Objective: The objective of this research was to analyze the applicability of biotechnology as a tool for sustainability and innovation in industry 4.0. Methodology: The methodology adopted consisted of a bibliographic survey carried out on academic platforms such as SciELO, Scopus and Google Scholar, in addition to Brazilian repositories such as CAPES and BDTD. The research included skimming and an analysis of selected materials, addressing both conceptual and practical aspects of biotechnology in industry. Results and data analysis: The results revealed the evolution of the relationship between human beings and the environment, highlighting the growing awareness of sustainability and the importance of urgent actions. Industry 4.0, in turn, represents the integration of advanced technologies that transform factories into connected and efficient environments, focusing on automation, big data and personalization. Biotechnology has proven essential, using living organisms to develop sustainable products and processes, with significant advances in the areas of health, agriculture and environmental preservation. The intersection between biotechnology and Industry 4.0 allows for the implementation of more sustainable agricultural practices, personalized medical treatments, biofuel production and bioremediation, although it also raises ethical and safety concerns. The conclusion highlights that biotechnology plays a crucial role in promoting sustainability and innovation, offering solutions that optimize processes and reduce environmental impacts. However, challenges such as regulations and ethical issues need to be addressed. Collaboration between governments, industries and research institutions is vital to maximize the benefits and ensure a safe and responsible application of these technologies, thus being able to positively influence the future of the industry towards a more sustainable and efficient production model. Final considerations: In conclusion, the research revealed that the intersection between biotechnology and Industry 4.0 is fundamental to promoting sustainability and innovation. Biotechnology offers sustainable solutions in areas such as healthcare, agriculture and environmental preservation, while Industry 4.0 enables optimization and efficiency through digitalization and automation. However, ethical and regulatory challenges must be addressed. Collaboration between governments, industries and research institutions is crucial to ensure the safe and responsible application of these technologies, shaping a more sustainable and efficient industrial future.
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