CON Trial Research Articles

Effect of Fluid Intake on Acute Changes in Plasma Volume: A Randomized Controlled Crossover Pilot Trial.

Plasma volume (PV) undergoes constant and dynamic changes, leading to a large intra-day variability in healthy individuals. Hydration is known to induce PV changes; however, the response to the intake of osmotically different fluids is still not fully understood. In a randomized controlled crossover trial, 18 healthy individuals (10 females) orally received an individual amount of an isotonic sodium-chloride (ISO), Ringer (RIN), or glucose (GLU) solution. Hemoglobin mass (Hbmass) was determined with the optimized carbon monoxide re-breathing method. Fluid-induced changes in PV were subsequently calculated based on capillary hemoglobin concentration ([Hb]) and hematocrit (Hct) before and then every 10 minutes until 120 min (t0-120) after the fluid intake and compared to a control trial arm (CON), where no fluid was administered. Within GLU and CON trial arms, no statistically significant differences from baseline until t120 were found (p > 0.05). In the ISO trial arm, PV was significantly increased at t70 (+138 mL, p = 0.01), t80 (+191 mL, p < 0.01), and t110 (+182 mL, p = 0.01) when compared to t0. Moreover, PV in the ISO trial arm was significantly higher at t70 (p = 0.02), t110 (p = 0.04), and t120 (p = 0.01) when compared to the same time points in the CON trial arm. Within the RIN trial arm, PV was significantly higher between t70 and t90 (+183 mL, p = 0.01) and between t110 (+194 mL, p = 0.03) and t120 (+186 mL, p < 0.01) when compared to t0. These results demonstrated that fluids with a higher content of osmotically active particles lead to acute hemodilution, which is associated with a decrease in [Hb] and Hct. These findings underpin the importance of the hydration state on PV and especially on PV constituent levels in healthy individuals.

Effect of High-Intensity With Short-Duration Re-Warm-Up on Subsequent Performance in a Cold Environment.

Yamashita, Y and Umemura, Y. Effect of high-intensity with short-duration re-warm up on subsequent performance in a cold environment. J Strength Cond Res 38(6): e280-e287, 2024-The aim of this study was to investigate the effect of high-intensity, short-duration re-warm-up (RW) during half time (HT) on subsequent performance in a cold environment. Eleven male subjects (age, 21 ± 2 years; height, 172.4 ± 4.5 cm; body mass, 65.6 ± 7.1 kg; V̇ o2 max, 47.5 ± 4.8 ml·kg -1 ·min -1 ) performed 2 experimental trials comprising 40 minutes of intermittent cycling exercise, which consisted of 15-second rest, 25-second unloading cycling, 10-second high-intensity cycling, and 70-second moderate-intensity cycling as the first half. In the second half, a cycling intermittent-sprint protocol (CISP) was performed, separated by a 15-minute HT period in cold conditions (5 °C, 50% relative humidity). Two experimental trials were included in a random order: (a) approximately 1 minute of high-intensity, short-duration RW (3 sets of 3-second maximal pedaling [body weight × 0.075 kp]) trial high-intensity intermittent cycling trials (HII); (b) 15 minutes of seated rest trial (CON). Cycling intermittent-sprint protocol consisted of 10 sets of a 2-minute exercise protocol, and each set consisted of 10-second rest, 5-second maximal pedaling (body weight × 0.075 kp), and 105-second active recovery at 50% maximum oxygen uptake (V̇ o2 max). Peak power output of 5-second maximal pedaling during CISP was higher in HII trials than in CON trials (HII: 807 ± 81 W, CON: 791 ± 78 W, p < 0.05). There was no significant difference in rectal temperature between trial types ( p > 0.05). These results suggest that high-intensity, short-duration RW may be a useful HT strategy for improving subsequent performance in cold environments.

Acute effects of the 4-4-8 breathing technique on arterial stiffness in healthy young men.

Increased arterial stiffness is a risk factor for cardiovascular disease. Slow, deep breathing decreases blood pressure related to arterial stiffness. The objective of the present study was to determine the acute effects of a single session of slow breathing on arterial stiffness, blood pressure, and cardiac autonomic function. Fifteen healthy men (20 ± 0 years) were administered (a) a slow breathing condition (12 consecutive breaths of 4 s of inhalation, 4 s of pause, and 8 s of exhalation through the nose, approximately 5 min per breath) and (b) a control, two-condition crossover design. Carotid-femoral artery pulse wave velocity (cfPWV), brachial-ankle PWV (baPWV), brachial blood pressure, high frequency (HF) and low frequency (LF) were measured at baseline, 30 min, 60 min and 24 h after respiratory control. Brachial-ankle PWV and brachial systolic pressure on the 4-4-8 breathing trial decreased after 30 min of respiratory control compared to baseline (p < 0.05), but did not change on the CON trial. Carotid-femoral PWV on both trials was unchanged; HF on the 4-4-8 breathing trial increased (p < 0.05) and LF decreased (p < 0.05) after 30 min of respiratory control compared to baseline, but was unchanged on the CON trial. These results suggest that slow breathing techniques may be effective in modulating autonomic function and improving arterial stiffness in healthy young adults.

Acute Caffeine Consumption Prior to Aerobic Exercise Does Not Influence Substrate Utilization in Recreationally Trained Males and Females

Introduction: This study aimed to examine if acute caffeine consumption influenced substrate utilization between recreationally trained males and females during submaximal aerobic exercise.Methods: Implementing a counter-balanced, crossover design study, 14 recreationally trained males (n = 7) and females (n = 7) consumed either 4 mg/kg of caffeine in 8 oz of water (CAFF) 60 min prior to aerobic exercise at varying submaximal intensities or the solitary consumption of 8 oz of water (CON). Substrate utilization was assessed via indirect calorimetry by measuring the respiratory exchange ratio. Results: There were no significant main effects for substrate utilization between sexes for the CAFF (p = .265) or CON (p = .253) trial. There were also no significant main effects for independent sex analysis between the two conditions (males, p = .917; females, p = .869).Conclusions: This study suggests no significant difference in substrate utilization between sexes when consuming caffeine. Although previous literature has indicated caffeine has the potential to increase fat utilization during moderate-intensity aerobic exercise, the current results revealed that caffeine also had no impact on substrate utilization when independently analyzing males and females.

Gastrointestinal function following endurance exercise under different environmental temperatures.

We determined the effects of different environmental temperatures on exercise-induced gastrointestinal (GI) damage and delayed gastric emptying (GE) rate. Eleven trained males completed three trials on different days, consisting of (1) exercise in a thermoneutral environment (CON, 23°C), (2) exercise in a hot environment (HOT, 35°C), and (3) exercise in a cold environment (COLD, 10°C). The subjects performed high-intensity interval-type endurance exercises in all trials. Blood intestinal fatty acid binding protein (I-FABP) levels was determine before and after exercise. We evaluated Tmax (time when the 13C-excretion/h reached a maximum level) as an indication of the GE rate during post-exercise. Rectal temperature during exercise was significantly higher (P < 0.001) in the HOT (38.7 ± 0.3°C) trial compared with the CON (38.2 ± 0.3°C) and COLD (38.2 ± 0.3°C) trials, with no significant difference between the CON and COLD trials. Plasma I-FABP level after exercise (relative to the pre-exercise level) were significantly greater (P = 0.005) in the HOT trial (92.9 ± 69.6%) than in the CON (37.2 ± 31.6%) and COLD (37.6 ± 41.8%) trials. However, there was no significant difference between the CON and COLD trials. Moreover, the Tmax was delayed significantly (P = 0.006) in the HOT trial compared with the CON and COLD trials, with no significant difference between the CON and COLD trials. GI function following endurance exercise was similar between thermoneutral and cold environments, while endurance exercise in a hot environment exacerbated GI function compared with thermoneutral and cold environments.

Acute aerobic exercise enhances cerebrovascular shear-mediated dilation in young adults: the role of cerebral shear.

Exercise-induced increases in shear rate (SR) acutely improve peripheral endothelial function, but the presence of this mechanism in cerebral arteries remains unclear. Thus, we evaluated shear-mediated dilation of the internal carotid artery (ICA), which is an index of cerebrovascular endothelial function, before and after exercise. Shear-mediated dilation was measured with 30 s of hypercapnia in 16 young adults before and 10 min after 30 min of sitting rest (CON) or three cycling exercises on four separate days. The target exercise intensity was 80% of oxygen uptake at the ventilatory threshold. To manipulate the ICA SR during exercise, participants breathed spontaneously (ExSB, SR increase) or hyperventilated without (ExHV, no increase in SR) or with ([Formula: see text], restoration of SR increase) addition of CO2 to inspiratory air. Shear-mediated dilation was calculated as a percent increase in diameter from baseline. Doppler ultrasound measures ICA velocity and diameter. The CON trial revealed that 30 min of sitting did not alter shear-mediated dilation (4.34 ± 1.37% to 3.44 ± 1.23%, P = 0.052). ICA dilation after exercise compared with preexercise levels increased in the ExSB trial (3.32 ± 1.37% to 4.74 ± 1.84%, P < 0.01), remained unchanged in the ExHV trial (4.07 ± 1.55% to 3.21 ± 1.48%, P = 0.07), but was elevated in the [Formula: see text] trial (3.35 ± 1.15% to 4.33 ± 2.12%, P = 0.04). Our results indicate that exercise-induced increases in cerebral shear may play a crucial role in improving cerebrovascular endothelial function after acute exercise in young adults.NEW & NOTEWORTHY We found that 30-min cycling (target intensity was 80% of the ventilatory threshold) with increasing shear of the internal carotid artery (ICA) enhanced transient hypercapnia-induced shear-mediated dilation of the ICA, reflecting improved cerebrovascular endothelial function. This enhancement of ICA dilation was diminished by suppressing the exercise-induced increase in ICA shear via hyperventilation. Our results indicate that increases in cerebral shear may be a key stimulus for improving cerebrovascular endothelial function after exercise in young adults.

The placebo effect of a pink non-caloric, artificially sweetened solution on strength endurance performance and psychological responses in trained individuals.

Background: The pink color enhances the perceived sweetness, increasing the individuals' expectation of the presence of sugar/carbohydrate in a beverage. Hence, it is plausible to speculate that providing a pink solution during exercise could induce an ergogenic benefit through a potential placebo effect. Aim: We examined whether ingesting a pink non-caloric, artificially sweetened solution can improve endurance strength exercise performance and psychological responses. Methods: Eighteen strength-trained individuals (34 ± 7 y; 1.74 ± 0.06 m; 79.86 ± 10.91 kg) completed three experimental trials in a randomized, single-blind, crossover counterbalanced fashion. In each trial, participants performed a 5-set strength endurance test at 70% of the one-repetition maximum in the bench press exercise, interspersed by 2 min. Before each set, participants ingested either a pink (PINK) or a transparent (TRANSP) non-caloric, artificially sweetened solution. A session without ingestion (CON) was also completed. Total number of repetitions and psychological responses such as motivation, emotional arousal, affect, and ratings of perceived exertion were obtained throughout the exercise protocol. Results: Total repetitions improved in PINK (60 ± 12 reps) compared to TRANSP (p = 0.03; 56 ± 10 reps; ES = 0.22; ±3.8%) and CON (p = 0.01; 56 ± 9 reps; ES = 0.33; ±6.6%), but no difference occurred between TRANSP and CON (p = 0.84; ES = 0.12; ±2.4%). Comparable responses were observed in motivation, emotional arousal, affect, and ratings of perceived exertion in PINK, TRANSP, and CON trials (all, p > 0.05), despite the greater total physical work performed in PINK trial. Conclusion: Ingesting a pink non-caloric, artificially sweetened solution improved strength endurance performance with comparable psychological responses. These results have implications for future nutritional studies and performance assessments in real-world sports scenarios.

Circulating fatty acid-binding protein 4 response to acute aerobic exercise in healthy men.

This study aimed to investigate the association between changes in FABP4 concentration and hormones and metabolites levels during and after acute low- and moderate-intensity aerobic exercise in healthy men. Eleven healthy men (24.4 ± 1.4 years) were administered three trials in random order: 1) low-intensity aerobic exercise trial (LE trial; acute exercise at 40% peak oxygen uptake [Vo2peak] + bed rest), 2) moderate-intensity aerobic exercise trial (ME trial: acute exercise at 60%Vo2peak + bed rest), and 3) bed rest (Con trial). Expired gas and venous blood samples were collected at baseline, and immediately, 30 and 60 min after exercise, and substrate oxidation and hormone, metabolite, and FABP4 concentrations were measured. FABP4 concentration did not increase during aerobic exercise in LE and ME trials (p > 0.05) However, FABP4 concentration significantly increased after the acute aerobic exercise in both the LE and ME trials (p ≤ 0.003). Increased FABP4 concentration after acute exercise was not correlated with adrenaline, noradrenaline, other hormone, and metabolite concentration changes. These findings suggest that FABP4 concentration increases after acute aerobic exercise independently of exercise intensity. Furthermore, increased FABP4 concentration after acute aerobic exercise may not be involved in the magnitude of adrenaline, noradrenaline, and lipolytic response.

Recovery with a fan-cooling jacket after exposure to high solar radiation during exercise in hot outdoor environments.

The study aimed to investigate the effect of body cooling with a fan-cooling jacket on body temperature responses during recovery after exercise when exposed to high solar radiation in a hot outdoor environment. Nine males cycled using ergometer until their rectal temperature increased to 38.50 °C in hot outdoor environments, followed by body cooling recovery in warm indoor environments. Subjects repeatedly performed the cycling exercise protocol, which consisted of one set of 5 min at a load of 1.5 watt/kg body weight and 15 min at a load of 2.0 watt/kg body weight at 60 rpm. Body cooling recovery consisted of cold water ingestion (10°C: CON) or cold water ingestion + wearing a fan-cooling jacket (FAN) until the rectal temperature decreased to 37.75°C. The time for the rectal temperature to reach 38.5°C did not differ between the two trials. The rate of decrease in rectal temperature at recovery tended to be higher in FAN trial than in CON trial (P = 0.082). The rate of decrease in tympanic temperature was higher in FAN trials than in CON trials (P = 0.002). The rate of decrease in mean skin temperature at the first 20 min of recovery was higher in FAN than in CON trial (P = 0.013). Body cooling recovery with a fan-cooling jacket in addition to cold water ingestion may be effective in reducing elevated tympanic and skin temperatures after exercise in the heat under a clear sky, but may be difficult to decrease rectal temperature.

Effects of mixed-cooling strategies on executive functions in simulated tennis in hot and humid conditions.

This study aimed to investigate the effects of mixed-cooling strategies, which combines external (cooling vest + neck cooled collar) and internal cooling (cold sports drink ingestion) on measures of executive function during simulated tennis in hot/humid conditions. In a counterbalanced design (randomised order), eight males undertook two trials [one with the mixed-cooling strategy, (MCOOL condition) and another without (CON condition)] in a climate chamber (36.5°C, 50% relative humidity). All subjects completed an intermittent treadmill protocol simulating a three-set tennis match with a 90-second break during odd-numbered games and 120-second breaks between sets, in accordance with the activity profile and International Tennis Federation rules. The mixed-cooling strategies were adopted before test and break time during the simulated tennis match. Stroop task, 2-back task, More-odd shifting task, gastrointestinal temperature (Tgi), skin temperature, blood lactic acid (BLA), heart rate, urine specific gravity (USG), sweat rate (SR), thermal sensation (TS) and perceived exertion (RPE) were measured. Results showed that the mean exercise time was longer in the MCOOL condition than in the CON condition. The SR was greater in CON trial compared with that in MCOOL trial. Results of two-way analysis of variance with repeated measures revealed that time×condition interactions were significant in BLA, Stroop response time, and switch cost of the more-odd shifting task. There were main effects of condition for Tgi, HR, TS, RPE, BLA, Stroop response time, and switch cost of the more-odd shifting task. In a hot/wet environment, pre- and intermittent mixed-cooling strategies can significantly improve exercise time and measures of executive function of tennis players in a simulated tennis match.

Walking Exercise Reduces Postprandial Lipemia but Does Not Influence Postprandial Hemorheological Properties and Oxidative Stress.

A higher postprandial triglycerides response and hemorheological abnormalities may increase the incidence of metabolic disorders and negatively interfere with the aging process. A single session of preprandial endurance exercise was found to be effective in reducing triglyceride levels after a high-fat diet. However, whether the exercise-induced reduction in postprandial triglyceride levels influences hemorheological indicators remains unknown. This study aims to investigate the effects of postprandial lipemia on hemorheological properties and oxidative stress. Eight healthy young male participants completed two experimental trials. On day 1, the participants were randomly assigned to walk for 1 h at 50% VO2max (EE trial) or rest (CON trial). On day 2, participants rested and consumed a high-fat meal in the morning. Results: The postprandial area under the curve (AUC) of plasma TG concentration was significantly lower in EE compared to CON (EE: 9.2 ± 1.9; CON: 10.9 ± 1.7 mmol/L·h−1; p = 0.013; Cohen’s d = 0.036). No significant difference was observed in hemorheological properties and MDA (p > 0.05). Endurance exercise effectively decreased postprandial TG concentration but did not influence the postprandial hemorheological properties and oxidative stress indicators.

Pre-cooling with ingesting a high-carbohydrate ice slurry on thermoregulatory responses and subcutaneous interstitial fluid glucose during heat exposure

The purpose of this study was to compare the effects of ingesting ice slurries with two different carbohydrate contents on body temperatures and the subcutaneous interstitial fluid glucose level during heat exposure. Seven physically active men underwent one of three interventions: the ingestion of 7.5 g/kg of a control beverage (CON: 26°C), a normal-carbohydrate ice slurry (NCIS: −1°C), or a high-carbohydrate ice slurry (HCIS: −5°C). The participants were monitored for a 120-min period that included 10 min of rest, 25 min of exposure to the experimental cooling intervention (during which the beverage was ingested), and 85 min of seated rest in a climate chamber (36°C, 50% relative humidity). The rectal temperature in the HCIS and NCIS trials was lower than that in the CON trial from 40 to 75 min. The infrared tympanic temperature was also lower in the HCIS and NCIS trials than in the CON trial from 20 to 50 min, whereas the deep thigh or mean skin temperatures were not significantly different among the three groups. From 90 to 120 min, the subcutaneous interstitial fluid glucose level in the NCIS trial was lower than that at 65 min; however, reductions were not seen in the HCIS and CON trials. These findings suggest that both HCIS ingestion and conventional NCIS ingestion were effective cooling strategies for reducing thermal strain, while HCIS ingestion may also enable a higher subcutaneous interstitial fluid glucose level to be maintained, ensuring an adequate supply of required muscle substrates.

Effect of High-Intensity, Intermittent, Short-Duration Re-Warming up on Cycling Sprint Performance.

The aim of this study was to investigate the effects of warming up again during half-time (i.e., re-warm up [RW]) with high-intensity, intermittent, short-duration exercise on cycling sprint performance. Participants (male, n = 10) performed intermittent cycling exercise for 40 min, followed by a 15-min half-time period with either rest only (control trials [CON]) or rest followed by a RW (three intervals of 3 s of maximal-effort cycling and 27 s of rest [HII]), after which participants performed the Cycling Intermittent-Sprint Protocol (CISP) to evaluate their sprint performance (17.0 ± 1.4°C, 44.2 ± 7.0% relative humidity). CISP intervals comprised 10 s rest, 5 s maximal effort cycling, and 105 s active recovery at 50% of the maximum oxygen uptake (VO2max) and were repeated 10 times. All participants performed both trial variations in randomized order. Peak power output of 5-s cycling sprints during the CISP were significantly higher in HII trials than those in CON trials (CON: 813 ± 109 W, HII: 836 ± 118 W, p < 0.05). Oxygen uptake, blood lactate concentration, and the rating of perceived exertion at the beginning of the second half after the RW were significantly higher in HII trials than those in CON trials (p < 0.05). These results demonstrate that the RW with intermittent, high-intensity, short-duration exercise improved subsequent cycling sprint performance in a thermoneutral environment and may represent a new useful RW strategy.

Exercise-induced Hemolysis And Iron Regulation In Response To Mechanical Stress

Hepcidin, a liver derived hormone, plays a role in iron regulation due to decreases in iron absorption and/or iron recycling. Exercise-induced hepcidin secretion is thought to be augmented by increased interleukin-6 (IL-6) and hemolysis (i.e., disruption of red blood cells). However, it is difficult to comprehend the contributions of hemolysis during exercise for hepcidin secretion. PURPOSE: To investigate the impact of exercise-induced hemolysis by a drop jump with concomitant repetitions of mechanical stress on hepcidin response. METHODS: Ten healthy males attended in this study [means ± SD, age: 24 ± 3 yrs, height: 170.6 ± 5.8 cm, body weight: 64.4 ± 7.2 kg]. Participants conducted two trials on different days, consisting of drop jump exercise to induce mechanical stress (DJ trial) or without exercise (CON trial). DJ trial consists of 100 bouts of drop jump from a 60 cm box, and each jump was conducted with their maximal effort. Blood samples were collected before trials, immediately, 1 h, 2 h and 3 h after completion of trials. The timing of blood sampling in CON trial was matched with DJ trial. A p value less than 0.05 was considered statistically significant. RESULTS: Baseline serum ferritin concentration did not significantly differ between DJ (73.1 ± 42.8 ng/mL) and CON trials (65.2 ± 31.6 ng/mL, p = 0.135). Serum iron and myoglobin concentration significantly increased and serum haptoglobin concentrations significantly decreased post-exercise in DJ trial (p < 0.05). Plasma IL-6 concentration did not increase in either trial (p > 0.05). Serum hepcidin concentration significantly increased 3 h after both trials (p < 0.05); however, the relative increase in serum hepcidin concentrations did not differ significantly between DJ and CON trials (77.4 ± 70.6% vs. 39.1 ± 51.5%, p = 0.184). CONCLUSIONS: Hemolysis was occurred following the drop jump exercise, while post-exercise IL-6 and hepcidin elevations were not different between the trials. Mechanical stress-induced hemolysis may not be sufficient to induce hepcidin elevation without IL-6 changes.

Rehydration Strategies For Prolonged Post-immersion Exercise Performance When Working In The Heat

Dehydration caused by cold-water immersion can reduce exercise capacity, especially in the heat where cardiovascular and thermoregulatory demands are increased. PURPOSE: The purpose of this study is to determine if post-immersion rehydration strategies maintain performance during sustained exercise in the heat. METHODS: Subjects (n = 5; 23 ± 2y; VO2max: 50.5 ± 6.2 ml/kg·min-1; BMI: 25.5 ± 2.2; BF: 10.7 ± 4.0%) completed one non-immersed control trial (CON) and two cold-water immersion (18.0 ± 0.2 °C) trials. Upon egress, subjects were either partially (HALF) or fully (FULL) rehydrated with water during a weighted ruck march (20.4 kg; 5.6 kph) at 50% VO2max in a warm environment (29.6 ± 0.1 °C; 50.1 ± 0.3% RH). CON trial received no water during the ruck march. Subjects then completed a 15-min intermittent exercise protocol (iEPT) with target speeds at 0, 25, 50 75, and 100% of their maximum sprint speed. The final 2-mins was a variable run in which they were instructed to cover as much distance as possible. Distance (km), heart rate (HR), core temperature (TC), rating of perceived exertion (RPE), and thermal comfort (TC) were recorded during the iEPT. Blood lactate (BL) was measured at the beginning and end of the iEPT. Data were analyzed with repeated measures one-way ANOVA. RESULTS: Initial hydration level and nude weight were not different between trials. During immersion, subjects lost 2.2 ± 0.5 and 2.3 ± 0.6% of body mass for HALF and FULL, respectively, and were rehydrated with 0.74 ± 0.3 L (HALF) and 1.6 ± 0.5 L (FULL) of water during the ruck. During the iEPT, distance covered during the targeted run (AVG: 1.90 ± 0.2 km; p = 0.11), and the variable run (AVG: 0.33 ± 0.08 km; p = 0.20) were not different between trials. HR during the iEPT was not different (p = 0.53) between trials, but TC was lower for FULL compared to CON (p = 0.047). BL was increased at the end of iEPT (p < 0.01) but was not different (p = 0.55) between trials. RPE (p = 0.38), TC (p = 0.11) were not different between trials. CONCLUSIONS: Both partial and full rehydration after cold-water immersion maintains performance when compared to a non-immersed control trial. Providing fluids during prolonged post-immersion exercise also maintains thermoregulation and comfort when working in the heat.

Leg Fidgeting Improves Executive Function following Prolonged Sitting with a Typical Western Meal: A Randomized, Controlled Cross-Over Trial.

Prolonged uninterrupted sitting and a typical Western meal, high in fat and refined sugar, can additively impair cognitive and cerebrovascular functions. However, it is unknown whether interrupting these behaviours, with a simple desk-based activity, can attenuate the impairment. The aim of this study was to determine whether regular leg fidgeting can off-set the detrimental effects of prolonged sitting following the consumption of a typical Western meal, on executive and cerebrovascular function. Using a randomized cross-over design, 13 healthy males consumed a Western meal and completed 180-min of prolonged sitting with leg fidgeting of 1 min on/4 min off (intervention [INT]) and without (control [CON]). Cognitive function was assessed pre and post sitting using the Trail Maker Test (TMT) parts A and B. Common carotid artery (CCA) blood flow, as an index of brain flow, was measured pre and post, and cerebral (FP1) perfusion was measured continuously. For TMT B the CON trial significantly increased (worsened) completion time (mean difference [MD] = 5.2 s, d = 0.38), the number of errors (MD = 3.33, d = 0.68) and cognitive fatigue (MD = 0.73, d = 0.92). Compared to CON, the INT trial significantly improved completion time (MD = 2.3 s, d = 0.97), and prevented declines in cognitive fatigue and a reduction in the number of errors. No significant changes in cerebral perfusion or CCA blood flow were found. Leg fidgeting for 1-min on/4-min off following a meal high in fats and refined sugars attenuated the impairment in executive function. This attenuation in executive function may not be caused by alterations in CCA blood flow or cerebral perfusion.

Effect of Electrical Muscle Stimulation on Vascular Endothelial Function during Prolonged Sitting.

While prolonged sedentary behaviors (SBs) increase cardiovascular disease (CVD) risk, interrupting prolonged sitting (PS) with frequent light exercise reduces arterial functional decline. Skeletal muscle electrical stimulation (EMS) enhances peripheral circulation through passive muscle contraction, suggesting that EMS reduces CVD risk by providing an alternative to active exercise for prolonged SBs. This study aimed to investigate the effects of EMS to skeletal muscles during PS on the endothelial function of the brachial artery (BA). Study participants included 12 healthy adult men who were subjected to 15 min of supine rest, followed by 1 h of PS only (control [CON] trial), or 20 min of EMS to the lower extremities at 50% of the maximum tolerance intensity during PS (EMS trial). Flow-mediated dilation (FMD) of the BA was measured before and 30 min after PS, and normalized FMD (nFMD) was calculated. The nFMD of the CON trial significantly decreased 30 min after PS completion (6.21% ± 1.13%) compared with that before PS (7.26% ± 0.73%), and there was no significant change in the EMS trial before and after PS. The EMS trial showed a significant increase in the nFMD 30 min after PS completion (1.14 ± 0.77) compared with that before PS (0.84 ± 0.43). However, no significant difference was observed in the CON trials. Passive contraction of the lower extremity muscles by EMS increases BA nFMD, suggesting that prolonged sedentary lower extremity EMS use may reduce the risk of vascular endothelial dysfunction.

Acute Effect of Repeated Sprint Exercise With Blood Flow Restriction During Rest Periods on Muscle Oxygenation.

PurposeThis study aimed to examine the effect of applying BFR during rest periods of repeated cycling sprints on muscle oxygenation.MethodsSeven active males performed 5 × 10-s maximal pedaling efforts with 40-s passive rest, with or without BFR application during rest period. BFR was applied for 30 s between sprints (between 5 and 35 s into rest) through a pneumatic pressure cuff inflated at 140 mmHg. Vastus lateralis muscle oxygenation was monitored using near-infrared spectroscopy. In addition, blood lactate concentration and heart rate were also evaluated.ResultsThe BFR trial showed significantly lower oxyhemoglobin (oxy-Hb) and tissue saturation (StO2) levels than the CON trial (P < 0.05). However, power output and blood lactate concentration did not significantly differ between the two trials (P > 0.05).ConclusionApplying BFR during rest periods of repeated cycling sprints decreased muscle oxygenation of active musculature, without interfering with power output during sprints.

Acute increase in arterial stiffness after swimming in cooler water.

Cold stimuli increase arterial stiffness, but it has not been explored whether arterial stiffness increases after swimming in cooler water. To investigate the effects of water temperature on changes in arterial stiffness after swimming, 13men participated in three trials of 20-min swimming in 25 and 30°C water (S25 and S30, respectively) and sitting at poolside (CON) in random order. There were no significant differences between the S25 and S30 trials in mean swimming distance (719 vs. 722m) and heart rate reserve during swimming (63% vs. 63%). Sublingual temperature was lower after swimming in 25°C water versus before swimming. Aortic pulse wave velocity (aortic PWV, an index of central arterial stiffness based on applanation tonometry) and brachial-ankle pulse wave velocity (baPWV, an index of systemic arterial stiffness based on air plethysmography) were higher 30min after versus before swimming in 25°C water. Aortic PWV recovered to pre-swimming levels by 60min after swimming in 25°C water, but baPWV was higher even at 60min after swimming. PWVs did not change in the CON and S30 trials. Systemic vascular resistance based on Doppler ultrasonography did not change, but forearm vascular resistance based on strain-gauge plethysmography was higher 30 and 60min after swimming in 25°C water. Heart rate was higher, but stroke volume was lower 30min after swimming in 25°C water, resulting in no detectable change in cardiac output. In conclusion, arterial stiffness increases acutely after moderate-intensity swimming in cooler water.

Effects of partial sleep deprivation after prolonged exercise on metabolic responses and exercise performance on the following day.

[Purpose]We determined the effect of partial sleep deprivation (PSD) after an exercise session on exercise performance on the following morning.[Methods]Eleven male athletes performed either a normal sleep trial (CON) or a PSD trial. On the first day (day 1), all subjects performed an exercise session consisting of 90 min of running (at 75% ) followed by 100 drop jumps. Maximal strength (MVC) was evaluated before and after exercise. In the CON trial, the sleep duration was 23:00–7:00, while in the PSD trial, the sleep duration was shortened to 40% of the regular sleep duration. On the following morning (day 2), MVC, the metabolic responses during 20 min of running (at 75% ), and time to exhaustion (TTE) at 85% were evaluated.[Results]On day 2, neither the MVC nor during 20 min of running differed significantly between the two trials. However, the respiratory exchange ratio was significantly lower in the PSD trial than in the CON trial (p = 0.01). Moreover, the TTE was significantly shorter in the PSD trial than in the CON trial (p = 0.01).[Conclusion]A single night of PSD after an exercise session significantly decreased endurance performance without significantly changing muscle strength or cardiopulmonary response.