The design and performance of a gain-switched, all acousto-optic (AO) Ti:Sapphire regenerative laser amplifier is presented. An AO Bragg cell is used to send pulses into and out of the amplifier cavity, and an AO modulator serves as an active isolation device. Pumping the high-Q amplifier with a short duration (∼40 ns) 532 nm pulse allows gain-switched operation in which the seed pulse dominates the amplified spontaneous emission; no Q switch is required. The amplified pulse energy is >110 μJ at a 4 kHz repetition rate, and the compressed pulse duration is ⩽50 fs. Detailed measurements are reported demonstrating that this design facilitates low-noise operation.