The healthcare supply chain is a network made up of various systems, processes, and elements that function and interact seamlessly to offer healthcare services and products. Food safety is an essential component of the healthcare supply chain. In the cattle industry, the healthcare supply chain contributes positively to the global economy through providing high-quality products such as milk and meat. Stress in cattle is one of main factor that cause low quality meat called “dark meat”. Numerous studies have been conducted on the development of different non-invasive methods based on Infrared Thermography Technology (IRT) to enhance the meat quality by detecting stress in cattle pre-slaughtering. These studies have the following issues: lack of automating in detecting body temperature of cattle and ignoring detecting stress with prediction dark meat incidence. The present study endeavors a new fully automated system for detecting stress, and dark meat incidence, incorporating the following new approaches: Multiview face detecting, Automatic eye localisation for detecting body temperature automatically employing computer vision and image processing, respectively. Furthermore, machine learning algorithms like Support Vector Machine (SVM), Logistic Regression (LR), Naïve Bayes (NB), and Decision Tree (DT) have been developed specifically for stress detection and the prediction of dark meat. To develop automated system, two forms of data were collected: statistical temperature and infrared thermal images. Infrared thermal images are used to develop Multiview face detection and Automatic eye localisation. Temperature data used to develop the machine learning model. Results reveal that Multiview face detection better than the current methods in term of Precision 0.99, Recall 0.91, F-score 0.95 with high True positive rate 0.90 and zero False-positive rate. Automatic eye localisation has high accuracy, with the following values: sensitivity 0.9780, precision 0.7212, F measure of 0.8024, and misclassification 0.0455. Lastly, results elaborate that the decision tree model can attain a notable level of accuracy in terms of specificity, recall, F-measure, and Area Under the Curve (AUC), all at an optimal rate of 98%.