Alpha-linolenic Acid Content Research Articles

Effect of Dietary Hemp Cake Inclusion on the In Vivo and Post Mortem Performances of Holstein Veal Calves.

Fifty-two male Holstein veal calves were divided into two homogeneous groups receiving two isoenergetic and isonitrogenous concentrates without (CTR group) or with 3% of hemp cake (HC group). The trial lasted for 171 days. All the calves were weighed five times during the trial. At slaughtering, carcasses were weighed and measured. Meat quality was determined on the Longissimus dorsi muscle. Average daily gain in the first period of the experiment (0-80 d) and dressing percentage and rump width of the carcasses were higher in HC group. Cooking weight losses and shear force were higher in the meat of the HC group while color parameters were similar in the two experimental groups. Unexpectedly, the alpha-linolenic acid content of meat was lower in the HC group. In conclusion, hemp cake can be considered an interesting ingredient in the concentrate used for the production of veal calves, but further studies will be needed to determine a suitable dosage in order to improve the nutritional quality of meat (i.e., the n-3 fatty acids content) without negative effects on physical characteristics.

Phytogenic Antioxidants Prolong n-3 Fatty Acid-Enriched Eggs’ Shelf Life by Activating the Nrf-2 Pathway through Phosphorylation of MAPK

Helpful for human health, omega-3 (n-3)-enriched eggs are preferred by consumers. However, antioxidants should be added to the hen’s diet to prevent n-3 fatty acid oxidation due to their unsaturated bonds. A study was designed to investigate the effects of different antioxidants on performance, egg quality, fatty acid profile, oxidation parameters, gene expression, and magnum morphology. A total of 450 hens were divided into five dietary groups. Wheat–flaxseed was used for the basic diet (control) and supplemented with vitamin E (VE), chlorogenic acid (CA), polyphenol (PF), and lutein (L). The experiment lasted for 10 weeks. The eggs were collected on the 5th week and were analyzed for quality, oxidative stability, and fatty acid (FA) content, being stored for 0 d, 7 d, 14 d, 21 d, 28 d, 35 d, and 42 d. The results showed that supplemental VE, PF, CA, and L improved the egg weight and hen day egg production compared to the control group (p < 0.05). The VE, PF, and L groups significantly (p < 0.05) reduced the malondialdehyde (MDA) and maintained the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in the egg yolk. The albumen height and Haugh unit were maintained in the egg yolk till 35 days of storage by the VE, PF, and L groups, while the CA group reduced the albumen quality after 21 d storage. The VE, PF, CA, and lutein maintained the content of alpha-linolenic acid (ALA), during the whole storage period. The total n-3 FA and docosahexaenoic acid (DHA) were retained in the egg yolk till 35 and 28 days of storage, respectively, and slightly decreased after 35 and 28 days in the L groups. The total n-6 (Tn-6) FA was maintained in the yolk till 28 days of storage in the CA and PF groups, respectively. The VE, PF, and L groups upregulated the expression of Nrf-2, P38MAPK, HO-1, SOD-1, and GSH-Px as compared to the CA and control groups. The VE, PF, and L groups significantly increased the magnum primary folds and epithelium height as compared to CA and the control. Thus, it was concluded that the use of PF and L is better at preventing egg quality deterioration and lipid oxidation, maintaining more than 300 mg/egg n-3 FA during storage, by activating the Nrf-2 pathway through the phosphorylation of P38MAPK, and enhancing the phase-2 antioxidant defense enzymes, namely, SOD, GSH-Px, and HO-1.

Natural Variation of Fatty Acid Desaturase Gene Affects Linolenic Acid Content and Starch Pasting Viscosity in Rice Grains.

Rice, as one of the main food crops, provides a vital source of dietary energy for over half the world’s population. The OsFAD3 gene encodes fatty acid desaturase, catalyzing the conversion of linoleic acid (LA) to alpha-linolenic acid (ALA) in rice. However, the genetic characterization of OsFAD3 and its role in the conversion of LA to ALA remains elusive. Here, we validated the effects of two homologous genes, OsFAD3-1 and OsFAD3-2, on the ALA and LA/ALA ratio in rice grains using near-isogenic lines. Two major haplotypes of OsFAD3-1 are identified with different effects on the ALA and LA/ALA ratio in rice germplasm. High expression of OsFAD3-1 is associated with high ALA accumulation and eating quality of rice grains. Overexpression of OsFAD3-1 driven by a seed-specific promoter increases the ALA content up to 16-fold in the endosperm. A diagnostic marker is designed based on an 8-bp insertion/deletion in the OsFAD3-1 promoter, which can recognize OsFAD3-1 alleles in rice. These results indicate that OsFAD3-1 is a useful target gene in marker-assisted breeding programs to improve varieties with high ALA and appropriate LA/ALA ratio in brown rice.

Carboxymethyl chitosan coated alpha-linolenic acid nanoliposomes: Preparation, stability and release in vitro and in vivo

Nanoliposome encapsulation combined with carboxymethyl chitosan (CMCS) surface decoration was employed to improve physicochemical stability and oral bioavailability of alpha-linolenic acid (ALA). Different nanoliposome systems including ALA-loaded nanoliposomes (ALA-NLs) and CMCS-coated ALA-NLs (CMCS-ALA-NLs) were characterized through dynamic light scattering, transmission electron microscope, Fourier transform infrared spectroscopy and differential scanning calorimetry. The results showed that CMCS-ALA-NLs had good encapsulation efficiency of 79% and layer formation with nanosized spherical carrier. The physicochemical stability of CMCS-ALA-NLs was better than that of ALA-NLs. CMCS-ALA-NLs were able to regulate the release of ALA in a simulated gastrointestinal environment. In vivo testing found that ALA concentration of CMCS-ALA-NLs had an area under the curve of 1.32, which was 1.28 times higher than that of ALA-NLs and 2 times higher than that of ALA-emulsion. The absorption of ALA was improved by CMCS-ALA-NLs. It suggested that CMCS-coated nanoliposomes should be an available delivery strategy for transporting ALA.

Quantitative relationships between ingested and intestinal flows of linoleic and alpha-linolenic acids, body weight and milk performance in mid-lactation dairy cows

Linoleic acid (LA) and alpha-linolenic acid (ALA) are essential fatty acids found in variable quantities in ruminant feedstuffs. Revision of French feed unit systems in 2018 has proposed the reassessment of energy requirements through a between-experiment approach expressing metabolisable energy supply as a function of the energy expenditures for maintenance and production, with these expenditures that reflect homeorhetic regulations. Based on the same approach, LA and ALA intake can be related to animal characteristics (i.e., BW) reflecting maintenance expenditures and secretion characteristics (i.e., milk yield, milk fat content and contents of LA and ALA in milk fat). Therefore, the objective of this work was to analyse the between-experiment relationships between ingested, duodenal, or absorbed flows of LA and ALA, BW and milk LA and ALA secretion by meta-analysis in mid-lactation dairy cows. These relationships were analysed using LA and ALA subsets of 96 and 99 experiments, respectively. Between-experiment regressions of daily flows of ingested, duodenal or absorbed LA and ALA on BW and milk LA and ALA flows were studied, with statistical unit defined as the mean of within-experiment treatments. For LA, the BW-associated coefficient was 0.019 (±0.0034) g absorbed LA/d per kg BW and milk LA secretion-associated coefficient was 0.70 (±0.081) g absorbed LA/g of LA secreted into milk. For ALA, the BW-associated coefficient was 0.0058 (±0.00093) g absorbed ALA/d per kg BW and milk ALA secretion-associated coefficient was 0.57 (±0.097) g absorbed ALA/g of ALA secreted into milk. When coding the diets as either control or milk fat depression diets, the BW-associated coefficient for LA was 0.017 (±0.0032) g absorbed LA/d per kg BW for both diets. For milk fat depression diets, milk LA secretion-associated coefficient was 1.02 (±0.119) g absorbed LA/g of LA secreted into milk, whereas it was 0.70 (±0.075) g absorbed LA/g of LA secreted into milk for control diets. Significant BW and milk performance coefficients were obtained in all LA and ALA equations, allowing the calculation of ingested and intestinal flows of LA and ALA based on measured BW, milk fat yield and milk fat content of LA and ALA. The relationships between ingested and intestinal flows of LA and ALA, BW and milk performance obtained in the present work could be integrated into renewed feed unit systems for energy and protein in dairy cows.

Influence of alpha linolenic acid on the motility, viability, antioxidant activity and fertility of frozen-thawed New Zealand white rabbit buck semen

Freezing and thawing processes result in production and accumulation of high concentrations of reactive oxygen species that are detrimental to spermatozoal motility and fertility. Therefore, supplementation of exogenous source of antioxidants to freezing diluent is crucial. The aim of the present study was to investigate for the first time whether supplementation of semen diluent with alpha linolenic acid (ALA) can improve motility, viability, membrane integrity, antioxidant status and fertility of post-thaw rabbit spermatozoa. Semen was collected and pooled from fifteen New Zealand white rabbit bucks. Semen samples were diluted with a tris-citrate-glucose (TCG) extender supplemented with ALA (0, 50, 75 and 100 μmol). Then, extended rabbit semen was cooled at 5°C and cryopreserved in liquid nitrogen. After thawing, spermatozoal quality parameters (individual motility %, viability %, osmotic resistance %, and acrosome integrity %), antioxidant activity (SOD, CAT, and GSH activities), lipid peroxidation (malondialdehyde) and fertility (conception and kindling rates) were evaluated. Results revealed that supplementation of rabbit semen extender with 50 μmol ALA significantly (P&lt;0.05) increased spermatozoal characteristics including motility (56.54%), viability (60.01%), acrosome status (72.66%) and membrane integrity (59.13%). The activity of semen antioxidant enzymes (SOD, CAT, and GSH) showed a significant improvement with a marked decrease in lipid peroxidation. Moreover, the conception (73.30%) and kindling (70.00%) rates were significantly (P&lt;0.05) higher in does inseminated with thawed semen treated with 50 μmol ALA in comparison with other concentrations (0, 75 and 100 μmol). In summary, supplementation of rabbit semen extender with 50 μmol ALA improved motility, viability, membrane integrity, acrosome integrity, antioxidant enzymes activity and fertility of post-thaw rabbit spermatozoa. Our findings suggested that higher concentrations of ALA are detrimental to post-thaw characteristics of New Zealand white rabbit buck spermatozoa. To achieve better results, the semen freezing extender should be supplemented with ALA at lower concentrations, especially 50 μmol.

Dietary Ground Flaxseed Increases Serum Alpha-Linolenic Acid Concentrations in Adult Cats.

Simple SummaryThe use of flaxseed in complete pet foods as a source of omega-3 fatty acids, and in particular alpha-linolenic acid, has been studied extensively in dogs. However, to date, no scientific published literature exists on the impacts of feeding whole ground flaxseed as an ingredient in cat food on nutrient digestibility, fatty acid bioavailability, and overall health in cats. In addition, dietary flaxseed inclusion has been challenged as providing little nutritional value for cats by not changing serum fatty acid levels and lowering nutrient digestibility. Therefore, the objective of this study was to directly evaluate the effects of adding ground flaxseed to dry cat food on fatty acid bioavailability, stool consistency, nutrient digestibility, and overall health in adult cats. These results clearly exhibit that a flaxseed-supplemented diet resulted in no changes in stool quality or nutrient digestibility, and elicited an increase in serum alpha-linolenic acid concentrations in healthy cats. Overall, these results clearly demonstrate that ground flaxseed can be used to modulate serum fatty acid concentrations and serve as a bioavailable source of alpha-linolenic acid in commercial cat food.We evaluated effects of dietary ground flaxseed on fecal and serum alpha-linolenic acid (ALA) concentrations, nutrient digestibility, and stool quality in female and male adult cats (n = 20 (8 males, 12 females); 3.95 ± 1.49 years of age (mean ± SD); 3.88 ± 0.82 kg BW). We hypothesized that adding ground flaxseed would increase serum ALA compared with feeding no flax, without changing nutrient digestibility. Cats were fed as-is 2.6% added-flaxseed (flax, n = 10) or no-flax (control, n = 10) diets (2.66 vs. 0.78% ALA of total fatty acids; crude protein 35%, fat 20%, fiber 3% as-fed) twice daily to maintain body weight for 28 days. Fecal collections were conducted on days 23–27 for total-tract nutrient digestibility, stool quality (scale 1–5; 1 = watery diarrhea, 5 = hard, dry, crumbly) and long-chain fatty acid (LCFA) analyses. Blood was collected on days 0, 14, and 28 for serum LCFA and chemistry analysis. Digestibility and fecal data were analyzed by ANOVA (SAS v9.4, Cary, NC, USA) and a repeated measures ANOVA for serum ALA. Flax-fed cats, compared with control-fed, had greater (p < 0.05) serum ALA after 14 days (4.00 vs. 0.71 µg/mL) and 28 days (7.83 and 3.67 µg/mL). No differences were observed in stool quality, and dry matter, protein, fat, and ALA digestibility. However, metabolizable energy was greater in the flax vs. control diet (4.18 vs. 3.91 kcal/g; p < 0.05). Overall, these data demonstrate that ground flaxseed added to cat diets increases serum ALA within 14 days, with no detriments to nutrient digestibility. We conclude that flaxseed can be used as a bioavailable source of ALA in cat diets.

Effect of extruded linseed on sarda donkey milk quality

The aim of this work was to evaluate the effect of extruded linseed in donkey diet on milk yield, milk composition and milk fatty acids (FA) profile. Eight Sarda donkeys were allocated to two groups homogenous for milk yield (0.354 ± 124 kg/d), days in milking (123 ± 41 d), and body weight (110 ± 15 kg). The first group was fed a control diet (CON), while the second group was fed the control diet supplemented with 100 g/day per head of extruded linseed (ELS). The trial lasted 7 weeks, with 2 weeks of adaptation and 5 weeks of experimentation. Data were analysed using a linear mixed model, considering the diet, sampling time and their interaction as fixed effects and animals as random effect. Milk yield and milk chemical composition were not affected by the diet. The diet led to some variations of FA profile. Long-chain FA, unsaturated FA and polyunsaturated FA were higher in ELS compared to control group. These results reflected the trend of some individual FA, in particular the concentration of alpha-linolenic acid. This FA was affected by diet, sampling time and their interaction and, at the end of the trial, the average value was double in ELS than CON. Dietary linseed reduced thrombogenic and atherogenic indices and increased hypo-to hypercholesterolemic ratio of milk. In conclusion, extruded linseed can be used in donkey diet to improve the milk FA profile, in terms of polyunsaturated FA and nutritional indices, which could have a positive impact on human health. Highlights The valorisation of donkey milk could be a good strategy to preserve donkey native breed. Extruded linseed, rich in alfa-linolenic acid, improves fatty acid profile of donkey milk, increasing PUFA and decreasing SFA. Linseed supplementation affects positively milk nutritional indices.

In Vivo Antitumoral Effects of Linseed Oil and Its Combination With Doxorubicin.

Linseed oil (LO) is known for its exceptional nutritional value due to the high content of alpha-linolenic acid (ALA), an essential omega-3 polyunsaturated fatty acid; its anticarcinogenic effect has been established in several experimental and epidemiological studies. As an adjuvant of chemotherapeutic agents, LO and other ALA-rich vegetable oils have been studied in only a handful of studies at the experimental level. However, the efficacy of antitumoral therapy using doxorubicin (Dox) in combination with ALA and ALA-rich substrates has not yet been investigated. In this work, the antitumor activity of LO in a wide dose range was studied with monotherapy and combined with Dox in animal models with Pliss lymphosarcoma (PLS) and Lewis lung adenocarcinoma (LLC). It was founded the daily oral administration of LO (1, 3, and 10 ml per 1 kg) to rats (PLS) and 6 ml/kg to mice (LLC) for 11–12 days from 7 days after subcutaneous transplantation of tumors has a stable statistically significant effect on the dynamics of tumor growth, reducing the intensity of tumor growth and increasing the frequency of complete tumor regressions (CR) compared with the control. LO showed high antimetastatic activity in the LLC model. Furthermore, LO at a dose of 3 ml/kg potentiates the antitumor effect of Dox in the PLS model, reducing the volume of tumors at the end of treatment by 2.0 times (p = 0.013), the value of the tumor growth index by 1.6 times (p < 0.03) and increasing the frequency of CR 60 days after the start of therapy by 3.5 times (p = 0.019) compared with the use of Dox alone. The combination of Dox and LO or fish oil allows growing efficiency therapy of LLC in comparison with Dox alone, increasing the frequency of CR to 73.68% and 94.4%, respectively, and reducing the frequency of metastasis to zero.

Effect of Concentration of Flaxseed (Linum usitatissimum) and Duration of Administration on Fatty Acid Profile, and Oxidative Stability of Pork Meat.

Simple SummaryDietary fat intake is substantially high in Western countries, resulting in overweight, obesity and cardiovascular diseases (CVD) among consumers. One way to reduce the incidence of CVD is to change food consumption and food intake with a higher content of polyunsaturated fatty acids (PUFA) in the human diet. Meat and meat products are considered to be the main source of dietary fats, especially as a source of saturated fatty acids. The aim of this study was to increase the proportion of PUFAs and, conversely, to decrease the proportion of saturated fatty acids in fat of pork meat. Flaxseed with a concentration of alpha-linolenic acid (ALA) at 57% was used in the swine diet. Flaxseed supplementation at two inclusion levels (5% and 10%) in two time intervals (3 and 6 weeks before slaughter) was evaluated. The aim was to increase the proportion of n-3 PUFAs, especially ALA, eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) and to improve the n-6/n-3 PUFA ratio in produced pork meat. The storage conditions and oxidation stability of the produced meat under refrigerator temperature (+4 °C) were also observed.Flaxseed is a common ingredient used for livestock feed. The aim of this work was to study the effect of a diet supplemented with flaxseed at 5% and 10% concentrations in the intervals of 3 and 6 weeks prior slaughter on fatty acid profile and oxidative stability of pork meat. Meat samples were collected after slaughter from each animal (five groups, n = 6). Samples of the musculus longissimus dorsi (MLD) and the musculus gluteobiceps (MGB) were selected. Chemical composition, fatty acid profile and oxidative stability during the storage of meat under chilling conditions (4 °C, 7 days) was analyzed. The addition of flaxseed significantly affected the composition of fatty acid profile and the shelf life of the produced meat. The fat content was changed in the experimental groups with 10% flaxseed supplementation (10.84% in MGB and 9.56% MLD) versus the control group. Despite the different concentrations of flaxseed, the best EPA/AA ratio was observed in the experimental groups fed with flaxseed supplementation for 3 weeks. The worst oxidative stability of meat samples (p < 0.05) was recorded in the experimental groups with the addition of flaxseed for 6 weeks, which was related to higher PUFA content in samples of the experimental groups and higher susceptibility of PUFAs to lipid oxidation. The oxidative stability of meat in the experimental group fed 5% flaxseed supplementation for 3 weeks was not affected.

Flaxseed and Seed Oil: Functional Food and Dietary Support for Health

Linum usitatissimum commercially known as Flax or flaxseed is mainly considered as an oilseed crop all over the globe belonging to the family Linaceae. Flax was valued in Ancient and Early Modern times as both food and medicine. Linseed has an important position in the Indian economy due to its wide industrial utility. In recent years, it emerged as a main key resource of phytochemicals in nutritional and functional aspects of human health. Moreover, due to other nutritional considerations than its oil content, make it a more favorable choice for food technologists and nutritionists to develop it as a functional food. Several studies divulge that these ingredients work well for nutritional advantage for human beings. Scientific evidence suggests and supports flaxseed consumption due to its quality proteins, soluble fibers, and a rich source of phenolic compounds. However, a large sector of the population all over the globe is still unaware of the health benefits supplementary to its consumption and its possible applications as useful food ingredients in food and food products. Flaxseed is mainly known for its high alpha-linolenic acid content, but it is also a rich source of lignan, compounds which are biologically active in the prevention of some chronic diseases. Flaxseed dietary fiber exhibits positive effects to reduce constipation, keep better bowel movement, and as a hypocholesterolemic agent. Over and above, numerous researchers reported that flaxseed incorporated food products can have good customer adequacy along with their nutritional welfares.

Brown and golden flaxseed reduce intestinal permeability and endotoxemia, and improve the lipid profile in perimenopausal overweight women

The effect of brown and golden flaxseeds on lipid profile, oxidative stress, intestinal permeability, endotoxemia, and fasting glycaemia of perimenopausal overweight women was investigated in this clinical trial. Thirty participants were divided into control (CG), brown flaxseed (BF), and golden flaxseed (GF) groups. BF and GF received 40 g of brown and golden flaxseed for 12 weeks. Venous blood samples were collected at the beginning and at the end. Intestinal permeability analysis was performed by urinary excretion of lactulose and mannitol. There was significant reduction in intestinal permeability in flaxseed groups, with delta of lactulose/mannitol ratio smaller (p ≤ 0.05). LPS levels were reduced in the flaxseed groups, whereas low-density lipoproteins (LDL) was decreased in the GF group (p ≤ 0.05). Flaxseed consumption did not change oxidative stress markers and glycaemia. Flaxseed consumption, especially golden flaxseed, reduced intestinal permeability and improved the lipid profile, showing positive effects on metabolic changes caused by menopausal transition. HIGHLIGHTS Brown and golden flaxseeds show a high content of insoluble fibre and alpha-linolenic acid, and brown flaxseed presented higher antioxidant activity. Golden flaxseed improved the lipid profile. Brown and golden flaxseeds reduced intestinal permeability and endotoxemia. Brown and golden flaxseed can be a promising alternative for the prevention of metabolic changes caused by menopausal transition, and for the improvement of the intestinal health.

Sensory and Functional Properties of Linum usitatissimum (Flaxseed) Incorporated Pulse based Ready to Cook (RTC) Health Mix for Women of Reproductive Age

Background: Scientific evidence supports therapeutic properties of flaxseed; however, a large sector of the population is still unaware of the associated benefits and its possible applications as usage in food prodcuts. Flaxseed is popularly known for its high alpha-linolenic acid content, but is also a source of lignan, fibre and protein that are biologically active in the prevention of some non-transmissible chronic diseases. Methods: Methodology involved in the study has focused on formulation of health mix by standardising the amount of flaxseed incorporated, sensory evaluation of the formulated product by semi-trained panel members, assessment of functional properties and analysis of macronutrient composition. Result: The developed health mix is a good source of protein (15 g per 100 g) and fiber (13 g per 100 g). Water soluble index of 5.23 per cent, swelling power 12.50 per cent and solubility index of 17.62 per cent was observed in the developed flour. Health mix containing 10 g of flaxseed per serving had best acceptance.

Increase in alpha-linolenic acid content by simultaneous expression of fatty acid metabolism genes in Sesame (Sesamum indicum L.).

The online version contains supplementary material available at 10.1007/s12298-022-01152-0.

Effect of geographical origin on lipid content and fatty acids composition of bee collected pollen

Among the essential nutrients found in bee pollen, lipids comprise one of its principal and widely researched compounds with variable content and fatty acids composition depending on pollen’s botanical and geographical origin. Although plant source effect on pollen lipids has been partially studied, the impact of geographical origin remains unclear and also, the analysis of polyfloral pollen samples from diverse regions may lead to inaccurate results. Thus, in the present study, monofloral bee pollen samples, Cistus criticus, and Sinapis arvensis, were collected from different areas to identify the effect of geographical origin on pollen lipid profile, excluding the impact of botanical origin. The total lipid content of Cistus pollen ranged from 2.50% to 3.24% and of Sinapis pollen from 7.64% to 9.06%, while statistically significant differences were found among the studied areas, both for the two species. However, it seems that regardless of origin, Sinapis pollen is a rich source of total lipid content, contrary to Cistus pollen. Likely, it was found that geographical origin affected their fatty acid composition. The presence of oleic acid (cis C18:1) was dominant in all Cistus pollen samples and ranged from 50.75% (Central Macedonia) to 65.78% (Attiki), while all Sinapis pollen samples exhibited high content of alpha-linolenic acid (C18:3), with the low average percentage (41.71%) found in Crete and the highest (52.94%) in Central Macedonia. The results showed that the determination of geographical origin effect on lipids profile of bee pollen should be considered for the establishment of global quality criteria.

Low temperature combined with high inoculum density improves alpha-linolenic acid production and biochemical characteristics of Chlamydomonas reinhardtii

Chlamydomonas reinhardtii grows fast and is rich in polyunsaturated fatty acids. To explore whether the alpha-linolenic acid (ALA) content can be further enhanced, the cultures were incubated under different culture temperatures, light intensities and inoculum densities. Results showed that temperature exhibited more great impact on ALA synthesis of C. reinhardtii than light intensity and inoculum size. The changes of light intensity and inoculum size displayed non-significant effects on ALA content. The optimal ALA proportion in cells was obtained under the condition of 10 °C, 50 μE/m2/s and 5% inoculum density, which reached ∼ 39%.The augmented initial inoculum density could markedly improve the biomass of C. reinhardtii under 10 °C. The maximum ALA productivity (16.42 mg/L/d) was gained under 10 °C coupled with 25% inoculum size, where higher intracellular sugar and protein yield were observed. These results suggest C. reinhardtii would be an alternative feedstock for the industrial production of ALA.

The Effect of Flaxseed Oil Supplementation on Body Composition and Inflammation Indices in Overweight Adults With Pre-Diabetes.

Background:Flaxseed has rich content of alpha linolenic acid for preventing pro inflammatory process. The aim of present study is exploring the effect of flaxseed oil supplementation on inflammatory biomarkers and body composition in overweight adults with pre-diabetes.Material and methods:this double-blind randomized clinical trial conducted with 80 overweight pre-diabetic patients in 2 clusters (flaxseed oil group (2000-mg daily) and control group) across a 14-week period. Anthropometric indices, body composition and inflammatory indices were measured between 2 groups before and after the treatment.Result:this study found a significant decrease in visceral fat level in the intervention group (P = .009) and control group (P = .004) at the end of the survey. However, the mean change of it (P = .06) was not significant. Also, this study showed that percentage of body fat (P = .31) and its mean change (P = .6) did not have significantly different between the 2 groups at the 14th week. The skeletal muscle% had a significant rise in the intervention group (P = .005) and control group (P = .003) by the end of 14th week. However, the mean change of it (P = .19) was not significant between the 2 groups. In addition, there was no significant change in the fasting blood glucose (P = . 7), C reactive protein (P = .12) and TNF-α (P = .22) between the 2 groups at the end of study.Conclusion:It showed that flaxseed oil supplementation cannot improve body composition and inflammation.

Fatty Acid Contents and Stability of Oyster Nut Oil (Telfairia pedata) Compared to Flaxseed and Sunflower Oil.

The selection of healthy fats for consumption is important. Linoleic acid (LA) (omega-6) and alpha-linolenic acid (ALA) (omega-3) are essential polyunsaturated fatty acids required for the maintenance of good health; however, LA derivatives such as arachidonic acid (AA) are associated with the onset of inflammatory diseases, and both are prone to oxidation and deterioration. This study compared the fatty acid contents, peroxide value (PV), p-anisidine value (p-AV), and free fatty acids (FFA) of the oyster nut oil with refined sunflower, nonrefined sunflower, and flaxseed oil stored at 27°C for 40 days. Flaxseed oil had significantly high ALA content (59.8%) compared to 0.1-0.5% for oyster nut and sunflower oil brands. The LA content was high in sunflower brands (50.3-52.8%) compared to the oyster nut (48%) and flaxseed oil 14.7%. Oleic acid was lower in oyster nut oil (8.6%) and flaxseed oil 15.8% compared to sunflower brands (35.7-38.2%). As a consequence, oyster nut and flaxseed recorded higher PV of 4.35-2.88 mEq O2/kg and FFA 0.26-0.47% compared to sunflower brands. The p-AV recorded small values which were not significantly different in all samples. Although oyster nut is widely consumed by pregnant and lactating women across Africa, its keeping quality in nonrefined form is low compared to flaxseed and sunflower oil as shown in this study. Hence, the fatty acid contents in oyster nuts should be consumed in other alternative forms such as flour and roasted kernels rather than its oil when in nonrefined form. This study will enable the consumption balance of omega-6/omega-3 fatty acids and the keeping quality of oils which is key to health.

Dietary intake and biomarkers of alpha linolenic acid and risk of all cause, cardiovascular, and cancer mortality: systematic review and dose-response meta-analysis of cohort studies

ObjectiveTo examine the associations between dietary intake and tissue biomarkers of alpha linolenic acid (ALA) and risk of mortality from all causes, cardiovascular disease (CVD), and cancer.DesignSystematic review and meta-analysis...

Genetic segregation analysis of unsaturated fatty acids content in the filial generations of high-linolenic-acid rapeseed (Brassica napus)

Alpha linolenic acid (ALA) is an essential polyunsaturated fatty acids that can improve human health. Rapeseed is the second largest oil crop in the world but the ALA content in its seed fatty acids is only about 10%. Two rapeseed germplasms YH25005 and R8Q10 with high content (up to 21%) ALA were developed by intervarietal crossing. They were used as the maternal parent (P1) when crossed with a low ALA parent SW (P2) to produce the seeds of the F1 hybrid, F2, and backcrosses to P1 (BCP1) and P2 (BCP2). A multigeneration joint segregation analysis was conducted to determine major gene ​+ ​polygene effects of the content of three major unsaturated fatty acids including oleic (OA), linoleic (LA) and ALA. The results showed that, although some genes favorable to ALA accumulation were not allelic in R8Q10 and YH25005, all the inheritances of OA, LA and ALA in YH25005 ​× ​SW and R8Q10 ​× ​SW followed a genetic model of ‘two pairs of additive major genes ​+ ​additive-dominant polygenes’. It is suggested that the contents of OA, LA and ALA are closely related and the major genes in the parent SW containing loss-of-function mutations in FAD2 and FAD3 loci had strong effect to reduce ALA and elevate OA. However, total genetic effect of the polygenes was greater than that of the two major genes, especial on ALA content. It indicated that it is necessary to employ a larger F2 population to find the plant that accumulate enough minor-effect polygenes for high ALA content. The results are useful for high ALA rapeseed breeding and future work of gene mapping.