Concentration In Medium Research Articles

Physiological Response of Three Melon Varieties Applied to Trichokompos Planting Media and Various Concentrations of Liquid Organic Fertilizer

The aim of this research is to determine the effect of providing trichocompost planting media composition, varieties and various concentrations of liquid organic fertilizer on the physiology of melon plants. This experiment used a separate plot design in groups, the Main Plot was the planting medium (m) which consisted of 3 planting media compositions, m1: Soil Composition: Trichokompos = 1:1, m2 : Soil Composition : Trichokompos = 1:2, m3: Soil Composition : Trichokompos = 2:1 and Subplots are 3 types of melon varieties (v), namely: v1 = Glamor F1, v2 = Rio F, v3 = Noni F1, and Children's Plots are the concentration of eco farming (k), namely: k0 = Without eco farming (control), k1 = Eco farming 5 ml/ liter of water, k3 = Eco farming 10 ml/ liter of water. The results of this research indicate that the composition of 2 soil planting media: 1 trichocompost, Rio F1 variety and a liquid organic fertilizer concentration of 1 ml/liter of water is recommended to improve the physiological phase of melon plants.

Media Ownership and Concentration in the United States of America 1984-2022

Media Ownership and Concentration in the United States of America 1984-2022

Adsorptive removal of heavy metal ions from model aqueous media using titanium metal-organic framework and its polyvinyl chloride functionalized composite

The present study was conducted on Ti-MOF and its PVC functionalized composite with the objective of toxic heavy metal ions removal from aqueous media. For achieving this purpose, the solvothermal method was used to synthesis Ti-MOF which was then functionalized with PVC for composite synthesis. The synthesized adsorbent materials were then analyzed via different techniques that include the FTIR, XRD, SEM and EDX analysis along with TGA and point of zero charge (PZC). Batch experiments were conducted for nickel (Ni) and copper (Cu) metal ions sorption from water, using the synthesized adsorbents, under different experimental conditions like initial metal ions concentration, pH, adsorbent’s dosage, time of contact and temperature. The sorption of heavy metal ions by the adsorbents was increased with increasing the media dosage and concentration. Whereas, increasing the pH of the system, from pH-2 to pH-10, also enhanced the sorption phenomenon, because of the pronounced forces of attraction between the positively charged adsorbate ions and the adsorbent’s negatively charged surface, accompanied by precipitation at a much higher pH. It was observed that the adsorption on the functionalized adsorbent surface was greater than the parent MOF material because of the availability of a greater number of adsorption sites, increasing the adsorption capacity of Ni ions from 6.436 to 29.2 mg/g and that of Cu ions from 21.414 to 41.616 mg/g. In addition, the composite material was found to have greater affinity towards Cu metal ions as compared to Ni metal ions, which is attributed to the smaller size of Cu metal ions as compared to Ni ions. To determine the adsorption mechanism, various adsorption isotherms were examined. The results concluded that the Langmuir adsorption isotherm was the suitable model to explain the adsorption mechanism.Moreover, thermodynamic parameters like Gibbs free energy (, change in enthalpy, along with the change in entropy, were determined. The sorption process was found to be more spontaneous at lower temperatures, with the exothermic nature of adsorption, in the case of Ni metal ions and the endothermic nature in the case of Cu metal ions.Also, the negative value of ΔS in case of Ni ions adsorption accounts for the decreased disorderliness of the system which is also the opposite tothat of Cu ions adsorption onto the surface of Ti-PVC composite.The PVC functionalized Ti-MOF was thus found to be an effective, durable, and environmentally friendly adsorbent, for the sorption of heavy metal ions from wastewater.

Effects of Jianpi Huayu Decoction on Th1/Th2 Immune Balance in Mice With Liver Cancer-Related Fatigue via the IL- 27/STAT1 Signaling Pathway.

Objective: The Chinese medicine Jianpi-Huayu decoction (, JPHY) can alleviate cancer-related fatigue in patients with liver cancer. However, its mechanism remains unclear. In this study, we used BALB/c mice with liver cancer model to investigate whether JPHY alleviates cancer-related fatigue by regulating Th1/Th2 immune balance; and the possible association with the IL-27/STAT1 signaling pathway. Methods: We established a mouse model of liver cancer fatigue. Mice were gavaged with physiological saline, low, medium, or high concentrations of JPHY respectively; and intraperitoneal injection of fludarabine (STAT1 pathway inhibitor) with JPHY for 21 days. We recorded the general condition of the mice, and assessed fatigue using scoring criteria and Exhausted Swimming Test. We calculated the spleen and thymus indices, performed H&E staining and immunohistochemical analysis on liver tumor tissues to observe the tumor proliferation marker ki67. We quantified the secretion levels of IFN-γ and IL-2 produced by Th1 cells in serum and splenic lymphocytes, as well as the secretion of IL-4, IL-10 by Th2 cells, and IL-27 in the signaling pathway through ELISA analysis. We evaluated the expression levels of p-STAT1 and STAT1 in spleen tissues using Western blot analysis. Results: JPHY exhibits a therapeutic effect on hepatocellular carcinoma-induced splenomegaly in murine models by upregulating the pro-inflammatory cytokines IFN-γ and IL-2 and downregulating the anti-inflammatory cytokines IL-4 and IL-10. Moreover, JPHY suppresses ki67 expression, reduces tumor-related inflammation infiltration, and ameliorates cancer-associated fatigue. Additionally, the expression of phosphorylated protein p-STAT1 is down-regulated. Conclusion: JPHY may improve the Th1/Th2 immune balance through its anti-inflammatory effects and promotion of IL-27-induced STAT1 phosphorylation, thereby alleviating fatigue in mice with liver cancer.

First principles study of Be-doped graphdiyne as anode material for lithium-ion batteries

The performances of beryllium-doped graphdiyne (GDY), which is used as an anode material for lithium-ion batteries at various doping sites, are investigated by first-principles methods based on density functional theory. Calculations of the formation energy and cohesive energy of GDY at different doping concentrations indicate that beryllium-doped GDY has excellent prospects for experimental synthesis. More importantly, the beryllium-doped GDY exhibits good electrical conductivity. The adsorption energy for a single lithium atom on beryllium-doped GDY is –4.22 eV, which is significantly higher than that for boron, nitrogen-doped GDY, and intrinsic GDY. As the number of stored lithium atoms increases, the adsorption energy remains greater than the cohesive energy of solid lithium, and the average open-circuit voltage stays between 0 and 1 V, ensuring the safety of the battery. Additionally, the lithium storage capacity is increased to 881 mAh/g, which is 1.14 times that of undoped GDY and 2.36 times that of graphite. Meanwhile, the diffusion performance of lithium on beryllium-doped GDY is also enhanced. For the C<sub>III</sub> site doping system, by studying the ion transports at low, medium, and high lithium concentrations, we find that as the lithium concentration increases, the diffusion barriers are 0.38, 0.44, and 0.77 eV, respectively, making lithium ion movement more difficult, but still superior to those of other element-doped GDY. In summary, beryllium-doped GDY has great potential as an excellent anode material for lithium-ion batteries.

Procena primene azotnog đubriva i nano-silicijum spreja na performanse i komponente prinosa semena suncokreta

Considering the climatic altering and the noteworthy increment within the late seasonal dry stress on sunflower in semi-arid regions, it is fundamental to utilize modern administration like application nano silicon to reduce the unfavourable impacts of such stresses. A field trial was performed to study the effects of nitrogen levels (40, 80, and 120 kg ha-1 ) and foliar spray treating of nano silicon (50, 100, and 150 ppm) on some traits of sunflower. The first two factors explained 79% of the variation and showed strong positive association of yield and thousand-seed weight. The N4-Si3 (120 kg ha-1 nitrogen plus 150 ppm nano silicon) indicated the higher values for most traits, leaf and head properties and seed yield. For confirmation findings of factor analysis, the clustering of traits and treatments was performed and categorized the measured traits and treatments into three clusters. The medium and high concentrations of nitrogen and nano silicon (80 and 120 kg ha-1 nitrogen; 100 and 150 ppm nano SiO2) were grouped with each other in the same cluster and were the best treatments. The nitrogen is one of the important fertilizers for sunflower production and low magnitudes of nitrogen could not be sufficient in semiarid environments. The nano silicon has beneficial ability to minimize the harmful influences of environmental stresses and can provide the goals of sustainable and low input agricultural systems in production of sunflower under semi-arid regions.

The experience of using a mineral chelate additive for growing juveniles of the African sharptooth catfish (Clarias Gariepinus) in a recirculating aquaculture system.

Growing fish in a recirculating aquaculture system (RAS) involves various stress factors that directly affect their physiological condition. This study aimed to investigate the effect of a chelated organic-mineral additive including Fe, Mn, Cu, Zn, Co, Se and I; on the rearing performance of juvenilecatfish (Clariasgariepinus) under RAS conditions. Four groups of fish (n=50) were formed: a control group (receiving standard feed) and three experimental groups (receiving standard feed with biogenic elements chelate compounds in different concentrations). At the end of the experiment (30 days), the physiological condition of the fish was evaluated by the growth rate, internal organs condition, blood serum biochemical indices and histological examination of the middle intestine. The survival rate of fish in the experimental groups was 96-98%. According to the results, the absolute increase was 14,30% in group III, 11,13% in group II and 6,71% in group I, compared to the control. However, the use of chelated compounds in high concentrations can cause necrosis and erosion of the apical part of the villi (groups II and III). Blood biochemical analysis of fish (group II and III) receiving medium and high concentrations of chelated compounds showed high ALT activity, which was 23,02% (p<0,05) and 45,19% (p<0,05) higher compared to control, respectively. Mineral-chelate compounds, of the studied composition, at a concentration of 0,5 g/kg positively affect the histological structure of the midgut of Clarias gariepinus. This dosage of the investigated feed additive can be recommended for the practical application of rearing Clariasgariepinus in closed water installations.

Analysis of rotational grazing management for sheep in mixed grassland.

Sown mixed grassland is rarely used for livestock raising and grazing; however, different forages can provide various nutrients for livestock, which may be beneficial to animal health and welfare. We established a sown mixed grassland and adopted a rotational grazing system, monitored the changes in aboveground biomass and sheep weights during the summer grazing period, measured the nutrients of forage by near-infrared spectroscopy, tested the contents of medium- and long-chain fatty acids by gas chromatography, and explored an efficient sheep fattening system that is suitable for agro-pastoral interlacing areas. The results showed that the maximum forage supply in a single grazing paddock was 4.6 kg DM/d, the highest dry matter intake (DMI) was 1.80 kg DM/ewe/d, the average daily weight gain (ADG) was 193.3 g, the DMI and ADG were significantly correlated (P<0.05), and the average feed weight gain ratio (F/G) reached 8.02. The average crude protein and metabolizable energy intake by sheep were 286 g/ewe/d and 18.5 MJ/ewe/d respectively, and the n-6/n-3 ratio of polyunsaturated fatty acids in mutton was 2.84. The results indicated that the sheep fattening system had high feed conversion efficiency, could improve the yield and quality of sheep, and could be promoted in suitable regions.

Protective effect of Phyllostachys edulis (Carrière) J. Houz against chronic ethanol-induced cognitive impairment in vivo.

Chronic alcohol consumption causes oxidative stress in the body, which may accumulate excessively and cause a decline in memory; problem-solving, learning, and exercise abilities; and permanent damage to brain structure and function. Consequently, chronic alcohol consumption can cause alcohol-related diseases. In this study, the protective effects of Phyllostachys edulis (Carrière) J. Houz (PE) against alcohol-induced neuroinflammation and cognitive impairment were evaluated using a mouse model. Alcohol (16%, 5 g/kg/day for 6 weeks) and PE (100, 250, and 500 mg/kg/day for 21 days) were administered intragastrically to mice. PE showed a protective effect against memory deficits and cognitive dysfunction caused by alcohol consumption, confirmed through behavioral tests such as the T-maze, object recognition, and Morris water maze tests. Additionally, PE attenuated oxidative stress by reducing lipid oxidation, nitric oxide, and reactive oxygen species levels in the mice's brains, livers, and kidneys. Improvement of neurotrophic factors and downregulation of apoptosis-related proteins were confirmed in the brains of mice fed low and medium concentrations of PE. Additionally, expression of antioxidant enzyme-related proteins GPx-1 and SOD-1 was enhanced in the liver of PE-treated mice, related to their inhibitory effect on oxidative stress. This suggests that PE has both neuroregenerative and antioxidant effects. Collectively, these behavioral and histological results confirmed that PE could improve alcohol-induced cognitive deficits through brain neurotrophic and apoptosis protection and modulation of oxidative stress.

Effect of calcium chloride concentration on seed germination and young seedling parameters in rice (Oryza Sativa L.)

The study was conducted with five rice varieties (Osmanchik 97, Gala, Luna, CL 34, Cameo) in order to determine and compare the effect of increased concentrations of CaCl2 on seed germination during the early stages of plant development. Variations include: control (deionized distilled water), 50 mM, 100 mM, 150 mM, 200 mM, 250 mM, and 300 mM CaCl2 concentrations. Germinating energy, germination rate, germination rate coefficient, germination index degree, germination index, mean germination time, sprout and root length, sprouts and roots fresh and dry weight, seed vitality index and salinity tolerance were determined. The data were analyzed with SPSS program. The results show that the application of increasing concentrations of CaCl2 salinity in the order of 50-200 mM leads to a prolongation of the mean germination time of the seeds and has an inhibitory effect on the growth of sprouts and roots, this effect being more pronounced on the roots . The cultivars Osmanchik 97 and Gala are relatively more tolerant to salinization with low and medium concentrations of CaCl2 in terms of seed germination. At salinity levels from 250 mM to 300 mM CaCl2 solution, all tested cultivars were sensitive.

A Comparison and Evaluation of Two Commercially Available Metal Artifact Reduction Applications.

The angiography systems A (A) and B (B), both incorporated at our hospital, are equipped with metal artifact reduction (MAR) applications. In clinical practice, it is crucial to understand the characteristics of MAR in both systems given that endovascular treatments are occasionally administered with both. In this study, we compared the artifact reduction effects of MAR on equipment A and B and clarified the differences between the two systems. An artifact evaluation phantom was created using a cylindrical water phantom and an iodine contrast medium. The phantom was imaged, MAR processing was performed on the obtained images, and an isotropic quantitative evaluation of artifacts was performed by extreme value statistical analysis using the Gumbel distribution. The MAR reduction effects were approximately 45% and 40% for equipment A and B at concentrations of 8300 and 6000, respectively. The MAR reduction effect in both devices exhibited different trends depending on the concentration. In clinical procedures that make use of absorbents in medium concentrations of approximately 3000-5000, such as n-butyl-2-cyanoacrylate and Onyx, it is necessary to understand the MAR characteristics of both devices and consider the use of alternative devices as an option.

THE GREAT RESET: “COUNTERPOWER” IN THE CONTEXT OF MEDIA CONCENTRATION AND PLATFORM DEPENDENCE

The growing concentration of power and dependence on few platforms in the media sector necessitate regulatory measures to counter the potential threats to media pluralism and editorial independence stemming from this concentration. While some legal initiatives aim to address the imbalanced power dynamics between platforms and news media, such as the efforts at the EU level through the Digital Services Act (DSA) and Digital Markets Act (DMA) to establish a fair playing field in digital markets, it is crucial to empower countervailing forces. This article explores the concept of "counterpower" within the context of media concentration and platform dependence, delving into its theoretical and practical implications. The practical analysis is grounded in 12 semi-structured interviews conducted with news organisations of various sizes in the UK and the Netherlands, revealing a heightened awareness of the necessity to reduce dependences and promote more direct and engaged journalism. The interviews identified specific strategies, albeit with some limitations, highlighting the need for additional support, especially for local news organisations striving for autonomy in reducing dependences. In a nutshell, the article examines the legal prerequisites for news organisations to establish a "counterpower," serving as a complementary piece of the larger puzzle in addressing the broader challenges of media concentration and platform dependence. Finally, alongside the evolving EU regulatory framework, encompassing the DSA, DMA, and EMFA, there is a growing demand for enabling “counterpower” and developing robust media (concentration) laws in Europe, particularly focusing on safeguarding local journalism.

Optimization and Analysis of Polyhydroxyalkanoate (PHA) by Bacillus sp. Strain CL33 and Bacillus flexus Strain S5a from Palm Oil Mill Waste

Polyhydroxyalkanoate (PHA) is a biodegradable polymer that microorganisms can synthesize amidst non-optimal growth conditions with excess carbon sources. Palm oil, rich in fatty acids, serves as a carbon source for PHA synthesis. The bacterial PHA production can be influenced by carbon concentration in the growth medium. Therefore, determining the optimal concentration of palm oil as a carbon source is crucial for PHA production. Additionally, it is possible to determine the type of PHA generated by bacteria, which can then be utilized as information when processing utilizing the PHA. The experiment employed palm oil concentrations of 0.5%, 1%, and 2% and was carried out for periods of 48, 72, 96 hours. It was discovered that Bacillus sp. strain CL33 and Bacillus flexus strain S5a produced the most effective PHA at a concentration of 25 with an incubation period of 96 hours. The PHA generated by these bacteria was quantitatively analyzed through measurements of total bacterial growth, cell dry weight, and the levels of crotonic acid. PHA types were also analyzed using GC-MS, with monomers including 2-hydroxybutyrate(-2HB), 2-hydroxy-3-phenylpropionate (2H3PhP), 3-Hydroxyhexanoate (3HHx), 3-hydroxyoctanoate (3H2O), and 3-hydroxydecanoate (3HD). The Bacillus sp. strain CL33 yielded a PHA level of 92.23%. Meanwhile, Bacillus flexus strain S5a synthesized a polyhydroxyalkanoate comprising mostly 3-hydroxyhexanoate (3HHx) and polydimethylsiloxane (PDMS). The monomers used were decamethyltetrasiloxane, dodecamethylpentasiloxane, hexamethylcyclotrisiloxane, octamethylpentasiloxane, and dodecamethylcyclohexasiloxane. The type of PHA produced accounted for 85.93% of the total.

Assessment of Grape Cuttings Rooting Behavior (Vitis Vinifera L.) under Various Media and IBA Concentrations

Assessment of Grape Cuttings Rooting Behavior (Vitis Vinifera L.) under Various Media and IBA Concentrations

Comparison of Growth of Chlorella vulgaris in Flat-Plate Photobioreactor Using Batch, Fed-Batch, and Repeated Fed-Batch Techniques with Various Concentrations of Walne Medium

Chlorella vulgaris was autotrophically grown in a Flat-Plate Photobioreactor (FPPB) using batch (BC), fed-batch (FB), and repeated fed-batch (RPF) cultivations. BC stage was done in the 20 L of working volume. During FB stages, 20 L of fresh medium was added to reach the final volume of 60 L. In the RPF stages, 20 L of broth was harvested, and then a similar volume of fresh medium was added to keep the working volume. This study aimed to evaluate the effect of Walne media (with various concentrations of composition) and cultural techniques on algal growth. Three different concentrations of Walne medium (original [coded as ´1], double [coded as ´2], and triple [coded as ´3] in concentrations of the original medium) was used for cultivations. Not only culture techniques but also various media affected biomass and chlorophyll productivity. RPF (with ´2 cultivation) gave the highest biomass productivity. However, RPF (with ´1 cultivation) gave the highest chlorophyll productivity. So, this study showed much fundamental information for the industrial production of Chlorella vulgaris biomass using flat-plate photobioreactors.

Development of porcine embryos cultured in media irradiated with ultraviolet-C.

Sterilization of the culture medium using ultraviolet (UV)-C reduces the potential adverse effects of microorganisms and allows for long-term use. In the present study, we investigated the effects of a medium directly irradiated with UV-C prior to invitro culture on the development and quality of porcine invitro-fertilized embryos and the free amino acid composition of the culture media. The culture media (porcine zygote medium [PZM-5] and porcine blastocyst medium [PBM]) were irradiated with UV-C at 228 and 260 nm for 1 and 3 days, respectively. Next, the culture media were irradiated with UV-C at 228 nm for 3, 7, or 14 days. After invitro fertilization, the embryos were cultured in the UV-C-irradiated media for 7 days. Free amino acid levels in culture media irradiated with 228 and 260 nm UV-C for 3 days were analysed. The blastocyst formation rate of embryos cultured in media irradiated with 260 nm UV-C for 3 days was significantly lower than that of embryos cultured in non-irradiated control media. However, 228 nm UV-C irradiation for up to 14 days did not affect blastocyst formation rates and quality in the resulting blastocysts. Moreover, 260 nm UV-C irradiation significantly increased the taurine concentration in both culture media and decreased methionine concentration in the PBM. In conclusion, UV-C irradiation at 228 nm before invitro culture had no detrimental effects on embryonic development. However, 260 nm UV-C irradiation decreased embryo development and altered the composition of free amino acids in the medium.

Preparation and Characterization of Boza Enriched with Nonfat Dry Milk and Its Impact on the Fermentation Process.

Boza is an indigenous, traditional, low-alcohol and highly viscous beverage prepared by fermenting cereals. Its thick and gel-like consistency make it suitable for consumption via spoon. Although boza is a nutritious beverage, its protein content is very low (<2%). A new type of boza was developed by incorporating nonfat dry milk (NFDM) to elevate the protein content of the beverage. Different NFDM amounts (10 to 40% w/v) were added to determine the best concentration and fermentation time based on the refractive index and pH values at room temperature (0-48 h). The best sample was further characterized by rheological analyses and Fourier transform infrared (FTIR) spectroscopy. The sample with 10% NFDM was the best, as fermentation was successfully performed, and further addition of NFDM increased the initial pH. The refractive index and pH decreased from 21.9 ± 0.1 to 11.8 ± 0.1 and 5.77± 0.50 to 4.09 ± 0.35 during fermentation, respectively. The samples exhibited shear-thinning, solid-like behavior, and a gel-like structure. FTIR analysis by independent modeling of class analogy (SIMCA) demonstrated that unfermented slurry and the fermented product could be effectively differentiated. With the addition of 10% NFDM, the increase in the protein content of the boza medium became significant.

Effect of growth medium composition on the viability and genetic stability of Chrysanthemum × morifolium Ramat. explants under in vitro cold storage conditions

Cultivation of plants at low positive temperatures is promising for the in vitro preservation of valuable Chrysanthemum × morifolium Ramat. varieties. The study aims to examine the effect of chlorocholine chloride concentrations in a specialized medium on the viability and the physiological and morpho-anatomical parameters of chrysanthemum plants (Excel, Sheer Purple, and William Seward varieties), as well as their genetic stability after 12 months of cold storage under in vitro gene bank conditions. In vitro microshoot tips (without leaves) were placed on a 1/4 Murashige and Skoog medium supplemented with 60.0 g/L sucrose and 0.2–0.8 g/L chlorocholine chloride. The explants were stored in cooling chambers at a temperature of 4 °C. After 12 months of preservation, a reduction was observed in growth kinetics with increasing concentration of chlorocholine chloride in the medium. The viability of explants decreased from 96 to 91% at higher concentrations of chlorocholine chloride. The effective photochemical quantum yield of photosystem II decreased more than twice under cold storage conditions as compared to the control. Several histological features were identified in the leaves of in vitro explants cold-stored in the gene bank: decreased in leaf thickness and mesophyll density in leaf blades, degradation of palisade tissue, and a high degree of transpiration regulation. The SSR and ISSR analyses were used to show that the microshoots of the examined chrysanthemum varieties were identical to the original plants following in vitro cold storage. The conducted studies revealed that the presence of 0.6–0.8 g/L chlorocholine chloride in the medium reduces the growth kinetics of explants while maintaining their high viability and genetic stability.

Effect of metallic redox ions on the corrosion behavior of GTAW weldment of type 304L stainless steel in simulated non-radioactive PUREX and HLLW medium

Nuclear-spent fuel reprocessing and high-level liquid waste (HLLW) storage containers possess various inorganic higher valance oxidizing metallic ions and fission products, further influencing nitric acid corrosion through the PUREX route. This study evaluated the effect of nitric acid corrosion on gas-tungsten arc weldment (GTAW) of stainless steel (SS) 304 L in simulated reprocessing and HLLW medium in non-radioactive conditions. The effect of oxidizing metallic cations, which were present as the major amount in the mark-I spent fuel of fast breeder test reactors (FBTR) in India; these are Cs+1, Nd+3, Mo+6, Fe+3, and V+5 as a surrogate redox system for Pu(VI)/Pu(IV) and Np(VI)/Np(V) system was evaluated by the electrochemical corrosion test method in 2 M, 6 M, and 8 M nitric acid solutions. The resistance of polarization (Rp) value decreases with the nitric acid concentration, and the effect of oxidizing ions in different nitric acid concentrations also changes. The three-phase corrosion (boiling liquid, vapor, and condensate phase) resistance behavior in different concentrations (6 M and 8 M) of the nitric acid medium, without and with redox ions, has been evaluated. The corrosion rate is higher in the liquid phase compared to the vapor and condensate phases. On the other hand, it is relatively higher for the inorganic metallic redox ions containing solutions.

Secretagogue effect of PDE4 inhibitor apremilast on human salivary gland organoids obtained from primary Sjögren's syndrome patients.

The aim of the study was to culture vital salivary gland organoids obtained through labial or parotid biopsy of primary Sjögren's syndrome (pSS) patients in order to evaluate their morphological and functional features in basal condition and after stimulation with Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) activator forskolin and phosphodiesterase 4 (PDE4) inhibitor apremilast, their in vitro regenerative capacity and the immune-histological resemblance with original tissue. Salivary gland tissues from five pSS patients were processed to obtain vital organoids; swelling assay and cell proliferation tests were performed after forskolin and apremilast application. Immunochemistry evaluation on original salivary gland tissue and corresponding organoids was performed, and secretomics analysis was conducted to assess their functional status. After application of forskolin and apremilast, we observed organoid swelling after 30 minutes, compatible with a positive functional status and enhancement of saliva production. In 3 cases, apremilast induced organoid proliferation. All cases were positive for cytokeratin 14 (CK14) and most for cytokeratin 5 (CK5). All the cases were positive for amylase; its secretion, and thus functional status of organoids, was confirmed by its high concentration in the culture medium. A focal ductal differentiation was found in some cases, highlighted by epithelial membrane antigen (EMA) positivity. The more differentiated EMA positive areas were negative for the staminal marker CK14, showing a sort of "complementary staining". Our data highlighted that differentiated cells and vital functional organoids that recapitulate the development of original salivary glands can be obtained from pSS epithelium. For the first time, the direct stimulating effect of PDE4 inhibitor apremilast on pSS human salivary gland organoids is reported, opening new perspectives on targeting oral dryness with drugs that combine secretagogue and immunomodulatory effects.