Comprehensive Exposure Assessment Research Articles

Increased population exposure to extreme droughts in Iberia due to 0.5ºC additional anthropogenic warming

Abstract This study investigates the effects of incremental global warming, specifically the transition from 1.5°C to 2.0°C, on drought conditions in the Iberian Peninsula (IP). Our findings confirm a substantial increase in the frequency and intensity of droughts in the IP due to anthropogenic climate change. We highlight the importance of temperature in drought representation and underscore the urgent need to limit global warming below 1.5°C, in line with international climate policies.
The analysis reveals that the exacerbation of drought conditions is more pronounced under higher emission scenarios, particularly RCP8.5, emphasizing the critical role of emission reduction in climate change mitigation. Furthermore, a substantial increase in affected land area and population exposure to drought is observed, especially under the high-emission scenario. Climate change emerges as the primary factor contributing to increased drought exposure, with emission reduction efforts offering potential mitigation. To overcome limitations associated with model uncertainties, a multi-model multi-variable ensemble approach was employed to enhance the regional specificity of the findings. This provides valuable insights for local climate adaptation and mitigation strategies.
Results suggest that mitigating anthropogenic warming by 0.5°C to achieve the 1.5°C warmer climate rather than 2.0°C may provide benefits for future drought risks and impacts in the IP and underscore the urgency of implementing stringent climate policies. By offering a comprehensive assessment of drought conditions and population exposure, this study informs decision-making and climate resilience strategies, emphasizing the need for immediate action to mitigate adverse impacts on ecosystems and human populations.

Integration of National Chemical Hazards Monitoring, Total Diet Study, and Human Biomonitoring Programmes for Food Safety Exposure Assessment in Singapore

Food safety and food security can impact the quality of human life, and these two aspects are interrelated alongside many influencing external factors. Global stressors such as climate change, recent pandemic, and geopolitical tensions have demonstrated tangible impacts on food security and safety. Food and food system innovation is a key strategy towards feeding the world in a more sustainable and climate-resilient manner. This paper highlights the use of a science-based risk assessment and management in Singapore’s food safety system, specifically in the integration of exposure assessment approaches to support evidence-based food safety risk analysis and decision-making. The use of complementary top-down and bottom-up exposure assessment approaches through the market monitoring programme, total diet study and human biomonitoring forms a comprehensive integrated exposure assessment strategy which can ultimately inform policy and measures in ensuring and securing a supply of safe food. The discussion on such application for chemical food safety in Singapore offers additional insights into the synergistic inter-relationships contributing to the exposure assessment associated with chemicals in food.

Urinary profiles of bisphenol S derivatives and their exposure pathway analysis in maternal and infant populations of Beijing

Bisphenol S (BPS) derivatives have potential reproductive developmental toxicity and have been found in the environment and in breast milk. The level of infant exposure and the source are currently unknown. In this study, we investigated BPS and six derivatives (together referred to as BPs) in urine samples from mothers and infants, indoor dust, breast milk and infant formula in Beijing, China. BPS, diphenyl sulfone (DPS) and 4-allyloxy-4′-hydroxydiphenyl sulfone (BPS-MAE) were the main BPs. Notably, the concentration of DPS in infants’ urine was higher than that of BPS, which warrants attention. Infants have higher daily intake levels than mothers. Exclusively breastfed infants have a higher risk of BPs exposure than exclusively formula-fed infants. For exclusive breastfed infants, the contribution of individual BPs through breast milk was 23.2% to 93.6%. While for exclusively formula-fed infants, the contribution of individual BPs through infant formula was 30.5% to 70.3%. The contribution of individual BPs through indoor dust was no more than 10%. The results suggesting that infants can be exposed to BPs through other pathways. This is the first comprehensive assessment of maternal and infants exposure to BPS derivatives, providing insights into the sources of infant exposure.

Comprehensive Assessment of Large-Scale Regional Fluvial Flood Exposure Using Public Datasets: A Case Study from China

China’s vulnerability to fluvial floods necessitates extensive exposure studies. Previous large-scale regional analyses often relied on a limited set of assessment indicators due to challenges in data acquisition, compounded by the scarcity of corresponding large-scale flood distribution data. The integration of public datasets offers a potential solution to these challenges. In this study, we obtained four key exposure indicators—population, built-up area (BA), road length (RL), and average gross domestic product (GDP)—and conducted an innovative analysis of their correlations both overall and locally. Utilising these indicators, we developed a comprehensive exposure index employing entropy-weighting and k-means clustering methods and assessed fluvial flood exposure across multiple return periods using fluvial flood maps. The datasets used for these indicators, as well as the flood maps, are primarily derived from remote sensing products. Our findings indicate a weak correlation between the various indicators at both global and local scales, underscoring the limitations of using singular indicators for a thorough exposure assessment. Notably, we observed a significant concentration of exposure and river flooding east of the Hu Line, particularly within the eastern coastal region. As flood return periods extended from 10 to 500 years, the extent of areas with flood depths exceeding 1 m expanded markedly, encompassing 2.24% of China’s territory. This expansion heightened flood risks across 15 administrative regions with varying exposure levels, particularly in Jiangsu (JS) and Shanghai (SH). This research provides a robust framework for understanding flood risk dynamics, advocating for resource allocation towards prevention and control in high-exposure, high-flood areas. Our findings establish a solid scientific foundation for effectively mitigating river flood risks in China and promoting sustainable development.

Impact of Kitchen Natural Gas Use on Indoor NO2 Levels and Human Health: A Case Study in Two European Cities

Natural gas (NG) is commonly used in kitchens, powering stoves, ovens, and other appliances. While it is known for its efficiency and convenience, NG contributes to the release of nitrogen dioxide (NO2) and can have significant implications for human health. In this study, the importance of the use of NG in kitchens on human exposure to NO2 was analyzed. An extensive literature review in the field was conducted, and the NO2 levels were assessed in kitchens with NG cookers in Aveiro and electric cookers in Porto, both in Portugal. Higher levels of NO2 were found in kitchens in Aveiro, where NO2 levels outdoors are lower than in Porto. This pollutant can spread to other rooms, especially when ventilation is lacking, which is particularly concerning during colder seasons and at night. As around 70% of the time is spent at home, this can have a significant impact on human exposure to NO2. Therefore, although Aveiro has low levels of NO2 outdoors, its population may be exposed to much higher levels of this pollutant than the Porto population, a city with air quality issues, but predominantly using electric cookers. This finding emphasizes the need for the stricter regulation of NG use indoors to protect human health and also suggests a shift in human health protection policies from mere monitoring/control of outdoor air quality to a comprehensive assessment of human exposure, including exposure to indoor air quality.

Comprehensive dietary exposure assessment of the Chinese population to organophosphate esters (OPEs): Results of the sixth China total diet study

Organophosphate esters (OPEs) are emerging pollutants, while data on their occurrence in foods and human dietary intake are limited. Based on the 6th China total diet study conducted in 2016–2019, this study implemented a comprehensive survey of OPEs in plant-derived foods of cereals, potatoes, legumes, fruits, vegetables, and further assessed dietary exposure from both plant- and animal-derived food. The sum concentrations of 15 OPEs in the plant-derived samples ranged from 0.567 to 106 ng/g ww. 2-Ethylhexyl diphenyl phosphate (EHDPP) (median: 1.14 ng/g ww) had the highest level in plant-derived foods, with a proportion of 35.6% in the total median OPEs. Regional distribution analysis showed a higher contamination of OPEs in plant-derived food from northern area of China. Estimated dietary intakes (EDIs) of ∑OPEs for Chinese population were from 109 ng/kg bw/day in Beijing to 1164 ng/kg bw/day in Gansu province, with mean and median of 296 and 222 ng/kg bw/day, respectively. Although animal-derived foods had higher levels of OPEs, plant-derived foods, specifically cereals, was the major source of dietary OPE intake. The EDIs were much lower than reference doses, which suggested the intakes of OPEs via food consumption could not cause significant health risks to the Chinese population at present.

Characterization of occupational inhalation exposures to particulate and gaseous straight and water-based metalworking fluids

Exposure assessments to metalworking fluids (MWF) is difficult considering the complex nature of MWF. This study describes a comprehensive exposure assessment to straight and water-based MWFs among workers from 20 workshops. Metal and organic carbon (OC) content in new and used MWF were determined. Full-shift air samples of inhalable particulate and gaseous fraction were collected and analysed gravimetrically and for metals, OC, and aldehydes. Exposure determinants were ascertained through observations and interviews with workers. Determinants associated with personal inhalable particulate and gaseous fractions were systematically identified using mixed models. Similar inhalable particle exposure was observed for straight and water-based MWFs (64–386 µg/m3). The gaseous fraction was the most important contributor to the total mass fraction for both straight (322–2362 µg/m3) and water-based MWFs (101–699 µg/m3). The aerosolized particles exhibited low metal content irrespective of the MWF type; however, notable concentrations were observed in the sumps potentially reaching hazardous concentrations. Job activity clusters were important determinants for both exposure to particulate and gaseous fractions from straight MWF. Current machine enclosures remain an efficient determinant to reduce particulate MWF but were inefficient for the gaseous fraction. Properly managed water-based MWF meaning no recycling and no contamination from hydraulic fluids minimizes gaseous exposure. Workshop temperature also influenced the mass fractions. These findings suggest that exposures may be improved with control measures that reduce the gaseous fraction and proper management of MWF.

Evaluation of Health Risks in the Blood Serum of People Working in the Welding Industry as a Result of Exposure to Heavy Metals in Diyala Governorate / Iraq

General Background: The welding industry is associated with significant health risks due to occupational exposure to heavy metals. Specific Background: Workers in this industry are particularly vulnerable to toxic metals such as lead, cadmium, copper, iron, and zinc, which can lead to severe health issues. Knowledge Gap: There is limited data on the specific health risks and heavy metal concentrations in welders' blood serum in Diyala Governorate, Iraq. Aims: This study aims to identify the primary health risks associated with welding-related heavy metal exposure and to explore strategies to mitigate these risks. Methods: The study was conducted in several industrial areas in Diyala Governorate, where blood samples were collected from 40 welders and 40 control participants (employees and students from Diyala University). An atomic absorption spectrophotometer was used to measure heavy metal concentrations in blood serum. Results: Statistical analysis revealed significant differences in heavy metal levels between welders and the control group (P Value < 0.05), indicating higher exposure among welders. However, no significant differences were found within welder subgroups based on age, smoking status, and duration of exposure. Novelty: This study provides the first comprehensive assessment of heavy metal exposure among welders in Diyala Governorate, highlighting the urgent need for improved protective measures. Implications: The findings underscore the necessity for environmental and health authorities to implement rapid interventions to protect welders from heavy metal toxicity. Current protective measures are inadequate, as evidenced by elevated levels of lead, cadmium, nickel, and zinc in welders' blood serum. Enhanced protective practices and stricter regulations are critical to reducing these occupational health risks. Highlights: 1. Higher levels of heavy metals found in welders' blood compared to controls.2. No significant differences among welder subgroups by age, smoking, exposure duration.3. Urgent need for better protective measures and interventions in welding industry. Keywords: Welding industry, heavy metals, occupational exposure, health risks, Diyala Governorate

Investigation of lifetime attributable risk associated with brain CT scans: A retrospective study in North Jordan

ObjectivesThis study aimed to investigate the lifetime attributable risk (LAR) associated with brain Computed Tomography (CT) scans, focusing on diverse dimensions, including radiation doses and technical factors. MethodsA retrospective study was conducted spanning from May 2021 to June 2022 across multiple hospitals in North Jordan. Data from 1505 adult patients undergoing brain CT scans were analyzed. The effective dose (E), calculated based on the dose length product (DLP) and tissue weighting factors, provided a comprehensive assessment of radiation exposure. Statistical analysis was employed to assess gender differences in mean effective doses across different age groups. LAR estimates were calculated for male and female patients. ResultsThe mean effective doses showed no significant gender differences, except in the 60–70 age group. However, LAR estimates revealed potential increased cancer risk, with values of 0.00029 for males and 0.000232 for females. These findings underscore the importance of scrutinizing radiation doses and technical parameters in brain CT examinations to mitigate potential risks. ConclusionThis study contributes valuable insights into the refinement of CT practices, emphasizing the necessity of optimizing radiation doses for patient safety in brain CT examinations. Addressing these factors is crucial to minimize the lifetime attributable risk associated with this imaging modality, ultimately enhancing patient care and outcomes.

Global Trends and Hotspots in Research on the Health Risks of Organophosphate Flame Retardants: A Bibliometric and Visual Analysis.

Organophosphate flame retardants (OPFRs) are compounds with a wide range of industrial and commercial applications and are mainly used as flame retardants and plasticizers. The global consumption of OPFRs has risen rapidly in recent decades, and they have been widely detected in environmental media. Unfortunately, OPFRs have been associated with many adverse health outcomes. The issue of the health risks of OPFRs is attracting increasing attention. Therefore, there is a need to review the current state of research and trends in this field to help researchers and policymakers quickly understand the field, identify new research directions, and allocate appropriate resources for further development of the OPFR health risk research field. This study statistically analyzed 1162 relevant publications included in the Web of Science Core Collection from 2003-2023. The internal and external features of the literature, such as publication trends, countries, authors, journals, and keywords, were quantitatively analyzed and visually presented to identify the research hotspots, compositions, and paradigms of the field and to horizontally and vertically analyze the development trends and structural evolution of the field. The development of the field can be divided into three stages, and the field entered a period of rapid development in 2016. China (649 papers) is the most prolific country, followed by the United States (188 papers). The authors STAPLETON HM and WANG Y have the highest combined impact. International collaboration between countries and researchers still needs to be strengthened. Science of The Total Environment is the most frequently published journal (162 papers), and Environmental Science and Technology is the most frequently cited journal (5285 citations). Endocrine disruption, developmental toxicity, and neurotoxicity are the health effects of greatest interest. Future research is expected to be multidisciplinary, and research hotspots may involve a comprehensive assessment of OPFR exposure in the population, exploration of the mechanisms of endocrine-disrupting effects and in vivo metabolic processes, and examination of the health effects of OPFR metabolites.

Assessment of Occupational Exposure to Inhalable Aerosols in an Instant Powdered Food Manufacturing Plant in Norway

BackgroundIn the food manufacturing industry, exposure to inhalable aerosols contributes to respiratory illnesses such as occupational asthma and rhinitis. However, there is a lack of comprehensive exposure assessment studies. This study evaluated occupational exposure to inhalable aerosols in an instant powdered food manufacturing plant during work operations involving dried food and powders. MethodsIn total, 50 workers from an instant powdered food manufacturing plant were recruited. Personal inhalable aerosol exposure measurements were taken for both full-shift and task-based activities. The concentrations of inhalable aerosols were analyzed to identify any variation within and across departments, as well as between seasons, handedness, and sex. ResultsIn total, 134 personal air samples were collected, and the particulate mass was determined gravimetrically. The concentrations of inhalable aerosols ranged from 0.1 to 27 mg/m3 for full-shift exposure measurements and 3.1 to 73 mg/m3 for task-based measurements. Statistically significant differences in mean aerosol concentrations were found across departments (A:B p < 0.001, A:C p < 0.05, B:C p < 0.001) and between seasons (p < 0.001). ConclusionThis study revealed high exposure to inhalable aerosols among workers, particularly those involved in manual weighing, mixing, and adding powders. The significant differences between departments highlight the specific activities contributing to increased inhalable aerosol concentrations. Seasonal variations were also evident, with autumn showing higher concentrations of inhalable aerosols in all departments compared with summer. These findings emphasize the importance of understanding the distribution of aerosol concentrations across different work tasks and departments, particularly during different seasons.

Comprehensive assessment of flood exposure in arid regions: Integrating GIS techniques and multi-method approaches – A case study of downstream swat river, Pakistan

Floods are among the most destructive natural disasters, causing extensive damage to human lives, property, and the environment. Pakistan is susceptible to natural calamities, such as the 2010 flood and the recent most 2022 flood, resulting in millions of people being impacted. The assessment of flood exposure is essential to plan effective mitigation and flood preparedness. Until now, an affective and inclusive method for calculating flood exposure in arid areas has not been developed. This paper proposes a new method in the selected area to calculate flood exposure in arid areas heavily affected by monsoons. This evaluates the exposure of settlements in the area based on four indicators: elevation of the area, slope and area topography, river density, and vegetation cover. Analytic Hierarchy Process (AHP) analysis was selected, and exposure maps were developed using ArcGIS. Due to heavy monsoon rains and climate change in the region, the risks of flood exposure become high. The results indicate low to high level exposure distribution in the area in terms of various social, economic, physical, and environmental factors. In the region, unexpected heavy precipitation during the monsoon season brings considerable losses to property and people exposed to floods due to river water overflow. In addition, the growing population and the unplanned construction of resident settlements in the floodplain is another threat. The analysis reveals that most riverside settlements have low elevation, with 55% of construction sites built in the floodplain indicating a high exposure rate ranging from 0.4 to 0.5. The study further discloses that numerous settlements have a high exposure to flood hazards increasing the likelihood of severe future impacts from flooding in the area. The local government must establish appropriate land use planning and zonation for construction in the area in light of these findings. Based on the results, the manuscript provides an inclusive analysis of the chosen indicators for the overall flood exposure of the area and results of low to high exposure, making this a valuable contribution to the field of exposure assessment. This study also provides thematic maps to the stakeholders for the effective management of exposure in the area to reduce the future flood susceptibility.

Burn Pit Smoke Condensate-Mediated Toxicity in Human Airway Epithelial Cells.

Burn pits are a method of open-air waste management that was common during military operations in Iraq, Afghanistan, and other regions in Southwest Asia. Veterans returning from deployment have reported respiratory symptoms, potentially from exposure to burn pit smoke, yet comprehensive assessment of such exposure on pulmonary health is lacking. We have previously shown that exposure to condensates from burn pit smoke emissions causes inflammation and cytotoxicity in mice. In this study, we explored the effects of burn pit smoke condensates on human airway epithelial cells (HAECs) to understand their impact on cellular targets in the human lung. HAECs were cultured at the air-liquid interface (ALI) and exposed to burn pit waste smoke condensates (plywood, cardboard, plastic, mixed, and mixed with diesel) generated under smoldering and flaming conditions. Cytotoxicity was evaluated by measuring transepithelial electrical resistance (TEER) and lactate dehydrogenase (LDH) release; toxicity scores (TSs) were quantified for each exposure. Pro-inflammatory cytokine release and modulation of gene expression were examined for cardboard and plastic condensate exposures. Burn pit smoke condensates generated under flaming conditions affected cell viability, with flaming mixed waste and plywood exhibiting the highest toxicity scores. Cardboard and plastic smoke condensates modulated cytokine secretion, with GM-CSF and IL-1β altered in more than one exposure group. Gene expression of detoxifying enzymes (ALDH1A3, ALDH3A1, CYP1A1, CYP1B1, NQO1, etc.), mucins (MUC5AC, MUC5B), and cytokines was affected by several smoke condensates. Particularly, expression of IL6 was elevated following exposure to all burn pit smoke condensates, and polycyclic aromatic hydrocarbon acenaphthene was positively associated with the IL-6 level in the basolateral media of HAECs. These observations demonstrate that exposure to smoke condensates of materials present in burn pits adversely affects HAECs and that aberrant cytokine secretion and altered gene expression profiles following burn pit material smoke exposure could contribute to the development of airway disease.

Comprehensive assessment of exposure and environmental risk of potentially toxic elements in surface water and sediment across China: A synthesis study

China faces a serious challenge with water pollution posed by potentially toxic elements (PTEs). Comprehensive and reliable environmental risk assessment is paramount for precise pollution prevention and control. Previous studies generally focused on a single environmental compartment within small regions, and the uncertainty in risk calculation is not fully considered. This study revealed the current exposure status of 11 PTEs in surface water and sediment across China using previously reported concentration data in 301 well-screened articles. Ecological and human health risks were evaluated and the uncertainty related to calculation parameters and exposure dataset were quantified. PTEs of high concern were further identified. Results showed Mn and Zn had the highest concentration levels, while Hg and Cd had the lowest concentrations in both surface water and sediment. Risk assessment of individual PTE showed that high-risk PTEs varied by risk receptors and environmental compartments. Nationwide, the probability of aquatic organisms being affected by Mn, Zn, Cu, and As in surface water exceeded 10 %. In sediment, Cd and Hg exhibited high and considerable risk, respectively. As was identified as the major PTE threatening human health as its carcinogenic risk was 1.45 × 10−4 through direct ingestion. Combined risk assessment showed the PTE mixture in surface water and sediment posed medium and high ecological risk with the risk quotient and potential ecological risk index of 1.76 and 558.36, respectively. Adverse health effects through incidental ingestion and dermal contact during swimming were negligible. This study provides a nationwide risk assessment of PTEs in China's aquatic environment and the robustness is verified, which can serve as a practical basis for policymakers to guide the early warning and precise management of water pollution.

Metal and oxidative potential exposure through particle inhalation and oxidative stress biomarkers: a 2-week pilot prospective study among Parisian subway workers

ObjectiveIn this pilot study on subway workers, we explored the relationships between particle exposure and oxidative stress biomarkers in exhaled breath condensate (EBC) and urine to identify the most relevant biomarkers for a large-scale study in this field.MethodsWe constructed a comprehensive occupational exposure assessment among subway workers in three distinct jobs over 10 working days, measuring daily concentrations of particulate matter (PM), their metal content and oxidative potential (OP). Individual pre- and post-shift EBC and urine samples were collected daily. Three oxidative stress biomarkers were measured in these matrices: malondialdehyde (MDA), 8-hydroxy-2′deoxyguanosine (8-OHdG) and 8-isoprostane. The association between each effect biomarker and exposure variables was estimated by multivariable multilevel mixed-effect models with and without lag times.ResultsThe OP was positively associated with Fe and Mn, but not associated with any effect biomarkers. Concentration changes of effect biomarkers in EBC and urine were associated with transition metals in PM (Cu and Zn) and furthermore with specific metals in EBC (Ba, Co, Cr and Mn) and in urine (Ba, Cu, Co, Mo, Ni, Ti and Zn). The direction of these associations was both metal- and time-dependent. Associations between Cu or Zn and MDAEBC generally reached statistical significance after a delayed time of 12 or 24 h after exposure. Changes in metal concentrations in EBC and urine were associated with MDA and 8-OHdG concentrations the same day.ConclusionAssociations between MDA in both EBC and urine gave opposite response for subway particles containing Zn versus Cu. This diverting Zn and Cu pattern was also observed for 8-OHdG and urinary concentrations of these two metals. Overall, MDA and 8-OHdG responses were sensitive for same-day metal exposures in both matrices. We recommend MDA and 8-OHdG in large field studies to account for oxidative stress originating from metals in inhaled particulate matter.

A Study on Assessment of Noise Exposure in the Port Industry: Implications for Occupational Health and Safety

The workplace today is getting more complex, as exposure to chemical, physical, and biological substances at work poses an increasing range of hazards and risks. Industrial hygiene is "that science and art devoted to the anticipation, recognition, evaluation, and control of those environmental factors or stresses arising in or from the workplace". Exposure assessment is the heart of industrial hygiene initiatives, which serves as the foundation for all other functional elements, including medical surveillance, exposure monitoring, engineering controls, administrative controls, and hazardous materials management. Ports are key hubs for worldwide trade, handling a wide range of cargo types with various machinery and equipment. The operational activities within these environments frequently generate high levels of noise, posing a potential hazard to the well-being of the workforce. The purpose of this study is to conduct a comprehensive assessment of noise exposure in the port industry at different locations and to propose appropriate mitigation strategies. The methodology involves the use of sound level measurements using a sound level meter in various port environments. A thorough examination was carried out to identify specific locations for the purpose of conducting noise monitoring. The survey facilitated the quantitative assessment of noise exposure. Based on the findings of this study, necessary recommendations are proposed to minimize noise exposure and protect the well-being of port workers. The insights gained will not just assist port workers but will also contribute to a broader awareness of sustainable and responsible industrial practices. Keywords: Noise exposure, Port industry, Noise assessment, Noise control methods, Personal protective equipment.

Bottled water safety evaluation: A comprehensive health risk assessment of oral exposure to heavy metals through deterministic and probabilistic approaches by Monte Carlo simulation

The consumption of bottled water has witnessed substantial global expansion in recent times. This study aimed to quantitatively evaluate the concentrations of eight heavy metals (As, Ba, Cd, Cr, Mn, Mo, Ni, and Zn) in 71 high-consumption bottled water brands in Iran. Non-carcinogenic and carcinogenic risk assessments were conducted using both deterministic and probabilistic approaches. Point estimation utilizing the Hazard Quotient (HQ) formula and sensitivity analysis employing the Monte Carlo Simulation (MCS) method through 10,000 repetitions in Oracle Crystal Ball® was used to ascertain the health risks associated with heavy metal exposure. Heavy metal concentrations were quantified through Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES). HQ point estimation results indicated that Cr exhibited the highest mean HQ value, whereas Cd demonstrated the lowest. In the probabilistic approach, the highest 95 percentile values were observed for Cr and Mo at 3.9E-01, while the lowest values were recorded for Cr and Mn at 1.10E-02. Heavy metal concentrations emerged as critical factors influencing non-carcinogenic and carcinogenic risks across all groups in the sensitivity analysis. The findings highlight the need for ongoing monitoring, research, and targeted regulations to address health risks from heavy metal exposure in bottled water and ensure public well-being.

Dioxin(-like)-Related Biological Effects through Integrated Chemical-wide and Metabolome-wide Analyses.

Dioxin(-like) exposures are linked to adverse health effects, including cancer. However, metabolic alterations induced by these chemicals remain largely unknown. Beyond known dioxin(-like) compounds, we leveraged a chemical-wide approach to assess chlorinated co-exposures and parent compound products [termed dioxin(-like)-related compounds] among 137 occupational workers. Endogenous metabolites were profiled by untargeted metabolomics, namely, reversed-phase chromatography with negative electrospray ionization (C18-negative) and hydrophilic interaction liquid chromatography with positive electrospray ionization (HILIC-positive). We performed a metabolome-wide association study to select dioxin(-like) associated metabolic features using a 20% false discovery rate threshold. Metabolic features were then characterized by pathway enrichment analyses. There are no significant features associated with polychlorinated dibenzo-p-dioxins (PCDDs), a subgroup of known dioxin(-like) compounds. However, 3,110 C18-negative and 2,894 HILIC-positive features were associated with at least one of the PCDD-related compounds. Abundant metabolic changes were also observed for polychlorinated dibenzofuran-related and polychlorinated biphenyl-related compounds. These metabolic features were primarily enriched in pathways of amino acids, lipid and fatty acids, carbohydrates, cofactors, and nucleotides. Our study highlights the potential of chemical-wide analysis for comprehensive exposure assessment beyond targeted chemicals. Coupled with advanced endogenous metabolomics, this approach allows for an in-depth exploration of metabolic alterations induced by environmental chemicals.

Comprehensive assessment of occupational exposure to microbial contamination in waste sorting facilities from Norway.

It is of upmost importance to contribute to fill the knowledge gap concerning the characterization of the occupational exposure to microbial agents in the waste sorting setting (automated and manual sorting). This study intends to apply a comprehensive field sampling and laboratory protocol (culture based-methods and molecular tools), assess fungal azole resistance, as well as to elucidate on potential exposure related health effects (cytotoxicity analyses). Skin-biota samples (eSwabs) were performed on workers and controls to identify other exposure routes. In personal filter samples the guidelines in one automated industry surpassed the guidelines for fungi. Seasonal influence on viable microbial contamination including fungi with reduced susceptibility to the tested azoles was observed, besides the observed reduced susceptibility of pathogens of critical priority (Mucorales and Fusarium sp.). Aspergillus sections with potential toxigenic effect and with clinical relevance were also detected in all the sampling methods. The results regarding skin-biota in both controls´ and workers´ hands claim attention for the possible exposure due to hand to face/mouth contact. This study allowed concluding that working in automated and manual waste sorting plants imply high exposure to microbial agents.

Aspiration efficiency and respiratory tract deposition of indoor suspended micro-particles during steady and transient breathings

The assessment of potential health risks from exposure to airborne contaminants and the inhalability of microparticles through human nasal breathing is crucial for the design of ventilation systems. In this study, using a standing computer-simulated person (CSP) directly linked to a numerical model of the respiratory tract, we investigated the aspiration efficiency (AE) of microparticles ranging between 1 and 80 μm under steady and transient breathing conditions. Two ventilation scenarios with displacement and mixed ventilation systems (DV and MV) were assumed for AE calculations. To determine the appropriate particle injection location, we investigated the position of highly inhaled particles corresponding to the breathing zone using a reverse particle-tracking simulation with reversed time progression. Additionally, the total and regional deposition fractions (TDF and RDF) of the inhaled microparticles on the respiratory wall surfaces were evaluated using the Lagrangian approach. The results indicate that the transient breathing cycle showed relatively higher AE and TDF rates compared to the assumed steady inhalation conditions, particularly for the size range 1–10 μm. An insignificant variation was observed in the AE and RDF results between different ventilation systems. However, microparticles in the MV room showed slightly higher AE than the DV system. Moreover, AE and penetration ratio were highly sensitive to the initially assumed particle density. For a comprehensive and accurate assessment of inhalation exposure to microparticles, we should predict the heterogeneous flow field formed around the human body and then analyze the AE and TDF in the respiratory tract during realistic transient breathing.