Di-(2-ethylhexyl) Phthalate Concentrations Research Articles

A general mechanistic model for predicting the fate and transport of phthalates in indoor environments.

A mechanistic model that considers particle dynamics and their effects on surface emissions and sorptions was developed to predict the fate and transport of phthalates in indoor environments. A controlled case study was conducted in a test house to evaluate the model. The model-predicted evolving concentrations of benzyl butyl phthalate in indoor air and settled dust and on interior surfaces are in good agreement with measurements. Sensitivity analysis was performed to quantify the effects of parameter uncertainties on model predictions. The model was then applied to a typical residential environment to investigate the fate of di-2-ethylhexyl phthalate (DEHP) and the factors that affect its transport. The predicted steady-state DEHP concentrations were 0.14μg/m3 in indoor air and ranged from 80 to 46000μg/g in settled dust on various surfaces, which are generally consistent with the measurements of previous studies in homes in different countries. An increase in the mass concentration of indoor particles may significantly enhance DEHP emission and its concentrations in air and on surfaces, whereas increasing ventilation has only a limited effect in reducing DEHP in indoor air. The influence of cleaning activities on reducing DEHP concentration in indoor air and on interior surfaces was quantified, and the results showed that DEHP exposure can be reduced by frequent and effective cleaning activities and the removal of existing sources, though it may take a relatively long period of time for the levels to drop significantly. Finally, the model was adjusted to identify the relative contributions of gaseous sorption and particulate-bound deposition to the overall uptake of semi-volatile organic compounds (SVOCs) by indoor surfaces as functions of time and the octanol-air partition coefficient (Koa ) of the chemical. Overall, the model clarifies the mechanisms that govern the emission of phthalates and the subsequent interactions among air, suspended particles, settled dust, and interior surfaces. This model can be easily extended to incorporate additional indoor source materials/products, sorption surfaces, particle sources, and room spaces. It can also be modified to predict the fate and transport of other SVOCs, such as phthalate-alternative plasticizers, flame retardants, and biocides, and serves to improve our understanding of human exposure to SVOCs in indoor environments.

Effect of the Plasticizer DEHP in Blood Collection Bags on Human Plasma Fraction Unbound Determination for Alpha-1-Acid Glycoprotein (AAG) Binding Drugs.

Fraction unbound (fu) is a critical drug distribution parameter commonly utilized for modeling efficacious dosage and safety margin predictions. An over-estimation of fu for 13 chemically diverse small molecule drugs primarily bound to alpha-1-acid glycoprotein (AAG) in human plasma was discovered when in vitro results from our screening lab were compared to literature values. Di-(2-ethylhexyl) phthalate (DEHP), a plasticizer known to be used in the manufacture of blood collection bags, was extracted from plasma obtained through three common techniques that allowed contact with DEHP, and drug fu values in plasma from each collection method were estimated using the HTDialysis protein binding methodology. Additionally, fu of test compounds in plasma spiked with varying concentrations of DEHP (0-800μM) was determined, and DEHP extractions were performed from plasma stored in Terumo bags over 7days. Blood stored in Terumo bags, blood collected in Terumo bags, but immediately transferred to conical vials, and vacutainer-collected blood yielded DEHP concentrations of 300-1000μM, 1-10μM, and 0.1-2μM, respectively. This finding corresponded with the fu of tested drugs in DEHP-spiked plasma increasing between 2- and 5-fold. Additionally, DEHP was discovered to leach from the Terumo bag, with concentrations increasing 10-fold over a 7-day test period. In summary, the presence of DEHP in commercially available blood collection bags confounds in vitro fu estimation for drugs that bind primarily to AAG. It is recommended that vacutainer-collected human plasma, which contains negligible DEHP, be used for the most accurate estimation of fu in human plasma.

Characteristics of Phthalic Acid Esters Pollution in Urban Surface Runoff in Shanghai, China

Phthalic acid esters (PAEs) are still widely applied in China, and their pollution characteristics in urban surface runoff are important to receiving water protection. To evaluate the pollution characteristics of PAEs in urban runoff, six priority PAEs in road and roof runoff were monitored in nine storm events from June to September 2017 in Shanghai, China, and related rainwater samples were collected simultaneously. The average ∑6PAEs in urban road and roof runoff were 170.64 μg·L-1 and 40.92 μg·L-1, respectively, much higher than the values reported in Europe and Australia. Di-2-ethylhexyl phthalate (DEHP) was the dominating pollutant in both road and roof runoff. Significance analyses indicated there was no significant difference for low molecular weight(LMW)PAEs concentrations between road and roof runoff, whereas high molecular weight (HMW) PAEs concentrations in road runoff were significantly higher than those in roof runoff and rainwater (P<0.01), which implied that traffic was an important factor contributing to PAEs pollution in urban runoff. The pollutograph of PAEs, total suspended solids (TSS), and chemical oxygen demand (COD) concentrations vs rainfall duration for road runoff showed the same trend, and the first flush effect of PAEs was generally apparent. The influencing factors of PAEs in urban runoff were investigated. EMCs of PAEs in roof runoff were negatively correlated with rainfall intensity and positively correlated with TSS. EMCs of PAEs in road runoff were negatively correlated with rainfall volume and intensity and positively correlated with antecedent dry period, TSS, and COD. PAEs in surface runoff were significantly correlated with particulate matter. According to the criteria of the National Standard of Surface Water Quality of China, DEHP and DBP have limiting values of 8 μg·L-1 and 3 μg·L-1, respectively. The ratios of DEHP concentrations in road and roof runoff to the limiting values are 32 and 7, respectively. DBP concentrations were higher than the limiting value in most rainfall events for road runoff but lower than those for roof runoff. Without reasonable management measures, urban runoff could contaminate receiving water, especially drinking water sources.

Occurrence and spatial distribution of phthalate esters in sediments of the Bohai and Yellow seas

Phthalate esters (PEs) are a class of synthetic chemicals that have been widely used as plasticizers in industrial products and households. The occurrence of PEs in the marine environment has been a concern for many years because of their adverse impacts on marine organisms and human health. In this study, six major PEs, i.e. diethyl phthalate (DEP), di‑isobutyl phthalate (DiBP), di‑n‑butyl phthalate (DnBP), benzylbutyl phthalate (BBP), dicyclohexyl phthalate (DCHP) and di‑(2‑ethylhexyl) phthalate (DEHP), were analyzed in sediment samples collected in the Bohai and Yellow seas. The sum concentrations of the six PEs ranged from 1.4 to 24.6 ng/g and the average was 9.1 ng/g. The highest concentrations of PEs in the sediment samples were those of DEHP with a median concentration of 3.77 ng/g, followed by DiBP (median, 1.60 ng/g), DnBP (0.91 ng/g), DEP (0.32 ng/g), BBP (0.03 ng/g) and DCHP (0.01 ng/g). Generally, concentrations of PEs in the Bohai Sea are higher than those in the Yellow Sea. The varying spatial distributions of the individual PEs can be the result of discharge sources, regional ocean circulation patterns, and mud areas in the Bohai and Yellow seas. Significant positive correlations were found between total organic carbon content and the concentrations of DiBP, DnBP, and DEHP. It is estimated that the inventories of the ∑6PEs were 20.73 tons in the Bohai Sea and 65.87 tons in the Yellow Sea. Both riverine discharge and atmospheric deposition are major input sources for the PE sedimentation, while massive plastic litter and microplastics sinking to the ocean floor can directly release PEs into sediment. This study provides an appropriate data set for the assessment of the risk of PEs to the marine ecosystem.

Risk assessment of endocrine disrupting phthalates and hormonal alterations in children and adolescents

ABSTRACTRisk assessment and hormone evaluation were carried out for di(2-ethylhexyl) phthalate (DEHP) and dibutyl phthalate (DBP), endocrine disrupting chemicals (EDCs), in 302 Korean children (n = 223) and adolescents (n = 79) (< age 19). Urinary and serum concentrations of DEHP, MEHP (mono(2-ethylhexyl) phthalate), DBP, MBP (monobutyl phthalate), and PA (phthalic acid, a common final metabolite of phthalates) were detected in children and adolescents. Daily exposure levels were estimated to be 16.45 ± 36.50 μg/kg b.w./day for DEHP, which is one-third of the tolerable daily intake (TDI) value (50 μg/kg b.w./day), but 14 out of 302 participants had a hazard index (HI = intake/TDI) value >1. The mean daily exposure level of DBP was 1.23 ± 1.45 μg/kg b.w./day, which is one-eighth of the TDI value (10 μg/kg b.w./day), but 1 out of 302 participants had a HI value > 1. Positive correlations were observed between serum DBP or MEHP, and serum estradiol (E2) and/or luteinizing hormone (LH) in prepubescent children. In addition, serum MBP levels were found to be negatively correlated with serum triiodothyronine (T3) or thyroxine (T4) in male participants, and serum DEHP levels with serum thyroid stimulating hormone (TSH) in female adolescents. Low-density lipoprotein (LDL) levels were positively correlated with serum PA levels in children and adolescents. DEHP, DBP or its metabolites may be associated with altered hormone levels in children and adolescents. Data suggest that exposure levels of DEHP and DBP in Korean children need to be reduced to levels below TDI to protect them from EDC-mediated toxicities.Abbreviations: DBP: dibutyl phthalate; DEHP: di(2-ethylhexyl) phthalate; E2: estradiol; EDC: endocrine disrupting chemical; EFSA: European Food Safety Authority; FSH: follicle stimulating hormone; HDL: high density lipoprotein; HI: hazard index; LDL: low density lipoprotein; LH: luteinizing hormone; MEHP: mono(2-ethylhexyl) phthalate; MBP: monobutyl phthalate; PA: phthalic acid; PPAR: peroxisome proliferator-activated receptor gamma; PVC: polyvinyl chloride; T3: triiodothyronine; T4: thyroxine; TDI: tolerable daily intake; TG: triglyceride; TSH: thyroid stimulating hormone; UPLC/MS/MS: Ultra Performance Liquid Chromatography/Tandem Mass Spectrometry; WWF: World Wildlife Fund.

Phthalates and organophosphates in settled dust and HVAC filter dust of U.S. low-income homes: Association with season, building characteristics, and childhood asthma

Phthalates and organophosphates are ubiquitous indoor semi-volatile organic contaminants (SVOCs) that have been widely used as plasticizers and flame retardants in consumer products. Although many studies have assessed their levels in house dust, only a few used dust samples captured by filters of building heating, ventilation, and air conditioning (HVAC) systems. HVAC filters collect particles from large volumes of air over a long period of time (potentially known) and thus provide a spatially and temporally integrated concentration. This study measured concentrations of phthalates and organophosphates in HVAC filter dust and settled floor dust collected from low-income homes in Texas, United States, in both the summer and winter seasons. The most frequently detected compounds were benzyl butyl phthalate (BBzP), di-(2-ethylhexyl) phthalate (DEHP), di-n-octyl phthalate (DnOP), tris (1-chloro-2-propyl) phosphate (TCIPP), triphenyl phosphate (TPHP), and tris (1,3-dichloroisopropyl) phosphate (TDCIPP). The median level of TCIPP in settled dust was 3- to 180-times higher than levels reported in other studies of residential homes. Significantly higher concentrations were observed in HVAC filter dust as compared to settled dust for most of the frequently detected compounds in both seasons, except for several phthalates in the winter. SVOC concentrations in settled dust in winter were generally higher than in summer, while different seasonality patterns were found for HVAC filter dust. Settled dust samples from homes with vinyl flooring contained significantly higher levels of BBzP and DEHP as compared to homes with other types of floor material. The concentration of DEHP and TDCIPP in settled dust also significantly associated with the presence of carpet in homes. Cleaning activities to remove dust from furniture actually increased the levels of certain compounds in HVAC filter dust, while frequent vacuuming of carpet helped to decrease the concentrations of some compounds in settled dust. Additionally, the size and age of a given house also correlated with the levels of some pollutants in dust. A statistically significant association between DEHP concentration in HVAC filter dust in summer and the severity of asthma in children was observed. These results suggest that HVAC filter dust represents a useful sampling medium to monitor indoor SVOC concentrations with high sensitivity; in contrast, when using settled dust, in addition to consideration of seasonal influences, it is critical to know the sampling location because the type and level of SVOCs may be related to local materials used there.

Effects of DEHP on post-embryonic development, nuclear receptor expression, metabolite and ecdysteroid concentrations of the moth Spodoptera littoralis

Di (2-ethylhexyl) phthalate (DEHP) is recognized in vertebrates as an Endocrine Disrupting Chemical (EDC). DEHP can alter steroid hormones production, development, reproduction and behavior in vertebrates. Only few studies investigated DEHP effects on insects. However, some recent studies on aquatic insects showed that DEHP could also act as an EDC by interfering with the signaling pathways of ecdysteroids, the main hormones involved in the control of insect post-embryonic development and physiology. The aim of the study was to investigate (1) the fate of DEHP within a terrestrial insect species by exposing larvae to food containing a wide range of DEHP concentrations and (2) the effects of this chemical on their post-embryonic development and metamorphosis, by using a multi-level approach. DEHP was shown to be present both in larvae and resulting stages, with higher concentrations in chrysalises and adults than in larvae. DEHP concentrations also decreased at the end of the last larval instar, suggesting the metabolic transformation or excretion of this chemical during this time. Only the two highest DEHP doses induced higher insect mortality, whereas low and intermediate concentrations increased larval food consumption without affecting body weight. Metabolic profiles showed that in control insects, the last three days before metamorphosis correspond to a metabolic transition, but with time-dependent changes in treated insects. Interestingly, DEHP treatments also alter both hemolymphatic ecdysteroid titers and expression levels of ecdysteroid response genes. These results confirm that DEHP can alter insect post-embryonic development and metamorphosis, by interfering with ecdysteroid pathways.

Spatial and Temporal Distribution of Di-(2-ethylhexyl) Phthalate in Urban River Sediments.

This study investigated the spatial distribution of di-(2-ethylhexyl) phthalate (DEHP), and its potential biological effects, in the surface sediments that were collected from 10 sites at the Love River during dry and wet seasons. The grain size and organic matter were measured to understand the key factors that affect the distribution of DEHP concentrations in the sediments of Love River. The mean DEHP concentrations in the sediments that were collected during the wet and dry seasons were 28.6 ± 19.5 and 17.8 ± 11.6 mg/kg dry weight, respectively. The highest DEHP concentration was observed in the sediments that were sampled in the vicinity of the estuary. The correlation analysis showed that the grain size and organic matter may play a key role in the DEHP distribution in the sediments during the dry season, whereas the DEHP concentrations in the wet season may be mainly affected by other environmental and hydrological conditions. By a comparison with the sediment quality guidelines, the levels of DEHP in the sediments of Love River were found to have the potential to result in an adverse effect on aquatic benthic organisms. Specifically, during the wet season, wastewater from upstream of Love River is flushed downstream, causing a higher DEHP concentration in the sediments. Future pollution prevention and management objectives should move towards reducing the discharge of upstream wastewater and establishing a complete sewer system to reduce DEHP pollution in the environment.

Phthalic acid esters content in yoghurt with chia flour and bamboo fiber during storage time

Phthalic acid esters are plasticizers, they can migrate freely from plastic to their surroundings. They have negative health effects. European legislation sets specific migration limits for phthalic acid esters. In our study, we deal with two esters of phthalic acid, dibutylphtalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP). It was studied the effect of storage of four types of yoghurt on the concentration of phthalic acid esters. Yoghurts contained 1% chia flour, 5% chia flour, 1% bamboo fiber and 5% bamboo fiber. Yoghurts were stored in plastic cups, which contained both examined phthalic acid esters. Esters of phthalic acid were determined after 1 week of storage then after 2 weeks of storage and in the original raw material. Furthermore, the pH of the yoghurt was determined. The pH values ”‹”‹were correlated with phthalate concentrations: the correlation coefficient for DBP with a pH of -0.0265 and for DEHP with a pH of 0.3075. Mean concentrations of DEHP decreased over time, while DBP decreased for yoghurt with 1% chia flour, while in other cases they increased. The mean DBP concentrations in yoghurt were higher than the average concentrations of DEHP. Comparing the mean sample values ”‹”‹with t-test for dependent samples for yoghurt of the same type, when comparing the DBP or DEHP concentration in week 1 with the DBP or DEHP concentration at week 2, the mean values ”‹”‹were consistent. It can be noted that there was no increase in DEHP concentrations from cups to yoghurts, which was probably due to a lower concentration in cups than the DBP concentration. DBP concentrations increased in 3 of the 4 types of yoghurt. The determined pH in yoghurts did not differ significantly. Apparently a greater effect on the migration of phthalic acid esters will have in our case a different yoghurt consistency than pH. It would be appropriate to examine the effect of food density on the migration of phthalic acid esters. Likewise, it would be appropriate to examine the effect of pH but in the same food with different pH on the migration of phthalic acid esters.

Aggregate Exposure Estimation of Di(2-Ethylhexyl)phthalate in Multiple Cosmetic Products

In order to estimate the realistic exposure of a chemical, aggregate exposure from multiple consumer products should be considered. Typical aggregate exposure assessment was a form of the simply combined amount of exposure by product. Accurate aggregate exposure assessment should be based on individual&amp;#8217;s exposure factors and actual chemical contents in products. The aims of this study were to estimate aggregate exposure of Di(2-ethylhexyl)phthalate (DEHP) in multiple cosmetic products by Korean population group and determine the contribution of each product.We collected the exposure factors of 12 cosmetic types (four skincare, three hair care, three body care, and two cleansing products). Field survey staff visited homes and collected product use information using face-to-face interview. In total, 1,001 subjects over 19 years old completed the questionnaire from June to August 2017. The number of subjects was determined by proportionate quota sampling based on the population composition ratio in sex and age distribution. DEHP concentrations for 111 products from the 12 product categories were analyzed by GC-MS/MS.The exposure factor survey showed that 96.4% of the 1,001 subjects used at least one cosmetic product. The detection frequency of DEHP in the cosmetic products was 61.3% and maximum concentrations were 18.6 µg/g at 34 skincare, 24.0 µg/g at 15 hair care, 122.9 µg/g at 23 body care and 75.2 µg/g at 39 cleansing products. The average aggregate DEHP exposure based on individual&amp;#8217;s exposure factors was 2.97 &amp;#177; 4.00 µg/kg-bw/day in Korea population. The 30&amp;#8217;s women group was the group of the highest aggregate DEHP exposure with 5.21 &amp;#177; 6.17 µg/kg-bw/day which was 6.86 times higher than 60&amp;#8217;s men group. DEHP exposure contribution by products was in order of body cream, facial cleanser and facial cream.These aggregate exposure data would be useful input data for risk assessment and regulation of DEHP in cosmetic products.

Transcriptomic responses of Nile tilapia (Oreochromis niloticus) liver to environmental concentration of di(2-ethylhexyl)phthalate

Di-(2-ethylhexyl) phthalate (DEHP) is currently the most frequently detected phthalic acid esters (PAEs) compounds and can induce diverse toxicities on aquatic organisms. To understand the molecular responses of fish to DEHP, we performed transcriptomic profiles in liver of tilapia (Oreochromis niloticus) which were exposed to environmental concentration of DEHP. A total of 30.10 Mb and 30.16 Mb clean reads were retrieved from the control and DEHP treated libraries, respectively. De-novo assembly of all the clean reads obtained 58,585 unigenes. After comparing the two libraries, 2814 and 1790 genes were identified as significantly increased and depressed, respectively. Gene ontology (GO) classification system and Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis demonstrated that DEHP significantly disturbed the expression level of genes associated with immunity, endocrine and reproductive system, lipid metabolism and so on. Quantitative real-time PCR was performed to validate the results of RNA-sequencing (RNA-seq) analysis. The resulting data provide new insights for exploring the molecular basis of tilapia response to DEHP exposure.

Development of quantum dot aptasensor and its portable analyzer for the detection of di-2-ethylhexyl phthalate

We have developed a quantum dot aptasensor (QD-aptasensor) and its accompanying portable analyzer for the detection of di-2-ethylhexyl phthalate (DEHP). This sensor is based on a newly screened aptamer (60-mer) via SELEX and shows a binding affinity of 213 nmol/L with DEHP. The 60-mer aptamer together with its three shorter truncated aptamers (45, 28, and 22-mer) as well as three different DNA probes (12, 9, and 13-mer) were further investigated to form the best combination for the QD-aptasensor. Using a 22-mer-truncated aptamer and a 12-mer DNA probe combination, the QD-aptasensor demonstrated excellent DEHP sensitivity with an LOQ = 0.5 pg/mL as well as good selectivity in the presence of other phthalate analogs. The binding between the truncated aptamers and DEHP was also characterized. Finally, a QD-aptasensor-based portable analyzer was also developed, and its equivalence to the laboratory protocol was established with a correlation coefficient r = 0.86 for DEHP concentrations ranging from 0.0005 to 100 ng/mL.

UV-B radiation induces DEHP degradation and their combined toxicological effects on Scenedesmus acuminatus

The co-contamination discharge of Phthalate esters (PAEs) by human activities and the increased UV radiation is increasing in aquatic ecosystems. However, little information is available about the combined detrimental effects of UV and PAEs on phytoplankton. In this study, the combined effects of UV-B irradiation and di-(2-ethylhexyl) phthalate (DEHP) on photosynthesis and antioxidant system of Scenedesmus acuminatus, and the DEHP degradation were investigated. Results showed that UV-B radiation decreased the chlorophyll a fluorescence yield, photosynthetic activity (Fv/Fm), pigment content and superoxide dismutase activity. This radiation also increased the reactive oxygen species (ROS) production and soluble protein and malondialdehyde contents. UV-B radiation with 10 mg L−1 DEHP improved the Fv/Fm and alleviated the cell damage of S. acuminatus, and the addition of high DEHP concentration (≥50 mg L−1) aggravated cell damage. The ROS generation also decreased with the increased DEHP concentration. UV-B radiation can effectively promote the DEHP degradation, with the highest degradation rate of 89.9% at an initial DEHP concentration of 10 mg L−1 within 6 h. This result may be attributed to that UV-B irradiance induced DEHP degradation under the regulation of ROS generated by S. acuminatus. Our findings will contribute to the understanding of the combined toxic mechanisms of UV-B and DEHP and in the evaluation of ecological environment risks for primary producers in aquatic ecosystems.

Mechanistic toxicity of DEHP at environmentally relevant concentrations (ERCs) and ecological risk assessment in the Three Gorges Reservoir Area, China

Di-(2-ethylhexyl) phthalate (DEHP) associated in vitro/vivo toxicity at current environmentally relevant concentration (ERC) with attendant ecological risks in the Three Gorges Reservoir Area (TGRA) is still elusive. Responding to this challenge, a novel integrated study based on analytical and biological assays was designed to elucidate the underlying mechanisms for toxicity of DEHP and its ecological risks at ERC. In this study, GC-MS analysis showed that the highest environmental concentration of DEHP in the TGRA surface water was nearly double that of WHO and USEPA standards. Both distribution and ecological risk decreased from the upper to middle and lower reaches of the TGRA. In vitro toxicity was assessed by cell viability and DNA damage assays: DEHP exposure at ERCs (100–800 μg/L) caused significant reduction in cell viability and elevated DNA damage. Further, DEHP exposure above 400 μg/L resulted in enhanced migration behavior of cancer cells. For in vivo toxicity assessment, short term acute exposure (7 d, 400 μg/L) apparently activated the PI3K-AKT-mTOR pathway, and chronic low-level exposure (3 months, 10–33 μg/L) suppressed the hypothalamus pituitary thyroid (HPT) axis pathway in zebrafish. In addition, acute low-level exposure (5 d, 33–400 μg/L) to DEHP increased aryl hydrocarbon receptor (AhR) activity in Tg(cyp1a:gfp) zebrafish in a concentration-dependent manner. In short, DEHP at ERC has extended potential to induce diverse in vitro and in vivo toxicity at concentrations that also cause impairment of biochemical function in aquatic species of the TGRA.

Study on the mechanism of SVOC adsorption onto airborne particles in indoor air

AbstractSemi‐volatile organic compounds (SVOC), such as di‐2‐ethylhexyl phthalate (DEHP), not only cause chemical sensitivity but can also trigger asthma. SVOCs are adsorbed onto airborne particles as well as gas‐phase molecules in indoor air, owing to their low vapor pressure. Therefore, 3 models are suggested to evaluate partitioning between the gaseous and particulate phases using data from previous studies. In this study, the characteristics of each model were extracted, and the mechanisms of DEHP adsorption onto test airborne particles were investigated under controlled temperature, airborne test particle concentration, and air exchange rate conditions in a chamber. The total DEHP concentration increased from 1.6 to 25.6 μg/m3 as the chamber temperature increased from 15 to 35°C. However, the particulate DEHP ratio φ decreased with the increase in temperature. The high airborne particle concentration caused an increase in the particulate DEHP concentration. Moreover, the estimates based on the Junge model corresponded well with the results of these experiments, whereas the Mackay and Pankow models overestimated the values of φ compared to the results of these experiments. These results indicate that particulate surface concentration is related to the DEHP adsorption mechanism onto airborne particles.

Di-(2-ethylhexyl) Phthalate Exposure Modulates Antioxidant Enzyme Activity and Gene Expression in Juvenile and Adult Daphnia magna.

Di-(2-ethylhexyl) phthalate (DEHP) is a plasticizer used in the polyvinyl chloride industry worldwide. DEHP exists in the aquatic environments for decades. However, the toxicological effects of DEHP to aquatic organisms have not been adequately researched. We investigated acute toxicity, oxidative damage, antioxidant enzyme activities, and gene expression patterns of antioxidant enzymes in juvenile and adult Daphnia magna exposed to DEHP. We found that the median lethal concentrations (LC50) of DEHP for juveniles exposed for 24 and 48h were 0.83 and 0.56mgL-1, respectively. The LC50 of DEHP in adults exposed for 24 and 48h were 0.48 and 0.35mgL-1. Daphnia magna that was exposed to DEHP had increased malondialdehyde levels for 24h and lower total antioxidant capacity compared with the control. Activity levels of antioxidant enzymes superoxide dismutase and phase II detoxifying enzyme glutathione S-transferases were significantly higher upon initial exposure for 24h, and enzyme activity was then diminished at high concentrations and prolonged exposure for 48h. Gene expression levels of cat and gst were notably reduced or increased upon DEHP exposure. These findings suggest that DEHP can cause biochemical and physiological effects in juvenile and adult D. magna by inhibiting enzymes, an increase in lipid peroxidation levels and changes both transcription levels of enzymes (cat, gst). On the whole, juveniles and adults both responded similarly to DEHP. Our findings will contribute to the understanding of toxic mechanisms in phthalate esters and the evaluation of environmental risks in aquatic ecosystems.

Variation in accumulation, transport, and distribution of phthalic acid esters (PAEs) in soil columns grown with low- and high-PAE accumulating rice cultivars.

The extensive use of plasticizers containing di-n-butyl phthalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP) results in high residual concentrations in agricultural soils and poses potential risks to human health through the food chain. Here, two rice cultivars with low (Fengyousimiao) and high (Peizataifeng) phthalic acid ester (PAE) accumulation were grown in leaching columns packed with DBP- and DEHP-contaminated soils to investigate their transport, fraction distribution, and accumulation in soil-rice-water system. Significant differences in soil vertical distribution of DBP and DEHP were observed among the two cultivars, sterilization and non-sterilization treatments. Both DBP and DEHP could leach to the bottom layer even though their concentrations in both soil and pore water decreased along with soil depth. DBP and DEHP concentrations in pore water were significantly correlated with those in corresponding soil layer at ripening stage. The available fractions including desorbing and non-desorbing fractions were predominant in the total concentrations of DBP and DEHP of soils. DBP and DEHP storages in coarse soil fractions (i.e., coarse and fine sands) with higher bioavailability displayed higher bioconcentration factors compared to finer soil fractions (i.e., silt and clay), and bioconcentration factors of Peizataifeng were higher than those of Fengyousimiao. The variations in vertical migration and accumulation of DBP and DEHP by the two cultivars implied different adverse effects on the security of groundwater and food.

Prolonged exposure of di(2-ethylhexyl) phthalate induces multigenerational toxic effects in Caenorhabditis elegans

The plasticizer di(2-ethylhexyl) phthalate (DEHP) is an emerging organic contaminant that has represented a risk for organisms present in the environment. However, there is still limited information regarding DEHP-induced multigenerational toxicity and the underlying mechanisms. In this study we investigated the multigenerational toxic effects including locomotive behaviors and reproduction upon prolonged DEHP exposure (from larval L1 to adult) and the underlying mechanisms in the nematode Caenorhabditis elegans. The multigenerational effects were examined over 6 generations (F0–F5) with only parental C. elegans (F0) was exposed to DEHP from larval L1 to adults (72h), and the subsequent offsprings (F1–F5) were grown under DEHP-free conditions. The results showed that prolonged exposure (72h) to various concentrations of DEHP caused dose-dependent locomotive impairments and reproduction defects in C. elegans and that a concentration of 0.2mg/L DEHP was enough to cause such sublethal effects. The results showed that after prolonged exposure to DEHP in the F0 generation, abnormal locomotive behaviors such as reduced body bends and head thrashes were observed from generations F0 to F5. Additionally, prolonged exposure to DEHP (20mg/L) in F0 significantly reduced total brood size in F0, and this parental exposure was sufficient to cause multigenerational reproductive toxicity in the offspring generations (F1–F5) as well. Furthermore, the expressions of reproduction-related genes such as vit-2 and vit-6 were down-regulated by about 20% until F3, and the expression of H3Kme2 demethylase, spr-5, was downregulated in F1 by about 40%. Results from this study demonstrate that prolonged exposure to DEHP only at F0 adversely affected reproduction and locomotive behaviors in C. elegans across generations and might be associated with inadequate vitellogenin production and malfunction of H3Kme2 demethylase. This study implies that parentally prolonged exposure to DEHP caused multigenerational defects in both reproduction and locomotive behaviors raising the potential health and ecological risk.

Experimental study on DEHP affect the neurodevelopment through interfering with placental thyroid hormones transport

Objective: The present study was represented by di-(2-ethylhexyl) phthalate (DEHP), to explore the role of thyroid hormones (THs) disruption in the connection of placenta and neurodevelopmental toxicity. Methods: During fetal mice neural tube closed (pregnancy 9.5 days, E9.5d) to begin synthesis of THs (E15.5 d), all pregnant mice were administered with different concentration of DEHP (0、10、50、200 mg/kg) by gavage once a day(10 mice per group). All pregnant mice were conducted with BrdU administration in E14d by subcutaneous injection. Seven pregnant mice from each group were scarified after anesthesia in E15.5 d, serum and amniotic fluid were collected to determinate the levels of THs(T(3), T(4), FT(3) and FT(4)) by the automatic biochemical analyzer, detecting fetal mice placental protein expression of monocarboxylate transporter 8 (MCT8), organic anion transporting polypeptide 1C1 (OATP1C1) and deiodinaseⅡ&Ⅲ (DIO(2), DIO(3)) by Western blot. Each group of the remaining three pregnant mices were killed after anesthesia in E18d, take the male fetal brain, BrdU immunohistochemistry was used to detect the proliferation and migration of fetal brain cortical neurons. Results: There was no abnormalities in diet, water intake, body weight and general activity of pregnant mice in each treatment group, and there were no difference in the general physiolo. Results There was no abnormalities in diet, water intake, body weight and general activity of pregnant mice in each treatment group, and there were no difference in the general physiological development status of body weight, brain weight, brain body ratio between the mice of each group. There was no statistically significant differences in serum T(3), T(4), FT(3), FT(4) and amniotic fluid FT(4) in pregnant mice of each group (P>0.05), Compared with the control group, the FT(3) levels in the amniotic fluid of the DEHP 50 and 200 mg/kg groups were significantly decreased(P<0.05). Compared with the control group, the placental MCT8 and DIO(2) protein levels of male fetal mice in the DEHP 50 and 200 mg/kg group decreased, and the level of OATP1C1 protein in 200 mg/kg group decreased(P<0.05), and there was no statistically significant difference in DIO(3) protein levels among all groups (P>0.05). Compared with the control group, the number of BrdU positive cells in the cerebral cortex of male mice in DEHP 200 mg/kg group decreased, 56.5% was distributed in VZ-SVZ layer, and the percentage of BrdU positive cells in the IZ layer of 50 mg/kg group increased (P<0.05). Conclusion: DEHP 50, 200 mg/kg may affect the proliferation and migration of neural cells in the developing brain, which may be related to its interference with thyroid hormone by placental transport.

Di (2-Ethylhexyl) Phthalate and Its Role in Developing Cholestasis: An In Vitro Study on Different Liver Cell Types.

Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer used in many polyvinylchloride medical devices and is washed out easily. Thereby critically ill infants can become exposed to DEHP concentrations significantly exceeding the recommended threshold. We suspect DEHP to play an important role in the development of intestinal failure-associated liver disease. The aim of this study was therefore to determine the direct influence of DEHP on different liver cell types. HepG2, human upcyte hepatocytes, primary murine hepatocytes, LX-2, human upcyte hepatic stellate cells, and liver organoids were cultured with DEHP (0.5-500 μmol/L) and parameters including cytotoxicity, cell-cell interactions, and expression of metabolizing enzymes were investigated. DEHP modulated the expression of xenobiotic metabolizing enzymes, reduced the formation of bile canaliculi and cell polarity, and inhibited Cyp-activity in hepatocytes. DEHP had a toxic effect on LX-2 and induced the fibrogenic activation of hepatic stellate cells. The mode of action of DEHP was different in monolayer cultures compared to 3D-liver organoids, which were more sensitive to DEHP. This study suggests that DEHP modulates expression and activity of drug-detoxifying liver enzymes in humans at a clinically relevant concentration. Furthermore, it may contribute to the development of cholestasis and fibrosis. These findings strongly support the opinion, that there is a significant potential for serious adverse effects of DEHP derived from medical devices on human health, especially in very young infants with immature livers.