Traditional Chinese Medicine Concepts Research Articles

Understanding Traditional Chinese Medicine to strengthen conservation outcomes

AbstractNumerous treatments in Traditional Chinese Medicine (TCM) involve the use of wildlife products, including some that utilize ingredients derived from endangered flora and fauna. Demand for such endangered wildlife products in TCM can threaten the survival of species and pose serious challenges for conservation.Chinese medical practice is embedded in the cultural fabric of many societies in East and Southeast Asia, and remains an integral part of everyday life and knowledge. It is grounded in principles and theories that have grown over hundreds of years and differ substantially from those of mainstream allopathic biomedicine.In order to address the threats posed by the medicinal consumption of endangered wildlife, conservation scientists and practitioners will benefit from a basic understanding of TCM. Such knowledge will enable conservationists to craft culturally nuanced solutions and to engage constructively with TCM stakeholders. However, conservationists typically lack familiarity with TCM as the incompatibility of many TCM concepts with those of the biomedical sciences poses a barrier to understanding.In this paper, we examine the core theories and practices of TCM in order to make TCM more accessible to conservation scientists and practitioners. A better understanding of TCM will enable conservationists to deliver more effective and lasting conservation outcomes.A freePlain Language Summarycan be found within the Supporting Information of this article.

Acupuncture in Temporomandibular Disorders Painful Symptomatology: An Evidence-Based Case Report.

Temporomandibular dysfunction (TMD) is complex and multifactorial. Its etiology involves various factors, such as malocclusion, psychological patterns, parafunctional habits, and the temporomandibular joint anatomy. Symptoms include myofascial pain, joint clicking, mouth opening limitation, headaches, earaches, and neck pain, comprising one of the greatest causes of nondental pain. Acupuncture has been used to treat these conditions by acting locally both in muscle relaxation and pain management as well as reaching physical, mental, and emotional balance. The aim of this study is to present a brief literature review reporting the efficacy of acupuncture on TMDs and a case report in which the painful symptomatology of TMD was treated with a systemic protocol, based on scientific evidence and concepts of traditional Chinese medicine. Acupuncture may be a complementary treatment for TMD, and in this case report, it eliminated the patient’s painful symptomatology.

Preliminary observation of the migration of sodium fluorescein along meridians in the limbs of mini-pigs

<p indent=0mm>Meridians are a core concept in traditional Chinese medicine (TCM) theory. It has been shown in many previous studies that meridians exist in the interstitium, the space through which interstitial fluids flow. Sodium fluorescein (SF) is a small, fluorescent, hydrophilic molecule. SF was injected into the low impedance points (LIPs) or the high impedance points (HIPs) on the limbs of healthy mini-pigs, as determined by a low impedance meridian locator (WQ6F30). Migration tracks were scanned by a digital camera (Canon 5D2) with a narrow band filter and colored flash lamps prior to and after SF injection. The relationships between superficial veins and SF tracks were analyzed using a projection vein finder (V800P). Tissues were frozen on dry ice, and the distribution of SF in the transverse sections of SF tracks was observed. Results reliably showed that seven migrating tracks along the low impedance line along meridians (LILM) were found when SF was injected at LIPs, three of which were on the outside of forelimb, three of which were on the inside of forelimb, and one of which was on the inside of hind limb. The average length of these migration tracks was <sc>5.13 cm.</sc> The migration tracks were 81.4% consistent with LILM, while no overlap was observed with veins. The shape of injected SF in the transverse sections was similar to an inverted triangle, and distributed in the muscle gap. No linear migration was observed when SF was injected at HIP, and the shapes of injected SF were nearly round by local diffusion. The length, width and length-width ratio of SF shapes between injections at LIPs and HIPs were significantly different (<italic>P</italic><0.01). These results imply that the concept of the meridian in TCM may be an unknown interstitial channel.

Treating Different Diseases With the Same Method-A Traditional Chinese Medicine Concept Analyzed for Its Biological Basis.

IntroductionThe fundamental theory of traditional Chinese medicine (TCM) implies that when different diseases have the same pathogen, the syndromes of these individual diseases will be the same. “Treating different diseases with the same method” is a TCM principle suggesting that when different diseases have similar pathological changes during different stages of their development, the same method of treatment can be applied. Our study aims to analyze the concept “treating different diseases with the same method” from a molecular perspective, in order to clarify its biological basis and to objectively standardize future TCM syndrome research.ObjectiveThe TCM syndromes Qi deficiency and blood stasis have similar pathogenesis in relation to coronary heart disease (CHD) and stroke. We aim to use big data technology and complex network theory to mine the genes specifically relevant to these TCM syndromes. This study aims to explore the correlation between the biological indicators of CHD and stroke from a scientific perspective.MethodsMining the relevant neuroendocrine-immune (NEI) genes by means of gene entity recognition, complex network construction, network integration, and decomposition to categorize relevant syndrome terms and establish a digital dictionary of gene specifically related to individual diseases. We analyzed the biological basis of “treating different diseases with the same method” from a molecular level using the TCMIP v2.0 platform in order to categorize the TCM syndromes most relevant to CHD and stroke.ResultsWe found 46 genes were involved in the TCM syndromes of Qi deficiency and blood stasis of CHD and stroke. The same genes and their molecular mechanism also appeared to be in close relation to inflammatory response, apoptosis, and proliferation.ConclusionBy using information extraction and complex network technology, we discovered the biological indicators of the TCM syndromes Qi deficiency and blood stasis of CHD and stroke. In the era of big data, our results can provide a new method for the researchers of TCM syndrome differentiation, as well as an effective and specific methodology for standardization of TCM.

Development and validation of a diagnostic risk score for assessing a TCM condition, Protective Qi Deficiency, in adults

IntroductionStandardized diagnosis is required and therefore advocated for evidence-based research in Traditional Chinese Medicine (TCM). In this study, we aimed to standardize and validate a protocol that assesses Protective Qi Deficiency (PQD), a common TCM condition that predisposes individuals to pathogenic invasion. Methods3 TCM practitioners evaluated 151 participants for PQD status via conventional TCM methods and a standardized protocol consisting of 13 PQD-related variables identified as potential predictors of a diagnostic algorithm of PQD. The collected data served as the training dataset to determine which predictors that significantly contributed to PQD diagnosis and their coefficients in the resulting algorithm. The algorithm was then reviewed and modified by an independent expert panel following the Delphi method, and subsequently validated using a separate validation dataset (n = 56). ResultsThe Delphi-modified algorithm contained 8 predictor variables and accounted for 73.4 % of the PQD variance in the training dataset. Using the validation dataset, the algorithm predicted a TCM practitioner’s conventional diagnosis of PQD with a high degree of accuracy. The protocol also demonstrated significant precision as demonstrated by a high between-practitioner consistency (0.993) measured by intraclass correlation coefficient. ConclusionOur study provides evidence that, through rigorous standardization, quantitation, and statistical analysis, reliable assessment of a TCM concept can be achieved and applied for researching TCM therapeutics. Nonetheless, the generalizability of these findings need to be tested in future studies with increased numbers of practitioners and participants and in studies conducted at multiple geographical venues.

Thoughts on the naming of “COVID-19”

Based on the naming of diseases in the history and the nomenclature of diseases, especially that of novel human infectious diseases, in traditional Chinese medicine (TCM) and modern medicine, we put forward the following suggestions for the naming and severity classification of “coronavirus disease 2019 (COVID-19)”: (1) Patients with only nucleic acids positive or nucleic acid positive as well as some of the symptoms but without any evidence of pneumonia should be diagnosed more generally such as “Novel coronavirus respiratory infection (NCRI)” or “Novel coronavirus infection (NCI)”. (2) The manifestations concerning pneumonia can be used as the main basis for the classification of the severity of the disease. For instance, those with only nucleic acids positive or nucleic acid positive as well as mild symptoms are mild, those with nucleic acid positive as well as symptoms like fever and cough are moderate, those with nucleic acid positive as well as pneumonia are severe, those with severe symptoms as well as respiratory failure and multiple organ damage are critical. (3) Also, those with infections and clinical manifestations but no pneumonia can be called simple type, and those with pneumonia can be called pneumonia type. (4) Under the current background of integrative medicine, the naming of newly emerging infectious diseases by TCM should be changed from an ambiguous concept to a clearly defined one. It may define the disease according to the etiology, pathogenesis, clinical manifestation or prognosis of the disease, redefine the original concept in TCM and discard the excessively broad part, or carry out the common naming between TCM and modern medicine based on the specific pathogen. (5) According to the nomenclature of diseases in TCM plus that in modern medicine, the NCI may be named “Jihai (2019) — Pestilence” (Ji Hài (2019) — Yì Lì) in TCM.

Quality evaluation of angelica broken wall powder based on QbD concept of traditional Chinese medicine

In this paper, angelica broken wall powder(ABWP) was taken as the research object, HPLC fingerprint combined with multi-component determination(ferulic acid, senkyunolide I, coniferyl ferulate, ligustilide and 3-butylidenephthalide), physical fingerprint(D_(90), particle size distribution range, particle size distribution width, bulk density, tap density, inter-particle porosity, Carr index, specific surface area, pore volume, angle of repose, Hausner ratio, loss on drying and hygroscopicity)were used to characterize the quality attribute of ABWP; similarity analysis, cluster analysis, principal component analysis and orthogonal partial least squares discriminant analysis were conducted to construct the quality evaluation method of holographic analysis based on traditional Chinese medicine QbD &quot;4 H mode&quot;, in order to evaluate the quality of ABWP from different sources and find out differentiated indicators. The quality evaluation method could be used for scientific, comprehensive evaluation of the quality attribute of ABWP, and the quality consistency evaluation of cell-wall-broken powder of different sources or different processes.It provides new ideas for quality control and research of ultrafine granular powders of traditional Chinese medicine.

Game consumption and the 2019 novel coronavirus

Game consumption and the 2019 novel coronavirus

Prevention and Control of COVID-19 in Hemodialysis Centers of Traditional Chinese Medical Institutions

At the beginning of 2020,many areas in China have been affected by Corona Virus Disease 2019(COVID-19),which is strong infectivity.Because of the mobility of the personnel in the blood purification centers,the dialysis patients with low resistance and the particularity of the treatment environment where the patients congregate,it has become a medical place with a high risk of exposure to COVID-19.As the hemodialysis center of traditional Chinese medical institutions,we suggest prevention and control as following:(1)Scientific prevention and control in legal:regulate strictly,strengthen the awareness of prevention and control,scientifically formulate prevention and control plans,and cut off the communication channels.(2)Establish prevention and control methods based on correct knowledge of traditional Chinese medicine(TCM):In TCM,the COVID-19 is a category of"damp toxin epidemic",which is located in the lung.The basic pathogenesis is characterized by"wet,toxic,stasis and closed",which emphasizes syndrome differentiation and treatment.(3)Formulate prevention and control measures based on the concept of TCM:The key to prevent and control the COVID-19 is to maintain healthy qi and avoid epidemic diseases.(4)Adhere to the integration of Chinese and Western medicine,and build a prevention and control system with characteristics of TCM:TCM has many advantages in the prevention and treatment of infectious diseases,the hemodialysis centers should adhere to the integration of traditional Chinese and Western medicine,and build the prevention and control system of infectious diseases with characteristics of TCM.

Topological Analysis of the Language Networks of Ancient Traditional Chinese Medicine Books.

This study aims to explore the topological regularities of the character network of ancient traditional Chinese medicine (TCM) book. We applied the 2-gram model to construct language networks from ancient TCM books. Each text of the book was separated into sentences and a TCM book was generated as a directed network, in which nodes represent Chinese characters and links represent the sequential associations between Chinese characters in the sentences (the occurrence of identical sequential associations is considered as the weight of this link). We first calculated node degrees, average path lengths, and clustering coefficients of the book networks and explored the basic topological correlations between them. Then, we compared the similarity of network nodes to assess the specificity of TCM concepts in the network. In order to explore the relationship between TCM concepts, we screened TCM concepts and clustered them. Finally, we selected the binary groups whose weights are greater than 10 in Inner Canon of Huangdi (ICH, 黄帝内经) and Treatise on Cold Pathogenic Disease (TCPD, 伤寒论), hoping to find the core differences of these two ancient TCM books through them. We found that the degree distributions of ancient TCM book networks are consistent with power law distribution. Moreover, the average path lengths of book networks are much smaller than random networks of the same scale; clustering coefficients are higher, which means that ancient book networks have small-world patterns. In addition, the similar TCM concepts are displayed and linked closely, according to the results of cosine similarity comparison and clustering. Furthermore, the core words of Inner Canon of Huangdi and Treatise on Cold Pathogenic Diseases have essential differences, which might indicate the significant differences of language and conceptual patterns between theoretical and clinical books. This study adopts language network approach to investigate the basic conceptual characteristics of ancient TCM book networks, which proposes a useful method to identify the underlying conceptual meanings of particular concepts conceived in TCM theories and clinical operations.

Analogous Systems Principles and Concepts of Traditional Chinese Medicine and Congruence Couple Therapy

Open access article. Creative Commons Attribution-NonCommercial-ShareAlike 4.0 Licence (CC BY-NC-SA 4.0) applies

Tongue colour and coating prediction in traditional Chinese medicine based on visible hyperspectral imaging

Tongue diagnosis is an important concept in Traditional Chinese Medicine (TCM). The tongue colour and coating can aid understanding of the body's physiological mechanisms, as well as the pathology of diseases. Existing research has focused on using digital images and tongue colour classification, without considering the other visible bands of information in the tongue. In this study, a visible hyperspectral image system, with an approximate spectral range of 400–1000 nm, was used to predict the tongue colour values and the coating position in TCM, and a stacked autoencoder (SAE) predict model based on spectral–spatial feature was performed to digital the tongue colour space and the coating. The experimental results show the effectiveness of the spectral–spatial feature with SAE model in predicting the CIELAB values of L, a, and coating position, thus the authors provide a new technique for the objective and digitising development of TCM.

Choe Han-gi's Discourse on Singi and His Criticism on Chinese Medicine and Western Medicine: Focusing on the Relationship with Seo Gyeong-deok's Philosophy.

This study examines how Choe Han-gi (1803-1879) developed his medical discourse which integrated the concepts of traditional Chinese medicine with modern Western anatomy, based on the philosophy of Seo Gyeong-deok (1489-1546), a scholar of the Neo-Confucianism of Joseon (1392-1910). Seo emphasized gi (C. qi, vital, material force) rather than yi (C. li, the principle of things) as a way of understanding the world. Since Choe's early academic interests pertained to Neo-Confucianism, it is reasonable to examine his philosophy in this context. Similar to Seo, Choe assumed that the most essential component of the world was the intrinsic and mysterious gi. Although Seo spoke of gi as a damil cheongheo ji gi (the gi which is profound, uniform, clear, invisible, and empty), Choe preferred to use the word singi (C. shenqi, the intrinsic, invisible, and mysterious gi). He believed that the earth, moon, and stars operated through the action of singi and that all creatures could only exist by relying on it. Singi was the most important premise in Choe's medical discourse, a fact demonstrating that although he could be very critical of traditional Chinese medicine, his perspective was part of that tradition. He believed that singi integrated and operated the entire human body and that it perceived external objects. He also emphasized the role of hyeongjil (C. xingzhi, a visible object with a form and quality; here it means all human bodies). This was the medium through which singi could appear in reality. Choe thought that singi could not reveal itself in reality without hyeongjil, and that hyeongjil became a dead thing without singi. His perception of the role of hyeongjil was expressed in his interest in modern Western anatomy, an interest that complemented his focus on singi. In light of his understanding of the singi-hyeongjil relationship, Choe criticized both modern Western anatomy and traditional Chinese medicine. He thought that modern Western anatomy lacked awareness of singi and that traditional Chinese medicine lacked accurate knowledge of human anatomy. Although he was not completely sympathetic toward any forms of medicine, he was open to ideas from both Western and Chinese medicine. Choe could not accept Western anatomy as fully as Japanese intellectuals did. The study of anatomy in Japan had developed in relation to the idea of Ancient Learning (C. guxue), which denied such theories of systematic correspondence as Yin and Yang and the Five Elements (C. yinyang wuxing) and tended to focus on the action of hyeongjil itself. Because Choe accepted modern Western anatomy without accepting Ancient Learning, his perspective was unique in the history of East Asian anatomy. From a medical history perspective, how does Choi Han-gi's medical discourse distinguish itself from other medical discourses, and what are its characteristics? In addition to other explanations, focusing on the political imagination associated with medicine can help illuminate the differences between the medical discourse of Choe and those of others. Discussion of medicine and the human body was tied to political thought, manifesting the political imagination of the society in which that discussion took place. The development of Western and Japanese anatomy reflected a vertical and hierarchical political order, exemplified by the belief that the brain was the center of the body. However, Choe doubted that organs like the brain or heart dominated the body. In his view, the singi ruled the body; it was not a specific organ, and it was equally inherent in all people. His political thought also emphasized the horizontal and equal order among people. His view of singi simultaneously influenced both his perspective on medicine and his perspective on society. Choe Han-gi's belief in this horizontal and equal political order was inherent in his singi-centered medical discourse.

Microbial and metabolomic remodeling by a formula of Sichuan dark tea improves hyperlipidemia in apoE-deficient mice.

Medicine-food homology is a long-standing concept in traditional Chinese medicine. YiNianKangBao (YNKB) tea is a medicine-food formulation based on Sichuan dark tea (Ya’an Tibetan tea), which is traditionally used for its lipid-lowering properties. In this study, we evaluated the effects of YNKB on dyslipidemia and investigated the mechanism underlying its correlation with gut microbiota and serum metabolite regulation. Wild-type mice were fed a normal diet as a control. Male ApoE-/- mice were randomly divided into three high-fat diet (HFD) groups, a model group, and two treated groups (100, 400 mg/kg/d for low, high-dose), and fed by gavage for 12 weeks. Serum lipid levels, composition of gut microbiota, and serum metabolites were then analyzed before treatment with YNKB. We extracted the ingredients of YNKB in boiled water for one hour. YNKB supplementation at a high dose of 400 mg/kg/day reduced bodyweight gains (relative epididymal fat pad and liver weight), and markedly attenuated serum lipid profiles and atherosclerosis index, with no significant differences present between the low-dose treatment and HFD groups. Gut microbiota and serum metabolic analysis indicated that significant differences were observed between normal, HFD, and YNKB treatment groups. These differences in gut microbiota exhibited strong correlations with dyslipidemia-related indexes and serum metabolite levels. Oral administration of high-dose YNKB also showed significant lipid-lowering activity against hyperlipidemia in apoE-deficient mice, which might be associated with composition alterations of the gut microbiota and changes in serum metabolite abundances. These findings highlight that YNKB as a medicine-food formulation derived from Sichuan dark tea could prevent dyslipidemia and improve the understanding of its mechanisms and the pharmacological rationale for preventive use.

An investigation into the effects of acupuncture on radial pressure pulse waves in patients with low back pain: A protocol for a quasi-experimental study

IntroductionThe characteristics of radial pressure pulse waves (RPPW) provide an essential diagnostic technique in Traditional Chinese Medicine (TCM). The purpose of this research is to develop a study protocol that integrates the concept of TCM and traditional acupuncture treatment with modern scientific tools utilizing a quasi-experimental design. We will investigate the effects of acupuncture on the RPPW in study participants with low back pain (LBP) using modern tools, including the pulse sphygmograph, the fingertip-to-floor test, the Faces Pain Scale-Revised, the Oswestry Disability Index, the Health Status Questionnaire, and the Constitution in Chinese Medicine Questionnaire. MethodsWe will attempt to recruit 80 eligible subjects with LBP based on our predefined inclusion and exclusion criteria. Acupuncture intervention will be performed bilaterally on Shenshu (BL23), Dachangshu (BL25) and Weizhong (BL40) for 20 min. Objective and subjective baseline assessments and outcome evaluations will be performed at a specific time before and after the intervention. This paper describes the methods of our original research approved by the China Medical University Hospital's Research Ethics Committee. Recruitment is in progress and data collection will continue until March 2019. ConclusionsTo our knowledge, this preliminary study is the first attempt to investigate the effects of acupuncture on the RPPW in LBP subjects using a pulse sphygmograph and other modern tools. The findings will also investigate the effectiveness of the selected acupuncture point combinations for LBP. We hope this preliminary study will provide a basic foundation for a large-scale research study that involves randomisation in the future.

Traditional Chinese Medicine Explanatory Models of Depressive Disorders: A Qualitative Study

Traditional Chinese medicine (TCM) is an alternative medical system utilised by many Chinese. However, the knowledge of TCM concepts of depression is limited amongst clinicians with training in Western biomedicine. The purpose of this study was to obtain a better understanding of the conceptualisation of depression from a group of TCM practitioners. Semi-structured interviews in Chinese were carried out with 10 TCM practitioners in Hong Kong. A case description of major depression disorder (MDD) was used as a basis. Interview texts were transcribed, translated and analysed using qualitative content analysis. Most informants identified the case as a depression pattern, a term that lacked clear definition and standardised criteria. The mechanism of disease for MDD symptoms were regarded to be liver-qi dysregulation and an imbalance of yin and yang. The TCM practitioners implemented individualised diagnosis, treatment, and a holistic concept without clear distinction between the mind and the body. This contrasted with the biomedical tradition of separating psychologisation and somatisation. The meanings given to the concept of depression did not correspond with current DSM or ICD definitions, and the TCM normativity can result in variations in explanatory models.

Acupuncture in Obstetrics and Gynecology

Importance Acupuncture is a key component of therapy in traditional Chinese medicine. Only in the last few decades has acupuncture become popular in the United States. The mechanism behind acupuncture's effects on the body and brain has not been completely elucidated, but there is evidence that acupuncture has effects on the endocrine, immune, and sympathetic and parasympathetic nervous systems. It may also act through electrical and mechanical signaling through the connective tissue and fascia. Despite this uncertainty, the positive effects of acupuncture have been well established. Objective In this review, we discuss the basic concepts of traditional Chinese medicine and acupuncture and examine the evidence regarding the use of acupuncture in obstetrics and gynecology. Evidence Acquisition We conducted a literature review of acupuncture in obstetrics and gynecology using PubMed. Results The available data demonstrate that acupuncture is beneficial in the treatment of labor pain, back pain in pregnancy, and dysmenorrhea. It is unclear if acupuncture is effective for hyperemesis, chemotherapy-induced nausea and vomiting, and menopausal hot flushes, or if moxibustion is effective in correcting breech presentation. There are limited but positive data regarding menopause-related sleep disturbances, depression in pregnancy, and overactive bladder. Acupuncture is not beneficial for improving outcomes in in vitro fertilization. Conclusions and Relevance Acupuncture is an increasingly popular therapy with many potential applications in obstetrics and gynecology. A general understanding of the basic principles of acupuncture and the safety and efficacy of its practice is necessary for the general obstetrician and gynecologist to make informed recommendations to patients. Target Audience Obstetricians and gynecologists, family physicians. Learning Objectives After completing this activity, the learner should be better able to identify the basic concepts of traditional Chinese medicine and how it differs from Western medicine; describe common obstetrical and gynecologic issues that can be successfully treated with acupuncture; and distinguish the main issues in designing acupuncture studies regarding blinding, choosing a control group, and identifying areas of potential bias.

"Pregnancy Brain": A Review of Cognitive Changes in Pregnancy and Postpartum.

The idea that pregnant women suffer from deficits in memory is widespread but poorly documented in obstetrical literature. To review available psychology literature on the subject of cognitive changes in pregnancy in order to guide the prenatal care provider on how to counsel pregnant women. An extensive review of PubMed and PsycINFO databases was conducted to gather and analyze relevant studies on cognitive changes in pregnancy. A large body of literature examining basic science and animal studies support the effects of pregnancy hormones in remodeling brain architecture and neural function. However, studies in humans are hampered by small sizes, heterogeneous methodology, and varying definitions of memory. Nevertheless, the consensus among researchers is that pregnancy does confer deficits in working memory relative to nonpregnant controls. Cognitive effects of pregnancy are likely small, and the impact on daily life is debatable. Further research is needed to determine whether these effects may confer an advantage to parous women comparable to that seen in lower mammals.

Base on concept of traditional Chinese medicine: Experimental studies on efficacy of BIOCERAMIC Resonance to alleviate drug withdrawal symptoms

Those who are challenged by dependency on prescription drugs or suffer drug addictions have few options available to them for recovery, such as psychotherapy and physiotherapy. Here we present a new approach with clinical examples involving stimulant addiction or overdose of hypnotic drugs that were received BIOCERAMIC Resonance, which was developed based on concept of 12 meridian channels of traditional Chinese medicine, and has successful withdrawal or dose reduction benefits. We describe the whole process and the clinical outcome. And by help of our previous publication on functional MRI, we discuss the possible brain locations response to BIOCERAMIC Resonance that may be corresponding to the beneficial effects of relief of depression, sleep deprivation and other mental symptoms that associate with substance abuse and withdrawal effects. We suggest this could be potentially widely application on substances abuse.

Genetic and chemical differentiation characterizes top-geoherb and non-top-geoherb areas in the TCM herb rhubarb

Medicinal herbs of high quality and with significant clinical effects have been designated as top-geoherbs in traditional Chinese medicine (TCM). However, the validity of this concept using genetic markers has not been widely tested. In this study, we investigated the genetic variation within the Rheum palmatum complex (rhubarb), an important herbal remedy in TCM, using a phylogeographic (six chloroplast DNA regions, five nuclear DNA regions, and 14 nuclear microsatellite loci) and a chemical approach (anthraquinone content). Genetic and chemical data identified two distinct groups in the 38 analysed populations from the R. palmatum complex which geographically coincide with the traditional top-geoherb and non-top-geoherb areas of rhubarb. Molecular dating suggests that the two groups diverged in the Quaternary c. 2.0 million years ago, a time of repeated climate changes and uplift of the Qinghai-Tibetan Plateau. Our results show that the ancient TCM concept of top-geoherb and non-top-geoherb areas corresponds to genetically and chemically differentiated groups in rhubarb.