Solvent Content Research Articles

Polyphenol Extraction from Musa corniculata Peel Using Microwave Assisted Extraction (MAE) Method with Ethanol Solvent

Banana peel (Musa corniculata) is one of the superior plants in Indonesia which is rich in polyphenolic compounds as a source of antioxidants. The potential of polyphenolic compounds as antioxidants can be used as an alternative to reduce banana peel waste. This study aims to determine the total content of polyphenols and antioxidant activity of horn banana peel extract. Extraction used the Microwave Assisted Extraction (MAE) method with ethanol solvent and the variables were extraction time (4, 7, and 10 minutes), solvent concentration (50, 73 and 96 %), ratio of simplicia mass to solvent volume (1:12, 1 :8, 1:6 g/mL) with a microwave power of 150 Watt. Determination of polyphenol content using the Folin-Ciocalteau method and the ability of polyphenols as antioxidants were analyzed by DPPH test. This study used the Design Expert 13 Response Surface Methodology software, the Box Behnken type, to determine the extraction parameters for the total polyphenol content. The highest total polyphenol content was obtained at 354.02 mg GAE/g from the combination of parameters 50% solvent concentration, 10 minutes extraction time, and the ratio of horn banana peel powder to 1:8 g/mL solvent.

3D GeoRemediation: A Digital Hydrogeophysical–Chemical Clone and Virtual Hydraulic Barrier with Groundwater Circulation Wells (GCWs) for Groundwater Remediation

Identification of contamination sources and delineation of plumes in the geological environment stand as pivotal elements in reconstructing the conceptual site model (CSM) and devising remediation strategies tailored to specific physicochemical traits. This study endeavors to showcase the capabilities of a 3D digital interface, seamlessly integrating multi-source data, to elucidate site-specific contamination dynamics and steer the implementation of remediation strategies harmoniously aligned with the ethos of remediation geology. In a site historically marred by chlorinated solvent contamination, the digitization of stratigraphic, piezometric, chemical, and membrane interface probe (MIP) data underpins geomodeling endeavors and yields a meticulously crafted, data-driven CSM. The hydrogeochemical and hydrogeophysical data were interpolated to build a volumetric, digital 3D model illustrating data-driven elements. The comprehensive 3D clone adeptly delineates secondary contamination sources and renders visible the contamination plume within a georeferenced framework, mirroring the nuanced interplay of stratigraphic nuances and groundwater path. A data-centric approach to modeling facilitates the design of the first hydraulic virtual barrier leveraging groundwater circulation well (GCW) technology, its geometry finely attuned to intercept the contamination plume originating from source dissolution and aligning with preferential groundwater flow trajectories. Conventional hydrochemical monitoring and multilevel sampling substantiate the discernible reduction in chlorinated solvent concentrations across various depths within the aquifer horizon, affirming the efficacy of GCWs in their virtual barrier configuration. The findings highlight the effectiveness and limited groundwater consumption of the virtual barrier compared to the on-site pump-and-stock system. This research underscores the potency of a multi-faceted evidence-driven puzzle in conceptualizing contamination mechanisms within the geological milieu, thereby fostering the application of cutting-edge, effective, and sustainable remediation strategies.

Investigation of Antibody Tolerance in Methanol for Analytical Purposes: Methanol Effect Patterns and Molecular Mechanisms.

The extraction of targets from biological samples for immunoassays using organic solvents, such as methanol, is often necessary. However, high concentrations of organic solvents in extracts invariably lead to instability of the employed antibody, resulting in poor performance of the immunoassay. Evaluating the tolerance ability and exploring the molecular mechanisms of antibody tolerance in organic solvents are essential for the development of robust immunoassays. In this work, 25 monoclonal antibodies and methanol are utilized as models to address these questions. A novel protocol is initially established to precisely and rapidly determine antibody tolerance in methanol, identifying two distinct methanol effect patterns. Through a detailed investigation of the structural basis, a novel hypothesis regarding methanol effect patterns is proposed, termed "folding-aggregation," which is subsequently validated through molecular dynamics simulations. Furthermore, the investigation of sequence basis reveals significant differences in residue types within the complementarity-determining regions and ligand-binding residues, distinguishing the two antibody methanol effect patterns. Moreover, the methanol effect patterns of the antibodies are defined by germline antibodies. This work represents the first exploration of antibody methanol effect patterns and associated molecular mechanisms, with potential implications for the discovery and engineering of tolerant antibodies for the development of robust immunoassays.

Effect of Some Technological Factors of Extraction on Total Lentinan Content in Sapa Shiitake Mushroom Extract

In Vietnam, the source of shiitake mushroom is very abundant and grown in many provinces/cities, the total yield reaches hundreds of thousands of tons/per year, mainly serving the demand of domestic food processing, and did not develop into medicinal mushrooms yet. Among them, Sapa shiitake mushroom is being widely cultivated in Sapa-Vietnam, and is a raw material with great potential for lentinan exploitation. Until now, researches on extracting and obtaining lentinan in Vietnam are still limited. Those are reasons to carry out this research. The effects of solvent in combination with assistance of ultrasound wave in lentinan extracting capability in Sapa shiitake mushroom were studied. Before carrying out extracting of lentinan; The dried fruit bodies of Sapa shiitake mushroom was crushed in to 1mm. Five extracting parameters include concentration of Na0H solvent (%), proportion of Na0H solvent and raw material (v:w), extracting temperature, ultrasound time and ultrasound intensity were carried out. An extraction without the use of ultrasound for 180 min was control sample. Total lentinan content was obtained during the extracting process. The results indicated that shiitake mushrooms were extracted by using 0.35% Na0H solvent, the rate of Na0H solvent and mushrooms was 12:1 (v:w), intensity of ultrasound was 58 W/cm2, frequency was 20 kHz, extracting temperature was 65 °C, time of extraction was 6 min gave total lentinan content 1.63 times higher than the control.

Controllable Supramolecular Aggregation of Hydroazaheptacene Tetraimides and Derivatives in Nonpolar and Polar Solvents.

Rational control of the supramolecular aggregation of π-conjugated molecules plays an important role in determining their optoelectronic properties and applications. Here, we report a systematic study of the factors, including solvent polarity, concentration, and surfactants, that affect the aggregation behavior of a brominated hydroazaheptacene tetraimide (HATI) and its thiophene-substituted derivative, Th-HATI, as near-infrared fluorophores, in both nonpolar and polar solvents. The thermal stability of the aggregates is also studied by monitoring their optical absorption against temperature change. Our results indicate that the aggregation of HATI is highly sensitive to the solvent polarity. Moreover, the average aggregation number of HATI inside the colloidal nanoparticles formed in aqueous media can be controlled by surfactants. The substitution of the bromo groups in HATI by thiophene units induces a slight blue shift of the optical absorption, enhanced crystallinity, distinct aggregation behavior in both nonpolar and polar solvents, and improved thermal stability. The multifacet understanding of the supramolecular aggregation of these systems may offer insight for other π-conjugated molecular chromophores with various optoelectronic properties and applications.

Oxime Palladacycle Supported on Magnetic Meoporous Silica: An Efficient Catalyst for Copper‐Free Acyl Sonogashira Reaction

AbstractThe catalytic activity of the supported bis(oxime palladacycle) catalyst was evaluated in the acyl Sonogashira reaction. The reaction conditions, such as solvent, base, and palladium content, were screened to achieve the best catalytic performance. Tetrahydrofuran (THF) solvent, triethylamine (Et3N) base, and the reaction temperature of 110 °C were found to be ideal for achieving high yields of the desired products. Furthermore, a catalyst loading of only 0.22 mol% palladium was sufficient to achieve good conversion. Broad ranges of aryl acetylenes and benzoyl chlorides were successfully coupled to give the desired acetylenic ketones. The reaction appeared to proceed efficiently using a small amount of catalyst. The magnetic property of the catalyst allowed for easy separation using an external magnet. The catalyst was stable and reused for multiple reaction runs without a significant deactivation. After seven cycles, the catalyst maintained over 90 % of its initial catalytic activity.

Ultrasonic-assisted extraction of total flavonoids from Zanthoxylum bungeanum residue and their allelopathic mechanism on Microcystis aeruginosa

Water eutrophication has emerged as a pressing concern for massive algal blooms, and these harmful blooms can potentially generate harmful toxins, which can detrimentally impact the aquatic environment and human health. Consequently, it is imperative to identify a safe and efficient approach to combat algal blooms to safeguard the ecological safety of water. This study aimed to investigate the procedure for extracting total flavonoids from Z. bungeanum residue and assess its antioxidant properties. The most favorable parameters for extracting total flavonoids from Z. bungeanum residue were a liquid–solid ratio (LSR) of 20 mL/g, a solvent concentration of 60%, an extraction period of 55 min, and an ultrasonic temperature of 80 °C. Meanwhile, the photosynthetic inhibitory mechanism of Z. bungeanum residue extracts against M. aeruginosa was assessed with a particular focus on the concentration-dependent toxicity effect. Z. bungeanum residue extracts damaged the oxygen-evolving complex structure, influenced energy capture and distribution, and inhibited the electron transport of PSII in M. aeruginosa. Furthermore, the enhanced capacity for ROS detoxification enables treated cells to sustain their photosynthetic activity. The findings of this study hold considerable relevance for the ecological management community and offer potential avenues for the practical utilization of resources in controlling algal blooms.

A lipase from Lacticaseibacillus rhamnosus IDCC 3201 with thermostability and pH resistance for use as a detergent additive

Lipases are important biocatalysts and ubiquitous in plants, animals, and microorganisms. The high growth rates of microorganisms with low production costs have enabled the wide application of microbial lipases in detergent, food, and cosmetic industries. Herein, a novel lipase from Lacticaseibacillus rhamnosus IDCC 3201 (Lac-Rh) was isolated and its activity analyzed under a range of reaction conditions to evaluate its potential industrial application. The isolated Lac-Rh showed a molecular weight of 24 kDa and a maximum activity of 3438.5 ± 1.8 U/mg protein at 60 °C and pH 8. Additionally, Lac-Rh retained activity in alkaline conditions and in 10% v/v concentrations of organic solvents, including glycerol and acetone. Interestingly, after pre-incubation in the presence of multiple commercial detergents, Lac-Rh maintained over 80% of its activity and the stains from cotton were successfully removed under a simulated laundry setting. Overall, the purified lipase from L. rhamnosus IDCC 3201 has potential for use as a detergent in industrial applications.Key points• A novel lipase (Lac-Rh) was isolated from Lacticaseibacillus rhamnosus IDCC 3201• Purified Lac-Rh exhibited its highest activity at a temperature of 60 °C and a pH of 8, respectively• Lac-Rh remains stable in commercial laundry detergent and enhances washing performance

Molecular Drivers of Aging in Biomolecular Condensates: Desolvation, Rigidification, and Sticker Lifetimes

Biomolecular condensates are dynamic intracellular entities defined by their sequence- and composition-encoded material properties. During aging, these properties can change dramatically, potentially leading to pathological solidlike states, the mechanisms of which remain poorly understood. Recent experiments reveal that the aging of condensates involves a complex interplay of solvent depletion, strengthening of sticker links, and the formation of rigid structural segments such as beta fibrils. In this study, we use various coarse-grained models to investigate how solvent expulsion, biopolymer chain rigidity, and the lifetimes of sticker contacts influence the viscoelastic properties and aging dynamics of condensates. We find that the rigidity of the biopolymer backbone is essential for replicating the predominant elastic behavior observed in experiments. In contrast, models using fully flexible chains—an assumption common in simulations of intrinsically disordered proteins—fail to exhibit a dominant elastic regime. We also demonstrate that altering the solvent content within condensates affects the crossover between storage and loss moduli. This suggests that desolvation plays a significant role in condensate aging by promoting the transition from a viscous to an elastic state. Furthermore, the lifetime of sticker pairs profoundly influences the mature state of the condensates; short-lived stickers lead to a Maxwell fluid behavior, while longer-lived, irreversibly cross-linked stickers result in solidlike properties, consistent with the Kelvin-Voigt model. Finally, by incorporating the chain rigidification, desolvation, and sticker pair formation into a nonequilibrium dynamic aging simulation, we show the molecular mechanism of forming solid shells around the condensate surfaces observed in a recent experimental report. Published by the American Physical Society 2024

The Effect of Different Ethanol Concentrations as The Extraction Solvent on Total Flavonoid Content of White Mulberry Leaves Extracts (Morus alba L.)

White mulberry leaves (Morus alba L.) contain flavonoid which empirically have many health benefits, such as reducing fever, relieving headaches and throats, coughs, dysentery, digestive disorders, flu, and according to research it has antioxidant activity. Many factors effect the total flavonoid content, one of which is the concentration of the extraction solvent. The purpose of the research was to determine the effect of different ethanol concentrations as the extraction solvent on total flavonoid content of white mulberry (Morus alba L.) leaves extract. White mulberry leaves (Morus alba L.) were extracted using the maceration method, the extract was tested for phytochemical screening and thin layer chromatography using chloroform: ethyl acetate (6: 4) as the eluent. The resulting spots were observed visually and with λ 254 nm and λ 366 nm UV light. Determination of flavonoid content by UV-Vis spectrophotometry using the AlCl3 colorimetric method at a maximum wavelength of 415 nm. The research results showed that the yield of 50% and 96% ethanol extract of white mulberry leaves (Morus alba L.) was 17.42 % and 11.04 %. Phytochemical screening showed that both extracts positively contained flavonoids. The presence of flavonoids was confirmed using a thin layer chromatography test, resulting in an average Rf value for 50% and 96% ethanol extracts are 0.71 and 0.70, close to quercetin Rf standard is 0,73 as comparison. The total flavonoid content obtained from the 50% ethanol extract was 36.0902 mgQE/g and the 96% ethanol extract was 56.4699 mgQE/g. The conclusions are the concentration of the extraction solvent effect the yield and total flavonoid content.

The Utilization of Chicken Egg White Waste-Modified Nanofiber Membrane for Anionic Dye Removal in Batch and Flow Systems: Comprehensive Investigations into Equilibrium, Kinetics, and Breakthrough Curve.

This study investigated the use of chicken egg white (CEW) waste immobilized on weak acidic nanofiber membranes for removing the anionic acid orange 7 (AO7) dye in batch and continuous flow modes. Different experiments were conducted to evaluate the effectiveness of CEW-modified nanofiber membranes for AO7 removal, focusing on CEW immobilization conditions, adsorption kinetics, and thermodynamics. The CEW-modified nanofiber membrane (namely NM-COOH-CEW) exhibited a maximum AO7 adsorption capacity of 589.11 mg/g within approximately 30 min. The Freundlich isotherm model best represented the equilibrium adsorption data, while the adsorption kinetics followed a pseudo-second-order rate model. Breakthrough curve analysis using the Thomas model and the bed depth service time (BDST) model showed that the BDST model accurately described the curve, with an error percentage under 5%. To investigate AO7 elution efficiency, different concentrations of organic solvents or salts were tested as eluents. The NM-COOH-CEW nanofiber membrane exhibited promising performance as an effective adsorbent for removing AO7 dye from contaminated water.

Total polyphenol content and antioxidant activity of ethanolic propolis extracts

Propolis is a beekeeping by-product with a high content of polyphenols that manifests antioxidant action. In this article was conducted the comparative study of the obtained results regarding the total polyphenol content of propolis extracts and their antioxidant activity depending on several factors: solvent concentration, extraction method. The total polyphenol content was determined by the Folin-Ciocalteu method and the antioxidant activity by the DPPH method. The analysis of the data revealed that the total polyphenol content and antioxidant activity is largely depended by the solvent concentration, optimal for obtaining ethanolic extracts of propolis with a high content of polyphenols, being ethyl alcohol with a concentration of 70%.

Infrared radiation suppression and mechanical properties characterization of aluminum/glass/polysiloxane coatings

Abstract The coatings (polysiloxane, flake aluminum, and glass powder) mechanical and infrared properties were analyzed in detail. The hardness, adhesion, surface roughness, SEM, and infrared thermal imager were used to evaluate the infrared radiation suppression and mechanical performance. The influences of coatings structure (pigment/adhesive ratio, function filler, and solvent content) on infrared emissivity were analyzed through theory and experiment. The coating hardness increased as the temperature increased due to the decomposition of the adhesive. The adhesion analysis showed an “M” type trend due to the functional powder and structure of the coating. Moreover, the coating infrared radiation suppression was also analyzed after thermal and chilling shock resistance.

Full extraction of asbuton bitumen for binder replacement in warm mix asphalt

Abstract Asbuton can be utilized as an optional binder in asphalt mixture by semi-extraction or full extraction. Full extraction of asbuton by using solvent can maximize the content of bitumen while discarding the mineral. However, different solvent content influenced the asbuton bitumen properties. Thus, this study evaluated the effect of different percentages of toluene solvent on the full extraction. Four different percentages of solvent content 1 to 4 % of toluene were used in this study. The asbuton bitumen was then tested for penetration, softening point, flash point, specific gravity, and thin film oven test. The asbuton bitumen was then used to design warm mix asphalt (WMA). Based on the Marshall mix design, the optimum bitumen content (OBC) is 6.25 %. The WMA with full extraction asbuton bitumen fulfilled the Marshall requirement by stability of 1374 kg, and 96.97% retained stability. It also produced 3.57 mm flow with 3.46 % void in mix, 15.08 % void mineral aggregatte, and 76.41 of void filled bitumen. As a result, it is discovered that full extraction asbuton can serving as the WMA binder replacement.

Standardization of Phomopsis sp. extract and prediction of anti breast cancer activity from the main compound through molecular docking

Abstract Endophytic fungi isolated from Indonesian soursop (Annona muricata) leaves, which have the potential to treat breast cancer, have been successfully isolated since 2016. Identification based on the Internal Transcribed Spacer (ITS) gene revealed that fungi close to Phomopsis sp. The fungi were stored, subcultured, and extracted with ethyl acetate for in vitro and in vivo studies. To be registered as a medicinal ingredient and meet the requirements of the Indonesian Food and Drug Supervisory Agency (BPOM, Badan Pengawas Obat dan Makanan), it is necessary to ensure the standardization, safety, and quality of the Phomopsis extract. The results showed that the Phomopsis extract met the BPOM requirements for organoleptic, water, and solvent content, microbial and heavy metal contamination, total aflatoxin, and metabolite content. Analysis of the Phomopsis extract content also showed that the compounds in the two production batches were stable, with the highest relative abundance of 7-hydroxycoumarin. In silico studies with molecular docking show that the affinity energy of 7-hydroxycoumarin docking with the estrogen receptor alpha (ERα) and thymidine kinase 1 (TK1) has a lower negativity value than the comparison ligand (-6.7 and -6.8 kcal/mol).

Extraction yield and properties of biogenic silica from palm oil fuel ash: effect of re-ashing pretreatment and solvent concentration

Abstract Pretreatments and extraction conditions can affect the yield and characteristics of biogenic silica (biosilica) from palm oil fuel ash (POFA). This study aimed to examine how reashing pretreatment and varying NaOH concentrations influence the yield and properties of biosilica extracted from POFA by a simple sol-gel method. Re-ashing pretreatment was conducted using a furnace at a temperature of 600°C for 2h. All samples were leached using a citric acid solution. Extraction process was carried out using NaOH solutions with concentrations of 6%, 8%, and 10%). The results showed that re-ashing pretreatment and concentrations of NaOH significantly influenced (p<0.05) the extraction yield, color, crystallinity, and specific surface area of POFA-derived biosilica. Re-ashing pretreatment in combination with a 10% NaOH solution produced the highest extraction yield of biosilica (17.25% w/w) with a bright white color (L=91.83; WI=91.32), SiO2 concentration of 94.68%, crystallinity of 50.10%, and specific BET surface area of 390.80m2/g. XRD patterns confirmed the amorphous nature of POFA-derived biosilica. FTIR spectra revealed the presence of silica functional groups in all samples. This study demonstrated the important roles of re-ashing pretreatment and NaOH concentration in producing POFA-derived biosilica with better yield and properties that could broaden its potential applications.

Synthesizing of cobalt oxide nanofibers and investigation of changes in synthesizing parameters on the final product

AbstractIn this study, the best conditions for synthesizing the cobalt oxide nanofibers using the electrospinning method was investigated. For this purpose, the effect of various factors such as solvent, calcination temperature, and polymer concentration on the crystal structure, morphology, and size of the synthesized samples was evaluated. An extensive characterization of the products was performed using x‐ray diffractometry, scanning electron microscopy, ultraviolet‐visible spectroscopy, and photoluminescence. X‐ray diffractometry results of fabricated samples at different temperatures confirm cobalt oxide synthesis. Scanning electron microscopy analysis shows that the best fibers are synthesized from a polymer solution prepared at room temperature with a dual solvent of deionized water and ethanol under a calcination temperature of 600 °C. Also, some shifts were observed by examining the optical properties using ultraviolet‐visible and photoluminescence spectroscopy. These results show that the proper selection of reaction conditions in the electrospinning method can be very effective for the synthesis of cobalt oxide fibers with morphology in nanometer sizes.

Laboratory evaluation of cutback bitumen modified with cup lump rubber

Abstract This research introduces a novel approach to addressing challenges in conventional asphalt used for road construction by incorporating cup lump rubber into cutback bitumen. The study investigates the viscosity and flash point of the resulting cutback bitumen to identify the optimal solvent percentage for achieving a lower mixing and compacting temperature range. Various diluent percentages were tested, ranging from 40% to 50%, across temperatures from 30°C to 70°C. Results indicate that higher diluent percentages lead to decreased viscosity in the blended bitumen. The most efficient solvent content for producing cutback bitumen-modified cup lump rubber is identified at 45% and 50% by weight of the bitumen, offering a reduced mixing and compaction temperature range. However, higher diluent content increases volatility potential and reduces the flash point. Additionally, toxicity testing reveals that lead and zinc are consistently present in cup lump rubber-infused samples, albeit below hazardous waste limits set by TCLP. The absence of lead in unmodified cutback bitumen underscores its compliance with environmental safety standards. These findings underscore the minimal environmental impact of this innovative modified bitumen formulation.

Enhancing the Nutritional Profile of Crataegus monogyna Fruits by Optimizing the Extraction Conditions

Crataegus monogyna (CM) fruits are highly regarded for their rich nutritional content, boasting elevated levels of various beneficial secondary metabolites like total polyphenols, including anthocyanins, and ample amounts of ascorbic acid and antioxidant activity. Despite the acknowledged benefits of CM fruits, researchers have directed more attention toward its leaves and flowers. Consequently, the current research attempts to optimize extraction techniques for CM fruit using a multifaceted approach involving varied durations, temperatures, and concentrations of ethanol solvent to isolate the diverse range of bioactive components present effectively. High-performance liquid chromatography coupled with a diode array detector (HPLC-DAD) is employed for the identification and quantification of polyphenolic compounds. According to the results, by following the optimum extraction parameters (50% ethanolic solvent, 50 °C extraction temperature, and 60 min extraction time), the total polyphenol content can be increased up to 410%, reaching 55.59 mg gallic acid equivalents/g. Using 50% ethanolic solvent, 80 °C extraction temperature, and extraction time of 90 min, the total anthocyanin content can be enhanced by more than 560%, reaching a quantity of 51.83 μg cyanidin equivalents/g. Moreover, the antioxidant activity of CM fruit extracts can reach 415.95 μmol ascorbic acid equivalents (AAE)/g dw (by FRAP method), using 50% ethanolic solvent, 50 °C extraction temperature, and 60 min extraction time, and 270.26 μmol AAE/g dw (by DPPH method) and 1053.28 mg/100 g dw ascorbic acid content, using 50% ethanolic solvent, 80 °C extraction temperature, and 90 min extraction time. This comprehensive study seeks to augment the already substantial content of bioactive compounds found in CM, resulting in an extract with promising applications across the pharmaceutical, food, and cosmetics industries.

New Pro-ecological Alkyd Binder for Moulding Sands with Limited Solvent Content

New Pro-ecological Alkyd Binder for Moulding Sands with Limited Solvent Content