ABSTRACT The concentrations of selected heavy metals including cadmium (Cd), lead (Pb), zinc (Zn), copper (Cu), nickel (Ni) and iron (Fe) in stems and leaves of Artemisia herba-alba medicinal plant and soil samples were evaluated. Results showed that there in heavy metal concentrations of washed and unwashed parts of A. herba-alba from northern site (Irbid), Middle site (Al-Mafraq), and Souhern site (Ma’an) (P < .05). Moreover, the results of this study revealed that there is a correlation between heavy metal levels in medicinal plants and their concentrations in soil. Two standard reference materials of plant (SRM 1790a; spinach, CRM 281; ryegrass) and a standard reference material of soil (GBW 07406) were examined to validate the method used. There were good agreements between the measured values of these standard reference materials and their certified values. In addition to that high recoveries for tested elements were ranged between 91.7–97.7%, 90–96.6%, and 92.2–97.7% in (CRM281, ryegrass), (SRM1570a, spinach) and (GBW 04706, soil) respectively. It is reasonable to conclude that the variation in metal concentration from site to another site as well as from element to another element may be due to the different factors such as traffic volumes, the wind directions, the soil nature, and the element behavior. Based on the results obtained, the health authorities will take in consideration these useful information and direct the residents about the risk of these pollutants.