Total Phosphate Concentrations Research Articles

Morpho-functional groups as an efficient tool for monitoring and management of the Billings reservoir (São Paulo, Brazil)

This research applied the Morphology-Based Functional Groups (MBFGs) combined with classical approaches, such as community descriptor species and phytoplankton classes in the Billings reservoir. This local is the largest freshwater reservoir in the metropolitan region of São Paulo that has around 22 million inhabitants. Two sampling stations differing in predominant uses were studied: Rio Grande, classified as mesotrophic, and Central Body, as super and hypereutrophic. The phytoplankton and limnological variables were collected over six months (n=12). The trophic state index was calculated using phosphorus concentrations. Three canonical correspondence analyses were calculated aiming to examine the effect of environmental variables on the phytoplankton structure from each classification method (descriptor species, classes, and MBFGs), identifying the influence of environmental variables (independent variables) on the abundance patterns of the phytoplankton community (dependent variables). The highest concentrations of chlorophyll a and phytoplankton density were recorded at the site with the highest concentrations of total phosphorous (average of 99.86 μg/L in Central Body). This site was also associated with the highest electrical conductivity, total dissolved solids, turbidity, and concentrations of total nitrogen and chlorophyll a. The main factors associated with phytoplankton distribution were the trophic state and the operational system. It was concluded that the phytoplankton approach through MBFG efficiently responds to fluctuations in the ecological attributes of phytoplankton, and because they are based on morphological features, MBFG reduces the complexity of identifying and classifying organisms at a specific level.

Predicting chlorophyll-a dynamics in the Ashtamudi estuary using ANN and ANFIS techniques

Excessive nutrients and associated high chlorophyll-a concentration are of growing concern to the public health due to the occurrence of eutrophication in aquatic bodies. Continuous monitoring of chlorophyll-a has limitations, thereby necessitating the development of prediction models. Even though chlorophyll-a prediction models are numerous, they rarely fit into estuarine conditions, which is encountered with dynamic mixing of freshwater and sea water. Thus, the present study identifies the most appropriate driving factors that are relevant for estuarine conditions through chlorophyll-a prediction models developed using Artificial Neural Network and Adaptive Neuro Fuzzy Inference System (ANFIS) approaches in the Ashtamudi estuary, India. Salinity, which is an indirect measure of the estuarine mixing intensity, has been used to replicate the dynamic mixing in the estuary. The results indicate that the model incorporating salinity along with total nitrogen, total phosphorous and turbidity well-predicted chlorophyll-a concentration with a coefficient of determination ranging from 0.73 to 0.99. General Regression Neural Network (GRNN) and ANFIS models outperformed Back Propagation Neural Network (BPNN) and Recurrent Neural Network (RNN) models. Of the various ANFIS models, the ones that used Gaussian and Generalized Bell membership functions were most appropriate for the prediction of chlorophyll-a than the models with triangular and trapezoidal membership functions. The study identified that the models that considered salinity were less sensitive and can be used for modelling chlorophyll-a. The chlorophyll-a was observed to have a direct influence of salinity at salinity values greater than 5‰. The study highlighted that estuarine mixing further enhances the primary productivity through increased penetration of light leading to higher chlorophyll-a concentration. Further the study emplasized that the predictive models are important tools fitting well within estuarine environments due to its replicability.

Characterization of the intracellular polyphosphate granules of the phototrophic green sulfur bacterium Chlorobaculum tepidum

The ability to generate polyphosphate (polyP) granules is important for survival for bacteria during resistance to diverse environmental stresses, however the genesis of polyP granules is poorly understood. Chlorobaculum tepidum (Cba tepidum) is a thermophilic green sulfur anoxygenic phototrophic bacterium which uses reduced sulfur compounds as electron donors. The presence of electron rich granules inside the Cba tepidum was reported, but no further information was provided. In this work we used cell thin sections at three different time points of cultivation to observe the biogenesis of the inclusions over time, and the in cell total phosphate concentration was monitored over time as well. Furthermore, the elemental analysis (EDS) of the electron rich inclusions showed the presence of phosphorus and oxygen. The existence of polyphosphate was demonstrated by 31P NMR spectroscopy of cell lysates. Finally, we show that the biogenesis of the phosphorus granules correlates with an abundance of proteins that are closely related to polyphosphate metabolism.

Evaluating impacts of climate and management on reservoir water quality using environmental fluid dynamics code

Climate change and human interference, notably nutrient input, affect the water quality. Nitrogen (N) and phosphorus (P) are pivotal in managing eutrophication. This study investigated the effects of water dynamics and chemical constituents on water quality in Hongfeng Lake, a typical weakly stratified reservoir suffering from algae blooms in Southwest China, using the Environmental Fluid Dynamics Code. Leveraging climate, hydrological, and water quality data, we constructed, calibrated, and validated the temperature-hydrodynamics-water quality-sediment model. Various scenarios were analyzed, including wind speed, air temperature, solar radiation, rainfall, water discharge, N and P external input, and internal release. The findings revealed that no rain and warming increased trophic state index (TSI) and chlorophyll-a (Chl-a) concentration, and no solar radiation initially elevated nitrate concentration, followed by an increase in ammonium concentration. Besides, no solar radiation and changes in rainfall significantly increased total phosphate concentration. The management scenarios of N and P reduction, halving tributary, and mainstream flow scenarios improved water quality and reduced eutrophication. The wind speed under the N and P reduced scenarios showed that a doubling in wind led to increased concentrations of the particulate organic matter, Chl-a, and dissolved oxygen, alongside decreased ammonium and nitrate, while TSI exhibited minimal change. However, 5- and 10-times wind speed scenarios amplified TSI in shallow water, potentially due to a substantial rise in internal nutrient release. The degradation trend observed in drinking water quality amid climate change (warming and flooding) raises concerns regarding health-related risks. These simulations provided the quantified influence of climate change and environmental management strategies on water quality in the weakly stratified reservoir, notably highlighting the looming threat of exacerbated eutrophication due to warming, necessitating more stringent N and P reduction measures compared to current practices.

Climate change effect on the characteristics of raw domestic wastewater, sedimentation pond water, and treated wastewater

The objective of this work was to investigate the effect of climate change on the characteristics of raw domestic wastewater, sedimentation pond water, and treated wastewater. The wastewater samples were collected from Khirbet Assamra wastewater treatment plant (KSWTP) in Azzarga Governorate in the Middle Region of Jordan. Descriptive statistics and inferential statistical tests were used for describtion and comparison purposes. Statistical analysis aimed at evaluating the ability of the sedimentation process to purify the water and assessing the overall wastewater treatment efficiciency of KSWTP. The results of the analysis showed that there are significant differences between the raw domestic wastewater, sedimentation pond water, and treated wastewater in pH (F = 15.486, df = 149, p = .000), the chemical oxygen demand (F = 2486.459, df = 149, p = .000), biochemical oxygen demand (F = 2298.142, df = 149, p = .000), ammonium ion concentration (F = 4645.039, df = 149, p = .000), total Kjeldahl nitrogen concentration (F = 5908.067, df = 149, p = .000), and the concentration of the total suspended solids (F = 715,376, df = 149, p = .000). When the characteristics of the raw domestic wastewater were compared with the characteristics of the treated wastewater, it was found that the wastewater treatment reduced the concentrations of total phosphorous and oil and grease and the counts of faecal coliform bacteria but had no significant effect on the mean water temperature and the concentration of dissolved phosphorous. It can be concluded that domestic wastewater treatment in KSWTP was to a large extent efficient in reducing the concentrations of total phosphorous and oil and grease and the counts of the faecal coliform bacteria in the treated wastewater. Further research is needed to compare levels of performance of other wastewater treatment methods with the method applied in KSWTP

Green solutions for antibiotic pollution: Assessing the phytoremediation potential of aquatic macrophytes in wastewater treatment plants

We compared the ability of one emergent (Sagittaria montevidensis), two floating (Salvinia minima and Lemna gibba), and one heterophyllous species (Myriophyllum aquaticum) to simultaneously remove sulfamethoxazole, sulfadiazine, ciprofloxacin, enrofloxacin, norfloxacin, levofloxacin, oxytetracycline, tetracycline, doxycycline, azithromycin, amoxicillin, and meropenem from wastewater in a mesocosm-scale constructed wetland over 28 days. Antibiotic concentrations in plants and effluent were analyzed using an LC-MS/MS to assess the removal rates and phytoremediation capacities. M. aquaticum did not effectively mitigate contamination due to poor tolerance and survival in effluent conditions. S. minima and L. gibba demonstrated superior efficiency, reducing the antibiotic concentrations to undetectable levels within 14 days, while S. montevidensis achieved this result by day 28. Floating macrophytes emerge as the preferable choice for remediation of antibiotics compared to emergent and heterophyllous species. Antibiotics were detected in plant tissues at concentrations ranging from 0.32 to 29.32 ng g−1 fresh weight, highlighting macrophytes' ability to uptake and accumulate these contaminants. Conversely, non-planted systems exhibited a maximum removal rate of 65%, underscoring the persistence of these molecules in natural environments, even after the entire experimental period. Additionally, macrophytes improved effluent quality regardless of species by reducing total soluble solids and phosphate concentrations and mitigating ecotoxicological effects. This study underscores the potential of using macrophytes in wastewater treatment plants to enhance overall efficiency and prevent environmental contamination by antibiotics, thereby mitigating the harmful impact on biota and antibiotic resistance. Selecting appropriate plant species is crucial for successful phytoremediation in constructed wetlands, and actual implementation is essential to validate their effectiveness and practical applicability.

Nutrient Removal Efficiencies in a Bioretention Cell Using Pre-Treated Coconut By-Product as Carbon Source

Abstract In this research, the appropriateness of alkali pre-treatment on coconut by-products was managed by exposing coconut husk and shell to 2M NaOH. The powdered samples were analysed for morphology observation, Fourier Transform Infrared (FTIR), Particle Size Analyzer (PSA), and water quality tests. Weakening the hemicellulose structure resulted from subjecting the coconut shell to alkali pre-treatment shown in the morphology observation. Furthermore, the FTIR analysis exhibited the presence of O-H stretch in all pre-treated samples representing an indication for occurring the lignin breakdown, while an absence of the C=O functional group was shown in both additive samples after their subjection to alkali pre-treatment. In PSA analysis, the particles of all samples were found finer than the particle size distribution standards, in which the smallest D50 was obtained for the treated coconut shell (TCS), followed by untreated coconut shell (UCS), untreated coconut husk (UCH), and treated coconut husk (TCH). Among all the powdered samples (TCS, UCS, UCH, and TCH) analysed in this study, only TCH values met the ranges provided and recommended by the Urban Stormwater Management Manual for Malaysia (MSMA) and the Australian Department of Sustainability, Environment, Water, Population, and Communities (DSEWPC). Meanwhile, a poor removal rate for total suspended solids (TSS) was attained due to the contribution of TSS by the filter media which caused the TSS rates to surpass the inlet values. Additionally, the presence of a high concentration of total phosphorous (TP) in all the tested samples indicated their capabilities to provide PO43 in the bioretention cell, which is a vital nutrient for the plant’s growth. In contrast, Ammoniacal Nitrogen (AN) with a concentration rate as low as 4 mg/L was observed throughout the test periods which showed a significant reduction compared to the rate of AN at the inlet samples ranged between 5.2 and 11.4 mg/L. The findings indicated that coconut by-products, when subjected to an alkali pre-treatment process, are appropriate for incorporation as additives in bioretention filter media.

Spatial and temporal distribution of nutrients in the Ciliwung River, DKI Jakarta

The Ciliwung River, one of the largest rivers to run through Jakarta, is particularly important for its water quality because it travels through the city’s center and several settlements, highly populated housing areas, and slums. The growth of algae and macrophytes, or aquatic plants, is dependent on the nutrients in the streams. These plants serve as major food sources for fish and other small invertebrates. This study aimed to describe the distribution of nutrients (ammonia, nitrite, nitrate, total nitrogen, and total phosphate) in the Ciliwung River, DKI Jakarta, in terms of both space and time. Water quality was monitored under rainy (February and June 2021) and dry (August and September 2021) conditions. The research area, which included 18 observation stations, was situated in the DKI Jakarta sector along the Ciliwung River. Geographically, all river regions’ concentrations of total nitrogen, nitrate, and total phosphate exceeded the quality criteria; however, ammonia and nitrite tended to be high and did not meet norms across the Ciliwung River area, DKI Jakarta. While ammonia concentrations were greater during the dry season and nitrate concentrations were higher during the rainy season, there was a temporary difference in the concentrations of nitrite, total nitrogen, and total phosphate between the seasons.

The effect of anthropogenic activities on the spatial distribution of total nitrogen and total phosphate in Lake Maninjau

This study aimed to analyse the effect of anthropogenic activities on the spatial distribution of total nitrogen (TN) and total phosphate (TP) in Lake Maninjau, Indonesia, during the dry season. Sampling was carried out at ten observation locations representative for various activities around the lake. Cluster analysis and ANOVA were used to classify pollutant sources and observe differences between TN and TP at each site. Concentrations of TN and TP are categorised as oligotrophic-eutrophic. The ANOVA showed spatially that some sampling locations, such as the Tanjung Sani River, floating net cages, and hydropower areas have different TN concentrations. At the same time, TP levels were consistently significantly different across sampling sites. ANOVA and cluster analysis confirmed that floating net cages were the first cluster and the primary contributor to TN and TP. The second and third clusters come from anthropogenic activities around the lake, such as agriculture, settlement, and livestock. The fourth cluster with the lowest TN and TP is the river that receives the anthropogenic activity load but has a high flow velocity. The cluster change analysis needs to be conducted when there are future changes in the composition of floating net cages, agriculture, and settlements.

Influence of Nutrient Solutions in an NGS® Soilless System on the Yield, Quality and Shelf Life of Fresh-Cut Commercial Mint at Different Harvest Times

The optimal fertilizer concentration for Mentha plants is contingent on the growing systems and harvest time, serving as operational solutions to control and enhance quality and yield. This study aimed to determine the effects of three macronutrients concentration in hydroponic nutrient solution (HNS) during three harvest times on the growth, quality, yield, and shelf life of three mint species (M. spicata L. var. viridis (MV); M. piperita L. (MP); M. spicata L. var. rubra (MR)) grown in a New Growing System (NGS®). Total dry matter (DM), nitrate (NO3−), phosphate (PO43−), and calcium carbonate (CaCO3) concentrations were increased with the addition of higher levels of nutrient fertilization in three species. When the ion concentration of the HNS was increased, total fresh yield decreased. The highest total bacterial count (TBC) was obtained in MR species in the three harvests in all the levels of HNS. The lowest browning potential (BP) and soluble o-quinone (So-Q) levels were observed at second harvest in the MR species with the application of one of the two HNS high in nitrogen (N). In conclusion, the combination of optimal HNS ion concentration and appropriate species is considered essential to obtain suitable yield, quality, and ensure shelf life of mint.

Implications of Anthropic Activities in the Catchment Area of a Temporary Mediterranean Wetland Complex in the South of Spain

The Lagunas de Campillos Natural Reserve and adjacent ponds are fundamentally surrounded by regularly fertilized crop fields and livestock industry, producing leachates which can be found in the ponds. The interest in this Site of European Importance and the RAMSAR wetland complex lies in the habitats within it, which are included in the Directive on Habitats of Community Interest. It is essential to determine the trophic status of the ponds and the quality of these habitats, as well as whether corrective measures need to be established in order to maintain a good environmental status. To characterize and compare the ponds, different parameters were measured, such as conductivity, pH, nutrient concentration, Chl-a concentration, phytoplankton composition, phytoplankton abundance (<20 µm), and the quantification of heterotrophic microorganisms indicating contamination of the aquifers. The obtained results showed that all ponds, except a mesotrophic pond, are eutrophic or even hypertrophic, with high levels of total nitrogen (>8 mg L−1), total phosphorous (>165 μg L−1), and chlorophyll-a concentration. These findings explain the high densities of phytoplankton observed, with the predominant presence of small cells (<3.6 μm ESD). In addition, concentrations of heterotrophs and coliforms are, in some ponds, higher than expected. Eutrophication hinders ecological functions and ecosystem services, which finally affects biodiversity and human wellbeing. Five of the six analyzed ponds are within various protection figures for their essential importance to local and migrating avifauna. Therefore, ponds’ status analysis and the implementation of measures for maintaining ecosystem services and trophic state are fundamental for the sustainable management of the studied area.

Land cover, stream discharge, and wastewater effluent impacts on baseflow sediment and nutrient concentrations in SW Ohio streams

AbstractElevated nutrient and suspended sediment concentrations often result in negative environmental impacts within freshwater environments. Studies that directly compare suspended sediment and bioavailable nutrients between predominantly agricultural and predominantly urban watersheds during baseflow conditions are largely lacking. The purpose of this study was to determine the impacts of land cover, stream discharge, and wastewater treatment plant (WWTP) discharge on nutrient and sediment concentrations, across a large land cover gradient in Southwest Ohio streams. Weekly baseflow samples were collected from eight streams over 1 year from November, 2016 through November, 2017. Total suspended sediment, nitrate, and phosphate concentrations were measured. Results indicate that agricultural land cover and WWTPs increase nitrate and phosphate concentrations in the study area. Total suspended sediment and nitrate concentrations increased with discharge, and discharge was a relatively weak predictor of phosphate concentrations. Seasonal water quality trends varied by parameter and land use also had unique impacts on seasonal water quality trends. Results suggest that to improve water quality in the study area, efforts should focus on improving WWTP effluent treatment and agricultural land management.

Direct Membrane Filtration of Wastewater: A Comparison between Real and Synthetic Wastewater

In this study, a lab scale direct membrane filtration (DMF) system using ultrafiltration membranes was operated to compare synthetic and real wastewater to evaluate their membrane fouling propensity and the partitioning of organics and nutrients during concentration. For fouling prevention, cyclic operation was used which consisted of 90 s of filtration followed by 15 s of relaxation and backwashing conducted every 15 min. The system was tested at a high initial flux of 80 LMH (L/m2·h), and the trials were run until a 90% volume reduction was achieved for each batch. Both the synthetic and real wastewater showed similar fouling propensities and organic and nutrient partitioning. The synthetic and real wastewater had an average flux of 46.3 LMH and 28.5 LMH and an average total chemical oxygen demand rejection of 90.3% and 83.1% after 30 h of operation, respectively. The recovery of organics was similar in both influents, resulting in 65.5% and 64.0% of the total chemical oxygen demand concentrations in the concentrate stream for synthetic and real wastewaters, respectively. The total phosphorous and nitrogen concentrations were also similar in terms of rejection rates resulting in 85% and 78% for the synthetic and 89% and 65% for the fresh WWs, respectively. The comparison revealed that synthetic wastewater, though not identical to real wastewater, can serve as a surrogate in DMF studies. This will help to remove one of the key sources of variability in current DMF studies and will allow for more rapid development of DMF technology.

Evaluating total nitrogen and phosphorous concentrations in a watershed impacted by diverse anthropic activities in a developing country.

This research aims to identify critical contamination points by nutrients, their possible origin (point and nonpoint sources), their spatial distribution, and possible attenuation by natural and anthropogenic processes. The study area is the Velhas River Basin, located in the Southeast Region of Brazil (17.0°-20.5° S; 43.5°-45.0°W). A historical series of water quality monitoring, land cover map, demographic and agricultural censuses, sewage treatment diagnostics, and local hydrographic networks were used to achieve the objectives. In addition, the regions were divided into incremental areas, enabling individualized analyses of each sub-basin. Descriptive statistics, seasonality, categorized data tests, agglomerative hierarchical cluster analysis, and principal component analysis were used. There was a significant contribution of nutrients in the most important urban agglomeration of the basin, resulting in peak concentrations measured at that place. Although the values were reduced by the mouth (650 km), the percentage of legislation violations remained high. The effects of punctual contamination were intensified by the low percentage of treated sewage in the basin, the absence of adequate treatment technologies to remove nutrients, and the disorderly urbanization. Furthermore, it was estimated that the nutrient load from animal husbandry is approximately 75% of the load from domestic effluents due to the high number of cattle in the basin and the low percentage of forests.

Modelling of sanitary wastewater composition and operation of a small membrane bioreactor wastewater treatment plant with denitrification and nitrification

The ratio of concentrations of total nitrogen and five-day biochemical oxygen demand in the sanitary wastewater of an energy infrastructure facility in Serbia is many times higher than usual, resulting in only half of the total nitrogen being eliminated in wastewater treatment plants (membrane bioreactor with anoxic and aerobic reactors) by denitrification. The first step of analysis was mathematical modelling of the composition and origins of the input wastewater. The model was developed based on the scientific literature data on composition of human excrement and the data on composition of water used for sanitary purposes. Next, it was successfully verified by comparisons to the experimental data of the wastewater composition. In the next step, a model for wastewater treatment simulation was created by using the BioWin software (Envirosim Associates, USA) in order to examine functioning of the plant and test the effects of several possible modifications of the process. A good agreement with the qualities of the influent and effluent determined by laboratory analyses was achieved after model calibration. The results of simulations showed a tenfold decrease in the total nitrogen concentration and a fortyfold decrease in the total phosphorous concentration in the effluent after introducing the following modifications to the simulated process: ferric chloride dosing, increasing dosing of acetate in the anoxic reactor by a factor of seven, increasing of the waste activated sludge rate by a factor of four and increasing the recirculation flow rate by a factor of three.

Growth, Biomass Production, Pollutant Removal Capacity of Chlorella Vulgaris Cultivated in Water Polluted with Phosphates, Nitrates, Cadmium and Lead Ions; A Possible Strategy for Bioremediation, Carbon Dioxide Utilization and Simultaneous Biodiesel Productivity

The main objective of the work reported herein was to study the possibility of simultaneous use of microalgae for bioremediation and biodiesel production. Determination of optimum nitrate and phosphate concentrations for the maximum algal growth in the presence of heavy metal ions in the growth media was the other objective. In this regard, Chlorellavulgaris, a microalga, was cultivated separately for 30 days at ~28 °C in the presence and absence of known cadmium, lead, phosphate ion concentrations in growth media containing an optimum nitrate ion concentration. The concentrations of these ions in the growth media, dry algal biomass and extractable algal-oil percentage after 30 days of their growth were determined. According to the study, the optimum total nitrate and total phosphate concentrations required for the maximum algal growth were 200 mg L-1 and 10 mg L-1, respectively. The maximum tolerable Pb2+ and Cd2+ concentrations were 8 mg L-1 and 2.5 mg L-1, respectively. The results revealed that Chlorellavulgaris has a high capacity for both removing these ions (> 70%) from the growth medium, and generating algal biomass, simultaneously. Moreover, the algal biomass generated under these harsh conditions yields algal-oil (~41% in terms of dry algal biomass) that is free from these ions.

Assessing post-fire water quality changes in reservoirs: Insights from a large dataset in Portugal

Wildfires in the Mediterranean basin, especially in Portugal, have increased in extent and frequency over the last few years. One of the impacts of wildfires on humans and ecosystems is on the water quality of surface waters. Ashes and increased erosion rates might, for example, change oxygen levels and elevate the influx of sediments, nutrients, or other water quality-related components like metals and polycyclic aromatic hydrocarbons (PAHs), possibly affecting water supply. In this study, time series of eight water quality parameters: biological- and chemical oxygen demand (BOD and COD), electrical conductivity (EC), total phosphorous (TP), nitrate (NO3−), total suspended sediments (TSS), dissolved oxygen (DO), and pH, were assessed via changepoint analysis to identify events of post-fire water contamination in over 60 Portuguese reservoirs. Further, possible fire, watershed, reservoir, and climate-related drivers were linked with the occurrence of these contamination events through logistic regression using generalized additive models. All measured parameters exhibited post-fire changes, with some being more frequently affected than others. The concentrations of TP, NO3−, and TSS showed a noticeable increase following 9.6 %, 12.6 %, and 13.6 % of all wildfires, respectively. Most changes fell into the unusually large fire seasons of 2003–2005 and 2017. The most significant impacts could be seen in southern reservoirs after the fire seasons of 2003–2005. The burned area ratio of the watershed was identified as the main driver of post-fire water contamination, while reservoir and climate-related characteristics like water levels also played a significant role in some parameters. Increased levels of suspended sediments were identified as a potential threat to water supply, especially when large wildfires coincide with drought-induced low reservoir water levels. The identification of post-fire water contamination events and their drivers from large datasets can inform water managers about potential threats to water supply.

Microbial removal of nutrients from anaerobic digestate: assessing product-coupled and non-product-coupled approaches.

Although anaerobic digestate contains >90% water, the high nutrient content of digestate makes it economically and technically intractable to treatment by existing wastewater treatment technologies. This study separately assessed the feasibility of nutrient removal from digestate by Rhizopus delemar DSM 905 and a culture of phosphate-accumulating organisms (PAOs). With Rhizopus delemar DSM 905, we investigated concomitant nutrient removal from digestate-supplemented medium and fumaric acid production, as a potentially economical strategy for digestate treatment. Following the cultivation of R. delemar DSM 905 in a fermentation medium containing 25% (v/v) digestate, the concentrations of Al, Cr, Cu, Fe, K, Mg, Mn, Pb, and Zn reduced 40, 12, 74, 96, 12, 26, 23%, ~18, and 28%, respectively. Similarly, the concentrations of total phosphorus, total nitrogen, phosphate (PO4-P), ammonium (NH4-N), nitrate (NO3-N), and sulfur decreased 93, 88, 97, 98, 69, and 13%, respectively. Concomitantly, cultures supplemented with 25 and 15% (v/v) digestate produced comparable titers of fumarate (~11 and ~ 17 g/L, respectively) to the digestate un-supplemented control cultures. With PAOs, we assessed the removal of total phosphorus, total nitrogen, PO4-P, and NH4-N, of which the concentrations reduced 86, 90%, ~99, and 100%, respectively in 60% (v/v) digestate. This study provides additional bases for microbial removal of excess nutrients from anaerobic digestate, with the potential to engender future water recovery from this waste stream that is currently largely recalcitrant to treatment.

Water Quality Measurements with TSS, TDS, Total Phosphate, Nitrate, Total Coliform Parameters in the Garang Water Shed

Massive industrial and urban development can cause changes in the quality of river water, one of which is the Garang Watershed, located in Central Java Province. This river is situated in Kendal Regency (upper and middle) and Semarang City (middle and lower). This research examined the Garang River's water quality conditions, divided into four sub-basins: Garang Hilir, Garang Hulu, Kreo and Kripik. The parameter assessed in this research is TSS, TDS, Total Phosphate, Nitrate, and Total Coliform Parameters with a total of ten sampling location points were determined based on several criteria using GPS and the SNI 6989.57:2008 sampling method. TSS and TDS concentrations in March-April were observed to be high, especially at sampling point 10. In contrast, total phosphate concentrations were elevated at sampling points 2, 3, and 9; Nitrate values were observed to be high at sampling point 3 in March and sampling point 5 in April; Total coliforms were high at sampling points 1, 3, and 4, all of these is possibly due to domestic waste and the use of fertilizer by agricultural activities. This sampling quality assessment shows a close relationship between human activities and agricultural activities in river water quality, especially in the Garang River.

Cordymin alleviates osteoporosis induced by hindlimb unloading via regulating the gut - microelements -bone axis --for non-clinical studies

IntroductionThe purpose of this study was to evaluate the protective effects of cordymin on osteoporosis induced by hindlimb unloading(HLU) in rats and whether cordymin can prevent bone loss from HLU.Materials and methodsWe employed the hindlimb suspension rats model to mimic physiological changes concomitant with space travel.The mechanical strength in the femoral neck,cancellous bone volume, gut microbiota structure,serum calcium and phosphorus contents, bone mineral content and bone mineral content can be changed after hindlimb unloading. Oral cordymin was administered for 4 weeks,cordymin treatment significantly increased the mechanical strength through elevated bone volume/tissue volume (BV/TV), trabecular number (Tb. N), trabecular thickness (Tb. Th) and decreased trabecular separation (Tb. Sp).ResultsImportantly, 16 S rRNA sequencing showed cordymin treatment regulated the various genera that were imbalanced in hindlimb unloading rats. At the same time,The plasma total calcium and inorganic phosphate concentrations in hindlimb unloading rats decreased and bone mineral content in the lumbar vertebrae and femur increased after treatment with cordymin.ConclusionThese data indicate that the cordymin might exert bone protective effects indirectly via modulating the complex relationship between gut microbiota, microelements and bone loss.