Abstract
<p class="abstrak1"><span lang="IN">The objective of the current study was to asses the optimal concentration of glutamine, glycine and cysteine amino acids in tris-citric-acid-fructose egg yolks (TCFY) extender on quality of SO bull spermatozoa during freezing and thawing. </span><span lang="EN-US">In t</span><span lang="IN">his study the DNA stability of frozen-thawed Sperm</span><span lang="EN-US"> was </span><span lang="IN">also indentified. Three mature bulls maintained at PT. Karya Anugerah Rumpin, private cattle breeding company, West Java, Indonesia were used as semen donors. Semen was collected using artificial vagina and were evaluated </span><span lang="EN-US">prior</span><span lang="IN"> to freezing. Semen was diluted with TCFY supplemented with different concentrations of amino acids (5, 15 and 25 mM glycine and glutamine, and 3, 5 and 7 mM cysteine) then processed for colling and freezing. Semen quality parameters (subjective motility, viability and membrane and DNA integrity). </span><span lang="EN-US">D</span><span lang="IN">ata showed that in general the effect of addition of selected amino acids (glycine, glutamine and cysteine) </span><span lang="EN-US">in</span><span lang="IN">to TCFY extenders on motility, viability and membrane integrity of SO spermatozoa after cooling were significantly different (p&lt;0.05) higher than</span><span lang="EN-US"> that of</span><span lang="IN"> control. Addition of 15 mM glycine, 15 mM glutamine and 5 mM cysteine resulted in significant (p&lt;0.05) increase post-thawing sperm motility and sperm viability as compared to th</span><span lang="EN-US">at of</span><span lang="IN"> control. Furthermore, when spermatozoa were stained with acridine orange after fixation with acetic alcohol, the DNA integrity of post-thawing spermatozoa showed that all spermatozoa were remain intact. In conclusion </span><span lang="EN-US">,</span><span lang="IN">addition of 15 mM glycine, glutamine and 5 mM cysteine increase the cryoprotecting efficacy of bovine bull cryopreservation extender, and furthermore all DNA spermatozoa were remain intact. </span></p>
Highlights
Sumba ongole (SO) (Bos indicus) cattle is one of Indonesia superior local cattle that plays an important role in meat production for society
It is well known that mammalian spermatozoa contain high concentrations of polyunsaturated fatty acids, and are highly vulnerable to oxidative stress which is responsible for the generation of reactive oxygen species (ROS)
The main objective of the current study was to asses the optimal concentration of glutamine, glycine and cysteine amino acids in tris-citric-acid-fructose egg yolks (TCFY) extender on spermatozoa quality of SO
Summary
Sumba ongole (SO) (Bos indicus) cattle is one of Indonesia superior local cattle that plays an important role in meat production for society. It is necessary to improve the population and genetic quality of SO cattle by sperm cryopreservation and artificial insemination (AI). Cryopreservation of bull semen is still challenging due to lower fertility when compared to fresh semen because the all processes of cryopreservation including cooling, freezing and thawing create oxidative stress on the sperm membrane (Chatterjee et al 2001). It is well known that mammalian spermatozoa contain high concentrations of polyunsaturated fatty acids, and are highly vulnerable to oxidative stress which is responsible for the generation of reactive oxygen species (ROS)
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