PURPOSE: To evaluate changes in myostatin, follistatin and MyoD mRNA gene expression using concentric exercise (CE) and eccentric exercise (EE) as a stimulus to better understand the mechanisms of muscle hypertrophy in young women. METHODS: Single-leg maximal eccentric or concentric isokinetic exercise was performed using the Cybex NormTM dynamometer (Cybex International Inc. Ronkonkoma, New York) at 60° /s. Ten sets of ten repetitions with 20 second rest between sets were completed by each subject. Three muscle biopsies of the vastus lateralis were obtained from the exercised leg at baseline (1st biopsy), 8 hours after the first exercise bout (2nd biopsy), and 8 hours after the seventh and final exercise bout performed 2 weeks after the first exercise session (3rd biopsy). mRNA gene expression was analyzed using RT-PCR methods. 18s ribosomal RNA was used as the internal standard to determine relative expression of mRNAs for myostatin, follistatin, and MyoD. Paired t-tests were used to compare changes in mRNA gene expression within groups (Eccentric or Concentric). Independent t-tests were performed to test group differences (Eccentric versus Concentric). A probability level of P ≤ 0.05 was used to determine statistical significance with Bonferroni adjustments. RESULTS: There were no significant fold changes in the CE group for myostatin, follistatin, and MyoD mRNA gene expression between the first and second biopsies as well as the first and third biopsies (P>0.17). Similarly, there were no significant changes in the EE group for the same variables (P>0.11). In addition, there were no significant differences between the EE and CE groups (P≥0.05). CONCLUSIONS: Our data suggests that a single bout or multiple bouts (7) of maximal eccentric or concentric exercise does not significantly alter myostatin, follistatin, or MyoD mRNA gene expression in young women. Therefore, the type of exercise stimulus does not appear to differentially alter mRNA gene expression of hypertrophic myogenic regulators.