Conceptual Advances Research Articles

Remaking the Past: Identifying Sramanic Traditions from Kerala

For historians and researchers, reconstructing the glorious but hidden Jaina and Buddhist antiquity of the Kerala region has proven to be a great challenge in the absence of necessary sources. But its influence in Kerala society is unquestionable. A wide range of socio-economic, political, and artistic endeavours expanded heterodox faiths like Jainism and Buddhism throughout centuries and across numerous geographical regions, from the mid-Gangetic valley to Kerala. The contributions of the Jains and Buddhists to the development of society and culture in Kerala have not been adequately highlighted and integrated into the mainstream of Kerala historical studies, despite the empirical and conceptual advances made in Kerala historiography over the last two decades. The history of the Jains and the Buddhists was pushed to the back burner in favour of the major focus of Kerala history and culture, centring on Brahmin settlements and temple-oriented sociocultural structures. To understand the socio-economic aspects of the spread of the heterodox Jain and Buddhist religio-cultural groups to Kerala, my article suggests setting a few epigraphical examples from the Jain and Buddhist relics in Kerala against the backdrop of Kerala's medieval history.

Introducing UV–visible spectroscopy at high school level following the historical evolution of spectroscopic instruments: a proposal for chemistry teachers

Spectroscopy is a scientific topic at the interface between Chemistry and Physics, which is taught at high school level in relation with its fundamental applications in Analytical Chemistry. In the first part of the paper, the topic of spectroscopy is analyzed having in mind the well-known Johnstone’s triangle of chemistry education, putting in evidence the way spectroscopy is usually taught at the three levels of chemical knowledge: macroscopic/phenomenological, sub-microscopic/molecular and symbolic ones. Among these three levels, following Johnstone’s recommendations the macroscopic one is the most useful for high school students who learn spectroscopy for the first time. Starting from these premises, in the second part of the paper, we propose a didactic sequence which is inspired by the historical evolution of spectroscopic instruments from the first spectroscopes invented by Gustav Kirchhoff and Robert Bunsen in 1860 to the UV–vis spectrophotometers which became common since the 1960s. The idea behind our research is to analyze the conceptual advancements through the history of spectroscopy and to identify the key episodes/experiments and spectroscopic instruments. For each of them, a didactic activity, typically an experiment, is then proposed underlining the relevant aspects from the chemistry education point of view. The present paper is the occasion to reflect on the potentialities of an historical approach combined with a laboratorial one, and to discuss the role of historical instruments and related technological improvements to teach spectroscopy.

New Vision of Cell Walls in Aspergillus fumigatus from Solid-State NMR Spectroscopy.

The fungal cell wall plays a critical role in regulating cellular integrity and communication, and serves as a frontline defense against stress. It is also a prime target for the development of antifungal agents. The cell wall is comprised of diverse polysaccharides and proteins and poses a challenging target for high-resolution structural characterization. Recently, the solid-state nuclear magnetic resonance (ssNMR) analysis of intact Aspergillus fumigatus cells has provided atomic-level insights into the structural polymorphism and functional assembly principles of carbohydrate components within the cell wall. This physical perspective, alongside structural information from biochemical assays, offers a renewed understanding of the cell wall as a highly complex and dynamic organelle. Here, we summarize key conceptual advancements in the structural elucidation of A. fumigatus mycelial and conidial cell walls and their responses to stressors. We also highlight underexplored areas and discuss the opportunities facilitated by technical advancements in ssNMR spectroscopy.

How and why did the transition process towards a fully sustainable production of cocoa beans in Bahia (Brazil) take place?

Abstract This paper theoretically models and empirically assesses the sociotechnical transition process experienced by a group of producers in southern Bahia State (Brazil). Driven by the search for economic sustainability in cocoa production, this group has achieved full sustainability through production chain upgrading, enabling the agroforestry system’s (cabruca) continuity in their properties. In order to understand how and why this process happened, our conceptual model advances on the Multi-Level Perspective (MLP) framework, as it is an applied way of analyzing different niche transformations within the same regime, a typical situation in agrifood systems in emerging markets. Our analyses and empirical evidence suggest that interpersonal relationships play a crucial role in this process, particularly developing concepts and strategies, and in establishing operational structures. Rules and structure formalization may replace interpersonal relationships, as observed when the transition goes forward. Our findings evidence knowledge gathering, cooperation among actors, and information and support exchanging as critical factors to trigger the niche transition process.

Practitioners' ability to retool the economy: The role of agency in local economic resilience to plant closures in Ontario

AbstractEconomic resilience focuses on how localities react and respond to shocks. A recent conceptual advancement, known as the agency perspective and rooted in evolutionary thinking, highlights that economic actors (e.g., practitioners, firms, and institutions) play an essential role in localities' resilience. However, empirical investigations into the role of economic actors have been scarce. To address this shortcoming, in this study we conducted in‐depth interviews with 22 practitioners (economic development officials) from various cities in Ontario, Canada. Through an evolutionary lens, we examine practitioners' perceptions of and responses to endogenous shocks—notably, major industrial plant closures over the past 20 years. We find that practitioners influence localities' resilience through each dimension of the resilience process. Most notably, they support their localities' adaption through various short‐ and long‐term adaptive strategies. Also, we find that economic actors have different capacities and resources at their disposal to respond to shocks depending on the size of their city and its geographical location. Regarding the latter, we reiterate a commonly noted north‐south divide in the province. In addition, we find that economic actors are constrained by multi‐scalar policies, and thus operate in the confines of existing power and political structures.

Dissecting Mechanisms of Epigenetic Memory Through Computational Modeling.

Understanding the mechanistic basis of epigenetic memory has proven to be a difficult task due to the underlying complexity of the systems involved in its establishment and maintenance. Here, we review the role of computational modeling in helping to unlock this complexity, allowing the dissection of intricate feedback dynamics. We focus on three forms of epigenetic memory encoded in gene regulatory networks, DNA methylation, and histone modifications and discuss the important advantages offered by plant systems in their dissection. We summarize the main modeling approaches involved and highlight the principal conceptual advances that the modeling has enabled through iterative cycles of predictive modeling and experiments. Lastly, we discuss remaining gaps in our understanding and how intertwined theory and experimental approaches might help in their resolution.

Distributional ecology: Opening new research windows by addressing aggregation‐related puzzles

AbstractAimDistribution of species is one of the most elementary but fundamental biodiversity patterns, yet too many puzzles remain unsolved. In most cases, distribution of species is not random, but presents some degree of aggregation. Accordingly, the term ‘distributional aggregation’ is prevailingly used in ecology and evolutionary biology to reflect the non‐random distribution characteristic of species in space and time. However, its meanings are multiform and can be decomposed into a variety of components.LocationGlobal.MethodsIn this paper, through synthesizing historical literature and comparing relevant meanings of distributional aggregation under different contexts, we summarize the current statistical metrics in detecting and evaluating distributional aggregation that are suitable for different field‐survey methods, study models and sampling scales. In particular, we explore the concept under the multi‐species setting for which few conceptual advances and statistical methods have been developed.ResultsWe propose pure data dispersion and spatiotemporal proximity, as two basic components of distributional aggregation. We further explore three advanced components of distributional aggregation: orthogonal, hierarchical and parallel components that can simultaneously link sampling taxa, sampling sites and sampling methods.Main ConclusionsWe hope the systematic review can serve as a potentially useful primer to ecologists for better understanding and investigating complex and new distributional patterns of biological diversity. We further provide informative guides on developing new statistical methods and metrics. We also discuss useful simulation algorithms for generating diverse distributional aggregation patterns of species, aiming to help ecologists to test and compare the performance of different metrics related to diversity and distribution patterns of species.

Defining a ‘Just Energy Investment’ for the ASEAN Just Transition

Governments across the world have been updating their energy and climate policies since the 2015 Paris Agreement. One common feature is the advent of long-term plans for a net-zero economy for 2050 and also in some cases to 2060. To meet these long-term net-zero targets therefore, significant investment in new and clean energy infrastructure is needed. This research focuses on how to ensure that this required energy investment happens in a ‘just’ way, i.e., it contributes to fairness, equity, equality and inclusiveness. Recently, countries such as the United States, South Africa and Colombia have incorporated justice principles into their energy and climate policies. This research builds on this and is the first to assess justice in consideration of these time horizons along with its focus on the 10 South-East Asian nations, known as ASEAN countries. The data analysed here provides insight into how energy investments are developing in these countries, whether justice policies are aligned and therefore how to avoid energy policy failure. Further, as well as the conceptual advances, this research provides a model for a Just Energy Investment (JEI) Index based on the five energy justice principles. The analysis here focuses on countries of the Global South where there exists limited research that looks at the performance over time of the combined issue of energy investments and justice; this demonstrates this research as a significant contribution to the literature. This research marks a first step in delivering justice in new energy development as these economies build towards their 2050 and 2060 energy and climate targets.

The Subjective Experience of Autobiographical Remembering: Conceptual and Methodological Advances and Challenges.

The investigation of the phenomenology of autobiographical memories (i.e., how a memory is subjectively experienced and its meaning) has provided an important contribution to our understanding of autobiographical remembering. Over the last two decades, the study of phenomenology has received widespread scientific attention, and the field has undergone quite relevant conceptual and methodological changes. In the present work, we (1) review some basic and well-established research findings and methodological achievements; (2) discuss new theoretical and methodological challenges, with a special focus on the issue of the phenomenological experience of the retrieval process and its relationship with the phenomenology of the products of retrieval; and (3) propose an alternative way of conceptualizing and understanding it in the framework of experimental phenomenology.

The human microbiome: 340 years of history, 140 years of interrogations, technological innovations and emergence of "microbial medicine"

For 350 years, we have known that the human body hosts microbes, then called "animalcules". For over a century, following the demonstration of the role of some of these microbes in diseases, questions have arisen about the role of the largely predominant ones colonizing human skin and mucous surfaces, particularly the rich microbial ecosystem of the intestine, the gut microbiota. From the invention of germ-free life - axenism - which experimentally validated the human-microbe symbiosis, resulting from a long coevolution, to the development of anaerobic culture methods, then to the invention of molecular diagnosis, deep sequencing opening up metagenomic and omics approaches in general, a remarkable race has taken place between technological innovations and conceptual advances. This race, beyond the exhaustive description of the microbiota in its intra- and inter-human diversity, and the essential symbiotic functions of the microbiome, has paved the way for a new field of medicine: microbial medicine.

Prospective fecal microbiomic biomarkers for chronic wasting disease.

Chronic wasting disease (CWD) is a naturally occurring prion disease in cervids that has been rapidly proliferating in the United States. Here, we investigated a potential link between CWD infection and gut microbiome by analyzing 50 fecal samples obtained from CWD-positive animals of different sexes from various regions in the USA compared to 50 CWD-negative controls using high throughput sequencing of the 16S ribosomal RNA and targeted metabolomics. Our analysis reveals promising trends in the gut microbiota that could potentially be CWD-dependent, including several bacterial taxa at each rank level, as well as taxa pairs, that can differentiate between CWD-negative and CWD-positive deer. Through machine-learning, these taxa and taxa pairs at each rank level could facilitate identification of around 70% of both the CWD-negative and the CWD-positive samples. Our results provide a potential tool for diagnostics and surveillance of CWD in the wild, as well as conceptual advances in our understanding of the disease.IMPORTANCEThis is a comprehensive study that tests the connection between the composition of the gut microbiome in deer in response to chronic wasting disease (CWD). We analyzed 50 fecal samples obtained from CWD-positive animals compared to 50 CWD-negative controls to identify CWD-dependent changes in the gut microbiome, matched with the analysis of fecal metabolites. Our results show promising trends suggesting that fecal microbial composition can directly correspond to CWD disease status. These results point to the microbial composition of the feces as a potential tool for diagnostics and surveillance of CWD in the wild, including non-invasive CWD detection in asymptomatic deer and deer habitats, and enable conceptual advances in our understanding of the disease.

Towards a self-regulation model of career competencies: A systematic review and future research agenda

Owing to the growing emphasis on self-managed career patterns, career competencies as essential personal career resources play a vital role in several work and career outcomes. Despite extensive research on career competencies in the last three decades, it lacks a consistent theorization and often relies on diverse theoretical perspectives. To synthesize our scholarly knowledge of career competencies, we conducted a systematic literature review of 80 peer-reviewed articles from 1985 to November 2022. In doing so, we understand how the construct of career competencies has been conceptualized and measured, review prominent theoretical perspectives adopted, and build a theoretical model based on a self-regulation perspective. This review helped us identify significant research limitations and locate promising research gaps. Building on these insights, we craft a future research agenda highlighting opportunities for conceptual, theoretical, and empirical advancement of career competencies research.

Networked lives: Probing the influence of social networks on the life course

Social network research is well-equipped to help life course scholars produce a deeper and more nuanced approach to the principle of “linked lives,” one of the cornerstones of the field. In this issue on Networked Lives, nine original articles and two commentaries generate new theories, empirical findings and methodological applications at the intersection of the fields of social networks and life course research. In this introduction, we reflect on these advances, highlighting key findings and challenges that await scholars in building more robust synergy between the two fields. Social networks emerge as key structural forces in life courses, yet there is much to learn about the mechanisms through which their effects on people’s lives come about. There is a need to study further how networks evolve through the rhythm of life events, and to analyze broader and more complex networks that capture the roles and influences of relations beyond intimate or family ties. These papers demonstrate that there is much to be gained in probing how individuals are linked to and unlinked from others over time, and in carrying conceptual and methodological advances across social network and life course studies.

Roles and regulation of tRNA-derived small RNAs in animals.

A growing class of small RNAs, known as tRNA-derived RNAs (tdRs), tRNA-derived small RNAs or tRNA-derived fragments, have long been considered mere intermediates of tRNA degradation. These small RNAs have recently been implicated in an evolutionarily conserved repertoire of biological processes. In this Review, we discuss the biogenesis and molecular functions of tdRs in mammals, including tdR-mediated gene regulation in cell metabolism, immune responses, transgenerational inheritance, development and cancer. We also discuss the accumulation of tRNA-derived stress-induced RNAs as a distinct adaptive cellular response to pathophysiological conditions. Furthermore, we highlight new conceptual advances linking RNA modifications with tdR activities and discuss challenges in studying tdR biology in health and disease.

Small-Angle X-Ray Scattering for Macromolecular Complexes.

Small angle X-ray scattering (SAXS) is a versatile technique that can provide unique insights in the solution structure of macromolecules and their complexes, covering the size range from small peptides to complete viral assemblies. Technological and conceptual advances in the last two decades have tremendously improved the accessibility of the technique and transformed it into an indispensable tool for structural biology. In this chapter we introduce and discuss several approaches to collecting SAXS data on macromolecular complexes, including several approaches to online chromatography. We include practical advice on experimental design and point out common pitfalls of the technique.

DIALECTICS OF INTERNAL COLONIZATION

The postcolonial paradigm has evolved through various conceptual advancements, from the anthropological concept of “skin” as the basis of dual oppression based on class and race to the semiotic treatment of “skin” as a social interface constructed in practices of “writing on bodies” and discourses. The decolonization in our region raises a new set of problems. On the one hand, it refers to a complex cultural palimpsest. As a basic text of modernity, we have the colonization of the countryside by the city. But on top of the division of the city and the countryside other dividing lines are superimposed: ethnolinguistic divisions (Russian, Ukrainian, Belarusian) and the divisions of imperial and democratic forces. On the other hand, the figure of the Russian colonizer has a specific, neurotic character — a “neurotic view” of its position in the world, the fear of being colonized. And this neurotic fear is displaced from external enemies to internal ones. The Russian Empire, confronting external forces, constantly encounters not only an imaginary enemy, but imperceptibly turns back to itself as a colonized colonizer.

Specificities of orofacial neuropathic pain

Head pain and notably orofacial pain differs from spinal pain on pathophysiological, clinical, therapeutic and prognostic levels. Its high prevalence, important impact on quality of life and significant socio-economical burden justify specific study of such type of pain. Among them, neuropathic orofacial pain resulting from disease or trauma of the trigeminal nervous system is among the most difficult types of pain to diagnose and to treat. Deciphering of underlying peripheral and central mechanisms has allowed numerous conceptual, clinical and therapeutic advances, notably the role of neural and non neural cell types, such as glia, immunocytes, vascular endothelial cells or the role of trigeminal sensory complex neural circuitry reconfiguration in the development of post-traumatic trigeminal neuropathic pain. Cellular interactions within the trigeminal ganglion, allowing a better understanding of several painful dental, ocular or cephalalgic comorbidities, are also described.

Fabrication of innovative multifunctional dye using MXene nanosheets.

The increasing demand for natural and safer alternatives to traditional hair dyes has led to the investigation of nanomaterials as potential candidates for hair coloring applications. MXene nanosheets have emerged as a promising alternative in this context due to their unique optical and electronic properties. In this study, we aimed to evaluate the potential of Ti3C2Tx (Tx = -O, -OH, -F, etc.) MXene nanosheets as a hair dye. MXene nanosheet-based dyes have been demonstrated to exhibit not only coloring capabilities but also additional properties such as antistatic properties, heat dissipation, and electromagnetic wave shielding. Additionally, surface modification of MXene using collagen reduces the surface roughness of hair and upregulates keratinocyte markers KRT5 and KRT14, demonstrating the potential for tuning its physicochemical and biological properties. This conceptual advancement highlights the potential of MXene nanosheets to go beyond simple cosmetic improvements and provide improved comfort and safety by preventing the presence of hazardous ingredients and solvents while providing versatility.

DNA Barcoding in Species Delimitation: From Genetic Distances to Integrative Taxonomy.

Over the past two decades, DNA barcoding has become the most popular exploration approach in molecular taxonomy, whether for identification, discovery, delimitation, or description of species. The present contribution focuses on the utility of DNA barcoding for taxonomic research activities related to species delimitation, emphasizing the following aspects:(1) To what extent DNA barcoding can be a valuable ally for fundamental taxonomic research, (2) its methodological and theoretical limitations, (3) the conceptual background and practical use of pairwise distances between DNA barcode sequences in taxonomy, and (4) the different ways in which DNA barcoding can be combined with complementary means of investigation within a broader integrative framework. In this chapter, we recall and discuss the key conceptual advances that have led to the so-called renaissance of taxonomy, elaboratea detailed glossary for the terms specific to this discipline (see Glossary in Chap. 35 ), and propose a newly designed step-by-step species delimitation protocol starting from DNA barcode data that includes stepsfrom the preliminary elaboration of an optimal sampling strategy to the final decision-making process which potentially leads to nomenclatural changes.

Are nursery approaches maturing in their application to US fisheries management?

Abstract There have been significant conceptual advances for identifying nursery habitats within coastal systems used by juvenile fishes and crustaceans. The approaches for delineating nursery areas include measures of juvenile abundance, growth, or survival; habitat characteristics; seascape connectivity; population fitness; and contribution to adult biomass (per unit area or total). We used all US coastal states as replicate trials to evaluate the integration of nursery concepts into fisheries management and found an obvious disconnect between expanding academic interest in nursery roles and management application. Among the few states that afford a subset of coastal environments with nursery status, easily obtained metrics (e.g., juvenile density or presence/absence of biogenic structure) are used, and it remains unclear what role nursery designations have played in promoting sustainable fisheries. Gathering the data necessary to use higher-order nursery approaches and metrics (e.g., connectivity and biomass contribution) to designate nurseries will require significant research investment and greater collaboration between ecologists and fisheries scientists.