17α-ethinylestradiol (EE2), an endocrine disruptor (ED) excreted by women and is found in contraceptive and hormone replacement pills used at menopause. An increase in EDs requires alternative means of water and sewage treatment, since these compounds threaten human health and the environment, even at trace levels. Phytotechnology stands out from other processes because it is sustainable, given that plants are used to recover the contaminated environment. Thus, the objective of this study was to evaluate rice grass performance in the removal of 17α-ethinylestradiol (EE2), and determine the effect of this hormone on the root anatomy and chlorophyll content of the species. A completely randomized design was used with 4 replications: EE2 concentration composed of 4 study treatments (420, 300, 180 and 60 μg L-1 EE2) and 2 control treatments (positive and negative). The effect of the treatments was evaluated over time (1, 7 and 15 days). EE2 removal from the solution was analyzed by HPLC. Chlorophyll was analyzed by UVvis spectrophotometry, showing that EE2 does not interfere with the photosynthetic activity of rice grass. Aerenchyma was analyzed by microphotographs of root sections, revealing a higher proportion in plants exposed to the hormone, indicating morpho-anatomical adaptations to survive in this medium. Thus, it can be concluded that rice grass has significant phytoremediation potential after 7 days’ exposure, surviving and adapting to the contaminated environment, and can be used to complement water and sewage treatments.