Sodium Alginate Concentration Research Articles

Stable O/W emulsions by combining Pluronic L64 and Sodium Alginate

Emulsions play a pivotal role in many industrial fields, including food, pharmaceutics, and cosmetics. This paper explores the simultaneous effects of varying concentrations of Pluronic and Sodium Alginate (Alg), with particular attention to the resulting stability and the rheological properties of the incoming oil-in-water emulsions. Pluronics are used as amphiphilic molecules to stabilize the oil droplet interface, Alg is instead used to increase the viscosity of the suspending medium in a way to slow down droplet coalescence. High shear mixing is used to prepare the emulsions, and rheology and optical microscopy are employed to assess their microstructure. By optimizing both Alg and Pluronic concentrations, emulsions stable up to 90 days are achieved.

Effects of Incorporating Ionic Crosslinking on 3D Printing of Biomass-Fungi Composite Materials.

Biomass-fungi composite materials primarily consist of biomass particles (sourced from agricultural residues) and a network of fungal hyphae that bind the biomass particles together. These materials have potential applications across diverse industries, such as packaging, furniture, and construction. 3D printing offers a new approach to manufacturing parts using biomass-fungi composite materials, as an alternative to traditional molding-based methods. However, there are challenges in producing parts with desired quality (for example, geometric accuracy after printing and height shrinkage several days after printing) by using 3D printing-based methods. This paper introduces an innovative approach to enhance part quality by incorporating ionic crosslinking into the 3D printing-based methods. While ionic crosslinking has been explored in hydrogel-based bioprinting, its application in biomass-fungi composite materials has not been reported. Using sodium alginate (SA) as the hydrogel and calcium chloride as the crosslinking agent, this paper investigates their effects on quality (geometric accuracy and height shrinkage) of 3D printed samples and physiochemical characteristics (rheological, chemical, and texture properties) of biomass-fungi composite materials. Results show that increasing SA concentration led to significant improvements in both geometric accuracy and height shrinkage of 3D printed samples. Moreover, crosslinking exposure significantly enhanced hardness of the biomass-fungi mixture samples prepared for texture profile analysis, while the inclusion of SA notably improved cohesiveness and springiness of the biomass-fungi mixture samples. Furthermore, Fourier transform infrared spectroscopy confirms the occurrence of ionic crosslinking within 3D printed samples. Results from this study can be used as a reference for developing new biomass-fungi mixtures for 3D printing in the future.

3D printing of glycerol-mediated alginate hydrogels with high strength and stiffness

As a biomass material with biodegradability and biocompatibility, sodium alginate (SA) is a good candidate for constructing hydrogels for tissue-mimicking and biomedical scaffold fabricating through extrusion-based 3D printing technology. However, the mechanical strength and stiffness of alginate hydrogels are still not comparable with biological tissues such as tendons and the printability of SA solutions is often poor. Here, a novel strategy for 3D printing of alginate hydrogels with high mechanical performance is developed by using glycerol as a co-solvent for SA solutions. The addition of glycerol (GL) enables the formation of a homogenous SA/GL solution with a high solid content of 12–20 wt.% and endows crosslinked SA hydrogels with high stretchability. By applying uniaxial stretches, hydrogel filaments prepared with concentrated SA/GL solutions reveal a high tensile strength of 36.6–161.3 MPa, Young's modulus of 59.2–1964.2 MPa, and elongation at break of 8.5 %–106.2 % due to the high orientated and closely packed SA chains. SA/GL solutions become more solid-like with increasing SA concentration, and the solution with a solid content of 16 wt.% exhibits optimal 3D printability because of the appropriate rheological properties and thixotropic behavior. By designing the deforming-and-fixing process, 3D printed high-strength alginate hydrogels with complex structures are prepared, broadening the application of alginate hydrogels in load-bearing and biomedical fields.

Optimization of encapsulation parameters for sodium alginate capsules: A study on the effect of temperature and gear pump rotation speed on capsule production and quality

AbstractThis study designed and evaluated an encapsulator unit for producing biocomponent‐containing capsules using sodium alginate. The encapsulator's principle of operation involved maintaining the gel‐forming mixture at a controlled temperature (20–50°C) and utilizing a gear pump to deliver the mixture to a nozzle for capsule formation. Increasing temperature and gear pump rotation speed reduced viscosity, affecting the capsule number, size, and stability. The optimal capsule size and quality were achieved with a sodium alginate concentration of 1%, yielding capsules with rounded shapes, uniform sizes, and soft yet resistant structures. The encapsulation process yielded varying numbers of capsules depending on the rotational speed of the gear pump and the temperature of the gel‐forming mixture. Higher temperatures and rotation speeds generally resulted in increased capsule yields. This research advances encapsulation technology for delivering sensitive biomaterials like probiotics, aiding in process optimization.Practical applicationsThe encapsulator is designed for the production of capsules using structure‐forming compounds and can be used in biotechnology, chemical, food and other industries. The encapsulator allows for the production of capsules with precise size and shell thickness, ensuring high quality.

3D Printing Properties of Heat-Induced Sodium Alginate-Whey Protein Isolate Edible Gel.

The objective of this study was to develop a food 3D printing gel and investigate the effects of whey protein isolate (WPI), sodium alginate (SA), and water-bath heating time on the 3D printing performance of the gel. Initially, the influence of these three factors on the rheological properties of the gel was examined to determine the suitable formulation ranges for 3D printing. Subsequently, the formulation was optimized using response surface methodology, and texture analysis, scanning electron microscopy (SEM), and Fourier-transform infrared (FTIR) spectroscopy were conducted. The rheological results indicated that gels with WPI concentrations of 6-7 g, SA concentrations of 0.8-1.2 g, and water-bath heating times of 10-12 min exhibited lower yield stress and better self-supporting properties. The optimized formulation, determined through response surface methodology, consisted of 1.2 g SA, 6.5 g WPI, and a heating time of 12 min. This optimized formulation demonstrated enhanced extrusion capability and superior printing performance. SEM analysis revealed that the optimized gel possessed good mechanical strength, and FTIR spectroscopy confirmed the successful composite formation of the gel. Overall, the results indicate that the optimized gel formulation can be successfully printed and exhibits excellent 3D printing performance.

One-Step Formation of Pickering Double Emulsion Costabilized by Hydrophobic Silica Nanoparticles and Sodium Alginate.

Pickering double emulsions exhibit higher stability and biocompatibility compared with surfactant-stabilized double emulsions. However, tailored synthesis of particle stabilizers with appropriate wettability is time consuming and complicated and usually limits their large-scale adoption. Using binary stabilizers may be a simple and scalable strategy for Pickering double emulsion formation. Herein, commercially available hydrophobic silica nanoparticles (SNPs) and sodium alginate (SA) as binary stabilizers are used to prepare O/W/O Pickering double emulsions in one-step emulsification. The influence of system composition on double emulsion preparation is identified by optical microscopy, confocal laser scanning microscopy, and interfacial tension and water contact angle analyses. The formation of the O/W/O Pickering double emulsion depends critically on the aqueous phase viscosity and occurrence of emulsion inversion. Both hydrophobic SNPs and SA adsorb at the droplet surface to provide a steric barrier, while SA also reduces interfacial tension and increases aqueous phase viscosity, giving double emulsion long-term stability. Their microstructure and stability are controlled by adjusting the SA concentration, water-oil volume ratio, concentration and wettability of the particle stabilizer, and oil type. As a demonstration, the middle layer of the as-prepared O/W/O Pickering double emulsions can be cross-linked in situ with calcium ions to produce calcium alginate porous microspheres. We believe that our strategy for double emulsion formation holds great potential for practical applications in food, cosmetics, or pharmaceuticals.

Study on the preparation and characteristics of thickened slurry to prevent coal spontaneous combustion

In response to the problems of small diffusion area, short solidification time, and poor flowability of existing fire extinguishing materials in preventing coal spontaneous combustion in goaf areas of western mining areas, this paper studies a thickened slurry with good flowability and fast diffusion ability. The optimal ratio of thickened slurry was determined through orthogonal experiments, and the rheological properties, impregnation characteristics, and wall hanging characteristics of the material were studied. The results showed that when the water to soil ratio was 8:1, and the concentrations of sodium alginate (SA) and apigenin were 0.6 % and 0.5 %, the flowability and suspension performance of the thickened slurry were the best. When the soaking time is 14 days, the best soaking degree of the material can be achieved, with a soaking depth of 0.93 mm. Through wall hanging tests, it was found that the thickened slurry can naturally air dry on the surface of vertically placed coal pillars with a small sliding distance, which has good adhesion and retention characteristics. Further on-site experiments have shown that by injecting thickened slurry, the CO concentration at the return air corner is significantly reduced, indicating that thickened slurry can cover a large area of residual coal in the goaf, prevent contact between coal and O2, and effectively prevent the composite reaction between coal and oxygen.

Formulation, design, and evaluation of valsartan sodium-sustained-release matrix tablets

The main aim of present work was to formulate and evaluate sustain release matrix tablets of Valsartan, an angiotensin II Receptor type 1 antagonist. Sustain release formulation are those which delivers the drug locally or systemically at a predetermined rate for a fixed period of time. The matrix tablet was prepared by direct compression method using by various concentration of chitosan and sodium alginate with combination of various release retardant polymer. The powder mixtures were subjected to various pre-compression parameters such as angle of repose, bulk density, tapped density and Carr’s index shows satisfactory result and the compressed tablets are evaluated for post-compression parameters such as weight variation, thickness, hardness, friability, drug content, in-vitro dissolution and stability studies. In-vitro dissolution studies were carried out for 24 hours using 0.1N HCl for first 2 hours and pH 6.8 phosphate buffers for 24 hours and the result showed that formulations F4 and F7 showed good dissolution profile to control the drug release respectively. Formulation containing higher concentration of chitosan and sodium alginate along with polymers sustainedthedrugreleasefor theperiodof24hours.The compatibilityof thedrug, polymers and other excipients were determined by FT-IR Spectroscopy. Results showed that the drug was compatible with polymers and other excipients. The release data was fitted to various mathematical models such as Zero-order, First-order, Higuchi equation and Korsmeyer- Peppas model to evaluate the kinetics and the drug release. The drug release followed first order and the mechanism was found to be non-Fickian. The stability studies were carried out for 3 months and result indicates that the selected formulations (F4and F7) were stable.

Preparation of Controlled Multicompartmental Gel Microcarriers Based on Aqueous Two-Phase Emulsions for 3D Partitioned Cell Coculture In Vitro.

A facile method was proposed for preparing controllable multicompartment gel microcarriers using an aqueous two-phase emulsion system. By leveraging the density difference between the upper polyethylene glycol solution and the lower dextran-calcium chloride (CaCl2) solution in the collection solution and the high viscosity of the lower solution, controllable fusion of core-shell droplets made by coextrusion devices was achieved at the water/water (w/w) interface to fabricate microcarriers with separated core compartments. By adjusting the sodium alginate concentration, collected solution composition, and number of fused liquid droplets, the pore size, shape, and number of compartments could be controlled. Caco-2 and HepG2 cells were encapsulated in different compartments to establish gut-liver coculture models, exhibiting higher viability and proliferation compared to monoculture models. Notably, significant differences in cytokine expression and functional proteins were observed between the coculture and monoculture models. This method provides new possibilities for preparing complex and functional three-dimensional coculture materials.

W/O/W emulsion-filled sodium alginate hydrogel beads for co-encapsulation of vitamins C and E: Insights into the fabrication, lipolysis, and digestion behavior

In this study, vitamins C and E were simultaneously encapsulated in water-in-oil-in-water (W/O/W) emulsion-filled sodium alginate (SA) hydrogel beads, as well as the effects of SA concentrations (0.5%, 1.0%, 1.5%, and 2.0%) on the structures and lipolysis the of hydrogel beads were investigated. With increasing SA concentration, the beads showed larger sizes, denser structures and better textures. The droplets tightly penetrated the gel network at high SA concentrations. Digestion behavior revealed the disintegrated intramolecular structure at low SA concentrations. The beads with 0.5% SA were fragmented, losing the initial shape during digestion in the intestinal fluid. Additionally, lipid phases were released as W/O/W and O/W emulsion droplets after digestion. However, the high SA concentration-containing beads exhibited a well-preserved morphological structure after digestion, and the release profiles of lipid phase were mainly O/W emulsion droplets. Furthermore, vitamins C and E encapsulated in the beads exhibited high bioaccessibility (vitamin C: 90.20% and vitamin E: 95.19%).

A highly efficient adsorbent adapting to low pH condition for Pb(II) sequestration from aqueous solution − marine diatom: Laboratory and pilot scale tests

Highly efficient, economically feasible and environmentally friendly adsorbents have been a research hotspot for heavy metal sequestration. In the present study, dried marine diatom biomass (mainly composed of Chaetoceros sp.), which possessed high suface area and cumulative pore volume of 5.72 m2/g and 0.028 cm3/g, respectivly, were used to adsorb Pb(II) from aqueous solution. The immoblized marine diatom beads and an adsorption reactor filled with the beads were further investigated for continuous Pb(II) removal. The removal efficiency was evaluated as a function of contact time, initial concentration, pH and co-existing cations. Results indicated that the dried diatom biomass and the immobilized diatom beads effectively adsorbed Pb(II) under pH > 3.0 and pH > 4 0.0 conditions, respectively. The optimal conditions for the immobilized diatom beads preparation were: diatom powder dosage of 5.0 g / 100 mL sodium alginate solution, sodium alginate concentration of 20 g/L, CaCl2 concentration of 2 %, and cross linking time of 1 h. The Pb(II) adsorption on the marine diatom biomass and the immobilized beads were both well described by pseudo-second-order kinetic and Langmuir model. The maximumPb(II) adsorption capacities of the immobilized beads were 919.1 mg/g, which were much higher than that of the most of adsorbents reported elsewhere. Adsorption mechanism analysis demonstrated that Pb(II) was adsorbed onto the diatom biomass mainly through interaction with nitrogen bonds (mainly with pyrrolic nitrogen) and carboxylic groups, and ion exchange with light metals and hydrogen ions in quaternary nitrogen bonds. The diatom beads were used as adsorbents in a continuous Pb(II) treatment reactor, and the Pb(II) removal efficiency of the reactor reached to almost 100 % from the initial concentration of Pb(II) = 50 mg/L.

One‐pot method for instant nanoformulation via self‐assembling of alginate with quaternary ammonium for controlled release of emamectin benzoate

AbstractTo prompt the nanopesticides from laboratory investigation to agricultural application and reduce the use of pesticides and the impacts on the environment, sodium alginate (SA) was self‐assembled with cetyl trimethyl ammonium bromide (CTAB) into stable and uniform nanoparticle under mild conditions. The structure of the nanoparticle was investigated by dynamic light scattering (DLS), ζ‐potential measurement, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) and related to the loading and release properties. It was found that the nanoparticle size increased and ζ‐potential decreased with increasing concentration of SA, leading to higher loading efficiency and sustained release efficacy towards emamectin benzoate (EB). The amount of CTAB had little effects on the size of the nanoparticle, however made a competition to EB and reduced the loading and sustaining efficacy of the nanocarrier. The release of EB from SA‐CTAB nanoparticle was pH‐sensitive and was slower in the medium of higher pH value, due to the increased interaction with the nanocarrier. The entrapment into the nanoparticle greatly increased the insecticidal toxicity of EB due to the interaction between the carriers and cell membrane. The median lethal concentration of typical nanoformulation against Mythimna separata larvae was 30% of that of technical EB, after feeding to the insect for 48 h.

Properties of eco‑friendly orange peel-alginate-glycerol bioplastic films as potential food packaging applications

The development of biofilms is an interesting strategy to reduce the use of conventional packaging materials to preserve and prolong the shelf life of foods. In this study, different concentrations of sodium alginate (SA) (0, 0.5, 1.0, 1.5 and 2.0% w/v) and glycerol (GL) (6.0, 4.0, 2.0, 1.0 and 0% w/v) on the properties of enriched films with orange peel (OP) powder were investigated. Physical, structural, thermal, mechanical, and barrier properties were characterized. The results revealed that incorporation of glycerol to OP-SA film-making solution trend to flexible and opaque films, while unplasticized sample did not form flexible films and were glossy. Moreover, decreasing glycerol and simultaneously increase alginate content led to decrease film thickness, moisture content, water solubility and oxygen transmission rate, whereas water absorption was increased. It was observed in micrographs that films with 1.5% SA and 1.0% GL had better surface attributes, and a further increase in GL and decrease in SA concentration was not found to be as favorable. Furthermore, FTIR spectra exhibited that intermolecular hydrogen bonding occurred between GL and OP-SA. X-ray diffraction showed that the films crystal structure has been changed significantly when the OP-SA and GL were mixed in. The plasticized films showed higher thermal stability as confirmed by TGA and DSC thermogram. The tensile strength of OP-SA-GL films was enhanced from 0.92 to 7.39 MPa, while elongation at break was decreased significantly from 20.76 to 2.67%. Consequently, orange peel-alginate-glycerol films could potentially be implemented in the food packaging.

Effectiveness of sodium alginate-based coating on the preservation of Da xanh pomelo fresh-cut

Pomelo (Citrus maxima (Burm. f.) Merr) fresh cut has a highly sensitive fruit and has a short shelf life after peeling. The fruits represent high potentials for local consumption and international export. The objective of the study was to focus on the effects of different concentrations of sodium alginate (1%, 1.5% and 2%) combined with fixed concentrations of calcium chloride (2%) and ascorbic acid (2.5%) to biologically active compounds and allowable microbial content on the surface pomelo fresh cut slices. The results showed that the presence of coating has improved several important quality parameters of the fruit. Specifically, the combination of AL (2%) + AA (2.5%) + calcium chloride (2%) Ascorbic acid (AA) has effectively maintained the total polyphenol content and the total ascorbic acid of the pomelo slice samples, thus exhibiting higher DPPH scavenging activity, as compared to control samples. Furthermore, the pomelo coated with alginate showed good conditions for consumption with minimal microbial degradation and describe good acceptance after 6 days of storage.

Physicochemical and digestion properties of fish oil and anthocyanins co-encapsulated W/O/W emulsion reinforced with alginate

W/O/W double emulsion systems offer the ability to co-deliver water-soluble and oil-soluble bioactives, making them a promising choice for multifunctional delivery applications. This study focused on the development of a whey protein isolate-based W/O/W emulsion encapsulating fish oil and anthocyanins, with the addition of sodium alginate (SA) into the external aqueous phase. The physicochemical properties, emulsion stability, anthocyanins encapsulation efficiency (EE), and anthocyanins release during simulated gastrointestinal digestion were studied. The results indicated that the physical stability of W/O/W emulsions improved with increasing SA, attributed to the higher viscosity and the protective coating of SA on the WPI-stabilized interface. However, the EE of anthocyanins in SA-reinforced W/O/W emulsions was found to be lower. Excessive SA concentration, such as 1.0 %, was observed to be unfavorable to maintaining the internal W1/O interface integrity. Overall, the double emulsion structure effectively regulated the release of anthocyanins throughout the digestion process. The study concluded that W/O/W emulsions containing 0.75 % SA achieved optimal stability and controlled-release characteristics. These findings offer valuable insights into fabricating double emulsions for multifunctional delivery purposes, showcasing their potential applications in the food and pharmaceutical industries.

Design and Synthesis of P(AAm-co-NaAMPS)-Alginate-Xanthan Hydrogels and the Study of Their Mechanical and Rheological Properties in Artificial Vascular Graft Applications.

Cardiovascular diseases (CVDs) are the number one cause of mortality among non-communicable diseases worldwide. Expanded polytetrafluoroethylene (ePTFE) is a widely used material for making artificial vascular grafts to treat CVDs; however, its application in small-diameter vascular grafts is limited by the issues of thrombosis formation and intimal hyperplasia. This paper presents a novel approach that integrates a hydrogel layer on the lumen of ePTFE vascular grafts through mechanical interlocking to efficiently facilitate endothelialization and alleviate thrombosis and restenosis problems. This study investigated how various gel synthesis variables, including N,N'-Methylenebisacrylamide (MBAA), sodium alginate, and calcium sulfate (CaSO4), influence the mechanical and rheological properties of P(AAm-co-NaAMPS)-alginate-xanthan hydrogels intended for vascular graft applications. The findings obtained can provide valuable guidance for crafting hydrogels suitable for artificial vascular graft fabrication. The increased sodium alginate content leads to increased equilibrium swelling ratios, greater viscosity in hydrogel precursor solutions, and reduced transparency. Adding more CaSO4 decreases the swelling ratio of a hydrogel system, which offsets the increased swelling ratio caused by alginate. Increased MBAA in the hydrogel system enhances both the shear modulus and Young's modulus while reducing the transparency of the hydrogel system and the pore size of freeze-dried samples. Overall, Hydrogel (6A12M) with 2.58 mg/mL CaSO4 was the optimal candidate for ePTFE-hydrogel vascular graft applications due to its smallest pore size, highest shear storage modulus and Young's modulus, smallest swelling ratio, and a desirable precursor solution viscosity that facilitates fabrication.

Thermo‐mechanical properties and optimization of sodium alginate as a polymer backbone substrate for biosensor application

AbstractThis study was aim to optimize the formulation of the sodium alginate biosensor substrate with incorporation of sodium alginate (1%‐6%(w/v)) as a polymer backbone and glycerol (10–50 wt%) as a plasticizer. The effect of their interaction on film's tensile strength (TS) and elongation at break (EB) were analyzed via response surface methodology‐central composite design (RSM‐CCD). The optimum parameter of the films for the maximum TS was at 1.90%(w/v) of sodium alginate and 17 wt% of glycerol and for maximum EB was at 4.89%(w/v) of sodium alginate and 49.65 wt% of glycerol. The optimum parameter of the films had revealed the better TS was at lower sodium alginate content and better EB was at higher glycerol content. In terms of thermal stability, the thermal decomposition temperature (Tonset) of sodium alginate decreased with the incorporation of glycerol.

Construction of a pectin/sodium alginate composite hydrogel delivery system for improving the bioaccessibility of phycocyanin

In this study, different concentrations of sodium alginate were compounded with pectin and phycocyanin to co-prepare composite hydrogel spheres (HP-PC-SA 0.2 %, 0.6 %, 1.0 %, 1.4 %) to evaluate the potential of the composite hydrogel spheres for the application as phycocyanin delivery carriers. The hydrogel spheres' physicochemical properties and bioaccessibility were assessed through scanning electron microscopy, textural analysis, drug-carrying properties evaluation, and in vitro and in vivo controlled release analysis in the gastrointestinal environment. Results indicated that higher sodium alginate concentrations led to smaller pore sizes and denser networks on the surface of hydrogel spheres. The textural properties of hydrogel spheres improved, and their water-holding capacity increased from 93.01 % to 97.97 %. The HP-PC-SA (1.0 %) formulation achieved the highest encapsulation rate and drug loading capacity, at 96.87 % and 6.22 %, respectively. Within the gastrointestinal tract, the composite hydrogel's structure significantly enhanced and protected the phycocyanin's digestibility, achieving a bioaccessibility of up to 88.03 %. In conclusion, our findings offer new insights into improving functionality and the effective use of phycocyanin via pectin-based hydrogel spheres.

Structure Effects on Swelling Properties of Hydrogels Based on Sodium Alginate and Acrylic Polymers.

Hydrogels based on sodium alginate (SA) and partially neutralised poly(acrylic acid) were obtained by radical polymerisation. The hydrogels were cross-linked with N,N'-methylenebisacrylamide (MBA), simultaneously grafting the resulting polymer onto SA. The findings of the FTIR spectroscopy showed that all of the hydrogels were effectively synthesized and sodium alginate was chemically bonded with the poly(sodium acrylate) matrix. DSC analysis of the melting heat and glass transition parameters indicated that the hydrogel structure had changed as a result of the cross-linking process. Sodium alginate and MBA were tested at different concentrations to determine how they affected the hydrogel properties. A very high content of the biopolymer, i.e., sodium alginate, was used in our research, up to 33 wt%. This resulted in durable and stable hydrogels with a very high ability to uptake water, comparable to hydrogels based on synthetic polymers only. The ability to swell is inversely proportional to the quantity of MBA present. By increasing the amount of sodium alginate in the hydrogel, the ability of the hydrogel to absorb water is reduced. However, water uptake remains relatively high at 350 g·g-1, even for the hydrogel with the highest SA content.

Immobilization of Coprinus comatus with magnetic alginate hydrogel microsphere for improving the antioxidant activity of fermentation products

Coprinus comatus is an edible mushroom and its fermented product possesses antioxidant activity. In this study, to further enhance the antioxidant activity and improve the reusability of the strain, calcium alginate hydrogel was used as the carrier for embedding and immobilizing Coprinus comatus. The effects of CaCl2 concentration, sodium alginate concentration, microsphere diameter, and the amount of magnetic particle on the antioxidant activity of fermented products were investigated. The results showed that the magnetic immobilized microsphere prepared by 2.50% CaCl2, 2.00% sodium alginate and 0.50% Fe3O4 had the best fermentation antioxidant activity (EC50 was 0.43 ± 0.01 mg/mL) when the diameter was 5 mm, which increased by 24.56% compared to the initial activity. Besides, the microsphere showed strong reusability, the antioxidant activity was still better than the free strain after being used five times. This study not only enhanced the antioxidant activity of Coprinus comatus fermented product through immobilization, but also provided an effective method for microbial fermentation.