Objectives: To understand sperm motility in adults and its association with lifestyle in an ethnic minority area in Southwest China. Methods: A hospital-based cross-sectional study to assess sperm motility in male adults was conducted at the Reproductive Health Center from January 2018 to May 2019. The data was collected with a questionnaire and semen quality was analyzed with Computer-Aided Sperm Analysis system (CASA). Analysis of covariance (ANCOVA) was used to measure the relationship between lifestyle factors and sperm motility. Results: A total of 349 people were recruited. Dietary celery intake was significantly related to the increase of sperm progressive motility and total motility (β = 7.00, 95% CI: 1.59, 12.42 and β = 7.26, 95% CI: 1.45, 13.07, respectively). Cola consumption was associated with increased sperm progressive motility (β = 9.71, 95% CI: 1.46, 17.96). Frequent use of plastic bags for meat food storage (β = -5.56, 95% CI: -10.61, -0.51), industry work (β = -5.64, 95% CI: -11.21, -0.07), organic disease (β = -6.14, 95% CI: -11.00, -1.28) and sedentary lifestyle (β = -5.92, 95% CI: -10.66, -1.17 for 3-5 h/d and β = -6.04, 95% CI: -11.60, -0.47 for ≥5 h/d, respectively) were related with the decreased sperm progressive motility. Meanwhile, using plastic bags for meat food storage (β = -6.37, 95% CI: -11.79, -0.95), industry work (β = -7.96, 95% CI: -13.94, -1.98) and sedentary lifestyle (β = -5.51, 95% CI: -10.60, -0.42 for 3-5 h/d and β = -6.03, 95% CI: -12.01, -0.06 for ≥5 h/d, respectively) were also risk factors for total motility. Conclusions: Some modifiable lifestyle factors such as job title, cola consumption, dietary celery intake, plastic bags for meat food storage, and sedentary hours were linked to male sperm motility, indicating that changing these lifestyles may improve it.