed after 15 days. After food deprivation, alterations in biochemical and histological parameters, and ultrastructural changes in target tissues reflect physiological and morphological disturbances in fish. The novelty of this study is that these parameters can be considered as biomarkers of feeding stress in fish and fish health and can provide important insights for better aquaculture practices.

Diversity and Conservation Status of Gelodok Fish (Perciformes: Gobiidae) in East Pohuwato Coastal Area, Gorontalo Province, Indonesia

This study aims to determine the diversity and conservation status of Gelodok fish in the Pohuwato Coastal Mangrove Area. Data collection techniques were carried out by surveying the location to see the conditions and determine the sampling site. Sampling was done by cruising method using 3x3 meter size net. The fish found then analyzed the level of diversity and conservation status. The results showed that the spadefish found in the mangrove area of East Pohuwato Village consisted of two species of spadefish, namely Periophthalmus malaccensis and Periophthalmus argentilineatus with a diversity value of 0.17016 classified as low diversity. The conservation status of Periophthalmus malaccensis is included in the Data Deficient (DD) category and Periophthalmus argentilineatus is included in the Least Concern (LC) category based on the IUCN Red List of Threatened Species (IUCN)

Ulcerative dermal necrosis of mature Atlantic salmon in the Kola and Tuloma rivers (Murmansk region): retrospective and prospects for studying the problem

The aim of the work is to analyze the results of virological and immunological studies, to track the dynamics of the entry and changes in external signs of pathology of Atlantic salmon into the Kola and Tuloma rivers.Methods used: fish size and weight indicators were determined using generally accepted methods. Methods for isolating viruses on continuous cell lines and their identification in RT-PCR reaction were used. Bactericidal activity of blood serum (BABS) was assessed using a nephelometric method. The content of non-specific immune complexes (IC) was established using a spectrophotometric method with selective precipitation with PEG-6000.According to the results of the work, it was revealed that in the Kola and Tuloma rivers, clinical manifestations of pathologies in fish have changed. Since 2021, typical symptoms of UDN have been replaced by signs of «red skin disease». The results of immunological studies indicate high activity of innate humoral immunity mechanisms in the examined individuals. The carriage of infectious pancreatic necrosis virus in Atlantic salmon populations living in the Kola and Tuloma rivers has been established. Clinical signs of pathology in Atlantic salmon are described in detail, and fish counting data for the Kola and Tuloma rivers are presented.The novelty of the work: for the first time, photographs of all noted typical signs of pathology, as well as the results of virological and immunological studies of Atlantic salmon in the Kola and Tuloma rivers for 2022–2024 are presented. A retrospective overview is given and prospects for studying the problem are outlined.Practical significance: the conducted research will allow creating a database on the results of comprehensive studies of Atlantic salmon diseases in the Kola and Tuloma rivers. Compare the results obtained in fish with and without clinical signs of pathologies over several years. Determine differences in the immune status of fish with different signs of pathology based on the results of long-term studies. This will help to understand how UDN disease develops over time and what causes it.

Osmoregulation affects elimination of microplastics in fish in freshwater and marine environments.

In recent decades, microplastics (MPs) have emerged as one of the biggest environmental challenges in aquatic environments. Ingestion and toxicity of MPs in seawater (SW) and freshwater (FW) fish have been studied extensively both in field and laboratory settings. However, the basic mechanism of how fish deal with MPs in SW and FW remains unclear, although physiological conditions of fish differ significantly in the two environments. In this study, using Javanese medaka (Oryzias javanicus), a euryhaline fish that adapts readily to both SW and FW, we investigated elimination of MPs in fish in SW and FW environments. We exposed O. javanicus larvae (21days post-hatching) to 0.25mg/L of fluorescent polystyrene microspheres (1μm) for 24hours and then conducted an elimination test for up to 5days. Results showed that the gut retention time of MPs is longer in FW than in SW, indicating that MP elimination occurs more quickly in SW than in FW. However, higher numbers of MPs tended to be retained longer in SW larvae than FW larvae. Subsequently, using a fluorescent marker, gastrointestinal fluid was found to move more rapidly in the SW group. This finding indicates that water drinking accelerates gastrointestinal fluid movement, which moves MPs through the gut in SW larvae. Beside the difference in physiological conditions, MP elimination was faster when food was available, suggesting that feeding also affects MP elimination in fish. Internal factors such as body size and intestine length were also examined, but indicated no significant difference. Therefore, osmoregulation and feeding both influence MP elimination in fish.

Understanding the habitat status of fishes in the upper reaches of the Nogawa River , Tokyo: Combining sampling surveys and environmental DNA surveys.

Understanding the habitat status of fishes in the upper reaches of the Nogawa River , Tokyo: Combining sampling surveys and environmental DNA surveys.

A Study of the Toxic Effect of Polystyrene Microplastics on Oxygen Consumption in a Freshwater Fish Heteropneustes fossilis

Microplastics (MPs) are new emerging environmental pollutants in aquatic environments that attract widespread attention due to their small size and potential toxic impacts on aquatic organisms. In the present study, we investigated the toxic effects of short-term exposure to polystyrene microplastics at different doses on the oxygen consumption of Heteropneustes fossilis. The fish, after acclimatization, was exposed to polystyrene microplastics at 10.79µg/L, 17.98 µg/L, and 35.96 µg/L for 12 hours.The control group (first group) of fishes showed oxygen consumption of 72.86± 5.05 mg/kg/hr, the second group 69.21±3.64 mg/kg/hr, the third group 57.64±4.04, and the fourthgroup 51.92±4.95 in the closed chamber while 38.99 ± 2.34 mg/kg/hr, 33.47± 1.28 mg/kg/hr, 33.38± 2.40 mg/kg/hr, 29.36± 1.24 mg/kg/hr in open chamber. The decreasing trend of oxygen uptake in the fish was found in both cases after intoxication. The trend may be due to stress conditions of fish correlated with low RBC values and hemoglobin levels due to microplastic intoxication. Alterations in oxygen consumption may be due to respiratory distress due to impaired oxidative metabolism.

The Implementation of Internet of Things (IOT) for Aquaponic Cultivation

Aquaponic is a plant cultivation technique that is widely used by farmers and today’s communities due to its efficiency and ability to increase the agricultural productivity. The aquaponic cultivation in general still uses simple systems, such as manually feeding the fish by spreading the feed at predetermined times, monitoring water pH using a pH meter and monitoring water height or level through measurements, requiring farmers to spend time and special labor to care for and maintain plants and fish. Therefore, a solution is needed in the form of a system that can monitor and control plants and fish conditions automatically and continuously for 24 hours. The system should have the ability to control and monitor feeding activities, water pH, water and environmental temperature, water level and environmental humidity. The system in question is the internet of things (IoT) system that can be used as a tool for automatic control and monitoring through an application. The IoT system consists of several sensors that are connected to a microcontroller which can measure water pH, temperature, water level and environmental humidity. The data obtained by the sensor will be sent to a server via Wi-Fi protocol and stored in a database. The system is equipped with a web application that can be accessed through a computer device. The application provides a visual display of data: time, water pH, temperature, water level and environmental humidity, making it easier for farmers to monitor aquaponic conditions from a distance without having to come to the land. Through the implementation of IoT in aquaponic cultivation, farmers can increase efficiency and agricultural productivity by reducing the time, labor and costs required for control and monitoring.

Struktur Komunitas dan Status Konservasi Ikan Berdasarkan Alat Tangkap Tradisional di Desa Simonis Kabupaten Labuhan Batu Utara

Fish diversity must be maintained because it has an ecological role, namely maintaining the balance of the ecosystem and can be used as an indicator of pollution. diversity is also important for the balance of aquatic ecosystems and some simonis villagers make it a community livelihood. Based on information from residents of Simonis Village, North Labuhan Batu Regency, it is recognized that the diversity found in the river is quite high. In addition, residents of simonis village, north labuhan batu district can also utilize river water in pulo biski as drinking water, washing clothes, bathing and tourism. This study aims to determine the community structure, ecological index and conservation status of fish obtained in Simonis Village based on IUCN data. This research is a survey with the determination of observation stations through purposive sampling method. Fish sampling was carried out using environmentally friendly fishing gear such as nets, shoots, fishing rods and tanggok or nets. The results of this study obtained approximately 22 species of fish and each fish species and morphological identification was carried out in order to know the scientific name of each type of fish. Index value and Diversity with moderate category. Diversity Index value with high category. Dominance Index value with no dominance criteria. It is concluded that the diversity of fish in Simonis Village, North Port Batu Regency.

Appraisal of Fish Health Assessment Index in Three Major Reservoirs in Ekiti State, Southwest Nigeria

Increased anthropogenic activities and chemical contaminants in Ekiti State reservoirs have substantial effects on the reservoirs’ fish, which are valuable indicators of the health of the aquatic environments. The aim of the study was comparatively assessing the probable environmental deterioration in Egbe, Ero and Ureje reservoirs using the fish Health Assessment Index (HAI). Blood component analysis, necropsy data, and parasite index were used to compute HAI. Haematocrit values were not within the normal range in 78.85% (Ureje reservoir), 73.47% (Egbe reservoir), 57.38% (Ero reservoir), and endoparasite were present in 32.69%, 26.53% and 44.26% of fish examined from Ureje, Egbe, Ero reservoirs. Abnormalities of eyes, fins, skin, and liver were observed in a small proportion in the fish from the reservoirs. HAI of Oreochromis niloticus in the reservoirs was in order of Ureje reservoir (31.15 ± 2.81) > Egbe reservoir (29.39 ± 2.57) > Ero reservoir (25.00 ± 2.43). Condition factor of fish was in order of Ero (2.07 ± 0.03) > Egbe (1.98 ± 0.01) > Ureje (1.97 ± 0.01). In comparison to control site, reservoirs’ fish were in deplorable conditions as evidenced by HAI. Therefore, HAI is useful as an early indicator of the well-being of the aquatic ecosystems, in order to enhance the sustainability of the aquatic resources and also to compare the health status of fish from different aquatic environments.

A Novel Platform Featuring Nanomagnetic Ligand Fishing Based on Fixed-Orientation Immobilized Magnetic Beads for Screening Potential Cyclooxygenase-2 Inhibitors from Panax notoginseng Leaves.

A novel screening platform based on an Fe3O4@C@PDA-Ni2+@COX-2 ligand fishing combination with high-performance liquid chromatography-mass spectrometry was first designed, synthesized, and employed to screen and identify COX-2 inhibitors from Panax notoginseng leaves. The obtained magnetic nanoparticles exhibit outstanding preconcentration ability that allows for controlling the enzyme orientation to avoid enzyme active site blocking, conformational changes, or denaturing during immobilization. The as-prepared Fe3O4@C@PDA-Ni2+@COX-2 composite was carefully characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier-transform infrared spectrometry (FT-IR), Xray powder diffraction (XRD), thermal gravimetric analyzer (TGA), vibrating sample magnetometer (VSM), and Zeta potential analysis. The analytical parameters influencing the magnetic solid-phase fishing efficiency were optimized by univariate and multivariate methods (Box-Behnken design) by testing a positive control and celecoxib with active and inactive COX-2. Under the optimized ligand fishing conditions, twelve potential COX-2 inhibitors were screened and characterized in Panax notoginseng leaves. The results indicate that the proposed method provides a simple, feasible, selective, and effective platform for the efficient screening and identification of active compounds from Chinese herbal medicine. It has guiding significance for the synthesis and development of novel anti-inflammatory drugs, and provides a reference for the efficient discovery of anti-inflammatory drugs or lead compounds from the complex system of Chinese herbal medicine.

Enhancing Fish Passage Efficiency: Lessons from UHE Porto Primavera’s Fish Ladder

Dams severely affect aquatic environments and block the longitudinal migration of fish. In order to mitigate the negative effects generated by these developments, fish passes, or fishways, are implemented in dams with the purpose of restoring river connectivity and allowing the movement of migrants. Nevertheless, fishways in neotropical areas often face design and construction issues that can reduce their efficiency and selectively disadvantage species with limited swimming capabilities. This study analyzes how a fish ladder on the Paraná River influences the black armored catfish (Rhinelepis aspera), a benthic, long-distance migratory species important to commercial fisheries. A total of 200 individuals were PIT-tagged and monitored for four months. The results showed that although many fish successfully located the fishway, only a small portion (3.5%) managed to complete the ascent. The interaction between the hydraulic characteristics of the fishway and the fish condition factor played a significant role in ascent performance. Our findings underscore the importance of assessing fishway suitability for benthic neotropical species to support conservation efforts in the Upper Paraná River Basin. To improve passage rates for R. aspera, we recommend optimizing flow conditions by adjusting orifice and notch configurations, incorporating roughness elements, and modifying resting pool designs. These adaptations would reduce energy expenditure for ascending fish, enhancing fishway performance and contributing to the sustainability of migratory species in this region.

Parallel development of social behavior in biological and artificial fish

Our algorithmic understanding of vision has been revolutionized by a reverse engineering paradigm that involves building artificial systems that perform the same tasks as biological systems. Here, we extend this paradigm to social behavior. We embodied artificial neural networks in artificial fish and raised the artificial fish in virtual fish tanks that mimicked the rearing conditions of biological fish. When artificial fish had deep reinforcement learning and curiosity-derived rewards, they spontaneously developed fish-like social behaviors, including collective behavior and social preferences (favoring in-group over out-group members). The artificial fish also developed social behavior in naturalistic ocean worlds, showing that these embodied models generalize to real-world learning contexts. Thus, animal-like social behaviors can develop from generic learning algorithms (reinforcement learning and intrinsic motivation). Our study provides a foundation for reverse-engineering the development of social behavior using image-computable models from artificial intelligence, bridging the divide between high-dimensional sensory inputs and collective action.

Condition of Coral And Reef Fish in Four Diving Areas Frequently Visited During Marine Tourism in Bunaken National Park

Condition of Coral And Reef Fish in Four Diving Areas Frequently Visited During Marine Tourism in Bunaken National Park

Diet, feeding, and niche overlap of west coast steenbras (Lithognathus aureti) and silver kob (Argyrosomus inodorus) in the northern Benguela.

In this study, we described and compared the diet, monthly feeding intensity, and condition of west coast steenbras (Lithognathus aureti) and silver kob (Argyrosomus inodorus) caught at a unique habitat in the northern Benguela. Stomach contents of 179 west coast steenbras and 114 silver kob caught from October 2020 to September 2022 were investigated. The peak in feeding intensity of west coast steenbras appeared to be opportunistic during winter and summer periods depending on food availability. The fish condition, however, peaked at the beginning (October) and at the end (April) of the austral summer spawning period, with the hepatosomatic index (HSI) at 1.5% and the condition factor (CF) at 0.022%. Seven prey taxa were found in the diet of west coast steenbras (bivalves, bony fishes, other mollusks, algae, crustaceans, cnidaria, and polychaetas) and six taxa in the diet of silver kob (bivalves, crustaceans, bony fishes, algae, starfish, and zooplankton), indicating generalist feeding behavior in both the species. The bivalves were the most important prey items in the diet of west coast steenbras (95.9% index of relative importance [IRI]). The most important prey items in the diet of silver kob were crustaceans (83.1% IRI) and bony fishes (16.0% IRI). Crustaceans were most important in the diet of small-to-medium-sized silver kob, whereas bony fishes were most important in the diet of larger silver kob(>75 cm), with significant differences of IRI% by size class. Schoener's index of niche overlap indicated a relatively low overall niche overlap (0.11) between west coast steenbras and silver kob. This allows them to coexist as their feeding habits allow them to occupy unique niches in the coastal reef and sandy habitat and reduce competition for resources.

Effects of preserving juvenile rainbow trout (Oncorhynchus mykiss) in formalin and alcohol on body length, mass, and condition factor estimates

Abstract Body length and weight are key parameters measured to provide information about the growth and condition of larval, juvenile, and adult fishes from both the wild and aquaculture. The goal of this study was to evaluate the effects of preservation in formalin and alcohol on juvenile rainbow trout, Oncorhynchus mykiss (Walbaum) standard body length (SL) and weight changes. Standard length (19.43–32.98 mm SL) decreased after 60 days of preservation by an average of 2.98% in 96% alcohol and 2.26% in 10% formalin. In contrast, fish body weight (0.094–0.63 g) decreased in alcohol (31.22%) but increased in formalin (11.65%). These percentage values can be used as correction factors, with the exception of fish body weight in alcohol since the size of individuals affected the magnitude of change. Accordingly, a correction formula accounting for fish weight must be applied (fresh weight (g) = -0.0363 + 1.667 × preserved length). Changes in fish length and body weight in different preservatives resulted in significant differences in the Fulton condition factor, with underestimation in alcohol (fresh F = 1.58; preserved F = 1.20) and overestimation in formalin (fresh F = 1.60; preserved F = 1.92).

Ichthyofaunal Diversity and Seasonal Harvest Patterns in Dudhawa Reservoir, Chhattisgarh, India

This study seeks to examine the status and biodiversity of fishes in medium reservoir known as Dudhawa of Chhattisgarh. The major objectives were to collect the primary data on fisheries of Dudhawa reservoir, collection, preservation and identification of fish species, to enlist the fish fauna and to study the seasonal variations in fish harvest of Dudhawa reservoir. For this study, fish specimens were collected during August 2022 to February 2024 from the reservoir and primary data pertaining to species abundance, yield and production of the reservoir were collected from the Matsya Mahasangh Raipur. The results of empirical study revealed that the list of 43 species were classified under 5 orders, 13 families and 28 genera. Analysis of different species reveals that 69.76% of them belong to a single order, Cypriniformes. The analysis of the fishes listed show that 6.97 % species belong to order Mastacembeliformes, 11.62% belong to order Perciformes, 4.65% belong to order Channiformes and 2.32% species belong to order Cluipeiformes. Statistical analysis of data reveals no correlation between stocking and yield of the reservoir. Fish species in the Dudhawa reservoir were broadly categorized into four group i.e. major carps, catfishes, minor fish and minnow’s fish. Critical observation of data shows that major carp’s fishery dominated the catch during August, 2022 to February, 2024. A high yield of major carp’s fishery (26728 kg) was obtained in November. However, catfish fishery fluctuated between 2400.5 kg in August to 9063 kg in March. Bulk of minnows fishes (23.33%-56.6%) were recorded in drag netting may be due to reduced water level during summer (February to May, 2023). This data will definitely help the fishermen and as well as fisheries officials for better stock and harvest management of this type of small reservoir of Chhattisgarh.