Different Concentrations Of Amino Acids Research Articles

Metabolome Profiling in the Plasma of Dogs with Idiopathic Dilated Cardiomyopathy: A Multiplatform Mass-Spectrometry-Based Approach.

Dilated cardiomyopathy is one of the important diseases in dogs and humans. The second most common cause of heart failure in dogs is idiopathic dilated cardiomyopathy (iDCM), which results in heart failure or sudden cardiac death due to arrhythmia. This study aimed to determine changes in the plasma metabolome of dogs with iDCM compared to healthy dogs. For that purpose, a multiplatform mass-spectrometry-based approach was used. In this study, we included two groups of dogs: 12 dogs with iDCM and 8 healthy dogs. A total of 272 metabolites were detected in the plasma samples of dogs by combining three approaches but four MS-based platforms (GC-MS, LC-MS (untargeted), LC-MS (targeted), and FIA-MS (targeted) methods). Our findings demonstrated changes in the canine plasma metabolome involved in the development of iDCM, including the different concentrations of amino acids, biogenic amines, acylcarnitines, triglycerides and diglycerides, sphingomyelins, and organic acids. The results of this study will enable the detection and monitoring of pathophysiological mechanisms involved in the development of iDCM in the future.

Investigation of biochemical traits and mineral element content in the Shahani date fruit under the influence of the foliar application of organic matter and micro-elements

This experiment aimed to investigate the effects of foliar application of organic matter and microelements on the biochemical properties and mineral element content in the Shahani date fruit for two successive years. This was done in the form of randomized complete blocks with 14 treatments in three replications on 42 palms in Jahrom city. Treatments included control (spraying distilled water; amino acids at three concentrations of 0.5, 1.0, and 1.5 gL -1 ; Seaweed at three concentrations of 0.25, 0.5, and 0.75 gL -1 ; microelements at three concentrations of 0.1, 1.5, and 2.0 gL -1 ; four combined treatments obtained from different concentrations of amino acids, seaweed, and microelements. The treatments were applied in two stages (beginning and end of the Kimri stage) on the fruits. The results showed that the treatments had a significant effect on all studied traits except magnesium content in the fruits. The highest copper content in the fruit was observed for the treatment of microelements (2 gL -1 ) (1.63 mgkg −1 ), and the maximum levels of reducing sugar and total carbohydrates (59.60 and 61.84, respectively) were related to the seaweed treatment (0.75), and no significant difference was found with the combined treatment of amino acid + seaweed + microelements (T14), which was in the highest statistical class in the other traits. In general, this combined treatment with the lowest soluble tannin content (26.96) compared to the control (29.40) and other treatments and based on other biochemical traits and the mineral element content in the fruit was the most effective treatment, and its use is recommended to be achieve more desirable results.

Auxotrophs compromise cell growth and fatty acid production in Saccharomyces cerevisiae.

Auxotrophic marker genes have been widely used for genetic engineering in yeast. However, the effects of amino acids or nucleotides deficiency in auxotrophic strains on cell growth and product synthesis were rarely reported. In this study, a total of eight auxotrophic strains of Saccharomyces cerevisiae with single knockout of selection markers were obtained. Cell growth and free fatty acid (FFA) production of these auxotrophic strains were evaluated with supplementation of different concentrations of amino acids or nucleotides. Generally, except ade2Δ mutants, most auxotrophic strains showed decreased cell growth and FFA production, which could be recovered by adding higher concentrations of supplements. LEU2 deletion (leu2Δ) damaged both cell growth and FFA production even with supplementation of 1000mgL-1 leucine. This study shows that growth and product biosynthesis of auxotrophs could be limited by insufficient supplementation of amino acids or nucleotides, and provides guidance on supplementation of these nutrients during fermentation to maximize cell growth and product biosynthesis.

Effect of Different Concentration of Amino Acid and Vitamins in Properties Yield and Components Plant Basil Ocimmum basilicum L.

This study was carried out at a private farm in city Samarra in Qalaa for the 2016 summer season. This experiment included on factors is basil. Different concentrations of amino acid and vitamins and their interaction Amino acid included different concentrations of amino acid aspartic acid and amino acid phenylalanine ethylene the vitamins also included different concentrations of ascorbic acid and vitamins thiamine The experiment was conducted to determine the effect of spraying on different concentrations of amino acid and vitamins in the characteristics of vegetative growth ,physiological characteristics the ratio of the pilot oil , its physical properties and the active substance. The experiment was applied using full Random Sector Design (R.C.B.D) (Complete Black Design Randomized) within the simple trials of three replication the effect of spraying amino acid and vitamins can have a significant effect on most of the studied traits as follows: The treatment (spartic acid 150ppm x ascorbic acid 50 mg.L) was given in most studied traits , giving the number of date flowers (69.5) and number of seeds in the broiler (195.47) and weight 1000 seeds (1.72) and the origin of the leafs (1122.3) and total seed yield (2764.33).
 

Investigating the Effect of Basic Amino Acids and Glucosamine on the Solubility of Ibuprofen and Piroxicam.

Poor aqueous solubility hampers the development of several compounds as pharmacological agents. Hence, preparing novel formulations with augmented absorption is a challenge in pharmaceutical industries. In this paper, we have examined the effect of basic amino acids including arginine (ARG), lysine (LYS), and glucosamine (GlucN) on the solubility of ibuprofen (IBU) and piroxicam (PXM) as drugs with limited solubility. We have also studied the effect of the dissolution media with the pH values 1.2 to 7.4. The saturation shake-flask method was used for solubility studies in the presence of amino acids. Briefly, buffer solutions containing different concentrations of amino acids were prepared. Then, an excess amount of each drug with these buffers was shaken to reach equilibrium. After 48 hours, the upper phase was separated, and solubility was calculated by reading their UV-Vis absorbance. The results illustrated that amino acids increased solubility of both drugs with different ratios, which were pH and concentration-dependent. Solubility improved as the amount of amino acids went up, and this upward pattern was more robust with ARG than LYS. The presence of GlucN in citrate buffer significantly enhanced IBU solubility. The solubility of PXM in accompany of GlucN in water did not change significantly while in citrate buffer solubility enhanced specially at pH 6. Overall, GlucN in citrate buffer and ARG in phosphate buffer could be introduced as the most suitable media for IBU and PXM solubility improvement, respectively.

Characterization of aroma compounds and effects of amino acids on the release of esters in Laimao baijiu.

Laimao baijiu is a typical soy-sauce aroma-type baijiu in China. Amino acids are non-volatile compounds in baijiu and are beneficial to human health. Aroma is one of the important indicators that are used to evaluate the quality of baijiu. The interaction between aroma-active compounds and non-volatile compounds can also affect the release of aroma compounds. In this study, we identified the active-aroma compounds and amino acids in Laimao baijiu by stir bar sorptive extraction (SBSE), gas chromatography-olfactometry (GC-O), gas chromatography-mass spectrometry (GC-MS), and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). The interaction between amino acids and key esters was investigated by sensory analysis and partition coefficients. A total of 63 aroma compounds and 21 amino acids were identified. Twenty-one esters were identified from them as major aroma-active ester compounds with odor activity values ≥ 1. Finally, sensory analysis revealed that l-alanine had a significant effect on the strength of the aromas of esters, suggesting that low concentrations of amino acids were more likely to promote the release of esters and high concentrations were more likely to inhibit this. The partition coefficient can be a good explanation for this phenomenon. l-Alanine can significantly affect the aroma intensity of key ester aroma compounds in Laimao baijiu, and the effects of different concentrations of amino acids are different. This work shows that amino acids, as non-volatile compounds, have a regulatory effect on the release of aroma compounds in alcoholic beverages, which may provide new technical support for the aroma modulation of alcoholic beverages. © 2022 Society of Chemical Industry.

Effects of sulphur amino acids on the size and structure of microbial communities of aerobic granular sludge bioreactors

Granular activated sludge has been described as a promising tool in treating wastewater. However, the effect of high concentrations of sulphur amino acids, cysteine and methionine, in the evolution, development and stability of AGS-SBRs (aerobic granular sludge in sequential batch reactors) and their microbial communities is not well-established. Therefore, this study aimed to evaluate microbial communities' size, structure and dynamics in two AGS-SBRs fed with two different concentrations of amino acids (50 and 100 mg L−1 of both amino acids). In addition, the impact of the higher level of amino acids was also determined under an acclimatization or shock strategy. While N removal efficiency decreased with amino acids, the removal of the organic matter was generally satisfactory. Moreover, the abrupt presence of both amino acids reduced even further the removal performance of N, whereas under progressive adaptation, the removal yield was higher. Besides, excellent removal rates of cysteine and methionine elimination were found, in all stages below 80% of the influent values. Generally considered, the addition of amino acids weakly impacts the microbial communities' total abundances. On the contrary, the presence of amino acids sharply modulated the dominant bacterial structures. Furthermore, the highest amino acid concentration under the shock strategy resulted in a severe change in the structure of the microbial community. Acidovorax, Flavobacterium, Methylophilus, Stenotrophomonas and Thauera stood out as the prominent bacteria to cope with the high presence of cysteine and methionine. Hence, the AGS-SBR technology is valuable for treating influents enriched in sulphur Aa inclusively when a shock strategy was used.

Mass transfer role in electropolishing of carbone steel in H3PO4 containing amino acids: Electrochemical, computational, SEM/EDX, and stylus profilometer investigation

The goal of this study is to look at the development and use of green inhibitors in the electropolishing process (EP), as well as their potential as an anti-corrosive coating for C-steel in an acidic medium. We can replace environmentally dangerous substances with inexpensive, effective molecules that have little or no negative environmental impact like amino acids which are employed as safe inhibitors. The EP characteristics of C-steel were measured by galvanostatic techniques with different concentrations of amino acids over a wide range of (1-1000 × 10−6 mol/l) and temperatures (298–313 K). The findings indicate that the electropolishing behavior of C-steel is governed by mass transfer, with Fe2+ being proposed as the governing species for the salt film mechanism during anodic dissolution in the limiting current plateau. The experimental results indicate that increases in the amino acid concentrations inhibit the EP by 83.33% in the case of L-Methionine at 298 K. Density Functional Theory (DFT) was used to optimize geometry in the gas and liquid phases. The inhibition efficiency was associated with global reactivity descriptors, Fukui indices and dual local descriptors. The inhibitory performance is in good agreement with the estimated quantum chemical parameters, according to our findings.

The Maillard Reaction as Source of Meat Flavor Compounds in Dry Cured Meat Model Systems under Mild Temperature Conditions

Flavor is amongst the major personal satisfaction indicators for meat products. The aroma of dry cured meat products is generated under specific conditions such as long ripening periods and mild temperatures. In these conditions, the contribution of Maillard reactions to the generation of the dry cured flavor is unknown. The main purpose of this study was to examine mild curing conditions such as temperature, pH and aw for the generation of volatile compounds responsible for the cured meat aroma in model systems simulating dry fermented sausages. The different conditions were tested in model systems resembling dry fermented sausages at different stages of production. Three conditions of model system, labeled initial (I), 1st drying (1D) and 2nd drying (2D) and containing different concentrations of amino acid and curing additives, as well as different pH and aw values, were incubated at different temperatures. Changes in the profile of the volatile compounds were investigated by solid phase microextraction and gas chromatography mass spectrometry (SPME-GS-MS) as well as the amino acid content. Seventeen volatile compounds were identified and quantified in the model systems. A significant production of branched chain volatile compounds, sulfur, furans, pyrazines and heterocyclic volatile compounds were detected in the model systems. At the drying stages, temperature was the main factor affecting volatile production, followed by amino acid concentration and aw. This research demonstrates that at the mild curing conditions used to produce dry cured meat product volatile compounds are generated via the Maillard reaction from free amino acids. Moreover, in these conditions aw plays an important role promoting formation of flavor compounds.

Analysis of amino acids and acylcarnitines profiles in small, appropriate, and large for gestational age neonates

Objective To identify the amino acid (AA) and acylcarnitines (ACs) profiles in dry blood spot (DBS) of small, appropriate and large for gestational age neonates, and to investigate the difference AA and AC profiles in groups. Methods Eight thousand nine hundred and fourteen healthy singleton newborns were divided into three groups: small for gestational age (SGA) (n = 713), appropriate for gestational age (AGA) (n = 7866), and LGA (n = 335). Electrospray ionization tandem mass spectrometry was taken to evaluate the concentrations of 11 AAs and 31 ACs in DBS specimens obtained from all enrolled neonates. Results Compared with the AGA neonates, except propionylcarnitine, the concentrations of SGA neonatal most AAs and AC in the newborn were higher, while those of the newborns in the LGA group were lower. Conclusion Different concentrations of AAs and ACs in the three groups suggest that SGA may be associated with under anabolic metabolism, while LGA may be associated with over anabolic metabolism.

THE EFFECT OF FOLIAR APPLICATION OF AMINO ACIDS ON THEIR CONTENT AND COMPOSITION IN WINTER WHEAT GRAIN

Field experiments were carried out at the Experimental Station of Vytautas Magnus University (54º52′ N, 23º49′ E) in Lithuania. Winter wheat was grown on the background of N150P90K90 mineral fertilization and was additionally foliar-fertilized with different concentrations of amino acid solutions (0.5-3.0%) at stem elongation and heading stages. The study was aimed to establish protein content as well as quantitative and qualitative distribution of amino acids in winter wheat grain as influenced by fertilization with different concentrations of amino acids. Experimental evidence showed that solutions with different concentrations of amino acids applied at winter wheat stem elongation and heading stages increased grain protein content and total amino acids contents. Plants, fertilized with amino acids solutions at stem elongation stage accumulated higher protein content by on average 4.8%, at heading stage by 4.5% compared with the grain protein content of plants that had not received amino acids fertilization. The total amino acids content in winter wheat grain in response to amino acids applied at stem elongation stage increased by on average 2.0 g kg-1, at heading stage by 5.3 g kg-1. Nonessential and essential amino acids content in winter wheat grain was significantly increased by 2.5 and 3.0% amino acids solutions, applied at stem elongation stage by 0.5, 1.0, 1.5% amino acids solutions applied at heading stage. Winter wheat fertilization with amino acids solutions at stem elongation stage mostly increased the content of glutamic acid, at heading stage mostly increased the contents of glutamic, aspartic, valine, isoleucine, leucine, phenylalanine, methionine, threonine and lysine acids.

Amino acids regulate energy utilization through mammalian target of rapamycin complex 1 and adenosine monophosphate activated protein kinase pathway in porcine enterocytes.

As major fuels for the small intestinal mucosa, dietary amino acids (AA) are catabolized in the mitochondria and serve as sources of energy production. The present study was conducted to investigate AA metabolism that supply cell energy and the underlying signaling pathways in porcine enterocytes. Intestinal porcine epithelial cells (IPEC-J2) were treated with different concentrations of AA, inhibitor, or agonist of mammalian target of rapamycin complex 1 (mTORC1) and adenosine monophosphate activated protein kinase (AMPK), and mitochondrial respiration was monitored. The results showed that AA treatments resulted in enhanced mitochondrial respiration, increased intracellular content of pyruvic acid and lactic acid, and increased hormone-sensitive lipase mRNA expression. Meanwhile, decreased citrate synthase, isocitrate dehydrogenase alpha, and carnitine palmitoyltransferase 1 mRNA expression were also observed. We found that AA treatments increased the protein levels of phosphorylated mammalian target of rapamycin (p-mTOR), phosphorylated-p70 ribosomal protein S6 kinase, and phosphorylated-4E-binding protein 1. What is more, the protein levels of phosphorylated AMPK α (p-AMPKα) and nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylase sirtuin-1 (SIRT1) were decreased by AA treatments in a time depending manner. Mitochondrial bioenergetics and the production of tricarboxylic acid cycle intermediates were decreased upon inhibition of mTORC1 or AMPK. Moreover, AMPK activation could up-regulate the mRNA expressions of inhibitor of nuclear factor kappa-B kinase subunit beta (Ikbkβ), integrin-linked protein kinase (ILK), unconventional myosin-Ic (Myo1c), ribosomal protein S6 kinase beta-2 (RPS6Kβ2), and vascular endothelial growth factor (VEGF)-β, which are downstream effectors of mammalian target of rapamycin (mTOR). The mRNA expressions of phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform (PIK3CD) and 5′-AMP-activated protein kinase subunit gamma-1 (PRKAG1), which are upstream regulators of mTOR, were also up-regulated by AMPK activation. On the other hand, AMPK activation also down-regulated FK506-binding protein 1A (FKBP1A), serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform, phosphatase and tensin homolog (PTEN), and unc-51 like autophagy activating kinase 1 (Ulk1), which are up-stream regulators of mTORC1. Taken together, these data indicated that AA regulated cellular energy metabolism through mTOR and AMPK pathway in porcine enterocytes. These results demonstrated interactions of AMPK and mTORC1 pathways in AA catabolism and energy metabolism in intestinal mucosa cells of piglets, and also provided reference for using AA to remedy human intestinal diseases.

The effect of addition selected amino acids in extender semen on quality and DNA stability of frozen-thawed Sumba Ongole bull spermatozoa

<p class="abstrak1"><span lang="IN">The objective of the current study was to asses the optimal concentration of glutamine, glycine and cysteine amino acids in tris-citric-acid-fructose egg yolks (TCFY) extender on quality of SO bull spermatozoa during freezing and thawing. </span><span lang="EN-US">In t</span><span lang="IN">his study the DNA stability of frozen-thawed Sperm</span><span lang="EN-US"> was </span><span lang="IN">also indentified. Three mature bulls maintained at PT. Karya Anugerah Rumpin, private cattle breeding company, West Java, Indonesia were used as semen donors. Semen was collected using artificial vagina and were evaluated </span><span lang="EN-US">prior</span><span lang="IN"> to freezing. Semen was diluted with TCFY supplemented with different concentrations of amino acids (5, 15 and 25 mM glycine and glutamine, and 3, 5 and 7 mM cysteine) then processed for colling and freezing. Semen quality parameters (subjective motility, viability and membrane and DNA integrity). </span><span lang="EN-US">D</span><span lang="IN">ata showed that in general the effect of addition of selected amino acids (glycine, glutamine and cysteine) </span><span lang="EN-US">in</span><span lang="IN">to TCFY extenders on motility, viability and membrane integrity of SO spermatozoa after cooling were significantly different (p<0.05) higher than</span><span lang="EN-US"> that of</span><span lang="IN"> control. Addition of 15 mM glycine, 15 mM glutamine and 5 mM cysteine resulted in significant (p<0.05) increase post-thawing sperm motility and sperm viability as compared to th</span><span lang="EN-US">at of</span><span lang="IN"> control. Furthermore, when spermatozoa were stained with acridine orange after fixation with acetic alcohol, the DNA integrity of post-thawing spermatozoa showed that all spermatozoa were remain intact. In conclusion </span><span lang="EN-US">,</span><span lang="IN">addition of 15 mM glycine, glutamine and 5 mM cysteine increase the cryoprotecting efficacy of bovine bull cryopreservation extender, and furthermore all DNA spermatozoa were remain intact. </span></p>

Precipitation limits in pediatric parenteral nutritions with organic sources of calcium and phosphate

to determine if precipitation processes occur in parenteral nutrition solutions (PNs) with calcium gluconate and sodium glycerophosphate in the precipitation threshold limits of the Spanish SENPE/SEGHNP/SEFH 2008 consensus document of PN preparation. seven PNs with different composition were prepared in triplicate: five 100 ml PNs with different concentrations of amino acids, calcium and phosphorus similar to consensus document maximum concentrations for precipitation, and two control PNs: one without calcium and phosphorus and other with high calcium and phosphorus content and low concentration of amino acids. All PNs did not contain lipids to allow correct detection of precipitates. The no lipid PNs were stored at room temperature for 20 hours, and at 35 °C for four hours. Subsequently, they filtered through a 0.2 μm filter, which was observed by electron microscopy. Because a large amount of not expected precipitates was observed, complementary studies were carried out. precipitates were observed in all PNs except in the control solution without calcium and phosphorus; many of them were greater than 10 μm. However, according to our studies, these crystals were produced after filtration and calcium was found in their composition, but not phosphorus. Particles from the preparation of parenteral nutrition were also observed. in our study we did not find calcium phosphate precipitates in the limits included in the consensus document SENPE/SEGHNP/ SEFH. However, it is possible that micro precipitates with calcium are formed. It is important to filter PNs prior to their administration.

Effect of pre-harvest foliar application of amino acids and seaweed (Ascophylum nodosum) extract on growth, yield, and storage life of different bell pepper (Capsicum annuum L.) cultivars grown under hydroponic conditions

The study was set up to evaluate the efficiency of amino acids and seaweed on vegetative growth, reproductive phase, yield, and postharvest storage quality of hydroponically grown bell pepper cultivars “Sven Rz F-1” and “Red Knight.” Different concentrations of amino acid and seaweed were sprayed on bell pepper plants under hydroponic conditions. Pre-harvest foliar application of amino acids and seaweed significantly increased plant height, number of leaves, leaf area, yield per plant, number of marketable fruits per plant, average fruit diameter, fruit wall thickness, and average single fruit weight. Amino acid and seaweed significantly improved postharvest physicochemical quality of both bell pepper cultivars under extended cold storage conditions. Conclusively, amino acid as well as seaweed extract not only improved growth, development, yield, and overall quality of bell pepper cultivars but also increased storage life with reduced weight loss and decay along with better physicochemical quality.

Glutamic Acid as Corrosion Inhibitor for Aluminium in Acidic Medium

Corrosion behavior of aluminium in 0.6 mol. dm-3 NaCL solution in acidic medium ( ) was investigated in the absence and presence of different concentrations of amino acid, glutamic acid, as environmentally – friendly corrosion inhibitor over temperature range (293-308)K. The investigation involved electrochemical polarization method using potentiostatic technique and optical microscopy, the inhibition efficiency increased with an increase in inhibitor concentration but decreased with increase in temperature. Results showed that the inhibition occurs through adsorption of the inhibitor molecules on the metal surface and it was found to obey Langmuir adsorption isotherm. Some thermodynamic parameters ( ) and activation energy ( )were calculated to elaborate the mechanism of corrosion inhibition. The polarization measurements indicated that glutamic acid is of mixed type. The surface characteristic of the inhibited and uninhibited metal samples were investigated by optical microscopy.

Preparation of Non-Dairy Soft Ice Milk with Soy Milk

This study examined the influence of different ratios of soy milk replacement of skim milk (the levels of 0:100, 25:75, 50:50, 75:25 and 100:0 soy milk to skim milk) on the chemical, physical, microbiological and sensory properties of soft ice milk. The results revealed that by increasing the replacement of soy milk the total solids, fat, protein, pH, overrun and viscosity of mixture increased while acidity and melt down decreased significantly (P<0.05) in all treatments. Addition of soy milk in soft ice milk treatments has no significant effect on the counts of total viable bacterial and yeasts and molds. Adding cocoa (1%) in the treatment completely removes the beany flavour of sensory evaluation. Also, soy milk replacement up to 50% has no significant effect on sensory properties. It was found that soft ice milk of SIM4 sample contain greater amount of Na, Ca and Fe while the lowest value of Na, Ca and Fe was recorded in SIM0 sample. The content of isoflavones as daidzein can be varied from 230.40 to 105.13 μg, 100 ml-1 while isoflavones as genistein range from 20 to 4.98 μg, 100 ml-1 for soft ice milk samples. The total phenols and antioxidant contents increased of mixture by increasing of soy milk. Soft ice milk samples contained different concentrations of amino acids. Finally, the best ratio of soft ice milk samples is 50:50 ratio of soy milk to skim milk

Improvement of Phenols Production by Amino Acids in Callus Cultures of &amp;lt;i&amp;gt;Verbascum thapsus&amp;lt;/i&amp;gt; L.

A great mullein (Verbascum thapsus L.) was a famous species in Scrophulariaceae family. It was generally used as herbal medicine. Explants of V. thapsus (leaves and petioles) were cultured in vitro on Murashige and Skoog (MS) medium for shoot proliferation.Plantlet explants were cultured on MS medium supplemented with combination of Benzyl adenine (BA) and Naphthalene acetic acid (NAA) for callus induction. The best fresh and dry weight of callus formation was achieved using 0.5 mg/l BA. Quantitative analyses with High-performance liquid chromatography (HPLC) showed the content of phenols like Coumarin, Eugenol and Thymol were relatively low in leaves of mother plant, (10, 41, 310 ppm) respectively. The addition of different concentrations of amino acids as a precursor adding separately to the tissue culture medium led to raise the accumulation levels of phenolic compounds in callus tissue. Generally, the enhancement of accumulation depended on the type of amino acids and their concentration. The results showed 150 mg/l of Proline encouraged production of Comarin to 2752%, while 50 mg/l of Proline promoted accumulation of Eugenol to 290%. Whilst 150 mg/l of Tryptophan increased production of Thymol to 390%, in comparison with mother plant.

The effect of amino acids on nitrogen, phosphorus and potassium changes in spring barley under the conditions of water deficit

Small plot experiments were conducted on an Endocalcaric Endohypogleyic Luvisol in Aleksandras Stulginskis University to determine the effect of different concentrations of amino acids on the dynamics of nitrogen, phosphorus and potassium in spring barley leaves and on grain productivity under the water deficit conditions. The following experimental design was used: factor A − different concentrations of amino acid solutions: the control (unsprayed), sprayed with water, sprayed with 0.5, 1.0, 1.5, 1.5 % with microelements and 2.0% amino acid solutions; factor B – time of fertilization with the amino acids: BBCH 21–23 and 26–29 growth stages. Topsoil moisture content decreased from 14.5% to 8.7% during the BBCH 21–49 stages and thus it was considered as water deficiency conditions. The amino acid solutions of different concentrations, used for spring barley fertilization under the water deficiency conditions during the BBCH 21–23 or 26–29 stages, significantly increased the nitrogen, phosphorus and potassium content in the plants during the BBCH 26–29 and 32–35 stages in comparison to the control plants that were not sprayed with water. In many cases, the water spray stimulated nitrogen uptake to the same extent as amino acids application. Having analysed the effect of spring barley fertilization with the amino acids, it was found that the content of nitrogen did not reach the optimal level in the leaves of plants. However, the content of phosphorus reached the minimal limit of the optimal content at the BBCH 26–29 stage. A significant yield increase (0.15–0.47 t ha-1) was obtained when the spring barley had been fertilized with the 1.0% concentration of amino acids solution during the BBCH 21–23 stage.

NATURE'S STARSHIPS. I. OBSERVED ABUNDANCES AND RELATIVE FREQUENCIES OF AMINO ACIDS IN METEORITES

The class of meteorites called carbonaceous chondrites are examples of material from the solar system which have been relatively unchanged from the time of their initial formation. These meteorites have been classified according to the temperatures and physical conditions of their parent planetesimals. We collate available data on amino acid abundance in these meteorites and plot the concentrations of different amino acids for each meteorite within various meteorite subclasses. We plot average concentrations for various amino acids across meteorites separated by subclass and petrologic type. We see a predominance in the abundance and variety of amino acids in CM2 and CR2 meteorites. The range in temperature corresponding to these subclasses indicates high degrees of aqueous alteration, suggesting aqueous synthesis of amino acids. Within the CM2 and CR2 subclasses, we identify trends in relative frequencies of amino acids to investigate how common amino acids are as a function of their chemical complexity. These two trends (total abundance and relative frequencies) can be used to constrain formation parameters of amino acids within planetesimals. Our organization of the data supports an onion shell model for the temperature structure of planetesimals. The least altered meteorites (type 3) and their amino acids originated near cooler surface regions. The most active amino acid synthesis likely took place at intermediate depths (type 2). The most altered materials (type 1) originated furthest toward parent body cores. This region is likely too hot to either favor amino acid synthesis or for amino acids to be retained after synthesis.