Concentration Of Cinnamic Acid Research Articles

Inter-tree Variation in Chemical Components of North Sumatra Benzoin Gum (Styrax sp.)

Benzoin gum, which is known as Sumatra benzoin (Styrax sp), is widely used as an ingredient in the incense. Although these resins are widely used as flavours and fragrances, no studies have been made on inter-tree variation of its chemical composition. Therefore, benzoin gum samples were tapped from the 30 individual trees (age 10 years, 9~35 cm in diameter) grown in the community forest of Polung, Humbang Hasundutan Regency, North Sumatra. The gum chemical components were analyzed by GC-MS and identified by comparing the fragmentation pattern with the standard components and literature studies. It was found that the major compounds detected by GC-MS were cinnamic acid (51.48%), cinnamyl cinnamate (62.56%), benzoic acid (1.94%), chavicol (5.18%), benzyl cinnamic acid (7.8%), atropic acid (9.84%), and vanillin (1.47%). Two main constituents that were always detected from 30 benzoin trees were chavicol and cinnamic acid, followed by cinnamyl cinnamate (28 trees) and benzoic acid (14 trees). By cluster analysis, 30 samples of benzoin gum can be classified into clusters I - III based on the average chemical components. Cluster I consisted of 7 individual trees with a higher percentage of benzoic acid and chavicol but lower concentration of cinnamic acid compared to other clusters. Cluster II consisted of 18 individual trees with a high percentage of cinnamyl cinnamate whereas clusters III consisted of 5 individual trees characterized with a high percentage of cinnamic acid. By Pearson correlation, it was observed that no significant correlation between the values of diameter and the amount of chemical components of benzoin gum.

Effect of Padina gymnospora biowaste inclusion on in vitro methane production, feed fermentation, and microbial diversity.

In vitro studies were undertaken aiming to study the methane (CH4) mitigation potential of biowaste (BW) of Padina gymnospora at the graded inclusion of 0% (C), 2% (A2), 5% (A5), and 10% (A10) of the diet composed of straw and concentrate in 40:60 ratio. The chemical composition analysis revealed that the BW contained higher crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), and ether extract (EE) than the PF (fresh seaweed, P. gymnospora). The concentration of cinnamic acid, sinapic acid, kaempferol, fisetin p-coumaric acid, ellagic acid, and luteolin in BW was 1.5-6-folds less than the PF. Inclusion of BW decreased (P < 0.0001) CH4 production by 34%, 38%, and 45% in A2, A5, and A10 treatments, respectively. A decrease (P < 0.0001) of 7.5%-8% in dry matter (DM) and organic matter (OM) digestibility was also recorded with the BW supplementation. The BW inclusion also decreased the numbers of total (P = 0.007), Entodinomorphs (P = 0.011), and Holotrichs (P = 0.004) protozoa. Metagenome data revealed the dominance of Bacteroidetes, Proteobacteria, Firmicutes, Actinobacteria, and Fibrobacter microbial phyla. At the phylum level, Euryarchaeota dominated the archaeal community, whereas Methanobrevibacter was most abundant at the genus level. It can be concluded that the inclusion of BW in straw and concentrate based diet by affecting rumen fermentation, protozoal numbers, and compositional shift in the archaeal community significantly decreased CH4 production. Utilization of biowaste of P. gymnospora as a CH4 mitigating agent will ensure its efficient utilization rather than dumping, which shall cause environmental pollution and health hazards.

PHENOLIC COMPOUND AND ANTIOXIDANT POTENTIAL OF Hebeloma sinapizans MUSHROOM

Mushrooms have been used for both culinary and medicinal reasons for a very long time. Mushrooms are useful as both a food and a medication due to their high nutritional, protein, vitamin, and phenolic content. The current research aims to determine the antioxidant activity and phenolic content of Hebeloma sinapizans. Multiple epidemiological research looked at the possible link between polyphenol consumption and cardiovascular illness. Antioxidant, antiplatelet, antiinflammatory, antilipidemic, and antihypertensive are the categories used to classify the actions of phenolic substances. HPLC was used to isolate the phenolic compounds (HPLC). Measurements of oxidative stress, total oxidant levels, and total antioxidant levels were all calculated with the use of Rel Assay kits. The mushroom's TAS, TOS, and OSI values were measured, and its gallic acid, chlorogenic acid, and cinnamic acid concentrations were identified. Due to its high levels of phenolic compounds, H. synapizans is thought to be a source of phenolic compounds. High TAS and TOS values and low OSI values indicate that the mushroom has antioxidant potential. As a result, it has been seen that H. synapizans can be a good source of antioxidants.

Changes in Profiles of Classes and of Individual Polyphenols in Leaves of Spiraea chamaedryfolia and Spiraea media along an Altitudinal Gradient.

Plants in high-altitude habitats are exposed to severe environmental stressors, including extreme temperatures and irradiation, which can have wide-ranging effects on changes of secondary-metabolite profiles in higher plants. Altitude-related variation of levels of polyphenols in organs of medicinal and food plant species has not yet been investigated sufficiently. This study was focused on variation in quantitative profiles of classes and of individual biologically active phenolic compounds in leaf extracts of resource species Spiraea chamaedryfolia and Spiraea media from the family Rosaceae in coenopopulations of the Altai Mountains, along an altitudinal gradient. High-performance liquid chromatography revealed 22 polyphenolic compounds in the extracts of S. media leaves, with the main polyphenolic compounds being flavonols. Sixteen compounds were found in S. chamaedryfolia leaf extracts, and the major ones were flavonols and a flavanone. Opposite responses to changes in the altitude-associated growth conditions were documented for levels of some individual polyphenolic compounds. With an increase in altitude, concentrations of chlorogenic acid and of flavanone in the extracts of S. chamaedryfolia leaves significantly increased, while concentrations of cinnamic acid, astragalin, and kaempferol diminished. A statistically significant positive correlation between the altitude of plant habitats and total levels of polyphenols and phenolcarboxylic acids was detected. In leaf extracts from S. media, an altitude increase was significantly positively correlated with astragalin, avicularin, and cinnamic acid levels and negatively correlated with hyperoside concentration.

Silencing the novel gene CsARR-9 increases photosynthetic efficiency and alleviates autotoxicity in cucumber

Autotoxicity is a principal cause of growth inhibition in monocultures. Autotoxicity in monocropped cucumber limits plant growth, which causes an obvious reduction in yield and quality in cucurbit crops. Cinnamic acid is a major autotoxin that has been used to mimic autotoxicity in cucumber. The long hairpin RNA (lhRNA) library of Arabidopsis was screened in cinnamic acid (CA) mimicked autotoxicity to obtain autotoxicity-resistant mutant lines. We found that a new gene, At5g16610, whose function is unknown, was silenced in the resistant mutant line HCA350–597, and the homologous gene of Arabidopsis At5g16610 (NM_203062.2), whose function is also unknown, was cloned in cucumber and named CsARR-9 (Cucumis sativus Autotoxicity Resistance Related). Here, we silenced the CsARR-9 gene using an established tobacco rattle virus (TRV) mediated virus-induced gene silencing (VIGS) system, and the CsARR-9 gene was also silenced by RNA interference in cucumber. We found that this silencing resulted in decreased CsARR-9 expression in both leaves and roots enhanced tolerance to autotoxicity mimicked by CA. Resistance to CA-mimicked autotoxicity was achieved by reductions in the CA concentrations in roots, stems and leaves, which maintained the structural integrity of cells and tissue within an organ system, promoted cell vigor, enhanced thylakoid stacking and increased the storage of photosynthetic products in chloroplasts, and these effects promote the growth of CsARR-9-silenced cucumber plants. These findings provide a valuable genetic resource for research regarding continuous cropping obstacles, especially in monocultures, and insights on the related molecular mechanism, and have important practical significance for prolonging the planting years and the efficient use of facility agriculture.

Synthesis of butyl cinnamate using enzymatic membrane reactor in a free solvent system

Cinnamic acid is a phenolic compound that has the potential to act as a natural antioxidant. Cinnamic acid esterification can be performed by adding alcohol as an alkyl group donor. This esterification is carried out to increase the antioxidant capacity of cinnamic acid. Esterification of cinnamic acid with butanol as the alkyl group donor was the best condition to modify cinnamic acid. In this study, the synthesis of butyl cinnamate using a enzymatic membrane reactor (EMR) is carried out continuously with Lipozyme TL IM as the catalyst. This study aimed to determine the optimal conditions for the synthesis of butyl cinnamate using various concentrations of cinnamic acid, biocatalyst, residence time, and the presence of molecular sieves. The synthesis of butyl cinnamate at 40°C was optimum when molecular sieves were present with 0.01 M cinnamic acid concentration, 1% (w/v) biocatalyst, and 12 h of residence time.

Magnetic nanofluid based on hydrophobic deep eutectic solvent for efficient and rapid enrichment and subsequent determination of cinnamic acid in juice samples: Vortex-assisted liquid-phase microextraction

A quick, environmentally friendly and easy approach for the determination of cinnamic acid in juice samples based on the creation and usage of a novel magnetic nanofluid (mixture of hydrophobic deep eutectic solvent and magnetic nanoparticles) has been reported. Response surface methodology was applied to justify the contribution of the efficient factors including pH, nanofluid volume, ionic strength and vortex time. Cinnamic acid concentrations were monitored and quantified based on their HPLC peak representing linear correlations under the best operational circumstances showing linearity between 3 and 550 ng mL−1. The LOD, LOQ, and enrichment factor for cinnamic acid were 0.8 ng mL−1, 2.7 ng mL−1 and 57.2, respectively. The proposed method was used for enrichment and subsequent determination of cinnamic acid from juice samples which suggests a potential alternative approach for cinnamic acid analysis in complicated food samples.

Bazı Dicranum Hedw. Türlerinin Biyokimyasal Özellikleri

In this study, the fatty acid compositions, lipid-soluble vitamins, sterols, flavonoids, and phenolic acid levels, as well as the DPPH radical scavenging activity of five different Dicranum species (D. majus, D. fuscencens, D. polysetum, D. scoparium and D. tauricum) were examined. While lipid-soluble vitamins, sterols, flavonoids, and phenolic acids were evaluated by HPLC, fatty acid compositions were assessed by gas chromatography. Based on study findings, it was determined that the examined Dicranum species exhibit low levels of flavonoids and lipid-soluble vitamins. However, it was seen that the species under study were high in terms of phenolic acids. Particularly, it was determined that the examined species have substantial levels of gallic acid (52.96-63.1 g/g) and vanilic acid (11.96-44.83 g/g). Cinnamic acid concentration was lowest in the examined samples. The radical scavenging capacity of the examined species was found to range from 48.92% to 86.89% in 250 µl in this study. Additionally, it was discovered that the investigated species' stigmasterol contents ranged from 35.3 to 46.05 g/g. The lowest levels of ergosterol and beta-sitosterol were discovered in D. majus and D. fuscescens. Based on the results of fatty acid composition, it was discovered that oleic acid (C18:1 n9), linoelic acid (C18:2 n6), and -linolenic acid (C18:3 n3) were the predominant unsaturated fatty acids and that palmitic acid (C16:0) and behenic acid (C22:0) was the major saturated fatty acid.

Intercropping alleviated the phytotoxic effects of cinnamic acid on the root cell wall structural resistance of faba bean and reduced the occurrence of Fusarium wilt.

Soilborne Fusarium wilt is a key factor restricting the cultivation of faba bean. Intercropping faba bean and wheat effectively alleviate faba bean Fusarium wilt. This study analyzed the mechanism by which cinnamic acid promotes Fusarium wilt and the mechanism that enables intercropping alleviated Fusarium wilt. Faba beans were inoculated with Fusarium oxysporum f. sp. fabae (FOF), while the controls were not inoculated. Different concentrations of cinnamic acid were added to the inoculated plants to study the occurrence of Fusarium wilt, seedling growth, the activities of cell wall degradation enzyme (CWDESs) produced by FOF in the root, defense enzymes, total phenolics and lignin, levels of expression of the pathogenesis-related genes (PRs) PR1, PR2, and PR10, and changes in the submicroscopic cell wall structure of the roots under monocropping and intercropping systems. Cinnamic acid increased the activities of CWDEs produced by FOF in the roots, increased the activities of phenylalanine ammonia lyase and polyphenol oxidase and the contents of total phenolics and lignin, and upregulated the levels of expression of PRs in the root, but it decreased the activity of peroxidase. Transmission electron microscopy (TEM) observations identified severe damage and disruption of the root cell walls, and numerous FOF mycelia entered the cytoplasm from the cell wall. The combination of these factors increased the occurrence of Fusarium wilt. The activities of CWDEs produced by FOF in the roots decreased by intercropping wheat with faba bean, which increased the resistance of the root cell walls to infection and decreased the Fusarium wilt.

Cinnamic Acid Toxicity on the Structural Resistance and Photosynthetic Physiology of Faba Bean Promoted the Occurrence of Fusarium Wilt of Faba Bean, Which Was Alleviated Through Wheat and Faba Bean Intercropping.

BackgroundThe pattern of intercropping wheat and faba bean is an effective means to alleviate continuous cropping obstacles.AimTo study the mechanism by which cinnamic acid promotes faba bean wilt and the mechanism by which intercropping alleviates this effect.MethodsHydroponics was used to study the effects of inoculation with or without Fusarium oxysporum f. sp. fabae (FOF) and the effect of addition of different concentrations of cinnamic acid on seedling growth, Fusarium wilt, stem cell wall degrading enzyme activity, lignin content, tissue structure of the stem and leaf photosynthesis in monocropping and intercropping systems following the inoculation of faba bean with FOF.ResultsTreatment with FOF significantly reduced the biomass and leaf photosynthesis of faba bean compared with the control. Microscopic observation showed that the xylem vessels of the stem were slightly thickened. Compared with FOF alone, the combination of FOF and cinnamic acid stress significantly increased the activity of cell wall degrading enzymes (CWDEs) produced by FOF in the stem and content of lignin in the stem. Microstructural observation showed that cell wall thickening of the xylem conduit, stratification, formation of a cavity and even caused the dispersion of tissue cell structure in the stem tissue of faba bean. Furthermore, the biomass and leaf photosynthesis of faba bean decreased significantly, and the occurrence of faba bean wilt increased. Compared with the faba bean monocropping treatment, the wheat and faba bean intercropping treatment significantly reduced the activity of CWDEs of FOF produced in faba bean stems and increased the lignin content. In addition, observation of the microstructure indicated that the tissue structural cell wall thickened after the stem had decreased, and the amount of colloidal substances and their containment decreased, causing a further decrease in tissue deformation, smaller intercellular spaces, less divided layer cell damage, an increase in the aboveground biomass and leaf photosynthesis of faba bean and a decrease in the occurrence of faba bean wilt.ConclusionCinnamic acid decreased the resistance of tissue structure and promoted the occurrence of wilt. Wheat and faba bean intercropping improved the resistance of tissue structure, which reduced the occurrence of wilt.

A comparative study of TiO2 nanoparticles and Cu(II)/TiO2 nanocomposites on photodegradation of cinnamic acid (CA) under natural sunlight and artificial UV light

Surfaces of TiO2 nanoparticles decorated with Cu(II) nanoparticles as Cu(II)/TiO2 nanocomposites, were prepared via a grafting process for a comparative study on photodegradation of recalcitrant cinnamic acid (CA) in aqueous solutions. In this study, CA was used as a model representing for the wastewater from agricultural industries containing phenolic compounds. Photodegradation of CA in the solutions by TiO2 nanoparticles and Cu(II)/TiO2 nanocomposites under natural sunlight and artificial UV light, were investigated. CA concentrations in the solutions were determined from the highest peak intensities at 271 nm, detected by a UV–Vis spectrophotometer. Under 90 min of the irradiation of both light sources, CA concentrations in the solutions were degraded by Cu(II)/TiO2 nanocomposites higher and quicker than those of TiO2 nanoparticles. According to the reusability test results, both TiO2 nanoparticles and Cu(II)/TiO2 nanocomposites have potential to reuse at least three cycles. Intermediate compounds formed during the irradiation of UV light, were identified by XPS analysis confirming that CA gradually changed to be nontoxic compounds. A mechanism for the CA photodegradation pathways in the solutions by Cu(II)/TiO2 nanocomposites was proposed according to the analysis.

Properties of PLA films with cinnamic acid: Effect of the processing method

Polylactic acid (PLA) is a sustainable material for food packaging applications. The incorporation of cinnamic acid (CA) into PLA films was studied to obtain antimicrobial films, using two different techniques for film production, namely casting and thermal processing (TP). Whereas CA could be successfully incorporated at 5 and 10% w/w into TP films, only 3% w/w could be used in cast films without observing any crystallisation of CA. The acid reduced the glass transition temperature in TP films, while also improving their thermal resistance. Cast films retained a little proportion of the casting solvent which contributed to their plasticisation. The processing method greatly affected the mechanical properties of the films, with the cast films being significantly more plastic and stretchable. CA reduced the film’s stiffness and resistance to break and improved their water vapour and oxygen barrier capacity. Antibacterial assays with Listeria innocua did not show significant growth inhibition for any film, but the observed inhibition could be related to the acid concentration. This suggested that only a small amount of acid near the film surface could be released. Further research to promote the superficial concentration of cinnamic acid into PLA films is required to obtain active materials.

Phenotypic and Transcriptomic Analyses Reveal the Cell Membrane Damage of Pseudomonas fragi Induced by Cinnamic Acid.

Cinnamic acid (CA) is a safe and effective antimicrobial agent. The objective of this study was to reveal the antibacterial mechanism of CA against a food-derived Pseudomonas fragi 38-8, from the aspects of bacterial growth kinetics, cell membrane homeostasis, cell microstructure, and transcription. The minimum inhibitory concentration (MIC) of CA against P. fragi 38-8 was 0.25 mg/ml. CA retarded bacterial growth and induced a series of cell membrane changes. After CA treatment, cell membrane homeostasis was destroyed, which was evidenced by cell membrane depolarization, intracellular pH reduction, and intracellular ATPase activity decrease. Field emission scanning electron microscope (FESEM), transmission electron microscope (TEM), and confocal laser scanning fluorescence microscope (CLSM) realized the visualization of cell microstructure changes, showing cell death and morphological changes, such as cell rupture, shrinkage, and hollowness. RNA sequencing analysis further confirmed the effects of CA to the cell membrane, because of the significant enrichment of differentially expressed genes (DEGs) related to membrane. The results of the phenotype tests and RNA-seq both focused on cell membrane damage, which showed that CA exerted antibacterial effect mainly by acting on cell membrane.

Cinnamic Acid Increased the Incidence of Fusarium Wilt by Increasing the Pathogenicity of Fusarium oxysporum and Reducing the Physiological and Biochemical Resistance of Faba Bean, Which Was Alleviated by Intercropping With Wheat.

BackgroundContinuous cropping has resulted in the accumulation of self-toxic substances in faba beans which has restricted their global production. Intercropping is widely used to alleviate these problems.AimsTo explore the role of cinnamic acid stress in faba bean physiology and disease resistance, and the potential mitigating effects of intercropping the faba bean with wheat.MethodsFaba bean seedlings were grown with or without wheat in both field and hydroponic conditions in the presence of different cinnamic acid concentrations and Fusarium oxysporum (FOF), the occurrence of. Fusarium-mediated wilt and oxidative stress, as well as plant growth indices and the anti-pathogen defense system were analyzed.ResultsCinnamic acid significantly increased Fusarium pathogenicity, inhibited the activity of defense enzymes and reduced the ability of plants to resist pathogens, indicating the importance of cinnamic acid in the promotion of Fusarium wilt resulting in reduced seedling growth. Intercropping with wheat improved plant resistance by alleviating cinnamic acid-induced stress, which promoted crop growth and decreased the incidence and disease index of Fusarium wilt.ConclusionCinnamic acid promotes Fusarium wilt by stimulating pathogen enzyme production and destroying the defense capability of faba bean roots. Intercropping reduces Fusarium wilt by alleviating the damage caused by cinnamic acid to the defense system of the faba bean root system.

Transcriptome and metabolite analysis reveal the drought tolerance of foxtail millet significantly correlated with phenylpropanoids-related pathways during germination process under PEG stress

BackgroundFoxtail millet [Setaria italica (L.) P. Beauv.] is an excellent crop known for its superior level of drought tolerance across the world. Especially, less water is needed during its germination period than the other cereal crops. However, the knowledge of the mechanisms underlying the abiotic stress effects on seed germination of foxtail millet is largely unknown.ResultsThe water uptake pattern of foxtail millet seeds was ploted during germination period, according to which the germination time course of millet was separated into three phases. We sequenced the transcriptome of foxtail millet seeds, which were treated by PEG during different germination phases after sowing. The transcriptional studies revealed that more DEGs were identified during the further increase in water uptake period (phase III) than during the rapid initial uptake period (phase I) and the plateau period (phase II) under PEG stress. The pathway analysis of DEGs showed that the highly enriched categories were related to phenylpropanoid biosynthesis, plant hormone signal transduction and phenylalanine metabolism during phase III. The 20 phenylpropanoids-related genes of germinating foxtail millet were found to be down-regulated during the further increase in water uptake period under PEG stress. Further expression analysis identified 4 genes of phenylalanine ammonia-lyase, 4-coumarate-CoA ligase 3, cinnamoyl-CoA reductase 1, cationic peroxidase SPC4 in phenylpropanoids-related pathway, which played important roles in foxtail millet in response to PEG stress during different germination periods. The studies of metabolites in phenylpropanoid biosynthesis pathway revealed that higher amount of cinnamic acid was accumulated in germinating seeds under PEG stress, while the contents of p-coumaric acid, caffeic acid, ferulic acid and sinapic acid were decreased. And the effects of five phenolic compounds on germination and growth of foxtail millet showed that 1 mM concentration of cinnamic acid inhibited shoot and root growth, especially root development. Ferulic acid, caffeic acid, sinapic acid and p-coumaric acid could increase the root length and root/sprout in lower concentration.ConclusionsThese findings suggest that key genes and metabolites of foxtail millet related with phenylpropanoids pathway may play prominent roles in the regulation of resistance to drought during germination. Foxtail millet can probably avoid drought by regulating the levels of endogenous allelochemicals.

A Novel Cinnamic Acid‐Based Organogel: Effect of Oil Type on Physical Characteristics and Crystallization Kinetics

AbstractOil sources (canola, sunflower, and flax‐seed oil) characterized by unsaturated fatty acids are gelled by using cinnamic acid. The physical characteristics and crystallization kinetics of cinnamic acid‐based organogels are investigated. A phase diagram with cinnamic acid concentrations ranging from 1% to 7% (w/w) shows that both canola and sunflower oil organogels are formed at 3.0% (w/w) cinnamic acid at 5 °C. The flax‐seed oil organogels are formed at 4.0% (w/w) at 5 °C. Firmness is shown to be dependent on the fatty acid composition and viscosity of the oil. Flax‐seed oil with a higher degree of unsaturation and lower viscosity tends to produce harder organogels. This result is consistent with the observations of polarized light microscopy. The organogels have low solid fat content at 35 °C which is close to the human body temperature, and no effect of oil type is found. The X‐ray diffraction measurements show β'‐form crystal exists in three types of organogels. The thermal properties vary in different types of organogels. The crystallization kinetics results suggest that three types of organogels crystallize by 1D and 2D mixed growth and instantaneous nucleation.Practical Applications: These findings provide in‐depth characteristics of cinnamic acid‐based organogels, which are a substitute for solid fats.

Effect of Calcium Carbonate Residues from Cement Industries on the Phenolic Composition and Yield of Shiraz Grapes

Phenolic compounds are secondary metabolites synthesised in response to biotic or abiotic stress in plants. This stress-induced increase in phenolic compound concentrations is generally activated by internal levels of abscisic acid (ABA). The exogenous application of ABA or calcium chloride on grapevines is also known to increase grape yield and alter the phenolic composition of grapes. Residues of cement industries such as calcium carbonates (CaCO3) are a safe environmental source of calcium that could be used to induce the synthesis of phenolic compounds and act as a yield promoter in grapes and other crops. Consequently, the objective of this study was to evaluate the effect of cement industries’ CaCO3 residues (CaCO3R) on the yield and concentration of phenolic compounds in Shiraz grapes. Thirteen phenolic compounds were identified and quantified by HPLC-DAD. Malvidin-3-O-glucoside was the major anthocyanin found in Shiraz grapes, and its concentration increased by more than 200% in CaCO3R-treated vines. Similarly, the concentration of cinnamic acid, the main precursor of phenolic compounds, increased by more than 900% in grapes treated with CaCO3 residues at harvest time. Finally, catechin, epicatechin and procyanidin B1 and B2 increased significantly at harvest time in CaCO3R-treated grapes relative to the controls. In general, it was found that foliar application of CaCO3 residues from the cement industry at veraison induced an increase in yield, and in the concentration and composition of phenolic compounds in grapes.

Intercalations and Characterization of Zinc/Aluminium Layered Double Hydroxide-Cinnamic Acid

Cinnamic acid (CA) is known to lose its definite function by forming into radicals that able to penetrate into the skin and lead to health issues. Incorporating CA into zinc/aluminum-layered double hydroxides (Zn/Al-LDH) able to reduce photodegradation and eliminate close contact between skin and CA. Co-precipitation or direct method used by using zinc nitrate hexahydrate and aluminium nitrate nonahydrate as starting precursors with addition of various concentration of CA. The pH were kept constant at 7 ± 0.5. Fourier Transform Infrared-Attenuated Total Reflectance (FTIR-ATR) shows the presence of nanocomposites peak 3381 cm–1 for OH group, 1641 cm–1 for C=O group, 1543 cm–1 for C=C group and 1206 cm–1 for C–O group and disappearance of N–O peak at 1352 cm–1 indicates that cinnamic acid were intercalated in between the layered structures. Powder X-Ray Diffraction (PXRD) analysis for Zn/Al-LDH show the basal spacing of 9.0 Ǻ indicates the presence of nitrate and increases to 18.0 Ǻ in basal spacing in 0.4M Zn/Al-LDH-CA. CHNS analysis stated that 40 % of cinnamic acid were being found and intercalated in between the interlayer region of the Zn/Al-LDH with higher thermal stability. Field Emission Scanning Electron Microscope (FESEM) images of 0.4 M Zn/Al-LDH-CA shows that the nanocomposites are in more compact, flaky non porous, large agglomerates with smooth the surfaces of the intercalated compound. Controlled release was successful with 80 % release in phosphite anion and 70 % release carbonate anion. The cinnamic acid was successfully inserted between the interlayer regions of Zn/Al-LDH with slow release formulation.

Antibacterial activity and mechanism of cinnamic acid and chlorogenic acid against Alicyclobacillus acidoterrestris vegetative cells in apple juice

SummaryThe aim of this study was to investigate the antibacterial activity and mechanism of cinnamic acid and chlorogenic acid against Alicyclobacillus acidoterrestris. Minimum inhibitory concentrations of cinnamic acid and chlorogenic acid were 0.375 and 2.0 mg mL−1, and the minimum bactericidal concentrations were 0.50 and 4.0 mg mL−1, respectively. The apple juice ingredients had little influence on the inactivation of A. acidoterrestris. After treatment with cinnamic acid and chlorogenic acid, the morphology of A. acidoterrestris cells were severely destroyed; the leakage of nucleic acids and proteins increased significantly. SDS‐PAGE investigation of bacterial proteins proved that the loss of soluble proteins was obvious as well. These results demonstrated that cinnamic acid and chlorogenic acid exerted their antibacterial activity mainly by an action mode of membrane disruption. This study provides an alternative method for the control of A. acidoterrestris‐related spoilage in the fruit juice/beverage industry.

Enhancement of biocontrol efficacy of Cryptococcus laurentii by cinnamic acid against Penicillium italicum in citrus fruit

Cinnamic acid was effective to control blue mold caused by Penicillium italicum in ‘Orah’ mandarins. The inhibition of fruit decay was positively correlated with cinnamic acid concentration. Cinnamic acid at 1.5 mM, in combination with the biocontrol yeast Cryptococcus laurentii at 1 × 107 cells/mL, provided synergistic effects against P. italicum. Population dynamic analysis demonstrated that the growth of C. laurentii in fruit rind wounds was not significantly influenced by cinnamic acid. C. laurentii multiplied rapidly, regardless of whether the yeast was used alone or combined with cinnamic acid. By contrast, spore germination and mycelial growth of P. italicum in the culture medium were markedly inhibited by cinnamic acid. By using propidium iodide fluorescent staining, loss of membrane integrity in P. italicum was observed after cinnamic acid treatment. Furthermore, cinnamic acid led to the leakage of cellular constituents including soluble proteins and carbohydrates from hyphae of P. italicum. These results indicated that the fungal pathogen P. italicum is more sensitive to cinnamic acid than the biocontrol yeast C. laurentii. Taken together, our data suggest that the enhancement in biocontrol efficacy of C. laurentii might be associated with the differential influence of cinnamic acid on the antagonistic yeast and the fungal pathogen.