Concentration Of Toothpaste Research Articles

Fluoride Concentration, Antibacterial Effect, and Cytotoxicity in Children's Toothpaste: In Vitro Study

Abstract Objectives The aim of this study is to evaluate the effect on cell viability, antibacterial activity against cariogenic bacteria, and total fluoride availability of commercially available children's toothpastes in Thailand. Materials and Methods Seven toothpaste slurries were prepared from seven commercial toothpastes for children. Total fluoride concentration was determined. The agar diffusion method was used to examine the antibacterial effect of toothpaste against Streptococcus mutans. The viability of L929 mouse fibroblasts exposed with different concentrations of each toothpaste slurry was tested by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. Statistical Analysis One way analysis of variance (ANOVA) and Tukey's honestly significant difference (HSD) tests were used for zone inhibition analysis. Cell viability data were analyzed using Student's t-test. Results Fluoride concentration in fluoride-containing toothpastes ranged from 521.36 to 1,377.83 ppm. Two toothpastes exhibited a similar level of fluoride concentration compared between the product labels. Other toothpastes showed a difference or slight difference in fluoride concentration between the companies' information and our data. The zone of inhibition ranged from 0 to 2.08 cm. A significantly higher zone of inhibition was observed in toothpastes with sodium lauryl sulfate. The concentration of toothpaste that reduced cell viability to less than 50% of the control was 0.8, 3.1, 6.3, 6.3, 25, and 100% for Fluocaril Deli Fruity, Oral-B Junior 6 + , Kodomo Kids, CUdent Stevia, SunStar GUM, and Kindee Organic, respectively. Conclusion The examined children's toothpastes can inhibit the growth of S. mutans, which did not correlate with fluoride concentration. The antibacterial effects could be the results of other ingredients, for example, sodium lauryl sulfate.

Potensi Pasta Gigi Minyak Atsiri Temulawak (Curcuma xanthorriza Roxb) dalam Menghambat Pembentukan Plak dan Gingivitis pada Tikus yang Diinduksi Phorpyromonas gingivalis

Gingivitis is an inflammatory disease in gingiva without any loss of attachment caused by plaque. Gingivitis is reversible, if not treated it can develop into periodontitis. Gingivitis-causing bacteria include Phorpyromonas gingivalis, acting as secondary bacteria in plaque formation at the secondary colonization stage by attaching themselves to monolayers that have been formed by primary bacteria. Herbal use in Indonesia is still a trend until now with the slogan back to nature, one of the easy to find and useful is essential oil from curcuma rhizomes containing xanthorrizol and curcumin. They are antibacterial that can inhibit the formation of plaques that cause gingivitis. The purpose was to analyze the potential of curcuma essential oil toothpaste in inhibiting the formation of plaque and gingivitis. 27 wistar mice were divided into 9 groups, namely negative control group, positive control, treatment applied curcuma essential oil toothpaste concentration of 0.5%, 1%, 2%, 4%, 8%, 16% and 32%. Each mouse induced P. gingivalis 0.02 ml on molar sulkus one and two, 24 hours later performed tooth brushing with toothpaste according to the group, the next 24 hours were observed in day 3, 5 and 7 using plaque index according to Park and Katz and gingiva index according to Lee. Treatment and observation are carried out 3 repetitions. The results of the plaque index score and gingiva index in the treatment group were fewer than the negative controls. Curcuma essential oil toothpaste can inhibit the formation of plaque and gingivitis by decreasing the plaque index and gingiva index in P. gingivalis- induced wistar mice.

Formulation & Evaluation of Myrrh Toothpaste

Objectives: To formulate and measure the cytotoxicity level of organic myrrh toothpaste. Materials and Methods: Myrrh extract was prepared by the freeze drying process. Toothpaste was formulated by mixing specified amounts of myrrh extract, hydroxypropyl methylcellulose (HPMC) polymer, sodium lauryl sulphate (SLS), mint, and sucralose with deionized distilled water. MTT test was performed using concentrations of 100, 200, 300 and 400 mg/ml to assess the effect of myrrh paste on the gingival fibroblasts viability at intervals of 24 and 48hrs. The results were analysed by using SPSS version 27. Results: The formulated myrrh toothpaste has a homogeneous consistency as the extracted myrrh successfully dissolved completely with the other components. Cell viability test showed that myrrh paste concentrations of 100 - 300mg/ml were effective in maintaining the rate of fibroblasts growth after 24 and 48 hours as compared to the control samples. Cell growth rate was suppressed in the test samples treated with paste concentration of 400mg/ml. The results of the study imply that 300mg/ml is the safe and optimal concentration for fibroblasts growth, whereas concentrations ≥ 400mg/ml are intolerable and might be suppressing the proliferation of fibroblasts. Conclusion: Myrrh toothpaste concentration at 300mg/ml is the safe and optimal concentration for fibroblasts growth as depicted in the results, suggesting that at the optimal formulation, myrrh extract may not be toxic to the soft tissues and myrrh toothpaste may be useful for oral health care. Further clinical investigations are recommended to obtain the clinical efficacy data of the organic myrrh toothpaste.

Oral health status and practices of 6- to 7-year-old children in Amman, Jordan: a cross-sectional study

BackgroundDental caries affects the majority of children in Jordan, with some evidence of its prevalence steadily increasing. Previous studies have shown that families struggle to establish good oral health practices. The aim of this study was to evaluate the current oral health status and practices of 6- to7-year-old children in Amman, Jordan.rMethodsA cross-sectional cohort study. The sample consisted of 6- to 7-year-old children attending six randomly selected schools in Amman, Jordan. Measures collected were: I) Caries experience (d3mft/D3MFT), II) Oral hygiene, measured using the Simplified Oral Hygiene Index, III) Dietary, toothbrushing, and dental attendance practices, measured using diaries and parental questionnaires, IV) Participants’ basic characteristics: age, education and employment. Data were analysed using SPSS20.ResultsIn total, 942 children were recruited. Four hundred and fifty-seven were boys, 485 were girls. Their average age was 6.5 years. Eighty-nine percent had decay in their primary teeth. Mean d3mft was 5.1(1 (range = 0–12, SD = 2.9). Only 8% of carious teeth were restored. Mean DMFT score was 0.3 (range = 0–4, SD = 0.8). Mean debris score was 1.07 (range = 0–3, SD = 0.37). Children indicated that they brush their teeth 1.6 times a day (range = 0–3, SD = 0.6). The majority (81%) were unsupervised. Sixty-seven percent of parents did not know the appropriate fluoride toothpaste concentration. Children were having 1.5 sugary snacks in-between their meals (Range = 1–6, SD = 1.1). They scored a mean of 2.5 (Range = 0–5.87, SD = 1.7) in sweetened drinks intake (recommended ≤ 1) and 2.8 (Range = 0–18.57, SD = 1.5) in non-core food intake (recommended ≤ 2) on a dietary questionnaire. Most parents (84%) indicated that their child attends the dentist only when in pain, and 18% indicated that their child is extremely afraid of dentists. Only 32% and 18% were familiar with fluoride varnish and fissure sealants, respectively. Regression analysis revealed that debris score and dental attendance were reliable predictors of caries experience.ConclusionsSix- to seven-year-old children in Amman, Jordan have a high caries experience. Most show signs of poor oral hygiene, excessive intake of cariogenic foods, and symptomatic dental attendance. Their parents lack knowledge on fluoride varnish and fissure sealants. There is a need for oral health promotion tailored to this cohort's need.

Fluoride toothpastes of different concentrations for preventing dental caries.

Fluoride toothpastes of different concentrations for preventing dental caries.

Evidence‐based prevention: a comparison of oral hygiene advice given by dental and dental care professional students

This study aimed to examine the oral hygiene advice given by student dentists and dental care professionals (DCPs), focussing on adherence to evidence-based recommendations provided by the Department of Health and the Scottish Intercollegiate Guidelines Network. A self-administered questionnaire was distributed to 121 fourth- and fifth-year dental undergraduates and 38 hygiene/combined dental hygiene and dental therapy students at a UK dental school. Completed questionnaires were returned by 39/64 fourth-year and 36/57 fifth-year dental students and 23/38 student DCPs, an overall response rate of 61.6%. Only 48% (36) of dental undergraduates in comparison with 95.7% (22) of DCP students stated that they would give oral hygiene advice to every adult patient (P<0.001). In addition, only 24 (32%) responding dental students were able to accurately state the recommended fluoride toothpaste concentration for adult use; this contrasts with 18 (78.3%) student DCPs. Dental undergraduates tended to accord less importance to oral hygiene advice when compared with student DCPs, only providing it when they felt it was clinically necessary. The role of the dental practitioner in providing oral health education requires greater emphasis in the undergraduate curriculum. Given the unfavourable comparison between the attitude and knowledge of dental students and that of DCPs, prequalification training for the dental team should be integrated wherever possible. The apparent lack of awareness of current guidelines is of concern.

The effect of toothpaste concentration on enamel and dentine wear in vitro

Objectives To determine the effect of toothpaste concentration on enamel and dentine wear in vitro and to model the effects of toothpaste abrasion upon dilution during toothbrushing in vivo. Methods Polished human enamel/dentine specimens were indented with a Knoop diamond and the enamel–dentine junction profiled. Specimens were brushed for 1200 s with toothpastes with differing relative dentine abrasivity (RDA), either Toothpaste A (RDA = 90) or Toothpaste B (RDA = 200), using a toothbrush mounted in a brushing machine. The toothpastes were dispersed in water and 1% sodium carboxymethyl cellulose to give a range of toothpaste concentrations. After brushing the Knoop indent length and the enamel–dentine step were remeasured and the wear to the enamel and dentine calculated. An equation for modelling the decrease in toothpaste concentration with brushing time was found using published in vivo data and used to estimate the level of dentine wear during in vivo brushing. Results The mean enamel wear was in the range 0.03–0.11 μm and there was only a small but non-significant ( p > 0.05) increase in enamel wear with toothpaste concentration. The mean dentine wear was in the range 0.28–27.63 μm and the dentine wear increased significantly ( p < 0.05) with toothpaste concentration, with Toothpaste B giving more wear than Toothpaste A. Analysis with published in vivo data showed that 50% of dentine wear occurs in the first 20 s of brushing and that the wear approaches a plateau value with brushing time as the toothpaste concentration is reduced. Conclusions Enamel wear increases only slightly with toothpaste concentration whereas dentine wear increases with toothpaste concentration.

Caries prevention with fluoride toothpaste in children: an update

The aim was to examine recent literature and review the caries-preventive effect of fluoride toothpaste in children. Based on three comprehensive systematic reviews published in 2003, a broad search of the PubMed and Cochrane library databases was conducted for papers published 2002-2008 using "fluoride toothpaste", "fluoride dentifrice" and "fluoride dental cream" as index terms. Relevant publications were identified after assessment of their abstracts. Papers were selected if they reported a prospective controlled design with caries data reported at baseline and at the end of the study. The targeted papers were critically assessed concerning design, methodology and performance according to a pre-determined checklist. The initial search revealed 179 papers of which 15 met the inclusion criteria. There was strong evidence that daily use of fluoride toothpaste has a significant caries-preventive effect in children compared with placebo (prevented fraction 24%). The effect was boosted by supervised tooth brushing, increased brushing frequency to twice daily, and use of a toothpaste concentration of 1,500 ppm fluoride. There were few studies of high quality reporting findings from the primary dentition. There were no studies available, and therefore insufficient evidence, on when to commence brushing with fluoride toothpaste as well as on the post-brushing behaviour. The use of "adult" toothpaste in very young children seemed to increase the risk for mild dental fluorosis in low-caries communities but not in socially deprived high-caries populations. The results reinforced the outstanding role of fluoride toothpaste as an effective caries preventive measure in children.

Influence of Fluoride Concentration of Toothpaste on Remineralization of Enamel

Influence of Fluoride Concentration of Toothpaste on Remineralization of Enamel

Development of a standardized method for comparing fluoride ingested from toothpaste by 1.5‐3.5‐year‐old children in seven European countries. Part 1: Field work

To develop a standardized method for measuring the variables affecting fluoride ingestion from toothpaste in young children between the ages of 1.5 and 3.5 years, and to use the method at seven European sites. Random samples of children were invited to take part in the study. Parents who gave consent were visited at home. The children brushed their teeth using the toothpaste brand and toothbrush type currently in use. Variables measured were: type of toothpaste used, fluoride concentration of toothpaste used, weight of toothpaste used, frequency of brushing and body weight of the child. It was not possible to follow the agreed protocol in all seven countries and in three countries appropriate alternative methods were employed. There was considerable variation between countries in the variables investigated. Use of children's toothpaste ranged from 69% in Ireland to 98% in Portugal. In the Netherlands up to 60% of the children were using toothpaste containing <400 ppm F and in Finland up to 27% of children were using toothpaste containing >1200 ppm F. Over half of the children used <0.25 g of toothpaste per brushing and the majority of children brushed once or twice per day. Although adherence to the agreed protocol was not possible at all study sites there was a clear picture of considerable variation in the oral hygiene practices of young children throughout Europe.

Advising on fluoride toothpaste

Objective To describe the knowledge and practice of general dental practitioners (GDPs) working in Liverpool (where there is no milk fluoridation programme) and St Helens and Knowsley, and the Wirral (where children have fluoridated milk in schools and pre-schools) relating to the advice given for child patients regarding the use of fluoridated toothpaste. Design Data were collected via a postal questionnaire sent to all 329 GDPs working within the three areas. GDPs working in more than one of the areas and those working in specialist orthodontic or oral surgery practices were excluded. Results Two hundred and thirty-four (71%) questionnaires were completed and returned. Only 3% of dentists said that no-one in their practice gave advice on the concentration of fluoride toothpaste to be used. For caries free children under 7 years of age only 64% of GDPs gave advice concerning the concentration of toothpaste which coincided with the available clinical guidelines. Twenty eight per cent of GDPs also contradicted the guidelines by advising children under 7 with high caries to use a low fluoride toothpaste. Although 59% of GDPs in the fluoridated milk areas asked the child whether they had fluoridated milk at school, they did not appear to alter the advice given regarding the use of fluoridated toothpaste. Conclusion The study showed that a significant number of GDPs did not adhere to clinical guidelines relating to the use of fluoride toothpaste when giving advice to their child patients. For evidence-based dentistry to become a reality in this area, ways must be found to disseminate the available guidelines more fully and increase their acceptance and use by practitioners.

General dental practitioner advice regarding the use of fluoride toothpaste in two areas with a school-based milk fluoridation programme and one without such a programme.

To describe the knowledge and practice of general denta practitioners (GDPs) working in Liverpool (where there is no milk fluoridation programme) and St Helens and Knowsley, and the Wirral (where children have fluoridated milk in schools and pre-schools) relating to the advice given for child patients regarding the use of fluoridated toothpaste. Data were collected via a postal questionnaire sent to all 329 GDPs working within the three areas. GDPs working in more than one of the areas and those working in specialist orthodontic or oral surgery practices were excluded. Two hundred and thirty-four (71%) questionnaires were completed and returned. Only 3% of dentists said that no-one in their practice gave advice on the concentration of fluoride toothpaste to be used. For caries free children under 7 years of age only 64% of GDPs gave advice concerning the concentration of toothpaste which coincided with the available clinical guidelines. Twenty eight per cent of GDPs also contradicted the guidelines by advising children under 7 with high caries to use a low fluoride toothpaste. Although 59% of GDPs in the fluoridated milk areas asked the child whether they had fluoridated milk at school, they did not appear to alter the advice given regarding the use of fluoridated toothpaste. The study showed that a significant number of GDPs did not adhere to clinical guidelines relating to the use of fluoride toothpaste when giving advice to their child patients. For evidence-based dentistry to become a reality in this area, ways must be found to disseminate the available guidelines more fully and increase their acceptance and use by practitioners.