Plasma Estradiol Concentrations Research Articles

Behavioral, Physiological and Hormonal Changes in Primiparous and Multiparous Goats and Their Kids During Peripartum

The objective of this study was to evaluate the effect of maternal experience on behavior, progesterone and estradiol concentrations, weight and body condition in goats in the peripartum period, as well as the behavior and weight of the kids in the first hours after birth. Twenty multiparous and 10 primiparous dairy goats were enrolled. Behaviors such as walking, isolation, aggression, eating, inactivity and rumination were recorded in females during the last three days prior to the day of delivery, as well as the behaviors of the dam and her progeny in the first 2 h postpartum and the ability of the mother to discriminate between own kid and the alien one (selectivity test) at 2 h postpartum. Gestational and postpartum plasma progesterone and estradiol concentrations were determined. In the prepartum period there was a higher percentage of primiparous goats that were eating and remained less inactive compared to multiparous goats (p < 0.05). During the postpartum period, the lick latency to the kid was shorter in multiparous than in primiparous goats (p = 0.015). There was a greater proportion of kids born from multiparous (67%) than from primiparous (27%) that stood up in the first 30 min postpartum (p < 0.0001). Therefore, the proportion of multiparous kids (33%) than primiparous kids (9%) that were nursed within the first hour after birth was also higher (p < 0.0001). Progesterone and estradiol concentrations were not affected by maternal experience, neither in the prepartum nor the postpartum. In conclusion, maternal experience affected some behaviors at peripartum of the dam and vitality in the kid; however, the differences in behavior were not accompanied by differences in the variations in circulating estradiol and progesterone levels in the dam.

Uterine disease in dairy cows is associated with contemporaneous perturbations to ovarian function

Postpartum uterine disease in dairy cows affects ovarian function, but it is unclear how the type and timing of disease relates to ovarian function. To explore associations between uterine disease and contemporaneous ovarian function, postpartum uterine health was evaluated in 17 lactating primiparous Holstein cows that ovulated. Ovarian function was assessed by measuring daily hormone concentrations and the diameter of the largest follicle and subsequent corpus luteum. Metritis (≤14 d postpartum) was associated with reduced follicle diameter (P = 0.002) and tended to reduce plasma estradiol concentrations (P = 0.062) from d 7 to 16 postpartum compared to cows without metritis. Metritis was associated with reduced plasma progesterone (P = 0.026) but not corpus luteum diameter from d 17 to 26 postpartum. Endometritis (≥15 d postpartum) was associated with reduced corpus luteum diameter (P = 0.005) but did not alter plasma progesterone from d 17 to 26 postpartum compared to cows without endometritis. To explore longer term effects of uterine infection on luteal function, non-lactating primiparous Holstein cows received an intrauterine infusion of vehicle control (n = 11) or pathogenic Escherichia coli and Trueperella pyogenes to induce endometritis (n = 12) and corpora lutea were recovered on d 16 of the estrous cycle, 146 d after intrauterine infusion. Intrauterine infusion of bacteria had no effect on plasma progesterone or luteal diameter compared to control, and only altered the expression of 2 of 94 candidate genes (NCF1 and TLR9). Taken together, these studies imply that uterine diseases are principally associated with changes to ovarian function at a time contemporaneous with disease.

Aromatase, testosterone, TMPRSS2: determinants of COVID-19 severity

BackgroundMale sex has been identified as a risk factor for worse COVID-19 outcomes. This sex difference has been mostly attributed to the complex role of sex hormones. Cell surface entry of SARS-CoV-2 is mediated by the transmembrane protease serine 2 (TMPRSS2) which is under transcriptional regulation by androgens. P450 aromatase enzyme converts androgens to estrogens. This study measured concentrations of aromatase enzyme, testosterone, estradiol, and TMPRSS-2 in plasma of hospitalized COVID-19 patients to elucidate the dynamics of sex-linked disparity in COVID-19 and correlate them with disease severity and mortality.MethodsIn this prospective cohort study, a total of 265 patients (41% women), age 18 years and older, who had a positive COVID-19 PCR test and were hospitalized for COVID-19 at Memorial Hermann Hospital in Houston, (between May 2020 and May 2021) were enrolled in the study if met inclusion criteria. Plasma concentrations of Testosterone, aromatase, TMPRSS-2, and estradiol were measured by ELISA. COVID-19 patients were dichotomized based on disease severity into moderate-severe (n = 146) or critical (n = 119). Mann Whitney U and logistic regression were used to correlate the analytes with disease severity and mortality.ResultsTMPRSS2 (2.5 ± 0.31 vs. 1.73 ± 0.21 ng/mL, p < 0.01) and testosterone (1.2 ± 0.1 vs. 0.44 ± 0.12 ng/mL, p < 0.01) were significantly higher in men as compared to women with COVID-19 after adjusting for age in a multivariate model. There was no sex difference seen in the level of estradiol and aromatase in COVID-19 patients. TMPRSS2 and aromatase were higher, while testosterone was lower in patients with increased COVID-19 severity. They were independently associated with COVID-19 severity, after adjusting for several baseline risk factors in a multivariate logistic regression model. In terms of mortality, TMPRRS2 and aromatase levels were significantly higher in non-survivors.ConclusionsOur study demonstrates that testosterone, aromatase, and TMPRSS2 are markers of COVID-19 severity. Estradiol levels do not change with disease severity in COVID-19. In terms of mortality prediction, higher aromatase and TMPRSS-2 levels can be used to predict mortality from COVID-19 in hospitalized patients.Plain English SummaryCOVID-19 has caused over a million deaths in the U.S., with men often getting sicker than women. Testosterone, a male hormone, helps control a protein called TMPRSS-2, which allows the COVID-19 virus to spread more easily in the body. A protein called aromatase converts the male hormone testosterone into the female hormone estrogen. It is thought that female hormone estrogen helps protect women from getting seriously ill from COVID-19. To understand the role of these hormones in COVID-19 and sex differences, we measured levels of testosterone, estrogen, aromatase (which turns testosterone into estrogen), and TMPRSS-2 in hospitalized COVID-19 patients. We also checked how this level might reflect the severity of the disease. We found that critically ill COVID-19 patients (the ones in ICU) had higher levels of TMPRSS-2 and aromatase, and lower testosterone levels. When we used these hormone levels to predict death in hospitalized COVID-19 patients, higher levels of TMPRSS-2 and aromatase were linked to a lower chance of survival.

Dicliptera laxata (Acanthaceae) (C. B. Clark) Unblocks an Abamectin-Blocked Estrous Cycle and Restores Cyclicity in Female Albino Rats

The farmers of the Buea municipality, Fako Division, South-West Region, Cameroon extensively use Abamectin or Aba, a phytosanitary insecticide which targets insects such as mites, thrips species and leaf miners. In this area, the local population also uses Dicliptera laxata traditionally as a fertility enhancer and in stabilizing risky pregnancies in women. This study was aimed at evaluating the actions of Dicliptera laxata aqueous extract on the estrous cycle and reproductive system of Aba-treated female rats. A total of 30 adult female rats of the confirmed regular cycle were daily treated orally with a 2 ml/kg Abamectin for a period of 45 days, accompanied by the evaluation of the cytology of their vaginal smears at 5 days intervals. From the 46th day, they were subsequently treated with the leaf-aqueous extract of Dicliptera laxata for 15 days and were terminated on the 61st day during which blood and reproductive organs were collected for biochemical assays and relative weight indices. Chronic (45 days) exposure of normal cycled female rats to Abamectin witnessed a blockade in their estrous cycle, a significant drop in plasma estradiol and progesterone concentration, and a significant drop in the percentage weight of organs. Administration of Dicliptera laxata leaf-aqueous extract to these Aba-treated females resulted in a return to the regularity of their estrous cycle, with animals receiving the 300 mg/kg dose of plant extract recording a 60% regulation in the first week and 100% in the second week as compared to animals treated with distilled water that recorded a 0% and 20% regulation in the first and second week of treatment, respectively. Also, the animals that received 300 mg/kg body weight of the extract registered significant (p &lt; 0.05) rise in plasma concentrations of progesterone and estradiol (36.44 ± 1.69 pg/mL and 77.73 ± 3.82 pg/mL, respectively) as compared to animals that received distilled water which showed 22.54 ± 2.19 pg/mL plasma progesterone and a 44.90 ± 7.63 pg/mL plasma estradiol concentrations, respectively. The actions of the leaf-aqueous extract of D. laxata could be attributed to the presence of phytochemicals including steroids, phenolic compounds, alkaloids and saponins in our extract, which have been reported to have important pharmacological effects on female reproduction. D. laxata, therefore, constitutes a potential treatment of female infertility and our findings justify its folkoric use by the Bakweri tribe in enhancing fertility. It can be used to stalize risky pregnancies and prevent miscarriages.

Bioequivalence study of low dose drospirenone/ethinyl estradiol 3 mg/0.03 mg film tablets under fasting conditions in Turkish healthy female subjects.

This bioequivalence research aims to evaluate the relative bioavailability and pharmacokinetic characteristics of ethinyl estradiol and drospirenone in the test preparation in comparison to the reference preparation during fasting conditions. A liquid chromatography method with tandem mass spectrometry was used to determine the concentrations of drospirenone and ethinyl estradiol in plasma. The pharmacokinetic parameters that were analyzed were the maximum plasma concentration (Cmax), time to achieve Cmax (tmax), elimination half life, and area under the concentration time curve of plasma (AUC0-t, AUC0-∞ for ethinyl estradiol, and AUC0-72h for drospirenone). Both the AUC and Cmax parameters were determined to be between 80.00% and 125.00% (90% confidence intervals), which is the acceptable range. Based on the study findings, it was concluded that the test formulation, which includes 3 mg of drospirenone and 0.03 mg of ethinyl estradiol, demonstrated bioequivalence when compared to the reference formulation.

Concentrations of plasma estradiol, progesterone, melatonin, serotonin and nitric oxide in Tuj ewes after estrus syncronization in and out of breeding season

Concentrations of plasma estradiol, progesterone, melatonin, serotonin and nitric oxide in Tuj ewes after estrus syncronization in and out of breeding season

Alcohol intake and endogenous sex hormones in women: Meta-analysis of cohort studies and Mendelian randomization.

The mechanisms underlying alcohol-induced breast carcinogenesis are not fully understood but may involve hormonal changes. Cross-sectional associations were investigated between self-reported alcohol intake and serum or plasma concentrations of estradiol, estrone, progesterone (in premenopausal women only), testosterone, androstenedione, dehydroepiandrosterone sulfate, and sex hormone binding globulin (SHBG) in 45 431 premenopausal and 173 476 postmenopausal women. Multivariable linear regression was performed separately for UK Biobank, European Prospective Investigation into Cancer and Nutrition, and Endogenous Hormones and Breast Cancer Collaborative Group, and meta-analyzed the results. For testosterone and SHBG, we also conducted Mendelian randomization and colocalization using the ADH1B (alcohol dehydrogenase 1B) variant (rs1229984). Alcohol intake was positively, though weakly, associated with all hormones (except progesterone in premenopausal women), with increments in concentrations per 10 g/day increment in alcohol intake ranging from 1.7% for luteal estradiol to 6.6% for postmenopausal dehydroepiandrosterone sulfate. There was an inverse association of alcohol with SHBG in postmenopausal women but a small positive association in premenopausal women. Two-sample randomization identified positive associations of alcohol intake with total testosterone (difference per 10 g/day increment: 4.1%; 95% CI, 0.6-7.6) and free testosterone (7.8%; 4.1-11.5), and an inverse association with SHBG (-8.1%; -11.3% to -4.9%). Colocalization suggested a shared causal locus at ADH1B between alcohol intake and higher free testosterone and lower SHBG (posterior probability for H4, 0.81 and 0.97, respectively). Alcohol intake was associated with small increases in sex hormone concentrations, including bioavailable fractions, which may contribute to its effect on breast cancer risk.

102 Estradiol and follicular growth are associated with estrous behavior monitored by an accelerometer in grazing beef cows

Abstract In beef cattle, 40% of cows that display estrus do not remain pregnant after embryo transfer (ET). Estrus is a physiological behavior influenced by the concentration of sex-steroids. The use of accelerometer devices for estrus detection is a common practice in dairy operations. It remains unclear if this same artificial intelligence software can accurately measure reproductive behavior in beef females. We hypothesized that estrous variables detected by an accelerometer are associated with ovarian, hormonal, pregnancy outcome, and fertility-related variables measured in the cow. Fifty Bos indicus-influenced, cyclic, primiparous cows were fitted with an accelerometer collar (SCR Engineers, Israel) and a breeding indicator (Estrotectâ) to detect estrus after a synchronization protocol in five subsequent experimental replicates (n = 50*5 = 250). Only cows that showed estrus (d 0) remained in the study. The following variables were used from the accelerometer software: Activity Peak, Rumination Nadir, Estrus Duration, and Heat Index, calculated by proprietary algorithms. Dominant follicle (DF) diameter and plasma concentration of estradiol were measured on d -2 (day of progesterone device removal) and on d 0. On d -1 and d 4, endometrial thickness and luminal fluid accumulation were measured by ultrasonography. On d 7, two embryos were transferred/cow. Pregnancies were diagnosed on d 45 and terminated. After 30 d, a new replicate started. Cows included in at least two replicates that remained pregnant 100%, 99-50%, or less than 50% were classified as fertile, sub fertile, and infertile, respectively. Estrus display was recorded for 166 cows (66%). Estrus detection by accelerometer and Estrotect had excellent agreement rate (kappa coefficient 0.76). Cows detected in estrus only by Estrotect had 66% less pregnancy/ET than cows in estrus by both (9/21 vs. 97/145; P = 0.01). The DF diameter on d 0 was not associated with accelerometer variables. Cows with greater and moderate Activity Peak had a greater growth rate of the DF from d -2 to d 0 than lower Activity Peak cows (interaction day*Activity Peak; P = 0.06). Estradiol on d -1 was positively associated with the Activity Peak (P &amp;lt; 0.0001). Cows with lower Activity Peak had a thicker endometrium on both d -1 and d 4 (P=0.02), and greater uterine luminal fluid score on d -1 (P=0.03). Fertility status was not associated with any accelerometer variables. In conclusion, the Activity Peak is associated positively with estradiol concentration and DF growth, however no association was found with pregnancy outcome and fertility. Therefore, the main advantage of accelerometers in beef ranches is the ability to detect estrus at a similar accuracy as breeding indicators, using less labor. Future enhancements in hardware and software to allow prediction of pregnancy outcomes are expected to increase reproductive efficiency in cow-calf operations.

Sex-steroid hormones and risk of postmenopausal estrogen receptor-positive breast cancer: a case-cohort analysis.

Sex-steroid hormones are associated with postmenopausal breast cancer but potential confounding from other biological pathways is rarely considered. We estimated risk ratios for sex-steroid hormone biomarkers in relation to postmenopausal estrogen receptor (ER)-positive breast cancer, while accounting for biomarkers from insulin/insulin-like growth factor-signaling and inflammatory pathways. This analysis included 1208 women from a case-cohort study of postmenopausal breast cancer within the Melbourne Collaborative Cohort Study. Weighted Poisson regression with a robust variance estimator was used to estimate risk ratios (RRs) and 95% confidence intervals (CIs) of postmenopausal ER-positive breast cancer, per doubling plasma concentration of progesterone, estrogens, androgens, and sex-hormone binding globulin (SHBG). Analyses included sociodemographic and lifestyle confounders, and other biomarkers identified as potential confounders. Increased risks of postmenopausal ER-positive breast cancer were observed per doubling plasma concentration of progesterone (RR: 1.22, 95% CI 1.03 to 1.44), androstenedione (RR 1.20, 95% CI 0.99 to 1.45), dehydroepiandrosterone (RR: 1.15, 95% CI 1.00 to 1.34), total testosterone (RR: 1.11, 95% CI 0.96 to 1.29), free testosterone (RR: 1.12, 95% CI 0.98 to 1.28), estrone (RR 1.21, 95% CI 0.99 to 1.48), total estradiol (RR 1.19, 95% CI 1.02 to 1.39) and free estradiol (RR 1.22, 95% CI 1.05 to 1.41). A possible decreased risk was observed for SHBG (RR 0.83, 95% CI 0.66 to 1.05). Progesterone, estrogens and androgens likely increase postmenopausal ER-positive breast cancer risk, whereas SHBG may decrease risk. These findings strengthen the causal evidence surrounding the sex-hormone-driven nature of postmenopausal breast cancer.

Circulating endogenous sex steroids and risk of differentiated thyroid carcinoma in men and women.

Thyroid cancer (TC) is substantially more common in women than in men, pointing to a possible role of sex steroid hormones. We investigated the association between circulating sex steroid hormones, sex hormone binding globulin (SHBG) and the risk of differentiated TC in men and women within the European Prospective Investigation into Cancer and nutrition (EPIC) cohort. During follow-up, we identified 333 first primary incident cases of differentiated TC (152 in pre/peri-menopausal women, 111 in post-menopausal women, and 70 in men) and 706 cancer-free controls. Women taking exogenous hormones at blood donation were excluded. Plasma concentrations of testosterone, androstenedione, dehydroepiandrosterone, estradiol, estrone and progesterone (in pre-menopausal women only) were performed using liquid chromatography/mass spectrometry method. SHBG concentrations were measured by immunoassay. Odds ratios (ORs) were estimated using conditional logistic regression models adjusted for possible confounders. No significant associations were observed in men and postmenopausal women, while a borderline significant increase in differentiated TC risk was observed with increasing testosterone (adjusted OR T3 vs T1: 1.68, 95% CI: 0.96-2.92, ptrend = .06) and androstenedione concentrations in pre/perimenopausal women (adjusted OR T3 vs T1: 1.78, 95% CI: 0.96-3.30, ptrend = .06, respectively). A borderline decrease in risk was observed for the highest progesterone/estradiol ratio (adjusted OR T3 vs T1: 0.54, 95% CI: 0.28-1.05, ptrend = .07). Overall, our results do not support a major role of circulating sex steroids in the etiology of differentiated TC in post-menopausal women and men but may suggest an involvement of altered sex steroid production in pre-menopausal women.

Intervening effects of progesterone combined with estradiol on streptozotocin-induced diabetes in ovariectomized mice and the underlying mechanisms

Background: Estrogen and progesterone are closely related to the occurrence of diabetes, and the progestogenic and estrogenic effects in the development of diabetes are related to their blood levels and the organism state, and it is not clear what effect their combination will be. The present study was to investigate the interaction between estrogen and progesterone in the development of diabetes mellitus. Methods: A model of type 1 diabetes mellitus（T1DM）was established in ovariectomized (OVX) mice by intraperitoneal injection of streptozotocin (STZ), and the levels of plasma glucose, insulin, C-peptide, progesterone, and estradiol, the changes of islet cells, the expressions of glucose transporter 4 (GLUT4), Glucokinase (Gck), Glucose-6-phosphatase (G-6-P) and phosphoenolpyruvate carboxylase (PCK) were detected after chronically injected different doses of progesterone and 17β-estradiol subcutaneously for 4 weeks. Results: Progesterone and 17β-estradiol could delay the occurrence of T1DM induced by STZ in ovariectomized mice, but there was no dose-response relationship; progesterone (2mg/kg) and 17β-estradiol (0.1mg/kg) injected separately or jointly could make the plasma concentration of estradiol and progesterone reaches the physiological dose range, which markedly inhibited the increase of the blood glucose induced by STZ, improved the glucose tolerance, protected islet cells, promoted C-peptide and insulin secretion, up-regulated skeletal muscle GLUT4 and liver Gck expressions, but significantly down-regulated the expressions of liver PCK and G-6-P mRNA. Conclusions: The physiological dose of progesterone combined with estradiol has certain synergistic effects in delaying the occurrence of diabetes, the mechanisms are probably related to the protection of islet cells, the promotion of insulin release, skeletal muscle glucose transport, and liver glucose metabolism.

Витамин D и овариальный резерв у пациенток с эндометриозом, перенесших новую коронавирусную инфекцию

Aim: to study the effect of vitamin D on ovarian reserve in women with novel coronavirus infection (COVID-19). Patients and Methods: 137 women aged 25–35 years with infertility associated with lack of ovulation (ICD: N97.0) presenting as hypergonadotropic, hypoestrogenic anovulation were evaluated and treated. Of this sample, 67 women had laboratory-confirmed SARS- CoV-2 infection and 70 women had no history of infection. All women in the sample had serum vitamin D insufficiency or deficiency. The women were divided into 2 groups; each group was divided into 2 subgroups. Group 1 (n=67) included women with a history of COVID-19. Group 2 (n=70) included women without a history of COVID-19. In addition to standard infertility treatment, 35 and 34 women in groups 1 and 2, respectively, received vitamin D supplementation (Fortedetrim), the dosage of which was determined according to the manufacturer's instructions based on the severity of vitamin D insufficiency (these women were included in treatment sub-groups 1A and 2A). Results: women who experienced COVID-19 had higher follicle-stimulating hormone (FSH) levels and lower plasma estradiol concentrations than uninfected women. In these women, ultrasound also showed a decrease in the number of follicles per slice and in ovarian volume. Vitamin D improved reproductive function in women with a history of COVID-19 by decreasing FSH levels, increasing estradiol levels, and increasing antral follicle count and ovarian volume. No treatment-emergent adverse events, including clinically significant abnormalities in blood and urine tests, were reported. Conclusion: vitamin D supplementation in women of reproductive age with a history of COVID-19 and reduced ovarian reserve is a clinically effective and safe tool for the management of reproductive disorders. KEYWORDS: infertility, ovarian reserve, COVID-19, vitamin D, antral follicles, follicle-stimulating hormone, estradiol. FOR CITATION: Kalimatova D.M., Dobrokhotova Yu.E., Il'ina I.Yu. Vitamin D and ovarian reserve in women with endometriosis who experienced novel coronavirus infection. Russian Journal of Woman and Child Health. 2024;7(3):199–204 (in Russ.). DOI: 10.32364/2618-8430-2024-7-3-2.

VERAPAMIL, NIFEDIPIN VƏ DILTIAZEMIN QANIN PLAZMASINDA ESTRADIOLUN QATILIĞINA TƏSIRININ TƏDQIQI

The effect of long-term intake of calcium antagonists on the concentration of estradiol in the blood was studied. The studies were carried out on 82 female white rats, grown in laboratory conditions, weighing 180-230 grams, which have reached puberty at the Scientific Research Center of the Azerbaijan Medical University. Verapamil 5, 25 mg/kg (Isoptin, Abbot Laborato-ries S.A., Italy), nifedipine 5, 10 mg/kg (Farmadipin, Farmak Ukraine), diltiazem 5, 20 mg/kg (Diltizem-L, MNIS-Istanbul ) were used in the research. ) was used. The concentration of estra-diol in blood plasma was determined by enzyme immunoassay using the BioScreen MS-500 de-vice. Blood was taken from the hearts of experimental animals under ether anesthesia. To de-termine the concentration of the hormone estradiol in the blood, a set of reagents "Eliza Kit Es-tradiol" was used. Estradiol levels were 3.9% lower and 40.4% higher with verapamil (5 mg/kg) compared with control females in females receiving long-term treatment with nifedipine at a dose of 5 mg/kg. With long-term use of diltiazem at a dose of 5 mg/kg, the concentration of es-tradiol differed by 3.3% (p&gt;0.05). With a corresponding increase in doses, nifedipine 10 mg/kg (65.0±2.4) &gt; diltiazem 20 mg/kg (39.3±0.7) &gt; verapamil 25 mg/kg (34.3±0.7) increased the con-centration estradiol.

In ovo o,p'-DDT exposure induces malformation of reproductive organs and alters the expression of genes controlling sexual differentiation in Japanese quail embryo.

In ovo exposure to o,p'-dichloro-diphenyl-trichloroethane (o,p'-DDT) impairs reproduction by inducing malformation of the reproductive organs in birds, although the mechanism remains unclear. Here, we examined the effects of o,p'-DDT on the development of the reproductive organs, the expression of genes controlling sexual differentiation, and the plasma concentrations of testosterone and estradiol in Japanese quail embryos. o,p'-DDT-containing sesame oil was injected into the yolk sac on Embryonic Day (E) 3 at a dose of 500, 2,000, or 8,000 μg per egg. On E15, the reproductive organs were observed; the gonads and Müllerian ducts (MDs) were sampled to measure the mRNA of steroidogenic enzymes, sex steroid receptors, anti-Müllerian hormone (AMH), and AMH receptor 2 (AMHR2); blood samples were collected to assay plasma testosterone and estradiol levels; and the gonads were used for histological analysis. o,p'-DDT dose-dependently increased the prevalence of hypertrophic MDs in females and residual MDs in males. In female MDs, o,p'-DDT dose-dependently decreased estrogen receptor (ER) α, ERβ, and AMHR2 mRNA expression. o,p'-DDT dose-dependently induced left-biased asymmetry of testis size, and ovary-like tissue was found in the left testis after exposure to 8,000 μg per egg o,p'-DDT, although asymmetric gene expression did not occur. o,p'-DDT did not affect ovarian tissue but did decrease 17α-hydroxylase/C17-20 lyase mRNA expression and dose-dependently increased ERβ mRNA expression. o,p'-DDT decreased plasma testosterone concentrations in females. These findings suggest that o,p'-DDT induces hypertrophy of the MDs and ovarian tissue formation in the left testis. Abnormal MD development may be linked to altered gene expression for sensing estrogens and AMH signals.

Frequency and Characteristics of Postoperative Neuropathy in Individuals on Gender-Affirming Hormone Therapy Undergoing Gender Affirmation Surgery: A Retrospective Cohort Study.

Introduction Gender affirmation surgery includes procedures of the face, larynx, chest, reproductive system, external genitalia, and adipose tissue performed to ameliorate incongruence between gender identity and phenotype. The annual number of gender affirmation surgeries performed in the United States has increased significantly. There have been no investigations into the frequency of peripheral neuropathy after gender affirmation surgery, which is an important topic, given transgender individuals are at increased risk for delaying necessary medical care. After appreciating a number of cases of postoperative neuropathy in our clinical practice, we hypothesized that gender affirmation surgery is a high-risk procedure for postoperative neuropathy. Methods We conducted a one-year, monocentric, retrospective cohort study utilizing clinical data of individuals on gender-affirming hormone therapy undergoing gender-affirmation surgery under general anesthesia. The study included transgender women, assigned male at birth, receiving antiandrogen, progesterone, or estrogen therapy (target range plasma estradiol concentration 90-200 pg/ml) and transgender men, assigned female at birth, receiving antiestrogen or testosterone therapy (target range plasma testosterone concentration 320-1000 ng/dl). The primary objective was to estimate the incidence of postoperative peripheral neuropathy, defined as new numbness, paresthesia, neuropathic pain, or muscle weakness occurring in a peripheral innervation territory. Secondary objectives were to summarize the clinical presentation of neuropathy and investigate for associations between procedure characteristics and neuropathy. Results We identified nine cases of postoperative peripheral nerve injury in 232 consecutive gender affirmation procedures establishing an incidence of 3.9%. All injuries were associated with surgery longer than six hours and were characterized by sensory deficits including numbness 89% (8/9) and tingling 56% (5/9), which were diagnosed by postoperative day one. Conclusions Our results suggest that gender affirmation surgery is a high-risk procedure for postoperative neuropathy, with an incidence similar to other high-risk procedures, and an incidence that is higher than the general surgical population. However, given this has not been previously reported and our study includes a heterogenous population from a single institution, our results should be considered hypothesis generating. Additional studies that include multiple institutions are needed to confirm our findings and identify modifiable risk factors for postoperative neuropathy.

Unveiling the Effects of Fennel (Foeniculum vulgare) Seed Essential Oil as a Diet Supplement on the Biochemical Parameters and Reproductive Function in Female Common Carps (Cyprinus carpio)

The present study aimed to investigate the effect of Foeniculum vulgare essential oil on the health of the common carp (Cyprinus carpio). A total of 120 healthy fish were provided with feed containing 200, 400, and 600 mg/kg of F. vulgare oil for 60 days. Findings revealed that the oral administration of 200 mg/kg of F. vulgare oil significantly increased final weight, weight gain, and specific growth rate compared to the control group (p &lt; 0.05). Plasma biochemical enzyme levels remained unchanged in 200 and 400 mg/kg treatments, whereas they were found to be significantly increased in treatments with 600 mg/kg. Although no significant alterations in glucose, triglyceride, and cholesterol levels were observed, the treated groups exhibited significant increases in total protein, albumin, globulin, and creatinine levels. Results also indicated significantly decreasing levels of glutathione peroxidase, whereas superoxide dismutase activity increased. The gonadosomatic index showed notable improvement in the 200 and 400 mg/kg groups. Furthermore, plasma concentrations of estradiol and testosterone were significantly affected by doses of 400 and 200 mg/kg. Findings suggest that, following the administration of F. vulgare extract, the reproductive and general health of the fish appears to be improved. Nevertheless, it is recommended to supplement fish diets with up to 200 mg/kg of F. vulgare extract to improve their reproductive and general health. Concentrations above this limit can potentially cause harm.

Plasma estradiol profile after administration of different types of estradiol esters in acyclic mares.

Although the use of different estradiol esters has been extensively studied in hormonal protocols in cows, such information is lacking in mares. The present study aimed to assess the effects of treatment with the same doses and administration frequency of estradiol cypionate, estradiol benzoate and 17β estradiol on plasma estradiol (E2) concentrations of acyclic mares and correlate the E2 profile to the endometrial edema score. Sixteen treatments were performed in 14 mares randomly divided into three groups: EB (n=5), EC (n=5) and 17β (n=6), receiving 10mg on day 0 (D0), 6mg on D1 and 4mg on D2 of estradiol benzoate, estradiol cypionate, and estradiol 17β, respectively. Blood samples, rectal palpations and ultrasound evaluations were performed once daily, starting before the first estradiol treatment (D0) until edema disappearance or D8. Moderate to high edema was observed in all groups 24h after the first estradiol administration. Edema persisted above score 2 until D7, D5 and D4 in groups EC, EB and 17β, respectively. Higher edema was found on D2 in EB group compared to 17β, on D6 and D7 in EC compared to 17β, and on D8 in EC compared to EB (p≤0.05). Maximum E2 concentrations were detected on D1 in groups: EB, showing a sharp decrease from D2 to D3 (p<0.0001); and 17β, where no differences were observed between treatment days (p≥0.05). In the EC group, maximum concentrations were observed on D2, which remained high from D2 to D4 compared to the other days (p<0.0001). Plasma concentration of E2 was higher in EB than the other groups on D0 and D1, and EB>EC>17β on D2. Plasma E2 concentrations and edema score were positively correlated, being moderate in groups EB and EC and weak in the 17β group. In conclusion, the most pronounced plasma E2 concentration was observed in EB group 24h after the administration of the first dose. Estradiol concentrations peaked 48h after EC administration, while a distinct peak was not observed in a 24h interval evaluation in the 17β group. Moreover, high edema does not necessarily reflect on high E2 concentrations in acyclic mares.

CANNIBALISM PERFORMANCE OF ASIAN REDTAIL CATFISH (Hemibagrus nemurus) FED RATION SUPPLEMENTED WITH DIFFERENT DOSES OF 17α-METHYLTESTOSTERONE

One of the obstacles in Asian redtail catfish hatchery is the high cannibalism incidence. Cannibalism is associated with aggressive behavior caused by hormonal metabolism especially of testosterone. The purpose of this study was to evaluate the effect of testosterone administration on the cannibalism incidence in the Asian redtail catfish juveniles. The experiment was conducted by rearing fish with initial length of 4.09±0.19 cm in 16 of 20 L aquariums with a density of 2 fish L-1. The experiment was designed with a completely randomized design with 4 treatments which were different level of 17α-methyltestosterone supplementation in feed, i.e., 0 mg kg-1 feed (A) as control, 7.5 mg kg-1 feed (B), 15 mg kg-1 feed (C), and 30 mg kg-1 feed (D). Each treatment has 4 replications. Fish fed experimental diet (40% protein) 4 times a day to satiation for 30 days. The parameters observed were type and index of cannibalism, aggressive behavior, survival rate, normal mortality, growth performance, hormones concentrations (estradiol, testosterone, and cortisol), and water quality. The results showed that cannibalism type II (the fish eaten completely or missing) and cannibalism index increased with the increasing doses of testosterone administration in the feed with the highest cannibalism incidence was 40.63%. The highest survival rate was found in treatment B (73.75±2.50%) and was not significantly different from the control treatment (69.38±2.39%). No differences in testosterone concentration and in the growth performance among the treatments. However, there was a trend of decrease in the estradiol concentration of Asian redtail catfish juveniles fed ration supplemented with the increasing doses of 17α-methyltestosterone. Based on the results obtained in this research, estradiol changed in the body's plasma, it appears that there was a role for plasma estradiol concentration in controlling cannibalism of Asian redtail catfish juveniles. It concluded that the testosterone administration affected the cannibalism incidence in the Asian redtail Catfish.

Plasma estradiol profile after administration of different types of estradiol esters in acyclic mares.

Although the use of different estradiol esters has been studied extensively in hormonal protocols in cows, such information is lacking in mares. The present study aimed to assess the effects of treatment with the same doses and administration frequency of estradiol cypionate (EC), estradiol benzoate (EB) and 17β estradiol (17β) on plasma estradiol (E2) concentrations of acyclic mares and correlate the E2 profile to the endometrial edema score. Sixteen treatments were performed in 14 mares randomly divided into three groups: EB (n=5), EC (n=5) and 17β (n=6), receiving 10mg on day 0 (D0), 6mg on D1 and 4mg on D2 of estradiol benzoate (Sincrodiol®, Ouro Fino, Brazil), estradiol cypionate (E.C.P®, Zoetis, Brazil), and estradiol 17β (17βeta®, Botupharma, Brazil), respectively. Blood samples, rectal palpations and ultrasound evaluations were performed once daily, starting before the first estradiol treatment (D0) until edema disappearance or D8. Plasma E2 was quantified by radioimmunoassay. Endometrial edema was scored as: 0= lack of edema, 1=mild, 2=moderate, 3=high and 4=exaggerated. Mean estradiol plasma concentrations were analyzed by general linear model of variance with repeated measures. Median edema scores were compared by Kruskal Wallis followed by Mann Whitney tests while Spearman's correlation test was used to correlate E2 concentrations and edema score. Moderate to high edema was observed in all groups 24h after the first estradioladministration. Edema persisted above score 2 until D7, D5 and D4 in groups EC, EB and 17β, respectively. Higher edema was found on D2 in group EB compared to 17β, on D6 and D7 in group EC compared to 17β, and on D8 in group EC compared to EB (p≤0.05). Maximum E2 concentrations were detected on D1 in groups: EB (190±83.3 pg/mL), showing a sharp decrease from D2 to D3 (p<0.0001); and 17β (44.99±15.0 pg/mL), where no differences were observed between treatment days (p≥0.05). In the EC group, maximum concentrations were observed on D2 (81±43.8 pg/mL), which remained high from D2 to D4 compared to the other days (p<0.0001). Plasma concentration of E2 was higher in group EB than the other groups on D0 and D1, and EB>EC>17β on D2. Plasma E2 concentrations and edema scores were positively correlated, being moderate in groups EB and EC (EB: r=0.47; EC: r=0.45, p<0.01) and weak in the 17β group (r=0.39, p<0.0001). In conclusion, the most pronounced plasma E2 concentration was observed in group EB 24h after the administration of the first dose. Estradiol concentrations peaked 48h after EC administration, while a distinct peak was not observed in a 24h interval evaluation in the 17β group. Moreover, high edema does not necessarily reflect on high E2 concentrations in acyclic mares.

Impact of growth trajectory on sexual maturation in layer chickens.

Recent studies showed that apart from photostimulation, metabolic triggers may independently activate sexual maturation and egg production in chickens. However, the origin, mode of action, and specific target(s) of this metabolic control remain unknown. Beyond body weight (BW), we hypothesize that body composition (BC) and associated specific metabolic signals are involved. Thus, this study was conducted to determine the BW and BC thresholds triggering spontaneous sexual maturation in layer pullets under different growth trajectories. Day-old Lohman LSL lite and Lohman brown lite chicks (n = 210 each) raised in brooding cages under ad libitum (AL) feeding until 8weeks of age were randomly allocated into individual cages and assigned to one of 3 experimental growth profiles; AL, breeder's target (T), restricted 20% below target (R), (n = 70 birds/profile/strain). Birds had free access to water throughout the trial. All hens were maintained on 10h of light (10lux) throughout the rest of the study. Blood and tissue samples were collected throughout the study to measure plasma estradiol (E2) concentrations and organ weights, respectively. Furthermore, carcasses were subjected to Dual-energy X-ray absorptiometry (DEXA) analyses. All analyses were completed with SAS using the MIXED procedure. Results show that R treatment slowed (p < 0.001) growth, delayed age at first egg (FE) and egg production (p < 0.001) and resulted in lower BW at FE (p < 0.001), lower ovary weight and number of follicles (p < 0.001) compared to AL in both strains, whereas, the strain significantly impacted body weight (p < 0.0001), ovary weight (p < 0.001), BW at FE (p < 0.001), age at FE (p < 0.001), egg production (p < 0.0001), E2 (p < 0.0001) and body composition (p < 0.05). For DEXA, AL feeding (p < 0.001) increased fat deposition compared to R. Furthermore, there was a positive correlation between plasma E2 and bone mineral content (p < 0.01) and bone mineral density (p < 0.01). In conclusion, feed allocation impacted growth and BC in a strain dependent manner which resulted in differing age at sexual maturation and egg production. Furthermore, a body fat threshold between 10% to 15% appears to be required for the occurrence of spontaneously sexual maturation in laying hens.