Gel Concentration Research Articles

The effectiveness of varying of Tideglusib gel concentrations in ridge preservation of osteoporotic models

Background: Repairing impaired hard tissues in osteoporotic patients posed challenges with restricted treatment modalities. Meanwhile, Tideglusib is regarded as an exciting treatment for Alzheimer's disease; its applications for ridge preservation in osteoporotic rabbits are not investigated. The aim of this research was to investigate the effects of Tideglusib gel in osteoporotic rabbits with empty sockets. Thirty-two male rabbits weighing 2.5 - 3 kg were used for the study. Then the mandibular first molar of each rabbit was extracted. The rabbits were allocated into 4 study groups: 1. Control normal rabbit with socket healed normally; 2. Osteoporotic rabbit with socket healed normally; 3. Osteoporotic rabbit with socket received 5 μM Tideglusib gel; 4. Osteoporotic rabbit with socket received 10 μM Tideglusib gel. At four weeks, the rabbits were then slaughtered. To assess the bone development in the extraction sockets, histopathologic and histomorphometeric analysis were carried out.Results: After 4 weeks of socket augmentation, a significant increase in mean % of mature bone (MB) in the control group and two treated groups. While Group 2 showed the significantly lowest mean % increase in MB. Also, a significantly mean % increase of collagen deposition was generated in all study groups. However, group 2 showed the statistically significantly lowest mean % increase. Group 4 demonstrated significantly higher new bone formation than those of groups 2 and 3.Conclusion: Tideglusib gel could accelerate bone healing through an increase in the trabecular area and elevate collagen deposition in the extracted socket of a rabbit. A specific dose-dependent response of Tideglusib gel was observed in histological and morphometric parameters, indicating that 10 μM of Tideglusib gel is considered an effective rational therapy for post-extraction socket repair, particularly in the osteoporotic model.

Preparation of Ethosome Gel with Total Flavonoids from Vernonia anthelmintica (L.) Willd. for the Treatment of Vitiligo.

Vernonia anthelmintica (L.) Willd. is a traditional medicinal herb in Chinese medicine, extensively used by various ethnic groups due to the numerous advantages derived from its total flavonoids. These benefits encompass anti-inflammatory and antioxidant effects, and the promotion of melanin production, showcasing its significant efficacy in addressing vitiligo. To improve transdermal absorption and enhance the antioxidant effectiveness of the treatment, ethosome containing total flavonoids were prepared utilizing the ultrasound injection technique. The resulting ethosome was then carefully mixed with 0.7% Carbomer 934 gel in equal parts, yielding a gel concentration of 0.302 mg/g. This formulation produced small, consistent ethosome that exhibited high encapsulation efficiency and notable stability. In vitro analyses demonstrated sustained release characteristics of the gel and considerable therapeutic effectiveness against vitiligo resulting from hydroquinone exposure. Histological examinations performed through hematoxylin and eosin (H&E) staining of mouse skin revealed increased melanin production and increased activities of tyrosinase (TYR), cholinesterase (CHE), and mouse monoamine oxidase (MAO), while levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were reduced. These findings underscore the promising effectiveness of this treatment strategy and validate the efficacy of the dosage form.

Techno-functionality of jack bean (Canavalia ensiformis) protein concentrate: a comparative study with soy and pea proteins.

With the growing human awareness of the environmental and animal stress caused by the meat industry, the consumption of plant-based products has expanded. Plant proteins have gained market prominence due to their sustainable origin, economic value and health benefits. Well-established plant proteins in the market, such as those of soy and pea, have various applications as ingredients in the food industry. However, given the wide variety of protein sources, it is necessary to conduct studies on the chemical and techno-functional characterization of other raw materials to further diversify their properties. In this context, the present study introduces jack bean protein concentrate (JBPC) as a potential alternative to proteins already established in the market. Techno-functional properties such as surface hydrophobicity, solubility, zeta potential, water- and oil-holding capacity, foam capacity and stability, emulsion stability and gel formation and rheology were analyzed. The protein content obtained from the extraction of the JBPC was 73 g (100 g)-1 on a dry weight basis, with an extraction yield of approximately 10% (w/w). Least gelation concentration for JBPC was 20%. JBPC exhibited a predominantly hydrophobic nature, with good oil retention capacity and emulsion and foam stabilization properties. The structure of JBPC was more linear, stable and rigid, which primarily influenced gel stiffness. Based on the study of techno-functional properties, JBPC proved to be an excellent alternative to soy protein isolate and pea protein concentrate in various applications, with potential for becoming an innovative ingredient in the food industry. © 2025 Society of Chemical Industry.

Integrating Particle Motion Tracking into Thermal Gel Electrophoresis for Label-Free Sugar Sensing.

Bioanalytical sensors are adept at quantifying target analytes from complex sample matrices with high sensitivity, but their multiplexing capacity is limited. Conversely, analytical separations afford great multiplexing capacity but typically require analyte labeling to increase sensitivity. Here, we report the development of a separation-based sensor to sensitively quantify unlabeled polysaccharides using particle motion tracking within a microfluidic electrophoresis platform. Carboxymethyl dextran (20 kDa) was spiked into Pluronic thermal gel along with fluorescent nanoparticles (200 nm diameter) and loaded into single-channel microfluidic devices. Upon voltage application, the soluble sugar enriched into a concentrated band that induced motion of the insoluble particles as it passed. Bead displacement was tracked over time to produce electropherograms where peak areas were proportional to analyte concentrations. Key studies herein established the range of acceptable operating conditions (e.g., gel concentration, temperature) to characterize how the temperature-dependent rigidity of thermal gel influenced the analysis. Data processing strategies were then evaluated to identify conditions (e.g., exposure intervals, particle averaging, motion directionality) to maximize sensitivity. The quantitative response of the method was evaluated over a broad concentration range (0.5-5000 nM) where detection limits were found to be 520 pM for the 20 kDa sugar, providing a 106-fold superior mass LOD than a gold standard UV-vis absorbance method. Studies into the detection mechanism found that sensitivity was dependent on the molecular weight of the sugar as larger sugars produced greater responses. Collectively, these studies established best practices for integrating particle sensing into thermal gel separations for label-free polysaccharide quantitation.

Mesoscale modelling of fibrin clots: the interplay between rheology and microstructure at the gel point.

This study presents a numerical model for incipient fibrin-clot formation that captures characteristic rheological and microstructural features of the clot at the gel point. Using a mesoscale-clustering framework, we evaluate the effect of gel concentration or gel volume fraction and branching on the fractal dimension, the gel time, and the viscoelastic properties of the clots. We show that variations in the gel concentration of our model can reproduce the effect of thrombin in the formation of fibrin clots. In particular, the model reproduces the fractal dimension's dependency on gel concentration and the trends in elasticity and gelation time with varying thrombin concentrations. This approach allows us to accurately recreate the gelation point of fibrin-thrombin gels, highlighting the intricate process of fibrin polymerization and gel network formation. This is critical for applications in the clinical and bioengineering fields where precise control over the gelation process is required.

Fruit quality retention and shelf-life extension of papaya through organic coating.

Papaya (Carica papaya L.) is a climacteric fruit which lose quality and shelf life quickly due to physiological decay and microbial infection after harvest. The study was conducted to evaluate newly applied clybio formulation (0.2%) along with the existing effective concentration of chitosan (1%), aloevera gel (50%), seaweed extract (1%) and uncoated papaya (control) fruits on post-harvest physicochemical properties and disease incidence when stored at 25±1°C and 85-90% relative humidity. Quality parameters were available up to 12 days of storage (DAS) for chitosan and clybio treated papaya where it was 9 DAS for aloevera gel and seaweed treated papaya and 6 DAS for control papaya. Before decay of all the coated papaya at 9 DAS, chitosan (1%) performed superior in retaining maximum reducing sugar (0.77%, 1.41% and 3.85% more than aloevera, seaweed and clybio application, respectively), β-carotene (10.94%, 12.5% and 9.89% greater than aloevera, seaweed and clybio coatings, respectively), total flavonoids content (18.36%, 29.81% and 25.29% better than aloevera, seaweed and clybio treatments, respectively), total antioxidant activity (21.85%, 68.2% and 47.91% than noted in aloevera, seaweed and clybio formulations, respectively) and potassium content (3.14% and 9.32% than aloevera and clybio treatments, respectively). In addition, clybio gave better results over chitosan up to completion of shelf life (12 DAS) such as retention of ascorbic acid (6.21%), non-reducing sugar (13.48%), magnesium content (8.31%) and disease incidence (20%). Thus, besides preserving nutraceutical property, chitosan and clybio coated papaya remained edible for further 6 days compared to control, and 3 days over aloevera gel and seaweed extract treatment. These findings suggest the use of chitosan and clybio formulation for preserving quality parameters and extending the storage life of papaya.

Stiff-Stilbene-Linked Bis-Cholesterol: Synthesis and Investigation of Its Supramolecular Gelation and Photophysical Behaviors.

Here, we report the design, synthesis, and comprehensive characterization of the bis-cholesterol supramolecular gelator, which contains photochromic stiff-stilbene as a bridging unit. The cis-isomer of stiff-stilbene bridged bis-cholesterol (Z-D) was first synthesized with a systematic design, which can be further converted into its trans-isomer (E-D) with a high degree of efficiency (ca. 100%) upon exposure to 385 nm UV light. Unusual gelation behavior was observed for Z-D, which exhibited supergelator properties in mixed solvents of acetonitrile (ACN)/dichloromethane (DCM) (v/v = 1:1), DCM/MeOH (v/v = 1:2), ACN/CHCl3 (v/v = 2:1), and CHCl3/MeOH (v/v = 1:2), with minimum gelation concentrations (MGCs) as low as 0.2 w/v%. These gels formed rapidly at room temperature without the aid of any mechanical forces upon the addition of an antisolvent into the vial containing the gelator and its dissolving solvent. The formation of the self-assembled gel was primarily driven by hydrogen bonding, van der Waals forces, and dipole-dipole interactions, as confirmed by 1H NMR, Fourier transform infrared spectroscopy (FT-IR), and UV-vis spectroscopies. The gelator molecule Z-D entraps organic solvents and organizes itself into three-dimensional (3D) fibrillar networks in various single and mixed solvents, as confirmed by scanning electron microscopy (SEM) analysis. Upon irradiation with 385 nm light, the gel networks disintegrated into a precipitate suspension, resulting in the transformation of the fibrous structures into irregular spherical-like aggregates. This proves that the structural conformation changes in the gelator significantly influence the resulting self-assembled structures. Overall, the findings present in this study pave the way for the future development of novel light-responsive bis-cholesterol-based gelators, especially in their Z-isomeric form.

THE EFFECT OF CONCENTRATION AND SOAKING LENGTH OF ALOE VERA GEL ON THE GROWTH OF ROBUSTA COFFEE (Coffea canephora) CUTTINGS

This study aims to determine the effect of concentration and soaking time of aloe vera gel on the growth of robusta coffee cuttings. The research was arranged in a Randomized Complete Group Design (RAKL) with a factorial experiment (4x2) consisting of two treatment factors and repeated three times as a block. The first factor is the concentration of aloe vera gel with levels of 0, 20, 40, and 60%. The second factor is the length of soaking with levels of 5 and 10 hours. The results were analyzed using variance analysis, treatments that had a significant effect were further tested with Orthogonal Polynomial for the first factor and interaction and the Least Significant Difference (LSD) test for the second factor. The results showed that 40% aloe vera gel concentration produced the highest root dry weight. The treatment of aloe vera soaking time for 5 hours produced the longest root length. The interaction between aloe vera gel concentration of 20% and soaking time for 5 hours produces the longest root length.

Antibiofilm, Anti-Inflammatory, and Regenerative Properties of a New Stable Ozone-Gel Formulation.

Background/Objectives: Chronic skin wounds are characterized by inflammation, persistent infections, and tissue necrosis. The presence of bacterial biofilms prolongs the inflammatory response and delays healing. Ozone is a potent antimicrobial molecule, and many formulations have been used in the advanced therapeutic treatment of chronic wounds. The aim of this work was to determine the antimicrobial, anti-inflammatory, and regenerative activity of a stable ozone-gel formulation over time. Methods: The antimicrobial property was assessed by measuring the minimal inhibitory concentration and the antibiofilm activity. The anti-inflammatory effect was evaluated by TNF-α determination, and the regenerative effect was measured by scratch assay. Results: The ozone gel demonstrated antimicrobial and antibiofilm activity in all ATCC microorganisms examined and on most clinical isolates. Higher concentrations of the ozone gel were also useful in the dispersion of preformed biofilm. The ozone gel also showed anti-inflammatory activity by reducing the production of TNF-α and regenerative activity in human fibroblasts and keratinocytes. Conclusions: Given all these antimicrobial, anti-inflammatory, and regenerative characteristics, the ozone gel could be, in this formulation, used in the treatment of wounds. The ozone-gel formulation described here retains stability for over 30 months, which facilitates its use compared to formulations that lose efficacy quickly.

Boosting Supramolecular Gelation Efficiency and Properties: Ionic Strength as a Key to Superior Hydrogels.

Controlling the minimum gelation concentration (MGC) of low molecular weight (LMW) hydrogelators is a key for modulating gel properties, such as mechanical strength, viscoelasticity, and stability, which are crucial for applications ranging from drug delivery to tissue engineering. However, tweaking the MGC under specific conditions, such as pH and/or temperature, poses a considerable challenge. Herein, we varied the ionic strength of buffer solutions using NaCl for several LMW hydrogelators, including Fmoc-Phe, Fmoc-Tyr, Fmoc-Trp, Fmoc-Met, and Fmoc-Cha, and assessed their gelation efficiency at pH 7.4 and ambient temperature. Interestingly, Fmoc-Phe demonstrated a ∼67% (3-fold) MGC reduction, from 0.24 to 0.08 wt %, at 500 mM NaCl, transforming it a "super hydrogelator" (MGC < 0.1 wt %), while Fmoc-Trp showed 60% MGC reduction. Higher ionic strength effectively shields the electrostatic repulsion between negatively charged (-COO-) groups on the Fmoc-Phe, promoting closer aggregation and more efficient self-assembly and allowing for gelation at lower concentrations. In contrast, Fmoc-Met, Fmoc-Cha, and Fmoc-Tyr precipitated in the presence of NaCl, suggesting that NaCl specifically modulates the MGC of Fmoc-amino acid gelators containing unsubstituted aromatic side chains. Furthermore, these results indicate that cation-π interactions likely play a role, alongside carboxylic acid neutralization. While Fmoc-Phe forms gels in the presence of other monovalent cations, it does not form a hydrogel in the presence of divalent (CaCl2) and trivalent (AlCl3), indicating that enhancement of hydrogelation is specific to monovalent cations. Although the fibrillar structure of Fmoc-Phe hydrogels remained consistent, addition of NaCl increased fibril stickiness, creating densely packed networks that modulate the mechanical strength. Unlike typical cases where increased ionic strength leads to precipitation, Fmoc-Phe gelation at high NaCl concentrations (150-500 mM) is significant, yielding a robust supramolecular hydrogel that remains stable in high ionic-strength environments. This outcome suggests that ionic strength could be a valuable factor to enhance the efficient gelation of LMW hydrogelators.

Transport and Plugging Performance Evaluation of a Novel Recrosslinkable Microgel Used for Conformance Control in Mature Oil Fields with Superpermeable Channels

Summary Although recrosslinkable preformed particle gel (RPPG) has been successfully evaluated in the literature for mitigating conformance problems in open-fracture applications, there are no studies that have examined the transportation and plugging performance of microsized RPPGs (micro-RPPG) in high-permeability channels. We systematically evaluated a novel micro-RPPG and its transportation mechanism in matrix-like media. The micro-RPPG can recrosslink to form a bulk gel after being placed in the reservoir, combining the advantages of both in situ and preformed particle gel (PPG) systems. This recrosslinking ability prevents the gel from being washed out easily, ensuring sustained performance under reservoir conditions. In this study, the micro-RPPG characterization, self-healing process, transportation behavior, and plugging performance were investigated. A sandpack model with multipressure taps was utilized to assess the micro-RPPG suspension’s transport behavior and plugging efficiency. In addition, micro-optical visualization of the gel particles was deployed to study the particle size changes before and after the swelling process. Bottle tests showed that micro-RPPG could be dispersed and remain as separate particles in water with a concentration below 8,000 ppm, which is a favorable concentration for particle gel pumping. However, during the flooding test, the amount of micro-RPPG can be entrapped in the sandpack, resulting in a higher microgel concentration (higher than 8,000 ppm), endowing the gel particles with recrosslinking ability even with excessive water. The micro-RPPG could propagate through the sandpack model, and the required pressure gradient mainly depends on the average particle/pore ratio and gel concentration. The gel suspension significantly reduced channel permeability, providing sufficient resistance to post-waterflooding (more than 99.97% permeability reduction). In addition, the evaluation of micro-RPPG retention revealed that it is primarily affected by both gel concentration particle/pore ratios. We have demonstrated that the novel recrosslinkable micro-RPPG can transport through large channels, and it can also provide effective plugging due to its unique recrosslinking property. However, by this property, the new microgel exhibits enhanced stability and demonstrates resistance to being flushed out in such high-permeability environments. Furthermore, with the help of novel technology, it is possible to overcome the inherited problems commonly associated with in-situ gel treatments, including chromatographic issues, low-quality control, and shearing degradation.

Physicochemical, rheological, sensory properties and shelf life of processed cheese analogue prepared with oleogel sesame oil and mono- and di-glyceride (E471).

Analog processed cheeses are cheese-like products with diverse compositions and functional properties that are produced by replacing milk components with non-milk components. The aim of the study was to investigate physicochemical, rheological and sensory properties of cheeses and oleogels. The results showed; that increasing the concentration of Mono and di-glyceride increased the oil binding capacity and improved the characteristics of the oleogel texture and stable during storage, especially in terms of oxidative. The concentration of gelator, with attention to increasing the storage modulus (G') and the loss modulus (G'') important role in formation of the network structure. The use of oleogel in the processed cheese analogue formulation increased the oil binding capacity of the samples and decreased the meltability of the samples. The storage time in the refrigerator temperature did not have a significant effect on the measurement parameters. The samples of cheeses prepared with oleogel were more stable in terms of physicochemical than the control sample.

A physical crosslinked pH-sensitive hydrogel based on hemicellulose/graphene oxide for controlled oral drug delivery.

The design of innovative pH-sensitive hydrogels for oral drug delivery is particularly promising for the treatment of intestinal diseases. The traditional pH-responsive hydrogels still have some problems such as low biocompatibility, complex preparation process and poor therapeutic effect, so a new method needs to be developed to solve these problems. Here, a pH-sensitive hemicellulose/graphene oxide (HC/GO) composite hydrogel (HGCH) was prepared through a one-step strategy. Benefitting from the multiple hydrogen bonding between HC and GO, HGCH possessed a low gelator concentration (∼0.79wt%), well-defined 3D porous network and excellent mechanical properties. Remarkably, HGCH exhibited pH-induced gel-sol transition and a high drug loading efficiency, showing great potential as a candidate for advanced drug carrier. The drug loading and release test revealed that about 85% Vitamin B 12 was released in neutral PBS solution (pH7.4). However, only about 30% drug was diffused into acid medium (pH1.7) in the same period, which suggested the HGCH have high adaptability to soluble drugs and pH sensitivity triggered release. Further cellular toxicity tests demonstrated that the HGCH was nontoxic and biocompatible for cells. Thus, the physically cross-linked HGCH would be an attractive drug carrier for controlled drug release at physiological pH in the future.

Effects of Different Non-Ionic Polysaccharides on the Heat-Induced Gelling Properties of Curdlan.

Curdlan's application is constrained by high gelation concentration, poor water solubility, and incompatibility with other polysaccharides. To address these limitations, this study investigated the effects of different concentrations (0.05-0.3%) of non-ionic polysaccharides (pullulan (PL), locust bean gum (LBG), guar gum (GG), and konjac gum (KGM)) on the heat-induced gelling properties of curdlan. PL with no branch showed 0.3% enhanced gel hardness. LBG with a small amount of galactose residue and KGM with an acetyl group had similar effects on hardness, while GG with a large amount of galactose residue slightly weakened the mixed gel. The rheological results showed that PL had little effect on curdlan, and LBG and KGM had a positive effect on curdlan unfolding, but 0.3% GG was significantly antagonistic to curdlan. The above results implied that non-ionic polysaccharides without side chains interacted weakly with the curdlan and hardly changed the properties of curdlan. Curdlan unfolding and stable suspension were favored if the structure contained galactose or acetyl side chains that interacted with curdlan through hydrogen bonding. These results suggested an effective way to modify curdlan by strengthening the interaction of curdlan with others and weakening the hydrogen bonding of curdlan to broaden its application in food colloids.

α-D Nucleoside Based Self-Healing Supramolecular Hydrogels Derived from the α-D Anomers of 2'-Deoxyguanosine and Fluorescent 8-Azapurine 2'-Deoxyribofuranosides.

Self-assembly of α-D nucleosides to supramolecular hydrogels is described in detail. Hydrogel formation is studied on α-D 2'-deoxyguanosine (α-dG), and the fluorescent 8-azapurine α-D nucleosides 2-amino-8-aza-2'-deoxyadenosine (α-2-NH2-z8Ad) and 8-aza-2'-deoxyisoguanosine (α-z8iGd). These compounds were prepared from α-D 8-aza-2'-deoxyguanosine by an activation/amination protocol followed by deamination. Protonation and deprotonation pKa values of monomeric nucleosides were determined. Fluorescence measurements displayed the pH-dependent fluorescence intensity of α-D 8-azapurine nucleosides. α-dG and α-z8iGd self-assemble to gels that are selective for K+-ions. The α-dG gel is transparent and the α-z8iGd gel shows fluorescence. α-2-NH2-z8Ad forms fluorescent gels in the presence of alkali metal ions of different size. SEM images expose a large condensed and flat structure for the α-dG gel, whereas the α-2-NH2-z8Ad gel consists of flakes that are connected to bundles. A porous structure generated by helical cylindric fibers was found for the α-z8iGd gel. All α-D hydrogels show long-term lifetime stability. The α-z8iGd hydrogel in KCl solution has the highest Tgel value. The minimum gelation concentration of the hydrogels is 0.3-0.5 mg nucleoside/100 μL alkali ion solution. In periodical step-strain experiments, the hydrogels of α-dG, α-2-NH2-z8Ad and α-z8iGd displayed thixotropy. Based on their self-healing and shear-thinning properties the hydrogels are injectable.

Experimental Evaluation of a Recrosslinkable CO2-Resistant Micro-Sized Preformed Particle Gel for CO2 Sweep Efficiency Improvement in Reservoirs with Super-K Channels.

A recrosslinkable CO2-resistant branched preformed particle gel (CO2-BRPPG) was developed for controlling CO2 injection conformance, particularly in reservoirs with super-permeable channels. Previous work focused on a millimeter-sized CO2-BRPPG in open fractures, but its performance in high-permeability channels with pore throat networks remained unexplored. This study used a sandpack model to evaluate a micro-sized CO2-BRPPG under varying conditions of salinity, gel concentration, and pH. At ambient conditions, the equilibrium swelling ratio (ESR) of the gel reached 76 times its original size. This ratio decreased with increasing salinity but remained stable at low pH values, demonstrating the gel's resilience in acidic environments. Rheological tests revealed shear-thinning behavior, with gel strength improving as salinity increased (the storage modulus rose from 113 Pa in 1% NaCl to 145 Pa in 10% NaCl). Injectivity tests showed that lower gel concentrations reduced the injection pressure, offering flexibility in deep injection treatments. Gels with higher swelling ratios had lower injection pressures due to increased strength and reduced deformability. The gel maintained stable plugging performance during two water-alternating-CO2 cycles, but a decline was observed in the third cycle. It also demonstrated a high CO2 breakthrough pressure of 177 psi in high salinity conditions (10% NaCl). The permeability reduction for water and CO2 was influenced by gel concentration and salinity, with higher salinity increasing the permeability reduction and higher gel concentrations decreasing it. These findings underscore the effectiveness of the CO2-BRPPG in improving CO2 sweep efficiency and managing CO2 sequestration in reservoirs with high permeability.

Translating Solid-Phase Conformational Memory in the Prophecy of Multi-stimuli Responsive Low Molecular Weight Gels.

Polymorphism and its screening to select the best-performing form is in high demand. In low molecular weight organogels (LMWG), gelators are designed as they contain flexible groups, functionalities capable of varied H-bonding, and increased the potential to show polymorphism. We synthesized a bis-urea based LMWG G1 and isolated three distinct polymorphic phases (Form I, II, and III). G1 polymorphs showed noticeable differences in solubility; precisely, Form I is highly soluble compared to the other two. Gel screening was carried out for all three polymorphs using different stimuli like heat-cool, sonication, shaking, and grinding. Among the polymorphs, Form I was found to have better gelling ability which was reflected by the solvent scope, thermal stability (gel-sol transition temperature Tgel), minimum gelator concentration (M.G.C.), stimuli-responsiveness, morphology, and rheological properties. The differences in their gelation performance among the three polymorphs are associated with their solubility parameter. Stimuli like sonication, shaking, and grinding triggered Form I to form a gel. Form II and III responded to heat-cool stimuli only due to poor solubility. Therefore, it is noted crucial to add polymorph screening as an integral part of the gel synthesis to avoid problems associated with reproducibility in the gel prophecy of LMWG systems.

Enhancing stability and bioactivity of Chondrus ocellatus polyphenols through nanoparticle fabrication

This article aimed to fabricate Chondrus ocellatus polyphenols (COPs)-gelatin-chitosan nanoparticles to enhance their stability and bioactivity. Different preparation conditions were tested to investigate the effects of formulation on nanoparticle fabrication. Free radical scavenging activity of COPs and their nanoparticles were compared. The consequences revealed that optimal preparation was obtained with a chitosan (CS) concentration of 0.5 mg/mL, gelatin (Gel) concentration of 1.0 mg/mL, COPs concentration of 5.0 mg/mL, and Gel-CS-COPs mass ratio of 2:1:1. The resultant nanoparticles had the particle size of 39.79 ± 5.15 nm and encapsulation efficiency of 60.95 % ± 1.86 %. The COPs-Gel-CS nanoparticles were distributed uniformly, and no obvious aggregation was observed by transmission electron microscopy. Nanoencapsulation of the COPs significantly improved their antioxidative stability. This study provided a potential formulation for the application of Chondrus ocellatus polyphenols in antioxidant activities.

A Natural Carbon Encapsulated in Gellan-Based Hydrogel Particles Designed for Environmental Challenges.

This article reports the obtention of a new gellan-based hydrogel linked with Fe3+ and loaded with a natural micro/nanostructured carbon designed as a contaminant's removal from wastewater. Hydrogels are known for their water-retaining properties, high binding capacity, and eco-friendly features. The new material is expected to behave as one cost-effective and efficient sorbent, including natural carbon structures with various functional groups. The encapsulation efficiency ranges between 89% and 95%. The obtained hydrogel particles were characterized using FT-IR spectroscopy and scanning electron microscopy techniques. The hydrogel particles' water stability was evaluated by measuring the transmittance for 10 days, and the capacity to retain water was assessed by determining the swelling degree (Q%). The results showed that hydrogel particles are stable (the transmittance value is higher than 97.8% after 10 days), and their properties are influenced by the cross-linking degree, the amount of the carbon particles encapsulated, and the concentration of gellan. For example, the Q% values and encapsulation efficiency increased when the cross-linking degree, the carbon microstructure quantity, and the gellan concentration decreased. The new hybrid material can retain Pb(II) ions and diclofenac molecules, and could be used in different adsorption-desorption cycles.

In vitro cytotoxicity assessment of carbonaceous gels for bone marrow mesenchymal stem cells

The current work aimed to elucidate the potential applications of the carbonaceous gels and assess the in vitro cytotoxicity of these gels when suspended in a culture medium and exposed to bone marrow mesenchymal stem cells. Cellular viability, cell cycle distribution, apoptotic cell death, and mitochondrial membrane potential in bone marrow mesenchymal stem cells co-incubated with different concentrations of carbonaceous gels (0.1, 1, 10, 50, and 100μg/mL) were evaluated. Flow cytometry and immunofluorescence were used to investigate apoptosis and cell cycle distribution. The expression of associated apoptotic proteins was analysed using Western Blot. Although the co-incubation of carbonaceous gels did not significantly affect cell viability, high dosages (100μg/mL) of these gels led to cellular dysfunction. Specifically, cells exposed to high concentrations of these gels exhibited G2-phase arrest and increased levels of reactive oxygen species. However, the reported impacts did not cause considerable cell death. At the same time, carbonaceous gels did not significantly induce apoptosis. Compared to other carbon nanomaterials, carbonaceous gels' biotoxicity was relatively low, suggesting their potential for various biological applications. Nonetheless, caution should be exercised when considering the concentration of carbonaceous gels for future medical applications.