Total Soluble Sugar Content Research Articles

Integrative analysis of metabolome and genome-wide transcriptome reveal the flavor changes in apple (Malus pumila Mill) after the novel acaricide cyflumetofen application

Pesticide residues were found to interfere the nutrition and flavor of fruits. Apple flavor changes after pesticides application was investigated based on metabolic dynamics and underlying regulatory networks. In this study, cyflumetofen (CYF) systematically affected the nutrition and flavor formation on apple (Malus pumila Mill). CYF alters nutritional composition, but not total content of soluble sugars and organic acids. Palatability-related amino acid content decreased around 15% in CYF-treated apple. The contents of esters and alcohols responsible for fragrance and flavor decreased by approximately 10% in CYF-treated apples compared with controls. Non-target metabolomic and transcriptomic analysis showed that CYF mainly affected amino acid-, organic acid-, polyphenol-, and lipid-metabolism related pathways, leading to altered nutritional and flavor characteristics. In conclusion, the results suggested that CYF affected the primary metabolism of apple, resulting in unpleasant changes in nutritional and flavor composition. This study provided new insight into the metabolic regulation of flavor after pesticides application.

Effect of INM on biochemical and postharvest parameters of guava (Psidium guajava) cv. Hisar Surkha

An experiment was conducted during rainy (kharif) and winter (rabi) season of 2018–19 and 2019–20 at the research farm of CCS Haryana Agricultural University, Hisar, Haryana to study the effects of organic amendments and biofertilizers on biochemical and post-harvest parameters of guava fruits (Psidium guajava L. cv. Hisar Surkha). The experiment consisted of 19 treatments in different combinations and levels of RDF with vermicompost, farmyard manure, and biofertlizers were applied. The results revealed that almost all treatments with combined application of organic and inorganic sources of nutrients significantly influenced the biochemical and post-harvest parameters of guava fruits over control of which treatments T16 (RDF 100% + Farmyard manure + Azotobacter + PSB) and T17 (RDF 100 % + Vermicompost + Azotobacter + Phosphate solubilizing bacteria) proved most effective. All treatments had a significant impact on the total soluble solids, acidity, vitamin C, pectin and sugar content of fruit over the course of two years of experimentation. Shelf-life of fruits also increased with integrated use of 100% RDF with vermicompost and biofertilizers. Physiological loss in weight was minimum (9.73 and 18.34%; 9.61 and 18.46% after 4 and 8 days of harvesting) and shelf-life increased (4.74 and 6.85 days) in harvested fruit in T17 during rainy and winter season, respectively. The overall quality of the fruit was higher throughout the winter than during the rainy season. Hence, a portion of chemical fertilizers can be reduced in fertilization of guava plants through the integrated application of different organic amendments with improvement in different quality parameters.

Regulation of Vicia faba L. Response and Its Effect on Megoura crassicauda Reproduction under Zinc Stress.

The heavy metal zinc (Zn) is known to be transmitted in the food chain; however, the effect of Zn stress on beans and herbivorous insects is largely unclear. This study aimed to investigate the resistance of broad bean plants to Zn stress and the consequent changes in their physiological and biochemical metabolism by simulating heavy metal pollution in soil. Simultaneously, the effects of aphid progeny treated with different Zn concentrations on the expression of carbohydrate and related genes were analyzed. The results showed that Zn had no effect on the germination rate of broad beans, but other effects mainly manifested as follows. (1) Chlorophyll content decreased. (2) The total soluble sugar and Zn content in stems and leaves increased with increasing Zn content. (3) The proline content first increased and then decreased with increasing Zn content. (4) The height of the seedlings indicates that low concentrations promote growth and high concentrations inhibit growth. In addition, only the first-generation fecundity decreased significantly when aphids fed on heavy metal broad beans. Continuous high Zn levels increase the trehalose content of aphid F1 and F2, while F3 decreases. These results can not only provide a theoretical basis for exploring the impact of soil heavy metal pollution on ecosystems but also preliminarily evaluate the possibility of broad beans as a means of pollution remediation.

Physiological and Nutrient Responses to Nitrogen, Phosphorus, or Potassium Deficiency of Hydroponically Grown Strawberry

Nutrient-depleted soil is a major constraint for crop production, particularly for fruits. Here, we investigated the different response of nitrogen (N), phosphorus (P), and potassium (K) deficiency on the growth and development of strawberry (Fragaria ×ananassa Duch.) in sand culture under greenhouse conditions. Compared with K, the lack of N or P is more unfavorable to strawberry growth and development. N deficiency affected shoot-root (S/R) ratio at different growth stages, and decreased the shoot biomass. P deficiency greatly increased the N content but decreased K content of the plants, which means P is of advantage to regulate the absorption and utilization of N and K nutrients in plants. Meanwhile, P has a profound influence on fruit quality, such as total soluble (TSS) sugar content. K deficiency is not conducive to fruit coloring and the formation of high-quality commercial fruits. The results contribute to a better understanding of the difference of N, P, or K deficiency on strawberry growth, nutrient absorption, and fruit quality during the whole growth period.

Effects of organic fertilizers on yield, soil physico-chemical property, soil microbial community diversity and structure of Brassica rapa var. Chinensis.

The amount of chemical fertilizer for vegetables is on the high level in China. The use of organic fertilizers to meet the nutrient requirement of crops will be an inevitable practice in sustainable agriculture. In this study, we compared the effects of pig manure fertilizer, rabbit manure fertilizer and chemical fertilizer on yield, quality of Brassica rapa var. Chinensis, soil physico-chemical properties and microbial community by using two consecutive seasons of three fertilizers in a pot experiment. The results were as follows: (1) In the first season, the fresh yield of Brassica rapa var. Chinensis applying chemical fertilizer was significantly (p ≤ 5%) higher than those of applying the pig manure and rabbit manure fertilizer, and the results were the opposite in the second season. The total soluble sugar concentration of fresh Brassica rapa var. Chinensis applying rabbit manure fertilizer was significantly (p ≤ 5%) higher than those of applying pig manure fertilizer and chemical fertilizer in the first season, and the NO3-N content of fresh Brassica rapa var. Chinensis on the contrary. (2) The organic fertilizer increased the concentration of total nitrogen, total phosphorus and organic carbon in soil in both two seasons. Rabbit manure fertilizer increased the soil pH and EC and significantly (p ≤ 5%) reduced the soil NO3-N content. (3) The pig manure and rabbit manure fertilizer significantly (p ≤ 5%) increased the diversity and abundance of soil bacterial of Brassica rapa var. Chinensis, but had no significant effect on soil fungi. Pearson correlation analysis showed that soil TN, TP, organic carbon content and EC were significantly correlated with soil bacterial α - diversity. There were significant differences (p ≤ 5%) in the bacterial community structures between three treatments in two seasons, and significant differences (p ≤ 5%) in the fungal community structures between fertilizer treatments while not between two seasons. Pig manure and rabbit manure fertilizer decreased the relative abundance of soil Acidobacteria and Crenarchaeota, rabbit manure fertilizer significantly increased the abundance of Actinobacteria in the second season. Distance-based redundancy analysis (dbRDA) showed that soil EC, TN, and organic carbon content were key physico-chemical factors in determining bacterial community structure in Brassica rapa var. Chinensis soil, and soil NO3-N, EC, SOC concentration and soil pH in the fungal community structure.

Characterization of two peanut genotypes for biochemical quality attributes and cotyledon microstructure features

Peanut is a world-renowned oilseed and food crop. Improving its biochemical quality is of great significance not only for meeting the demand for edible oil and food, but also for producing clean renewable energy. Identification of peanut genotypes with high and stable oil content in coastal areas is of economic importance, as the high oil trait of peanut bred in inland areas is difficult to fully express in coastal areas. Two peanut genotypes were grown at two sites in the coastal area. One of the genotypes was identified as having high oil and low carbohydrate, while the other had low oil and high carbohydrate. We noticed that number and total area of oil bodies were higher in high oil peanut than in low oil peanut, and that peanut with higher protein content had larger total protein body area. A novel finding of the present study was that number and area of starch grains were higher in peanut with high total soluble sugar (TSS) and sucrose contents than in peanut with low TSS and sucrose. Regarding the ultrastructural properties associated with oil, protein and TSS/sucrose contents, significant and highly positive correlations were detected between total oil body area and number of oil bodies, between total protein area and maximum protein body area, and between total starch grain area and number of starch grains. This study laid the foundation for further exploration of the mechanisms shaping peanut seed biochemical quality.

Integrating LC-MS and HS-GC-MS for the metabolite characterization of the Chinese medicinal plant Platostoma palustre under different processing methods.

Platostoma palustre (or Mesona chinensis Benth) is an important medicinal and edible plant in China and Southeast Asian countries. To study the effects of different processing methods on the quality, nutrition, and flavor of P. palustre, we adopted the LC-MS and HS-GC-MS to compare the influences of tedding (S), sweating (M), and drying (H) on the metabolites and volatile substances of P. palustre. Biochemical determinations revealed that the M treatment could promote the accumulation of the contents of total sugar, soluble sugar, and total pectin compared with the H and S treatments but decrease the total flavonoid contents. LC-MS and HS-GC-MS uncovered 98 differential metabolites and 27 differential volatile substances among the three treatments, respectively. Overall, the M treatment facilitated the stabilization and improvement of the quality of polysaccharides and volatile substances, while the H treatment could promote the level of amino acids in P. palustre. The current study provided a theoretical reference for establishing standardized processing methods and sustaining the quality stability of P. palustre in future.

Quality evaluation of citrus varieties based on phytochemical profiles and nutritional properties.

China is one of the major producers and exporters of various kinds of citrus fruits. As one of China's major citrus planting bases, Sichuan has a citrus planting area that exceeds 400,000 hectares. Meanwhile, citrus cultivation has become one of the important agricultural pillar industries in the region. Citrus fruits are reported to show various health-promoting effects, especially antioxidant activity. However, reports on the functional, nutritional and qualitative characteristics of different citrus varieties in Sichuan are still scarce. The quality attributes (color parameters, shape, and size), juice properties (titratable acids and total soluble sugar), mineral elements, and health-promoting nutritional and functional components (protein, carbohydrates, fat, dietary fiber, ascorbic acid, phenolic acids, and flavonoids), as well as antioxidant properties of 10 typical citrus varieties cultivated in Sichuan, were systematically investigated and analyzed. Significant differences among different citrus varieties were found. In particular, the total soluble sugar content of Mingrijian was higher than that of other citrus, suggesting its potential for fresh consumption and food processing. Moreover, a total of five flavonoids and nine phenolic acids were identified and quantified. Yuanhong, with higher contents of ascorbic acid and phenolic acids, was considered to be a valuable variety with excellent antioxidant capacity and can be used for value-added processing in the food industry. Principal component analysis and hierarchical cluster heatmap analysis suggested that there were significant differences among the 10 citrus varieties. Correlation analysis confirmed the significant contribution of ascorbic acid and phenolic acids to antioxidant capacity in citrus. The results can provide some references for the cultivation and selection of nutritious citrus fruits.

Halotolerant bacterial biofilms for desalination and water treatment: a pilot study.

Salinity has a significant impact on the water quality and crop yield. Physical desalination techniques were once thought to be expensive and time-consuming. Among biological techniques, halotolerant bacteria were thought to be the fastest and most effective way to reduce the salt content in brackish saltwater water. In the current study, halotolerant bacterial biofilms were used to desalinate saline water on abiotic substrates (such as sand, pebbles, glass beads, and plastic beads), and studied subsequently for the effects on Zea mays germination. Briefly, salt samples (SLT7 and SLT8) from the Khewra site in Punjab, Pakistan, as well as seawater and sea sand samples (USW1, USW3, USW6, DSW1, DSW4, SS1, and SS3) from Karachi, Sindh, Pakistan's Arabian Sea, were collected. Halotolerant bacteria were isolated and characterized. Crystal violet ring assays and capsule staining were used to estimate extracellular polymeric substance (EPS) and biofilm development, respectively. All halotolerant bacterial strains were spore formers and produced EPS and formed biofilms well. 16S rRNA gene sequencing of the best halotolerant bacteria, USW6, showed the closest (100%) similarity to Bacillus aerius strain G-07 (a novel species) (accession number ON202984). A pilot-scale experiment for desalinating the artificial water (supplemented with 1M NaCl) using biofilm adhered abiotic beads showed declined level of NaCl from 1M to 0.00003M after 15days in treated water. Also, Zea mays germination was observed in the plants using treated water compared to no growth in the non-treated saline water. Estimations of chlorophyll, total soluble sugar, and protein revealed that plants cultivated using elute collected from a desalinated pilot scale setup contained less chlorophyll (i.e., 5.994 and 116.76). Likewise, plants grown with elute had a total soluble protein and sugar content of 1.45mg/ml and 1.3mg/ml, respectively. Overall, in treated water plants, a minor drop in chlorophyll content, a slight increase in total soluble sugar content, and a slight increase in protein content were noted. The study concluded that biofilm-treated desalt water has the potential to significantly reduce the effects of droughts, soil salinization, and economic and environmental issues associated with agricultural drainage. The results specified the application of halotolerant bacteria biofilms (Bacillus aerius, a novel species, USW6) for water desalination to overcome the problem of water scarcity caused by global warming and the increased salinity.

Comparative study of expression patterns of aquaporin (AQP) genes in apple fruits with contrasting ripening behavior

This research compared expression patterns of aquaporin (AQP) genes in two apple cultivars that exhibit contrasting ripening behavior. Commercially-mature apple fruits of cultivars ‘Golden Delicious’ and ‘Red Fuji’ were chosen as materials. The results showed that compared with ‘Red Fuji’, ‘Golden Delicious’ generally had higher rate of respiration, ethylene production, weight loss, dry matter concentration (DMC), titratable acidity (TA), total soluble solids (TSS) and soluble sugar contents during ripening. At 35 d, cells in ‘Golden Delicious’ were loosely arranged with larger intercellular space, while in ‘Red Fuji’, cells were still densely packed. A total of 20 AQPs were detected in both cultivars. Expression of these identified genes was in a tissue- and cultivar-specific manner during ripening. Generally, expression of MdAQPs was differentially induced at climacteric stage in peel and flesh tissues of ‘Golden Delicious’. In contrast, expression of most MdAQPs was differentially up-regulated at preclimacteric stage in peel and flesh tissues of ‘Red Fuji’. These results will be useful for further study the relationships between MdAQPs and storability of different apple cultivars.

The morpho-physiological responses of a tolerant and sensitive wheat (Triticum aestivum L.) cultivar to drought stress and exogenous methyl jasmonate

One of the most significant abiotic factors that has a negative impact on wheat productivity globally is drought. To comprehend the impacts of drought on wheat and propose remedies, numerous studies are carried out on various wheat varieties. In this study, 50 µM of methyl jasmonate (MeJA) was applied to tolerant Gün 91 and sensitive Bezostaja wheat cultivars and they were exposed to drought stress for 15 days. The responses of MeJA application on wheat development and physiology, as indicators of drought tolerance, were investigated comparatively. Wheat's morphology was negatively impacted by drought stress, which also decreased the crop’s relative water content (RWC) and protein content while raising its soluble sugar level. Furthermore, Gün 91, a tolerant cultivar, came to the fore as the cultivar with higher shoot-root length, RWC, total soluble sugar and protein contents compared to Bezostaja cultivar as a result of drought application. Exogenous MeJA application, cause to increase in content of osmolytes (total soluble sugar, protein) compared to the drought group and had an improving effect in maintaining the water status of wheat seedlings. Hence, the RWC increased from 48.90% to 66.87% in the tolerant Gün 91 cultivar, but no change was observed in Bezostaja cultivar. Applying 50 μM of MeJA increased the protein by 4.42%, total soluble sugar by 19.92%, and RWC by 36.74% in Gün 91 cultivar while increasing protein by 3.11% and total soluble sugar by 11.02% in Bezostaja cultivar. Moreover, there is not any significant effect of MeJA observed on the shoot-root length of both cultivars and the RWC of Bezostaja cultivar. When all results are evaluated together, exogenous MeJA application may positively affect the response of wheat seedlings, and minimize the damaging effects so we can suggest using MeJA and cultivars that are resistant to drought stress for wheat yield.

Effects of water stress on starch synthesis and accumulation of two rice cultivars at different growth stages.

Rice is a water intensive crop and soil water conditions affect rice yield and quality. However, there is limited research on the starch synthesis and accumulation of rice under different soil water conditions at different growth stages. Thus, a pot experiment was conducted to explore the effects of IR72 (indica) and Nanjing (NJ) 9108 (japonica) rice cultivars under flood-irrigated treatment (CK, 0 kPa), light water stress treatment (L, -20 ± 5 kPa), moderate water stress treatment (M, -40 ± 5 kPa) and severe water stress treatment (S, -60 ± 5 kPa) on the starch synthesis and accumulation and rice yield at booting stage (T1), flowering stage (T2) and filling stage (T3), respectively. Under LT treatment, the total soluble sugar and sucrose contents of both cultivars decreased while the amylose and total starch contents increased. Starch synthesis-related enzyme activities and their peak activities at mid-late growth stage increased as well. However, applying MT and ST treatments produced the opposite effects. The 1000-grain weight of both cultivars increased under LT treatment while the seed setting rate increased only under LT3 treatment. Compared with CK, water stress at booting stage decreased grain yield. The principal component analysis (PCA) showed that LT3 got the highest comprehensive score while ST1 got lowest for both cultivars. Furthermore, the comprehensive score of both cultivars under the same water stress treatment followed the trend of T3 > T2 > T1, and NJ 9108 had a better drought-resistant ability than IR72. Compared with CK, the grain yield under LT3 increased by 11.59% for IR72 and 16.01% for NJ 9108, respectively. Overall, these results suggested that light water stress at filling stage could be an effective method to enhance starch synthesis-related enzyme activities, promote starch synthesis and accumulation and increase grain yield.

Differences in ecophysiological responses of Populus euphratica females and males exposed to salinity and alkali stress

Soil salinity is usually accompanied by alkalization in northwest China, and they both negatively impact plant growth and result in severe ecological problems. Some studies have reported tree responses to salinity or alkali stress alone, however, the interactive salinity and alkali effects are still unclear, especially in dioecious trees. In this study, we measured growth, morphology, leaf stomata, gas exchange, carbon isotope composition (δ13C), total soluble sugar and starch contents, Na+ accumulation and allocation, oxidative stress, and antioxidants of female and male Populus euphratica seedlings in response to salinity, alkali and their interaction. Our study showed no significant sexual differences in studied traits under control conditions. In addition, P. euphratica females showed greater inhibitory and negative effects, such as bigger decreases in growth and gas exchange, lower stomatal density and water use efficiency (as described by δ13C), and lower levels of soluble sugars and antioxidant enzyme activities compared with males under salinity, alkali and interactive stress conditions. Furthermore, P. euphratica males had a greater ability of ion exclusion and Na + transport restriction. For example, males allocated more Na+ to stems and roots than females, whereas females had higher Na+ contents in leaves under stress conditions. In conclusion, our results indicated that P. euphratica males have superior resistance and they perform better than females under salinity, alkali and their interactive stress conditions.

Exogenous Calcium Reinforces Photosynthetic Pigment Content and Osmolyte, Enzymatic, and Non-Enzymatic Antioxidants Abundance and Alleviates Salt Stress in Bread Wheat.

One of the main environmental stresses that hinder crop development as well as yield is salt stress, while the use of signal molecules such as calcium (Ca) has a substantial impact on reducing the detrimental effects of salt on different crop types. Therefore, a factorial pot experiment in a completely randomized design was conducted to examine the beneficial role of Ca (0, 2.5, and 5 mM) in promoting the physiological, biochemical, and growth traits of the wheat plant under three salt conditions viz. 0, 30, and 60 mM NaCl. Foliar application of Ca increased the growth of salt-stressed wheat plants through increasing photosynthetic pigments, IAA, proline, and total soluble sugars contents and improving antioxidant enzymes in addition to non-enzymatic antioxidants glutathione, phenol and flavonoids, β-carotene, and lycopene contents, thus causing decreases in the over-accumulation of free radicals (ROS). The application of Ca increased the activity of antioxidant enzymes in wheat plants such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), which scavenge reactive oxygen species (ROS) and relieved salt stress. An additional salt tolerance mechanism by Ca increases the non-antioxidant activity of plants by accumulating osmolytes such as free amino acids, proline, and total soluble sugar, which maintain the osmotic adjustment of plants under salinity stress. Exogenous Ca application is a successful method for increasing wheat plants' ability to withstand salt stress, and it has a considerable impact on the growth of wheat under salt stress.

L-Glutamate maintains the quality of apple fruit by mediating carotenoid, sorbitol and sucrose metabolisms.

l-Glutamate is involved in many important chemical reactions in horticultural products and improves postharvest disease resistance. Quality decline of apple fruit caused by senescence and fungus invasion often leads to tremendous losses during logistics. This study was performed to evaluate the variations of quality attributes, carotenoid, sorbitol and sucrose metabolisms in apples (cv. Qiujin) after l-glutamate dipping treatment. l-Glutamate immersion maintained high values of L*, a* and b*, flesh firmness, titratable acidity, as well as the total soluble solids, soluble sugar, reducing sugar and ascorbic acid contents in apples. l-Glutamate also decreased mass loss, respiratory rate and ethylene release, enhanced sucrose synthase-cleavage, acid invertase and neutral invertase activities, whereas reduced sorbitol dehydrogenase, sucrose phosphate synthase, sucrose synthase synthesis and sorbitol oxidase activities in apples. Moreover, l-glutamate inhibited lutein, β-carotene and lycopene accumulation, and down-regulated phytoene synthase, lycopene β-cyclase, ζ-carotene desaturase, phytoene desaturase, carotenoid isomerase, ζ-carotene isomerase and carotenoids cleavage dioxygenase gene expressions, but up-regulated 9-cis-epoxycarotenoid dioxygenase gene expression in apples. Postharvest l-glutamate dipping treatment can keep apple quality by modulating key enzyme activity and gene expression in sorbitol, sucrose and carotenoid metabolisms. © 2023 Society of Chemical Industry.

Characterization of the Polar Profile of Bacon and Fuerte Avocado Fruits by Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry: Distribution of Non-structural Carbohydrates, Quinic Acid, and Chlorogenic Acid between Seed, Mesocarp, and Exocarp at Different Ripening Stages.

Avocado fruit growth and development, unlike that of other fruits, is characterized by the accumulation of oil and C7 sugars (in most fruits, the carbohydrates that prevail are C6). There are five essential carbohydrates which constitute 98% of the total content of soluble sugars in this fruit; these are fructose, glucose, sucrose, d-mannoheptulose, and perseitol, which together with quinic acid and chlorogenic acid have been the analytes under study in this work. After applying an efficient extraction procedure, a novel methodology based on hydrophilic interaction liquid chromatography coupled to mass spectrometry was applied to determine the levels of these seven substances in tissues─exocarp, seed, and mesocarp─from avocado fruits of two different varieties scarcely studied, Bacon and Fuerte, at three different ripening stages. Quantitative characterization of the selected tissues was performed, and the inter-tissue distribution of metabolites was described. For both varieties, d-mannoheptulose was the major component in the mesocarp and exocarp, whereas perseitol was predominant in the seed, followed by sucrose and d-mannoheptulose. Sucrose was found to be more abundant in seed tissues, with much lower concentrations in avocado mesocarp and exocarp. Quinic acid showed a predominance in the exocarp, and chlorogenic acid was exclusively determined in exocarp samples.

Integrative Analysis of Fruit Quality and Anthocyanin Accumulation of Plum cv. 'Cuihongli' (Prunus salicina Lindl.) and Its Bud Mutation.

Fruit color is one of the quality indicators to judge the freshness of a plum. The coloring process of plum skin is valuable for research due to the high nutritional quality of anthocyanins found in plums. 'Cuihongli' (CHL) and its precocious mutant variety 'Cuihongli Red' (CHR) were used to analyze the changes of fruit quality and anthocyanin biosynthesis during plum development. The results showed that, during the development of the two plums, the total soluble solid and soluble sugar contents were highest at the mature stage, as the titratable acid trended gradually downward as the fruits of the two cultivars matured, and the CHR fruit showed higher sugar content and lower acid content. In addition, the skin of CHR turned red in color earlier than CHL. Compared with CHL, the skin of CHR had higher anthocyanin concentrations, higher activities of phenylalanine ammonia-lyase (PAL), chalcone isomerase (CHI), dihydroflavonol-4-reductase (DFR), and UDPglucose: flavonoid-3-O-glucosyltransferase (UFGT), and higher transcript levels of genes associated with anthocyanin production. In the flesh of the two cultivars, no anthocyanin content was detected. Taken together, these results suggest that the mutation exerted a major effect on anthocyanin accumulation via modification of the level of transcription; thus, CHR advances the ripening period of 'Cuihongli' plum and improves the fruit quality.

Osmoregulatory and Antioxidants Modulation by Salicylic Acid and Methionine in Cowpea Plants under the Water Restriction.

Global climate changes have intensified water stress in arid and semi-arid regions, reducing plant growth and yield. In this scenario, the present study aimed to evaluate the mitigating action of salicylic acid and methionine in cowpea cultivars under water restriction conditions. An experiment was conducted in a completely randomized design with treatments set up in a 2 × 5 factorial arrangement corresponding to two cowpea cultivars (BRS Novaera and BRS Pajeú) and five treatments of water replenishment, salicylic acid, and methionine. After eight days, water stress decreased the Ψw, leaf area, and fresh mass and increased the total soluble sugars and catalase activity in the two cultivars. After sixteen days, water stress increased the activity of the superoxide dismutase and ascorbate peroxidase enzymes and decreased the total soluble sugars content and catalase activity of BRS Pajeú plants. This stress response was intensified in the BRS Pajeú plants sprayed with salicylic acid and the BRS Novaera plants with salicylic acid or methionine. BRS Pajeú is more tolerant to water stress than BRS Novaera; therefore, the regulations induced by the isolated application of salicylic acid and methionine were more intense in BRS Novaera, stimulating the tolerance mechanism of this cultivar to water stress.

Effects of roasting on the chemical compositions, color, aroma, microstructure, and the kinetics of changes in coffee pulp.

Roasting is a critical process that affects the quality attributes of coffee beans; however, how roasting conditions affect the physical, chemical, biological, and organoleptic changes of coffee pulp needs more research. In the present study, we investigated the effects of roasting temperatures and times on chemical compositions and quality attributes of coffee pulp. The results showed that the contents of total soluble sugar (TSS) and free amino acid (FAA) followed a temporal pattern of first increasing and then decreasing under the roasting temperatures between 100 and 160°C. Total phenolic content (TPC) and antioxidant activity of coffee pulp significantly (p<0.05) increased after roasting, reaching the maximum values of 83.09 mg gallic acid equivalent(GAE)/g and 360.45 µM 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid(Trolox)/g, respectively, when coffee pulp was roasted at 160°C for 18min. Drying rates of coffee pulp fitted the Logarithmic kinetic model, while color (L*, a*, and b*) changes and 5-caffeoylquinic acid degradation followed the first-order kinetic model. Electronic nose analysis showed that the main aroma compounds of the coffee pulp are sulfur-containing organics that were reduced with the extended roasting time. Scanning electronic microscopy analysis presented the loosened, shrunk, and cracked microstructure on the surface of the roasted coffee pulp, which might contribute to the increased TSS, FAA, TPC, and antioxidant activity of coffee pulp roasted under specific conditions. In conclusion, our research provides valuable information for preparing high-quality coffee pulp tea. PRACTICAL APPLICATION: This article investigates the effects of roasting on the chemical composition, color, flavor, microstructure, and the kinetics of changes in the moisture, color, and 5-caffeoylquinic acid (5-CQA) of the coffee pulp. We have found that high-temperature and short-time roasting helps retain the total phenolic contents, antioxidant activity, and aroma. The drying kinetic fits the Logarithmic model, and the changes in color and 5-CQA fit the first-order kinetic model. This study provides meaningful information for preparing coffee pulp tea with high-quality attributes and antioxidant activity.

Sterile line Dexiang074A enhances drought tolerance in hybrid rice.

Heterosis has been widely used in rice breeding, especially in improving rice yield. But it has rarely been studied in rice abiotic stress, including the drought tolerance, which is becoming one of the most important threaten in decreasing rice yield. Therefore, it is essential to studying the mechanism underlying heterosis in improving drought tolerance of rice breeding. In this study, Dexiang074B (074B) and Dexiang074A (074A) served as maintainer lines and sterile lines. Mianhui146 (R146), Chenghui727 (R727), LuhuiH103 (RH103), Dehui8258 (R8258), Huazhen (HZ), Dehui938 (R938), Dehui4923 (R4923), and R1391 served as restorer lines. The progeny were Dexiangyou (D146), Deyou4727 (D4727), Dexiang 4103 (D4103), Deyou8258 (D8258), Deyou Huazhen (DH), Deyou 4938 (D4938), Deyou 4923 (D4923), and Deyou 1391 (D1391). The restorer line and hybrid offspring were subjected to drought stress at the flowering stage. The results showed that Fv/Fm values were abnormal and oxidoreductase activity and MDA content were increased. However, the performance of hybrid progeny was significantly better than their respective restorer lines. Although the yield of hybrid progeny and restorer lines decreased simultaneously, the yield in hybrid offspring is significantly lower than the respective restorer line. Total soluble sugar content was consistent with the yield result, so we found that 074A can enhance drought tolerance in hybrid rice.