Concentrations Of Chlorothalonil Research Articles

Individual and Mixture Toxicity of Three Pesticides; Atrazine, Chlorpyrifos, and Chlorothalonil to the Marine Phytoplankton Species Dunaliella tertiolecta

This study analyzed the toxicity of three pesticides (the herbicide atrazine, the insecticide chlorpyrifos and the fungicide chlorothalonil) individually, and in two mixtures (atrazine and chlorpyrifos; atrazine and chlorothalonil) to the marine phytoplankton species Dunaliella tertiolecta (Chlorophyta). A standard 96 h static algal bioassay was used to determine pesticide effects on the population growth rate of D. tertiolecta. Mixture toxicity was assessed using the additive index approach. Atrazine and chlorothalonil concentrations ≥ 25 µg/L and 33.3 µg/L, respectively, caused significant decreases in D. tertiolecta population growth rate. At much higher concentrations (≥ 400 µg/L) chlorpyrifos also elicited a significant effect on D. tertiolecta population growth rate, but toxicity would not be expected at typical environmental concentrations. The population growth rate EC50 values determined for D. tertiolecta were 64 µg/L for chlorothalonil, 69 µg/L for atrazine, and 769 µg/L for chlorpyrifos. Atrazine and chlorpyrifos in mixture displayed additive toxicity, whereas atrazine and chlorothalonil in mixture had a synergistic effect. The toxicity of atrazine and chlorothalonil combined was approximately 2 times greater than that of the individual chemicals. Therefore, decreases in phytoplankton populations resulting from pesticide exposure could occur at lower than expected concentrations in aquatic systems where atrazine and chlorothalonil are present in mixture. Detrimental effects on phytoplankton population growth rate could impact nutrient cycling rates and food availability to higher trophic levels. Characterizing the toxicity of chemical mixtures likely to be encountered in the environment may benefit the pesticide registration and regulation process.

Chlorpyrifos 및 Chlorothalonil의 사과 생산단계별 잔류특성

사과의 생산단계에서의 잔류허용기준 설정과 최종 소비단계에서의 안전성 평가자료로 활용하기 위하여 사과에 많이 살포되는 살충제 chlorpyrifos 및 살균제 chlorothalonil의 사과 재배기간 중 생물학적 반감기, 보관방법에 따른 잔류량 변화 그리고 부리별 잔류량 분포를 조사하였다. 사과 재배단계에서의 생물학적반감기는 chlorpyrifos 살포 후 15일 chlorothalonil 살포 후 30일까지의 잔류량 변화를 조사한 결과 chlorpyrifos 9.3일, chlorothalonil 32.2일로 나타났다. 사과의 유통과정중에서 일어날 수 있는 두 약제의 잔류량 변화는 실온저장시 chlorpyrifos 35일, chlorothalonil 56.3일이었고, 냉장저장시에는 chlorpyrifos 120.7일, chlorothalonil 182.2일의 반감기를 나타내었다. 사과의 부의별 잔류량을 조사한 결과 chlorpyrifos의 경우 움푹 파인 부위의 과피 77.1%, 나머지 과피 22.8%, 과육 부리에서는 0.1%로 나타났고, chlorothalonil의 경우 움푹 파인 부위의 과피 85.2%, 나머지 과피 10.4%, 과육 부위에서 4.4%로 나타났다. 사과 중 두 약제의 잔류량은 대부분인 <TEX>$95{\sim}99%$</TEX>가 과피에 잔류하는 것으로 나타났다. An organophosphorus insecticide, chlorpyrifos and an arylnitrile fungicide, chlorothalonil commonly used far apple were subjected to a residual investigation under field conditions to ensure safety of terminal residues at harvest. After pesticides were applied at standard rate in apple tree fer 15 days for chlorpyrifos and 30 days far chlorothalonil, persistence of their residues in apple was investigated by several interval. At harvest, residual concentrations of chlorpyrifos and chlorothalonil in apple were 1.3 and 2.4mg/kg, respectively, and the residue levels were higher than MRL 1.0 mg/kg in Korea. As well fitted by the first-oder kinetics, biological half-lives of the pesticide residues in apple were 9.3 days for chlorpyrifos and 32.2 days for chlorothalonil. During the storage, half-lives of chlorpyrifos and chlorothalonil were 35.0 and 56.3days at room temperature, and 120.7 and 182.8 days at 412, respectively. Distribution of chlorpyrifos residue in flesh, fruit skin and stalk cavity of each apple corresponded to 0.1% 22.8% and 77.1%, respectively. In case of chlorothalonil, residue in flesh, fruit skin and stalk cavity was 4.4%, 10.4% and 85.2%, respectively.

Risk assessment of pesticide runoff from turf.

The TurfPQ model was used to simulate the runoff of 15 pesticides commonly applied to creeping bentgrass (Agrostis stolonifera L.) fairways and greens on golf courses in the northeastern USA. Simulations produced 100-yr daily records of water runoff, pesticide runoff, and pesticide concentration in runoff for three locations: Boston, MA, Philadelphia, PA, and Rochester, NY. Results were summarized as annual and monthly means and annual maximum daily loads (AMDLs) corresponding to 10- and 20-yr return periods. Mean annual pesticide runoff loads did not exceed 3% of annual applications for any pesticide or site, and most losses were substantially less than 1% of application. However, annual or monthly mean concentrations of chlorothalonil, iprodione, and PCNB in fairway runoff often exceeded concentrations that result in 50% mortality of the affected species (LC50) for aquatic organisms. Concentrations of azoxystrobin, bensulide, cyfluthrin, and trichlorfon in extreme (1 in 10 yr or 1 in 20 yr) events often approached or exceeded LC50 levels. Concentrations of halofenozide, mancozeb, MCPP, oxadiazon, propiconazole, thiophanate-methyl, triadimefon, and trinexapac-ethyl were well below LC50 levels, and turf runoff of these chemicals does not appear to be hazardous to aquatic life in surface waters.

Risk Assessment of Pesticide Runoff from Turf

The TurfPQ model was used to simulate the runoff of 15 pesticides commonly applied to creeping bentgrass (Agrostis stolonifera L.) fairways and greens on golf courses in the northeastern USA. Simulations produced 100-yr daily records of water runoff, pesticide runoff, and pesticide concentration in runoff for three locations: Boston, MA, Philadelphia, PA, and Rochester, NY. Results were summarized as annual and monthly means and annual maximum daily loads (AMDLs) corresponding to 10- and 20-yr return periods. Mean annual pesticide runoff loads did not exceed 3% of annual applications for any pesticide or site, and most losses were substantially less than 1% of application. However, annual or monthly mean concentrations of chlorothalonil, iprodione, and PCNB in fairway runoff often exceeded concentrations that result in 50% mortality of the affected species (LC50) for aquatic organisms. Concentrations of azoxystrobin, bensulide, cyfluthrin, and trichlorfon in extreme (1 in 10 yr or 1 in 20 yr) events often approached or exceeded LC50 levels. Concentrations of halofenozide, mancozeb, MCPP, oxadiazon, propiconazole, thiophanate-methyl, triadimefon, and trinexapac-ethyl were well below LC50 levels, and turf runoff of these chemicals does not appear to be hazardous to aquatic life in surface waters.

Leaching of propiconazole and chlorothalonil during production of pinus sylvestris seedlings in containers

The risk of environmental contamination by pesticides is not well known in container production of forest seedlings. Leaching of propiconazole (Tilt 250 EC®) and chlorothalonil (Bravo 500®) from peat container medium into the ground was monitored during three growing seasons in nursery production of Scots pine (Pinus sylvestris L.) seedlings. Fungicides were applied at about 20 day intervals from the end of July until November. The annual load of leached propiconazole (25–183 g active ingredients ha‐1) was greater than that of chlorothalonil (5 to 82 g active ingredients ha‐1). The proportion of leached to applied propiconazole was large, 4–29%, but less than 1% of the applied chlorothalonil was detected in leachates. The downstream percolation of water in the soil beneath the container area was small. After extra irrigation into the ground, the detected concentrations of chlorothalonil in soil water 0.5 m beneath the ground surface were 0.4–2.4 μg 1‐1.

Leaching of Propiconazole and Chlorothalonil During Production of Pinus sylvestris Seedlings in Containers

The risk of environmental contamination by pesticides is not well known in container production of forest seedlings. Leaching of propiconazole (Tilt 250 EC®) and chlorothalonil (Bravo 500®) from peat container medium into the ground was monitored during three growing seasons in nursery production of Scots pine (Pinus sylvestris L.) seedlings. Fungicides were applied at about 20 day intervals from the end of July until November. The annual load of leached propiconazole (25–183 g active ingredients ha‐1) was greater than that of chlorothalonil (5 to 82 g active ingredients ha‐1). The proportion of leached to applied propiconazole was large, 4–29%, but less than 1% of the applied chlorothalonil was detected in leachates. The downstream percolation of water in the soil beneath the container area was small. After extra irrigation into the ground, the detected concentrations of chlorothalonil in soil water 0.5 m beneath the ground surface were 0.4–2.4 μg 1‐1.

The effect of fungicides on urediniospore germination and disease development of daylily rust

In response to the recent introduction and rapid spread throughout the United States of daylily rust caused by Puccinia hemerocallidis, research was conducted on the relative efficacy of seven fungicides (myclobutanil, propiconazole, flutolanil, triadimefon, chlorothalonil, mancozeb, and azoxystrobin) for control of this disease. Fungicides were applied at varying rates 24 h before spray–inoculation of daylilies ( Hemerocallis sp. cv. Pardon Me) with urediniospore suspensions. Disease development was evaluated by counting rust pustules on six to eight leaves per plant after 15 days. Nearly all fungicides reduced the number of pustules cm −1 of leaf length compared to the untreated control in all greenhouse trials. The protectant fungicides chlorothalonil and mancozeb significantly reduced pustule development. Of three sterol-biosynthesis inhibiting compounds, triadimefon provided greatest reduction in pustule development. Myclobutanil and propiconazole did not affect in vitro urediniospore germination on fungicide-amended potato dextrose agar (PDA). All concentrations of azoxystrobin and chlorothalonil significantly reduced urediniospore germination. A 24 h exposure to PDA amended with azoxystrobin (1.0–100.0 μg a.i. ml −1), chlorothalonil (100 μg a.i. ml −1) or mancozeb (10.0 and 100.0 μg a.i. ml −1) eliminated subsequent urediniospore germination on non-amended PDA. The data indicate that daylily rust can be controlled under greenhouse conditions by applications of fungicides.

Comparison of Five Extraction Methods for Determination of Incurred and Added Pesticides in Dietary Composites

The National Exposure Research Laboratory of the U.S. Environmental Protection Agency conducts research to measure exposure of individuals to chemical pollutants through the diet. In support of this research, methods are being evaluated for the determination of pesticides in dietary composite samples. In the present study, Soxhlet, blender, microwave-assisted, pressurized fluid, and supercritical fluid extraction methods were compared for the determination of incurred and added pesticides in 4 dietary composites, which varied in fat and water content. Incurred pesticides were chlorothalonil, chlorpyrifos, DDE, dicloran, dieldrin, endosulfan I, malathion, cis- and trans-permethrin, and trifluralin. Added pesticides were alpha- and gamma-chlordane, hexachlorobenzene, and fonofos. Concentrations of the individual pesticides were between 0.2 and 20 ng/g composite. All 5 methods tested could extract pesticides from dietary composites. Most incurred pesticides were recovered from the dietary composites within the range of 59-140% of expected values. Recoveries of added pesticides were between 60 and 130%. Microwave-assisted extraction led to significantly higher concentrations of 7 pesticides. Blender extraction yielded significantly higher concentrations of chlorothalonil and fonofos. Water content was a significant factor in the recovery of chlorothalonil, and fat content was a significant factor in the recovery of fonofos. In designing an exposure study, the selection of the extraction method would be determined by number of samples to be extracted, analyte stability, and cost.

Fate and environmental impact of pesticides in plastic mulch production runoff: field and laboratory studies.

Concentrations of copper, azinphosmethyl, chlorothalonil, and endosulfan sulfate ranged from less than 1 to greater than 1000 microg/L in runoff from tomato plastic mulch production. When this runoff entered local creeks, the copper concentration was as high as 22 microg/L, which exceeded the measured larval clam LC50 values of 21 and 12 microg/L Cu at 96 and 192 h, respectively. A greenhouse scale investigation of copper and toxicity demonstrated that sedimentation reduced total copper concentration in runoff by 90%, although the dissolved copper concentration was unchanged, averaging 139 +/- 55 microg/L. When runoff was applied to marine mesocosms containing grass shrimp and mummichog fish, unsettled runoff produced the greatest mortality, although even settled runoff caused more mortality than that in the control mesocosm receiving runoff without added copper. Desorption of soil-sorbed copper occurred quickly in saline water and contributed to toxicity. Copper toxicity in runoff can be reduced, but not eliminated, by sedimentation.

Sorção-dessorção do fungicida clorotalonil em solos com diferentes teores de matéria orgânica

A cinética e a sorção de pesticidas em solos permitem predizer a velocidade com que esta reação atinge o equilíbrio e investigar os possíveis mecanismos envolvidos durante a reação. Portanto, esses processos são fundamentais para que se possa compreender o destino dos pesticidas no solo. Este trabalho teve por objetivo estudar a sorção e a cinética do fungicida clorotalonil em cinco solos com diferentes teores de matéria orgânica do estado de São Paulo: Neossolo Quartzarênico órtico (RQo), Latossolo Vermelho distroférrico (LVdf-1 e LVdf-2), Latossolo Vermelho perférrico (LVj) e Gleissolo (G). Nestes estudos, foi utilizado traçador radioativo, ou seja, 14C-clorotalonil, e a radioatividade foi detectada por espectrometria de cintilação líquida. Os ensaios foram realizados em sala climatizada (25±2ºC), em ambiente escuro. O ensaio de cinética constou de oito períodos de equilíbrio: 0; 0,5; 1,0; 2,0; 4,0; 8,0; 12,0 e 24,0h; sendo que nas amostras que atingiram equilíbrio (24h) foram realizados os testes de dessorção, em quatro etapas subseqüentes. Nos estudos de isotermas de sorção, as concentrações de clorotalonil empregadas situaram-se entre 0,05 e 0,76µgmL-1. O modelo matemático de Elovich foi ajustado aos resultados do estudo de cinética e o modelo de Freundlich foi ajustado aos resultados do estudo de isoterma de sorção. Observou-se elevada sorção de clorotalonil nos solos estudados, exceto no solo arenoso com baixo teor de matéria orgânica (RQo). A sorção do clorotalonil relacionou-se positivamente com a matéria orgânica do solo. A cinética de sorção desse fungicida envolveu duas fases, uma imediata, de maior relevância quantitativa, e outra mais lenta. Esses resultados mostram que uma pequena fração do clorotalonil aplicado ao solo estaria disponível para ser lixiviado ao lençol freático, mas atenção especial é necessária quando ele é aplicado em solos arenosos com baixo teor de matéria orgânica. Além do que, a fração do fungicida sorvida na fase lenta também pode ser facilmente dessorvida à solução do solo.

Detection of Chlorothalonil in Dew Water Following Aerial Spray Application and Its Role in the Control of Black Sigatoka in Banana.

A standardized bioassay measuring the growth inhibition of Aspergillus niger in vitro allowed the detection of small concentrations (0.1 to 20 μg/ml) of chlorothalonil present in dew water on both the adaxial and abaxial surface of banana leaves in a commercial plantation receiving aerial sprays. Chlorothalonil concentrations detected in dew water on the banana leaf surface were within the range of concentrations required to prevent Mycosphaerella fijiensis (causal agent of black Sigatoka) ascospore germination in laboratory bioassays. When 9-cm-diameter banana leaf disks inoculated with M. fijiensis ascospores were immersed for 4 h in water containing 0.1 to 0.6 μg of chlorothalonil per ml, ascospore germination was inhibited by 96.9%. The EC50 values for inhibition of ascospore germination were between 0.01 and 0.03 μg/ml for chlorothalonil and between 3.2 and 3.7 μg/ml for mancozeb. Following a 4-h exposure to chlorothalonil and mancozeb, and subsequent removal of the fungicides by a washing step, ascospores failed to germinate, indicating that both fungicides are fungicidal to M. fijiensis, not fungistatic. Recovery analysis of chlorothalonil spray droplet deposits and active ingredient on deposition cards in the field during aerial spray applications indicated that detectable fungicide deposition on the abaxial leaf surface occurs only when the banana leaf-target is vertical or nearly so. The significance of this observation in relation to the control of black Sigatoka with protectant fungicides is discussed.

Tank Mix Combinations of Propiconazole and Chlorothalonil for Control of Leaf Spot Diseases of Peanut

Abstract Field experiments were conducted in one location in 1993 and two locations in 1994 to determine the effects of propiconazole and chlorothalonil tank mix combinations on severity of early (Cercospora arachidicola) and late (Cercosporidium personatum) leaf spot diseases of peanut (Arachis hypogaea L.). In all tests, 10 treatments consisted of 0 and 63 g a.i./ha of propiconazole and 0, 0.315, 0.63, 0.945, and 1.26 kg a.i./ha of chlorothalonil arranged factorially. In 1993, final leaf spot intensity ratings decreased according to nonlinear quadratic functions of chlorothalonil concentrations applied with and without propiconazole. No improvement in leaf spot control was evident with the addition of more than 0.945 kg a.i./ha of chlorothalonil with 63 g a.i./ha of propiconazole. In 1994, conditions were more conducive for leaf spot development. At the Plains location, final leaf spot intensity ratings decreased according to non-linear quadratic functions of chlorothalonil concentrations alone. Leaf spot intensity ratings decreased linearly with increasing rates of chlorothalonil when applied with 63 g a.i./ha of propiconazole. At Tifton, final leaf spot intensity ratings decreased linearly with increasing rates of chlorothalonil with or without propiconazole. Leaf spot intensity ratings were lower on plants treated with tank mixes of chlorothalonil and propiconazole compared to those treated with chlorothalonil alone. Pod yields increased linearly or according to quadratic functions of rates of chlorothalonil with or without propiconazole in both years and all locations. Across all rates of chlorothalonil, yields were higher from plants treated with propiconazole than those treated with the respective rates of chlorothalonil alone.

Hydrolytic biodegradation of chlorothalonil in the soil and in cabbage crops

Broccoli and Chinese cabbage crops were treated at planting by pouring an emulsion of the fungicide chlorothalonil around the stem of the plant. During culture, chlorothalonil was biodegraded in soil into l,3‐dicarbamoyl‐2,4,5,6‐tetrachlorobenzene (compound 1), l,3‐dicyano‐4‐hydroxy‐2,5,6‐trichlorobenzene (compound 2), and l‐carbamoyl‐3‐cyano‐4‐hydroxy‐2,5,6‐trichlorobenzene (compound 3). Compounds 1 and 2 were the major metabolites in soil. In the harvested broccoli (in the flower) and Chinese cabbages (the leaves), the concentrations of chlorothalonil and of compounds 1 and 2 were lower respectively than 0.1, 0.1 and 0.5mg/kg fresh weight.