Concentrations Of Several Ions Research Articles

Distinct ecological processes shape micro-eukaryotic generalists and specialists in a canal-disturbed wetland, Lake Weishan, China

Micro-eukaryotic communities are highly sensitive to environmental changes, and their structure offers crucial insights into the impact of human activities on aquatic habitats. Despite their significance, the assembly mechanisms of micro-eukaryotic communities in lake ecosystems heavily influenced by human activities are not well understood. In this study, we utilized 18S rDNA amplicon sequencing to examine the biodiversity of micro-eukaryotes over a one-year period in Lake Weishan, one of the largest freshwater lakes in northern China. We analyzed the ecological processes governing community assembly among key ecological groups, specifically habitat generalists and specialists. Our findings revealed substantial temporal variation in microbial diversity and community composition, likely driven by water temperature, dissolved oxygen, and the concentrations of several ions (e.g., Ca2+ and F−). Notably, community assembly in this canal-disturbed lake was predominantly influenced by stochastic processes (58.18%) rather than deterministic processes (20.46%). Habitat specialists (425 operational taxonomic units, OTUs) exhibited significantly greater diversity compared to habitat generalists (189 OTUs) and were predominantly shaped by stochastic processes (52.47%). In contrast, habitat generalists were less influenced by these processes (17.69%). Altogether, these results suggest that the ecological processes governing micro-eukaryotic community assembly differ markedly between habitat generalists and specialists, primarily due to their varied adaptability to environmental changes.

Intracellular ion concentrations and cation-dependent remodelling of bacterial MreB assemblies

Here, we measured the concentrations of several ions in cultivated Gram-negative and Gram-positive bacteria, and analyzed their effects on polymer formation by the actin homologue MreB. We measured potassium, sodium, chloride, calcium and magnesium ion concentrations in Leptospira interrogans, Bacillus subtilis and Escherichia coli. Intracellular ionic strength contributed from these ions varied within the 130–273 mM range. The intracellular sodium ion concentration range was between 122 and 296 mM and the potassium ion concentration range was 5 and 38 mM. However, the levels were significantly influenced by extracellular ion levels. L. interrogans, Rickettsia rickettsii and E. coli MreBs were heterologously expressed and purified from E. coli using a novel filtration method to prepare MreB polymers. The structures and stability of Alexa-488 labeled MreB polymers, under varying ionic strength conditions, were investigated by confocal microscopy and MreB polymerization rates were assessed by measuring light scattering. MreB polymerization was fastest in the presence of monovalent cations in the 200–300 mM range. MreB filaments showed high stability in this concentration range and formed large assemblies of tape-like bundles that transformed to extensive sheets at higher ionic strengths. Changing the calcium concentration from 0.2 to 0 mM and then to 2 mM initialized rapid remodelling of MreB polymers.

ArrhythmoGenoPharmacoTherapy.

This review is focusing on the understanding of various factors and components governing and controlling the occurrence of ventricular arrhythmias including (i) the role of various ion channel-related changes in the action potential (AP), (ii) electrocardiograms (ECGs), (iii) some important arrhythmogenic mediators of reperfusion, and pharmacological approaches to their attenuation. The transmembrane potential in myocardial cells is depending on the cellular concentrations of several ions including sodium, calcium, and potassium on both sides of the cell membrane and active or inactive stages of ion channels. The movements of Na+, K+, and Ca2+ via cell membranes produce various currents that provoke AP, determining the cardiac cycle and heart function. A specific channel has its own type of gate, and it is opening and closing under specific transmembrane voltage, ionic, or metabolic conditions. APs of sinoatrial (SA) node, atrioventricular (AV) node, and Purkinje cells determine the pacemaker activity (depolarization phase 4) of the heart, leading to the surface manifestation, registration, and evaluation of ECG waves in both animal models and humans. AP and ECG changes are key factors in arrhythmogenesis, and the analysis of these changes serve for the clarification of the mechanisms of antiarrhythmic drugs. The classification of antiarrhythmic drugs may be based on their electrophysiological properties emphasizing the connection between basic electrophysiological activities and antiarrhythmic properties. The review also summarizes some important mechanisms of ventricular arrhythmias in the ischemic/reperfused myocardium and permits an assessment of antiarrhythmic potential of drugs used for pharmacotherapy under experimental and clinical conditions.

Metal ion-oxytetracycline pharmacokinetic interactions after oral co-administration in broiler chickens

The influence of the composition of calcium (Ca(2+)), magnesium (Mg(2+)), and iron (Fe(3+)) ions in two concentration levels (low-500 mg/L of CaCl2, 125 mg/L of MgCl2, and 10 mg/L of FeCl3 and high-2,500 mg/L of CaCl2, 625 mg/L of MgCl2, and 50 mg/L of FeCl3) contained in water on the pharmacokinetics (PK) of oxytetracycline (OTC) was determined. OTC hydrochloride was administered at a dose of 25 mg/kg of body weight to broiler chickens divided into four groups of nine birds each, including 3 oral groups (in deionized water -control, in water with low ion concentration, and in water with high ion concentration) and 1 intravenous group. OTC concentrations in plasma were determined using liquid chromatography-tandem mass spectrometry, after which non-compartmental pharmacokinetic analysis was conducted.The absolute bioavailability of OTC in the group of birds exposed to higher ions concentration was reduced (8.68% ± 2.56) as compared to the control (13.71% ± 2.60). Additionally, in this group, decrease in PK parameters such as: area under the concentration-time curve from 0 to infinity (15.36 μg × h/mL ± 4.36), from 0 to t (14.78 μg × h/mL ± 4.37), area under the first moment of curve from 0 to t (107.54 μg × h/mL± 36.48), and maximum plasma concentration (2.13 μg/mL ± 0.32) were also observed. It is noteworthy, all mentioned parameters demonstrated a downward trend with high correlation coefficient (P = 0.004, P = 0.002, P = 0.005, P = 0.004, P = 0.011, respectively), reflecting the influence of increasing concentrations of Ca(2+), Mg(2+), and Fe(3+) ions on the decreasing absorption rate of OTC.Based on the current research results, it can be assumed that high concentrations of several ions applied concomitantly are able to decrease the absorption of OTC from gastrointestinal tract in broiler chickens. This occurrence might impair the drug's clinical efficacy toward some pathogenic microorganisms. It implies that using OTC on a farm may require administration of higher doses than the routine one when infections are caused by less sensitive pathogens.

Chronic Severe Hyponatremia and Cardiopulmonary Bypass: Avoiding Osmotic Demyelination Syndrome

Serum sodium concentration affects every cell in the body with respect to cellular tonicity. Hyponatremia is the most frequent electrolyte abnormality encountered, occurring at clinical admission in 22% of elderly patients. Any rapid correction of chronic severe hyponatremia can result in rapid cellular shrinking due to loss of intracellular free water. This is commonly associated with paralysis and severe brain damage due to osmotic demyelination syndrome (ODS). ODS occurs because the body has the ability to compensate for cellular fluid shifts due to chronic hyponatremia (by a decrease in brain concentration of several ions, amino acids, and organic osmolytes). Thus, the neurons are often at a functional state of fluid balance despite the sodium imbalance. The initiation of cardiopulmonary bypass (CPB) can introduce between 1 and 2 L of priming solution containing a normal sodium concentration creating a rapid rise in sodium concentration within the extracellular fluid. This abrupt change establishes a situation where intracellular free water can be lost resulting in cellular shrinking and ODS. In presenting this case study, we hope to add to the current literature with a specific isotonic approach to treating the chronically severe hyponatremic patient pre-CPB, during CPB, and post-CPB.

Efficacy of regulation of the blood serum physicochemical parameters in children with dysfunction of physiological systems

The physicochemical parameters of blood serum (osmolality, concentration of several ions, total protein, glucose) were studied in 200 children of different age with various forms of pathology. The variability of each parameter was calculated. A high level of stability of the parameters studies was revealed in healthy children and in children with dysfunction of various systems (disease of the respiratory system, gastrointestinal tract, renal and urinary tract, nervous and endocrine systems). However, estimation of their coefficients of variation showed significant individual deviations of these parameters from the average value of the examined patients. This fact reflects the extent of efficacy of activity of different organs and regulatory systems under pathological processes. Combination of clinical and ontogenetic methods of evolutionary physiology in this study opens new possibilities for understanding the nature of regulation of water-salt balance in humans and points out to the expedience of using these approaches in the practical medicine.

Does Na+/Ca2+ Exchanger, NCX, Represent a New Druggable Target in Stroke Intervention?

Stroke causes a rapid cell death in the core of the injured region and triggers mechanisms in surrounding penumbra area that leads to changes in concentrations of several ions like intracellular Ca²⁺, Na⁺, H⁺, K⁺, and radicals such as reactive oxygen species and reactive nitrogen species. When a dysregulation of homeostasis of these messengers occurs, it can trigger cell death. In particular, it is widely accepted that a critical factor in determining neuronal death during cerebral ischemia is progressive dysregulation of Ca²⁺, Na⁺, K⁺, and H⁺ homeostasis that activate several death pathways, including oxidative and nitrosative stress, mitochondrial dysfunction, protease activation, and apoptosis. In the last decade, several seminal experimental works are markedly changing the scenario of research of principal players of an ischemic event. Indeed, some plasma membrane channels and transporters, involved in the control of Ca²⁺, Na⁺, K⁺, and H⁺ ion influx or efflux and, therefore, responsible for maintaining the homeostasis of these four cations, might function as crucial players in initiation of brain ischemic process. Indeed, these proteins, by regulating ionic homeostasis, may provide the molecular basis underlying glutamate-independent Ca²⁺ and Na⁺ overload mechanisms in neuronal ischemic cell death and, most importantly, may represent more suitable molecular targets for therapeutic intervention. Recently, a great deal of interest has been devoted to clarify the role of the plasma membrane protein known as Na⁺/Ca²⁺ exchanger, a transporter able to control Na⁺ and Ca²⁺ homeostasis. In this review, the pathophysiological role of NCX and its implication as a potential target in stroke intervention will be examined.

Using Geospatial Techniques to Analyze Landscape Factors Controlling Ionic Composition of Arctic Lakes, Toolik Lake Region, Alaska

The impacts of climate change on landscapes in arctic Alaska are evident in terms of permafrost melting, frequent thermokarst activity, and the occurrence of more broadleaf vegetation. These changes may alter natural biogeochemical cycles of ions along with major nutrients and affect ionic compositions of lakes, as they are connected with the landscapes. However, the nature of the connectivity between lakes and landscapes in this region is not yet explored. The authors propose that geospatial analysis of landscape properties along with observed lake ion concentrations will enable an understanding of the currently existing landscape controls over ion inputs into the lakes. For the watersheds of 41 lakes in the Arctic Foothills region of Alaska, spatial properties of natural vegetation communities expressed in terms of percentage, shape complexity, and patch density metrics were derived using satellite data. Regression analyses were performed for concentration of ions as well as conductivity in lake water where the spatial metrics along with lake physical properties, lake order, and glacial till age categories were used as predicting variables in the regression. Landscape metrics for major land covers i.e., Percentage of Moist Acidic Tundra (MAT) and Moist Non-acidic Tundra (MNT) were the major predicting variables for concentration of several ions.

Metabolic impact of a soccer match on female players

The aim of this study was to examine the metabolic effect of a female soccer match in elite and sub-elite teams. Blood samples were taken (24 h before, immediately after and 18 h after official soccer matches) to determine haematological, electrolytic and hormonal variables, as well as the levels of cell damage and oxidative stress in 14 elite and 14 sub-elite players. Our results show increases in white blood cell count (P < 0.001) and the percentage of neutrophils (P < 0.001), and decreases in the percentage of lymphocytes (P < 0.05), eosinophils (P < 0.001), monocytes (P < 0.001) and basophils (P < 0.05) immediately post-match. Increases were also found in lactate dehydrogenase activity (P < 0.001), uric acid (P < 0.001), albumin (P < 0.001), total antioxidant status (P < 0.001) and free testosterone levels (P < 0.01). Creatine kinase activity significantly increased 18 h post-match (P < 0.01) and the concentrations of several ions, glucose and proteins were found to be altered immediately post-match. Overall, our results show that playing a soccer match exerts specific metabolic effects on female players, resulting in muscle damage, oxidative stress and biochemical and hormonal variations. On the basis of some interesting correlations, we also suggest that exercise-induced cell breakdown may enhance antioxidant capacity of the soccer players.

Do multiple ionic interactions contribute to skeletal muscle fatigue?

During intense exercise or electrical stimulation of skeletal muscle the concentrations of several ions change simultaneously in interstitial, transverse tubular and intracellular compartments. Consequently the functional effects of multiple ionic changes need to be considered together. A diminished transsarcolemmal K(+) gradient per se can reduce maximal force in non-fatigued muscle suggesting that K(+) causes fatigue. However, this effect requires extremely large, although physiological, K(+) shifts. In contrast, moderate elevations of extracellular [K(+)] ([K(+)](o)) potentiate submaximal contractions, enhance local blood flow and influence afferent feedback to assist exercise performance. Changed transsarcolemmal Na(+), Ca(2+), Cl(-) and H(+) gradients are insufficient by themselves to cause much fatigue but each ion can interact with K(+) effects. Lowered Na(+), Ca(2+) and Cl(-) gradients further impair force by modulating the peak tetanic force-[K(+)](o) and peak tetanic force-resting membrane potential relationships. In contrast, raised [Ca(2+)](o), acidosis and reduced Cl(-) conductance during late fatigue provide resistance against K(+)-induced force depression. The detrimental effects of K(+) are exacerbated by metabolic changes such as lowered [ATP](i), depleted carbohydrate, and possibly reactive oxygen species. We hypothesize that during high-intensity exercise a rundown of the transsarcolemmal K(+) gradient is the dominant cellular process around which interactions with other ions and metabolites occur, thereby contributing to fatigue.

Cell Viability and Prostacyclin Release in Cultured Human Umbilical Vein Endothelial Cells

Construction of efficient substitutes of human blood vessels is strongly dependent on the use of viable and fully functional cultured endothelial cells (ECs). However, very few reports have been published to date focused on the evaluation of cell viability of cultured ECs. In this work, we have determined cell viability, von Willebrand factor, and prostacyclin (PGI(2)) activity in primary cell cultures of human umbilical vein ECs, to identify the specific cell passage that is more appropriate for the development of artificial organs by tissue engineering. Cell viability was determined by quantification of the intracellular concentration of several ions by highly sensitive electron probe X-ray microanalysis, whereas von Willebrand was assayed by immunohistochemistry and PGI(2) release was quantified by radioimmunoassay. The results of our analyses demonstrate that the K/Na ratio was different for each cell passage (4.72 for the first passage, 4.55 for the second passage, and 7.82 for the third passage), suggesting that the highest cell viability corresponds to the third passage. In contrast, PGI(2) production was higher at the first two cell passages, with a significant decrease at the third passage (6.46 +/- 0.10, 5.98 +/- 0.08, and 1.62 +/- 0.05 ng/mL of supernatant for the first, second, and third passages, respectively), whereas von Willebrand expression was similar among the three cell passages analyzed in this work (64.12%, 66.66%, 65.93% of positive cells, respectively). These data suggest that cells corresponding to the second cell passage show the best ratio of viability to functionality and should therefore be used for tissue engineering protocols.

Temporal evolution of salts in Mediterranean soils transect under different climatic conditions

The research was conducted in Israel at three sites along a south–north axis, characterized by increasing annual rainfall, from 310 mm at site LAV in the south through 600 mm at site MAT (600), to 800 mm at site EIN in the north. At each site soil samples were taken during several seasons (September 2001 through April 2003), in three dominant microenvironments at 0–2 cm and 5–10 cm. The following microenvironments were selected at LAV and MAT: “Under Shrub” (US), “Between Shrubs” (BS), and “Under Rock fragments” (UR). At EIN the selected microenvironments were US, BS, and “Under Tree” (UT). In each soil sample electrical conductivity (EC), pH, and concentrations of several ions were determined. The objective was to analyze the effects of soil microenvironments and climatic conditions on the temporal dynamics of salt concentrations. In all microenvironments at all sites the minimal values of EC were found in the rainy season (January or April), and the maximal values in the dry season (September). In the rainy season the temporal variability of EC in the topsoil was regulated by: (1) clay, which restricted the leaching of salts from the topsoil when EC was low; and (2) surface features (microenvironment), when EC was high. In the UT, US, and UR microenvironments the rainy season could be divided into two periods with respect to their effect on salt movement in the topsoil: at the beginning of the rainy season (September–January) the reduction in EC was relatively moderate, especially with regard to ions involved in biotic activity (Mg ++ and K +), whereas, late in the rainy season (January–April) there was enhanced reduction in EC. In contrast, in BS the regulation of salt movement was weak at all sites. Hence, in this microenvironment the salts concentration (mainly Na + and Cl −) responded rapidly to changes in rain amount and soil moisture and temperature. In the dry season (April–September) the temporal variation in EC varied not only between microenvironments but also between sites. In US, where local surface features were similar at all sites (the same shrub), the rise in EC was maximal at LAV (mainly Ca ++ and Na +), and gradually diminished toward EIN. Thus, the contribution of regional sources to the salts added to the soil diminished toward the humid site, EIN, where the EC hardly changed in any microenvironment. In BS and UR microenvironments the rise in EC (mainly in Ca ++, Na +, and K +) was greatest at site MAT, and decreased toward LAV and EIN. It seems that this pattern was affected also by changes in local biotic activity.

Using geochemical data and modelling to enhance the understanding of groundwater flow in a regional deep aquifer, Aquitaine Basin, south-west of France

In deep aquifers the complex flow pattern originating from the geological structure often leads to difficult predictions of water origin, determination of the main flow paths, potential mixing of waters. All these uncertainties prevent an efficient management of the resource. In the context of the Aquitaine basin an original modelling approach suggests that geochemical data can be used to identify flow directions where geological and hydrogeological data are too scarce to provide sufficient information. In the Eocene sands aquifer, the major patterns of groundwater geochemistry suggest the presence of two distinct areas within the aquifer. In the north and the east, waters exhibit sodium bicarbonate or sodium sulphate facies, and moderate total dissolved solids related to high sulphate concentrations. In the south, waters are characterised by calcium bicarbonate facies and low total dissolved solids. Sulphur isotopic ratios provided key information on the origin of sulphur in solution (meteoric, gypsum dissolution, pyrite oxidation) and also on the intensity of the geochemical processes involved in the dissolution of minerals and the concentration evolution. A geochemical model was developed to analyse the processes generating the chemical composition of each sampled water. At the aquifer scale, four main geochemical processes—dissolution, redox, acid–base reaction, exchange—of varying intensity could explain most of the observed spatial variability in groundwater composition. Among several potential reaction schemes at each point, only one allowed to reproduce the independent variables (pH and 13C). The developed model was used to select the most probable water pathways at the aquifer scale. In this context, geochemistry clearly demonstrates the role played by subsurface structures on water flow velocities and residence time in their vicinity. In addition, the concentrations of several ions could only be justified by the aquitard–aquifer interactions.

Inhibition of a ubiquitously expressed pectin methyl esterase in Solanum tuberosum L. affects plant growth, leaf growth polarity, and ion partitioning.

Two pectin methyl esterases (PMEs; EC 3.1.1.11) from Solanum tuberosum were isolated and their expression characterised. One partial clone ( pest1) was expressed in leaves and fruit tissue, while pest2 was a functional full-length clone and was expressed ubiquitously, with a preference for aerial organs. Potato plants were transformed with a chimeric antisense construct that was designed to simultaneously inhibit pest1 and pest2 transcript accumulation; however, reduction of mRNA levels was confined to pest2. The decrease in pest2 transcript was accompanied by up to 50% inhibition of total PME activity, which was probably due to the reduction of only one PME isoform. PME inhibition affected plant development as reflected by smaller stem elongation rates of selected transformants when compared with control plants, leading to a reduction in height throughout the entire course of development. Expansion rates of young developing leaves were measured simultaneously by two displacement transducers in the direction of the leaf tip (proximal-distal axis) and in the perpendicular direction (medial-lateral axis). Significant differences in leaf growth patterns were detected between wild-type and transgenic plants. We suggest that these visual phenotypes could be correlated with modifications of ion accumulation and partitioning within the transgenic plants. The ion-binding capacities of cell walls from PME-inhibited plants were specifically modified as they preferentially bound more sodium, but less potassium and calcium. X-ray microanalysis also indicated an increase in the concentration of several ions within the leaf apoplast of transgenic plants. Moreover, quantification of the total content of major cations revealed differences specific for a given element between the leaves of PME-inhibited and wild-type plants. Reduced growth rates might also be due to effects of PME inhibition on pectin metabolism, predominantly illustrated by an accumulation of galacturonic acid over other cell wall components.

Effects of Tsuga canadensis mortality on soil water chemistry and understory vegetation: possible consequences of an invasive insect herbivore

Soil water chemistry and vegetation were monitored for almost 1 year before and 4 years after treatment in two healthy Tsuga canadensis (L.) Carrière (eastern hemlock) stands and two T. canadensis stands subjected to a girdling treatment. The girdling treatment was applied to simulate infestation and mortality by the introduced pest Adelges tsugae Annand. The girdling of T. canadensis trees resulted in elevated concentrations of NO3– and most cations in soil water within 2–3 months, and concentrations of several ions remained high relative to control stands for the duration of the study (e.g., NO3–, Ca2+, K+, Mg2+). Maximum mean monthly concentrations were generally observed 12–18 months after girdling (e.g., 473–2272 µequiv. NO3–/L, 22–126 µequiv. NH4+/L). Maximum mean annual weighted ion concentrations and fluxes (e.g., 236 µequiv. NO3–/L and 18 kg NO3–-N/ha, respectively) were observed 2–3 years after girdling and were comparable to values reported in the literature regarding clear-cutting effects on stream water ion concentrations and losses. Betula alleghaniensis Britt. seedling densities and percent cover of several herbaceous species (Dennstaedtia punctilobula (Michx.) Moore, Dryopteris intermedia (Muhl.) A. Gray, Oxalis acetosella L., Thelypteris noveboracensis (L.) Nieuwl.) increased in response to T. canadensis mortality. Total percent cover of understory vegetation more than doubled in the first 3 years after girdling.

Mars Surveyor Program '01 Mars Environmental Compatibility Assessment wet chemistry lab: a sensor array for chemical analysis of the Martian soil.

The Mars Environmental Compatibility Assessment (MECA) instrument was designed, built, and flight qualified for the now canceled MSP (Mars Surveyor Program) '01 Lander. The MECA package consisted of a microscope, electrometer, material patch plates, and a wet chemistry laboratory (WCL). The primary goal of MECA was to analyze the Martian soil (regolith) for possible hazards to future astronauts and to provide a better understanding of Martian regolith geochemistry. The purpose of the WCL was to analyze for a range of soluble ionic chemical species and electrochemical parameters. The heart of the WCL was a sensor array of electrochemically based ion-selective electrodes (ISE). After 20 months storage at -23 degrees C and subsequent extended freeze/thawing cycles, WCL sensors were evaluated to determine both their physical durability and analytical responses. A fractional factorial calibration of the sensors was used to obtain slope, intercept, and all necessary selectivity coefficients simultaneously for selected ISEs. This calibration was used to model five cation and three anion sensors. These data were subsequently used to determine concentrations of several ions in two soil leachate simulants (based on terrestrial seawater and hypothesized Mars brine) and four actual soil samples. The WCL results were compared to simulant and soil samples using ion chromatography and inductively coupled plasma optical emission spectroscopy. The results showed that flight qualification and prolonged low-temperature storage conditions had minimal effects on the sensors. In addition, the analytical optimization method provided quantitative and qualitative data that could be used to accurately identify the chemical composition of the simulants and soils. The WCL has the ability to provide data that can be used to "read" the chemical, geological, and climatic history of Mars, as well as the potential habitability of its regolith.

Early growth of seven mangrove species planted at different elevations in a Thai estuary

This study aimed to determine the effect of elevation on survival and growth of mangrove seedlings during the establishment period. Seven typical mangrove species, Rhizophora mucronata Poir, R. apiculata Bl., Bruguiera cylindrica (L.) Bl., Ceriops tagal (Pirr.) C.B. Rob., Sonneratia alba J. Sm., Avicennia officinalis L. and Xylocarpus granatum Koenig were planted at various topographic sites in an intertidal zone of Phang Nga province, southern Thailand. These six bare areas included two that were abandoned after tin mining and four gap areas in natural habitats in June 1998. The experimental plots were on a slope and showed a maximal elevation difference of 1.8 m. The plots were naturally submerged with 2–3% saline water twice a day. Salinity, pH and the concentrations of several ions in the soil water were similar in all the plots. Survival and growth performance of seedlings were measured every 6 months. Many seedlings of B. cylindrica, C. tagal and X. granatum planted at lower elevations died with a year. R. mucronata and S. alba survived even at the lowest elevations, but showed changes in growth rate in response to topography. Thus, early growth of the seven mangrove species at different elevations differed and showed increasing tolerance to higher tidal inundations in the order: R. mucronata, S. alba, R. apiculata, A. officinalis, C. tagal, B. cylindrica and X. granatum. These findings provide guideline information for appropriate species selection in a mangrove rehabilitation program.

Ion leaching before and after clear-cutting in a Norway spruce stand — effects of long-term application of ammonium nitrate and superphosphate

Soil solution chemistry was measured in a Haplic Podzol in a Norway spruce (Picea abies, L.Karst) site in central Sweden that had received frequent low-dose applications of nitrogen (N) asammonium nitrate (30-120 kg N/ha yr) and phosphorus (P) as superphosphate (20-40 kg P/haabout every third year) over a long period (22 yr). The aim was to investigate effects of added Non the ion leaching before and after clear-cutting. The N load corresponded well with actual loadsin central Europe. Prior to clear-cutting, fertilization increased concentrations of several ions inthe soil solution. Total losses of NO 3 - , Al andH+ increased with the intensity of fertilization. However, since fertilization enhanced tree growthand nutrient uptake, differences between the total losses of several other ions tended to beequalized. After clear-cutting, the pH in the soil solution decreased, and the losses of Mn and Alincreased with fertilizer load, probably because of increased N mineralization and nitrification. Inthe N treatments the concentration of SO 4 2- in the soil solution decreased, probably owing toincreased adsorption in the B horizon in response to the pH decrease. The effects ofanthropogenic N deposition are discussed in the light of our findings and possible causes oftemporal and spatial variation in soil solution chemistry and ion fluxes are suggested.

Rapid Induction of Ion Pulses in Tomato, Cucumber, and Maize Plants following a Foliar Application of L(+)-Adenosine.

Application of picomole quantities of (+)-adenosine, a plant growth-regulating second messenger elicited by triacontanol, to tomato (Lycopersicon esculentum Mill.), maize (Zea mays L.), and cucumber (Cucumis sativa L.) foliage, increased Ca2+, Mg2+, and K+ concentrations in the exudate from the stumps of excised plants by 20 to 60% within 5 s after treatment. The change in ionic concentration of the exudate was transitory. When L(+)-adenosine and triacontanol were applied to different tomato plants at the same time, the L(+)-adenosine caused an increase in Ca2+ flux within 3 s, whereas a significant increase from triacontanol was not detectable until 5 min after application. This was expected because triacontanol elicits the formation of L(+)-adenosine. The enantiomer of L(+)-adenosine, D(-)-adenosine, had no effect on the cation concentration in tomato and inhibited the effect of L(+)-adenosine at equimolar or lower concentrations. These observations suggest that L(+)-adenosine acts by eliciting a rapidly propagated signal that increases the concentration of several ions in the apoplast. We postulate that modulations in apoplastic ion concentration, especially increases in Ca2+ concentration, constitute a mechanism by which plants regulate metabolic activity and growth in response to certain stimuli.

Feed-back inhibition of milk secretion: the effect of a fraction of goat milk on milk yield and composition.

A milk fraction containing whey proteins of 10-30 kDa was injected into one mammary gland of lactating goats via the teat canal. This fraction produced a temporary dose-dependent reduction in milk yield in the treated gland; the milk yield of the other gland, which received an equal volume of carrier solution, was not affected. Injection of a second fraction, containing whey proteins of greater than 30 kDa, affected milk secretion only at high doses, and this effect was not wholly specific to the treated gland. The 10-30 kDa fraction and the greater than 30 kDa fraction produced similar transient changes in the concentrations of several ions and lactose in milk of the treated gland, but not in that of the untreated gland. These data indicate that a milk constituent present in the 10-30 kDa whey inhibits milk secretion in a temporary and reversible manner. The results are discussed in relation to regulation of milk secretion through local feedback inhibition.