Oil Concentration Research Articles

Study on the ability to inhibit the growth and aflatoxin B1 production from Aspergillus flavus using some essential oils

Vietnam is a country with favorable natural conditions for growing vegetables containing essential oils such as marjoram, dill, lemon basil, perilla, coriander and onions. The objective of this study was to evaluate the inhibition of growth and aflatoxin production in the Aspergillus flavus strain by various essential oils in a culture medium. Samples of marjoram essential oil, dill essential oil, lemon basil essential oil, perilla essential oil, coriander essential oil and onion essential oil were tested for their effects on mycelial growth and aflatoxin production. The findings of this study revealed the antifungal efficacy of all the tested essential oils. The extent of inhibition of fungal growth and aflatoxin production was dependent on the type and concentration of essential oils used. The complete inhibition of A. flavus growth was observed at 1000 ppm concentrations of dill essential oil, lemon basil essential oil, coriander essential oil and onion essential oil. While dill essential oil and lemon basil essential oils showed marked inhibition of aflatoxin B1 produced by A. flavus at all concentrations tested (500, 750 and 1000 ppm).

Influence of oil-water mixing conditions on the friction and wear performance of ship tail-bearing materials

This study addresses the lubrication challenges posed by oil-water mixtures that arise when vessels encounter adverse maritime conditions, including collisions, grounding, and reefing, which can lead to failures in lubrication systems during navigation. The research focuses on three representative ship tail-bearing composites: polymer material(K4), thordon material(SR), and tenmat material(FR). Various volume fractions of oil-water mixtures were prepared, and the rheological properties of these mixtures were examined using a rotational rheometer (MCR102). Additionally, the variation of friction coefficients of the composites about load and linear velocity under different oil-water mixtures was analyzed using a Ring-Block Friction and Wear Testing Machine. Following the experiments, the surface morphology of the composites was assessed, and the wear mechanisms were analyzed using a laser interferometric surface profiler (LI-type), a confocal laser microscope (CLSM), and a scanning electron microscope (SEM). The findings indicate that, under all lubrication conditions, the friction coefficients of the three materials exhibit a gradual decrease with increasing load and linear velocity.Furthermore, the wear of the materials initially increases and then decreases with rising oil content, with higher oil concentrations in the oil-water mixture correlating with reduced wear. The study reveals that the three materials experience significant abrasive and adhesive wear under adverse oil-water mixing conditions. This research offers valuable insights for developing friction substitutes for oil-water mixing bearings in specialized operational environments and guides the design of friction components in such bearings.

Degradation Assessment of In-Service Transformer Oil Based on Electrical and Chemical Properties

In order to ensure the long-term reliability and safety of power transformers, it is important to continuously monitor the characteristics of insulating oil, which not only helps in understanding its behavior over time but also ensures the safety of the equipment. The current study analyzes in-service insulating oil with the aim of relating deterioration and changes in the oil with service aging. Insulating oil samples were collected from three power transformers, with a voltage level of 220 kV and 132 kV, installed at a 220 kV substation. Electrical and chemical characteristics were obtained, and the impact of service aging and the relationships among load variation, oil, and winding temperatures with the characteristics were evaluated. Variations in the dielectric dissipation factor and breakdown voltage with service aging were recorded for all transformers, while the moisture content increased with each service year. Among the concentrations of gases present in the insulating oil, carbon monoxide, oxygen, and nitrogen concentrations increased after each service year. The impact of load variation on the breakdown voltage of the 132 kV transformer oil was more prominent than for the 220 kV transformers. The analysis of gas ratios and moisture content identified the degradation of cellulose insulation in all transformers, which was due to the presence of electrical faults.

Adsorption and flotation in one step: A new method for treating petroleum wastewater

Developing process intensification technologies to treat Petroleum Wastewater (PW) is crucial to maintaining the economic and environmental safety of the process. Therefore, a one-step combination of adsorption and flotation was tested in this study for the first time to remove organic compounds from PW using a biochar-derived composite. High salinity PW samples from Brazilian oil wells were used to test the efficiency of the new method. The tests in synthetic petroleum wastewater found that applying the composite in the combined technique is an efficient alternative for removing organic compounds, presenting better results than using a commercial surfactant. In addition, applying the composite in real-petroleum wastewater showed that it can remove up to 83.89% of oil and grease concentration, even in complex and highly competitive environments. Therefore, its application in batch adsorption and in biosorptive flotation has technical and environmental potential as a viable alternative for treating produced water.

Excess Molar Viscosities and Excess Molar Gibbs Energies of The Mixtures of Tire Pyrolytic Oil + Diesel Fuel at 293.15 K and 303.15 K

Diesel-like fuel mixtures are obtained by blending the pyrolytic oil obtained from the pyrolysis of tires and diesel fuel. The excess thermodynamic properties of blended fuel mixtures give a preliminary idea about the transport, storage and combustion properties of the fuel mixture. In this study, pyrolytic oil and diesel fuel were mixed in different proportions at temperatures of 293.15 K and 303.15 K and their excess molar properties were determined. A positive deviation was observed in the excess molar volume and excess molar Gibbs energy values of the two-component mixture, and a negative deviation was observed in the excess molar viscosity values. Volumetric expansion and flow rate of the mixture were found to be higher at 303.15 K. It has been observed that at low pyrolytic oil concentrations, dispersive and physical forces are dominant between molecules, while at high pyrolytic oil concentrations, π-π interactions are more dominant for the molecules.

Encapsulation of thyme essential oil (Thymus vulgaris) in chia mucilage (Salvia hispanica L.) and its application in fresh pork sausage

This study evaluated the application of thyme essential oil (TEO) encapsulated in chia mucilage in meat sausages as a partial or total substitute for sodium nitrate and nitrite. We assessed three capsules produced with different concentrations of TEO (3.5 %, 7.5 %, and 10 %) in terms of encapsulation efficiency, antioxidant activity, thermal properties, morphology, and antimicrobial activity. Compared to the other concentrations, the 10 % TEO capsules demonstrated higher encapsulation efficiency (98.25 %), antioxidant activity (25.53 %), minimum inhibitory concentrations (0.028 for Escherichia coli and 0.34 for Staphylococcus aureus), thermal stability, and efficacy in inhibiting S. aureus. The oil concentration did not influence capsule yield. We evaluated the physicochemical and microbiological characteristics of different fresh pork sausage formulations: preservatives only (FC), half the concentration of preservatives with the addition of TEO (F1), TEO only (F2), and no preservatives or TEO (F3). The F2 formulation exhibited reduced acidity, peroxide index, and p-anisidine content compared to formulations F1 and F3. Formulations F1 and F2 showed an increased a* parameter (redness) during storage. The F2 formulation exerted microbiological control over coagulase-positive Staphylococcus, E. coli, mesophilic aerobic bacteria, and Salmonella. We concluded that chia mucilage-encapsulated thyme essential oil represents a promising substitute for nitrates and nitrites in meat products.

Обоснование метода нормирования уровня нефтяного загрязнения почв на территории объектов добычи и транспортировки нефти в Арктической зоне

The development prospects of the Arctic zone are associated with oil production and transportation. During operation of oil production and transportation facilities emergency oil spills are possible. The monitoring of the soil cover state requires established standards for permissible oil pollution of soils. The study is aimed at developing a method for local regulation of oil pollution of soils on the territory of oil production and transportation facilities located in the Arctic zone. Soil samples were studied in the local area of the Musyurshorskoye field, including oil production and transportation facilities,. The arbitration IR spectroscopic method was used to determine the concentrations of oil and oil products in the soil. The authors have justified the feasibility of using logistic regression for processing the experimental data array. A method has been developed and parameters for local regulation of oil and oil product content in soils on the territory of oil production and transportation facilities in the Arctic zone have been determined. The presented regulation system allows taking into account the background content of organic matter in soils in the studied area of the territory, classifing soils according to the degree of oil pollution, and identifing signs of an environmental emergency.

Triple Independently Tunable Sensing with Multiple Resonances Based on Metal-insulator-metal Waveguide

In this study, a novel plasmonic refractive index sensor is proposed, featuring a metal–insulator–metal (MIM) waveguide coupled with a composite cavity structure consisting of a double-arc rectangle cavity, a bridge cavity and a three-half-ring configuration cavity. Finite element simulations are used to analyze the transmission characteristics and magnetic field distributions. The results show that six resonance peaks can be produced by three independent resonators. Moreover, there is a good linear relationship between these resonance wavelengths and the structural parameters of the resonators. Then, the refractive index sensing performances of the structure are analyzed by changing the refractive index of the medium within the resonator. The highest sensitivities of the peaks in their resonators are 1732, 2800 and 4568 nm/RIU, respectively. In addition, the proposed structure is tested for the simultaneous detection of the peanut oil concentration of three different blended oils: soybean-peanut, peanut-rapeseed and peanut-sunflower. The ability of the sensor to accurately and simultaneously measure these different oil mixtures highlights its potential for biochemical sensing.

Assessing the Effects of Palm Oil Consumption on Life Expectancy, Metabolic Markers, and Oxidative Stress in Drosophila melanogaster.

Palm oil is the world's second most consumed vegetable oil, sourced from the tropical palm tree Elaeis guineensis. Its consumption has been associated with a higher incidence of cardiovascular disease, largely due to its elevated palmitic acid content, however those studies are contradictory and inconclusive. Wishing to contribute to this issue, the present study aims to investigate the molecular and toxicological effects of this oil and the involvement of oxidative stress, given its role in metabolic dysfunctions using Drosophila melanogaster. This study examines survival rates, and locomotor performance, oxidative status by analysis of lipid peroxidation, ROS formation, thiol levels and antioxidant enzyme activity, and metabolic parameters such as cholesterol and triglycerides, glucose, trehalose and glycogen levels. Exposure to palm oil concentrations of 10% and 30% resulted in a shortened lifespan, reduced locomotor performance, and increased lipid peroxidation, with lower thiol levels and antioxidant enzyme modulation. Cholesterol levels was increased whereas energetic fuels as glucose and glycogen and trehalose were decreased mainly after 10 days of exposure. These findings underscore the detrimental effects of high-fat diets containing palm oil on lifespan, antioxidant defenses, and metabolism in Drosophila melanogaster. This data highlights the potential risk associated with the habitual consumption of palm oil in the daily diet by population, particularly concerning cardiovascular health and metabolic function.

Quantification of mineral oil contamination in corn oil by Fourier transform near-infrared spectroscopy: Comparison of linear and nonlinear multi-calibration methods

Corn oil is a popular edible oil among consumers, renowned for its flavor enhancement and essential nutrient content. Monitoring contaminants in corn oil is critical to ensuring consumer safety. Fourier Transform Near-Infrared (FT-NIR) spectroscopy combined with linear and nonlinear calibration models detected five different concentrations of mineral oil in corn oil. The results demonstrate that Partial Least Squares Regression (PLSR) effectively identifies contaminants such as diesel, white mineral oil, coal oil, and lubricating oil, with determination coefficients (RP2) of 0.99, 0.98, 0.98, and 0.97, respectively, and root mean square error of prediction (RMSEP) values of 1.0240, 1.3435, 1.4318, and 1.6797 mg/kg. The Ridge Regression method yielded optimal performance for detecting motor oil contamination (RP2 = 0.99, RMSEP = 0.64), demonstrating robust reliability. These findings indicate that FT-NIR spectroscopy, when paired with appropriate linear and nonlinear modeling, is an effective tool for ensuring the safety of corn oil.

Experimental investigation on the influence of lubricant oil on CO2 nucleate pool boiling heat transfer characteristics

As a natural working fluid, CO2 is considered the most promising alternative refrigerant to hydrofluorocarbons. In the fields of automotive air conditioning and commercial heat pumps, transcritical CO2 cycles show significant potential for performance enhancement. Although there are many studies on CO2 flow boiling in the open literature, few studies involve CO2 nucleate boiling heat transfer, which is the dominant mechanism of CO2 flow boiling heat transfer process. This study is proposed to conduct experimental investigations of CO2 nucleate boiling heat transfer. The influences of evaporation temperature, heat flux and lubricant oil addition on boiling heat transfer performance and bubble dynamic characteristics are discussed. The results show that in pure CO2 nucleate boiling, heat flux increase leads to higher bubble density and bubble diameter in bulk liquid, which in turn enhances boiling heat transfer. The effect of evaporation temperature increases on bubble diameter is significant. The reduction in bubble diameter weakens the convective heat transfer caused by bubble motion, which leads to less variation in the CO2 nucleate boiling heat transfer coefficient with evaporation temperature. Lubricant oil addition significantly changes the bubble dynamics of CO2 nucleate boiling process, leading to larger bubble density and smaller bubble diameter. Moreover, the oil diffusion at the phase interface notably affects the heat transfer performance, resulting in greater differences in the boiling heat transfer characteristics of the mixtures compared to that of pure CO2. The mixture boiling heat transfer coefficient is collectively influenced by evaporation temperature, heat flux and oil concentration. The experimental results suggest that the heat transfer coefficient of the mixture with an oil concentration of 0.5 % increases by an average of 25 % compared to pure CO2 at an evaporation temperature of 0 °C. At higher evaporation temperatures and high oil concentrations (>1%), oil addition leads to heat transfer deterioration. Findings from this work can provide a better understanding of oil effect on refrigerant boiling heat transfer and a fundamental basis for heat exchanger design of CO2 systems.

The Effects of Phenanthrene on Electrical Activity in Ventricular Cardiomyocytes of Atlantic Cod (<i>Gadus morhua</i>)

The production of oil on the Arctic shelf and its transport along the Northern Sea Route increase risks of pollution of the ecosystems in the Arctic seas with oil and oil products. Today, polyaromatic hydrocarbons are known as the most toxic oil components, and phenanthrene is predominant in terms of its concentration in oil and physiological effects. Phenanthrene affects the electrical activity of fish heart, but its effects are species-specific. At the same time, the effects of phenanthrene on cardiac function in Arctic fishes, including economically important commercial species, are studied not enough. This study examines the effects of phenanthrene on electrical activity and ionic currents in ventricular myocardium of Atlantic cod (Gadus morhua). The major ionic currents in cod myocardium were IKr, IK1, INa and ICa. Phenanthrene (1 μM) did not affect the duration of action potentials (APs) recorded in isolated cod ventricular cardiomyocytes using patch clamp method. Meanwhile, phenanthrene suppressed rapid delayed rectifier current IKr by 61.33 ± 3.94%, decreasing the repolarization reserve of the myocardium. Phenanthrene did not affect nor the level of resting membrane potential, not background inward rectifier current IK1. Also, application of phenanthrene decreased AP upstroke velocity in cod myocytes, which was due to the suppression of fast sodium current INa. Finally, phenanthrene slightly reduced the amplitude of calcium current ICa and accelerated its inactivation, which overall led to the decrease in ICa charge transfer. Thus, the effects of phenanthrene on cod myocardium at cellular level can be described as potentially proarrhythmic, which makes the populations of cod in Arctic seas vulnerable to pollution of the aquatic environment by oil components after oil spills due to technological disasters.

Application of excitation-emission matrix fluorescence spectroscopy and chemometrics for quantitative analysis of emulsified oil concentration

Emulsified oil concentration is an important index for quantitative analysis of sea surface oil spill pollution, and the development of a fast and effective quantitative analysis method for emulsified oil concentration plays a crucial role in the estimation of oil spill volume and post-spill assessment. A quantitative analysis method for emulsified oil concentration based on excitation-emission matrix (EEM) fluorescence spectroscopy and chemometrics was proposed. Firstly, the EEM fluorescence spectra of two emulsified oils were measured using a FLS1000 fluorescence spectrometer. Then, the measured EEM fluorescence spectra were decomposed by parallel factor analysis (PARAFAC), and several key excitation wavelengths were filtered from the loading matrix obtained from the decomposition. Subsequently, the three-band fluorescence index (TBFI) at these excitation wavelengths was calculated and combined with the optimal band selection algorithm, from which the optimal emission band combinations were selected. Finally, the selected optimal emission bands were combined with partial least squares regression (PLSR) to establish a prediction model for emulsified oil concentration. By comparing the prediction results with those based on PARAFAC-PLSR and multivariate curve resolved-alternating least squares (MCR-ALS)-PLSR models, the TBFI-PLSR model showed the best results in the quantitative analysis of emulsified oil concentration. The coefficient of determination, mean square relative error, and ratio of performance to interquartile distance for the gasoline and diesel fuel emulsion validation sets were 0.93, 3.67%, 4.72, and 0.93, 3.72%, 4.60, respectively.

Wound Healing Herbal Formulation from Essential Oil of Lemongrass (Cymbopogon citratus)

Untreated wound may lead to the infection. In some countries, plants are used to cure wound traditionally. Lemongrass (Cymbopogon citratus) has various bioactive compounds, including citronellol, limonene and geraniol that perform some pharmacological activities. In this study, the essential oil of lemongrass was designed into ointment formulation since lemongrass has been reported to have a wound healing effect in mice. The wound healing ointment made from different concentration of the essential oil of Cymbopogon citratus (F1: EOCC 5%, F2: EOCC 10% and F3: EOCC 15%) using a water-removable base and the characteristic as well as the stability (texture, color, smell, homogeneity, and pH) after been stored for 14 days was also evaluated. The result showed that all formulation (F1, F2, and F3) of wound healing formulation revealed good texture, consistency, odor, color, homogeneity and compatible to the skin until 14 days of storage. However, F3 (EOCC 15%) has a strong lemongrass smell compare to the other formulations.

Development and Quality Enhancement of Fried Fish Cake Prototype with Transglutaminase, Trehalose, and Herbal Oil for Room Temperature Distribution.

This study focuses on developing a fried fish cake prototype with improved quality and extended shelf-life, enabling room-temperature distribution through an innovative high-temperature and high-process retort method. Surimi-based products typically necessitate cold storage and a refrigerated distribution system, affecting their physical properties and flavor while escalating costs. By incorporating Transglutaminase (TGase), trehalose, and herbal oils, and optimizing the heating process using the response surface methodology, this research addresses challenges related to changes in physical properties, color, and off-flavors during high-temperature and high-pressure treatment. The addition of 0.37% ACTIVA-K TGase significantly enhanced gel strength by promoting protein cross-linking, while 0.75% trehalose improved color stability by suppressing browning, thus enhancing visual appeal. A 0.1% concentration of bay oil effectively enhanced the flavor profile by masking undesirable odors without compromising the sensory quality. Optimized processing conditions maximized DPPH radical scavenging activity, whiteness, and gel strength, ensuring superior product quality and safety. Nutritional analysis confirmed a balanced composition of moisture, protein, essential amino acids, and minerals, in accordance with Korean national standards for acid values. Microstructural examination revealed a uniform network structure, contributing to excellent texture and sensory evaluations. Shelf-life predictions indicated a storage duration of approximately 19 months, surpassing commercially available products and offering a competitive edge. This novel approach allows surimi-based products to be stored and distributed at room temperature, while also providing the potential for increased profitability.

Effect of oil and particles content on microstructure, rheology, and thermosensitive 3D printability of particles -stabilized high internal phase Pickering emulsions

In this study, soybean protein isolate (SPI) microgel/chitosan (CS) blending system prepared by gel fragmentation were used as particle-based emulsifiers. The presence of chitosan significantly reduced the particle size of the particles and decreased their contact angle (from 129° to 102°), thereby enhancing their wettability. These particles adsorbed onto the oil-water interface by rapidly decreasing the interfacial tension, forming stable O/W-type high internal phase Pickering emulsions. Confocal laser scanning microscopy analysis showed that the droplets were tightly packed together, and some of them were observed to have a polyhedral shape due to the packing, which is the basis for the formation of self-supporting structures. The rheological properties are closely related to the 3D printing results, increasing the oil and particles concentration improves the viscosity, energy storage modulus and gel strength of the system, while the excellent thixotropy facilitates the ability to 3D print these emulsions. At an oil content of 75% and a particles concentration of 3.0%, the accuracy and stability of the printed models were 97.9% and 99.2%, respectively. Introduction of curcumin and NaHCO3 led to a color change from yellow to reddish-brown during heating, enabling thermosensitive 3D printing of color changing HIPPEs. This study may broaden the range of applications of plant-based HIPPEs in 3D and thermosensitive 3D printing, as well as for the development of other innovative food products.

Comparative Efficacy of Ocimum basilicum and Ocimum gratissimum Essential Oils Against Cyclas puncticollis Infesting Sweet Potatoes in Burkina Faso

Aims: The aim of the present study was to compare the insecticidal effect of the essential oils Ocimum basilicum and Ocimum gratisiimum on the sweet potato weevil Cyclas punticollis. Study Design: The aim of this approach was to contribute to the management of the sweet potato weevil in Burkina Faso. Place and Duration of Study: The experiments were carried out at the INERA Entomology laboratory in Bobo-Dioulasso during the cropping season 2022-2023. Methodology: Five essential oil concentrations (25; 50; 75; 100 and 125 μL/L) were used. Toxicity tests were carried out by fumigation in jars by depositing a charge of essential oil on a 6 cm disc of filter paper. Batches of ten C. puncticollis adults were introduced into the jars containing a fresh, healthy tuberous root of the sweet potato and immediately closed. Each test was repeated five times. The number of dead insects in each batch (treated and untreated jars) was counted every 30 min up to 5 h of exposure and for 4 days (24 h, 48 h ,72 h and 96h) per observation. Results: The results showed that the different oils had an insecticidal effect on C. puncticollis and that these oils contained chemical substances responsible for the mortality observed. Indeed, mortality rates of 100% were observed after 2 hours of exposure of the weevils with the 125µL/L concentration of O. gratissimum, followed by concentrations of 100µL/L, 75µL/L, 50µL/L after 24 hours and 48 hours with 25µL/L. With O. basilicum essential oil, 100% mortality was observed after 24 hours of weevil exposure with O. basilicum concentrations of 125µL/L and 100µL/L, followed by 98% mortality with concentrations of 75µL/L, 50µL/L and 25µL/L after 96 hours. Conclusion: These results suggest that the essential oils of Ocimun gratissimun and Ocimun basilicum can be used in the management of C. puncticollis. However, it would be necessary to carry out a concrete application of these oil extracts on large potato stocks in order to confirm or not their efficacy.

Biodegradation of Crude Oil and Aniline by Heavy Metal-Tolerant Strain Rhodococcus sp. DH-2.

Aniline and crude oil are common environmental pollutants that present a significant risk to both the ecological and human health environments. The implementation of efficacious bioremediation strategies is imperative for the elimination of these contaminants. In this study, a bacterial strain designated DH-2 was isolated from soil contaminated with aniline. The strain was identified as belonging to the genus Rhodococcus. The optimal conditions for the growth and aniline degradation by strain DH-2 were determined to be pH 8.0 and 35 °C, respectively. Under these conditions, the degradation rate of aniline at a concentration of 1000 mg/L exceeded 90% within 36 h. Even in the presence of 4% NaCl, the degradation rate remained above 60%. HPLC-MS analysis revealed that the aniline degradation pathway of strain DH-2 follows the catechol pathway. Additionally, strain DH-2 is capable of utilizing crude oil as the sole carbon source, achieving a degradation rate of 91.0% for 2% crude oil concentration within 4 days. In soil modeling experiments, strain DH-2 was observed to degrade aniline and crude oil under triple stress conditions, including 1000 mg/L aniline, 2% crude oil, and 20 mg/L Fe(II) or Pb(II). Complete degradation of aniline and crude oil was achieved after 3 days and 12 days, respectively. The addition of Fe(II) or Pb(II) ions was found to enhance the degradation ability of DH-2. These results demonstrate that strain DH-2 is an extremely effective biodegradable strain, with potential applications in the remediation of environments contaminated with aniline and crude oil, even in the presence of heavy metals.

Essential oils of the Lamiaceae family, their blends and their major compounds in the management of the spider mite, Tetranychus urticae Koch

ABSTRACT This research study focused on determining the lethal concentrations of essential oils extracted from O. basilicum L. Mentha piperita L. Origanum vulgare L. and Thymus vulgaris L. against the two-spotted spider mite (Tetranychus urticae). This notorious agricultural pest wreaks havoc on various plants, causing widespread damage worldwide, as well as their blends and major compounds, using the fumigation method. GC-MS identified the essential oils and major compounds. The essential oils of O. basilicum, M. piperita, O. vulgare, and T. vulgaris had estragole (91.8%), menthol (39.11%), carvacrol (76,8%), and thymol (45,76%) as major compounds, respectively. The most toxic LC50 value was observed with O. basilicum essential oil (8.02 μL.L−1). Blends 7, 8, and 11 showed better results in terms of mite toxicity. The major compounds also displayed promising results. Thus, this study individual presents promising alternatives with potential for managing the two-spotted spider mite.

Inhibitory effect of Eucalyptus globulus essential oil against Botryosphaeria dothidea and Fomitiporia mediterranea fungi causing wood diseases in viticulture

AbstractDieback diseases are caused by Fomitiporia and Botryosphaeria two genus of fungi that colonize wood tissue in grapevine. After the prohibition of sodium arsenite in 2001, no chemical treatments are available to control these diseases. Curettage is a possible substitute solution. The results depend on many factors. The use of essential oils can be an alternative and/or complementary way. This study aims to investigate the essential oil (EO) composition of Eucalyptus globulus (E. globulus) from leaves and flowers and to evaluate its antifungal activity against Botryosphaeria dothidae and Fomitiporia mediterranea fungi. GC–MS results show that 97.79% of oil composition was identified, of which the Eucalyptol is the major compound (76.33%). Other compounds were identified such as α-Pinene (9.81%); Allo-Aromadendrene (3.07%) and Limonene (2.55%). EO had a detrimental effect in vito on both developed Botryosphaeria dothidae and Fomitiporia mediterranea fungi. The effect is dose-dependent and increases with the duration of the treatment. Essential oil concentrations of 0.25% and 0.75% of E. globulus were efficient against Botryosphaeria dothidae and Fomitiporia mediterranea, respectively. This is the first report investigate the purpose of E. globulus EO to control this both phytopathogenic fungi. The results from the antifungal assays reveal that EO of E. globulus has an excellent inhibitory effect against both tested fungi.