Minimizing the concentration of marine microorganisms in ballast water efficiently addressed the potential threat of ecosystem pollution and marine creature invasion. Here we establish a ballast water treatment system without other additional sacrificial agents to in-situ produce H2O2 decomposition further into robust oxygen species •OH for inactivation of marine microorganisms. Typically, the introduction of AgQDs improved the absorbance of visible light and the 7%AgQDs/Bi2O3 catalyst achieved a high photocatalytic sterilization of 87.6 % for bacterial inactivation with a yield of H2O2 that reached 35.62 μM in natural seawater. Besides, the generation of H2O2 is mainly demonstrated via the oxygen reduction reaction pathway, and AgQDs catalyze the generation of •OH from active site occupied an important role rather than H2O2 or •O2–. Meanwhile, we revealed that AgQDs acted as oxygen adsorption centers, activation centers, and photocatalytic reaction centers to facilitate the transportation of charge carriers for enhanced photocatalytic activities, and hot electrons were generated on AgQDs sites with the assistance of visible light illumination to convert molecular oxygen to H2O2 in one step, which offers some experimental reference value for rational treatment of ballast water systems.