Concomitant Variables Research Articles

An Explanation of a Gender Earnings Gap in the Veterinary Profession

Analysis of a unique data set of U.S. veterinary firms and veterinarians provides evidence of significant differences in earnings between self-employed males and females. After controlling for important concomitant variables, including training and experience, self-employed female veterinarians generally own smaller clinics and have lower earnings than their male counterparts. This study considers which demographic/economic factors may be associated with observed gender differences in earnings. The gender gap for average earnings is about 44 percent; however, when controlling for certain characteristics, the gap narrows to as little as 23 percent, depending on the model specification. Firm size was found to be the strongest contributing factor in explaining the gender gap in earnings, and so, possible explanations for differences in firm size have also been discussed. Whilst the analysis is not conclusive, there is evidence that self-employed females may face customer discrimination, which may constrain the growth and size of female-owned firms. (JEL D21) Keywords: Gender differences, salaries, self-employment, veterinarians

110 Pregnancy rate and embryo viability in response to chorionic gonadotrophins given for oestrus induction and gonadotrophin-releasing hormone 5 days after timed laparoscope-aided insemination of lactating and non-lactating goats

Gonadotrophins are included in oestrous/ovulation synchronization (E/OS) regimens when breeding early in the transitional phase to promote follicular development. However, the time of use has been a matter of debate. Hence, the objective of this study was to determine whether equine and human chorionic gonadotrophins (eCG and hCG, respectively) given 24h before or 24h after concurrent removal of intravaginal progestagen (P4) and prostaglandin (PGF2α) delivery influences pregnancy rate (PR) and/or embryo viability (EV). Relatedly, the influence of gonadotrophin-releasing hormone (GnRH) 5 days post-breeding (dpb) on PR and/or EV was also considered. A prospective randomised control trial was conducted using lactating and non-lactating Alpine breed goats of different parity, average±s.e. age, and bodyweight (3.7±2.6), and (52.5±7), respectively. Goats were time-bred at 48h after P4 removal by laparoscopy (LAI; n=75) or by natural service (NS; n=29) during the early transitional breeding phase in mid-September through mid-December (corresponding to 12:12h daylight/darkness). E/OS used an intravaginal device containing 300mg of P4 for 12±1d. At P4 removal, 2mL of PGF2α was administered IM. Twenty-four hours before (E) or 24h after (L) P4 removal, an IM dose of 1.5mL containing 120IU of eCG and 60IU of hCG was given. A third control (C) group (n=38) was not exposed to gonadotrophins. GnRH analogue was given (n=51) 5 days after insemination, and the remaining goats (n=53) received an IM dose of 2mL of saline solution. PR was evaluated at 18 to 24 (dpb) by non-return to oestrus (NRE), at 30 dpb by pregnancy-specific protein B (PSPB), and at 40 dpb by ultrasound imaging (UI). EV was determined from the difference between pregnancy outcomes for NRE and PSPB, and PSPB and UI. Statistical analysis (JMP/SAS vs.11; SAS Institute Inc.) were obtained using logistic regression. All concomitant variables (i.e. parity, bodyweight, days of P4 exposure, and lactation were not influenced by PR or EV; P>0.07). The overall PR was 71% for LAI and 69% for NS and, as shown in Table 1, was similar (P>0.49) at all times (at 18 to 24, 30, and 40 dpb). Likewise, the breeding procedure did not influence the levels of EV at 30 or 40 dpb. E/OS protocol or the use of GnRH 5 dpb did not affect PR or EV at any of the time points evaluated. Table 1. Main effect mean comparisons and logistic model probabilities for pregnancy rate (PR) and embryo viability (EV) by main treatment effect Response variable (days post-breeding) Breeding procedure E/OS Protocol Gonadotrophin LAI NS P> Control Early Late P> GnRH Placebo P> PR at 18–24 77 72 0.49 76 73 78 0.83 78 74 0.49 PR at 30 68 66 0.65 66 65 70 0.82 71 64 0.43 PR at 40 69 69 0.78 66 69 73 0.77 73 66 0.43 EV at 30 89 90 0.94 86 94 90 0.65 92 87 0.46 EV at 40 88 95 0.42 86 89 94 0.73 92 87 0.43

Fitting a Mixture Rasch Model to Visual Sequential Processing Memory Sub-dimension of ASIS: The Role of Covariates

Advanced Item Response Theory (IRT) practices serve well in understanding the nature of latent variables which have been subject to research in various disciplines. In the current study, 7-12 aged 2536 children’s responses to 20-item Visual Sequential Processing Memory (VSPM) sub-test of Anadolu-Sak Intelligence Scale (ASIS) were analyzed with Mixture Rasch Method (MRM). In the first phase of the study, concomitant (covariate) variables were not used. In the second phase, age and gender were added to the model, and then the two models were compared in terms of fit indices, the number of latent classes and the distribution of item difficulties in the latent classes. The results of the study suggested that there were three latent classes in both models; however, the latter model had greater fit compared to the former model. In addition, the latent classes in both models had similar characteristics, and the distributions of item difficulties in latent classes were also quite similar in both models while they had some differences in some aspects. The sizes of identical latent classes in both models varied between 15% and 30%. The results of the current study are expected to provide a deeper insight to researchers studying measurement theory and/or intelligence measurement.

112 Follicular dynamics and oestrus response of Alpine goats with oestrus/ovulation synchronized during the early transitional reproductive phase using gonadotrophin given early or late

Oestrus and ovulation synchronization (E/OS) regimens for fixed-timed breeding are useful when consistently eliciting ovulation. Early synchronization in the reproductive season promotes unreliable oestrus behavioural and physiologic response due to insufficient ovarian priming. In ruminants, equine (eCG) or human chorionic gonadotrophin (hCG) has FSH bioactivity or elicits ovulation, correspondingly. Hence, 120 and 60IU of eCG and hCG, respectively, are included in goat E/OS regimes. This experiment addresses the time when eCG/hCG (CG) is given relative to progestagen (P4) withdrawal and its effect on oestrus and ovulation. Fourteen non-lactating, Alpine breed goats, ranging from 1 to 6 kiddings, averaging±s.d. 3.9±2.0 years of age, and 56.3±5.0kg of bodyweight (BW), and body condition score (BCS) of 2.6±0.2, were evaluated early in the transitional reproductive phase (12h daylight:12h darkness). E/OS was accomplished with 12 day of P4 (200 mg) exposure by intravaginal insert. Three treatments were evaluated: Traditional (T; n=4) did not receive CG and served as the control group; early (E; n=5) received CG 24h before P4 removal; and reverse (R; n=5) received PGF2α 24h before P4 withdrawal as well as CG concurrent with P4 withdrawal. Oestrus response to E/OS was evaluated 24h after breeding using epididymectomized bucks. Ovaries were scanned by transrectal ultrasound (Aloka SSD-500V/7.5-MHz linear array probe) for 4 consecutive days starting with the first i.m. injection of 1.0mL of PGF2α or 1.5mL of CG. Images were digitized (MediCapture™) for later morphometry. The absence of effect (P>0.05) from concomitant variables age, BW, BCS, and parity was ascertained using a logistic regression model (JMP/SAS v15; SAS Institute Inc.). Ovulation, defined as the disappearance of the largest (>7mm) preovulatory follicle on a subsequent observation, was 100, 80, and 100%; and the average±s.e. number of ovulations per goat was 2±0.41, 1±0.32, and 1.3±0.49 (P>0.05). Table 1 summarises follicular size documented of 122 observations of ovulatory areas. Graafian follicle location was not different for left or right ovaries (P>0.05). Oestrus behavioural response to each E/OS treatment (T, E, or L) up to 24h after P4 removal was 50, 80, and 80% (P>0.05), respectively. In summary, oestrus response and ovulation were not affected by the variables studied. In this experience the timing of CG was not relevant. Table 1. Mean diameter (±SE; mm) of nonovulatory and preovulatory follicles on left (L) and right (R) ovaries E/OS protocol Non- and preovulatory Nonovulatory Preovulatory L R L R L R Traditional 6.4±0.6 5.5±0.6 4.8±0.4 4.5±0.4 7.7±0.4 8.9±0.3 Early 5.2±0.3 5.5±0.4 4.9±0.2 4.4±0.3 8.4±1.2 8.1±0.2 Reverse 6.6±0.5 5.3±0.6 5.1±0.4 4.4±0.5 8.7±0.3 8.1±0.2 Overall 5.7±0.19 4.7±0.14 8.3±0.14

Application of a Latent Transition Model to Estimate the Usual Prevalence of Dietary Patterns.

Background: This study aims to derive habitual dietary patterns of the Portuguese adult population by applying two methodological approaches: a latent class model and a latent transition model. The novel application of the latent transition model allows us to determine the day-to-day variability of diet and to calculate the usual prevalence of dietary patterns. Methods: Participants are from the National Food, Nutrition and Physical Activity Survey of the Portuguese population, 2015–2016 (2029 women; 1820 men, aged ≥18 years). Diet was collected by two 24 h dietary recalls (8–15 days apart). Dietary patterns were derived by: (1) a latent class model using the arithmetic mean of food weigh intake, with concomitant variables (age and sex); (2) a latent transition model allowing the transition from one pattern to another, with the same concomitant variables. Results: Six dietary patterns were identified by a latent class model. By using a latent transition model, three dietary patterns were identified: “In-transition to Western” (higher red meat and alcohol intake; followed by middle-aged men), “Western” (higher meats/eggs and energy-dense foods intake; followed by younger men), and “Traditional-Healthier” (higher intake of fruit, vegetables and fish, characteristic of older women). Most individuals followed the same pattern on both days, but around 26% transited between “In-transition to Western” and “Western”. The prevalence of the dietary patterns using a single recall day (40%, 27%, 33%, respectively) is different from the usual prevalence obtained by the latent transition probabilities (48%, 36%, 16%). Conclusion: Three dietary patterns, largely dependent on age and sex, were identified for the Portuguese adult population: “In-transition to Western” (48%), “Western” (36%), and “Traditional-Healthier” (16%), but 26% were transient between patterns. Dietary patterns are, in general, deviating from traditional habits.

Effects of adherence to pharmacological secondary prevention after acute myocardial infarction on health care costs \u2013 an analysis of real-world data

BackgroundAcute myocardial infarction (AMI), a major source of morbidity and mortality, is also associated with excess costs. Findings from previous studies were divergent regarding the effect on health care expenditure of adherence to guideline-recommended medication. However, gender-specific medication effectiveness, correlating the effectiveness of concomitant medication and variation in adherence over time, has not yet been considered.MethodsWe aim to measure the effect of adherence on health care expenditures stratified by gender from a third-party payer’s perspective in a sample of statutory insured Disease Management Program participants over a follow-up period of 3-years. In 3627 AMI patients, the proportion of days covered (PDC) for four guideline-recommended medications was calculated. A generalized additive mixed model was used, taking into account inter-individual effects (mean PDC rate) and intra-individual effects (deviation from the mean PDC rate).ResultsRegarding inter-individual effects, for both sexes only anti-platelet agents had a significant negative influence indicating that higher mean PDC rates lead to higher costs. With respect to intra-individual effects, for females higher deviations from the mean PDC rate for angiotensin-converting enzyme (ACE) inhibitors, anti-platelet agents, and statins were associated with higher costs. Furthermore, for males, an increasing positive deviation from the PDC mean increases costs for β-blockers and a negative deviation decreases costs. For anti-platelet agents, an increasing deviation from the PDC-mean slightly increases costs.ConclusionPositive and negative deviation from the mean PDC rate, independent of how high the mean was, usually negatively affect health care expenditures. Therefore, continuity in intake of guideline-recommended medication is important to save costs.

Improved Equations for the Density Management Diagram Isolines of Ponderosa Pine Stands

AbstractThis study was conducted to improve estimation of concomitant variables for implementation of a stand density management diagram (SDMD) for ponderosa pine (Pinus ponderosa Laws.) in northern California and Oregon. In traditional SDMD, isolines for variables such as stand volume are presented in such a way that uncertainty with estimation is not available. We developed the new top height and stand volume equations, as well as aboveground biomass and percent canopy cover, for building isolines in the SDMD using high-quality data collected from well-managed even-aged stands. The data were selected from the USDA Forest Service’s Pacific Southwest Research Station database. A total of 829 observations (from 113 plots across 15 sites in Oregon and California) were used for model construction. In addition, covariance-variance structures of all of the estimated parameters were provided so that users can evaluate the uncertainty associated with predictions. The model validation results indicated that the predictions made from fixed-effects model forms performed better than the current volume equation of SDMD, as well as those from mixed-effects model forms using the population average effect. The proposed equations provide enhanced predictions and additional useful information about managed ponderosa pine stands, including their uncertainty.

Lumbar bone mineral density measured by quantitative computed tomography (QCT): association with abdominal adipose tissue in different menopausal periods of Chinese women

Objective To investigate the correlation between abdominal adipose tissue and lumbar bone mineral density (BMD) in different menopausal periods of Chinese women. Methods 230 women were included in this cross-sectional study. Subjects were divided into a perimenopausal and postmenopausal group. Lumbar BMD was measured by QCT to assess total adipose tissue (TAT), visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT). The concomitant variables age, body mass index (BMI), and endocrine hormones were also considered. Multiple linear regression was used to assess the relationship between abdominal adipose tissue and BMD. Results In the perimenopausal group, Spearman correlation analysis showed that there was no significant association among TAT, SAT, VAT, and BMD (all p > .05). In the postmenopausal group, BMD was negatively correlated with TAT, SAT, and VAT. In both groups, after adjustment for age and BMI, multiple linear regression analysis showed that VAT was negatively correlated with BMD (p < .05). In contrast, there was no significant correlation with TAT, SAT, and BMD. Conclusions High VAT volume is associated with low lumbar BMD in both perimenopausal and postmenopausal women. TAT and SAT have no significant correlation with lumbar trabecular BMD.

Computed Tomography Angiography-Based Evaluation of Anatomical Variations of the Celiac ‎Trunk and Renal Arteries

Background: The abdominal aorta and its main branches, such as the celiac trunk and the renal arteries are manipulated during various radiologic, surgical, and oncologic procedures. This study aimed at evaluating the anatomical pattern of these vessels to assist surgeons and radiologists reduce the risk of intra- as well as postoperative complications.&#x0D; Methods: A retrospective analysis of 536 Computed Tomography Angiography (CTA) studies of living potential kidney donors was conducted from January 2012 to December 2018.&#x0D; Results: The anatomical variations of the celiac trunk was found in 9.5% of the cases. Among these cases, the most frequent variation was the Left Gastric Artery (LGA) as the first branch of the celiac trunk (80.4% of the cases). Gender was not overall significantly associated with the variations of the celiac trunk (P=0.670); however, there was a significant correlation between male gender and the most prevalent form of the celiac trunk variation (P=0.004). Variations of the renal artery occurred in 22.94% of the cases, with the left accessory renal artery being the most common variant (28.45% of the cases). Gender and the involved side (right/left) were not significantly related to the renal artery variations (P=1.000 &amp; P=0.546, respectively). No concomitant variation of the celiac trunk and the renal artery was detected in our study.&#x0D; Conclusion: The anatomical variations of the celiac trunk and the renal arteries occur commonly; thus, the branching pattern of these arteries should be assessed prior to any procedure concerning them.

Non-inverted U-shaped challenges to regional sustainability: The health risk of soil heavy metals in coastal China

Heavy metal pollution has become a significant cause for concern in various fields. However, there is little research available on the relationship between economic development and the health effects of soil heavy metal pollution. We extracted soil heavy metal exposure data (including data on Cd, As, Hg, Pb, and Cr) for 364 counties and 106 prefecture-level cities in the coastal development areas of China from literature published between 2013 and 2018. Using this dataset, we aimed to analyze the spatial distribution of soil heavy metals and their related health risks and explore the relationship between socioeconomic factors and health risks. The results showed that Cd was the dominant soil heavy metal in the coastal development areas of China, and significant positive synergistic relationships were found between Pb and Cr, Hg and As, Hg and Cd, and Cd and As. Although the non-carcinogenic risk and carcinogenic risk induced by the soil heavy metals were low, the overall health risk exhibited a positive synergistic relationship with secondary industry after controlling for concomitant variables. Unlike the concentration trends of water and air pollutants, which show an inverted U-shaped relationship with economic growth, the health risks associated with soil heavy metal pollution continued to increase with economic growth. These health risks increased more rapidly after gross domestic product reached a certain level, indicating the importance of reducing heavy metal pollution during periods of economic development and transition.

Experimental control of the degree of non-classicality via quantum coherence

The origin of non-classicality in physical systems and its connection to distinctly quantum features such as entanglement and coherence is a central question in quantum physics. This work analyses this question theoretically and experimentally, linking quantitatively non-classicality with quantum coherence. On the theoretical front, we show when the coherence of an observable is linearly related to the degree of violation of the Kolmogorov condition, which quantifies the deviation from any classical (non-invasive) explanation of the multi-time statistics. Experimentally, we probe this connection between coherence and non-classicality in a time-multiplexed optical quantum walk. We demonstrate exquisite control of quantum coherence of the walker by varying the degree of coherent superposition effected by the coin, and we show a concomitant variation in the degree of non-classicality of the walker statistics, which can be accessed directly by virtue of the unprecedented control on the measurement-induced effects obtained via fast programmable electro-optic modulators.

Researching unexplained phenomena: empirical-statistical validity and reliability of the Multivariable Multiaxial Suggestibility Inventory-2 (MMSI-2)

Anomalous phenomena are unexplained occurrences, such as paranormal experiences, that challenge the ontological bases of current scientific knowledge and are considered scientifically impossible. Problematically, some scientific research yields significant statistical results in favor of the existence of telepathy, precognition, mind-matter interaction, and mediumship. The current study presents and statistically justifies the Multivariable Multiaxial Suggestibility Inventory-2 (MMSI-2), a new psychological instrument to measure and detect the main psychological explanations for anomalous experiences. A nonprobabilistic sample of 3,224 subjects without a psychiatric history were recruited from the general population of Spain. Exploratory factor analysis (EFA) was used to examine the internal structure of the MMSI-2's 174 items. Direct oblimin and promax oblique rotations were applied as criteria for axis rotation. Cronbach's alpha coefficients and their ordinal transformation were also calculated, and gender-differentiated scales for the raw MMSI-2 scale scores were developed. The first-order factorial solution yielded a total of 16 factors that explained 92.84% of the variance. Of these, 10 corresponded to the psychological variables cited in the background literature, four classified the anomalous phenomena according to their sensory mode, and two represented prototype control scales for this class of psychometric inventory. The higher-order EFA grouped the MMSI-2 scales into four macrofactors that together explained 97.737% of the variance. Satisfactory reliability rates were obtained (alphas>0.8). The full version of the MMSI-2 with 174 items is a valid and reliable psychometric instrument for evaluating anomalous phenomena and the theoretically concomitant psychological variables. Similarly, the scaling of scores can be used in psychological assessment as a screening tool to identify clinically suspected psychological variables.

Thyroglobulin Changes are Highly Dependent on TSH in Low-risk DTC Patients not Treated with Radioiodine

Low-risk differentiated thyroid cancer (DTC) is currently rarely treated with radioiodine (131I) to ablate the postoperative remnant. Therefore, the interpretation of the serum thyroglobulin (Tg) values should be reconsidered. The aim of our study was to evaluate the changes in Tg values during follow-up with regard to the changing values in thyroid stimulating hormone (TSH). We evaluated 271 low-risk DTC patients, treated with total thyroidectomy but not 131I. To be included, patients had to be negative for Tg antibodies and have at least 3 evaluations in our department. All patients were on levothyroxine (L-T4) therapy. After a median follow-up of 73 months, the overall Tg values were stable, while TSH values slightly increased. Therefore, we pooled data of Tg and TSH from all evaluations and a significant positive correlation was demonstrated (R = 0.2; P < 0.01), and was also demonstrated when we performed the analysis using time-weighted values (R = 0.14; P = 0.02). Moreover, when dividing patients into 3 groups according to first postoperative Tg (Group A [Tg < 0.2 ng/ml], Group B [Tg 0.2-1 ng/ml], and Group C [Tg > 1 ng/ml]) most patients showed stable values of Tg at the end of follow-up but TSH variations had a clear impact on the changes in Tg among the groups. We demonstrated that in low-risk DTC not treated with 131I, serum Tg remains substantially stable over time, and the variations observed were correlated with the concomitant variations of TSH levels, mainly due to the modification of LT-4 therapy performed according to the ongoing risk stratification.

Contribution of DNA damage repair gene mutations and concomitant TP53 variations to the efficacy of platinum-based chemotherapy as initial therapeutic option in Chinese NSCLC patients.

e21002 Background: Though targeted, immune-, even combinational therapies have been applied widely, platinum-based chemotherapy still plays irreplaceable role as initial management for patient with either resected or advanced NSCLC without actionable target. Platinum is sought to increase DNA damage burden inducing cell death, especially in cells with intrinsic DNA damage repair (DDR) dysfunction. This study aims to explore impact of DDR genes as well as co-occurrence of TP53 mutations on prognosis of NSCLC patients. Methods: 110 NSCLC patients were consecutively recruited from 2016-2018. 508 cancer related genes were sequenced by BGI-seq 500. COX regression was used to evaluate risk of disease progression as well as cancer-related death among patients with various DDR/TP53 status. Results: 61 NSCLC patients with stage II-IV (median age of onset: 55 years) were recruited who received platinum-based therapy as adjuvant or 1st line systemic therapy. Till 15th.Oct.2019, median follow-up time was 24 months. 21 patients (34.4% of 61) carried DDR mutations. TP53 mutations were detected in 36 patients (59% of 61). 11 patients (18% of 61) displayed TP53/DDR co-mutations. COX regression analysis indicated NSCLC patients with DDR mutation would have lower risk of disease progression (HR:0.37, P = 0.19) as well as superior overall survival time (HR:0.35, P = 0.035). Patients with DDR co-mutations had no superior survival benefit. Patients with HRR mutations presented similar result. Patients with TP53 mutations had similar risk as ones with other mutations. While those with DDR concomitant mutations showed significantly improved clinical benefit (HR for PFS:0.28, P = 0.05; HR for OS:0.22, P = 0.023). Conclusions: DNA damage repair genes in Chinese NSCLC patients may play vital roles in predicting efficacy of platinum-based chemotherapy even in the context of TP53 co-mutation. Further, combination of BRCA2 and PALB2 may be better associated with patients’ prognosis. Large cohort should be set up to validate this finding in near future. [Table: see text]

Trehalose Effect on the Aggregation of Model Proteins into Amyloid Fibrils.

Protein aggregation into amyloid fibrils is a phenomenon that attracts attention from a wide and composite part of the scientific community. Indeed, the presence of mature fibrils is associated with several neurodegenerative diseases, and in addition these supramolecular aggregates are considered promising self-assembling nanomaterials. In this framework, investigation on the effect of cosolutes on protein propensity to aggregate into fibrils is receiving growing interest, and new insights on this aspect might represent valuable steps towards comprehension of highly complex biological processes. In this work we studied the influence exerted by the osmolyte trehalose on fibrillation of two model proteins, that is, lysozyme and insulin, investigated during concomitant variation of the solution ionic strength due to NaCl. In order to monitor both secondary structures and the overall tridimensional conformations, we have performed UV spectroscopy measurements with Congo Red, Circular Dichroism, and synchrotron Small Angle X-ray Scattering. For both proteins we describe the effect of trehalose in changing the fibrillation pattern and, as main result, we observe that ionic strength in solution is a key factor in determining trehalose efficiency in slowing down or blocking protein fibrillation. Ionic strength reveals to be a competitive element with respect to trehalose, being able to counteract its inhibiting effects toward amyloidogenesis. Reported data highlight the importance of combining studies carried out on cosolutes with valuation of other physiological parameters that may affect the aggregation process. Also, the obtained experimental results allow to hypothesize a plausible mechanism adopted by the osmolyte to preserve protein surface and prevent protein fibrillation.

Changes in the levels of adipokines in newly diagnosed pulmonary tuberculosis patients in case of treatment failure

Improving the treatment efficacy for patients with newly diagnosed tuberculosis by reducing the number of treatment failure cases is very urgent issue nowadays. Purpose. To define the characteristics of adipokines levels changes in newly diagnosed pulmonary tuberculosis patients in case of treatment failure by examining the levels of leptin, adiponectin and leptin/adiponectin ratio. Materials and methods . A total of 58 patients with newly diagnosed pulmonary tuberculosis were the subject of this prospective study. The patients were divided into 2 groups: the first group was made up of 28 patients with the ineffectively treated newly diagnosed pulmonary tuberculosis; the second group (experimental group) consisted of 30 patients with successful treatment completion. The control group included 29 healthy voluntary donors. Changes in the levels of adipokines by measuring the blood serum leptin and adiponectin via enzyme linked immunosorbent assay using immunoassay analyzer Sirio S with “Mediagnost” kit (Germany) (ng/ml) were studied at the Scientific Medical Laboratory Center of Zaporizhzhia State Medical University. Leptin/adiponectin ratio was calculated mathematically (relative units). Blood samples were collected after an overnight fasting. Statistica for Windows 13.0 (Copyright 1984–2018 TIBCO Software Inc., all rights reserved, Licence No. JPZ8041382130ARCN10-J) software was used for the statistical analysis. Results. The following significant changes in the adipokines levels have been found in patients with newly diagnosed pulmonary tuberculosis with massive pulmonary lesions and destructions of over 3 сm in diameter and positive sputum smear. These patients will be diagnosed with ineffective treatment. At the beginning of the antimycobacterial therapy, there was a significant 12.7 times decrease in leptin and 1.4 times increase in adiponectin indicators and 21 times decrease in the leptin/adiponectin ratio. The levels of leptin, adiponectin and leptin/adiponectin ratio were significantly worse than similar levels in patients, who completed the treatment efficiently. The level of the leptin was slightly increased during the antimycobacterial therapy, albeit being significantly reduced, and the level of the adiponectin continued to increase significantly. The leptin/adiponectin ratio was significantly lower than that in patients who completed the treatment efficiently. Conclusions. Patients with newly diagnosed pulmonary tuberculosis with the risk of treatment failure are diagnosed with severe disorders of adipocyte functional activity, that antimycobacterial chemotherapy could not even restore the balance to the adipokine system and smooth down resulting concomitant variations in immune response. The findings showed the prognostic value of the changes in leptin, adiponectin and leptin/adiponectin ratio indicators at the beginning of the treatment in relation to treatment failure among patients with newly diagnosed pulmonary tuberculosis.

Response of methane emissions to litter input manipulation in a temperate freshwater marsh, Northeast China

The photograph of the field experiment of different litter input manipulations in situ and the treatment effects on CH 4 fluxes during observations in 2015 and 2016. Abbreviations: NL, no aboveground litter; DL, double litter; CK, control; AR, standing litter removal. • We conducted a two seasons litter input manipulation experiment in a freshwater marsh. • Litter input manipulations obviously affected CH 4 emission and surface DOC contents. • Litter removal induced higher soil temperature, DOC content and CH 4 emission. • The abundance of methanogens in surface soil relied on the ways of litter manipulation. Plant litter plays an important role in regulating CH 4 emission in wetland ecosystems. Our knowledge of the impacts of plant litter on CH 4 budget is important for understanding the response and feedbacks of wetland ecosystems to climate warming. Nevertheless, the effects of plant litter on CH 4 emission and temperature sensitivity (Q 10 ) are not clear under the background of global change. In this study, a field litter inputs manipulation experiment with four treatments including no aboveground litter (NL), standing litter removal (AR), doubled litter input (DL) and controls (CK) was conducted to study the effects of litter manipulations on CH 4 emission in a seasonal inundated freshwater marsh over two growing seasons of 2015 and 2016 in the Sanjiang Plain, Northeast China. Results showed that CH 4 emission was significantly associated with altered litter input over the observation period. Compared with CK, the cumulative CH 4 emission increased by 105.83%, 42.20% and 42.50% for NL, AR and DL treatments, respectively, in 2015, and the values were 199.04%, 115.55% and 17.55%, in 2016. Variances of CH 4 emission were more sensitive to litter removal effects. Altered litter input also significantly affected the dissolved organic carbon (DOC) concentration ([DOC]) in surface pore water. DL and CK treatments with lower soil temperature had lower [DOC] than litter removal treatments of NL and AR. The abundance of methanogens among the four treatments exhibited synchronous change with DOC over the observation period. The Q 10 values of CH 4 emission didn’t show consistent changing patterns with those of DOC among different litter input treatments, which was probably due to the different temperature sensitivity of dominant methanogen types. Due to its great effects on soil microclimate, substrate availability and the concomitant variation in temperature sensitivity, our results highlighted that plant litter effects should be incorporated into the current process-based CH 4 biogeochemical models to predict future CH 4 fluxes from natural wetlands under the background of global change.

Morphological traits correlated with resource partitioning among small characin fish species coexisting in a Neotropical river

AbstractBody shape variation should play a central role in determining the feeding ability of a fish species. We tested whether variations in feeding resource use of sympatric and closely related fish species could be explained by differences in ecologically relevant metrics, such as body morphology, trophic apparatus or combinations thereof. Fish were sampled at 14 sampling sites from Pelotas River and tributaries, Upper Uruguay, Brazil. The stomach contents of five species of Characidae (Astyanax and Bryconamericus) were analysed by the volumetric method. A geometric morphometric approach was used together with internal morphological traits to predict their patterns of prey use. Significant differences in body depth, snout and caudal peduncle lengths were found. Concomitant pronounced variations were verified to occur among species in structures related to digestion and absorption. Increased numbers of cusps, more pyloric caeca and higher intestinal coefficient values were observed in species that consumed greater proportions of plants. The opposite trends occurred in species that consumed greater proportions of insects. Mixed linear models indicated that the characin species differed significantly in the use of algae, aquatic insects, terrestrial insects, plant remains/seeds and detritus. Dietary differences reflected in low trophic niche overlap. Partial least squares regression results indicated strong correlations between morphological divergences and the corresponding variations in species diet. These outcomes combined confirm that the morphological traits that best enables the exploration of certain resource were an important factor in the resource‐related divergence and were the underlying reasons for these species' respective feeding modes.

The Marital Implications of Bereavement: Child Death and Intimate Partner Violence in West and Central Africa.

In high-mortality contexts, research examining the effects of child mortality has focused almost exclusively on couples' fertility responses while overlooking other potential family consequences. Using nationally representative survey data from 13 West and Central African countries, we estimate multilevel discrete-time hazard models to determine how women's risk of intimate partner violence (IPV) varies with the death of children. We assess heterogeneity in this association across two surrounding circumstances: children's age at death and regional prevalence of child bereavement. Findings indicate that the risk of IPV initiation rises with the death of children under age 5-for whom women are most intensely responsible-but not with the death of older children. The effect of young child bereavement is most pronounced in regions where it is least prevalent among mothers-a finding not explained by concomitant regional variation in gender inequality, family norms, and infrastructural development. These findings highlight the importance of child mortality for family outcomes beyond fertility in the African context and demonstrate the prominent role of the broader mortality context in shaping these implications.

The association between metal exposure and semen quality in Chinese males: The mediating effect of androgens

As a crucial factor in male reproduction, androgens may represent an intermediate biological mechanism linking metal exposure with effects on semen quality. This study aimed to investigate the association between metal exposure and semen quality, and to assess the mediating role of seminal androgens between metal exposure and semen quality. We investigated the presence of 10 metals in semen and assessed their effect on semen quality in 1136 men recruited from a hospital in Shenzhen, China. Of these, 464 subjects were randomly selected for 4 androgens detection in semen. Cross-sectional associations between single/multiple metals, androgen levels and semen quality were explored by multivariable linear regressions. Mediation analysis was performed to detect the role of seminal androgens on the association between metal exposure and semen quality. Seminal selenium and iron were positively associated with both sperm concentration and total sperm count. Negative associations were observed between both manganese and zinc and sperm concentration, molybdenum and total sperm count, copper and sperm motility. Furthermore, we found significant dose-dependent relationships between both iron and selenium levels and dihydrotestosterone (DHT), arsenic levels and testosterone, as well as zinc and dehydroepiandrosterone. Mediation analysis indicated that higher seminal iron and selenium were associated with an increasing sperm concentration after controlling for DHT, with 10.32% and 12.89% of these associations were mediated by DHT, respectively. A similar mediation effect of DHT was observed in the associations between iron and selenium levels and total sperm count (13.39% and 21.57% mediation, respectively). Our findings suggested that the presence of selenium and iron in semen was beneficial to sperm concentration and total count. Seminal manganese, zinc, molybdenum and copper may be associated with reduced semen quality. The associations between seminal selenium and iron and sperm concentration and total count were partially explained by the concomitant variation of seminal DHT.