Fish Tissue Concentrations Research Articles

Distribution of trace elements in the aquatic ecosystem of the Thigithe river and the fish Labeo victorianus in Tanzania and possible risks for human consumption

The aim of the present study was to assess the distribution of trace elements in the aquatic ecosystem of the Thigithe river. Samples of surface water, sediment and fish were collected up- and downstream of the North Mara Gold Mine (Tanzania) and following trace elements were analysed: As, Cd, Co, Cr, Cu, Hg, Ni, Pb and Zn. Trace element concentrations in surface water were below or near the detection limit. Regarding the sediments, relative high concentrations of arsenic at all sites and high levels of mercury at a site downstream of the mine where artisanal mining is performed were observed. Trace element concentrations in Ningu fish tissues (Labeo victorianus) were comparable to slightly higher than levels in fishes from unpolluted environments. For none of the measured human health risk by consumption of fish from the Thigithe river is expected when the Tanzanian average amount of 17g/day is consumed. However, for Hg and As the advised maximum daily consumption of Ningu fish was lower than 100g. As a result fishermen and people living along the shores of the river consuming more fish than the average Tanzanian fish consumption set by the FAO (2005) are possibly at risk.

Developing Ambient Water Quality Criteria for Mercury: A Probabilistic Site-Specific Approach

This project examined the development of ambient water quality criteria (AWQC) for the protection of wildlife for mercury. Mercury is considered a serious risk to wildlife in many areas. As a result, the Great Lakes Water Quality Initiative and others have developed AWQC. These AWQC have been controversial, however, because (1) the AWQC were single values that did not account for site-specific conditions; (2) derivation of the AWQC relied on a single NOAEL, and (3) the AWQC had an unknown level of conservatism because of reliance on both average and conservative assumptions and uncertainty factors. Rather than develop a single value AWQC for total mercury, we derive an AWQC model that explicitly incorporates factors controlling bioavailability, methylation rates and bioaccumulation in the aquatic environment (e.g., pH, DOC, sulfate). To derive our AWQC model, field data was collected including numerous water quality parameters and total mercury and methylmercury concentrations in whole body fish tissue from 31 lakes in Ontario and an additional 10 lakes in Nova Scotia. An independent dataset consisting of 51 water bodies in the United States was then used to confirm the validity and robustness of the AWQC model. Next we combined the results of chronic-feeding studies with similar protocols and endpoints, in a meta-analysis to derive a dose-response curve for mink exposed to mercury in the diet. Using this approach, one can derive an LD5 or other similar endpoint that can then be used as the basis for deriving -wildlife AWQC. In the final step, we used a probabilistic risk model to estimate the concentrations of methylmercury in water that would lead to levels in fish sufficient for there to be a 10% probability of exceeding the mink LD5. This analysis was repeated for various combinations of pH and DOC. The result is an AWQC model for mercury for the protection of wildlife that can be used for a variety of site-specific conditions. This title belongs to WERF Research Report Series ISBN: 9781843396376 (Print) ISBN: 9781780404028 (eBook)

Mercury, selenium and fish oils in marine food webs and implications for human health.

Humans who eat fish are exposed to mixtures of healthful nutrients and harmful contaminants that are influenced by environmental and ecological factors. Marine fisheries are composed of a multitude of species with varying life histories, and harvested in oceans, coastal waters and estuaries where environmental and ecological conditions determine fish exposure to both nutrients and contaminants. Many of these nutrients and contaminants are thought to influence similar health outcomes (i.e., neurological, cardiovascular, immunological systems). Therefore, our understanding of the risks and benefits of consuming seafood require balanced assessments of contaminants and nutrients found in fish and shellfish. In this paper, we review some of the reported benefits of fish consumption with a focus on the potential hazards of mercury exposure, and compare the environmental variability of fish oils, selenium and mercury in fish. A major scientific gap identified is that fish tissue concentrations are rarely measured for both contaminants and nutrients across a range of species and geographic regions. Interpreting the implications of seafood for human health will require a better understanding of these multiple exposures, particularly as environmental conditions in the oceans change.

Persistent Organochlorine Pesticide Exposure Related to a Formerly Used Defense Site on St. Lawrence Island, Alaska: Data from Sentinel Fish and Human Sera

St. Lawrence Island, Alaska, is the largest island in the Bering Sea, located 60 km from Siberia. The island is home to approximately 1600 St. Lawrence Island Yupik residents who live a subsistence way of life. Two formerly used defense sites (FUDS) exist on the island, one of which, Northeast Cape, has been the subject of a $123 million cleanup effort. Environmental monitoring demonstrates localized soil and watershed contamination with polychlorinated biphenyls (PCB), organochlorine (OC) pesticides, mercury, and arsenic. This study examined whether the Northeast Cape FUDS is a source of exposure to OC pesticides. In total, 71 serum samples were collected during site remediation from volunteers who represented three geographic regions of the island. In addition, ninespine stickleback (Pungitius pungitius) and Alaska blackfish (Dallia pectoralis) were collected from Northeast Cape after remediation to assess continuing presence of OC pesticides. Chlordane compounds, DDT compounds, mirex, and hexachlorobenzene (HCB) were the most prevalent and present at the highest concentrations in both fish tissues and human serum samples. After controlling for age and gender, activities near the Northeast Cape FUDS were associated with an increase in serum HCB as compared to residents of the farthest village from the site. Positive but nonsignificant relationships for sum-chlordane and sum-DDT were also found. Organochlorine concentrations in fish samples did not show clear geographic trends, but appear elevated compared to other sites in Alaska. Taken together, data suggest that contamination of the local environment at the Northeast Cape FUDS may increase exposure to select persistent OC pesticides.

Total iron concentrations in waters and fish tissues in the Nam Theun 2 Reservoir area (Lao PDR).

Data on total iron concentrations in waters and freshwater fish tissues in man-made reservoirs are scarce, especially in Southeast Asia. Changes in total iron concentrations in water and in fish tissues were studied after the impoundment of the Nam Theun 2 Reservoir (central Lao PDR). Water quality parameters were monitored at 11 stations (reservoir, upstream area and downstream areas) from 2008 to 2010. In 2009 and 2010, total iron concentrations were measured in three different fish tissues (viscera, gills and flesh) from 14 species belonging to the omnivorous and carnivorous feeding groups. The results indicated that iron concentrations in the water were influenced by the reservoir impoundment during the first year after the creation of the reservoir. Intra-annual variations of the total iron concentration in these waters appeared to be driven by the soil leaching processes mainly during the wet season. In fish, total iron accumulated preferentially in viscera, followed by gills and flesh. Iron concentration was highly species dependant and related to the ecology of the species whereas feeding habits (omnivorous or carnivorous) did not influence total iron concentration in fish tissues. Finally, reservoir impoundment did not affect iron concentrations in fish from the reservoir and from both downstream areas.

Assessment of trace metals in Amazonian fish exposed to untreated urban sewage: high chromium concentrations in fish tissues

The aim of this study was to evaluate the quality of fish meat in relation to inorganic contaminants (Zn, Pb, Cu, Cr, Ni and Hg), following the Brazilian Health Surveillance Agency (ANVISA) standards in five Amazonian fish exposed to untreated urban sewage. Trace metal concentrations were evaluated in the muscle tissues of Schizodon fasciatus (herbivorous), Potamorhina latior (detritivorous), Triportheus angulatus (omnivorous), Plagioscion squamosissimus (carnivorous) and Rhaphiodon vulpinus (piscivorous), sampled upstream and downstream of Santo Antonio’s Hydroelectric Dam, Madeira River (Porto Velho City, Brazil). An acid chemical digestion was used to extract trace metals from the tissues, and atomic absorption spectrometry methods were used to quantify the trace metal concentrations. Fish samples showed values above allowed limits for Cr (0.10 mg.kg -1 ), except in Plagioscion squamosissimus (0.09 mg.kg -1 ). Potamorhina latior specimens sampled downstream showed higher values for Cr (p<0.0001) than specimens sampled upstream. This result may indicate that untreated urban sewage discharged into the river downstream is influencing the Cr concentrations. As only Cr exceeded the allowed value for consumption, the estimated daily intake of Cr in the Brazilian urban and riverine population was calculated based on their fish consumption for each species sampled upstream and downstream. Estimates of daily intake of Cr were higher in fish sampled downstream than upstream, and were 13 times higher in the riverine population than in the Brazilian urban population.

Tissue concentrations as the dose metric to assess potential toxic effects of metals in field-collected fish: Copper and cadmium.

The present study examined the available literature linking whole-body tissue concentrations with toxic effects in fish species for copper and cadmium. The variability in effect concentration for both copper and cadmium among species occurred within an order of magnitude for all responses, whereas the range for lethal toxicity based on water exposure spanned approximately 4 to 5 orders of magnitude. Fish tissue concentrations causing adverse effects were just above background concentrations, occurring between 1 μg/g and 10 μg/g for copper and 0.1 μg/g to 4 μg/g for cadmium. The results also show that salmonids are especially sensitive to cadmium, which appears to be a function of chemical potency. No studies were found that indicated adverse effects without increases in whole-body concentration of these metals. This narrow range for dose-response implies that a toxicological spillover point occurs when the detoxification capacity of various tissues within the animal are exceeded, and this likely occurs at a similar whole-body concentration for all naïvely exposed fish species. Elevated whole-body concentrations in fish from the field may be indicative of possible acclimation to metals that may or may not result in effects for target species. Acclimation concentrations may be useful in that they signal excessive metal concentrations in water, sediment, or prey species for a given site and indicate likely toxic effects for species unable to acclimate to excess metal exposure. Using tissue residues as the dose metric for these metals provides another line of evidence for assessing impaired ecosystems and greater confidence that hazard concentrations are protective for all fish species.

Active biomonitoring of a subtropical river using glutathione-S-transferase (GST) and heat shock proteins (HSP 70) in Oreochromis niloticus as surrogate biomarkers of metal contamination

The aim of this study was to establish the level of water quality impairment along a mine effluent receiving river, Pote River in Zimbabwe, using Oreochromis niloticus (Nile tilapia) as an indicator organism. Glutathione-S-transferase (GST) enzyme and heat shock protein (HSP 70) expression in the stomach tissue of Nile tilapia were used as biomarkers of heavy metal pollution. Water and fish samples were collected at three sites in the Pote River. Metal concentration in tilapia tissues and water were measured using flame acetylene absorption spectrophotometry while enzymatic activity was determined using ultra-violet spectrophotometry. The concentration of Ni and Zn in water was significantly high compared to the World Health Organization aquatic effluent standards. Cd and Zn were strongly associated with high GST activity and low HSP 70 protein concentrations in fish tissues. Results of this study show that HSP 70 protein and GST enzyme activity can be useful biomarkers of metal contamination in lotic systems. Keywords : enzymes, biomarkers, heavy metals, Nile tilapia, bioassessment, Pote River

Spatial and temporal variation of total mercury and methylmercury in lacustrine wetland in Korea.

The spatial and temporal variations of total mercury (THg) and methylmercury (MeHg) concentrations (n = 120, respectively) in water and sediments of the Yangsuri lacustrine wetland in Korea were measured. The average concentrations of THg and MeHg in surface water were 2.04 ± 1.97 and 0.05 ± 0.05 ng L(-1), respectively. The concentrations of THg and MeHg in sediments ranged from 1.28 to 85.83 and ≤0.01 to 0.35 ng g(-1), respectively, and varied depending on the location. In the vegetated zone located near residential areas, the highest concentrations of THg and MeHg in both surface water and sediments were obtained near the residential areas, especially during the fall season. This result might be due to increased methylation rate of Hg by water turnover in the fall season. While THg and MeHg concentrations in water were not correlated, they were significantly correlated in sediment (r = 0.75; P < 0.01). Log-transformed Hg concentrations in sediments were highly correlated with log-transformed organic matter (OM) and acid volatile sulfide (AVS) concentrations (P < 0.01). We also collected five species of fish near the output point of the wetland, and MeHg concentrations in fish tissue varied from 21.30 to 154.66 μg kg(-1) w/w, which was significantly dependent on fish species (P < 0.05). This is the first reported study which measured the levels of mercury, especially MeHg in the wetlands, and freshwater fish species in Korea.

Tritium uptake in rainbow trout (Oncorhynchus mykiss): HTO and OBT-spiked feed exposures simultaneously

There is currently considerable interest in organically bound tritium (OBT) formation in edible fish. The major questions revolve around whether or not tritium can accumulate in fish after being released into aquatic environments. Since OBT formation rates in large, edible fish are poorly understood, rainbow trout (Oncorhynchus mykiss) studies, where fish were simultaneously exposed to tritiated water (HTO) and OBT-spiked feed over 130 days, were conducted to evaluate tritium uptake.The measured HTO activity concentrations in fish tissue confirmed that HTO in fish tissue equilibrates quickly with HTO in tank water. The data obtained also confirmed that OBT uptake is faster when fish are ingesting OBT-spiked feed compared to when fish are living in tritiated water (and consuming non-OBT-spiked feed). The difference between the two exposure types is such that the groups exposed to tritiated water and OBT-spiked feed simultaneously were showing the same uptake rates as OBT-spiked feed only exposures. Contrary to what was expected, the rate of OBT uptake (from OBT-spiked feed) seemed to be higher in slow growing fish compared to fast growing fish. Another observation from these studies was that OBT activity concentrations in all organs (viscera) had a tendency to be higher than OBT activity concentrations measured in fish flesh.

Assessment of Heavy Metals in Some Marine Fish Species Relevant to their Concentration in Water and Sediment from Coastal Waters of Ondo State, Nigeria

Due to oil exploration activities and urbanization as well as continuous industrial and agricultural growth in Ondo State, Nigeria. The coastal water has been heavily impacted by a number of pollutants originating from different sources including heavy metals. This investigation assessed and monitored accumulation levels of ( Cr, Cd, Pb, Cu, Zn, and Ni ) in different organs ( Gill, Head, Bone, Muscle and Eye ) of five fish species (Pentanemus quinquarius, Pseudoltolithus senegalensis, Trichirus lepturus, Plectorhynchus meditarraneus and Pseudotolithus typus together with water and sediment collected from the ocean shoreline in the coastal waters of Ondo State, Nigeria. Heavy metals concentrations in fish tissues, water and sediment samples were analyzed after treatment using atomic absorption spectrophotometer. Dissolve total metal in water (in mg/l) Zn 0.12-0.22 , Cu 0.26-0.27 and Ni BDL-0.01 recorded in the water samples in this study were low and within the maximum permissible level (MPL) recommended by WHO and USEPA, while higher concentrations above the MPL were recorded for Cr 0.31-0.34 Cd 0.08 -0.21 and Pb 0.57-0.79. The concentrations of these metals in sediment in (mg/kg) were in the range Cr (0.16-4.19, Cd 0.35-0.38, Pb 0.9-1.00, Cu 0.24-1.44, Zn 2.12-3.11 and Ni BDL-0.01. They are all lower than their probable effect concentrations (PEC) in sediment. Mean Concentrations of the heavy metals in fish species were of the order: Zn>Cu>Cr>Cd>Pb>Ni. Levels of heavy metals varied depending on different tissues in the fish species. Correspondingly, high concentrations of the metals were found in gills and eye compared to other tissues. While the concentration of Zn (0.34 mg/kg-1.29 mg/kg), Cu (BDL-1.88 mg/kg), Ni (BDL-0.01 mg/kg) , and Cr (BDL-1.28) in the fish tissues were within the maximum allowable level (MAL) for a food source, the findings of this study shows higher value above the allowable limit for Cd(BDL-1.14 mg/kg) and Pb (BDL-0.71 mg/kg), thus constituting potential health hazard to consumers of these fish species.

Assessing ecological risks to the fish community from residual coal fly ash in Watts Bar Reservoir, Tennessee.

Extensive site-specific biological and environmental data were collected to support an evaluation of risks to the fish community in Watts Bar Reservoir from residual ash from the December 2008 Tennessee Valley Authority (TVA) Kingston ash release. This article describes the approach used and results of the risk assessment for the fish community, which consists of multiple measurement endpoints (measures of exposure and effects) for fish. The lines of evidence included 1) comparing postspill annual fish community assessments with nearby prespill data and data from other TVA reservoirs, 2) evaluating possible effects of exposures of fish eggs and larval fish to ash in controlled laboratory toxicity tests, 3) evaluating reproductive competence of field-exposed fish, 4) assessing individual fish health through physical examination, histopathology, and blood chemistry, 5) comparing fish tissue concentrations with literature-based critical body residues, and 6) comparing concentrations of ash-related contaminants in surface waters with US Environmental Protection Agency's (USEPA) Ambient Water Quality Standards for Fish and Aquatic Life. These measurement endpoints were treated as independent lines of evidence that were integrated into an overall weight-of-evidence estimate of risk to the fish community. Collectively, the data and analysis presented here indicate that ash and ash-related constituents pose negligible risks to the fish communities in Watts Bar Reservoir. This conclusion contradicts the predictions by some researchers immediately following the ash release of devastating effects on the aquatic ecology of Watts Bar Reservoir. The information presented in this article reaffirms the wisdom of carefully evaluating the evidence before predicting probable ecological effects of a major event such as the TVA Kingston ash release. This study demonstrates that a thorough and detailed investigation using multiple measurement endpoints is needed to properly evaluate ecological effects.

Euthynnus affinis (little tuna): fishery, bionomics, seasonal elemental variations, health risk assessment and conservational management

The fishery, biology, growth and stock structure of Euthynnus affinis is studied in detail. Hooks and lines, gillnets and purse seines are the major equipment used to exploit the fish. Fisheries are sustained mainly by 1–2 year old fishes (34–50 cm). Spawning was observed around the year with peaks during July–August and November–January. The length–weight relationship is 0.0254 L2.889 with no significant difference between males and females. Age and growth are estimated using length based methods. The maximum sustainable yield estimated was higher than the average annual catch, indicating scope for further exploitation. Elevated levels of heavy metals in Euthynnus affinis may be a good indication of pollution of an aquatic ecosystem due to anthropogenic influences. A total of 278 fishes were collected from Karachi coast, Fish Harbor West Wharf, Karachi, for metal (Cd, Pb, Zn and Cu) analysis in the organs of the fish. The metal levels in the sample fishes are in descending order of toxicity Cd>Pb>Zn>Cu. In the risk assessment, we assessed potential human health risks associated with consumption of fish, incorporating information gathered during a year-long, intercept-style creel angler survey and representative heavy metal concentrations in fish tissue. Fishing operations can cause ecological impacts of different types, e.g. by the catches, damage of the habitat, mortalities caused by lost or discarded gear, pollution, and generation of marine debris. Periodic reassessment of the tuna potential is required, with adequate inputs from exploratory surveys as well as commercial landings; this may prevent any unsustainable trends in the development of the tuna fishing industry in the Arabian Sea.

Effectiveness of a sediment time critical removal action-PCB reduction in fish tissue, surface water, and sediment via wet excavation.

Documenting successful remediation of polychlorinated biphenyl (PCB)-contaminated sediments is limited; potentially due to inadequate monitoring methods, complexities associated with the environment, and selected remedial techniques. At some sites, absence of appropriate baseline and postremoval monitoring limits proper evaluation of remedial efficacy. Accurate interpretation of interactions between media, space, time, species, lipid content, and remedial technique requires robust study design and data. This article presents baseline and postremoval data documenting reduced PCB concentrations in fish tissue, surface water, and sediment in response to the US Environmental Protection Agency (USEPA) time-critical removal action (TCRA) that was conducted at the former Bryant Mill Pond (BMP) on Portage Creek in Kalamazoo, Michigan. The BMP is part of an operable unit (OU) within the Allied Paper, Inc./Portage Creek/Kalamazoo River Superfund Site. PCBs discharged to the creek as a byproduct of carbonless copy paper recycling are the primary contaminant of concern. Paper waste residuals commonly appear as gray to light gray clays in river sediments and floodplain soils. The cleanup criterion was 10 mg/kg, with a residual PCB concentration goal of 1 mg/kg. Because the PCB-containing waste is (generally) associated with readily visible light gray clay, excavation of all visibly contaminated current or formerly impounded sediment served as a surrogate for the cleanup criteria and goal. Sediment was wet excavated and backfilled after diversion of the creek. After confirmation that PCB concentrations met cleanup criteria, the stream was diverted to the excavated side, and excavation and backfilling were completed. Overall, 146000 cubic yards of material including PCB-contaminated sediments were removed from the BMP. The long-term monitoring (LTM) program implemented by the Michigan Department of Environmental Quality (MDEQ), and historic data from a variety of sources (federal, state, and local agencies and responsible parties) provided data demonstrating TCRA effectiveness. Before TCRA, surface sediment PCB concentration in instream and formerly inundated sediment combined was 83 mg/kg (with a maximum of 700 mg/kg). Instream sediments that are more representative of fish exposures had a pre-TCRA surface weighted average concentration (SWAC) of 27 mg/kg. The post-TCRA SWAC for instream and floodplain sediments combined was 0.26 mg/kg. Average surface water concentrations at the downstream end of the BMP were reduced from 0.11 µg/L pre-TCRA to 0.0025 µg/L post-TCRA. Tissue samples for adult carp fillets decreased from 4 mg/kg pre-TCRA to 0.26 mg/kg post-TCRA; whole body white suckers from 3 mg/kg pre-TCRA to 0.1 mg/kg post-TCRA; whole body channel catfish from 39 mg/kg-L pre-TCRA to 2.6 mg/kg-L post-TCRA. Concentrations of PCBs in 2 species of resident fish (carp and white suckers), caged channel catfish, surface water, and sediment were reduced by over 1 order of magnitude within 1 year of completion, substantively accelerating natural recovery processes. A slight increase in PCB concentration was observed in both whole body suckers and adult carp fillets in the second monitoring period post-TCRA; however, these concentrations are still near an order of magnitude less than pre-TCRA concentrations and appear to be currently stable or on a slight downward trend.

Threshold-dependent sample sizes for selenium assessment with stream fish tissue.

Natural resource managers are developing assessments of selenium (Se) contamination in freshwater ecosystems based on fish tissue concentrations. We evaluated the effects of sample size (i.e., number of fish per site) on the probability of correctly detecting mean whole-body Se values above a range of potential management thresholds. We modeled Se concentrations as gamma distributions with shape and scale parameters fitting an empirical mean-to-variance relationship in data from southwestern West Virginia, USA (63 collections, 382 individuals). We used parametric bootstrapping techniques to calculate statistical power as the probability of detecting true mean concentrations up to 3 mg Se/kg above management thresholds ranging from 4 to 8 mg Se/kg. Sample sizes required to achieve 80% power varied as a function of management thresholds and Type I error tolerance (α). Higher thresholds required more samples than lower thresholds because populations were more heterogeneous at higher mean Se levels. For instance, to assess a management threshold of 4 mg Se/kg, a sample of eight fish could detect an increase of approximately 1 mg Se/kg with 80% power (given α=0.05), but this sample size would be unable to detect such an increase from a management threshold of 8 mg Se/kg with more than a coin-flip probability. Increasing α decreased sample size requirements to detect above-threshold mean Se concentrations with 80% power. For instance, at an α-level of 0.05, an 8-fish sample could detect an increase of approximately 2 units above a threshold of 8 mg Se/kg with 80% power, but when α was relaxed to 0.2, this sample size was more sensitive to increasing mean Se concentrations, allowing detection of an increase of approximately 1.2 units with equivalent power. Combining individuals into 2- and 4-fish composite samples for laboratory analysis did not decrease power because the reduced number of laboratory samples was compensated for by increased precision of composites for estimating mean conditions. However, low sample sizes (<5 fish) did not achieve 80% power to detect near-threshold values (i.e., <1 mg Se/kg) under any scenario we evaluated. This analysis can assist the sampling design and interpretation of Se assessments from fish tissue by accounting for natural variation in stream fish populations.

Microcystin-LR equivalent concentrations in fish tissue during a postbloom Microcystis exposure in Loskop Dam, South Africa

The effects of a decomposing cyanobacteria bloom on water quality and the accumulation of microcystin-LR equivalent toxin in fish at Loskop Dam were studied in May 2012. Enzyme-linked immunosorbent assay [ELISA] was used to confirm the presence of microcystin-LR equivalent in the water and to determine the microcystin (MCYST) concentration in the liver and muscle of fish. The lowest concentration of extracellular MCYST-LR equivalent was recorded in the lacustrine zone, where no cyanobacterial cells were observed, while the highest concentration (3.25 µg l−1), 3.25 higher than World Health Organization standard, was observed in the riverine zone. Extremely high MCYST-LR equivalent concentrations of 1.72 µg MCYST-LReq kg−1 in the liver and 0.19 µg kg−1 in muscles of Labeo rosae, and 2.14 µg MCYST-LReq kg−1 in the liver and 0.17 µg kg−1 in muscles of Oreochromis mossambicus, indicate that the consumption of sufficient fish biomass might cause severe adverse effects in humans. Microscopic analyses of the stomach content of both fish species revealed low numbers of cyanobacterial Microcystis aeruginosa cells in comparison to other phytoplankton. The extracellular MCYST-LR equivalent of the decomposing bloom may have played a major role in the high levels observed in the livers of the two fish species. These findings are important for all downstream water users.

Mercury (Hg) and methyl mercury (MMHg) bioaccumulation in three fish species (sea food) from Persian Gulf

In this study, mercury (Hg) and methyl mercury (MMHg) were determined in three fish species including benthic, benthopelagic and pelagic fish from Arvand river, northwest of Persian Gulf. Mercury and methyl mercury levels varied significantly depending on the tissues, sex and species. The contaminated concentrations were highest in liver whereas lowest in the muscle of all fish species. In this study, there was a direct relationship between contaminated levels in tissues organisms with habit and feeding habitats. The results of this study show that highest mean mercury level were found in the benthic fish (E. diacanthus), followed by benthopelagic fish (P. niger), and pelagic species (A. indicus). The mean levels of mercury in E. diacanthus was 0.05 μg g−1, P. niger 0.05 μg g−1 and in A. indicus was 0.05 μg g−1. The mean levels of methyl mercury E. diacanthus was 0.05 μg g−1, P. niger 0.05 μg g−1 and in A. indicus was 0.05 μg g−1. Result of regression analysis showed that there were significant relationships between mercury and methyl mercury concentrations in fish tissues.

Assessment of Fluoride in Three Selected Polluted Environments along the Egyptian Mediterranean Sea: Effects on Local Populations

ABSTRACTFluoride ion (F−) is a relevant component of concern due to its potential toxicity for humankind. This study clarifies the status of F− for human health in three polluted regions along the Egyptian Mediterranean Sea coast, and determining its contents in seawater and different fish tissues (muscle, skin, brain, and bone). Fluoride ion concentrations in different fish tissues had a descending trend of brain > skin > muscle > bone with averages of 26.8 ± 26.0, 24.8 ± 6.5, 12.7 ± 2.3, and 9.0 ± 1.6 μg F−/g wet wt, respectively. The bioconcentration factor for fluoride ion in different fish tissues was determined and multivariate analyses were conducted. The human health hazard assessment from fish consumption and seawater contact using hazard quotient (HQ) and ingestion and dermal absorption dose of whole body for different ages of females and males as well as the specified body parts (legs, arms, and hands) were calculated. Although the calculated daily intake values for ingestion of fish in the present study were lower than some established tolerable daily intake values, their hazard quotients were within 1 ≥ HQ < 10 and may reflect a possible fluoride adverse effect on human health from the consumption of the gathered fish species.

Modeling Mercury Distribution in the Sarasota Bay Ecosystem Using SERAFM and Stable Isotope Ratios of Nitrogen (δ15N) in Biota

Abstract Conversion of CH3Hg+ concentrations in water to CH3Hg+ concentrations in fish tissue is critically important in characterizing Hg health risks to humans and biota in aquatic ecosystems. However, this is a challenging task due to complex biogeochemical CH3Hg+ processes that are replete with uncertainty. In the present study, the uncertainty was reduced by employing the Spreadsheet-based Ecological Risk Assessment for the Fate of Mercury (SERAFM) model and a linear relationship between logarithmic Hg concentrations and stable isotope ratios of nitrogen (δ15N) in biota. By using this approach, the fate, transport, and bioaccumulation of CH3Hg+ in Sarasota Bay, FL, was modeled. Modeling results suggest that ∼60% of the total Hg loading (8.7 kg/year) to the Sarasota Bay in 2011 originated from atmospheric Hg deposition to the watershed with subsequent transport to the waterbody via stormwater runoff. This indicated that watershed runoff could be an important source of Hg to a bay adjacent to highly ur...

Pre-anthropocene mercury residues in North American freshwater fish

Mercury (Hg) has been entering the environment from both natural and anthropogenic sources for millennia, and humans have been influencing its environmental transport and fate from well before the Industrial Revolution. Exposure to Hg (as neurotoxic monomethylmercury [MeHg]) occurs primarily through consumption of finfish, shellfish, and marine mammals, and regulatory limits for MeHg concentrations in fish tissue have steadily decreased as information on its health impacts has become available. These facts prompted us to consider 2 questions: 1) What might the MeHg levels in fish tissue have been in the pre-Anthropocene, before significant human impacts on the environment? and 2) How would these pre-Anthropocene levels have compared with current regulatory criteria for MeHg residues in fish tissue? We addressed the first question by estimating pre-Anthropocene concentrations of MeHg in the tissues of prey and predatory fish with an integrated Hg speciation, transport, fate, and food web model (SERAFM), using estimated Hg concentrations in soil, sediment, and atmospheric deposition before the onset of significant human activity (i.e., ≤2000 BCE). Model results show MeHg residues in fish varying depending on the characteristics of the modeled water body, which suggests that Hg in fish tissue is best considered at the scale of individual watersheds or water bodies. We addressed the second question by comparing these model estimates with current regulatory criteria and found that MeHg residues in predatory (but not prey) fish could have approached or exceeded these criteria in some water bodies during the pre-Anthropocene. This suggests that the possibility of naturally occurring levels of Hg in fish below which it is not possible to descend, regardless of where those levels stand with respect to current regulatory limits. Risk management decisions made under these circumstances have the potential to be ineffectual, frustrating, and costly for decision makers and stakeholders alike, suggesting the need for regulatory flexibility when addressing the issue of Hg in fish.