Concentration Of Test Solution Research Articles

Study of thermal shock and chemical durability of a porcelain formulated from local raw materials from Burkina Faso

This work evaluates the resistance to chemical attack and thermal shock of porcelain formulated from local raw materials from Burkina Faso for their validation in real use. A kaolinitic clay, a pegmatite and sand were used for the formulation of porcelain tiles. Some samples were shaped by casting into porous molds and sintered at a temperature of 1240 °C. The average heating rate is 10 °C/min up to the final given temperature. These porcelains were immersed to a depth of 25 mm in test solutions and kept closed at 20 °C for 12 days. Six concentrations of test solutions were prepared to perform these tests: ammonium chloride (100 g/L); hydrochloric acid (3% and 18% by volume); lactic acid (5% by volume); citric acid (100 g/L) and alkali KOH (30 g/L and 100 g/L). The test showed that these porcelains are resistant to chemical attacks with insignificant mass variations ranging from −0.030 to 0.053 wt%. The results of the thermal shock test show that formulated porcelains are resistant to the brutal variation of temperature. The results obtained qualify the porcelains for industrial and real use.

The Difference of Antioxidants Activity between Methanol Extract and Ethanol Extract of Bay Leaves (Syzygium polyanthum (Wigh) Walp) USED DPPH Method (1,1-difenil-2-pikrilhidrazil)

Antioxidant are compounds that can inhibit oxidant by reacting with reactive of free radicals to form non reactive free radicals that was relatively stable. One of the natural antioxidants derived from plants is salam leaf. Salam leaf (Syzygium polyanthum (Wigh) Walp) has the chemical content of polyphenol compounds such as flavonoids and tannin its efficacious is as antioxidants. The aims of this research is to determine whether there is a reaction on antioxidant activity of ethanol extract and methanol extract of salam leaves, as well as to know whether there is a difference of antioxidants activity between the ethanol extract and methanol extract. The extraction of salam leaves is done by maceration method using liquid extraction 70% ethanol and methanol. The ethanol extract and methanol extract of salam leaves is made by the concentration 0,06%; 0,08%; 0,10%; 0,12%; 0,16% and its antioxidants power is being tested with DPPH. The antioxidants activity is determined by DPPH method with spectrofotometry UV-Vis till gets value EC50. This value EC50 is reached through the linear regression equation which states the relationship between the concentration of test solution (x) with the antioxidant activity (y) of a series of replicate measurements. The quantitative test results show that the average value EC50 for the ethanol extract of salam leaves is 14,2168 µg/ml and methanol extract of salam leaves is 12,4338 µg/ml. From the test results of “t” test in EC50 shows significant value 0,188 > 0,05 difference between ethanol extract (α value) which means that there is no significant difference between ethanol extract and methanol extract of salam leaves.

Benefits of freeze dried arabica coffee peels (Coffea arabica) as an oral antimicrobial

Background: Oral infections are currently treated with synthetic drugs, namely antimicrobials (antibiotics and antifungals) that can cause resistant bacteria, so alternative antimicrobials from herbal groups are needed. Arabica coffee peel contains phytoche-mical compounds (polyphenols, alkaloids, saponins) which have potential as natural antibacterial. Purpose:To examine the benefits of waste of arabica coffee rind in inhibiting growth of Streptococcus mutans, Porphyromonas gingivalis and Candida albicans. Methods: This experimental laboratory with the post test only group design was started with making of freeze dried coffee peels 25%, 50% and 75%; positive control used chlorhexidine and nystatin. The antimicrobial test method was performed by the diffusion method and the growth of microbial colonies was calculated using a colony counter. Result: Antimicrobial effect increased at all concentration of test solutions to the three microbes. This shows that there is a strong positive relationship between concentration and the number of microbial colonies. The higher concentration of dried arabica coffee peels, the greater the effect of microbial killing power. The biggest effect is on C.albicans, P.gingivalis, S.mutans, consecutively. Conclusion: arabica coffee fruit skin can be used as an antimicrobial oral cavity against the growth of S.mutans, P.gingivalis and C.albicans.

Liquid Smoke of Red Fruit (Pandanus Conoideus. L.) Waste with Pyrolysis Method For Controlling Sweet Potatoes (Ipomea Batatas. L.) Pest

Pandanus conoidus, or red fruit, is a nutrient-dense native Papuan plant, whereas Ipomea batatas are the main food for the Wamena people. Biomass from red fruit seed waste can be used as liquid smoke to control Ipomea batatas . At a temperature of between 300 - 450 °C, liquid smoke is produced through a pyrolysis process. Pyrolysis produces phenolic chemicals and aldehydes that have antioxidant and antimicrobial properties (anti-bacterial and anti-fungal). The assessment of pH, acidity, and phenol concentration characterizes the quality of liquid smoke. The potential of red fruit seed waste as a liquid smoke product for biopesticides in Ipomea batatas is explored in this study. Additionally, study development aims to determine the optimal method for pyrolyzing red fruit seed waste, its characteristics, chemical content, and the way to use liquid smoke as a biopesticide in Ipomea batatas . The results showed that the liquid smoke of grade 3 red fruit seed waste contains 7 components that can be detected using a mass spectrometer. The higher the concentration of liquid smoke produced by grade 3 red fruit seeds, the higher the possibility of Cylas formicarius mortality. Cylas formicarius had the highest mortality rate (90%) when tested at a 7% test solution concentration.

ЛАБОРАТОРНЫЙ СКРИНИНГ СОРТОВ ДОННИКА БЕЛОГО ОДНОЛЕТНЕГО ПО УСТОЙЧИВОСТИ К СОЛЕВОМУ СТРЕССУ

The purpose of the research was to laboratory diagnostics of the resistance of released varieties of white sweet clover to chloride salinization in the juvenile stage of development based on the assessment of the variability of the morphometric parameters of seedlings. The object of the study was the varieties of annual white sweet clover, bred by the Samara Federal Research Center of the Russian Academy of Sciences: Povolzhsky, Srednevolzhsky and Zavolzhsky. To determine the salt tolerance of sweet clover varieties, the method of seed germination in sodium chloride solutions was used. It has been established that chloride salinization significantly reduces the germination of sweet clover seeds at a test solution concentration of 0.25 mol/l. With an increase in NaCl concentration to 0.30 mol/l in the studied varieties, the number of germinated seeds decreased by 80.2–85.9% relative to the control variant. In terms of seed germination under the influence of a stress factor, the Povolzhsky variety stood out, the Zavolzhsky variety was noted as the most resistant to the negative effects of a high degree of salinity. With chloride salinity of 0.10; 0.15 and 0.20 mol/l variety Povolzhsky provided the best morphometric parameters of seedlings. The level of growth processes at the maximum level of salinity (0.25 and 0.30 mol/l) in Zavolzhsky variety was the highest. The use of the method of laboratory assessment of salt tolerance by seed germination in saline solutions made it possible to differentiate the studied varieties of annual white sweet clover in terms of sensitivity to the impact of a stress factor at the initial stages of ontogenesis. Taking into account the high field productivity and ecological plasticity of the studied varieties, they can be recommended for use as a source material in breeding programs to create highly productive varieties resistant to chloride salinity.

In Vitro Antibacterial Effectiveness of a Naturopathic Oral Care Product on Oral Pathogens.

Currently, the prevention of periodontal diseases focuses on mechanical removal of pathogenic biofilms combined with oral antiseptics as supportive chemical antibacterial control. Due to the risk of resistance development and side effects of existing antiseptics, the interest in alternative medicine with naturopathic treatment modalities is growing in dentistry. In the present study, the antibacterial effect of the naturopathic oral care product Repha OS and some of its derivatives, based on medicinal plant extracts and essential oils, with a specific focus on added sweeteners, was investigated on periodontal pathogenic and halitosis-associated bacteria. The antibacterial efficacy was investigated by agar dilution assay. The minimum inhibitory concentration (MIC) for the bacterial species Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia and Solobacterium moorei was determined. A concentration-dependent antibacterial effect on oral bacterial species by Repha OS and its derivatives was demonstrated. For the original product, the maximum MIC was 10% of the calculated test solution concentration in agar for all examined bacterial species. The removal of essential oils reduced the antibacterial efficacy, whereas the displacement or replacement of sweeteners had almost no effect. In addition to other individual effects of the ingredients, the results of this study show that an antibacterial effect of the naturopathic oral care product on the tested oral bacterial species was achieved in vitro. In vivo, the combination of this antibacterial effect with other properties of the various ingredients may be interesting for a holistic approach in preventive dentistry.

Study on the influence of test solution concentration on the chemical corrosion resistance of ceramic tiles

Chemical resistance is one of the important evaluation factors of ceramic tiles. This article describes the method for determining the chemical resistance of ceramic tiles, and discusses the influence of acid-base solution concentration on the test results during the experiment, and proposes corresponding improvement measures.

Validation of Spectrophotometric Method for Determination of Thiamazole in Liquids by Dissolution Test for the Transdermal Form

The purpose of this work was to validate the method of UV absorption spectrophotometry for the determination of thiamazole in transdermal patches during their pharmaceutical development. According to the pharmacopoeial requirements of the Republic of Kazakhstan, dissolution test was used to assess the quality of drug product. Measuring the optical density of thiamazole solutions at a wavelength of 252 nm in the range of concentrations from 0.005 to 0.04 mg/mL showed that the optimal test solution concentration is 0.005 mg/mL. The proposed method was validated through the standard and drug recovery method for linearity, range, accuracy and precision. Optimized and validated, it demonstrated high specificity and accuracy for transdermal patch with thiamazole. The obtained results proved that the method could be used for routine analysis as well as for pharmaceutical development and quality control for thiamazole patches.

Effects of silver and zinc on tropical freshwater organisms: Implications on water quality guidelines and ecological risk assessment

Tropical freshwater ecosystems comprise valuable habitats and important biodiversity. There is an imperative need to build a framework for establishing appropriate water quality guidelines of chemical contaminants in order to safeguard freshwater ecosystems in the tropics. Silver (Ag) and zinc (Zn) are known to be toxic to freshwater organisms at elevated concentrations. However, toxicity data of tropical freshwater species for these metals are lacking. To address this issue, we first conducted standard acute toxicity tests of each of the metal using eight tropical freshwater species based on measured concentrations in test solutions. Subsequently, we tested the null hypothesis that there are no differences in species sensitivities towards Ag and Zn between tropical and temperate species by comparing their corresponding species sensitivity distributions (SSDs). The results of SSD comparisons indicated that tropical species were more acutely sensitive to their temperate counterparts to Ag, while temperate species were slightly more sensitive towards Zn. Temperate-to-tropic extrapolation factor of 5 was recommended for Ag, while acute-to-chronic ratios (ACRs) of 100 and 10 were advocated for Ag and Zn, respectively. Through comparison of taxon-specific SSDs, we observed that crustaceans were the most sensitive taxon to Ag and Zn, and thus suitable for toxicity testing and risk assessment. Based on our results, concentrations of 0.004 and 6.4 μg L−1 (at hardness of 50 mg L−1 CaCO3) were recommended as interim chronic predicted no-effect concentrations for Ag and Zn, respectively for protecting freshwater ecosystems in tropical regions.

CHROMATOGRAPHIC SYSTEM SUITABILITY TESTING: INFLUENCE OF CORRECTION FACTORS AND LATE ELUTING PEAKS OF IMPURITIES ON THE REQUIREMENT FOR THE SIGNAL-TO-NOISE RATIO (REVIEW)

Introduction. In case of methods of impurities determination quite often there are problems connected both with establishment and with check of implementation of the requirement to necessary sensitivity of a chromatographic system. Text. Influence of correction factors and late eluting peaks of impurities on the requirement for the signal-to-noise ratio (S/N) for chromatographic system suitability testing is considered. Conclusion. The recommendations are provided how to detect situations at which the requirement of S/N≥10 is insufficient for reliable detecting peaks of all impurities (related substances) and therewith how to correct concentration of test solution and/or the requirement to S/N for system suitability testing.

UJI SITOTOKSIK DENGAN METODE BRINE SHRIMP LETHALITY TEST DAN PROFIL METABOLIT SEKUNDER DARI EKSTRAK BIJI BUAH BERENUK (Crescentia cujete Linn)

ABSTRACTThe purpose of this research is to find out the profile of secondary metabolite contained in the extract of seeds (Crescentia cujete Linn.) And cytotoxic test against shrimp larvae Artemia salina Leach by BSLT method. Phytochemical tests showed that the extract be expected contained the terpenoid, alkaloid, phenolic, secondary metabolite compounds. The results of qualitative analysis with positive TLC contains phenolics. The result of identification with Spectrophotometer UV-visfor determination of α max from berenuk seeds extract consisted of 3 peaks with α max 326 nm, 258 nm, 222 nm be expected terpenoid compound, alkaloid, and phenolic. The results of toxicity test using shrimp larvae (Artemia salina leach) were divided into 4 groups: 1 control group and 3 groups of concentration series. The concentration of test solution used was 1,000, 100 and 10 μg / ml. The shrimp larvae mortality was observed after 24 hours of treatment. Based on the analysis with probit method, LC50 value of seed extract berenuk to shrimp larvae Artemia salina Leach is equal to 82,30 μg/ml. This shows that the extract of berenuk berries is toxic to shrimp larvae Artemia salina Leach, presence to terpenoid content, alkaloids and phenolics in the extract of seeds berenuk.Keywords : Secondary metabolite; Cytotoxic; BSLT.

Nutrient balancing for phytoremediation enhancement of urea manufacturing raw wastewater

Application of urea manufacturing wastewater to teak (Tectona grandis) trees, a fast growing tropical timber plants, is an environmentally-friendly and cost-effective alternative for treatment of nitrogen-rich wastewater. However, the plant growth is strongly limited by lack of phosphorus (P) and potassium (K) elements when the plants are irrigated with wastewater containing high concentration of nitrogen (N). A greenhouse experiment was conducted to optimize the efficiency of teak-based remediation systems in terms of nutrient balance. Twelve test solutions consisted of 4 levels of P (95, 190, 570, 1140 mgL−1) and 3 levels of K (95, 190, 570 mgL−1) with a constant level of N (190 mgL−1) were applied to teak seedlings every four days during the study period. Evapotranspiration rate, nutrient removal percentage, leaf surface area, dry weight and nutrient contents of experimental plants were determined and compared with those grown in control solution containing only N (N:P:K = 1:0:0). Teak seedlings grown in units with 1:0.5:1 N:P:K ratio were highly effective at nutrient removal upto 47%, 48% and 49% for N, P and K, respectively. Removal efficiency of teak plants grown in other experimental units decreased with increasing P and K concentrations in test solutions. The lowest nutrient removal and plant growth were recorded in units with 1:6:0.5 N:P:K ratio which received the highest ratio of P to K. The findings indicated that teak seedlings functioned effectively as phytoremediation plants for N-rich wastewater treatment when they were being supplied with proper concentrations of P and K.

POTENSI ANTIBAKTERI DAN ANTICANDIDA EKSTRAK ETANOL DAUN PEGAGAN (Centella asiatica (L) Urb.)

Leaves pegagan (Centella asiatica (L) Urb.) is one of the medicinal plants that have various properties. It can treat fever, antibacterial, hypo-allergenic, and central nervous system stimulants. Pegagan leaf can be used as an antibacterial because it contains flavonoids, saponins, steroids, terpenoids, and tannins. The purpose of this study was to investigate the antibacterial activity of ethanol extract of Centella asiatica leaf on the growth of Staphylococcus aureus and Escherichia coli as well as anticandida activity against the growth of Candida albicans. Simplicia Pegagan leaf extracted using remaseration methods with a solution of 70% ethanol. Antibacterial test method using the disc diffusion method (Kirby and Bauer Test). Concentration of test solution used was 20%, 40%, 60%, 80% and 100% extract in DMSO, with a positive control and a negative control chloramphenicol and Dimethyl sulfoxide (DMSO). anticandida test used agar diffusion method and Potato Dextrose agar (PDA) media. The concentration of the test solution was 20%, 40%, 60% and 100% extract in DMSO, ketoconazole as a positive control and negative control DMSO used. Diameter were analyzed using one-way ANOVA statistical test. The results showed that ethanol extracts of leaves pegagan (Centella asiatica (L) Urb.) concentration of 60%, 80%, and 100% have antibacterial activity against Staphylococcus aureus and Escherichia coli. Anticandida test concentration of ethanol extract of Centella asiatica leaf provides the greatest inhibition zone is a concentration of 60%. The ethanol extract of leaves pegagan (Centella asiatica (L) Urb.) qualitatively contains flavonoids, saponins and tannins.

직독식 측정기 PID와 능동식 시료채취기에 의한 GC-FID 정량분석법의 VOCs 농도 비교 연구

Objectives: Direct-reading instrument(Photoionization detectors, PID) and quantitative analysis using active type air sampling (Gas chromatography-flame ionization detector, GC-FID) were tested to evaluate their ability to detect volatile organic compounds(VOCs) in a semiconductor manufacturing plant. Methods: The organic compounds used were acetone and ethanol which are normally used as cleaning solutions in the semiconductor manufacturing. The evaluation was based on the preparation of test solutions of known acetone and ethanol concentration in a chamber(<TEX>$600{\times}600{\times}1150mm$</TEX>). Samples were prepared that would be equivalent to 5~100 ppm for acetone and 10~ 200 ppm ethanol. GC-FID and PID were evaluated simultaneously. Quantitative analysis was performed after sampling and the direct-reading instrument was checked using real-time data logging. Results: Positive correlations between PID and GC-FID were found for acetone and ethanol at 0.04~2.4% for acetone(TLV: 500 ppm) and 0.1~8.3% for ethanol(TLV: 1000 ppm). When the sampling time was 15 min, concentration of test solution was the most similar between measurement methods. However, the longer the sampling time, the less similar the results. PID and GC-FID had similar exposure patterns. Conclusions: The results indicate that PID and GC-FID have similar exposure pattern and positive correlation for detection of acetone and ethanol. Therefore, PID can be used for exposure monitoring for VOCs in the semiconductor manufacturing industry. This study has significance in that it validates measuring occupational exposure using a portable device.

Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs

Salinity is an important characteristic of the aquatic environment. For aquatic organisms it defines the habitats of freshwater, brackish water, and seawater. Tests of the toxicity of chemicals and assessments of their ecological risks to aquatic organisms are frequently performed in freshwater, but the toxicity of chemicals to aquatic organisms depends on pH, temperature, and salinity. There is no method, however, for testing the salinity dependence of toxicity to aquatic organisms. Here, we used medaka (Oryzias latipes) because they can adapt to freshwater, brackish water, and seawater. Different concentrations of embryo-rearing medium (ERM) (1x, 5x, 10x, 15x, 20x, and 30x) were employed to test the toxicity of silver nanocolloidal particles (SNCs) to medaka eggs (1x ERM and 30x ERM have osmotic pressures equivalent to freshwater and seawater, respectively). In six-well plastic plates, 15 medaka eggs in triplicate were exposed to SNCs at 10 mg/L−1 in different concentrations of ERM at pH 7 and 25 °C in the dark. We used a dissecting microscope and a micrometer to measure heart rate per 15 sec and eye diameter on day 6 and full body length of the larvae on hatching day (section 4). The embryos were observed until hatching or day 14; we then counted the hatching rate every day for 14 days (section 4). To see silver accumulation in embryos, we used inductively coupled plasma mass spectrometry to measure the silver concentration of test solutions (section 5) and dechorionated embryos (section 6).The toxicity of the SNCs to medaka embryos obviously increased with increasing salinity. This new method allows us to test the toxicity of chemicals in different salinities.

Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs.

Salinity is an important characteristic of the aquatic environment. For aquatic organisms it defines the habitats of freshwater, brackish water, and seawater. Tests of the toxicity of chemicals and assessments of their ecological risks to aquatic organisms are frequently performed in freshwater, but the toxicity of chemicals to aquatic organisms depends on pH, temperature, and salinity. There is no method, however, for testing the salinity dependence of toxicity to aquatic organisms. Here, we used medaka (Oryzias latipes) because they can adapt to freshwater, brackish water, and seawater. Different concentrations of embryo-rearing medium (ERM) (1x, 5x, 10x, 15x, 20x, and 30x) were employed to test the toxicity of silver nanocolloidal particles (SNCs) to medaka eggs (1x ERM and 30x ERM have osmotic pressures equivalent to freshwater and seawater, respectively). In six-well plastic plates, 15 medaka eggs in triplicate were exposed to SNCs at 10 mg/L(-1) in different concentrations of ERM at pH 7 and 25 °C in the dark. We used a dissecting microscope and a micrometer to measure heart rate per 15 sec and eye diameter on day 6 and full body length of the larvae on hatching day (section 4). The embryos were observed until hatching or day 14; we then counted the hatching rate every day for 14 days (section 4). To see silver accumulation in embryos, we used inductively coupled plasma mass spectrometry to measure the silver concentration of test solutions (section 5) and dechorionated embryos (section 6).The toxicity of the SNCs to medaka embryos obviously increased with increasing salinity. This new method allows us to test the toxicity of chemicals in different salinities.

Potentiometric and pHmetric Studies of Paracetamol

Acid-base titration of paracetamol in nonaqueous solvents was done. Procedure was followed for titration of paracetamol in different media like acetic acid, pyridine, dimethlformamide and ethyl alcohol with standard perchloric acid in glacial acetic acid, sodium ethoxide in ethyl alcohol using plantinum-calomel as well as glass-calomel electrode system. The equivalence point was located as accurately as possible by a differential graph of ΔE/ΔV or ΔpH/ΔV against V and concentration of test solution was computed. The acid-base titration of paracetamol is rapid and reproducible, and permits its determination in medicinal sample. The electrode systems vary with the solvent employed. The platinum-calomel electrode system is suitable where the solvent is glacial acetic acid in this case perchloric acid in glacial acetic acid is the titrant while the glass-calomel electrode system is suitable where the solvent is either pyridine, an alcohol or dimethyiformamide, the titrant consists of sodium ethoxide in ethyl alcohol.

Simulation of the influence of test factors on the corrosion of metal materials tested for the aggressive influence of components of the industrial atmosphere in a salt spray chamber

Simulation of the influence exercised by the main test factors (humidity, temperature, and aggressive components of the atmosphere) on the corrosion of metal materials was performed with the purpose to develop methods for corrosion testing of materials, junctions, and products in order to estimate the performance level of devices under all climatic operating conditions. Theoretical values of the acceleration coefficients were obtained for each test factor. Values of the test parameters were calculated, namely, concentrations of aggressive substances in a test chamber. Taking into account the earlier work on simulation of the influence of aggressive components in the industrial atmosphere on metal materials in a salt spray chamber, the concentrations of test solutions were calculated.

Tailoring, physicochemical characterization, antibacterial and DNA binding mode studies of Cu(II) Schiff bases amino acid bioactive agents incorporating 5-bromo-2-hydroxybenzaldehyde

Five novel Cu(II) complexes derived from the condensation between 5-bromosalicylaldehyde (bs) and α-amino acids (L-alanine, l-phenylalanine, L-aspartic acid, L-histidine and L-arginine) were synthesized and characterized by their elemental analyses, thermogravimetric analysis, IR, mass and electronic spectra, conductance and magnetic measurements. Moreover, the stoichiometry and the stability constants of the prepared complexes have been determined spectrophotometrically using continuous variation and molar ratio methods. The obtained results indicated that the Schiff bases of the amino acids: L-alanine, L-phenylalanine, L-histidine and L-arginine behave as tridentate ligands. The ligands are coordinating with the Cu(II) via azomethine nitrogen, deprotonated carboxylate oxygen and phenolic oxygen. However, in the case of L-aspartic, the ligand acts as tetradentate due to the coordination of the second carboxylate group. Based on the studies of magnetic moments and electronic spectra, a square planar geometry has been proposed for all Cu(II) complexes except bromsalicylaldehydine aspartate complex which has a distorted tetrahedral structure. The representative Schiff bases and their Cu(II) complexes were tested in vitro for their antibacterial activity against two Gram-positive bacteria (Micrococcus luteus and Bacillus cereus) and one Gram-negative bacteria (Pseudomonas aeruginosa). All the complexes showed activity against the organisms more than the free Schiff base ligands and the activity increases with the increase in concentration of test solution containing the new complexes. Moreover, the interaction of the prepared Schiff base amino acid Cu(II) complexes with calf thymus DNA has been investigated by absorption spectra, viscosity and gel electrophoresis measurement and the mode of CT-DNA binding to the complexes has been explored. The investigated complexes exhibit cooperative binding and presumably intercalate into DNA and follow the trend: bsarc>bshc>bsalc>bsphalc>bsasc.

Application of Micro-kinetic Chromogenic Method in Endotoxin Quantitative Detection of Hydroxypropyl-β-cyclodextrin

The kinetic chromogenic assay with Limulus amoebocyte lysate to detect the bacterial endotoxin in Hydroxypropyl-β-cyclodextrin for injection is investigated in this paper. The test for interfering factors is performed to study the applicability of the kinetic chromogenic assay for detection of bacterial endotoxin in Hydroxypropyl-β-cyclodextrin for injection. The results suggest that r&amp;gt;0.980 while the range is between 50-0.005EU/ml. Besides, when concentration of test solution is 1mg/ml for Hydroxypropyl-β-cyclodextrin, there is no interference between Limulus amebocyte lysate and bacterial endotoxin. The kinetic chromogenic assay can be used to detect the bacterial endotoxin in Hydroxypropyl- -β-cyclodextrin.