Concentrations Of Compounds Research Articles

Morphological, Phytochemical and Pharmacological Properties of Carum carvi (Caraway) and Bunium persicum (Black Caraway) Seeds: A Review

Carum carvi and Bunium persicum belongs to Apiaceae family. Both the species are medicinally important and has been used as antimicrobial, antioxidant, anticarcinogenic, antidiabetic, to treat heart problems, spice, condiments and breath freshener or lotions industries etc. Due to morphological similarities, the adulterations are being observed, so it is essential to understand the morphological, phytochemical and pharmacological properties of both the seeds. The plants look similar with approximately similar flower colour. Most prominent difference is in root structure. The roots of C. carvi are fleshy, fusiform tap while that of B. persicum is tuberous. C.carvi seeds are larger than that of B. persicum, with comparatively lighter brown colour. The seeds of C. carvi are sickle shaped while the seeds of the B. persicum are cylindrical in shape. Chemical composition of essential oil is approximately similar with variation in the percentage content of various compounds. Both the seeds are used for food preparation with some variation in their utilization due to difference in their aroma.

Potensi Antimikroba dan Antioksidan Beberapa Ekstrak Daun Benalu (Scurrula ferruginea (Roxb. ex Jack) Danser dari Archidendron sp. terhadap Staphylococcus aureus, Escherichia coli, dan Candida albicans

Scurrula ferruginea (Roxb. ex Jack) Danser is known for its role as an antimicrobial and antioxidant agent due to its content of phenolic compounds, alkaloids, and terpenoids. S. ferruginea has been widely used in traditional medicine for skin infections, diarrhea, hypertension, and digestive tract diseases. This study aims to determine the antimicrobial and antioxidant potential of various treatments of S. ferruginea mistletoe extracts. The experimental method used in this research is the nested pattern experiment. The extract treatments applied include fresh extract, boiled dry extract, boiled fresh extract, brewed dry extract, and brewed fresh extract. Antimicrobial potential was assessed using disc diffusion and dilution methods. Antioxidant activity (IC50) was determined using the DPPH (1,1-Diphenyl-2-Picryl-Hydrazine) method, and Total Phenolic Content (TPC) was also determined. The results indicate that the highest antimicrobial activity against Escherichia coli and Staphylococcus aureus was found in the strong category of fresh extract treatment, whereas there was no significant effect on Candida albicans growth. The fresh extract treatment exhibited the highest antimicrobial activity with a total phenolic content of 20.77321 mgGAE/mL. The Minimum Inhibitory Concentration (MIC) values for the fresh extract against S. aureus and E. coli were 6.25%, and the Minimum Bactericidal Concentration (MBC) values were 12.5% against both bacteria. The antioxidant value of the fresh extract was 101.26 μg/mL, categorized as moderate. Based on the conducted research, it can be concluded that S. ferruginea leaf extract can inhibit the activities of S. aureus and E. coli, while showing no effect on Candida albicans growth.

Examination of internal metabolome and VOCs profile of brewery yeast and their mutants producing beer with improved aroma.

Volatile organic compounds (VOCs) are metabolites pivotal in determining the aroma of various products. A well-known VOC producer of industrial importance is Saccharomyces cerevisiae, partially responsible for flavor of beers and wines. We identified VOCs in beers produced by yeast strains characterized by improved aroma obtained in UV-induced mutagenesis. We observed significant increase in concentration of compounds in strains: 1214uv16 (2-phenylethyl acetate, 2- phenylethanol), 1214uv31 (2-ethyl henxan-1-ol), 1214uv33 (ethyl decanoate, caryophyllene). We observed decrease in production of 2-phenyethyl acetate in strain 1214uv33. Analysis of intracellular metabolites based on 1H NMR revealed that intracellular phenylalanine concentration was not changed in strains producing more phenylalanine related VOCs (1214uv16 and 1214uv33), so regulation of this pathway seems to be more sophisticated than is currently assumed. Metabolome analysis surprisingly showed the presence of 3-hydroxyisobutyrate, a product of valine degradation, which is considered to be absent in S. cerevisiae. Our results show that our knowledge of yeast metabolism including VOC production has gaps regarding synthesis pathways for individual metabolites and regulation mechanisms. Detailed analysis of 1214uv16 and 1214uv33 may enhance our knowledge of the regulatory mechanisms of VOC synthesis in yeast, and analysis of strain 1214uv31 may reveal the pathway of 2-ethyl henxan-1-ol biosynthesis.

Oczyszczanie i waloryzacja ścieków z wytłoczyn z oliwek

Wastewater generated during the production of pomace olive oil is complex and highly variable due to different cultivation and processing characteristics. It has a high toxic organic load, low pH, and high chemical and biological demands. To reduce the concentration of chemical oxygen demand (COD) and total phenolic compounds (TPh) in pomace olive oil wastewater, several treatment processes have been studied. These include: (i) coagulation/flocculation, (ii) electrocoagulation, (iii) peroxy-electrocoagulation, (iv) electrochemical peroxidation, (v) Fenton, (vi) electro-Fenton, (vii) photo-Fenton, and (viii) adsorption. Coagulation/flocculation and electrocoagulation resulted in a maximum COD removal of 16%, while techniques involving the addition of hydrogen peroxide (iii-vii) had an average of 78% TPh removal but only 20% COD removal. Adsorption resulted in a maximum of 29% COD and 75% TPh removal. None of the tested techniques were able to remove more than 50% of COD, indicating the difficulty of removing organic matter in this effluent. However, advanced oxidation techniques were effective in degrading phenolic compounds, although they required relatively high dosages of oxidant.

Drying kinetics, thermodynamic properties and physicochemical characteristics of Rue leaves

Generally, medicinal plants are harvested with high amount of water, so it is essential to subject the product to drying as soon as possible to prevent degradation before application. Most compounds from medicinal plants are sensitive to drying processes, so it is important to adjust the drying conditions. The objective of this study was to describe the drying of Rue (Ruta chalepensis L.) leaves, select the models that best fit each drying condition, determine the activation energy and thermodynamic properties of the leaves, and evaluate their quality after drying. Leaves were harvested with moisture content of 3.55 ± 0.05 kg water kg−1dry matter and subjected to drying at temperatures of 40, 50, 60 and 70 °C. Valcam model showed the best fit to represent the drying kinetics of Rue leaves at temperatures of 40 and 70 °C, and Midilli model proved to be better for the temperatures of 50 and 60 °C. Effective diffusion coefficient increased linearly with the increase in drying air temperature, and the activation energy was 60.58 kJ mol−1. Enthalpy, entropy and Gibbs free energy values ranged from 57.973 to 57.723 kJ mol−1, from − 0.28538 to − 0.28614 kJ mol−1 K−1 and from 147.34 to 155.91 kJ mol−1, respectively, for the temperature range of 40–70 °C. Drying air temperature promoted darkening or tendency to loss of green color; increase in drying air temperature leads to greater discoloration, as well as a higher concentration of total phenolic compounds (about 221.10 mg GAE mL−1 g–1 dm), with a peak at temperature of 60 °C.

Unveiling phenylpropanoid regulation: the role of DzMYB activator and repressor in durian (Durio zibethinus) fruit.

DzMYB2 functions as an MYB activator, while DzMYB3 acts as an MYB repressor. They bind to promoters, interact with DzbHLH1, and influence phenolic contents, revealing their roles in phenylpropanoid regulation in durian pulps. Durian fruit has a high nutritional value attributed to its enriched bioactive compounds, including phenolics, carotenoids, and vitamins. While various transcription factors (TFs) regulate phenylpropanoid biosynthesis, MYB (v-myb avian myeloblastosis viral oncogene homolog) TFs have emerged as pivotal players in regulating key genes within this pathway. This study aimed to identify additional candidate MYB TFs from the transcriptome database of the Monthong cultivar at five developmental/postharvest ripening stages. Candidate transcriptional activators were discerned among MYBs upregulated during the ripe stage based on the positive correlation observed between flavonoid biosynthetic genes and flavonoid contents in ripe durian pulps. Conversely, MYBs downregulated during the ripe stage were considered candidate repressors. This study focused on a candidate MYB activator (DzMYB2) and a candidate MYB repressor (DzMYB3) for functional characterization. LC-MS/MS analysis using Nicotiana benthamiana leaves transiently expressing DzMYB2 revealed increased phenolic compound contents compared with those in leaves expressing green fluorescence protein controls, while those transiently expressing DzMYB3 showed decreased phenolic compound contents. Furthermore, it was demonstrated that DzMYB2 controls phenylpropanoid biosynthesis in durian by regulating the promoters of various biosynthetic genes, including phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), and dihydroflavonol reductase (DFR). Meanwhile, DzMYB3 regulates the promoters of PAL, 4-coumaroyl-CoA ligase (4CL), CHS, and CHI, resulting in the activation and repression of gene expression. Moreover, it was discovered that DzMYB2 and DzMYB3 could bind to another TF, DzbHLH1, in the regulation of flavonoid biosynthesis. These findings enhance our understanding of the pivotal role of MYB proteins in regulating the phenylpropanoid pathway in durian pulps.

Unconventional technologies as a strategy to improve the phenolic and antioxidant potential of zucchini products enriched with blueberry juice

In this study, unconventional technologies were used to enhance the phenolic and antioxidant content of zucchini pieces by enriching them with blueberry juice. Our results indicated that ohmic heating (OH) increased the extraction rates of compounds with antioxidant potential in blueberry juice, mainly at 70 °C for 15 min and 7.8 V cm1. Furthermore, OH applied after vacuum impregnation led to higher retention rates of phenolic compounds and antioxidant capacity in zucchini pieces impregnated with blueberry juice, especially at 70 °C for 30 min and 7.8 V cm‐−1. The impregnation process also promoted the darkening of zucchini pieces (due to anthocyanin retention) and increased soluble solids. After drying by hot air and vacuum microwave, the phenolic content, antioxidant capacity, physicochemical and color characteristics of the impregnated and non-impregnated zucchinis were distinctly altered. However, vacuum microwave drying at 120 and 460 W can be suggested as the most suitable conditions for both zucchini products, as it resulted in high concentrations of individual phenolic compounds (e.g. up to 44.25, 8.15, and 2.56 mg 100 g‐−1 dry weight (DW) for catechin, gallic acid, and cyanidin, respectively), as well as total phenolic content of up to 484.37 mg gallic acid equivalent 100 g1 DW, total flavonoid content of up to 197.69 mg catechin equivalent 100 g‐−1 DW, and total monomeric anthocyanins of up to 9.48 mg cyanidin 3-glucosideo equivalent 100 g‐−1 DW.

Characterization of all life stages of Tenebrio molitor: envisioning innovative applications for this edible insect

The human food security is unavoidably dependent on exploring novel nutritional sources, such as edible insects. However, insects farming needs to improve their rearing practices, such as extracting value from all growing stages, from larvae until adults. In this work, a full characterization of larvae, pupae and adults of Tenebrio molitor was carried out. Higher protein and ash contents were observed in pupae, while higher lipids (mainly polyunsaturated fatty acids, PUFA) were observed in larvae, decreasing until reaching the adult stage. Chitin content was 6-fold higher in adults than larvae and pupae. Also, it was possible to quantify the chitin resorting to computed 3D tomography, achieving similar values as the traditional and chemically based method. Lower mineral content was found in larvae, while high iron, zinc and copper levels were found in beetles. Phenolic compounds content was similar among larvae, pupae and beetles. FTIR analysis was a valuable, easy, and fast method to assess the nutritional composition of T. molitor, detecting vibrations characteristic of protein, lipids and chitin fractions. This study provides a full characterization of all life stages of T. molitor, highlighting the possible valorisation chains that can be adopted to obtain highly nutritive and/or functional compounds.

The Effect of Bay Leaf (Syzygium Polyanthum) Extract Hand Washing Liquid Soap Preparation on The Growth of Escherichia Coli Bacteria in Vitro

Bay leaf (Syzygium polyanthum) is known as a complement to cooking spices, also used by the community for treatment. The content of antibacterial compounds from bay leaves such as alkaloids, flavonoids, saponins and tannins which can inhibit bacterial growth. This study aims to determine the effect of hand washing liquid soap with bay leaf extract on the growth of Escherichia coli bacteria. This study uses a Quasi Experiment research design. The population in this study is located on Jalan Atot Ahmad, West Pontianak District. The sample used was liquid hand washing soap with bay leaf extract at a concentration of 3, 6, and 9%, 9 repetitions with a total of 27 samples, using the disc diffusion method. The results of the antibacterial study showed that the average diameter of the inhibition zone at 3% concentration was 8.27 mm, 6% concentration was 10.55 mm and 9% concentration was 11.16. The results of the Linear Regression test using the Anova test obtained pvalue = 0.000, which is pvalue < α, with α = 0.05, so that it was stated that H1 was accepted so that there was an effect on the preparation of liquid hand washing soap (Syzygium polyanthum) extract on the growth of Escherichia coli bacteria in vitro.

Impact of a minimally processing route for the production of infant formulas on their sensory properties

Infant formulas (IFs), the sole adequate substitute to human milk, undergo several thermal treatments during production that can damage milk proteins and promote the formation of Maillard reaction products, modifying nutritional and sensory properties. The aim of this study was to determine the impact of a minimally processing route based on membrane filtration associated with different levels of heat treatment, on the odor, taste, texture and color attributes of IFs, then to compare with those of commercial milks. Three experimental IFs (produced with membrane filtration associated with low - T-, medium - T+, or high thermal treatments - T+++) were evaluated. Triangular tests conducted with a panel of 50 adults highlighted clear disparities between all the IFs. The same panel applied the Check-All-That-Apply method to evaluate the IFs: the range of variability between T- and T+++ was similar to that between the 2 commercial IFs, and the sensory characteristics of the experimental IFs were not far from the commercial brands for flavor and texture attributes. Analysis performed on the citation frequencies for each descriptor differentiated T-/T+ from T+++, but all the experimental IFs were described with positive sensory characteristics, unlike one commercial IF. Volatile organic compounds (VOCs) content of IFs with low and high thermal treatments were analyzed. Forty VOCs were identified by gas chromatography-mass spectrometry. T- contained a higher quantity of VOCs than T+++, except for benzaldehyde (Maillard reaction product), and aldehydes (oxidation-related products) were the most represented compounds. In conclusion, the processing was associated with sensory differences among IFs, but no marked difference in flavors was found according to CATA and physicochemical analysis. Additionally, no unpleasant sensory descriptors were noted. This shows that the minimally processed route leads to IFs that could fit well within the market from a sensory point of view.

Different formation pathways of nitrogen-containing organic compounds in aerosols and fog water in northern China

Abstract. While aqueous-phase processing is known to contribute to the formation of nitrogen-containing organic compounds (NOCs), the specific pathways involved remain poorly understood. In this study, we aimed to characterize the NOCs present in both pre-fog aerosols and fog water collected at a suburban site in northern China. Fourier-transform ion cyclotron resonance mass spectrometry was utilized to analyze the molecular composition of NOCs in both negative and positive modes of electrospray ionization (ESI− and ESI+). In both pre-fog aerosols and fog water samples, NOCs constituted a significant portion, accounting for over 60 % of all assigned formulas in ESI− and more than 80 % in ESI+. By comparing the molecular composition of NOCs originating from biomass burning, coal combustion, and vehicle emissions, we identified that 72.3 % of NOCs in pre-fog aerosols were attributed to primary anthropogenic sources (pNOCs), while the remaining NOCs were categorized as secondary NOCs formed within the aerosols (saNOCs). Unique NOCs found in fog water were classified as secondary NOCs formed within the fog water (sfNOCs). Through a comprehensive “precursor–product pair” screening involving 39 reaction pathways, we observed that the nitration reaction, the amine pathway, and the intramolecular N-heterocycle pathway of NH3 addition reactions contributed 43.6 %, 22.1 %, and 11.6 % of saNOCs, respectively. In contrast, these pathways contributed 26.8 %, 28.4 %, and 29.7 % of sfNOCs, respectively. This disparity in formation pathways is likely influenced by the diverse precursors, the aqueous acidity, and the gas-phase species partitioning. Correspondingly, saNOCs were found to contain a higher abundance of carbohydrate-like and highly oxygenated compounds with two nitrogen atoms compared to pNOCs. Conversely, sfNOCs exhibited a higher content of lipid-like compounds with fewer oxygen atoms. These results underscore the distinct secondary processes contributing to the diversity of NOCs in aerosols and fog water, which may lead to their different climate effects.

Characterization of the Byproducts of Myrciaria dubia and Psidium guajava and Optimization of the Extraction of Their Bioactive Compounds by Ultrasound-Assisted Extraction and Mechanical Agitation

The food industry generates considerable byproducts that are often discarded and have high contents of usable bioactive compounds. The aim of this study was to characterize the byproducts of camu-camu (Myrciaria dubia) (shell and seed) and guava (Psidium guajava) (shell) production. The extraction and stabilization of the bioactive compounds of camu-camu and guava were also optimized. The variables of ultrasound-assisted extraction (UAE) (shaking time, sonication time and volume–mass ratio) and mechanical shaking-based extraction (MS) (shaking speed, volume–mass ratio and shaking time) were optimized with the surface response method and a Box–Behnken design. The responses studied were total phenolic content (TPC) and antioxidant capacity (AC) evaluated by the degradation of the radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) technique and by the ferric reducing antioxidant powder (FRAP) test. For ultrasound-assisted extraction, the optimal sonication time was 15 min for both the M. dubia and P. guajava shells, and the volume–mass ratios were 50 mL/g for the M. dubia shell and 60 mL/g for the P. guajava shell. However, for M. dubia seeds, there was an agitation time of 3 h, a sonication time of 4.4 min and a volume–mass ratio of 50 mL/g. During extraction by mechanical stirring, the optimal volume–mass ratio for both M. dubia seeds and P. guajava shells was 60 mL/g, while for M. dubia shells, it was 50 mL/g. For the shells and seeds of M. dubia and the shells of P. guajava, the optimal stirring times were 2, 6.4 and 7.7 h, respectively, and the optimal stirring speeds were 172.2, 250 and 256.3 rpm, respectively. Under these optimal conditions, the highest total phenolic content (TPC) results were acquired from the cuma-cuma peel (CCP) extract (26.2 mg gallic acid equivalent (GAE)/g sample) obtained by UAE and from guava peel (GP) extract (27.9 mg GAE/g sample) obtained by MS. The optimized models showed that MS was more efficient than UAE for obtaining bioactive compounds from byproducts of M. dubia and P. guajava. However, UAE required much shorter extraction times than MS. In conclusion, the models obtained for the recovery of bioactive compounds could be applied in large-scale industries to fully exploit the byproducts studied.

Indirect evaluation of watermelon volatile profile: Detection of subtle changes with e-nose systems

The effectiveness of e-nose systems as high-throughput tools for volatile profiling in watermelon was investigated focusing on discerning subtle changes induced by the use of different rootstocks. Partial Least Square Discriminant Analysis (PLS-DA) models, both GC-MS and e-nose data, demonstrated moderate performance in classification due to nuanced differences among groups (the same F1 hybrid was used as scion). However, PLS-DA biplots revealed a clear correlation between GC-MS and e-nose data. This methodology enabled the e-nose system to identify the effects of specific root-scion combinations compared to non-grafted controls and detect combinations with more variable volatile profiles. Remarkably, the e-nose system identified samples with anomalous volatile profiles, mirroring the capabilities of GC-MS data. Additionally, PLS models were developed to provide reasonably accurate predictions of key compound contents like geranylacetone, (Z)-6-nonen-1-ol, or (Z)-6-nonenal, crucial for watermelon flavor and taste perception. Overall, this study highlights the potential of e-nose systems in discerning nuanced variations in watermelon volatile profiles affecting aroma. Incorporating volatile profile evaluation capabilities using such systems will significantly optimize quality control processes and plant breeding programs.

Perfluoroalkyl compounds in groundwater alter the spatial pattern of health risk in an arsenic‑cadmium contaminated region

Integrated health risk assessment strategies for emerging organic pollutants and heavy metals that coexist in water/soil media are lacking. Contents of perfluoroalkyl compounds and potentially toxic elements in multiple media were determined by investigating a county where a landfill and a tungsten mine coexist. The spatial characteristics and sources of contaminants were predicted by Geostatistics-based and multivariate statistical analysis, and their comprehensive health risks were assessed. The average contents of perfluorooctane acid, perfluorooctanesulfonic acid, arsenic, and cadmium in groundwater were 3.21, 0.77, 1.69, and 0.14 μg L−1, respectively; the maximum content of cadmium in soils and rice highly reached 2.12 and 1.52 mg kg−1, respectively. In soils, the contribution of mine lag to cadmium was 99 %, and fertilizer and pesticide to arsenic was 59.4 %. While in groundwater, arsenic, cadmium and perfluoroalkyl compounds near the landfill mainly came from leachate leakage. Significant correlations were found between arsenic in groundwater and arsenic and cadmium in soils, as well as perfluoroalkyl compounds in groundwater and pH and sulfate. Based on these correlations, the geographically optimal similarity model predicted high-level arsenic in groundwater near the tungsten mine and cadmium/perfluoroalkyl compounds around the landfill. The combination of analytic network process, entropy weighting method and game theory-based trade-off method with risk assessment model can assess the comprehensive risks of multiple pollutants. Using this approach, a high health-risk zone located around the landfill, which was mainly attributed to the presence of arsenic, cadmium and perfluorooctanesulfonic acid, was found. Overall, perfluoroalkyl compounds in groundwater altered the spatial pattern of health risks in an arsenic‑cadmium contaminated area.

Commercial wine yeast nitrogen requirement influences the production of secondary metabolites (aroma, hydroxytyrosol, melatonin and other bioactives) during alcoholic fermentation

During alcoholic fermentation, Saccharomyces cerevisiae synthesizes different compounds, which are crucial for product quality: volatile compounds with sensory impact, and bioactive compounds such as melatonin (MEL) and hydroxytyrosol (HT), linked to health benefits. As many of these compounds are related with yeast's nitrogen metabolism, their production have been studied in four different commercial strains with different nitrogen requirement (Red Fruit, Uvaferm VRB, Lalvin Rhone 2323 and Lalvin QA23) being, Uvaferm UVR the higher nitrogen demander strain. All strains produced the secondary metabolites, notably Uvaferm UVR produced the highest HT concentration, despite its low growth. Uvaferm UVR emerged also as a significant producer of MEL, indicating a potential role in fermentation related stress. Moreover, Uvaferm UVR shows the highest total concentrations of volatile compounds. Multivariate analysis revealed distinct clustering based on nitrogen requirements of the strains, highlighting the strain-dependent metabolic responses.

Irrigation Practice in Cultivation of Stevia rebaudiana Bertoni in Cooler Climates—Case Study in Poland

Stevia rebaudiana Bertoni originated in South America, but has gained popularity due to the concentration of sweet steviol glycosides present in its leaves. Stevia is also currently grown on other continents in many countries characterised with warm climate. This research aimed to determine whether different irrigation practices might have a significant influence on the yield and composition of the leaves of stevia plants grown in Poland, in cooler climate than native regions for stevia plants. Stevia plants were grown with four different irrigation regimes: irrigation twice a week, irrigation once a week, irrigation only during times of the drought and not irrigated at all. It was determined that different irrigation regimes have substantial effects on the yield, biometric characteristics and chemical composition of stevia leaves, but none of the irrigation regimes were clearly supreme to the others. Yield of stevia plants that were irrigated once a week or only during the drought times was the highest (2.42–2.58 kg per square meter). Stevia plants that were not irrigated were characterised with the lowest height and lateral width (47.61 and 25.35 cm). Leaves of un-irrigated stevia contained the greatest dry mass content (30.47%) and highest concentration of phenolic compounds (337.88 mg per 100 g of fresh stevia leaves). Stevia plants that were irrigated once or twice a week were characterised with a lower concentration of chlorophylls or carotenoids at the time of the harvest (1.20–1.24 and 2.54–2.58 mg per gram of fresh stevia leaves), but stevia plants that were not irrigated contained a greater amount of chlorophyll and carotenoids (1.58 and 3.17 mg per gram of fresh stevia leaves).

Cytotoxicity Screening of Endophytic Fungi from Phaleria macrocarpa (Scheff) Boerl) Collected in Yogyakarta District

Cancer is a disease that is a global problem with a high prevalence and mortality rate. Indonesia has biodiversity with enormous potential as a source of medicinal ingredients. Phaleria macrocarpa (Scheff) Boerl) It is known to contain active metabolites, potentially an anticancer. Some endophytes are reported to be able to produce secondary metabolites similar to their parent plants. This study aimed to obtain endophytic fungi from P. macrocarpa capable of producing compounds with anticancer potential. Endophytes producing anticancer compounds were explored by screening endophytic fungi producing secondary metabolites from parts of the plant P. macrocarpa collected in the Yogyakarta area. The anticancer bioassay (MTT assay) was performed in vitro using cancer cells T47D, MCF7, HeLa, and normal Vero cells. Potential extracts were traced for their mechanism of cell cycle modulation and apoptosis using Flowcytometry. First, fungal identification was carried out on fungi producing potential cytotoxic extracts. Then the compound content was analyzed using LC-HRMS. The results from the six extracts obtained from fruit, leaf, and stem parts showed that the endophytic extract from the leaves (code PC-L2) had the best cytotoxicity compared to other endophytes and showed the best result on MCF-7 cells with an IC50 value of 110.66 µg/mL. The producing PC-L2 extract fungi are closely associated with Clonostachys wenpingii strain DUCC5606. This extract induced apoptosis but not through the mechanism of cell cycle modulation.

The content and distribution pattern of chemicals and bioactive compounds of triticale grains

SummaryThere is accumulating evidence that triticale‐based foods have potential health benefits. Herein, we milled triticale grains into five components (break flour, reduction flour, coarse bran, fine bran and whole flour) and analysed their nutritional profiles. Our findings revealed that triticale flour has higher content of minerals (2.04 g 100 g−1), insoluble dietary fibre (14.58 g 100 g−1), polyunsaturated fatty acids (1055.3 μg g−1) and vitamin B9 (0.031 mg 100 g−1) and lower levels of lipids and total essential amino acids than wheat flour. All milling fractions showed higher amounts of unsaturated than saturated fatty acids. Coarse bran and fine bran had the highest mineral and vitamin B content. Break flour has significant amounts of total and essential amino acids, whereas reduction flour has significant amounts of starch and amylose. Break and reduction flour have high mineral bioaccessibility. Thus, milling fractions have unique nutritional properties, which is important for the comprehensive utilisation of triticale.

Chlorophyll a fluorescence and phytochemical response in blackberry species (Rubus sp) cultivated in the high tropics

BACKGROUND: The evaluation of chlorophyll fluorescence activity is a useful tool for rapid and effective assessments of the effect of abiotic factors on the physiology of different crops. In Rubus, this type of measurements describes tolerance to high temperatures, sensitivity to heat and morphological alterations in flowering and chlorophyll content, as a result of plant stress. OBJECTIVE: The present research estimated chlorophyll fluorescence and the presence of some phenolic compounds in the leaves of three cultivars of blackberry (Rubus sp.) established in the central region of Colombia, under conditions of open air between 2400 and 2800 masl. MATERIAL AND METHODS: This was done through the measurement of chlorophyll a (Junior-PAM II modulated fluorometer), the quantification of phenolic compounds (Folin Ciocalteu), the determination of antioxidant capacity (DPPH and ABTS) and the study of phenolic profiles (UHPLC) in leaves of three blackberry cultivars. RESULTS: Cultivars R. alutaceus (0.66), R. glaucus (0.73) and R. alpinus (0.76), presented values lower than the optimum (0.83), for the parameter Fv/Fm. In addition, the direct relationships between the parameters that define the behavior of PSII and the accumulation of different polyphenols were described. Reference was also made to the content of phenolic compounds (ranging from 125 to 150 mg GA g–1 dw), which did not show significant statistical differences. The heat map and principal component analysis show the close relationship between the parameters that characterize the functioning of PSII and the accumulation of phenolic compounds, as a mechanism of adaptation to the adverse conditions of the blackberry cultivars studied. CONCLUSIONS: The results reveal that the leaves of the Rubus studied could be a source of functional ingredients for use in the cosmetic, nutraceutical and food industries and epigallocatechin was attributed the greatest responsibility for the antioxidant capacity.

Research on the pharmacognostic characteristics, physicochemical properties and in vitro antioxidant potency of Rosa laxa Retz. flos

AbstractRosa laxa Retz. is an unexplored Rosaceae plant in Xinjiang, China, and its flower is traditionally used in Kazak to treat the common cold, fever, and epileptic seizures and lessen the effects of aging. In the present study, the pharmacognostic profiles, physicochemical properties, phytochemical characteristics, and in vitro antioxidant potency of Rosa laxa Retz. flos (RLF) were presented. In the pharmacognostic evaluation of RLF, organoleptic characteristics, internal structures, and powder information were observed, and physicochemical parameters, including moisture content, ash, pH value, swelling degree, and extractives were examined. The quantitative analysis of the chemical composition of four different polar extracts of RLF showed that the aqueous part had the highest total triterpene acid, flavonoid, and polyphenol content (4.50 ± 0.04 mg/g, 50.56 ± 0.03 mg/g, and 60.20 ± 0.09 mg/g, respectively). A high‐performance liquid chromatography (HPLC)‐diode array detector (DAD) method was established and the contents of gallic acid, ellagic acid, astragalin, and tiliroside in RLF were determined simultaneously. In the set concentration range, the linear relationship among the four components was good (r > 0.999), the average recoveries were 97.36%–100.54%. The contents of gallic acid, ellagic acid, astragalin, and tiliroside in RLF samples were (9.46 ± 2.31) mg/g, (10.60 ±0.75) mg/g, (1.13 ± 2.50) mg/g, and (1.11 ± 2.65) mg/g, respectively. The types of its secondary metabolites were determined by fluorescence, color reaction by chemical solvent method, and ultraviolet–visible (UV–Vis) spectroscopy. The functional groups of its secondary metabolites were determined by Fourier transform infrared (FTIR) spectroscopy. Results showed that RLF contains a variety of secondary metabolic products, including flavonoids, phenolic acid, glycoside, and organic acid. TLC identification showed it contains ursolic acid, β‐sitosterol, tiliroside, astragalin, isoquercitrin, kaempferol‐3‐O‐rutinoside, gallic acid, and ellagic acid. The in vitro antioxidant activity of different polar parts of RLF was investigated by DPPH, ABTS, and reduction performance experiments. The aqueous extract had the strongest antioxidant capacity, consistent with the high content of triterpene acids, flavonoids, and polyphenolic compounds. These findings will provide critical information for the study of quality standards and medicinal value of RLF and its extracts, justify its usage in traditional medicinal systems, and encourage the use of this plant in disease prevention and treatment. Its phytochemical composition and pharmacological studies need to be explored in future.Research Highlights Optical microscope and scanning electron microscope (SEM) were used to observe the morphology, and microstructure of Rosa laxa Retz. flos (RLF). The physicochemical properties, fluorescence and phytochemical composition of four different polar extracts of RLF were analyzed by UV–Vis and FTIR. Determination of total triterpenic acid, total flavonoids, and total polyphenols in four different polar extracts of RLF by UV spectrophotometry. A high‐performance liquid chromatography (HPLC)‐diode array detector (DAD) method was established and the contents of gallic acid, ellagic acid, astragalin, and tiliroside in RLF were determined simultaneously. TLC confirmed that RLF contains ursolic acid, β‐sitosterol, tiliroside, astragalin, isoquercitrin, kaempferol 3‐rutinoside, gallic acid, and ellagic acid. The in vitro antioxidant activity of RLF was studied by DPPH, ABTS, and reducing ability experiments.