Biomimicry studies natural systems and attempts to use the gained knowledge and understanding to solve human problems. Can biomimicry, if applied in urban planning, help to make our cities more sustainable or, precisely, more friendly for walkable and 15-minute city models? Various researchers identify the following features of natural systems as form fits function, catalysis of cooperation, local contextuality, continuity of development, diversity, integrity, redundancy, decentralization, multifunctionality, and less energy consumption (e.g. TOD if the energy needed for transportation is considered), hierarchy, fractality, etc. The presented research has two objectives: analyze the possibility of describing and evaluating the degree of expression of the features of natural systems in urban spatial structures to use them for comparison, benchmarking, or creation of a biomimicry index; evaluate if cities with more strongly expressed biomimicry features could be considered more walkable and close to the 15-minute city concept. To give answers to the above-presented questions Riga, Vilnius, and Tallinn were investigated. They represent both certain similarities and differences in terms of history, urban planning, political context, size, etc. A simulative mathematical graph model was used as the main tool to describe and analyze complex urban structures based on spatial configurations. Various graph centralities were employed to describe and compare the expression of natural systems’ features in the selected cities. OSM data was used to overlap graph models with such information as the density of inhabitants, concentrations of various points of interest in order to evaluate walkability and 15-minute city friendliness. The research results could be seen as an important step for better understanding of informal principles of biomimicry that can help transform urban areas.