Kasahara, K, Konrad, A, Murakami, Y, Thomas, E, and Nakamura, M. Comparison of the effects of a roller massager intervention on the rectus femoris between lengthened and slack positions. J Strength Cond Res XX(X): 000-000, 2024-A roller massager (RM) is a type of foam rolling device, which previous studies have shown to be effective for acutely decreasing tissue hardness and increasing range of motion (ROM). However, these effects may differ with the RM intervention position (i.e., knee flexion or knee extension). Therefore, this study aimed to compare the acute effects of an RM intervention on the rectus femoris (RF) in the knee flexed and extended positions. The subjects were 14 healthy male college students (age, 22.6 ± 0.8 years). The RF of the dominant leg was tested. Three conditions were compared: a control condition with no intervention (CON), an RM intervention in knee extension (i.e., RM_extension), and a RM intervention in knee flexion (i.e., RM_flexion). The measurements were tissue hardness in the proximal (TH_pro), middle (TH_mid), and distal (TH_dis) portions of the RF, knee flexion ROM, pain pressure threshold (PPT), maximal voluntary isometric contraction (MVC-ISO) torque, and maximal voluntary concentric contraction (MVC-CON) torque in the RF before and immediately after the intervention. There were significant interaction effects of TH_mid and knee flexion ROM. Both RM_extension and RM_flexion conditions showed a significant (p < 0.01) decrease in TH_mid and an increase in knee flexion ROM, but there were no significant differences between conditions. There was a main effect for TH_pro and PPT (p < 0.05) but no significant interaction effect or main effect for TH_dis, MVC-ISO torque, and MVC-CON torque. The results showed that the RM intervention can effectively decrease tissue hardness and increase ROM, regardless of limb position (i.e., knee extension or flexion).