Calcium Chloride Concentration Research Articles

Transcriptional and metabolite analysis reveal a shift in fruit quality in response to calcium chloride treatment on "Kyoho" grapevine.

'Kyoho' grapevine (Vitis vinifera) treated by calcium ions solution has been proved as an effective treatment to extend grape quality during storage to reduce disease, but its molecular mechanism was not clear yet. In the current work, grape berries were treated with different concentration of Calcium chloride (CaCl2) solution, and their effects on antioxidant enzyme activity and transcriptome and metabolome in fruit were investigated. CaCl2 treatments reduced weight loss and inhibited the decrement of flesh firmness. 80mM CaCl2 significantly increased the activity of antioxidant enzymes POD, SOD and CAT, which was the optimum experimental concentration. The study showed that the expression level of heat shock transcription factor and UBX which involved in endoplasmic reticulum stress and degradation pathway increased significantly. Moreover, the corresponding metabolites, such as heat shock protein and organic acid, also increased significantly. The misfolded proteins are transported to the cytosol for degradation, so that the preservation ability of grape is improved.

Assessment of coaxial printability for extrusion-based bioprinting of alginate-based tubular constructs

Coaxial printing has emerged as a versatile technique of bioprinting for better capability in tissue engineering by allowing the injection of two materials having different mechanical and biochemical properties in two distinct layers. Thus, coaxial printing can enhance mechanical properties as well as biological activities in a coaxial structure. However, despite these advantages of coaxial printing, there was no experimental study regarding the evaluation of coaxial printability and shape fidelity for extrusion-based bioprinting. In this study, we quantitatively investigated coaxial printability and shape fidelity of the coaxial-printed scaffolds using alginate and Pluronic F-127 which are the most widely used biomaterials for bioprinting. With the optimal concentrations of calcium chloride for alginate, a 3D tubular structure with high shape fidelity could be directly fabricated using core-crosslinking method. The presented results suggest a methodological solution to improve the coaxial printability for various tissue engineering applications using coaxial bioprinting.

Factors Affecting Permeability Reduction of MICP for Fractured Rock

Microbial induced Calcite Precipitation (MICP) has shown great potential to reduce the permeability of fractured rock, but its permeability reduction characteristic affected by solution concentration and the geometric morphology of fracture rock is not presented. Then MICP grouting and seepage test device were designed to study the effect of factors including urea and calcium chloride concentration, fracture roughness and aperture on permeability reduction of MICP on fracture rock. During the test, the same total volume was injected into each sample with the same injection times. The results showed that MICP grouting reduced the Darcy permeability coefficient of fractured rock to 3-5×10-5 m/s by 4 orders of magnitude smaller than that of non-grouted fractured rock. And with increasing of fracture roughness, the urea and calcium chloride concentrations and decreasing of fracture aperture, MICP grouting ratio in fractured rocks increased and the Darcy permeability coefficient decreased. And the permeability reduction of MICP on fracture rock increased with decreasing of the four factors. The permeability coefficient and the permeability reduction were the most sensitive to urea concentration. The permeability reduction could be fitted well by power function consider the above factors. The effects of fracture aperture larger than 2.5 mm or roughness larger than 0.873 mm need further research. The research results can provide important guidance and reference significance for microbial grouting to improve fractured rock performance.

Shape‐Controlled Synthesis of Multicomponent‐Encapsulating Alginate Microparticles: Peanut‐, Spherical‐, and Disc‐Shaped Transformations

AbstractMulticomponent‐encapsulating alginate microparticles were prepared using an electrospray system. The resultant particles were subject to stirring (varied from 0–1000 rpm) and cross‐linking processes with various concentrations of calcium chloride (varied from 2–16 wt %). We determined that a balance should be maintained between the stirring rate (related to the shear force) and cross‐linking degree (related to the hardness of the particle) to reach the shape control. The shape of the particle can be varied from a peanut shape to a disc shape, with up to 95.43 %±1.84 % monodispersed shape distribution. A microparticle drug encapsulation efficiency of 98 % was achieved, and the cell toxicity effect of reduction of 20–40 % growth toward ovarian cancer cell lines SKOV‐3 and CP70 by using the disk‐shape microparticle‐treated group was achieved, which is similar to that of 1/3 freeform dosage treatment group. Hyperthermia can be incorporated and integrated with chemotherapeutic treatment by applying an alternating magnetic field to multicomponent‐encapsulated alginate microparticles to further enhance the efficacy of treatment. The results of this study indicate that alginate particles can be used as drug carriers for ovarian cancer treatment and potentially for treatment of intraperitoneal metastatic carcinoma via spraying these disk‐shaped microparticles for internal lining attachment.

Synthesis and evaluation of the efficiency of antibacterial hydrogel beads based on the sodium alginate–ferula gum for delayed release of quercetin

In this study, novel alginate–ferula gum-based hydrogel beads with an antibacterial property which is attributed to the ferula gum were prepared by the ionic interactions method. The hydrogel beads were optimized using the Taguchi experimental design; the effect of the amount of ferula gum, the concentration of calcium chloride, and the amount of sodium alginate were investigated on the time required to release 50% of quercetin (Quer) from the hydrogel beads. In summary, ferula gum, calcium chloride, and sodium alginate have impacts on the time required to release 50% of Quer from the hydrogel beads, respectively. Hydrogel beads morphology was determined by XRD, DSC, FE-SEM, and FTIR analyses. The FE-SEM images confirmed that the presence of ferula gum reduces the hydrogel beads pores which reduce drug release rate over a long period. The antibacterial activity of the obtained sample was tested against Staphylococcus aureus and Escherichia coli, the results exhibited the remarkable antibacterial activity of hydrogel beads containing ferula gum. The hydrogel beads water content was calculated to be 487.34%. Quer release was examined in a buffer medium (pH = 7.4) for 60 h. Quer release from the hydrogel beads was slow and controlled. The release curves were nicely fitted by the Higuchi equation and the release followed by diffusion phenomenon or Fickian’s laws of diffusion.

The investigation of microbial induced calcium carbonate precipitation for soil improvement

Weak and unstable soils can limit the building of new infrastructure. Current soil strengthening techniques such as chemical grouting have detrimental effects on the environment from greenhouse gas production, soil pH modification and groundwater contamination. Microbial-induced calcium carbonate precipitation (MICCP) is a technique that utilises the ability of bacteria to precipitate calcium carbonate, which can be used for a variety of applications including binding adjacent soil particles and filling the pore spaces of soils to increase their mechanical properties. A commonly used bacterium is Sporosarcina pasteurii. A range of factors influences MICCP which presents challenges with process optimisation. Some studies have made use of computational models to predict biocementation at a larger scale, however aspects of models are based on assumption of conditions instead of experimental data. An aim of this project is to investigate urease activity in S. pasteurii by comparing different growth media, growth stages, pH and temperatures. Ureolysis kinetics of S. pasteurii will be investigated at different urea and calcium chloride concentrations in liquid media. Finally, the biocementation of S. pasteurii in sand syringe setups will also be investigated to compare the effects of changing influencing factors such as growth stage and cell concentration of S. pasteurii, sand particle size, cementation media concentration, duration between cementation media applications and overall number of cementation treatments. Experimental work will be particularly focused towards gaps in the experimental data used in computational models, to help improve these models and bring MICCP biocementation closer to commercial use.

Physical, mechanical and transport properties of emulsified films based on alginate with soybean oil: Effects of soybean oil concentration, number of passes and degree of surface crosslinking

Edible films and coatings have been applied on fruits to improve their quality during growth and development as well as during postharvest storage. A response surface methodology was used to determine the effects of oil concentration, number of passes and calcium chloride concentration on the physical, mechanical and transport properties of oil-alginate composite films. Alginate-based films were developed using alginate (1.0% w/v), soybean oil (0.5%, 1.0% and 1.5% v/v) and Tween 80® (1% v/v). Coarse emulsions were passed 2, 4 and 6 times through a microfluidizer at 200 MPa. Calcium chloride solutions (0%, 1.5% and 3%) were then applied on the cast films to crosslink them. The oil particle sizes ranged from 360 to 445 nm, regardless of the emulsion composition and number of passes. The lowest water permeability value (1.8857E-10 g/msPa) was obtained for 1.5% oil concentration, 6 passes and no crosslinking. The porosity of the films at low oil concentrations increased with an increase in crosslinking level. In comparison, the porosity of the films at high oil concentrations decreased with an increase in crosslinking level. Emulsified films showed reduced strength and increased elongation with an increase in oil concentration. Films treated with calcium chloride showed lower strength due to greater effects from oil than calcium chloride. In conclusion, oil incorporation in films was the predominant factor in improved film properties, especially for water vapor permeability.

Pectin Based Edible Film for Cinnamaldehyde Delivery System: Effect of Calcium Chloride Content on the Physical Characteristics of the Films

The cinnamon essential oil has antibacterial characteristic and sensitive to light and oxygen [1]. Due to its benefits from cinnamaldehyde, this compound has already used for antibacterial agent injected in polymer film [2]. Films are usually made from natural polymers as their main materials such as pectin. This research observed the influence of calcium chloride addition (0; 0.01; 0.02; and 0.03 g/mL solution) in the physical characteristics of pectin based edible films using the immersion method. The results indicated that calcium chloride content did not effect significantly. On the other hand, increasing calcium chloride content increased the water sorption of phosphate buffer saline solution and 0.03 g/mL of calcium chloride offered the lowest water sorption. Addition of cinnamaldehyde affected the surface morphology of the film and gave the crystal structure on the surface.

A nondestructive contactless technique to assess the viscoelasticity of blood clots in real-time

There is a need for reliable and quantitative real-time assessment of blood properties to study and treat a broad spectrum of disorders and cardiovascular diseases as well as to test the efficacy of hemostatic agents. In this study, the real-time changes in viscoelastic/rheological properties of bovine whole blood during coagulation induced by different concentrations of calcium chloride (CaCl2; 15, 25, 35 and 45 mM) was investigated. For this purpose, a novel, contactless technique was used to accurately measure the clotting characteristics under controlled and sterile conditions. It was demonstrated that, increasing the calcium concentration from low values (i.e., 15 and 25 mM), led to shorter reaction time; however, a further increase in calcium concentration (i.e., 35 and 45 mM) favored longer reaction times. Additionally, increasing the CaCl2 concentration resulted in higher shear storage modulus (i.e., stiffer clots). These results were also comparable to those generated by thromboelastrograph, a clinically established technique, as well as a conventional rheometer, which quantitatively verified the high correlation of the shear storage modulus data. In sum, the non-destructive testing technique used in this study is reproducible and sensitive in measuring clot formation kinetics, which could be applied to assess the efficacy of hemostatic agents, and may also contribute to better diagnosing relevant circulatory system diseases and conditions.

Combination of mild heat and calcium chloride treatment on the texture and bioactive compounds of purple shallot

Purple shallot (Allium ascalonicum L.) is one of the most tropical agricultural crop widely used as a food seasoning. It is a highly commercial and important commodity by the food industry, not only based on its economic value but also the bioactive compounds. Pretreatment is widely used before processing of agro-products to improve the quality of these products. In this study, the influences of the pretreatment were conducted by the combination of mild temperature ranging from 45 to 55°C and different concentrations of calcium chloride (from 0.25 to 0.75%) on the quality of raw purple shallot used for further study (e.g. freeze-drying and pickling). The samples were analyzed for the firmness, bioactive compounds (total phenolic, total flavonoid and quercetin content) and antioxidant activity. The results showed that mild water temperature and calcium chloride concentration were influent on the firmness and bioactive compounds of raw material. The addition of calcium can enhance the beneficial action of mild heat treatments on sensory properties of purple shallot. The optimal conditions of the process were stabilized with the desired function, obtained at 50°C of mild hot water temperature dipping and 0.64% of calcium chloride concentration.

Obtaining artificial carnallites from magnesium chloride brines

The excess of magnesium chloride brines is formed during the polymineral ores processing in order to obtain potassium sulfate. One way to regenerate such brines is to produce artificial carnallite. It is necessary to purify these brines from sulfates for their further use as raw materials for the artificial carnallite production. In this work, the process of desulfurization of magnesium chloride brines with a solution of calcium chloride is studied. The temperature and the processing time, the magnesium chloride solutions concentration and the consumption of desulfurizing agent (calcium chloride) influence on the degree of magnesium chloride solutions purification from sulfate ions was determined. It has been established that almost all sulfate ions interact with calcium ion in 15 minutes and the desulfurization degree reaches 98,08 %. The increase in duration of the suspension mixing is necessary in order to establish equilibrium in the system and relieve the supersaturation in the solution. It has been shown that with increasing solutions saturation with MgCl2, the degree of the magnesium chloride brines purification from SO4 2– ion increases. Complete precipitation of calcium sulfate requires a certain excess concentration of calcium chloride.

Optimization of in vitro pollen germination and pollen viability tests for Castanea mollissima and Castanea henryi

The Castanea Miller (Fagaceae) family are commercially important trees in the word, grown for their nuts that are rich in starch and nutrients. The production of nuts is related to pollen fertility, but little is known about pollen viability and germination of the family. In this paper, we investigated the staining and in vitro germination traits of Chestunt (Castanea mollissima Blume) and Chinese Chinquapin (Castanea henryi (Skam) Rehder & Wilson), which were widely cultivated in China. We aimed to determine the optimal media for pollen germination and to develop a method to estimate viability using a series of correlation analyses based on staining and germination results. Pollen viability was assessed using 2,3,5-triphenyl tetrazolium chloride (TTC), 2,5-diphenyl monotetrazolium bromide (MTT), acetic carmine, benzidine-H2O2, blue ink dyeing, methylene blue, I2-KI, inorganic acid, and peroxide staining methods. For germination tests, different concentrations of sucrose, boric acid (H3BO3), calcium chloride (CaCl2), and gibberellin (GA3) were used. Benzidine-H2O2 and MTT dyes were determined to be suitable for the pollen viability test. The optimal media compositions for C. mollissima and C. henryi both had 5% sucrose, 0.01% H3BO3, and 0.005% GA3, but their amounts of CaCl2 varied at 0.01 and 0.005%, respectively. Assessments of staining methods found that MTT staining exhibited a linear relationship with pollen germination (***p < 0.001), which overestimated viability by an average of 1.4× for C. mollissima and an average of 1.7× for C. henryi, compared to in vitro germination rates. The proportion of dark staining by MTT was close to the germination rate. This study has identified optimal media and a method to estimate germination viability, which will improve Castanea Miller breeding programs.

Effect of Stiffness of Large Extravascular Hematomas on Their Susceptibility to Boiling Histotripsy Liquefaction in Vitro

Large intra-abdominal, retroperitoneal and intramuscular hematomas are common consequences of sharp and blunt trauma and post-surgical bleeds, and often threaten organ failure, compartment syndrome or spontaneous infection. Current therapy options include surgical evacuation and placement of indwelling drains that are not effective because of the viscosity of the organized hematoma. We have previously reported the feasibility of using boiling histotripsy (BH)—a pulsed high-intensity focused ultrasound method—for liquefaction of large volumes of freshly coagulated blood and subsequent fine-needle aspiration. The goal of this work was to evaluate the changes in stiffness of large coagulated blood volumes with aging and retraction in vitro, and to correlate these changes with the size of the BH void and, therefore, the susceptibility of the material to BH liquefaction. Large-volume (55–200 mL) whole-blood clots were fabricated in plastic molds from human and bovine blood, either by natural clotting or by recalcification of anticoagulated blood, with or without addition of thrombin. Retraction of the clots was achieved by incubation for 3 h, 3 d or 8 d. The shear modulus of the samples was measured with a custom-built indentometer and shear wave elasticity (SWE) imaging. Sizes of single liquefied lesions produced with a 1.5-MHz high-intensity focused ultrasound transducer within a 30-s standard BH exposure served as the metric for susceptibility of clot material to this treatment. Neither the shear moduli of naturally clotted human samples (0.52 ± 0.08 kPa), nor their degree of retraction (ratio of expelled fluid to original volume 50%–58%) depended on the length of incubation within 0–8 d, and were significantly lower than those of bovine samples (2.85 ± 0.17 kPa, retraction 5%–38%). In clots made from anticoagulated bovine blood, the variation of calcium chloride concentration within 5–40 mmol/L did not change the stiffness, whereas lower concentrations and the addition of thrombin resulted in significantly softer clots, similar to naturally clotted human samples. Within the achievable shear modulus range (0.4–1.6 kPa), the width of the BH-liquefied lesion was more affected by the changes in stiffness than the length of the lesion. In all cases, however, the lesions were larger compared with any soft tissue liquefied with the same BH parameters, indicating higher susceptibility of hematomas to BH damage. These results suggest that clotted bovine blood with added thrombin is an acceptable in vitro model of both acute and chronic human hematomas for assessing the efficiency of BH liquefaction strategies.

Formulation and Evaluation of Pseudoephedrine Hydrochloride Loaded Alginate Microbeads

Multiple unit dosage forms such as microbeads have increased acceptance because of added even spreading of the drug in the gastrointestinal tract, unvarying drug absorption, abridged local irritation and removal of undesirable intestinal retaining of polymeric material, when compared to non-disintegrating single unit dosage form. The purpose of the presented research is to develop microbeads of pseudoephedrine hydrochloride utilizing sodium alginate as the hydrophilic carrier in combination with HPMC as drug release modifier to lessen the dosing frequency and thereby advance the patient compliance. The microbeads were formulated by varying concentrations of HPMC and calcium chloride. The optimum formulation was chosen based upon in vitro drug release studies and further evaluated. The compatibility of drug-polymer was studied using FTIR analysis. The prepared formulation underwent evaluation for various parameters like drug entrapment, microbeads size, swelling index, mucoadhesive property and stability. No significant drug-polymer interactions were observed in compatibility studies and the formulation was found to be stable on 45 days storage. The formulations exhibited an extended drug release pattern which was the ultimate aim of the study. The microbeads represented good yield, high drug entrapment, low microbeads size and appropriate swelling property. The in vitro wash-off test indicated that the sodium alginate microbeads represent decent mucoadhesive properties. Henceforth, the formulated HPMC coated sodium alginate beads can be utilized as a substitute and cost-effective carrier for the oral controlled delivery of pseudoephedrine hydrochloride.&#x0D; Keywords: microbeads, pseudoephedrine hydrochloride, sodium alginate, drug release

Effect of Xylanase Immobilisation Conditions by Combination of Entrapment and Covalent Binding on Alginate Beads

The immobilisation of enzymes offer improvement in enzyme stability and characteristics as well as overcome the limitations of free enzyme systems for commercial purposes. In the current study, xylanase was immobilised using a combination technique of entrapment and covalent binding within and onto calcium alginate beads. The sodium alginate and calcium chloride (CaCl2) concentration used for the preparation of alginate beads which is the support matrix for xylanase immobilisation were fixed at 3% (w/v) and 0.3 M, respectively. The effect of immobilisation conditions (agitation rate, enzyme loading, and glutaraldehyde concentration) were studied using One-Factor-At-a-Time (OFAT) approach. The best condition for optimum immobilisation yield (83.93%) was found to be made up of the following parameter combination: agitation rate, 200 rpm; xylanase loading, 200 U; and glutaraldehyde concentration, 12% (w/w). The study shows the immobilisation conditions play a significant role towards the immobilisation yield of xylanase.

A study of the effect of calcium chloride (CaCl2) and pH on the flocculation ability of Saccharomyces cerevisiae (NCYC 1195)

The best fermentation ability of yeast-forming flock species is highly desirable in the bioethanol production process. Saccharomyces cerevisiae NCYC 1195 is known to have the ability to form flock which is influenced by the concentration of calcium chloride (CaCl2) and pH. This is because calcium ions can form bonds with mannose on the surface of the cell wall of S. cerevisiae, while the degree of acidity will affect the charges of specific protein on the cell wall. The ability of flocculation will precipitate S. cerevisiae cells so that they will not be mixed with fermentation medium. Thus, the separation process of cell and fermentation media can be done quickly and efficiently, without expensive centrifugation step. The aim of this study was to determine the effect of CaCl2 concentration and pH that used to determine the ability and stability of the flock of S. cerevisiae. Three concentration of CaCl2 (10-5, 10-7, 10-9 M) were used as well as three variations of pH (4,5,6). Culture was incubated at 30°C with 100 rpm agitation and analysed for flocculation ability every 4 hours during 24 hours. The stability of the flock was analysed every 24 hours during 30 days. The highest flock formation ability (73.15%) was obtained with the concentration of CaCl2 concentration 10-9 M and pH 5.

A simple, non-toxic method for separating seeds based on density, and its application in isolating Arabidopsis thaliana seed oil mutants.

PremiseSeed oil is an economically important trait in Brassica oilseed crops. A novel method was developed to isolate Arabidopsis thaliana seeds with altered oil content.Methods and ResultsIn A. thaliana, seed oil content is correlated with seed density, with high‐oil seeds being less dense than wild type and tending to float in solution, and low‐oil seeds being denser and tending to sink. In contrast to previous methods, which used toxic chemicals and density gradient centrifugation, different concentrations of calcium chloride (CaCl2) were employed to separate seeds without the need for centrifugation. The method was validated using known seed oil mutants, and 120,822 T‐DNA mutagenized A. thaliana lines were then screened for novel seed density phenotypes.ConclusionsA number of candidate mutants, as well as new alleles of two genes known to influence seed oil biosynthesis, were successfully isolated.

A Statistical Study on the Development of Metronidazole-Chitosan-Alginate Nanocomposite Formulation Using the Full Factorial Design.

The goal of this study was to develop and statistically optimize the metronidazole (MET), chitosan (CS) and alginate (Alg) nanoparticles (NP) nanocomposites (MET-CS-AlgNPs) using a (21 × 31 × 21) × 3 = 36 full factorial design (FFD) to investigate the effect of chitosan and alginate polymer concentrations and calcium chloride (CaCl2) concentration ondrug loading efficiency(LE), particle size and zeta potential. The concentration of CS, Alg and CaCl2 were taken as independent variables, while drug loading, particle size and zeta potential were taken as dependent variables. The study showed that the loading efficiency and particle size depend on the CS, Alg and CaCl2 concentrations, whereas zeta potential depends only on the Alg and CaCl2 concentrations. The MET-CS-AlgNPs nanocomposites were characterized by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), scanning electron microscopy (SEM) and in vitro drug release studies. XRD datashowed that the crystalline properties of MET changed to an amorphous-like pattern when the nanocomposites were formed.The XRD pattern of MET-CS-AlgNPs showed reflections at 2θ = 14.2° and 22.1°, indicating that the formation of the nanocompositesprepared at the optimum conditions havea mean diameter of (165±20) nm, with a MET loading of (46.0 ± 2.1)% and a zeta potential of (−9.2 ± 0.5) mV.The FTIR data of MET-CS-AlgNPs showed some bands of MET, such as 3283, 1585 and 1413 cm−1, confirming the presence of the drug in the MET-CS-AlgNPs nanocomposites. The TGA for the optimized sample of MET-CS-AlgNPs showed a 70.2% weight loss compared to 55.3% for CS-AlgNPs, and the difference is due to the incorporation of MET in the CS-AlgNPs for the formation of MET-CS-AlgNPs nanocomposites. The release of MET from the nanocomposite showed sustained-release properties, indicating the presence of an interaction between MET and the polymer. The nanocomposite shows a smooth surface and spherical shape. The release profile of MET from its MET-CS-AlgNPs nanocomposites was found to be governed by the second kinetic model (R2 between 0.956–0.990) with more than 90% release during the first 50 h, which suggests that the release of the MET drug can be extended or prolonged via the nanocomposite formulation.

New alginate-based interpenetrating polymer networks for water treatment: A response surface methodology based optimization study

Different interpenetrating polymeric networks (IPN) based on sodium alginate, carrageenan and bentonite were developed to remove heavy metals and dyes from contaminated water. Four significant preparation factors; crosslinking time, calcium chloride concentration, alginate to carrageenan mass ratio,and bentonite to carrageenan mass ratio were studied and optimized via full factorial design and response surface methodology to determine the optimum composition with highest adsorption capacity. Different optimal conditions and combinations were found depending on the type of heavy metal or dye to be removed. Low calcium chloride concentration was a common factor in all cases of heavy metals and dyes removal which indicates the negative effect of excessive crosslinking on the removal percentage. The adsorption capacity of methylene blue, Fe3+, Ni2+, and Cr3+ ions is 1271, 1550, 1500 and 1540 mg/g adsorbent, respectively. Reusability tests confirmed that the optimized formulations can be reused five successive times without significant drop in their removal efficiency. Upon utilization of the optimized formulations on real contaminated waters from tannery plant and oasis groundwater, they demonstrated an excellent performance as they removed above 95% of the original heavy metals contaminants and 40% of the acidic dye content.

Development of natural fiber-supported high-strength autologous fibrin glue from human plasma

Commercially available fibrin glue is expensive and contains fibrin and thrombin from different sources. This limits the use of this glue for bonding regular wounds, increases the cost for cardiovascular surgeries and aggravates the risk of inflammatory and immune systemic allergic reactions. This study aims to develop a stronger and affordable fibrin glue synthesized from the patient’s own plasma within a few minutes. Fibrin glue was synthesized by combining calcium chloride with human plasma and a gel-like adhesive was prepared for direct application. Different properties and morphological features of the autologous glue along with the fiber-supported one were evaluated for different concentrations (5, 10 and 20% w/v) of calcium chloride. 10% calcium chloride was found as the optimum with clotting time varying from 8 to 25 min for frozen plasma and 3 min for freshly prepared plasma. The average adhesion strength was 46.2, 55.8 and 40.5 g/cm2 for 5, 10 and 20% calcium chloride, respectively. The adhesion strength increased up to 74.8 g/cm2 due to the addition of 1 mL of 0.5% cellulose that addresses the issue of weaker adhesion strength found in typical autologous glue. The average water content was the lowest for 10% calcium chloride and was reduced to 15.3% after cellulose addition. The proposed fibrin glue has high adhesion strength like the commercial ones and can be prepared in a few minutes. This affordable, enhanced fibrin glue has the potential to be used in a wide range of applications without the risk of possible inflammatory and allergic reactions.