Concentration Of Benzoic Acid Research Articles

Investigation of benzoic acid and sorbic acid concentrations in tomato paste, pepper paste, ketchup, mayonnaise, and barbeque sauce samples by headspace gas chromatography-mass spectrometry

Investigation of benzoic acid and sorbic acid concentrations in tomato paste, pepper paste, ketchup, mayonnaise, and barbeque sauce samples by headspace gas chromatography-mass spectrometry

DETERMINATION AND ESTIMATION OF PRESERVATIVES IN PACKAGED FOOD

Objectives: Packaged food and beverages are consumed all over the world for their nutritional value, durability, their quenching properties, stimulating effect, and for their medicinal value. Food additives are substances that are added to food or animal feed during processing or storage. They include antioxidants, preservatives, coloring and flavoring agents, and anti-infective agents. This study focuses on the determination of the content levels of sodium benzoate in different samples of commercially available food products in the local market. Methods: Food products selected are Sting Energy Drink, Listerine Cool Mint Mouthwash, Kissan Fresh Tomato Ketchup, and Dabur Hommade Imli Sauce. Identification tests for the presence of benzoic and its salts in food products were conducted. A standard graph for benzoic acid at different concentrations is drawn. The concentrations of benzoic acid in selected food products were determined and evaluated by rapid and simple ultraviolet spectrophotometric methods. Results: The results obtained from this study indicated that the quantity of benzoic acid in analyzed food samples was in the range of 18.3– 160.5 μg/mL. Conclusion: The optimum benzoic acid concentration to be in marketed food preservatives is 20–700 μg/mL. As the benzoic concentration in selected food products determined were in range, we found that all the selected samples are safe to use.

HPLC Method for the Determination of Benzoic acid in samples of commercial children’s food in the Markets of Aden -Yemen

The objective of this study was to determine the quantities of benzoic acid in some local and imported food products for the distributed children in Aden, Yemen. Samples of different food products for children (32) using HPLC method, were collected in August 2022, from different supermarkets in Aden, Yemen. Chromatographic separation was achieved using a C-18 column (EXCGL 120 ODS-A) and acetate buffer pH=4.4 - methanol (65:35) as mobile phase, 1 ml/min flow rate and UV detection at 233 nm. The results show that quantities of benzoic acid in studied samples varies greatly starting from 1.778 ppm to 849.295ppm, All samples of group II beverages and Juices samples and group III jam samples were under the study contain concentrations of benzoic acid within the limits allowed by standards of Yemeni and World Health Organization and the estimated maximum allowable benzoic acid in beverages, Juices and jam to 1000 ppm. All samples of the groups I and V cakes and food for children mainly manufacturers of grain, The samples that were under the study contain concentrations of benzoic acid and it is difficult to compare the amount of benzoic acid in samples of two groups with international standards, regional and local non-existence. All samples of the group IV dry milk and cheese samples under study contain concentrations of benzoic acid within the limits allowed according to the standard specifications of Yemen and specifications of the World Health Organization (WHO), which determined the maximum allowable of benzoic acid in dry milk at 50 ppm and the maximum allowed for benzoic acid in the cheese is 2000 ppm, except for sample No. 28 contains a concentration is higher than the allowable limit.

Carboxylic acid-induced freshwater acidification effects on behavior and operculum ultrastructure in the gastropod mollusc Bellamya bengalensis

ABSTRACT Global freshwater acidification, accelerated by rising pCO2, remains a critical ecological concern. Despite the prevalence of carboxylic acids like acetic acid (AA) and benzoic acid (BA) in freshwater, their potential to exacerbate acidification and impact biota is not fully understood. In this study, we examined behavioural disruptions during acute exposures (96 h) and operculum ultrastructure changes during chronic exposures (28 days) to ecologically-relevant concentrations of AA (39.7, 79.5, and 99.4 mg/L) and BA (31.4, 62.8, and 78.5 mg/L) in Bellamya bengalensis. Acute exposures revealed significant behavioural disruption, including reduced activity and impaired escape behaviour. Chronic exposures led to ultrastructural defects in the operculum, with more pronounced effects in BA-exposed groups. Acidification scenarios appeared to impact calcium carbonate crystal formation, compromising opercula structure. Our study underscores the importance of understanding both acute and chronic effects of freshwater acidification on molluscan species, emphasising the need for further research into the underlying mechanisms and thresholds of acidification tolerance.

Phenolic Acids and Flavonoids Play Important Roles in Flower Bud Differentiation in Mikania micrantha: Transcriptomics and Metabolomics

Mikania micrantha is a highly invasive vine, and its ability to sexually reproduce is a major obstacle to its eradication. The long-distance dissemination of M. micrantha depends on the distribution of seeds; therefore, inhibiting M. micrantha flowering and seed production is an effective control strategy. The number of blooms of M. micrantha differs at different altitudes (200, 900, and 1300 m). In this study, we used a combination of metabolomics and transcriptomics methods to study the patterns of metabolite accumulation in the flower buds of M. micrantha. Using LC-MS/MS, 658 metabolites were found in the flower buds of M. micrantha at three different altitudes (200, 900, and 1300 m). Flavonoids and phenolic acids were found to be the main differential metabolites, and their concentrations were lower at 900 m than at 200 m and 1300 m, with the concentrations of benzoic acid, ferulic acid, and caffeic acid being the lowest. The biosynthesis pathways for flavonoids and phenolic compounds were significantly enriched for differentially expressed genes (DEGs), according to the results of transcriptome analysis. The production of flavonoid and phenolic acids was strongly linked with the expressions of phenylalanine ammonia-lyase (PAL), caffeoyl-CoA O-methyltransferase (COMT), and 4-coumarate-CoA ligase (4CL), according to the results of the combined transcriptome and metabolome analysis. These genes’ roles in the regulation of distinct phenolic acids and flavonoids during M. micrantha bud differentiation are still unknown. This study adds to our understanding of how phenolic acids and flavonoids are regulated in M. micrantha flower buds at various altitudes and identifies regulatory networks that may be involved in this phenomenon, offering a new approach for the prevention and management of M. micrantha.

Benzoic acid promotes Fusarium wilt incidence by enhancing susceptibility and reducing photosynthesis of faba bean

AbstractContinuous faba bean (Vicia faba L.) cropping causes adverse effects, including increased secretion of autotoxic substances (benzoic acid) and incidence of diseases (Fusarium wilt). Fusarium commune infects faba bean seedlings and is treated with different benzoic acid concentrations. Therefore, this investigation analysed the association of benzoic acid with faba bean wilt incidence and the mechanisms involved. Furthermore, seedling growth, tissue structure, Fusarium wilt incidence, cell wall degrading enzymes (CWDEs) activity, lignin levels, and leaf photosynthetic parameters were assessed. The results indicated that fungal infection markedly reduced biomass and photosynthesis in the faba bean. Microscopic analysis showed slightly thickened stems' xylem vessels. Benzoic acid treatment with F. commune infection substantially increased F. commune‐mediated CWDEs activity in the faba bean stem and lignin level but reduced photosynthesis and biomass, elevating Fusarium wilt incidence. Furthermore, broken tissues, xylem thickening, and stem cavities were observed. The investigation indicated that benzoic acid treatment and F. commune infection‐induced stem lignin might be a resistance response; however, increased pathogenicity and reduced photosynthesis enhanced susceptibility and aggravated Fusarium wilt.

The effect of water molecules and air humidity on the cluster formation from benzoic acid with sulfuric acid/ammonia/dimethylamine: A molecular-scale investigation

In the atmosphere, benzoic acid (BA) is one of the most abundant aromatic acids and contributes to new particle formation (NPF). Sulfuric acid (SA), ammonia (NH3), and dimethylamine (DMA) have been identified as critical nucleation precursors. Since water (H2O) is abundant in the atmosphere, the hydration effect is a significant concern. However, the hydration effect on the precise mechanisms of cluster formation remains unclear. In this study, theoretical calculation method was used to investigate the interactions between BA and NH3/DMA/SA in the presence of up to six water molecules at the PW91PW91/6-311++G(3df,3pd) using geometrical analysis, topological analysis, and the Gibbs free energies analysis. Hydrated distributions of clusters under different relative humidity (RH) levels and their atmospheric implications were also estimated. This study shows water molecules have mild impacts on the interaction between BA and NH3/DMA, but have relatively strong effect on the interaction between BA and SA. Hydrated distributions for the three types of clusters show that they are insensitive to different RH levels, and a completely unhydrated (BA)(NH3) cluster dominates the hydrated distributions. When the concentrations of BA are particularly high, especially in heavily polluted areas, the concentrations of (BA)(SA) can reach the same order of magnitude or higher than those of (SA)2. Thus, the importance of BA in prompting NPF has been revealed to a certain extent.

Low doses of glyphosate do not damage the secondary metabolism of common bean

Glyphosate application, even in low doses, changes the metabolism of crops. This research aimed to evaluate the effects of glyphosate low doses and sowing season on metabolic changes of early-cycle common beans. Two experiments were conducted in the field, one in the winter season and one in the wet season. The experimental design was a randomized complete block design consisting of the application of glyphosate low doses [0.0, 1.8, 7.2, 12.0, 36.0, 54.0, and 108.0 g acid equivalent (a.e.) ha−1] in the phenological stage V4 with four replications. In the winter season, glyphosate and shikimic acid were increased five days after the application of treatments. In contrast, the same compounds increased only at doses of 36 g a.e. ha−1 and above in the wet season. The dose of 7.2 g a.e. ha−1 increased phenylalanine ammonia-lyase and benzoic acid in the winter season. The doses of 54 and 108 g a.e. ha−1 increased benzoic acid, caffeic acid, and salicylic acid. Our study indicated that glyphosate low doses increase the concentration of shikimic, benzoic, salicylic and caffeic acid, PAL and tyrosine. There was no reduction in aromatic amino acids and in secondary compounds from the shikimic acid pathway.

Synergistic Antibacterial Screening of Cymbopogon citratus and Azadirachta indica: Phytochemical Profiling and Antioxidant and Hemolytic Activities.

Current studies were performed to investigate the phytochemistry, synergistic antibacterial, antioxidant, and hemolytic activities of ethanolic and aqueous extracts of Azadirachta indica (EA and WA) and Cymbopogon citratus (EC and WC) leaves. Fourier transform infrared data verified the existence of alcoholic, carboxylic, aldehydic, phenyl, and bromo moieties in plant leaves. The ethanolic extracts (EA and EC) were significantly richer in phenolics and flavonoids as compared to the aqueous extracts (WA and WC). The ethanolic extract of C. citratus (EC) contained higher concentrations of caffeic acid (1.432 mg/g), synapic acid (6.743 mg/g), and benzoic acid (7.431 mg/g) as compared to all other extracts, whereas chlorogenic acid (0.311 mg/g) was present only in the aqueous extract of A. indica (WA). Food preservative properties of C. citratus can be due to the presence of benzoic acid (7.431 mg/g). -Gas chromatography-mass spectrometry analysis demonstrated the presence of 36 and 23 compounds in A. indica and C. citratus leaves, respectively. Inductively coupled plasma analysis was used to determine the concentration of 26 metals (Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Mo, Na, Ni, Pb, Sb, Se, Si, Sn, Sr, V, Zn, Zr, Ti); the metal concentrations were higher in aqueous extracts as compared to the ethanolic extracts. The extracts were generally richer in calcium (3000-7858 ppm), potassium (13662-53,750 ppm), and sodium (3181-8445 ppm) and hence can be used in food supplements as a source of these metals. Antioxidant potential (DDPH method) of C. citratus ethanolic extract was the highest (74.50 ± 0.66%), whereas it was the lowest (32.22 ± 0.28%) for the aqueous extract of A. indica. Synergistic inhibition of bacteria (Staphylococcus aureus and Escherichia coli) was observed when the aqueous extracts of both the plants were mixed together in certain ratios (v/v). The highest antibacterial potential was exhibited by the pure extract of C. citratus, which was even higher than that of the standard drug (ciprofloxacin). The plant extracts and their mixtures were more active against S. aureus as compared to E. coli. No toxic hemolytic effects were observed for the investigated extracts indicating their safe medicinal uses for human beings.

Preparation of sorbents for solid-phase extraction of benzoic acid from aqueous solutions

Aim to obtain sorbents based on ethylcellulose for solid-phase extraction of benzoic acid from aqueous solutions.
 Material and methods. To create the sorbents for solid-phase extraction, we used the following reagents: ethyl cellulose, copper sulfate pentahydrate, sodium hydroxide, benzoic acid, purified water, ethyl alcohol 95%. The samples of ethyl cellulose-based sorbents were studied by Fourier transform IR spectrometry on an Agilent Cary 630 FTIR spectrometer. The Agilent Microlab PC Expert software was used for registration and primary processing of IR spectra, and for statistical analysis. The capacity of sorbents for solid-phase extraction was evaluated in a dynamic mode. The microstructure of the samples was studied by field emission scanning electron microscopy (FE-SEM) on a Hitachi SU8000 electron microscope. The specific surface area of the synthesized sorbents was measured by low-temperature nitrogen porometry using the Brunauer Emmett Teller approximation, on the Autosorb 1 device, using the Quantachrome AS1Win software, according to a well-known technique.
 Results. А method was proposed for obtaining a sorbent for solid-phase extraction of benzoic acid from aqueous solutions. The IR-spectrometry did not reveal significant differences between ethylcellulose samples and the obtained sorbent samples. The optimal concentrations of ethylcellulose and benzoic acid in the reaction mixture were determined to create a sorbent with a maximum capacity of 19.2 g/g. The differences in the morphological structure of the surface of ethylcellulose matrix and obtained sorbents were described. The specific surface area of ethylcellulose sorbent with a maximum capacity for benzoic acid was 14.10 cm2/g.

Application of Pyrolysis for the Evaluation of Organic Compounds in Medical Plastic Waste Generated in the City of Cartagena-Colombia Applying TG-GC/MS.

In this study, the thermal degradation and pyrolysis of hospital plastic waste consisting of polyethylene (PE), polystyrene (PS), and polypropylene (PP) were investigated using TG-GC/MS. The identified molecules with the functional groups of alkanes, alkenes, alkynes, alcohols, aromatics, phenols, CO and CO2 were found in the gas stream from pyrolysis and oxidation, and are chemical structures with derivatives of aromatic rings. They are mainly related to the degradation of PS hospital waste, and the alkanes and alkenes groups originate mainly from PP and PE-based medical waste. The pyrolysis of this hospital waste did not show the presence of derivatives of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans, which is an advantage over classical incineration methodologies. CO, CO2, phenol, acetic acid and benzoic acid concentrations were higher in the gases from the oxidative degradation than in those generated in the pyrolysis with helium. In this article, we propose different pathways of reaction mechanisms that allow us to explain the presence of molecules with other functional groups, such as alkanes, alkenes, carboxylic acids, alcohols, aromatics and permanent gases.

Minimum Inhibitory Concentration (MIC) of Propionic Acid, Sorbic Acid, and Benzoic Acid against Food Spoilage Microorganisms in Animal Products to Use MIC as Threshold for Natural Preservative Production.

Some preservatives are naturally contained in raw food materials, while in some cases may have been introduced in food by careless handling or fermentation. However, it is difficult to distinguish between intentionally added preservatives and the preservatives naturally produced in food. The objective of this study was to evaluate the minimum inhibitory concentration (MIC) of propionic acid, sorbic acid, and benzoic acid for inhibiting food spoilage microorganisms in animal products, which can be useful in determining if the preservatives are natural or not. The broth microdilution method was used to determine the MIC of preservatives for 57 microorganisms. Five bacteria that were the most sensitive to propionic acid, benzoic acid, and sorbic acid were inoculated in unprocessed and processed animal products. A hundred microliters of the preservatives were then spiked in samples. After storage, the cells were counted to determine the MIC of the preservatives. The MIC of the preservatives in animal products ranged from 100 to 1,500 ppm for propionic acid, from 100 to >1,500 ppm for benzoic acid, and from 100 to >1,200 ppm for sorbic acid. Thus, if the concentrations of preservatives are below the MIC, the preservatives may not be added intentionally. Therefore, the MIC result will be useful in determining if preservatives are added intentionally in food.

Effect of Corn Straw on Hydrogen Production from Lignite

The conversion of lignite to clean energy has won considerable attention and plays an important role in achieving the goal of carbon reduction. The effects of corn straw on hydrogen production from lignite was explored by using lignite as the substrate and corn straw as an exogenous substance. The fermentation mechanism was elucidated through the analysis of total and daily hydrogen production; the concentration of humic acid, benzoic acid, pyruvate, and glucose, as well as pH value. In addition, total solid (TS), and volatile solid (VS) from activated sludge before and after fermentation are examined. The results showed that corn straw could accelerate hydrogen production from lignite with an optimal content of corn straw of 40%. The fermentative hydrogen production with 40% corn straw was up to 186.20 mL, 3.40 times higher than that of the control group. Corn straw effectively improved the concentration of humic acid and benzoic acid, accelerating the anaerobic fermentation of lignite to produce hydrogen. The concentration of pyruvic acid, glucose, pH, and the changes in TS and VS before and after fermentation showed that the group of 40% corn straw had a better promotion effect than other systems for hydrogen production. This provides a new idea for improving hydrogen production through lignite anaerobic fermentation.

Faba Bean-Wheat Intercropping Can Control the Occurrence of Faba Bean Fusarium Wilt by Alleviating the Inhibitory Effect of Benzoic Acid on Disease Resistance Metabolism and the Expression of Resistance Genes.

Background: Continuous cropping leads to the accumulation of autotoxic substances in faba beans, which limits their global production. Intercropping is widely used to alleviate these problems. Aim: This study aims to explore the important role of Fusarium oxysporum f. sp. fabae (FOF) and benzoic acid stress in enhancing the occurrence of faba bean Fusarium wilt and the potential mechanism of faba bean-wheat intercropping to control the occurrence of this disease. Methods: We analyzed the pathogenic mechanism of FOF and benzoic acid and the defense response of faba bean-wheat intercropping against the autotoxicity of benzoic acid under hydroponic conditions that included the pathogen alone and in combination with different concentrations of benzoic acid. Results: The dual stress of FOF and benzoic acid inhibited the activity of defensive enzymes, the synthesis of defensive substances, and the expression of defensive genes in faba bean roots and reduced the disease resistance of faba bean. This shows that benzoic acid plays an important role in helping FOF cause disease. Faba bean-wheat intercropping improves plant resistance by alleviating benzoic acid stress and reducing the incidence and disease index of Fusarium wilt. Conclusion: The dual stress of FOF and benzoic acid promotes the occurrence of faba bean Fusarium wilt by destroying the root defense system of faba bean. Faba bean-wheat intercropping can effectively alleviate the autotoxicity of benzoic acid and control the occurrence of Fusarium wilt by improving the physiological and biochemical resistance of faba beans and the expression of defense genes.

Экстракция кадмия в расслаивающейся системе дифенилгуанидин – бензойная кислота – вода

An delaminating system with diphenylguanidine and benzoic acid without an organic solvent was studied in order to evaluate its effectiveness in relation to the cadmium ions extraction. Extraction was studied in the presence of an inorganic (hydrochloric) acid or inorganic bases (sodium or ammonium hydroxide), as well as inorganic complexing agents (KI, NH4SCN). The optimal conditions for the cadmium extraction have been determined. It is shown that the most efficient cadmium extraction was observed when sodium hydroxide was introduced into the delaminating system. It has been established that with an increase in the concentrations of both diphenylguanidine and benzoic acid, the cadmium extraction increases. It was found that the quantitative extraction of cadmium was observed only in the presence of an inorganic complexing agent – potassium iodide.

Interference of endogenous benzoic acid with the signatures of sulfonic acid derivatives and carbohydrates in fermented dairy products

Endogenous benzoic acid causes detrimental effects on public health, but the underlying mechanisms often remain elusive. Benzoic acid (0.00–40.00 mg L − 1) was detected from sixty fermented goat milk samples in six replicates, indicating the existence of endogenous benzoic acid. Herein, we investigated the effects of benzoic acid on the variations of metabolome and proteome signatures in fermented goat milk via integrative metabolomics (LOQ 2.39–98.98 μg L − 1) and proteomics approach based on UHPLC-Q-Orbitrap HRMS. Explicitly, benzoic acid reduced the content of taurine (7.06–4.80 mg L − 1) and hypotaurine (3.86–1.74 mg L − 1) due to a significant decrease in the levels of glutamate decarboxylase 1 by benzoic acid. The reduction in lactose (7.13–5.31 mg L − 1) and d-galactose (4.39–3.37 mg L − 1) content was related to the decrease in α-lactalbumin and β-galactosidase levels, respectively, in fermented goat milk containing 40.00 mg L − 1 benzoic acid. Meanwhile, the levels of maltose (22.84–16.53 mg L − 1) and raffinose (4.19–3.10 mg L − 1) progressively decreased with increasing benzoic acid concentrations (0.00–40.00 mg L − 1), which had detrimental effects on the nutritional quality of fermented goat milk. Additionally, the concentration of benzoic acid and fermentation temperature are the most important factors to control the loss of nutrients in fermented dairy products.

Intercropping of wheat alleviates the adverse effects of phenolic acids on faba bean.

After years of continuous cultivation of faba beans (Vicia faba L.), autotoxic substances accumulate in the soil, leading to a high incidence of Fusarium oxysporum (FOF) wilt. Faba bean-wheat intercropping is often used to alleviate these problems. The goal of this research was to explore the role of benzoic acid and cinnamic acid in promoting the occurrence of faba bean Fusarium wilt and the potential mechanism of faba bean-wheat intercropping to control the occurrence of this disease. We established a field experiment and a hydroponic experiment that involved the inoculation of FOF and the exploration of exogenous addition of cinnamic acid and benzoic acid at different concentrations, the effects on the degree of peroxidation, resistance system, and ultrastructure of faba bean roots. In addition, the antioxidative response of faba bean-wheat intercropping against the autotoxicity of benzoic acid and cinnamic acid was examined. In the field experiment, compared with monoculture, faba bean-wheat intercropping effectively controlled the occurrence of Fusarium wilt, significantly reduced the contents of H2O2 and O2 - in faba bean roots, increased the expression and activity of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), maintained cell stability, and significantly reduced the contents of benzoic acid and cinnamic acid in faba bean rhizosphere. In the pot experiment, it was found that compared with the control, different concentrations of benzoic acid and cinnamic acid (50, 100, and 200 mg·L-1) significantly increased the content of H2O2 and O2 - in faba bean, decreased the activity and gene expression of antioxidant enzymes SOD and CAT, and damaged cell membrane structure. Furthermore, it promoted the occurrence of Fusarium wilt of faba bean. The faba bean-wheat intercropping alleviated the stress. Benzoic acid and cinnamic acid can increase the content of hydrogen peroxide and superoxide anions in faba bean plants, reduce the enzymatic activity and expression of antioxidant enzyme genes, damage the cell membrane structure, and promote the occurrence of faba bean Fusarium wilt. The faba bean-wheat intercropping can effectively alleviate the autotoxicity of benzoic acid and cinnamic acid and reduce the occurrence of faba bean Fusarium wilt.

Antimicrobial effect of a drinking water additive comprising four organic acids on Campylobacter load in broilers and monitoring of bacterial susceptibility

Application of organic acids via feed or drinking water is under discussion as a possible intervention strategy to reduce Campylobacter (C.) load in primary poultry production. A previous in vitro study showed that reduced concentrations of sorbic acid, benzoic acid, propionic acid, and acetic acid were required for antibacterial activity against Campylobacter when using a mixture of these 4 acids compared to when using the single acids. The present study aimed at determining the antibacterial efficiency of this combination in vivo as a drinking water additive for reducing shedding and intestinal C. jejuni colonization in broilers. Furthermore, we assessed whether the inoculated C. jejuni strain BfR-CA-14430 adapted in vivo to the applied organic acids. Results of this study showed that adding the organic acids consistently reduced Campylobacter loads in cloacal swabs. While significant reductions were observed within the entire study period, a maximum 2 log reduction occurred at an age of 18 d. However, after dissection at the end of the trial, no significant differences were detected in Campylobacter loads of cecal and colon contents compared to the control group. Susceptibility testing of re-isolates from cloacal swabs and cecal content revealed equal minimum inhibitory concentration (MIC) values compared to the inoculated test strain, suggesting that C. jejuni remained susceptible throughout the trial.

Endogenous benzoic acid interferes with the signatures of amino acids and thiol compounds through perturbing N-methyltransferase, glutamate-cysteine ligase, and glutathione S-transferase activity in dairy products

Endogenous benzoic acid causes adverse effects on individual health, but the potential mechanisms often remain elusive. The positive rate of benzoic acid in seventy-two goat milk samples in triplicate was 93.6 %, verifying the presence of endogenous benzoic acid. In this study, we investigated the differences in protein expression and metabolites among goat milk with different final concentrations of benzoic acid via combined proteomics and metabolomics (LOQ 3.25 to 56.63 μg L-1) analysis based on UHPLC-Q-Orbitrap HRMS. Integrated analysis showed that benzoic acid reduced the content of l-histidine (from 1.27 to 0.49 mg/L) and 1-methylhistidine (from 1.40 to 0.68 mg/L), due to the increase of benzoic acid (0–30 mg/L) concentration significantly reduced the level and activity of N-methyltransferase. Protein-metabolite interactions suggested that benzoic acid enhanced glutamate-cysteine ligase and glutathione S-transferase expression and affected l-glutamate (from 1.22 to 0.49 mg/L) and glutathione contents, eventually leading to the formation of off-flavors and oxidation of goat milk. Meanwhile, the level of l-phenylalanine (from 4.17 to 1.94 mg/L) and l-tyrosine (from 1.05 to 0.26 mg/L) progressively decreased with the increase of benzoic acid concentration, which had a deleterious effect on the nutritional value and flavor formation of goat milk. These findings clarified the mechanism by which low-dose benzoic acid negatively affects the nutritional quality and flavor formation of goat milk.

Efficacy of fungicide alternatives against late wilt disease of maize and their influence on plant morphogenesis and yield characters

Summary Efficiency of various organic acids, organic salts, essential oils, algae (an extract of Chlorella vulgaris and a commercial product), and bioagents against Magnaporthiopsis maydis, causing maize late wilt disease, was evaluated in laboratory and field conditions. For the in vitro tests, isolated M. maydis field strains from Egypt were used. Additionally, in field experiments different application methods were tested for their efficacy throughout two successive growing seasons. Results showed maximum growth inhibition of M. maydis at different concentrations of salicylic acid, ascorbic acid, benzoic acid and humic acid as well as sodium benzoate, potassium sorbate, di-potassim phosphate and calcium choloride, in descending order. The essential oils of carnation, lemongrass and black seed followed a similar trend. The minimum pathogenic fungal growth was achieved when the pathogen was exposed to the antagonistic Trichoderma viride followed by Trichoderma harzianum, Bacillus subtilis and Pseudomonas fluorescens treatments. Under field conditions, the highest disease reduction was recorded after treatment with salicylic acid (all application methods), humic acid (all application methods), sodium benzoate (seed dressing), carnation oil (seed dressing or seed dressing +foliar spray) and the bioagents B. subtilis and P. fluorescens (soil drench). Overall, all treatments and all application methods led to significant lower disease incidence compared to the untreated control in both growing seasons. Additionally, all treatments achieved an enhancement of plant morpho-genesis and yield characters. The most effective combinations of compounds/bioagents and application methods can be considered for future use in IPM management of late wilt disease of maize.