Concentrations Of Ethylenediaminetetraacetic Acid Research Articles

Effectiveness of Root Canal Irrigation and Dressing for the Treatment of Asymptomatic Apical Periodontitis: A Systematic Review Assessing Complementary Timeframes.

Sodium hypochlorite (NaOCl), ethylenediaminetetraacetic acid (EDTA), and calcium hydroxide (Ca(OH)2) are used in root canal treatment. However, further research is needed to assess their effectiveness at time points not considered in previous guidelines. This review complements the systematic review by Rossi-Fedele and Rödig (2023) by considering time points excluded by the latter. A search was conducted in PubMed-MEDLINE, Scopus, EMBASE, Google Scholar, and repositories between March and May 2024. Four studies met the inclusion criteria, providing data from 212 teeth for irrigation and 108 for dressings. No significant differences in pain reduction within 7 days or radiographic reduction of apical lesion size were found between 2% chlorhexidine, 5.25% NaOCl, EDTA, and various NaOCl concentrations. Single and multiple visit protocols also showed no significant differences in postoperative pain.Two studies had a 'low risk' of bias and two 'some concerns'. Evidence suggests that Ca(OH)2 dressings may not offer additional benefits.

Study on the enhancement effect of EDTA and oxalic acid on phytoremediation of Cr(VI) from soil using Datura stramonium L.

This study investigated the enhancing effects of soil treatment with ethylene diamine tetraacetic acid (EDTA) and oxalic acid (OA) on the remediation of Cr(VI) contaminated soil by Datura stramonium L. A greenhouse pot experiment was conducted, where Cr(VI) contaminated soil was treated with 100 mg/kg Cr(VI) and varying concentrations of EDTA (5 and 10 mmol/kg) and OA (5 and 10 mmol/kg). The effects of these soil treatments on biomass, chlorophyll content, antioxidant enzyme activities, and Cr(VI) enrichment and translocation efficiency of D. stramonium were evaluated. The results showed that added OA to soil significantly increased the biomass and chlorophyll content of D. stramonium. The addition of 10 mmol/kg of OA to soil increased the plant biomass by 67.16 % and chlorophyll b content by 40.01 %. In addition, OA soil treatment significantly enhanced the activities of superoxide dismutase (SOD) by 6.36 %, peroxidase (POD) by 163.13 %, catalase (CAT) by 36.92 %, and ascorbate peroxidase (APX) by 32.12 %, which effectively alleviated the oxidative stress induced by Cr(VI). In contrast, soil treatment with a high concentration of EDTA (10 mmol/kg) significantly reduced plant biomass and chlorophyll content, although it increased the biological concentration factor (BCF) of the stem and leaf, as well as the translocation factor (TF). In conclusion, appropriate amounts of EDTA and OA added to soil can enhance the phytoremediation efficiency of D. stramonium grown in Cr(VI) contaminated soil, with OA added to soil being more effective than addition of EDTA. This study revealed the potential mechanisms of chelating agents EDTA and OA in enhancing the phytoremediation of Cr(VI) contaminated soil by D. stramonium, providing a scientific basis for further optimization of phytoremediation techniques.

The impact of calcium sequestration on the material and structural properties of acid milk gels and corresponding cream cheeses

SummaryWe report on the role of ethylenediaminetetraacetic acid (EDTA) in addition to the structural and material properties of acid milk gels formed via addition of glucono‐delta‐lactone (GDL) and these properties of corresponding analogue cream cheeses prepared from these gels. Increasing addition of EDTA to cheese milks prior to acidulation reduced the concentration of insoluble calcium in the milks. Acid gelation of milks, as measured by small deformation rheology, showed a decreased rate of gelation and lower relative elastic modulus of gels with increasing EDTA concentration. The sequestration of colloidal calcium phosphate from the casein micelle by the addition of EDTA led to the lower gelation rate and elastic modulus. The lower extent of ionic binding is considered the causative mechanism for formation of weaker gel structures. Cream cheeses prepared using acid gels with GDL acidulant were compositionally unaffected by EDTA concentration. However, variations in G′ observed in the acid gels containing increasing levels of EDTA correlated with large deformation material property changes in cream cheese analogues. In addition, the increasing inclusion of EDTA in cheese milks translated to reduced water‐holding capacity of the cream cheeses prepared from those milks. These results provide insights on the specific role of milk calcium on the structure, material and physical properties of acid milk gels and corresponding cream cheese analogues.

Vermifiltration for removal of emerging contaminant EDTA (ethylenediaminetetraacetic acid). Kinetics models for adsorption, bio- and vermidegradation

Vermifiltration is a sustainable technology that combines the action of microorganisms and earthworms to remove contaminants in wastewater. In this work, vermifiltration was used to remove the emerging contaminant, EDTA, from simulated greywater. Three types of filters were designed: Azide Filter (AF) to evaluate the adsorption process, Bio filter (BF), to evaluate adsorption plus the action of the microorganisms, and Vermi Filter (VF), to assess the previously described processes and the action of the earthworms. Each filter was filled with gravel, sand and an active layer, and VF was inoculated with 60 Eisenia fetida. The filters were operated on a batch system with recycling. Different regimes were observed: an initial rapid decrease of EDTA concentration by adsorption; a lag phase, which lasted from 7 to 18 days, and a final EDTA decrease due to biological or vermi degradation. A mathematical model was developed performing the EDTA mass balance in the system and considering a first order kinetic rate for EDTA removal. Experimental data was fitted by the model, determining kinetic constants for adsorption or degradation process, kads and kd respectively. The kads values were similar for all filters, whereas VF showed a kd 53 % higher than that of BF and AF. Earthworms survival was 85 % and, although the biomass was significantly reduced (64.8 %), there was cocoons and juveniles. Bioassays with Lactuca sativa showed no phytotoxicity of the treated wastewaters. All the systems were efficient but the VF was faster, supporting that vermifiltration is a suitable technology for treating EDTA greywater.

Enhanced oil recovery and mitigating challenges in sandstone oil reservoirs employing EDTA chelating agent/ seawater flooding

While low salinity water (LSW) holds promise for improving oil recovery, its implementation is comparatively costly due to the substantial volumes of fresh water required to dilute seawater (SW). Additionally, concerns arise regarding potential issues related to LSW, including fines migration in sandstone reservoirs and scale formation in carbonate reservoirs. Chelating agents can be introduced into undiluted SW to bind with metal ions, thereby lowering its salinity. This approach mimics the behavior of LSW injection while overcoming associated challenges. This study involves measuring oil/injected solution interfacial tension (IFT), evaluating sandstone surface wettability alteration, along with conducting different sand pack flooding scenarios using Ethylenediaminetetraacetic acid (EDTA) chelating agent. The aim was to comprehend the EDTA oil recovery mechanisms and determine the optimal concentration of EDTA that minimizes chemical consumption while maximizing oil recovery. The findings indicated that incorporating 5 wt% EDTA into SW significantly reduced IFT from 29.52 mN/m to 4.75 mN/m, making the oil nearly miscible in the solution. Moreover, wettability tests confirmed that a minimum EDTA concentration of 5 wt% is required to shift wettability from oil-wet to strongly water-wet conditions. Sand pack flooding experiments, on the other hand, further demonstrated that this optimized fluid system can potentially recover an additional 16.1 % of oil following SW flooding. Finally, inductively coupled plasma (ICP) analysis revealed a notable increase in metal ion concentration during EDTA flooding, indicating enhanced leaching of metals from the sandstone surface and improved wettability characteristics.

Effects of Ethylenediaminetetraacetic Acid and Some Chlorides of Divalent Metals on the Initial Velocity of Crude Peroxidase from Watermelon Rind

Aim: This study investigated the effects of ethylenediaminetetraacetic acid (EDTA) and some chlorides of divalent metals on the initial velocity of crude peroxidase from watermelon rind Study design: In vitro enzyme assay. Place and Duration of Study: Department of Biochemistry, Faculty of Life Sciences, Ambrose Alli University, Ekpoma, Edo State, Nigeria between September 2023 to November 2023. Methodology: The kinetics of the oxidation of Guaiacol by the crude peroxidase from the rind of watermelon in the presence of varying concentrations of EDTA and the chloride salts of iron, copper was determined spectrophotometrically by monitoring the oxidation of Guaiacol to produce a brown tetraguaiacol at a wavelength of 470nm. The various salt concentrations were varied between 0.5 mM and 3 mM. Each of the reaction mixtures used in the kinetic study comprised; 2.3 mL of 0.6 M sodium acetate buffer (pH 5.4), 0.2 mL of 0.02 mM Guaiacol, 0.1 mL of crude extract, 0.2 mL of varying concentration chloride salt, 0.2 mL of 2 mM of H2O2 added last to start the reaction. The final concentration of H2O2 in the 3 mL assay was 0.13 mM. The total volume of the reaction mixture was 3 mL. The absorbance was read every 2 seconds for one minute after adding hydrogen peroxide using a stop-clock. The control had no metal ion but replaced with distilled water Results: Results showed that EDTA reduced the activity of the enzyme in a concentration dependent manner. CuCl2 and FeCl2 activated the enzyme and FeCl2 being a better activator within the salt concentration range of 0.5 mM to 3 mM. Conclusion: These findings are of great importance to industries in understanding the mechanism of action of peroxidase from the rind of watermelon, especially as the search for cheap and alternative sources of peroxidases continues.

Removal of Metal from Carboxypeptidase A Proceeds via a Split Pathway: Implications for the General Mechanisms of Metalloenzyme Inactivation by Chelating Agents.

The chelation of protein-bound metal ions is typically thought to follow either a dissociative (D) or an associative (A) path. While the former mechanism involves the spontaneous dissociation of the metal from the protein prior to chelation, the latter route is characterized by the formation of an intermediate protein-metal-chelator ternary complex. Using the prototypical zinc protease carboxypeptidase A (CPA) and a variety of charged and neutral chelating agents, we demonstrate that inactivation of the enzyme (and likely other metalloproteins) proceeds through a split pathway with contributions from both D- and A-type mechanisms. In the case of charged chelators such as ethylenediaminetetraacetic acid (EDTA), the proportions of both paths could be tuned over a wide range through variation of the chelator concentration and the ionic strength, I (from ∼95% A type at low I values to ∼5% at high I values). By measuring the EDTA concentration and time dependence of CPA inactivation and fitting the obtained kinetic data to a modified time-dependent inhibition model, we obtained the rate constants for the A and D paths (kinact and koff, respectively) and the inhibition constant (KI) for the formation of the CPA-Zn2+-EDTA ternary complex, indicating that the decreased contribution of the A-type mechanism at high ionic strengths originates from a large (40-fold; at I = 0.5 M) increase in KI. This observation might be related to a triarginine motif in CPA that electrostatically steers negatively charged substrates into the active site and may therefore also guide carboxylate-bearing chelators toward the Zn2+ ion.

Tuning ZnSe nanostructures for enhanced ammonia sensing at room temperature

Nanostructured materials, defined by their distinctive physicochemical properties at the nanometer scale, facilitate the innovation of sophisticated applications through their inherent size-dependent phenomena and surface characteristics. In this study, we explore the synthesis of ZnSe nanostructures with distinct spherical and flower-like morphologies by varying the concentration of Ethylenediamine tetraacetic acid (EDTA). Our findings reveal that higher concentrations of EDTA facilitate flower-like morphologies, which provide a large surface area desired for gas sensing applications. Herein, the as-synthesized ZnSe nanostructures were applied for ammonia (NH3) sensing at room temperature at different concentrations to evaluate their performance. The results obtained indicate that ZnSe nanostructures with flower-like morphology exhibit superior sensing capabilities, demonstrating a good response (ΔR/Rair)% of 71 % compared to the 61 % response observed for spherical ZnSe nanostructures for 20 ppm NH3 gas, measured at room temperature. Additionally, the flower-like ZnSe nanostructures show significantly enhanced response and recovery times, demonstrating their potential as efficient materials for NH3 detection at room temperature.

Effect of EDTA with porous balloon on calcified lesion: An atherosclerotic cadaver study.

Ethylene diamine tetra-acetic acid (EDTA) is a chelating agent used to dissolve calcium deposits but evidence in decalcifying atherosclerotic lesions is limited. We assessed the feasibility and efficacy of EDTA delivered via porous balloon to target calcified lesions in cadaveric below-the-knee (BTK) arteries. Using porcine carotid arteries, EDTA concentration was measured in the arterial wall and outside the artery at the 0-, 0.5-, 4-, and 24-h circulation after the injection through a porous balloon. In cadaver BTK samples, the proximal and distal anterior tibial artery (ATA) and distal posterior tibial artery (PTA) were studied. EDTA-2Na/H2O or EDTA-3Na/H2O were administrated using a porous balloon, then circulated for 6 h for EDTA-3Na/H2O and 24 h for EDTA-2Na/H2O and EDTA-3Na/H2O. Micro-CT imaging of the artery segments before and after the circulation and cross-sectional analyses were performed to evaluate calcium burden. In the porcine carotid study, EDTA was delivered through a porous balloon present in the arterial wall and was retained there for 24 h. In BTK arteries, cross-sectional analyses of micro-CT revealed a significant decrease in the calcium area in the distal ATA segment under 24-h circulation with EDTA-2Na/H2O and in the distal ATA segment under 24-h circulation with EDTA-3Na/H2O. The proximal ATA segment under 6-h circulation with EDTA-3Na/H2O showed no significant change in any parameters of calcium CONCLUSION: EDTA-3Na/H2O or EDTA-2Na/H2O with longer circulation times resulted in greater calcium reduction in atherosclerotic lesion. EDTA may have a potential therapeutic option for the treatment of atherosclerotic calcified lesions.

Unraveling the Impact of Ethylenediaminetetraacetic Acid Chelating Agents on the Oil Recovery Processes: An Insight into Its Role in Fluid-Fluid and Rock-Fluid Interactions.

In recent years, chelating agents have garnered increasing attention due to their unique capabilities in addressing challenges associated with chemically enhanced oil recovery materials. Most previous studies have primarily focused on examining the influence of chelating agents on rock-fluid interactions. However, their effects on fluid-fluid interactions, specifically on interfacial tension (IFT) characteristics under various conditions, remain relatively unknown. This study investigates the effect of crucial reservoir parameters on the performance of ethylenediaminetetraacetic acid (EDTA) chelating agents in the IFT between crude oil and brine. Furthermore, the study evaluated the impact of varying concentrations of this agent on rock wettability alteration, zeta potential, and spontaneous imbibition. The results illustrated that introducing EDTA at high concentration into seawater (SW) solution reduced the IFT by 85%. Additionally, while higher pH levels contributed to IFT reduction due to increased hydroxyl ions, excessive salinity levels resulted in elevated IFT. Raising the temperature from 30 to 75 °C further decreased the IFT, changing the IFT for optimal EDTA concentration from 20.43 to 2.56 mN/m (∼88% reduction). The changes in zeta potential and contact angle measurements indicated that solutions of 5 and 7 wt % EDTA shifted rock wettability from oil-wet to strongly water-wet, resulting in the wettability alteration index of 1.01 and 1.02, respectively. Inductively coupled plasma analysis, on the other hand, revealed substantial chelation of metal ions from both the solution and rock when EDTA was added to the rock/SW system. This resulted in a significant increase in Ca2+ ions and a decrease in Mg2+ ions, attributed to the multi-ion exchange phenomenon.

Coupling interface constructions of ZnO@ZnS@FeOOH for photocatalytic hydrogen production performance

Photocatalysis is a reliable method to solve energy crisis and generate green energy hydrogen. ZnO@ZnS (ZOS) formed heterojunction repressed carrier complexation and thus enhanced photocatalytic performance, but its low photoresponse efficiency limited its photocatalytic performance. Therefore, in this paper, ZnO@ZnS was modified with ethylene diamine tetraacetic acid (EDTA) to form ZnO@ZnS@FeOOH composites with coupling interface. The modification of EDTA could inhibite the oxidation of FeOOH during the synthesis process, and what’s more it could enhance the charge transfer at the heterojunction interface to promote the separation of electron-hole pairs and photoresponsive ability to further enhanced the hydrogen precipitation performance. The concentration of EDTA and Fe sources were regulated to investigate the photocatalytic hydrogen production capacity of the composites. The results indicated that the ternary composite ZnO@ZnS@FeOOH had the superior hydrogen evolution performance with the hydrogen evolution rate of 529.17 umol g−1 h−1, which was 2.4 times higher than that of ZOS. EDTA acts as an electron channel to enhance the inter-interfacial electron transfer capability, and the loading of FeOOH formed a heterojunction structure, which promoted carrier separation and enhanced the photoresponse. It’s the synergistic effect to enhanced the photocatalytic hydrogen production performance. This paper provided a method for the construction of ternary heterojunctions and interfacial charge transport channels.

Polycarboxylate functionalized magnetic nanoparticles Fe3O4@SiO2@CS-COOH: Preparation, characterization, and immobilization of bovine serum albumin

Magnetic nanoparticles with increasing superparamagnetism and magnetic targeting have found widespread application in fields such as food and medicine. In this study, polycarboxylated magnetic nanoparticles (Fe3O4@SiO2@CS-COOH) were prepared by surface functionalizing iron tetraoxide (Fe3O4) nanoparticles with ethylenediaminetetraacetic acid (EDTA) as a modifier. The appropriate degree of functionalization modification was obtained by adjusting the EDTA concentration and the ratio of cross-linking agents. The prepared magnetic nanoparticles were analyzed with structural and property characterization. The results showed that the Fe3O4@SiO2@CS-COOH magnetic nanoparticles prepared with 4 % EDTA and cross-linking agents at a molar ratio of 3:4 were uniform in particle size, with an average size of roughly 7 nm, and possessed an abundant carboxylate content (310.8064 μmol/g) and a high magnetization intensity (35.05 emu/g). As a model protein, bovine serum albumin (BSA) was immobilized on the surface of magnetic particles. The largest amount of immobilized protein was 500.4376 mg BSA/g at pH 4.0 and no extra salt ions. According to molecular docking simulations, its immobilization was due to the interaction of amino and carboxyl groups at the Fe3O4@SiO2@CS-COOH/BSA interface. Fe3O4@SiO2@CS-COOH possesses a large number of carboxyl groups, strong protein immobilization, and magnetic responsiveness, which may have potential applications in biomedical and food fields.

Regulating the distribution of phosphorus in sewage sludge hydrothermal carbonization products by complexation pretreatment

In this study, we investigated the effects of the concentrations of four pretreatment complexing reagents, namely ascorbic acid (AS), citric acid (CA), N, N-bis (carboxymethyl) glutamic acid (GLDA), and ethylenediaminetetraacetic acid (EDTA), on the phosphorus contents and forms in hydrothermally carbonized sludge products. The results showed that among the four complexing reagents, EDTA had the greatest effect on the phosphorus transfer from the solid phase to the liquid phase during the hydrothermal process of the sludge, followed, respectively, by GLDA, CA, and AS. The phosphorus proportion of the liquid phase was 63.96% under hydrothermal process temperature, reaction time, and EDTA concentration of 160 °C, 60 min, and 0.2 mol/L, respectively. The results showed formations of EDTA complexes with Ca, Fe, and Al in the sludge, releasing phosphorus bound to the metal ions into the liquid phase. The highest total phosphorus concentration in the liquid phase was 2533.31 mg/L following the increase in the hydrothermal process temperature from 100 to 160 °C. However, the Fe and Al-EDTA complexes were thermally decomposed after continuously increasing the hydrothermal process temperature to 240 °C, resulting in a readsorption of phosphorus to the solid phase with Fe, Al, and other metals and, consequently, decreasing the phosphorus content to 880.93 mg/L in the liquid phase. The results of this study provide a scientific basis for regulating phosphorus distribution in the solid and liquid phases of hydrothermally carbonized sludge products and effectively recovering phosphorus in the liquid phase.

New insights into the effect of EDTA on pyrite oxidation and N2O emission during pyrite autotrophic denitrification

Pyrite autotrophic denitrification (PAD) is crucial for removing nitrate in groundwater and advanced wastewater treatment. While disulfide is typically seen as the electron donor, the role of iron in pyrite oxidation is often ignored. In this study, Ethylene Diamine Tetraacetic Acid (EDTA) had been proven to have the potential to facilitate pyrite oxidation by promoting the solubility of iron in solution, thereby enhancing the kinetics of pyrite autotrophic denitrification. Firstly, EDTA could improve PAD performance, i.e. the nitrate removal rate at EDTA addition of 5 mM was 0.69 mg N/L•h and twice that of the control. However, high concentrations of EDTA inhibited the activity of the nitrous oxide reductase, resulting in the maximum accumulation of N2O reaching 13.26 mg N/L with an EDTA concentration of 15 mM. Furthermore, the X-ray Photoelectron Spectroscopy (XPS) result indicated that the EDTA addition promoted the production of bioavailable sulfur, with the concentration of S0 at 15 mM EDTA being more than twice higher than that of the control. Transmission electron microscopy (TEM) analysis indicated that EDTA could relieve the damage of iron encrustation on cell activity because it was difficult for EDTA-chelated ferrous iron to penetrate the cell membrane. Microbial community analyses showed an increase abundance of iron oxidizing bacteria, suggesting that EDTA addition promoted the bioavailability of iron for Fe(II) autotrophic denitrification. Overall, this study will provide insights into the understanding of oxidation mechanism of PAD process and the practical application for efficient nitrogen removal and N2O mitigation.

Extraction of copper (II) ions from oxidized ore in the presence of an ethylenediaminetetraacetic acid solution in a basic environment

An experiment was conducted to obtain copper (II) ions in a basic environment using ethylenediaminetetraacetic acid (EDTA) and oxidized copper minerals from Cerro Azoguini in Puno, Peru. Specific objectives included investigating the solubility of Cu2+ ions in a mineral with high silica, limestone, and iron content, as well as studying the influence of pH, EDTA concentration, and extraction time. 1.5 grams of ground mineral at -100 mesh (150 µm), with 80% pass and 6% solids, were leached with a 0.5 M NaHCO3 solution at different pH levels and EDTA concentrations. The results indicated the extraction of 3.5 g/L of Cu2+ ions at pH 10 with 0.12 M EDTA, without agitation, at room temperature (19°C), over a period of 83 hours. It was observed that the NaHCO3 solution extracted copper from the mineral, and EDTA quickly chelated it, forming ionic complexes such as CuHEDTA ∧ (1−), CuHEDTA ∧ (2−), and CuHEDTA ∧ (3−). Other metals present in the gangue were not chelated by EDTA, demonstrating its high selectivity based on solubility constants and precipitation formation at different pH levels. Additionally, high copper extraction rates were obtained at a pH of 13.15, possibly due to decreased protons and increased ions from both the reagent and leached mineral. In conclusion, the physicochemical analyses revealed the feasibility of obtaining Cu2+ ions by solubilizing oxidized minerals, followed by rapid chelation with EDTA to form diverse metal complexes depending on the pH of the medium.

Pattern of Lead Accumulation in Two Vegetable Plants Due to EDTA Treatment

Phytoextraction and phytostabilization are the most consistent patterns or mechanisms of action of phytoremediation. One of the elements influencing the mechanism of action of heavy metal absorption by plant species is Ethylene Diamine Tetraacetic Acid (EDTA). Therefore, this study aimed to determine the pattern of phytoremediation in water spinach and spinach due to the addition of EDTA in the soil. The treatments tested by factor 1 were water spinach (T1) and spinach (T2), and factor 2 was the concentration of EDTA consisting of 3 levels, 0, 3, and 6 g/polybag. Each treatment was repeated three times on five sample plants. Furthermore, growth evaluation was carried out in the first six days after planting and conducted every 3 days. It was carried out on variables such as changes in plant height, leaves area, total root length, Pb content in the soil, fresh and dry weight of shoots and roots, shoot, seeds, and Translocation Factor (TF). The results showed that water spinach and spinach had different mechanisms of action due to the application of EDTA in Pb-contaminated media. Furthermore, water spinach and spinach have a mechanism of phytoextraction and phytostabilization, respectively. Therefore, spinach is safer than water spinach when grown in Pb-polluted land.

Inhibition and Reduction of Biofilm Production along with Their Antibiogram Pattern among Gram-Negative Clinical Isolates.

Bacterial biofilm is a significant virulence factor threatening patients, leading to chronic infections and economic burdens. Therefore, it is crucial to identify biofilm production, its inhibition, and reduction. In this study, we investigated biofilm production among Gram-negative isolates and assessed the inhibitory and reduction potential of ethylene diamine tetra acetic acid (EDTA) and dimethyl sulfoxide (DMSO) towards them. In addition, we studied the antimicrobial resistance pattern of the Gram-negative isolates. Bacterial isolation and identification was done using standard microbiological techniques, following the Clinical and Laboratory Standards Institute (CLSI) guideline, 28th edition. The Kirby-Bauer disk diffusion method was used to determine the antibiotic susceptibility pattern of the isolates, and β-lactamase production was tested via the combination disk method. Biofilm formation was detected through the tissue culture plate (TCP) method. Different concentrations of EDTA and DMSO were used to determine their inhibitory and reduction properties against the biofilm. Both inhibition and reduction by the various concentrations of EDTA and DMSO were analyzed using paired t-tests. Among the 110 clinical isolates, 61.8% (68) were found to be multidrug resistant (MDR). 30% (33/110) of the isolates were extended-spectrum β-lactamase (ESBL) producers, 14.5% (16/110) were metallo-β-lactamase (MBL), and 8% (9/110) were Klebsiella pneumoniae carbapenemase (KPC) producers. Biofilm formation was detected in 35.4% of the isolates. Biofilm-producing organisms showed the highest resistance to antibiotics such as cephalosporins, chloramphenicol, gentamicin, and carbapenem. The inhibition and reduction of biofilm were significantly lower (p < 0.05) for 1 mM of EDTA and 2% of DMSO. Isolates forming biofilm had a higher resistance rate and β-lactamase production compared to biofilm nonproducers. EDTA and DMSO were found to be potential antibiofilm agents. Hence, EDTA and DMSO might be an effective antibiofilm agent to control biofilm-associated infections.

Effects of EDTA on the leaching behaviors of Cs(I) and Co(II) from cement waste forms

Cementation is extensively employed for immobilizing radionuclides in low- and intermediate-level radioactive wastes generated during the decommissioning of nuclear power plants. Ethylenediaminetetraacetic acid (EDTA), used as a decontamination agent during the decommissioning process, can be introduced into the cement waste form containing radionuclides. This study investigated the effects of the EDTA present in simulated radioactive decommissioning wastes on the leaching behaviors of Co and Cs immobilized in the cement waste form. Co leaching was facilitated by the formation of highly mobile Co–EDTA complexes. However, Cs leaching was impeded by the competition for leaching with other metal–EDTA complexes. Moreover, the EDTA-induced carbonated layer with a dense pore structure played a crucial role as a retardation barrier for the Cs leaching. The calcite contents of the samples with 8 wt% EDTA were approximately three times higher than those of the samples without EDTA. The introduced EDTA affected the leaching behaviors of both Co and Cs, as well as the microstructure evolution of the cement waste form. Nevertheless, the addition of EDTA had a relatively low positive effect on the efficiency of Cs immobilization, but also an obvious negative effect on the efficiency of Co immobilization, regardless of the concentration of EDTA. Finally, an EDTA dosage of 1 wt% in the cement waste forms containing Co or Cs is suggested as a potential waste acceptance criterion for solidified low- and intermediate-level radioactive waste.

Effects of ethylenediaminetetraacetic acid on growth and survival of long-spined sea urchin Diadema antillarum larvae

Abstract Objective The presence of heavy metals in seawater is a major challenge for hatchery culture of sea urchin larvae due to their sensitivity to metal pollution. The impact of heavy metals in seawater for sensitive marine larviculture could be minimized via chelation of metals using chemical compounds such as ethylenediaminetetraacetic acid (EDTA). Methods In this study the effects of EDTA were tested for the long-spined sea urchin Diadema antillarum during larviculture at two concentrations (10 μM and 50 μM) and two exposure durations (6 and 24 days postfertilization). Growth and survival of larvae were evaluated as response variables. Result Significant differences in growth and survival of larvae were found among the treatments at different EDTA concentrations and exposure durations. The 10-μM EDTA treatment enhanced growth and survival of larvae relative to control for both exposure durations. The 50-μM EDTA treatment was unsuitable for long-spined sea urchin larviculture due to reductions in growth and survival. Conclusion The use of 10 μM EDTA during embryo incubation and larviculture is expected to improve long-spined sea urchin larval fitness, leading to improved hatchery production.

Impact of ethylene diamine tetraacetic acid on physiochemical parameters and yield attribute in two varieties of Brassica juncea under lead stress.

Lead (Pb) is one of the most toxic elements on earth. The harmful effects of Pb at higher concentrations were seen on plant vegetation because plants are directly exposed towards it. Indian mustard, a well-known hyperaccumulator plant is the most promising crop for the environment, engaged in a variety of scenarios for ecological cleanup. In the present study, we used ethylene diamine tetraacetic acid (EDTA), a chelating agent that is of remarkable efficiency. The pot experiments were conducted in soil pretreated with 1000 mgkg-1 Pb with different concentrations of EDTA (2-10 mmol). All the growth parameters were reduced significantly in the plants treated with Pb and EDTA, however, a non-significant effect was observed in 5 mmol EDTA compared to Pb alone treatment. Photosynthetic pigments yield, nitrate reductase activity and NPK content were affected negatively; in contrast, superoxide dismutase and catalase activity was increased in Pb and Pb+EDTA treated in both the varieties. The Pb accumulation was elevated significantly by the augmentation of 5 mmol EDTA in both varieties. Accumulation of Pb in the shoot was higher in PM 25 than in P. Vijay, whereas root Pb accumulation showed the opposite, i.e., more Pb in roots of P. Vijay than PM 25. Moreover, The Pb accumulation per plant was observed more in P. Vijay as compared to PM 25. Hence, the present study implies that the augmentation of Pb-polluted soil with EDTA works well while dealing with B. juncea assisted phytoremediation and P. Vijay to be a stronger variety than PM 25. Further, 5 mmol of EDTA was optimum for phytoremediation of the soil polluted with up to 1000 mg Pb kg-1 soil.