Concentrations Of Organochlorine Compounds Research Articles

Distribution of polychlorinated biphenyls and organochlorine pesticides in human breast milk from various locations in Tunisia: Levels of contamination, influencing factors, and infant risk assessment

The concentrations of dichlorodiphenytrichloroethane and its metabolites (DDTs), hexachlorobenzene (HCB), hexachlorocyclohexane isomers (HCHs), dieldrin, and 20 polychlorinated biphenyls (PCBs) were determined in 237 human breast milk samples collected from 12 locations in Tunisia. Gas chromatography with electron capture detector (GC-ECD) was used to identify and quantify residue levels on a lipid basis of organochlorine compounds (OCs). The predominant OCs in human breast milk were PCBs, p, p′-DDE, p, p′-DDT, HCHs, and HCB. Concentrations of DDTs in human breast milk from rural areas were significantly higher than those from urban locations ( p<0.05). With regard to PCBs, we observed the predominance of mid-chlorinated congeners due to the presence of PCBs with high K ow such as PCB 153, 138, and 180. Positive correlations were found between concentrations of OCs in human breast milk and age of mothers and number of parities, suggesting the influence of such factors on OC burdens in lactating mothers. The comparison of daily intakes of PCBs, DDTs, HCHs, and HCB to infants through human breast milk with guidelines proposed by WHO and Health Canada shows that some individuals accumulated OCs in breast milk close to or higher than these guidelines.

Retrospective determination of primary feeding areas of Atlantic salmon (Salmo salar) using fingerprinting of chlorinated organic contaminants

AbstractSvendsen, T. C., Vorkamp, K., Rønsholdt, B., and Frier, J-O. 2008. Retrospective determination of primary feeding areas of Atlantic salmon (Salmo salar) using fingerprinting of chlorinated organic contaminants. – ICES Journal of Marine Science, 65: 921–929. Atlantic salmon (Salmo salar) undertake extensive marine migrations. In the marine environment, the Atlantic salmon appears to suffer from heavy mortality, indicating the need for increased knowledge of its movements and habitat use at sea. Persistent organochlorine compounds (OCs) are found in measurable concentrations in all marine ecosystems. Geographically varying sources of OCs, transport, and transformation processes lead to different OC concentrations and compositions in the various ecosystems. As the principal source of organochlorine uptake in salmon is diet, populations utilizing different feeding areas may accumulate pollutant loads corresponding to their primary feeding areas. This hypothesis was tested by comparing the OC composition in Atlantic salmon from four locations: Lake Vättern (Sweden), Lake Vänern (Sweden), the Baltic Sea (off Denmark), and the River Imsa (Norway). Muscle and liver samples from each fish were analysed for 30 organochlorines (polychlorinated biphenyls, dichlorodiphenyltrichloroethanes, HCHs, hexachlorobenzene, and trans-nonachlor). Principal component analysis on normalized OC concentrations (OC pattern) showed separation of the salmon populations according to location; contaminant patterns were similar for liver and muscle tissue. It is therefore suggested that OC fingerprinting may be a valuable tool in identifying primary foraging areas of salmonids.

Organochlorine Pollutants and Stable Isotopes in Resident and Migrant Passerine Birds from Northwest Michoacán, Mexico

Although concentrations of organochlorine compounds (OCs) in birds from most of the United States and Canada have decreased over the last 30 years, there is still concern that migrant birds might be exposed to elevated concentrations of OCs during migration in Latin America. The Lerma-Chapala Basin in west-central Mexico is an important migration corridor and wintering area for many species. The objectives of this study were to assess if resident and migrant birds wintering in western Michoacán, Mexico accumulated elevated concentrations of OCs during fall and spring and to determine if the stable isotopes delta(15)N, delta(13)C, and deltaD could be used to predict burdens and origins of DDE accumulation. Resident and migrant passerine insectivorous birds were collected during fall and spring (2001-2002) in northwest Michoacán, near Chapala Lake, Mexico. The carcasses were analyzed for OCs and tail feathers were analyzed for stable isotopes delta(15)N, delta(13)C, and deltaD. The OCs detected in more than 50% of the samples were: oxychlordane (79%), p,p'-DDE (100%), p,p'-DDT (57%), and total PCBs (100%). p,p'-DDE was the OC detected at the highest concentrations, whereas residues of other OCs were near or below detection limits. Overall, there were no significant differences in concentrations of OCs between seasons or between resident and migrant birds. Concentrations of DDE and oxychlordane were somewhat higher in migrant and resident birds during spring than in fall; however, concentrations were significantly different only for oxychlordane. Two resident birds collected in fall and spring had DDE residues >10 microg/g wet weight in carcass. There were no significant differences in delta(13)C and delta(15)N values among species, between seasons, or between migrant and resident birds. However, deltaD values were clearly different between species and helped differentiate migrant from resident birds. deltaD values also were negatively and significantly correlated with DDE concentrations in carcass. Birds with more depleted deltaD values in feathers tended to have higher DDE concentrations than those with less depleted deltaD values, suggesting a potential latitudinal accumulation of DDE. Overall, our results suggest that during fall and spring, there is not a significant buildup of persistent OCs in migrant and resident passerine insectivorous birds in northwest Michoacán, Mexico.

CALCULATIONS REGARDING THE ORGANOCHLORINE PESTICIDES CONTAMINATION IN SOME SOUR-CREAM TYPES FROM THE BIHOR COUNTY

The paper presents the results of the tests from the contamination point of view of some sour-cream types with the same fat percentage, coming from some of the production units in the Bihor county. Qualitative and quantitative calculations have been performed through the gas-chromatographic method using capillary columns and electrode with electrons capture. The values have been obtained through comparison with a complex standard diluted at 50 ppb. The tests submitted to verification consisted of fat extracts purified through methods specific to determination in extents of ppm-ppb concentrations. The object of the study consisted in the verification of the incidence and concentration of organochlorine compounds under sanitary veterinary surveillance. After the determinations performed, we discovered, for most of the surveilled compounds, values under the approved limits, except for Endrine and Endosulphane.

Investigation of hydrophobic contaminants in an urban slough system using passive sampling – insights from sampling rate calculations

Semipermeable membrane devices (SPMDs) were deployed in the Columbia Slough, near Portland, Oregon, on three separate occasions to measure the spatial and seasonal distribution of dissolved polycyclic aromatic hydrocarbons (PAHs) and organochlorine compounds (OCs) in the slough. Concentrations of PAHs and OCs in SPMDs showed spatial and seasonal differences among sites and indicated that unusually high flows in the spring of 2006 diluted the concentrations of many of the target contaminants. However, the same PAHs - pyrene, fluoranthene, and the alkylated homologues of phenanthrene, anthracene, and fluorene - and OCs - polychlorinated biphenyls, pentachloroanisole, chlorpyrifos, dieldrin, and the metabolites of dichlorodiphenyltrichloroethane (DDT) - predominated throughout the system during all three deployment periods. The data suggest that storm washoff may be a predominant source of PAHs in the slough but that OCs are ubiquitous, entering the slough by a variety of pathways. Comparison of SPMDs deployed on the stream bed with SPMDs deployed in the overlying water column suggests that even for the very hydrophobic compounds investigated, bed sediments may not be a predominant source in this system. Perdeuterated phenanthrene (phenanthrene-d (10)). spiked at a rate of 2 microg per SPMD, was shown to be a reliable performance reference compound (PRC) under the conditions of these deployments. Post-deployment concentrations of the PRC revealed differences in sampling conditions among sites and between seasons, but indicate that for SPMDs deployed throughout the main slough channel, differences in sampling rates were small enough to make site-to-site comparisons of SPMD concentrations straightforward.

Association of Thyroid Hormone Concentrations with Levels of Organochlorine Compounds in Cord Blood of Neonates

BackgroundThyroid hormones are important regulators of brain development. During critical periods of development, even transient disorders in thyroid hormone availability may lead to profound neurologic impairment. Animal experiments have shown that certain environmental pollutants, including heavy metals and organochlorine compounds such as polychlorinated biphenyls (PCBs) and dioxins, can interfere with thyroid hormone homeostasis. Whether these contaminants can affect circulating levels of thyroid hormones in humans is unclear, however, because the results of available studies are inconsistent.ObjectivesThe aim of the present study is to examine the possible relationships between concentrations of environmental pollutants and thyroid hormone levels in human umbilical cord blood.MethodsWe measured concentrations of environmental pollutants [including selected PCBs, dioxin-like compounds, hexachlorobenzene, p,p′-DDE (dichlorodiphenyldichloroethylene), cadmium, lead] and thyroid hormones in the cord blood of 198 neonates.ResultsA statistically significant inverse relationship between concentrations of organochlorine compounds and levels of both free triiodothyronine (fT3) and free thyroxine (fT4), but not thyroid-stimulating hormone, was observed. We found no association between concentrations of heavy metals and thyroid hormone levels.ConclusionsOur results suggest that environmental chemicals may affect the thyroid system of human neonates. Although the differences in fT3 and fT4 levels associated with the organochlorine compounds were within the normal range, the observed interferences may still have detrimental effects on the neurologic development of the individual children, given the importance of thyroid hormones in brain development.

The influence of lipid and lifestyle factors upon correlations between highly prevalent organochlorine compounds in patients with exocrine pancreatic cancer

We aimed to analyse the influence of cholesterol and triglycerides, and of tobacco, coffee and alcohol consumption upon correlations between serum concentrations of organochlorine compounds (OCs) in patients with exocrine pancreatic cancer (EPC). Incident cases of EPC diagnosed in eastern Spain were prospectively identified (N=144). OCs were analysed by high-resolution gas chromatography with electron-capture detection. A strong correlation was observed between hexachlorobenzene (HCB) and β-hexachlorocyclohexane (β-HCH) (Spearman's ρ=0.758). β-HCH showed ρ>0.4 with p,p′-DDT, p,p′-DDE, PCB138 and PCB153 (all p<0.001). Some correlations among compounds were slightly affected by tobacco, coffee or alcohol consumption. We observed a striking diversity of correlation patterns by strata of cholesterol and triglycerides. Most correlations were higher in the lowest category of triglycerides than in the lowest category of cholesterol. Most coefficients above 0.7 were seen in the lowest category of triglycerides (e.g., OC pairs p,p′-DDT and HCB, p,p′-DDT and β-HCH, p,p′-DDE and β-HCH, or HCB and β-HCH). Correlations among OCs may be stronger when concentrations of triglycerides are low than when they are high. This is compatible with a dilution in the early phases of cancer and with a concentration effect as triglycerides become lower in the advanced phases of the disease.

Organochlorine Compounds in Trout from Lakes over a 1600 Meter Elevation Gradient in the Canadian Rocky Mountains

The effect of altitude on the concentration and composition of organochlorine compounds (OC) in troutwas investigated along an elevation gradient of 1600 m in the Canadian Rocky Mountains. The eight lakes sampled were within or adjacent to national parks in sparsely settled parts of Alberta and British Columbia, thus contaminants were assumed to have derived from long-range atmospheric transport. Concentrations of several OCs in trout increased significantly with lake elevation. In general, these increases were most pronounced for the higher K(ow) pesticides (i.e., dieldrin and DDTs), and less pronounced for lower K(ow) pesticides (e.g., HCHs and HCB) and PCBs. Most OC concentrations in trout were inversely correlated with fish growth rate. Growth rate explained more of the variation for some OCs (particularly PCBs) than lake elevation. Differences in trophic position (indicated by delta15N) explained little of the variation in OC concentration in comparison to other factors such as lake elevation and the growth rate and age of trout. Using principal component analysis (PCA), we identified the importance of lake elevation and octanol/water partition coefficient (K(ow)) to the OC composition of trout.

Determinants of serum concentrations of organochlorine compounds in Swedish pregnant women: a cross-sectional study.

BackgroundWe performed a cross-sectional study of associations between personal characteristics and lipid-adjusted serum concentrations of certain PCB congeners and chlorinated pesticides/metabolites among 323 pregnant primiparous women from Uppsala County (age 18–41 years) sampled 1996–1999.MethodsExtensive personal interviews and questionnaires about personal characteristics were performed both during and after pregnancy. Concentrations of organochlorine compounds in serum lipids in late pregnancy were analysed by gas chromatography. Associations between personal characteristics and serum levels of organochlorine compounds were analysed by multiple linear regression.ResultsParticipation rate was 82% (325 of 395 women). Serum concentrations of PCB congeners IUPAC no. 28, 52, 101, 105 and 167, and o, p'-DDT and -DDE, p, p'-DDT and -DDD, oxychlordane, and γ- and α-HCH were in many cases below the limit of quantification (LOQ). No statistical analysis of associations with personal characteristics could be performed for these substances. Concentrations of PCB congeners IUPAC no. 118, 138, 153, 156 and 180, HCB, β-HCH, trans-nonachlor and p, p'-DDE increased with increased age and were highest in women sampled early during the 4 year study period. This shows that older women and women sampled early in the study had experienced the highest life-time exposure levels, probably mainly during childhood and adolescence. The importance of early exposures was supported by lower PCB concentrations and higher β-HCH and p, p'-DDE concentrations among women born in non-Nordic countries. Moreover, serum concentrations of certain PCBs and pesticide/metabolites were positively associated with consumption of fatty fish during adolescence, and concentrations of CB 156, CB 180 and p, p'-DDE increased significantly with number of months women had been breast-fed during infancy. Short-term changes in bodily constitution may, however, also influence serum concentrations, as suggested by negative associations between concentrations of organochlorine compounds and BMI before pregnancy and weight change during pregnancy.ConclusionAlthough some of the associations could be caused by unknown personal characteristics confounding the results, our findings suggest that exposures to organochlorine compounds during childhood and adolescence influence the body burdens of the compounds during pregnancy.

Comparison of organochlorine compound concentrations in colostrum and mature milk

Human breast milk represents the best choice for the nutrition of infants. It is often used for monitoring human exposures to environmental chemicals. Uniquely suited to meet human biological needs, breast milk composition, especially fat content, changes significantly with time from delivery. With the aim to compare the concentration of organochlorine compounds (OCs) in colostrum versus mature milk, we obtained samples of fourth–fifth day postpartum milk (colostrum) and day-14 postpartum milk (mature milk) from 12 women enrolled in the project “Early Childhood Development and PCB Exposure in Slovakia”. The concentrations of selected organochlorine pesticides and congeners of polychlorinated biphenyls (PCBs) were measured using gas chromatography with electron capture detection and reported on lipid adjusted basis. No significant differences were found between organochlorine levels in colostrum and those in mature milk samples. A very close correlation was found between the concentrations of organochlorine compounds in colostrum and mature milk (Spearman correlation coefficient r = 0.94–0.98 for PCBs, and r = 0.85–0.99 for organochlorine pesticides, p < 0.001 for all compounds). The present study concludes that the use of colostrum samples provides a close estimate of the child’s exposure to OCs in the early neonatal period and demonstrates that, despite the lower fat content, colostrum specimens are adequate to conduct OC analyses.

In utero and post-natal accumulation of organochlorine compounds in children under different environmental conditions

A study of intake of organochlorine compounds (OCs) in children both in utero and at the age of four years has been performed in two cohorts. One encompasses children born in Menorca Island between 1997 and 1998, and the other those born between 1997-1999 in Ribera d'Ebre, an in-land industrial-agricultural area of Catalonia. Comparison of the OC concentrations in serum samples from both populations, including those obtained from cord blood and blood collected at four years, provides information on the influence of local pollution sources in the accumulation of these compounds. Statistically significant differences (p < 0.01) have been found for hexachlorobenzene, indicating that chronic airborne contamination to this compound in Ribera d'Ebre involved higher in utero exposure, which increased in the first four years of growth. Similarly, in the cohort of Menorca, higher in utero exposure to PCBs was observed and this increased subsequently in the first four years of growth. Other compounds encompassing temporal contamination episodes such as gamma-HCH involved higher in utero exposure but strongly diminished in the first four years of growth. Overall, it can be concluded that local chronic pollution by OCs has a direct effect in children living in the surrounding areas. This influence is reflected in higher exposure, both in utero and in the first years of growth, that significantly stands out over the background contamination due to the ubiquity of these compounds.

Plasma concentration of organochlorine compounds is associated with age and not obesity

It has been suggested that obese individuals, because of an increased dilution space (body fat) for lipophilic organochlorines compounds, may have greater levels of toxic pollutants than lean sedentary individuals. It is important to further examine this possibility because of the potential contribution of organochlorine pesticides in the development of Parkinson's disease and other neurological diseases. The aim of this study was to further investigate the relationship between the magnitude of obesity and the plasma concentration of organochlorines for a wide range of BMI (with participants at steady state body weight). Fifty-three individuals were selected on the basis of their body mass index (BMI): lean controls (n=16; mean BMI 22.8+/-2.2 kg/m(2); mean age 38.8+/-9.4 years), obese individuals (n=19; mean BMI 33.4+/-3.0 kg/m(2); mean age 38.6+/-7.6 years) and morbidly obese individuals (n=18; mean BMI 49.3+/-6.5 kg/m(2); mean age 44.3+/-9.2 years). Blood samples were analyzed for organochlorine compounds. The relationship between the total plasma organochlorine concentration and BMI was tested using a multiple regression analysis. Age was included in the model. There was no relationship between the total plasma organochlorine concentration and BMI. Organochlorine concentrations, however, were correlated with age (BMI-adjusted R(2)=0.46; p<0.001). At steady state body weight, toxic pollutant concentrations are not associated to obesity but strongly correlate with age.

Assessment of Prenatal and Early Postnatal Exposure to Organochlorine Compounds

P-481 Introduction: First exposure of children to environmental chemicals takes place in utero via transplacental transport. For the developing foetus, the mother represents both a source of genetic information and environmental exposure. Organochlorine compounds are persistent lipophilic compounds that cross the placental barrier easily and are also excreted into breast milk. The purpose of this study was to assess prenatal and early postnatal exposure to selected organochlorine compounds and to compare level of exposure among 3 different European regions. Methods: Within the 5th FP EU project PLUTOCRACY, a birth cohort was established in 5 localities in 3 European countries – Belgium, Slovakia and Romania. Samples of maternal blood sera and placentas were collected at full-term deliveries and breast milk samples were collected within the 6th week postpartum (N=464). Concentrations of selected organochlorine pesticides (HCB, β-HCH, p,p′ -DDE and p,p′ -DDT) and the 3 most abundant congeners of polychlorinated biphenyls (PCB #153, #138 and #180) were determined using GC/MS. Results were expressed per amount of lipid. Results: The most predominant compound was p,p′ -DDE in each of the biological matrices (median values/g lipid: serum: 312 ng, placenta: 213 ng, milk: 693 ng). Concentrations of organochlorine compounds were highest in breast milk samples, followed by maternal blood serum and placenta, except for HCB which reached highest levels in the blood serum (median 70 ng/g lipid). Significant correlations were found for organochlorine pesticides among maternal blood, placenta and breast milk, with the strongest associations for β-HCH (p< 0.001). PCB levels correlated between maternal blood and placenta and placenta and breast milk (p< 0.001), Higher maternal age was correlated with increased PCBs concentrations in each of the biological matrices (p< 0.001); however, this correlation was not observed for pesticides. Level of maternal education and parity were negatively associated with the concentrations of β-HCH, p,p′ -DDE and p,p′ -DDT (p< 0.001) in all sample types. Results suggest a wide variation in exposure to organochlorines across the regions, mainly with respect to pesticides. Concentrations of β-HCH, DDE and DDT were significantly higher in all biological samples from Romania, compared to Belgium and Slovakia (p< 0.001) but no clear trend was found for PCB exposure. Discussion and Conclusions: Organochlorine residues stored in the maternal body are continuously released into blood during pregnancy and into breast milk during lactation and are a major source of pre- and perinatal exposure.

Human milk, environmental toxins and pollution of our infants: Disturbing findings during the first six months of life

Background: Toxic organochlorine compounds (OC) are transmitted from mother to infant during lactation. OC are ingested by and stored in their offspring. Different harmful effects later in life have been attributed to the body pollution with these OC, although these findings are still discussed in an argumentative manner, since first other investigators could demonstrate beneficial effects of breast-feeding despite elevated OC concentrations, and second the benefits of breast-feeding are an unchallenged fact, especially in those countries, where infant formulas are not available. It was the aim of the present study to determine the lactational uptake of different OC (polychlorinated biphenyls (PCB), hexachlorobenzene (HCB), and DDE) in breast-fed vs. bottle-fed infants up to six months of age. Methods: With the written informed consent of the parents, blood samples were taken from each ten breast-fed and bottle-fed infants, respectively. The specimens were immediately centrifuged, and serum was stored in glass tubes without an anticoagulant up to analysis. Three higher-chlorinated PCB congeners (IUPAC Nos. 138, 153, and 180), HCB, and DDE, the main metabolite of DDT in mammals, were determined with capillary gas chromatography with electron capture detection. In addition, reliability was tested with gas chromatography-mass spectrometry. Possible correlations of OC with personal data were tested with a standard multivariate regression model. Differences between study groups were tested on mean differences with Wilcoxons test for independent samples. Results: We could demonstrate that breast-fed infants have significantly (p&lt;0.0001) elevated serum concentrations of all OC as early as at the age of six weeks (90 %), which over and above nearly doubled further until the age of six months. (Median [μg/L]; A = six weeks; B = six months): PCB 138, A: 0.40 vs. 0.09; B: 0.72 vs. 0.07; PCB 153, A: 0.57 vs. 0.11; B: 0.99 vs. 0.09; PCB 180, A: 0.33 vs. 0.04; B: 0.58 vs. 0.02; PCB (sum of the three PCB congeners), A: 1.19 vs. 0.29; B: 2.28 vs. 0.18; HCB, A: 0.13 vs. 0.04; B: 0.43 vs. 0.07; DDE, A: 1.05 vs. 0.18; B: 1.90 vs. 0.19. Conclusions: The discussion about the benefits of breast-feeding should be reconsidered again, with special emphasis on the question, whether the recommendations for breast-feeding can unreservedly be maintained for the future throughout the world, especially in face of the availability of infant formulas in industrialized vs. Third World countries, respectively.

Genotoxicity in wood mice (Apodemus sylvaticus) along a pollution gradient: Exposure‐, age‐, and gender‐related effects

We investigated the effects of environmental pollution on genetic damage in wood mice (Apodemus sylvaticus) by means of the comet assay, with special attention to the role of age and gender as potential confounding variables. The present study was carried out at four sites along a pollution gradient in the vicinity of Antwerp (Belgium), with a nonferrous smelter as the main pollution source. We measured the concentration of heavy metals (Cd, Co, Cr, Cu, Fe, Mn, Pb, and Zn) in mouse liver and kidney and the concentration of organochlorine compounds (polychlorinated biphenyls and 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene) in mouse muscle tissue to assess individual exposure. Cadmium exposure was very high at the sites closest to the smelter, and exposure to this metal decreased with increasing distance from the smelter. Exposure to the other pollutants was low to moderate at the different sites. Genetic damage was higher in mice from populations in the vicinity of the nonferrous smelter compared with that in the control populations. A significant increase in genetic damage with age was observed at the most polluted sites, but not at the control sites. Genetic damage was higher in male mice than in female mice at the most polluted site, but not at the other areas. Yet, no obvious relationship was found between individual pollutant levels and individual genetic damage levels. We conclude that the comet assay can be used to compare genotoxicity at the population level if the confounding variables of gender and age are taken into account. However, its use for individual health risk assessment remains questionable.

Elevated organochlorines in the brain–hypothalamic–pituitary complex of intersexual shovelnose sturgeon

Organochlorine compounds (OCs), including polychlorinated biphenyls and organochlorine pesticides, were used on lands adjacent to the Middle Mississippi River (MMR; USA) from 1930 through 1988, and they continue to occur in MMR fish. These compounds are estrogenic and/or antiandrogenic, and they alter hormone production and reception within the brain and gonads of male fish, resulting in intersexuality and/or suppressed gonadal development. To assess how OCs affect reproduction of MMR fish, we quantified OC accumulation, intersexuality, and gonadal development in male shovelnose sturgeon (Scaphirhynchus platorynchus) throughout the MMR during the spring of 2003. Gonads were observed for intersexual characteristics, weighed to calculate the gonadosomatic index (GSI), and examined histologically. Tissue accumulation of OCs was quantified in gonads, brain-hypothalamic-pituitary (BHP) complex, and fillets. Four of 48 mature males were identified macroscopically as intersexuals, and a fifth was found through histology (a 10.4% incidence). Intersexuals accumulated higher concentrations of OCs in the BHP complex compared with those of mature males. In addition, GSI and OC accumulation within the BHP complex, gonads, and fillets of mature males were negatively related. Exposure to OCs before or during sexual differentiation likely induces intersexuality in MMR shovelnose sturgeon, and exposure throughout gonadal maturation inhibits gonadal development.

Non-destructive pollution exposure assessment in the European hedgehog ( Erinaceus europaeus): IV. Hair versus soil analysis in exposure and risk assessment of organochlorine compounds

Few ecotoxicological studies on mammals use non-destructive methodologies, despite the growing ethical concern over the use of destructive sampling methods. In the present study we assessed exposure of hedgehogs ( Erinaceus europaeus) to polychlorinated biphenyls (PCBs), dichloro-diphenyl-trichloroethanes (DDTs), hexachlorocyclohexanes (HCHs) and hexachlorobenzene (HCB), by investigating concentrations of these compounds in soils and hedgehog hair from seven study sites around the urban area of Antwerp, Belgium. No relationships were observed between organochlorine compound concentrations in soils and hair from the different study areas. Furthermore, the individual variation of contamination levels in hair within study sites was high, especially for HCHs and HCB, and hair and soil had different relative profiles for PCBs, DDTs and HCHs. Our results show that concentrations of organochlorine compounds in soils alone are not predictive of the risk of these pollutants to hedgehogs and that tissue analyses are preferred to soil analyses in exposure and risk assessment studies.

Nationwide monitoring of polychlorinated biphenyls and organochlorine pesticides in sediments from coastal environment of Korea

To assess the organochlorine contamination in the Korean marine environment, a nationwide monitoring study was conducted. A total of 138 surface sediments, covering the whole Korean coast, were collected and analyzed. Organochlorine compounds (OCs) were widely distributed in the Korean coastal environment, with PCB and DDT contamination being particularly prevalent. The overall concentrations of PCBs, DDTs, HCHs, CHLs, and HCB in surface sediments were in the range of 0.088–199 ng g −1 (median value: 1.56 ng g −1), 0.006–135 ng g −1 (0.68 ng g −1), not detected (ND)–5.46 ng g −1 (0.32 ng g −1), ND–3.26 ng g −1 (0.14 ng g −1), and ND–2.59 ng g −1 (0.05 ng g −1), respectively. The southeastern coast was found to be highly contaminated. Overall contamination status of Korean coastal sediments with regard to OCs is lower than that of USA. With the exception of highly industrialized sites, Korean coastal areas in general showed similar OC concentrations to those of other Asian countries. There was a significant correlation between distributions of most organochlorine contaminants with each other. OC contamination is closely related to shipping and industrial activities. Of the 7 sites categorized as highly polluted, 4 are in a harbor zone. Adverse effects to benthic communities are expected at the levels of OC contamination observed from harbor and industrial areas.

Physical-chemical and Maternal Determinants of the Accumulation of Organochlorine Compounds in Four-Year-Old Children

A cohort study representing a general population (Minorca Island, birth year 1997-1998) showed that in utero transfer of organochlorine compounds (OCs) in children was strongly correlated with the age of the mother and, in the case of hexachlorobenzene (HCB), 4,4'-DDE, and 4,4'-DDT, with the maternal body mass index. Some of these correlations remained significant for the serum concentrations collected in these children at four years. No significant correlations with length of gestation were observed. Breastfeeding and age of lactation were strong determinants of most OC concentrations at four years of age. At this age, the body burden of these compounds was higher than at birth irrespective of maternal or formula feeding, but they accumulated at higher extent in the former case involving concentration increments in blood that surpassed the growth dilution effects, with the only exceptions being pentachlorobenzene (PeCB), HCB, and 4,4'-DDT. 4,4'-DDE exhibited the highest increase in association with breastfeeding, pointing to a specific accumulation pathway via this mode. Compounds with low K(ow) values such as beta-hexachlorocyclohexane showed significant accumulation in four-year-old children but with small differences between the groups that had been raised on either breast milk or formula. Compounds having low K(oa) values such as HCB showed decreases in concentration and small body burden variation between birth and four years of age, which points to their preferential elimination in these initial periods of infant growth.

Nondestructive pollution exposure assessment in the european hedgehog (Erinaceus europaeus): III. hair as an indicator of endogenous organochlorine compound concentrations

Concentrations of organochlorine persistent pollutants were investigated in tissues of the European hedgehog (Erinaceus europaeus). Road kills and carcasses from wildlife rescue centers were used to characterize organochlorine compound tissue distribution and tissue profile dissimilarities (hair, liver, kidney, muscle, and adipose tissue). The most important contaminants were polychlorinated biphenyls (PCBs), chlordanes (CHLs), and DDTs, with median concentrations of 75, 5.1, and 1.4 ng/g liver wet weight, respectively. Median levels for the remaining compounds-hexachlorobenzene (HCB), hexachlorocyclohexanes (HCHs), and octachlorostyrene-were less than 0.5 ng/g liver wet weight. Compared to results with other mammalian wildlife, the present results indicate that hedgehogs may accumulate considerable concentrations of organochlorine compounds. Polychlorinated biphenyls and HCB preferably accumulated in liver and muscle tissue. Concentrations of DDTs and HCHs were highest in muscle tissue and hair, respectively. Octachlorostyrene and CHL levels were predominant in liver. The observed positive relationships between concentrations in hair and internal tissues for PCBs, DDTs, HCB, HCHs, and CHLs (0.49 < r < 0.91) and for several individual PCB, DDT, and CHL compounds (0.31 < r < 0.76) indicate the usefulness of hair as a biomonitoring tool of organochlorine compounds. Multivariate profile analyses revealed a higher dominance of less-persistent compounds (p,p'-DDT, alpha-HCH, and PCBs 95, 101, and 149) in hair compared to internal tissues. The present study demonstrates the suitability of hedgehog hair as a nondestructive biomonitoring tool regarding pollution with organochlorine compounds and the promising role of the hedgehog as a mammalian indicator species of pollution in terrestrial environments.